Rh7BG199800

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003701
Physical Location & Seq
Reverse (-)
16458740 .. 16462881
4142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG199800.1

Sequence Viewer

Length: 3855 bp
ATGGCGATTAGATTTACGGAGGCAATTCTTTTTAAAACTAAGGAGACAATTGAACTCGTAGGACGCCAAGTGGGATATGTAATTCACTGCAAAAGCAACCTTCAAAGACTGCAGGCTCAATTGGAAGAATTGAACGCCTCCCGGGAGGCGGTAGAGGATAAAGTTGTGGAAGCTGAAGACAAAGGTGAAGCAGTTCAACCAGATGTCCAGTTGTGGCTACAGGAAGTGGGCAACATCAATGCAGTGGCAGAGGAATTGTTGAAAGATGAAAACCAAGCCAAGCTGAAATGTCTCCATGGGTTTTGTCCTAATCTGACAATCCGTCATCACCTAGGCAGGAAATCAAGAAAATTGGAGTACCATCTTGTTGAACTCTATGAAAGACGAGAATTTCCCGTTATTTCTGTTATTCAGCCGCAAGAGCTTTGGGTTGAGTCCACCGGAGAGTACCTGGCCTTTCCATCAAGGACGTCAATTGTGGCCCAAATCATGGATGAACTTGAGAATCCTGATACTCATAGGATCGGGGTGTATGGAATTGAAGGTGTGGGTAAAACCACACTAGTAAAAGAAGTCTACAGGCAAGCTACAGAAGATAAAAAGTTATTTGACAATGTGGTTATCCTACTAGATGTTAAAGAAAATCCACATTTGGAAGGAATTCAAAACAAAATTTTAAATAAGTTAGATATGAAGATTTTTGACGATGAGAATTTAGATGGAAGAGCAAGTCGTACTTCTGCCGATATACAGGACAAAAACATCCTTGTAATTCTGGATGATGTTTGGGAACAAATTGACTTGGAGGCTCTCCAACTTCCAAGTGTGGCTACTTGTAAGATATTGCTGACATGTACAAGTAGAGAATCGTTATCTGGTATGAACACGCAGGAAGATTTTCAGCTTGACATCTTGGAAGAAGAAGAGGCTTGGTTTCTATTTAAGAAGAAGGCAGGCGATGTTGTTAAGAATTATGCTATACGAGAAGTAGCACTCCAAGTAGCCGAATGTTGTAGTGGCTTGCCTATTTTAGTTGTCACAGTTGCAAGTGCTTTGAGGAAACAAACTACTTTATCTCCATGGAAAGATGCATTGAGATTCCTAAAAAAATCTGATCACCAAGAATTGACAGAGAAGGCATACTTGTGTCTGGAGTGGAGTTACGTTCAGCTGAAGGATAAGGAACTTGAGCAGTTGTTCTTGCTATGTGGATTTGCTATATGGGGAAAGGATATTTATTTGCCAGCCTTGCTGAAATATAGTATGGGTTTGGGTCTCTTTAGAAAGGGATACACAGTGGAGGAAGCACAAAATGCATTGCTTACATGGGTTGGAAAACTTAAAGATTCTTGTCTACTGACAGACAGTGATGATAGCAGATATGTCAAAATGCATAACCTTGTGCGTGATGTTGCTAACCGGATTGCACTCAGAGATCGACACATCTTGTCATCAGTCGAAGATAGGGGAGATTATGAGTTTAAGGAATGGCTAGATAAGGATTTCTTTAAAAAGTGGACTATGAAATCTATACAATCCAGTACTGTTCCTGTGCTTTCTGAAGTACCTACGATATACCCCAAATTAAAACTGTTCTACTTGCACGGTACAGGTGATCTATCAGTGGAAATCCCAACAAACTTCTTTAGAGAGATTGAGGAACTAAAAGTGTTGTCTTTAACAAGACTGCATATGCTGTCACTACCTTCCTCTCTTCAGTACTTGAAGAATCTGCATGCCCTATTTTTAGATCAATGCATAGTGGGAGACACTGCTTTAGTTGGGGAGCTAAGTAACTTGGAAATTCTTAGCTTTTTCCAATCCAATGTCAAACAATTACCAAAGGAAATAGGGAAACTGACCCGTCTTCAGTTGCTGGATTTATCTGATTGCTCTGAACTCGAAGTTATTTCACCTGGTGTCATCTCAAGCTTGGAGAGACTACAAGAATTGAGAATGCGAAACAGCTTCAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTAGTGAGTTGAAGCATTTGTCTCAGCTATCTGTATTAGAGGTACATATTCCGAATACTGACATCCTTCCAGCAAACTTGTTCTCCCACGAGTTCGAAAGATTCAATATTGTTATTGGGGATGCATGGGTTGACAGATATACATCACGAGAGAAATTTATGGCTGAGACGACCCTCAAGACACTAAAACTCAAACTCAAACTCACTACCAGCATTAAAGAATTGGATGAAGGATTAATATGGCTGCTGGAGAGATGTGAAGACTTGTCCTTAAGTGAGACACAGAATGCTAATAATATTGACCATTATTTAGATACTGACGATTCTGAGCATCCGCAGCATCTGCAAGTCATAAACATCTATAAGATTGGTTTGCCCAACTTAACAAGATTGGCTGTTCACCGATGTGATGGTTTAAGTTTCTTATTGTCATCTTCCGTGGCAAAAAGTTTTGTACAACTTAAACATCTTCGGATATCTGAATGTCAAATCATGAAAGAGGTAGTATTGTCAGAAGGTCACGGTGAAGAAAACAAGGATAACTTGTTTCCGAAGTTAGAACAATTGGAGCTAAATGATCTTCCTTGTCTCACTAGATTCTGTTCAGGAAGTTATGTTGAGTTTGCAGCTCTGAAGAGGTCGCAATTGGAGAAGTGTCCTAAATTGGAGACATTCATCTTTAATCATTTGAGTAACAGAATTCAAATCTACAACGAAATTAAAGAAAGGGGCACAAATGATGAGAATGTTGAGACAGTTGCACCATACTTTCTTTTCGATGAAAAGGTGAAAACGTTGAGGCTGCATGGAATGGAGATCAAGCTCATGCGTCTCTCAGAAGAGAGCCCAGTTTTTCCACTGTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAACCTAAAGTTGTCTGTAATATCCTTTCGGAATCTAACAATTATAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCAAAGTTTAGTGGGACTGAAAAAATTGAAAGTTGATGAATGTAAAAATATGAAAGAAATCTTCACAAGTGAAGGAATTGAAGAAGATGCAAGTAATTGTGAGATTGTTTTCAGCCGGTTGCAACATTTGGAACTCAGTGATTTAATAAGTTTGGAACGGTTTTGCTCGAGAAATTGCATTGTGAAAGTCCCATCCTTGCAAAGTAGTGCTTTACAGGTAAATTGGTGCCCATTCCAGTTGAAGATTTCCACTGAAAGGGTACTAGAAGAAGTTTCCGATATGCAACAGGATGTACATAAGTCGCATACTGGTAGAGAAGAGGTTCTCGACACAACTGAGTCTGCTTGTAGCCTGGAAACAAATCAGAAACAGCACGTCCCAAACACCTCCGAAGTTTCTAAAGAACTCAAGTTTAATGCAGGAGAACTCCATGATGACAGACTTGTAAACAACGACGGGCCTCTATTGCCTGGCAATATTGCTGAAAGTAGTGAACATGGAGTCAAAGCTGTCATTAAAGAAACTCCTATCAATTTCTTGGAGCATGCTGAGGTTATGGACCTACGTCACAAGCAAATTGTCACCAATACGCCAAAGAATCTTCAGGAGGCTGATTTTGGCATGGCAGAGAGCCTCATTGAACCTTTGAATGCAAATGAAGGCAGACCACTTCAATTGAAACAACTTGATGGGGTGCAAACTGATTCATTGCTGCAAAGCATATCTGAGCTTGATGCAGACATAAAACATTTGCAGGAAGAATTAAGGGCCAAAGAAGCAGAGAGGAGAGATAAACAGCAAAGGCTTGCCGACAGACTTGCGACAGTTGATCGGTTGTCCGATTCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1284

Amino Acids

146.91

Weight (kDa)

5.25

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 159 - 319 7.2e-25 NB-ARC domain
LRR_14 PF23598 556 - 688 6e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 796 - 895 2.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 970 - 1077 9.5e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 3228
AccB7I CCANNNNNTGG 1 cut(s) 490
AccI GTMKAC 2 cut(s) 576, 1354
AciI CCGC 3 cut(s) 149, 416, 2345
AclI AACGTT 1 cut(s) 2804
AclWI GGATC 1 cut(s) 530
AcsI RAATTY 8 cut(s) 389, 660, 672, 712, 1803, 2165, 2709, 2877
AcuI CTGAAG 7 cut(s) 195, 1193, 1581, 1700, 1853, 2663, 3590
AcyI GRCGYC 2 cut(s) 64, 470
AdeI CACNNNGTG 1 cut(s) 1919
AfiI CCNNNNNNNGG 3 cut(s) 148, 490, 3258
AflII CTTAAG 1 cut(s) 2281
AflIII ACRYGT 1 cut(s) 851
AhdI GACNNNNNGTC 1 cut(s) 321
AhlI ACTAGT 1 cut(s) 562
AjiI CACGTC 1 cut(s) 3379
AjnI CCWGG 4 cut(s) 450, 1915, 3354, 3472
AleI CACNNNNGTG 1 cut(s) 2415
AlwI GGATC 1 cut(s) 530
AlwNI CAGNNNCTG 2 cut(s) 1876, 2353
Ama87I CYCGRG 2 cut(s) 141, 3169
AoxI GGCC 4 cut(s) 453, 480, 3461, 3771
ApeKI GCWGC 5 cut(s) 2254, 2347, 2636, 2812, 3715
ApoI RAATTY 8 cut(s) 389, 660, 672, 712, 1803, 2165, 2709, 2877
ArsI GACNNNNNNTTYG 2 cut(s) 2977, 3009
AseI ATTAAT 1 cut(s) 2246
Asp700I GAANNNNTTC 4 cut(s) 192, 660, 897, 3324
AspA2I CCTAGG 1 cut(s) 331
AspS9I GGNCC 4 cut(s) 481, 3461, 3562, 3771
AsuC2I CCSGG 2 cut(s) 142, 143
AsuII TTCGAA 1 cut(s) 2106
AvaI CYCGRG 2 cut(s) 141, 3169
AvaII GGWCC 1 cut(s) 3562
AvrII CCTAGG 1 cut(s) 331
BaeGI GKGCMC 2 cut(s) 2744, 3233
BanI GGYRCC 1 cut(s) 3228
BanII GRGCYC 1 cut(s) 2858
BauI CACGAG 2 cut(s) 2099, 2157
BbsI GAAGAC 3 cut(s) 183, 1859, 2277
BbvCI CCTCAGC 1 cut(s) 3552
BbvI GCAGC 5 cut(s) 2241, 2359, 2648, 2799, 3702
BccI CCATC 6 cut(s) 369, 469, 713, 2414, 3202, 3686
BcgI CGANNNNNNTGC 2 cut(s) 3803, 3837
BciT130I CCWGG 4 cut(s) 452, 1917, 3356, 3474
BciVI GTATCC 1 cut(s) 1283
BclI TGATCA 1 cut(s) 1114
BcnI CCSGG 2 cut(s) 142, 143
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 7 cut(s) 332, 563, 629, 1493, 2604, 2882, 3266
BfmI CTRYAG 4 cut(s) 110, 218, 577, 588
BfrI CTTAAG 1 cut(s) 2281
BfuI GTATCC 1 cut(s) 1283
BisI GCNGC 6 cut(s) 416, 2255, 2348, 2637, 2813, 3716
BlnI CCTAGG 1 cut(s) 331
BlsI GCNGC 6 cut(s) 417, 2256, 2349, 2638, 2814, 3717
BmcAI AGTACT 2 cut(s) 1543, 1721
Bme1390I CCNGG 6 cut(s) 142, 143, 452, 1917, 3356, 3474
Bme18I GGWCC 1 cut(s) 3562
BmeRI GACNNNNNGTC 1 cut(s) 321
