RchiOBHm_Chr2g0085081

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003701
Physical Location & Seq
Forward (+)
673794 .. 677001
3208 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46064

Sequence Viewer

Length: 2424 bp
ATGTACAACATACAGGCTGTGTTCCCGAACTTAACTTCGTTGACAGTACATAGGTGTGGTCGCCTAAGCTTCTTGTTTTCAATTTCTATGGCTAAAAGTCTTGTGGGACTCAAACATCTTGAGATATCCACTTGTGCAATCATAGAAGAGATTGTTTCATCAACAGGAGAACATGGTCAACAAAATATGGATAACATGTTCCCTAAGTTGGAAGATTTGAAGCTAAATGAACTTCCAACCCTTACCAGATTTTGCTCCGGAAGTTATATTCAACTTCTGTCCTTAGAAAAATTGGATATAGAAGGTTGTAGTAAACTGGGTGCATTCATTTCTGATAATGTTGCTATGAGTGGTGAAGATATTAGAATCTGCAAAGAAATTGAAGAGAAGGACTCTGAAGAGAACCTTGATGTTAACAGCAAGCTGAATACTGCACAATGCTTCCTCTTTGACGAAAAGATTGGTTTGCCTAACTTGACAAGCTTGATGGTTCACCGATGTGATGGTTTAAATTTCTTACTGTTATCTTCCATGGCAAGAAGTCTTGTAAAACTCAAACATCTTGTGATATCTGAATGTCAAAGAATGGAAGCAGTATTGACAAAAGAACACAGTGAAGAAAACAAGGATAACATGTTTCCTGAGTTAAAAGAATTGGACCTAAAGGATCTTCCTCATCTTGTTAGATTCTGCTCAGGAAATTATTTTGAATTTGCATCTATGGAGAGATTGCAATTAGAAAAGTGTGCTAAATTGGAGACATTCATCTGTAATCCCTCGAGTACCAGTATTGCAAGCCAAAAAGAAATGGAAAGAGTGGACAAGAATGAGAACCTTGAGACAGAAGTACCATACTTTCTTTTTGACAAAAAGGTAGGGCTTCCAAGGTTGGAGAGGTTGATTATCAATGAACTACCGAAGCTCACAACAATTTGGCACACTCAACTTGCTCCAAACTCTTTCCGCAGACTCAGAGACGTTACAGTGCAACGATGCAATGGTCTTAAATATATTTTTCAAGTCTCCATCATAGCCGGTGTTTTTCACCAGCTACAAAAGTTGATAGTGAAGGAGTGTGGAGTGGAGGAAATAGTTTCTCAGGAAGAGGGCATAGAAAAATTAATGCCTGATCAGTTCTTCCCAAAGCTAACATGTTTGGAATTTACAAGTCTATTCCAACTTGAGAGATTCTATCTAAAGATGCCTGCTATCAACTGGCCACTAAAAACATTAGTGTTGTCTGATTGTCAGAAAGTGGATATCTTTGCCGCGGAATCTTCAGGCCGTCAGAAAATACATGACTTGGGGAATCTGCGGGTTAAACAATATTATGTTCTATTTGAGAAGAGTTTATTTCCCAAACTGGAAGATTTGACCTTCAGCCCCGTGGACATCTGGTACGGTCGACCTGCCCATGAAAAAGCTCTTTTTCCTGCGACCGGTACAGATGGGTCCCTTCCACATTTGAGAACGTTGAGGCTACATGGAATGGAGAAGTTTATGCGTCTCTGGGAGAACACCGAACCTGCAGCTGGATCAGCTTTTCCACAGTTGAAAACTCTTGAGGTGAAGTTTTCTGGATTGACTAATCTAGAGTTGTCTGCAATATCCTTCCGGAATCTAACAACTATAGAAGTAACCTTTTGCTGGCGATTGCAATATTTGACCACTTATTCAGTAGCTCAAAGTCTCGTGCAACTCAAAACTTTGAAAGTTGACGAATGTAAAAATATGAAAGAAATCTTCACAAGCGAAGGAATGGGAGAAGGTGCAAGTAAATGTGAGATTGTTTTCAGGCAGTTGCAAACTTTGGAACTTGGTGATTTAATAAGTTTGGAACGGTTTTGCGCGAGCAACTGCAGAGTGAAGGTCCCAATCTTGGGAACTTTAAAGGTGAATTGGTGCCCGTTCAAATTGAAGATTTCCTCTGATAAGGTACTAGAAGTTGATCTGGAGTCTGATACTGAATCATGGTCGCAAAGCATGTCTATGCATGAACAGGACAACAGACGTTTGAGGGCGAAAGAAGGTAACAGCCTCCCGGAGACTCCGGTTGTTCTCGCGCTGCCTGAGACTGGTACTTCTAGCTCGTTGACTGCCAAGAAAACTGCTGATCTTCCTGCGGAGGTGATAGCCCAGCTTAATGCTCTGCTTGGAATTGACCTGGCAGCTCCCTCAGCCTATCAGCAACTCTCAGCACTCCTCCCATTTCTTCAATCAACTAGAAAGGAATGGGTAGTTGATCAAATAAGAGAAGCTCTTCAGAAATTGGCTCCTGCCGTGGACAAGAAGAGCTCTGAGCTAACTGCCCAACTTGAGGCGGGAAGGTCTGAGGTTATTGCCGGCAGCTCCCATATTGAGGCGGAATGTCAAGAAGTCATGGGCCGGGAGGCGGAACCGAGATTTGCTTCAGGCCCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

807

Amino Acids

91.14

Weight (kDa)

5.62

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 6 - 115 7.9e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 156 - 256 2.2e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 294 - 360 2.4e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 531 - 634 4.6e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1417, 1534
AccB1I GGYRCC 1 cut(s) 1902
AccI GTMKAC 1 cut(s) 1405
AccII CGCG 3 cut(s) 1271, 1850, 2063
AccIII TCCGGA 2 cut(s) 257, 1614
AciI CCGC 8 cut(s) 964, 1269, 1271, 1315, 2123, 2321, 2363, 2393
AclI AACGTT 1 cut(s) 1472
AclWI GGATC 2 cut(s) 675, 1543
AcoI YGGCCR 1 cut(s) 1217
AcsI RAATTY 3 cut(s) 511, 710, 1160
AcuI CTGAAG 5 cut(s) 417, 1263, 1363, 2246, 2394
AfaI GTAC 8 cut(s) 5, 48, 784, 849, 1400, 1444, 1938, 2080
AflIII ACRYGT 3 cut(s) 195, 633, 1151
AgeI ACCGGT 1 cut(s) 1439
AjnI CCWGG 2 cut(s) 2163, 2416
AleI CACNNNNGTG 1 cut(s) 498
AloI GAACNNNNNNTCC 2 cut(s) 182, 214
Alw21I GWGCWC 1 cut(s) 2297
Alw26I GTCTC 8 cut(s) 752, 833, 969, 1027, 1511, 1694, 2039, 2066
AlwI GGATC 2 cut(s) 675, 1543
Ama87I CYCGRG 1 cut(s) 778
Aor13HI TCCGGA 2 cut(s) 257, 1614
AoxI GGCC 4 cut(s) 1217, 1282, 2383, 2413
ApeKI GCWGC 4 cut(s) 1529, 2065, 2168, 2346
ApoI RAATTY 3 cut(s) 511, 710, 1160
AseI ATTAAT 1 cut(s) 1121
AsiGI ACCGGT 1 cut(s) 1439
Asp700I GAANNNNTTC 1 cut(s) 2259
AspLEI GCGC 2 cut(s) 1850, 2065
AspS9I GGNCC 5 cut(s) 658, 1452, 1870, 2383, 2414
AsuC2I CCSGG 2 cut(s) 2042, 2387
AsuHPI GGTGA 7 cut(s) 365, 485, 1037, 1579, 1832, 1906, 2140
AvaI CYCGRG 1 cut(s) 778
AvaII GGWCC 3 cut(s) 658, 1452, 1870
BaeGI GKGCMC 1 cut(s) 1907
BaeI ACNNNNGTAYC 2 cut(s) 831, 864
BalI TGGCCA 1 cut(s) 1219
BanI GGYRCC 1 cut(s) 1902
BanII GRGCYC 1 cut(s) 2297
BauI CACGAG 1 cut(s) 1691
Bbv12I GWGCWC 1 cut(s) 2297
BbvCI CCTCAGC 1 cut(s) 2176
BbvI GCAGC 4 cut(s) 1541, 2052, 2180, 2358
