Rh7AG454300

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003701
Physical Location & Seq
Forward (+)
62345407 .. 62365182
19776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG454300.1

Sequence Viewer

Length: 6939 bp
ATGGAGAATCTTGCTGCAATAGCTGGAAAACTTGCCGAGTACACAGTTGAACCAATTGGCCACCAAGTGGGTTATCTAGCTCACTACAAAAGCAACTATCAAAATCTGCTGACCAAGGTTGAAGACTTGCGTGCTGCCAGAGACGAGGTTCAACATAAGGTTGATCGACTTGAGAGAGGAGGTTTAAGAATTGAGACTGCTCTTCGGAACTGGAAGGCAAGAGTGGATGTAATCATCAGAGAGGCAGAGACATTCCTGAATGACAGCGGGTATGCACAGACCGAGTGTCTTCATGGTTTTTGTCCTCATTTGGTGCTGCGTTATCAACAAAGCAAGAAATCAACGAAATTGGTGGAGGAGATTGATGAACTCTATGGAAAAAAGGAGTTCTCCAACTTTGCATATGATCTTCCACAAGAGGTACGTCTTTTATCTACGGGGCACTACAGTACATTTGATTCAAGGAATTCAACTGTCATGAGAGTCATGGATGAACTGAGAAATCCTAATACAGAAATGATTCTGGTGTGGGGGGTCGGTGGGGTAGGGAAGACCACACTTGTGGAAGAAGTTTTAAGGCAAGCTATAGATGTCAACTTATTTGATGATGTGGTGATGGTGAGAGATGTGAAAGAGAACCCAGACCTTGAAAAAATCCAAAAAGAAATTGCCATAAAATTAGGTATGAGCATTGATGAAAATCTTACTCTAGCTGAAAGAGCGTCCATTCTATGTGATAGGATAAAAGACAAGAGGACTCTTTTAGTTTTAGATGATGTTTGGGAAGAAATTGATCTGGTGGCTGTTGGAATTCCCCGTCTGGTGACTTGTAAAGTAGTGCTGACAAGTAGAAGCCGAAAGGAATTGTCCTTGGATATGCGCACACAAACAGATATTTCACTTGATTTTTTAGGTGTAGAGGAAGCATGGAGTTTATTTGTGGAGATGGCTGGTGGTGTTGTCAAAGATAGCCGCATACGAAGTGTAGCAATTCAAGTAGTCAAAAAATGTGGAGGTTTGCCTGTCTTGATTGTTACAGTTGCAAGAGCTCTAAAAGACAGAAACAATTTGGCAGTATGGAAAGACGCCTTGAGACGACTTGGAGGCTCTGACAACAAAGAATTGACGGAAAAAGCATACTTGTCTCTGGAGTGGAGTTACAATCAATTGGAAGATATAGAGCTTAAGTCTTTGTTCTTGCTTTGTGGGATTATTGTAGTTGAGAACCATATTTATTTGGTAGACTTGTTGAGAAAAAGTGTTGCATCGATGGAAATTCCTTCCAACTTTTTTAGTGAAATGGAGAAATTGAAAGTGCTGGGTTTCACTAAAGTGCGTATGCTGTCACTACCTCCATCTCTTCAGTTCTTGAAAAATGTTCAGACTTTGTGTTTAGAAGAATGCTTGTTGGAAGATGTAGCTCTAGTTGGATATCTAAGCAACTTAGAGATTCTCAGTTTTGCGAGGTCCAACATTAAGCAGCTACCTAAAGAAATAGGGCAGTTGATTCGACTGAGATGGTTGGATTTGAGTGATTGTGCCAAACTTGAAGTGATTTCACCAGATGTTATTTCAAGGTTGGAAAGGCTAGAAGACTTAACAATGAGAAACATATTTCTCAAATGGGAGGCTGAAAGGGGAGGAAGTAATGCTAGTCTTTTAGAGTTGAAAGACTTGTCTCAACTAACTGCATTGGAGATATATATTCGAGATGCTAATATTCTTCCATCAAACTTGTTCTCTAGTAAGTTGGTAAGATACCAAATATATATTGGCAATGTTTGGATTAGCAGATTTAGTCGGGAGAAGACTATGGCTGAGACGACCCTCAACACACTAAAACTGAAGCTCGATCCCAGCAGTGAATTGGACCAAGGTATAAAAATATTGTTGAAGAGATCTGAAGATTTGTCTTTGGATGGAACAGAGAGTGTTAATAGAATTATCCGCCAATTATATGTTGAAGATTTTCAGCAACTGATACATCTGCATCTCCAAAACAATGTTGAGTTCACCCATATCATCTATAGGAAGGTTGTGTTCCCCAATTTAACAACCTTGAGCGTAAGTAGCTGTGATCATCTAAGATTCTTGTTCTCATTTTCTATGGCTAAAAGTCTGGTGGAGCTCAAGCATCTTGAAATATCCACATGTCACTCCATAGAAGAAATTGTATCAACAGGGGAGTATGGTGAAGAAAATATGGATAACATGTTTAAGAAGCTGGAAGATTTGAAGCTCCGTGATCTTCCAAATCTCGTTAGGTTCTGCACAGCAAGTTATATTGAATTTCAGGCCTTGAAGAATTTGGAAATAGTGAAATGTTCTAATATGGGGGCATTTATTACATGCAAAGAAATTGAACAGTGGGACCCGGAAGAGAACCATGATCTCAAGAGTGAGACTGTTGTACGGTACTGCCTCTTTAACGAAAAGGTAGGATTTCCTGGCTTGGAGAGACTGAAAATCAATCAGGCAAGGAAGTTAGAGACAATATGGCACATCCAACTTTCTGGGAGAGACTCTTTTTGCAGACTCAGACAAGTTGAGGTTTCCGAGTGCTACAATCTAATAAATATTTTTCCACCTAGTGAAGTGATTGGGGGTTCTTCCGAAGAAATATGTGTGATTGGGGACCCGCTCTTTCCAAACTTGGAAATTCTTGAGGTGGAGCGGTGTTCCAGGTTAAAGAATCTAGAGTCATCCTCAGTATCTGTCGGGAATATAACAACTTTGAAAGTAATTGGTTGCGAGGGATTGGAATATCTAATGACATATTCAGTGGCTAAAAAGTTCGTGCAACTCGCAACAATGGAAGTGAGGAGTTGTGAAAGAATTGTAGTAATAGTGGGAAGTAAAATAGTTGGAGATGATGCAGCACCAAATGAGATTACTTTCAGCCGCTTGGAACAGTTAAAACTTTCAGATCTACCAAGTCTACGAGGATTTTACTCTTCCAGAAATTGCATTGTCAAACTCCCATCCTCGAGCACCTTAATGGTAGAACTCTGCCCAATCAAGTTGACGATTTCCCCTGATGGGCTCCTCGTGCAGGAGGCAGTATCTGATATAGAACAGAGTTATCATCAGTTGAATGATAAGGAACTTAAGCCATTGTTCTTGCTATGTGGGATTACTATGAGGGGAAAATATATTAGCTTCGAAGACTTGTTGAGGTATGGTATGGGTTTGGGTTTCTTTGGAAAAGACTTCAAAGTGGAAAAAGCAAGAGATGCGTTGCATTCACGGATCAAAAAGCTTAAAGATTTTTGTCTACTGACTGATAGCAGCGATAATAGATATGTAAGAATGCTTGACCGTGTACGTGATGTTGCGAAGCAGATTGCGTTAAGAGATCAACACATCTTATCATTAGCGGAAGATGGTCACCTCCATGAGTTGAAAGAATGGACAGATCAGTATTTCTTAGAGAAGTGCACAATCATGTCTTTCCCCTCTAGCAACAATATCCCAAGTCTTCCTGAAGTTATGCACTGCCCAGCTCTGAAAATGTTTCTTTTGCAAGGTGATCTAGATGATGGCTCGCAGAAAATCTCACCTAGATTTTTTGAAAAGATGAACAACCTCAAAGTCTTGAGTTTGCGATGTATGACTATTCCATCACTTCCTCCATCTTTTCAGTCCCTGAAAAATCTGGATGCCCTATGTTTAGATAAGTGCATATTGGGAGACGTCGCTTTAATTGGGGAGCTAAGTAACTTGGAAATTCTTTCTTTTTCAGAATCCAATATCAAACAATTGCCTGGAGAAGTAGGGAAATTGACTCATCTTAGGTTGTTGGATTTATCTGATTGCTCTAATCTTGAAGTGATTTCACCTGGTGTTATTTCAAGCTTAGGGAGACTGGAAGAGTTGAGAATGAGAAATAGCTTTAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCAAACCTTTCAGAGCTGAAGCATTTGTCTCAGCTATCTGCATTAGAAGTACATATTCGTAATGCTGACATTCTTCCAGCAAACTTGTTCTCCCACAAGTTAGAAAGATTCAATATAATTATTGGGGATGCATATATGTGGGAGAAACGTATGACTGAGACGACCCTCAACGCACTAAAAGTGAAGCTCACTACTGCTAGCATTGATGAATTGGATGAAGGCCTAAAATTTCTTGTGAAAAGAACAGAAGACTTGTCCTTATATGCGGAGGGAATGAATACCATTGTCCATTCACTGAACACAGAAGATTTTGGGCATCTGAAACATCTGCAACTCCAAAGCAACAGTGATTTTAATCATATTATAAACAAGGAGGTTGTATTCCCGAATTTAAGGACCTTGAGAGTACATAGATGTGATCGCCCAAGCTTTTTGTTCTCATTTTCTATGCCTGAAAGTCTTGCGGAACTCCAGCATCTCGAGATATCCACATGTGAAATTATGGAAGAGCGTCTATCATCAACAAGAGAATACGGCCAAGAAATTATGGATTACATGTTTTATAAGCGGGAAGATTTGAAGTTAAATGAATTGCCAAACATCACCAGATTTTGTTCAGGAAGCATAGAAAAATTGGACACAGAAGGTTGGATTAAACTTGGGGAATATAATGCTGCTATGAATAGAAATACGAACGAAATTGAAAAGACTCACTCGGAAGAGAACCTTGATATTACGAGCGAGTTGATTAATATACAATACTTCCTCTTTGACCTAAAGGATGTTTTCCCGAAGTTAACAACCTTGACAGTACATGAGCGTGATCGTCTAAGCTTCTTGTTCACAATTTCTATGGCCAAAAGTCTCGTGGAACTCAAACATCTCGAGATATCCACATGTGAAATCATAGAAGAGATTGTTTCATCAACAAGTGAATACTGTCAAGAAAATATGGAGAACATGTTTCCTAAGCTGGAAGATTTGAAGCTAAATAAACTTCCAAACCTCACCAGATTTTGCTCAGGAAGTTATATTCAACTTCCGTCCTTAGAAAAGTTGGATATACAAGGCTGTACCAAACTGGGCGCATTCATTTCTGATAATATTGCTATGAGTGGTAAAAATACTAGAATCTACAAAGAAATTGAAGAGAAGGACTCCGAAGGGAACAACACGTTGAATACTGTACAATACTTCCTCTTTGACGAAAAGATGGGTTTGCCTAACTTGAAAAGCTTGGTGGTTCACCGATGTGATGGTTTACGTTTCTTATCATCCATGGCAAGAAGTCTTGTACAACTCAAACATCTTGAGATATCTGAATGTCAAATCATGGAAGTAGTGTTGACCGAAGTATACAGTGAAGAGAACAAGGATAGCATGTTTCCTAAGTTAGAACAATTGAAGCTAAATGATCTTCCTTTTCTCGATAGATTCTGCTCTGGAAGTTATATTGAATTTGCATCTTTGGAGGGATTGCATTTAGAGCAGTATACTAAATTGGAGACATTCATCTGTAATCCCTTGAGTAACAGTATTGCAAGCTCCAAAGAAGTAGAAGAAAGGAACAGGGATCAGAACATTGAGACAGTTGTACCATGCCTTCTCTTTGACAAAAAGGTAGGGTTCCCAAAGTTAGAGAGATTGACCATCATTGGCCTACCTAAGCTTAAAACAATTTGGCACACCCAACTTGCTCCAGACTCTTTTTGCGGACTCCGGGACGTTACAGTTCAACACTGCAATAGTCTTAAATACATTTTTCCAGGCTCATTGATAGCCGATGTTTTTAATCAGCAGCTACAAAATTTGACAGTGGAGGAATGTGGAGTGGAGGAAATAATTTCCCAGGGAGGGGGACTGGAAGATCAGTTTGTCTTCCCAAACCTATTAAGTTTGGAATTTAGAAGTCTATTCCAACTTAAGAGATTCTATCCAAGGATGCTTGCTTTCAGTTGGTCACTACTCAAGACATTAGTGTTGCTTGATTGTCCGAAAGTGGATATTTTTGCAGCCGAATTTTCAGGCCTTCAGAAAACACATGACTTGTGCAGTTTGGATACACCTGCAGCTAAACTGCTCACTCCGACTAAACAATCTTCTATTCTAATTAACGAGAATTCATTCCCCAAACTGGAAGATTTGACCCTTGTCCCCATGGATATTTGGTACGGTCGACCCATCTCAGCAGTGTGCTGTGAGAAAGCTGTTTTCGCTGGGATCAGTAATAAACACAGAGATGGGTCCCTCCCATATTTAAGAACGTTGAGGCTGCATGGAATGGATATGCGTCTCTGGAAGAAAACCAAACCTGCTGGCCAAAATTCTCCACGGTTGGAAATTCTAGAGGTGAAGTTTTGTAGATTGAATAATCTAGAGTTGTCTTCCTTATCCTTCCAGAATCTAACAATTTTAGAAGTAACATTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCGAGGTTTAAGGCAACTAAAAAAACTGAAAGTTGATGAATGTAAAAGTATGAAACAAATATTGGCAAGTGAAGGAATTGGGGAAGATGACAGTAATTGTGAGATTGTTTTCAGCAAGTTGCAACATTTGGAACTCAGTGATTTAACAAGTTTGGAACGCTTTTGCTCAAGAAATTGCACTGTGAAGGTCCCAACCTTGGAAACTGTAGAGGTAAATTGGTGCTCGATCAAGTTGAAGGTTTCTTCTTCTGGCATACTAGTAGTAGATTCCAATATGCATCTGGATGTACAAAAGTCACATTCTAGTAAAGAGGCTTTCAATACCACTGAGTCTACTTGTAGCTCGGAAAAAACTCAGAAACAACCTGTCACAAACTCCTCTGAAGAAGCTGAACATATTGAGTTTAATGCACGGGAACTCCATGATGATAGCCTTATTGAACGTTTGAAGGAAGAATTAAGGGCCAGTAAAGCAGACATCAGACGTTTGGAGGAAGAATTAAGGGCCGAAAAAGCAGAGAGGAGAGATCATAGTGCACTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