BmeT110I CYCGRG 2 cut(s) 141, 3169
BmgBI CACGTC 1 cut(s) 3379
BmgT120I GGNCC 4 cut(s) 481, 3461, 3562, 3771
BmiI GGNNCC 1 cut(s) 3230
BmrFI CCNGG 6 cut(s) 142, 143, 452, 1917, 3356, 3474
BmrI ACTGGG 1 cut(s) 2852
BmsI GCATC 6 cut(s) 1078, 2122, 2350, 2359, 3079, 3727
BmuI ACTGGG 1 cut(s) 2852
BoxI GACNNNNGTC 1 cut(s) 3567
BpiI GAAGAC 3 cut(s) 183, 1859, 2277
BpmI CTGGAG 2 cut(s) 1172, 2279
Bpu10I CCTNAGC 1 cut(s) 3552
Bpu14I TTCGAA 1 cut(s) 2106
BpuEI CTTGAG 5 cut(s) 521, 1208, 1912, 2171, 3395
BpuMI CCSGG 2 cut(s) 142, 143
BsaBI GATNNNNATC 1 cut(s) 2152
BsaHI GRCGYC 2 cut(s) 64, 470
BsaI GGTCTC 1 cut(s) 1280
BsaJI CCNNGG 5 cut(s) 141, 295, 331, 1079, 2448
BsaWI WCCGGW 2 cut(s) 440, 1419
BsaXI ACNNNNNCTCC 4 cut(s) 1060, 1090, 2078, 2108
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 490, 3258
Bse118I RCCGGY 1 cut(s) 3117
Bse1I ACTGG 5 cut(s) 208, 1539, 2858, 3238, 3316
Bse3DI GCAATG 2 cut(s) 1316, 3710
Bse8I GATNNNNATC 1 cut(s) 2152
BseBI CCWGG 4 cut(s) 452, 1917, 3356, 3474
BseDI CCNNGG 5 cut(s) 141, 295, 331, 1079, 2448
BseGI GGATG 9 cut(s) 499, 762, 784, 2073, 2137, 2242, 2341, 3194, 3298
BseJI GATNNNNATC 1 cut(s) 2152
BseLI CCNNNNNNNGG 3 cut(s) 148, 490, 3258
BseMI GCAATG 2 cut(s) 1316, 3710
BseMII CTCAG 9 cut(s) 1444, 2048, 2166, 2328, 2859, 3151, 3330, 3543, 3720
BseNI ACTGG 5 cut(s) 208, 1539, 2858, 3238, 3316
BseRI GAGGAG 1 cut(s) 3802
BseSI GKGCMC 2 cut(s) 2744, 3233
BseXI GCAGC 5 cut(s) 2241, 2359, 2648, 2799, 3702
BshFI GGCC 4 cut(s) 455, 482, 3463, 3773
BshNI GGYRCC 1 cut(s) 3228
BsiHKCI CYCGRG 2 cut(s) 141, 3169
BsiSI CCGG 4 cut(s) 142, 441, 1420, 3118
BslFI GGGAC 3 cut(s) 3030, 3176, 3365
BslI CCNNNNNNNGG 3 cut(s) 148, 490, 3258
BsmBI CGTCTC 2 cut(s) 2171, 2846
BsmFI GGGAC 3 cut(s) 3030, 3176, 3365
BsmI GAATGC 3 cut(s) 1962, 2302, 3658
BsnI GGCC 4 cut(s) 455, 482, 3463, 3773
Bso31I GGTCTC 1 cut(s) 1280
BsoBI CYCGRG 2 cut(s) 141, 3169
Bsp119I TTCGAA 1 cut(s) 2106
Bsp1286I GDGCHC 3 cut(s) 2744, 2858, 3233
Bsp1407I TGTACA 3 cut(s) 854, 2464, 3295
Bsp143I GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
Bsp19I CCATGG 2 cut(s) 295, 1079
BspACI CCGC 3 cut(s) 149, 416, 2345
BspANI GGCC 4 cut(s) 455, 482, 3463, 3773