BccI CCATC 4 cut(s) 481, 497, 1034, 1442
BceAI ACGGC 2 cut(s) 1269, 2264
BciT130I CCWGG 2 cut(s) 2165, 2418
BclI TGATCA 2 cut(s) 1129, 2242
BcnI CCSGG 2 cut(s) 2042, 2387
BcoDI GTCTC 8 cut(s) 752, 833, 969, 1027, 1511, 1694, 2039, 2066
BfaI CTAG 4 cut(s) 1592, 1940, 2085, 2223
BfmI CTRYAG 3 cut(s) 1527, 1629, 1858
BfuAI ACCTGC 2 cut(s) 1417, 1534
BisI GCNGC 5 cut(s) 1269, 1530, 2066, 2169, 2347
BlsI GCNGC 5 cut(s) 1270, 1531, 2067, 2170, 2348
Bme1390I CCNGG 4 cut(s) 2042, 2165, 2387, 2418
Bme18I GGWCC 3 cut(s) 658, 1452, 1870
BmeT110I CYCGRG 1 cut(s) 778
BmgT120I GGNCC 5 cut(s) 658, 1452, 1870, 2383, 2414
BmiI GGNNCC 6 cut(s) 1453, 1454, 1872, 1904, 2274, 2397
BmrFI CCNGG 4 cut(s) 2042, 2165, 2387, 2418
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 3 cut(s) 725, 983, 1191
BmuI ACTGGG 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 377, 409
BpmI CTGGAG 1 cut(s) 1973
Bpu10I CCTNAGC 3 cut(s) 65, 694, 2176
BpuEI CTTGAG 5 cut(s) 140, 857, 1202, 1583, 2336
BpuMI CCSGG 2 cut(s) 2042, 2387
BsaJI CCNNGG 6 cut(s) 531, 884, 1269, 1386, 2280, 2416
BsaWI WCCGGW 4 cut(s) 257, 1439, 1614, 2050
Bse118I RCCGGY 3 cut(s) 1034, 1439, 2342
Bse1I ACTGG 5 cut(s) 321, 786, 1220, 1368, 2080
Bse3DI GCAATG 1 cut(s) 1003
BseAI TCCGGA 2 cut(s) 257, 1614
BseBI CCWGG 2 cut(s) 2165, 2418
BseDI CCNNGG 6 cut(s) 531, 884, 1269, 1386, 2280, 2416
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 9 cut(s) 633, 708, 985, 1112, 2061, 2190, 2208, 2289, 2322
BseNI ACTGG 5 cut(s) 321, 786, 1220, 1368, 2080
BseRI GAGGAG 1 cut(s) 2192
BseSI GKGCMC 1 cut(s) 1907
BseXI GCAGC 4 cut(s) 1541, 2052, 2180, 2358
BseYI CCCAGC 1 cut(s) 2136
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 3 cut(s) 1271, 1850, 2063
Bsh1285I CGRYCG 2 cut(s) 1405, 1440
BshFI GGCC 4 cut(s) 1219, 1284, 2385, 2415
BshNI GGYRCC 1 cut(s) 1902
BshTI ACCGGT 1 cut(s) 1439
BsiEI CGRYCG 2 cut(s) 1405, 1440
BsiHKAI GWGCWC 1 cut(s) 2297
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 8 cut(s) 258, 1035, 1440, 1615, 2042, 2051, 2343, 2386
BslFI GGGAC 3 cut(s) 120, 1438, 1856
BsmAI GTCTC 8 cut(s) 752, 833, 969, 1027, 1511, 1694, 2039, 2066
BsmBI CGTCTC 2 cut(s) 969, 1511
BsmFI GGGAC 3 cut(s) 120, 1438, 1856
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 4 cut(s) 1219, 1284, 2385, 2415
BsoBI CYCGRG 1 cut(s) 778