BspCNI CTCAG 9 cut(s) 1443, 2047, 2167, 2329, 2858, 3150, 3331, 3544, 3721
BspHI TCATGA 1 cut(s) 2502
BspLI GGNNCC 1 cut(s) 3230
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 718
BspT104I TTCGAA 1 cut(s) 2106
BspT107I GGYRCC 1 cut(s) 3228
BspTI CTTAAG 1 cut(s) 2281
BspTNI GGTCTC 1 cut(s) 1280
BsrDI GCAATG 2 cut(s) 1316, 3710
BsrFI RCCGGY 1 cut(s) 3117
BsrGI TGTACA 3 cut(s) 854, 2464, 3295
BsrI ACTGG 5 cut(s) 208, 1539, 2858, 3238, 3316
BssAI RCCGGY 1 cut(s) 3117
BssECI CCNNGG 5 cut(s) 141, 295, 331, 1079, 2448
BssMI GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
BssNI GRCGYC 2 cut(s) 64, 470
BssSI CACGAG 2 cut(s) 2099, 2157
BssT1I CCWWGG 3 cut(s) 295, 331, 1079
Bst2BI CACGAG 2 cut(s) 2099, 2157
Bst2UI CCWGG 4 cut(s) 452, 1917, 3356, 3474
Bst6I CTCTTC 6 cut(s) 718, 918, 1719, 2639, 2844, 3315
BstACI GRCGYC 2 cut(s) 64, 470
BstAFI CTTAAG 1 cut(s) 2281
BstAPI GCANNNNNTGC 2 cut(s) 1313, 2353
BstAUI TGTACA 3 cut(s) 854, 2464, 3295
BstBI TTCGAA 1 cut(s) 2106
BstDSI CCRYGG 3 cut(s) 295, 1079, 2448
BstF5I GGATG 9 cut(s) 499, 762, 784, 2073, 2137, 2242, 2341, 3194, 3298
BstKTI GATC 8 cut(s) 525, 1117, 1438, 1618, 1753, 2590, 2829, 3835
BstMBI GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
BstMWI GCNNNNNNNGC 7 cut(s) 421, 1249, 1313, 2347, 2353, 3469, 3779
BstNI CCWGG 4 cut(s) 452, 1917, 3356, 3474
BstNSI RCATGY 3 cut(s) 855, 1739, 3551
BstPAI GACNNNNGTC 1 cut(s) 3567
BstSCI CCNGG 6 cut(s) 140, 141, 450, 1915, 3354, 3472
BstSFI CTRYAG 4 cut(s) 110, 218, 577, 588
BstSLI GKGCMC 2 cut(s) 2744, 3233
BstV1I GCAGC 5 cut(s) 2241, 2359, 2648, 2799, 3702
BstV2I GAAGAC 3 cut(s) 183, 1859, 2277
BsuI GTATCC 1 cut(s) 1283
BsuRI GGCC 4 cut(s) 455, 482, 3463, 3773
BtgI CCRYGG 3 cut(s) 295, 1079, 2448
BtgZI GCGATG 1 cut(s) 972
BtrI CACGTC 1 cut(s) 3379
BtsCI GGATG 9 cut(s) 499, 762, 784, 2073, 2137, 2242, 2341, 3194, 3298
BtsI GCAGTG 3 cut(s) 85, 249, 1770
BtsIMutI CAGTG 9 cut(s) 85, 249, 1302, 1372, 1629, 1770, 2867, 3145, 3252
CaiI CAGNNNCTG 2 cut(s) 1876, 2353
CciI TCATGA 1 cut(s) 2502
Cfr10I RCCGGY 1 cut(s) 3117
Cfr13I GGNCC 4 cut(s) 481, 3461, 3562, 3771
Cfr9I CCCGGG 1 cut(s) 141
CseI GACGC 2 cut(s) 72, 2828
CsiI ACCWGGT 1 cut(s) 1915
CspCI CAANNNNNGTGG 2 cut(s) 3660, 3695
DpnI GATC 8 cut(s) 524, 1116, 1437, 1617, 1752, 2589, 2828, 3834
DpnII GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
DraI TTTAAA 3 cut(s) 34, 678, 1510
DraIII CACNNNGTG 1 cut(s) 1919
DriI GACNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 6 cut(s) 718, 918, 1719, 2639, 2844, 3315
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 6 cut(s) 718, 918, 1719, 2639, 2844, 3315
Eco130I CCWWGG 3 cut(s) 295, 331, 1079
Eco24I GRGCYC 1 cut(s) 2858
Eco31I GGTCTC 1 cut(s) 1280
Eco32I GATATC 1 cut(s) 2487
Eco47I GGWCC 1 cut(s) 3562
Eco57I CTGAAG 7 cut(s) 195, 1193, 1581, 1700, 1853, 2663, 3590
Eco88I CYCGRG 2 cut(s) 141, 3169
EcoRI GAATTC 2 cut(s) 660, 2709
EcoRII CCWGG 4 cut(s) 450, 1915, 3354, 3472
EcoRV GATATC 1 cut(s) 2487
EcoT14I CCWWGG 3 cut(s) 295, 331, 1079
EcoT22I ATGCAT 5 cut(s) 1093, 1318, 1395, 1760, 2137
EcoT38I GRGCYC 1 cut(s) 2858
ErhI CCWWGG 3 cut(s) 295, 331, 1079
Esp3I CGTCTC 2 cut(s) 2171, 2846
FaqI GGGAC 3 cut(s) 3030, 3176, 3365
FauNDI CATATG 1 cut(s) 1692
FbaI TGATCA 1 cut(s) 1114
FblI GTMKAC 2 cut(s) 576, 1354
Fnu4HI GCNGC 6 cut(s) 416, 2255, 2348, 2637, 2813, 3716
FokI GGATG 9 cut(s) 506, 749, 791, 2060, 2144, 2249, 2328, 3181, 3305
FriOI GRGCYC 1 cut(s) 2858
Fsp4HI GCNGC 6 cut(s) 416, 2255, 2348, 2637, 2813, 3716
FspBI CTAG 7 cut(s) 332, 563, 629, 1493, 2604, 2882, 3266
GluI GCNGC 6 cut(s) 416, 2255, 2348, 2637, 2813, 3716
GsuI CTGGAG 2 cut(s) 1172, 2279
HaeIII GGCC 4 cut(s) 455, 482, 3463, 3773
HapII CCGG 4 cut(s) 142, 441, 1420, 3118
HgaI GACGC 2 cut(s) 72, 2828
Hin1I GRCGYC 2 cut(s) 64, 470
HincII GTYRAC 1 cut(s) 2143
HindII GTYRAC 1 cut(s) 2143
HindIII AAGCTT 1 cut(s) 1930
HpaII CCGG 4 cut(s) 142, 441, 1420, 3118
Hpy166II GTNNAC 8 cut(s) 438, 577, 1355, 1518, 2143, 2410, 3451, 3497
Hpy8I GTNNAC 8 cut(s) 438, 577, 1355, 1518, 2143, 2410, 3451, 3497
Hpy99I CGWCG 1 cut(s) 3461
HpyCH4IV ACGT 5 cut(s) 470, 1164, 2804, 3378, 3568
HpyF10VI GCNNNNNNNGC 7 cut(s) 421, 1249, 1313, 2347, 2353, 3469, 3779
HpySE526I ACGT 5 cut(s) 470, 1164, 2804, 3378, 3568
Hsp92I GRCGYC 2 cut(s) 64, 470
Ksp22I TGATCA 1 cut(s) 1114
Kzo9I GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 3 cut(s) 1786, 2578, 3544
Lsp1109I GCAGC 5 cut(s) 2241, 2359, 2648, 2799, 3702