Bsp1286I GDGCHC 2 cut(s) 1907, 2297
Bsp13I TCCGGA 2 cut(s) 257, 1614
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
Bsp19I CCATGG 1 cut(s) 531
BspACI CCGC 8 cut(s) 964, 1269, 1271, 1315, 2123, 2321, 2363, 2393
BspANI GGCC 4 cut(s) 1219, 1284, 2385, 2415
BspCNI CTCAG 9 cut(s) 634, 707, 984, 1111, 2062, 2189, 2207, 2290, 2323
BspEI TCCGGA 2 cut(s) 257, 1614
BspFNI CGCG 3 cut(s) 1271, 1850, 2063
BspLI GGNNCC 6 cut(s) 1453, 1454, 1872, 1904, 2274, 2397
BspMAI CTGCAG 2 cut(s) 1531, 1862
BspMI ACCTGC 2 cut(s) 1417, 1534
BspPI GGATC 2 cut(s) 675, 1543
BspQI GCTCTTC 2 cut(s) 2265, 2285
BspT107I GGYRCC 1 cut(s) 1902
BsrDI GCAATG 1 cut(s) 1003
BsrFI RCCGGY 3 cut(s) 1034, 1439, 2342
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 321, 786, 1220, 1368, 2080
BssAI RCCGGY 3 cut(s) 1034, 1439, 2342
BssECI CCNNGG 6 cut(s) 531, 884, 1269, 1386, 2280, 2416
BssMI GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
BssSI CACGAG 1 cut(s) 1691
BssT1I CCWWGG 2 cut(s) 531, 884
Bst2BI CACGAG 1 cut(s) 1691
Bst2UI CCWGG 2 cut(s) 2165, 2418
Bst4CI ACNGT 7 cut(s) 46, 522, 614, 985, 1403, 1551, 1842
Bst6I CTCTTC 7 cut(s) 141, 378, 393, 1098, 1340, 2265, 2285
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 6 cut(s) 422, 796, 1206, 1649, 1852, 2344
BstDSI CCRYGG 4 cut(s) 531, 1269, 1386, 2280
BstFNI CGCG 3 cut(s) 1271, 1850, 2063
BstHHI GCGC 2 cut(s) 1850, 2065
BstKTI GATC 6 cut(s) 670, 1132, 1538, 1951, 2116, 2245
BstMAI GTCTC 8 cut(s) 752, 833, 969, 1027, 1511, 1694, 2039, 2066
BstMBI GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
BstMCI CGRYCG 2 cut(s) 1405, 1440
BstMWI GCNNNNNNNGC 2 cut(s) 1538, 2177
BstNI CCWGG 2 cut(s) 2165, 2418
BstNSI RCATGY 4 cut(s) 199, 637, 1155, 1987
BstSCI CCNGG 4 cut(s) 2040, 2163, 2385, 2416
BstSFI CTRYAG 3 cut(s) 1527, 1629, 1858
BstSLI GKGCMC 1 cut(s) 1907
BstUI CGCG 3 cut(s) 1271, 1850, 2063
BstV1I GCAGC 4 cut(s) 1541, 2052, 2180, 2358
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 4 cut(s) 1219, 1284, 2385, 2415
BtgI CCRYGG 4 cut(s) 531, 1269, 1386, 2280
BtsIMutI CAGTG 2 cut(s) 619, 990
BveI ACCTGC 2 cut(s) 1417, 1534
Cac8I GCNNGC 6 cut(s) 422, 796, 1206, 1649, 1852, 2344
CfoI GCGC 2 cut(s) 1850, 2065
Cfr10I RCCGGY 3 cut(s) 1034, 1439, 2342
Cfr13I GGNCC 5 cut(s) 658, 1452, 1870, 2383, 2414
Cfr42I CCGCGG 1 cut(s) 1272