LweI GCATC 6 cut(s) 1078, 2122, 2350, 2359, 3079, 3727
MabI ACCWGGT 1 cut(s) 1915
MaeI CTAG 7 cut(s) 332, 563, 629, 1493, 2604, 2882, 3266
MaeII ACGT 5 cut(s) 470, 1164, 2804, 3378, 3568
MaeIII GTNAC 9 cut(s) 1036, 1160, 1698, 1793, 2528, 2702, 2956, 3569, 3583
MalI GATC 8 cut(s) 524, 1116, 1437, 1617, 1752, 2589, 2828, 3834
MboI GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
MfeI CAATTG 6 cut(s) 48, 119, 474, 2573, 2654, 3677
MhlI GDGCHC 3 cut(s) 2744, 2858, 3233
MlyI GAGTC 3 cut(s) 443, 3350, 3513
MmeI TCCRAC 3 cut(s) 838, 1312, 2853
Mph1103I ATGCAT 5 cut(s) 1093, 1318, 1395, 1760, 2137
MroXI GAANNNNTTC 4 cut(s) 192, 660, 897, 3324
MslI CAYNNNNRTG 2 cut(s) 1144, 2415
MspA1I CMGCKG 1 cut(s) 1171
MspCI CTTAAG 1 cut(s) 2281
MspI CCGG 4 cut(s) 142, 441, 1420, 3118
MspR9I CCNGG 6 cut(s) 142, 143, 452, 1917, 3356, 3474
MunI CAATTG 6 cut(s) 48, 119, 474, 2573, 2654, 3677
Mva1269I GAATGC 3 cut(s) 1962, 2302, 3658
MvaI CCWGG 4 cut(s) 452, 1917, 3356, 3474
MwoI GCNNNNNNNGC 7 cut(s) 421, 1249, 1313, 2347, 2353, 3469, 3779
NciI CCSGG 2 cut(s) 142, 143
NcoI CCATGG 2 cut(s) 295, 1079
NdeI CATATG 1 cut(s) 1692
NdeII GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
NlaIV GGNNCC 1 cut(s) 3230
NmuCI GTSAC 5 cut(s) 1036, 1698, 2528, 3569, 3583
NsiI ATGCAT 5 cut(s) 1093, 1318, 1395, 1760, 2137
NspI RCATGY 3 cut(s) 855, 1739, 3551
NspV TTCGAA 1 cut(s) 2106
OliI CACNNNNGTG 1 cut(s) 2415
PaeI GCATGC 2 cut(s) 1739, 3551
PaeR7I CTCGAG 1 cut(s) 3169
PagI TCATGA 1 cut(s) 2502
PciI ACATGT 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 718
PctI GAATGC 3 cut(s) 1962, 2302, 3658
PdmI GAANNNNTTC 4 cut(s) 192, 660, 897, 3324
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 6 cut(s) 417, 2256, 2349, 2638, 2814, 3717
PleI GAGTC 3 cut(s) 442, 3349, 3512
PpsI GAGTC 3 cut(s) 442, 3349, 3512
PscI ACATGT 1 cut(s) 851
PshAI GACNNNNGTC 1 cut(s) 3567
PshBI ATTAAT 1 cut(s) 2246
Psp1406I AACGTT 1 cut(s) 2804
Psp6I CCWGG 4 cut(s) 450, 1915, 3354, 3472
PspGI CCWGG 4 cut(s) 450, 1915, 3354, 3472
PspN4I GGNNCC 1 cut(s) 3230
PspPI GGNCC 4 cut(s) 481, 3461, 3562, 3771
PstI CTGCAG 1 cut(s) 114
PstNI CAGNNNCTG 2 cut(s) 1876, 2353
PvuII CAGCTG 1 cut(s) 1171
RseI CAYNNNNRTG 2 cut(s) 1144, 2415