CseI GACGC 1 cut(s) 1493
Csp6I GTAC 8 cut(s) 4, 47, 783, 848, 1399, 1443, 1937, 2079
CspAI ACCGGT 1 cut(s) 1439
CviQI GTAC 8 cut(s) 4, 47, 783, 848, 1399, 1443, 1937, 2079
DpnI GATC 6 cut(s) 669, 1131, 1537, 1950, 2115, 2244
DpnII GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
DraI TTTAAA 2 cut(s) 510, 1890
EaeI YGGCCR 1 cut(s) 1217
Eam1104I CTCTTC 7 cut(s) 141, 378, 393, 1098, 1340, 2265, 2285
EarI CTCTTC 7 cut(s) 141, 378, 393, 1098, 1340, 2265, 2285
EciI GGCGGA 2 cut(s) 2378, 2408
Ecl136II GAGCTC 1 cut(s) 2295
Eco130I CCWWGG 2 cut(s) 531, 884
Eco24I GRGCYC 1 cut(s) 2297
Eco32I GATATC 3 cut(s) 126, 570, 1261
Eco47I GGWCC 3 cut(s) 658, 1452, 1870
Eco53kI GAGCTC 1 cut(s) 2295
Eco57I CTGAAG 5 cut(s) 417, 1263, 1363, 2246, 2394
Eco88I CYCGRG 1 cut(s) 778
EcoICRI GAGCTC 1 cut(s) 2295
EcoO109I RGGNCCY 2 cut(s) 1452, 1870
EcoRII CCWGG 2 cut(s) 2163, 2416
EcoRV GATATC 3 cut(s) 126, 570, 1261
EcoT14I CCWWGG 2 cut(s) 531, 884
EcoT22I ATGCAT 1 cut(s) 1995
EcoT38I GRGCYC 1 cut(s) 2297
ErhI CCWWGG 2 cut(s) 531, 884
Esp3I CGTCTC 2 cut(s) 969, 1511
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 3 cut(s) 120, 1438, 1856
FauI CCCGC 2 cut(s) 1308, 2314
FbaI TGATCA 2 cut(s) 1129, 2242
FblI GTMKAC 1 cut(s) 1405
Fnu4HI GCNGC 5 cut(s) 1269, 1530, 2066, 2169, 2347
FriOI GRGCYC 1 cut(s) 2297
Fsp4HI GCNGC 5 cut(s) 1269, 1530, 2066, 2169, 2347
FspBI CTAG 4 cut(s) 1592, 1940, 2085, 2223
GlaI GCGC 2 cut(s) 1849, 2064
GluI GCNGC 5 cut(s) 1269, 1530, 2066, 2169, 2347
GsaI CCCAGC 1 cut(s) 2140
GsuI CTGGAG 1 cut(s) 1973
HaeIII GGCC 4 cut(s) 1219, 1284, 2385, 2415
HapII CCGG 8 cut(s) 258, 1035, 1440, 1615, 2042, 2051, 2343, 2386
HgaI GACGC 1 cut(s) 1493
HhaI GCGC 2 cut(s) 1850, 2065
Hin6I GCGC 2 cut(s) 1848, 2063
HinP1I GCGC 2 cut(s) 1848, 2063
HincII GTYRAC 6 cut(s) 42, 179, 415, 1406, 1717, 2094
HindII GTYRAC 6 cut(s) 42, 179, 415, 1406, 1717, 2094
HindIII AAGCTT 2 cut(s) 67, 481
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 8 cut(s) 258, 1035, 1440, 1615, 2042, 2051, 2343, 2386
HphI GGTGA 7 cut(s) 365, 485, 1037, 1579, 1832, 1906, 2140
HpyCH4III ACNGT 7 cut(s) 46, 522, 614, 985, 1403, 1551, 1842
HpyCH4IV ACGT 3 cut(s) 978, 1472, 2011
HpyF10VI GCNNNNNNNGC 2 cut(s) 1538, 2177
HpySE526I ACGT 3 cut(s) 978, 1472, 2011
HspAI GCGC 2 cut(s) 1848, 2063