SapI GCTCTTC 1 cut(s) 718
SatI GCNGC 6 cut(s) 416, 2255, 2348, 2637, 2813, 3716
Sau3AI GATC 8 cut(s) 522, 1114, 1435, 1615, 1750, 2587, 2826, 3832
Sau96I GGNCC 4 cut(s) 481, 3461, 3562, 3771
ScaI AGTACT 2 cut(s) 1543, 1721
SchI GAGTC 3 cut(s) 443, 3350, 3513
ScrFI CCNGG 6 cut(s) 142, 143, 452, 1917, 3356, 3474
SduI GDGCHC 3 cut(s) 2744, 2858, 3233
SexAI ACCWGGT 1 cut(s) 1915
SfaNI GCATC 6 cut(s) 1078, 2122, 2350, 2359, 3079, 3727
SfcI CTRYAG 4 cut(s) 110, 218, 577, 588
Sfr274I CTCGAG 1 cut(s) 3169
SfuI TTCGAA 1 cut(s) 2106
SinI GGWCC 1 cut(s) 3562
SlaI CTCGAG 1 cut(s) 3169
SmaI CCCGGG 1 cut(s) 143
SmiMI CAYNNNNRTG 2 cut(s) 1144, 2415
SmlI CTYRAG 7 cut(s) 500, 1187, 1927, 2186, 2281, 3169, 3410
SmoI CTYRAG 7 cut(s) 500, 1187, 1927, 2186, 2281, 3169, 3410
SpeI ACTAGT 1 cut(s) 562
SphI GCATGC 2 cut(s) 1739, 3551
SsiI CCGC 3 cut(s) 149, 416, 2345
SspI AATATT 4 cut(s) 2119, 2308, 2981, 3481
SspMI CTAG 7 cut(s) 332, 563, 629, 1493, 2604, 2882, 3266
StyD4I CCNGG 6 cut(s) 140, 141, 450, 1915, 3354, 3472
StyI CCWWGG 3 cut(s) 295, 331, 1079
TaiI ACGT 5 cut(s) 473, 1167, 2807, 3381, 3571
TaqI TCGA 7 cut(s) 1438, 1458, 1902, 2106, 2787, 3170, 3330
TatI WGTACW 5 cut(s) 854, 1541, 1719, 2464, 3295
TauI GCSGC 1 cut(s) 418
TscAI CASTG 9 cut(s) 92, 249, 1302, 1372, 1629, 1777, 2874, 3145, 3259
TseFI GTSAC 5 cut(s) 1036, 1698, 2528, 3569, 3583
TseI GCWGC 5 cut(s) 2254, 2347, 2636, 2812, 3715
Tsp45I GTSAC 5 cut(s) 1036, 1698, 2528, 3569, 3583
TspGWI ACGGA 3 cut(s) 32, 311, 2437
TspMI CCCGGG 1 cut(s) 141
TspRI CASTG 9 cut(s) 92, 249, 1302, 1372, 1629, 1777, 2874, 3145, 3259
Van91I CCANNNNNTGG 1 cut(s) 490
Vha464I CTTAAG 1 cut(s) 2281
VpaK11BI GGWCC 1 cut(s) 3562
VspI ATTAAT 1 cut(s) 2246
XapI RAATTY 8 cut(s) 389, 660, 672, 712, 1803, 2165, 2709, 2877
XbaI TCTAGA 1 cut(s) 2881
XceI RCATGY 3 cut(s) 855, 1739, 3551
XhoI CTCGAG 1 cut(s) 3169
XmaI CCCGGG 1 cut(s) 141
XmaJI CCTAGG 1 cut(s) 331
XmiI GTMKAC 2 cut(s) 576, 1354
XmnI GAANNNNTTC 4 cut(s) 192, 660, 897, 3324
XspI CTAG 7 cut(s) 332, 563, 629, 1493, 2604, 2882, 3266
ZraI GACGTC 1 cut(s) 471
ZrmI AGTACT 2 cut(s) 1543, 1721
Zsp2I ATGCAT 5 cut(s) 1093, 1318, 1395, 1760, 2137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.