KflI GGGWCCC 1 cut(s) 1452
Kpn2I TCCGGA 2 cut(s) 257, 1614
KroI GCCGGC 1 cut(s) 2342
KroNI GCCGGC 1 cut(s) 2344
Ksp22I TGATCA 2 cut(s) 1129, 2242
KspAI GTTAAC 1 cut(s) 415
KspI CCGCGG 1 cut(s) 1272
Kzo9I GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
LguI GCTCTTC 2 cut(s) 2265, 2285
LmnI GCTCC 5 cut(s) 260, 955, 2176, 2278, 2354
Lsp1109I GCAGC 4 cut(s) 1541, 2052, 2180, 2358
LweI GCATC 3 cut(s) 725, 983, 1191
MaeI CTAG 4 cut(s) 1592, 1940, 2085, 2223
MaeII ACGT 3 cut(s) 978, 1472, 2011
MaeIII GTNAC 3 cut(s) 979, 1636, 2030
MalI GATC 6 cut(s) 669, 1131, 1537, 1950, 2115, 2244
MboI GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 2 cut(s) 1907, 2297
MlsI TGGCCA 1 cut(s) 1219
MluNI TGGCCA 1 cut(s) 1219
MlyI GAGTC 5 cut(s) 102, 386, 963, 1964, 2041
MmeI TCCRAC 4 cut(s) 189, 260, 870, 1201
Mox20I TGGCCA 1 cut(s) 1219
Mph1103I ATGCAT 1 cut(s) 1995
MroI TCCGGA 2 cut(s) 257, 1614
MroNI GCCGGC 1 cut(s) 2342
MroXI GAANNNNTTC 1 cut(s) 2259
MscI TGGCCA 1 cut(s) 1219
MslI CAYNNNNRTG 3 cut(s) 54, 498, 1988
Msp20I TGGCCA 1 cut(s) 1219
MspA1I CMGCKG 2 cut(s) 1271, 1532
MspI CCGG 8 cut(s) 258, 1035, 1440, 1615, 2042, 2051, 2343, 2386
MspR9I CCNGG 4 cut(s) 2042, 2165, 2387, 2418
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 2 cut(s) 2165, 2418
MvnI CGCG 3 cut(s) 1271, 1850, 2063
MwoI GCNNNNNNNGC 2 cut(s) 1538, 2177
NaeI GCCGGC 1 cut(s) 2344
NciI CCSGG 2 cut(s) 2042, 2387
NcoI CCATGG 1 cut(s) 531
NdeII GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
NgoMIV GCCGGC 1 cut(s) 2342
NlaIV GGNNCC 6 cut(s) 1453, 1454, 1872, 1904, 2274, 2397
NsiI ATGCAT 1 cut(s) 1995
NspI RCATGY 4 cut(s) 199, 637, 1155, 1987
OliI CACNNNNGTG 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 778
PciI ACATGT 3 cut(s) 195, 633, 1151
PciSI GCTCTTC 2 cut(s) 2265, 2285
PctI GAATGC 1 cut(s) 323
PdiI GCCGGC 1 cut(s) 2344
PdmI GAANNNNTTC 1 cut(s) 2259
PfeI GAWTC 7 cut(s) 366, 687, 1188, 1274, 1309, 1618, 1967
PfoI TCCNGGA 1 cut(s) 2040
PinAI ACCGGT 1 cut(s) 1439
PkrI GCNGC 5 cut(s) 1270, 1531, 2067, 2170, 2348
PleI GAGTC 5 cut(s) 102, 386, 963, 1963, 2041
PpsI GAGTC 5 cut(s) 102, 386, 963, 1963, 2041
PpuMI RGGWCCY 2 cut(s) 1452, 1870
PscI ACATGT 3 cut(s) 195, 633, 1151
PshBI ATTAAT 1 cut(s) 1121
Psp124BI GAGCTC 1 cut(s) 2297
Psp1406I AACGTT 1 cut(s) 1472
Psp5II RGGWCCY 2 cut(s) 1452, 1870
Psp6I CCWGG 2 cut(s) 2163, 2416
PspFI CCCAGC 1 cut(s) 2136
PspGI CCWGG 2 cut(s) 2163, 2416
PspN4I GGNNCC 6 cut(s) 1453, 1454, 1872, 1904, 2274, 2397
PspPI GGNCC 5 cut(s) 658, 1452, 1870, 2383, 2414
PspPPI RGGWCCY 2 cut(s) 1452, 1870
PspXI VCTCGAGB 1 cut(s) 778
PstI CTGCAG 2 cut(s) 1531, 1862
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 1532
RsaI GTAC 8 cut(s) 5, 48, 784, 849, 1400, 1444, 1938, 2080
RsaNI GTAC 8 cut(s) 4, 47, 783, 848, 1399, 1443, 1937, 2079
RseI CAYNNNNRTG 3 cut(s) 54, 498, 1988
SacI GAGCTC 1 cut(s) 2297
SacII CCGCGG 1 cut(s) 1272
SalI GTCGAC 1 cut(s) 1404
SapI GCTCTTC 2 cut(s) 2265, 2285
SatI GCNGC 5 cut(s) 1269, 1530, 2066, 2169, 2347
Sau3AI GATC 6 cut(s) 667, 1129, 1535, 1948, 2113, 2242
Sau96I GGNCC 5 cut(s) 658, 1452, 1870, 2383, 2414
SchI GAGTC 5 cut(s) 102, 386, 963, 1964, 2041
ScrFI CCNGG 4 cut(s) 2042, 2165, 2387, 2418
SduI GDGCHC 2 cut(s) 1907, 2297
SfaNI GCATC 3 cut(s) 725, 983, 1191
SfcI CTRYAG 3 cut(s) 1527, 1629, 1858
Sfr274I CTCGAG 1 cut(s) 778
Sfr303I CCGCGG 1 cut(s) 1272
SgrBI CCGCGG 1 cut(s) 1272
SinI GGWCC 3 cut(s) 658, 1452, 1870
SlaI CTCGAG 1 cut(s) 778
SmiMI CAYNNNNRTG 3 cut(s) 54, 498, 1988
SmlI CTYRAG 6 cut(s) 119, 778, 836, 1181, 1562, 2315
SmoI CTYRAG 6 cut(s) 119, 778, 836, 1181, 1562, 2315
SsiI CCGC 8 cut(s) 964, 1269, 1271, 1315, 2123, 2321, 2363, 2393
SspI AATATT 2 cut(s) 1328, 1661
SspMI CTAG 4 cut(s) 1592, 1940, 2085, 2223
SstI GAGCTC 1 cut(s) 2297
StyD4I CCNGG 4 cut(s) 2040, 2163, 2385, 2416
StyI CCWWGG 2 cut(s) 531, 884
TaaI ACNGT 7 cut(s) 46, 522, 614, 985, 1403, 1551, 1842
TaiI ACGT 3 cut(s) 981, 1475, 2014
TaqI TCGA 2 cut(s) 779, 1405
TatI WGTACW 2 cut(s) 3, 46
TauI GCSGC 1 cut(s) 1271
TfiI GAWTC 7 cut(s) 366, 687, 1188, 1274, 1309, 1618, 1967
TscAI CASTG 2 cut(s) 619, 990
TseI GCWGC 4 cut(s) 1529, 2065, 2168, 2346
TspDTI ATGAA 8 cut(s) 147, 243, 316, 754, 924, 1431, 1748, 2010
TspRI CASTG 2 cut(s) 619, 990
VpaK11BI GGWCC 3 cut(s) 658, 1452, 1870
VspI ATTAAT 1 cut(s) 1121
XapI RAATTY 3 cut(s) 511, 710, 1160
XbaI TCTAGA 1 cut(s) 1591
XceI RCATGY 4 cut(s) 199, 637, 1155, 1987
XhoI CTCGAG 1 cut(s) 778
XmiI GTMKAC 1 cut(s) 1405
XmnI GAANNNNTTC 1 cut(s) 2259
XspI CTAG 4 cut(s) 1592, 1940, 2085, 2223
Zsp2I ATGCAT 1 cut(s) 1995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.