2312

Amino Acids

264.08

Weight (kDa)

5.54

Isoelectric Point (pI)

46.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 157 - 317 1.9e-25 NB-ARC domain
LRR_14 PF23598 476 - 753 1.7e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 661 - 778 1.8e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 815 - 864 4.9e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 907 - 1004 4.2e-06 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_14 PF23598 1154 - 1372 6.9e-12 Leucine-rich repeat region
LRR_14 PF23598 1235 - 1480 2.2e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 1416 - 1511 6.4e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1569 - 1677 1.1e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1715 - 1813 1.1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1852 - 1919 8.5e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 2098 - 2206 4.8e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4292, 4491
AarI CACCTGC 1 cut(s) 6001
AatII GACGTC 1 cut(s) 3696
Acc16I TGCGCA 1 cut(s) 881
Acc36I ACCTGC 2 cut(s) 6001, 6247
AccB7I CCANNNNNTGG 1 cut(s) 67
AccBSI CCGCTC 2 cut(s) 2641, 2674
AccI GTMKAC 7 cut(s) 1242, 2938, 3274, 5283, 5420, 6103, 6725
AclI AACGTT 2 cut(s) 6191, 6835
AclWI GGATC 4 cut(s) 1847, 3257, 5508, 6155
AcoI YGGCCR 4 cut(s) 58, 4462, 4782, 6244
AcuI CTGAAG 7 cut(s) 1346, 1865, 1923, 3504, 3965, 5942, 6795
AcyI GRCGYC 2 cut(s) 1086, 3693
AdeI CACNNNGTG 3 cut(s) 67, 2591, 3842
AfiI CCNNNNNNNGG 7 cut(s) 67, 3039, 3052, 5781, 5782, 6061, 6589
AflII CTTAAG 3 cut(s) 1184, 3107, 5849
AflIII ACRYGT 7 cut(s) 2150, 2211, 4418, 4482, 4823, 4887, 5100
AhdI GACNNNNNGTC 1 cut(s) 285
AhlI ACTAGT 1 cut(s) 6649
AjnI CCWGG 6 cut(s) 2446, 2681, 3763, 3838, 5692, 5775
AjuI GAANNNNNNNTTGG 2 cut(s) 6437, 6469
AleI CACNNNNGTG 3 cut(s) 560, 1331, 5178
Alw21I GWGCWC 6 cut(s) 1051, 2130, 2993, 3440, 6617, 6931
Alw44I GTGCAC 2 cut(s) 3436, 6927
AlwI GGATC 4 cut(s) 1847, 3257, 5508, 6155
AlwNI CAGNNNCTG 4 cut(s) 1978, 2714, 3065, 3646
Ama87I CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
ApaLI GTGCAC 2 cut(s) 3436, 6927
ArsI GACNNNNNNTTYG 2 cut(s) 6367, 6399
AseI ATTAAT 1 cut(s) 4677
Asp700I GAANNNNTTC 3 cut(s) 519, 1968, 6050
AspLEI GCGC 2 cut(s) 882, 5015
AspS9I GGNCC 9 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580, 6855, 6897
AsuC2I CCSGG 2 cut(s) 2375, 5648
AsuII TTCGAA 1 cut(s) 3162
AsuNHI GCTAGC 1 cut(s) 4124
AvaI CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
AvaII GGWCC 7 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580
BaeGI GKGCMC 3 cut(s) 444, 3440, 6931
BaeI ACNNNNGTAYC 2 cut(s) 5505, 5538
BalI TGGCCA 3 cut(s) 60, 4784, 6246
BanII GRGCYC 3 cut(s) 1051, 2130, 3045
BarI GAAGNNNNNNTAC 2 cut(s) 5514, 5546
BauI CACGAG 2 cut(s) 3047, 4793
Bbv12I GWGCWC 6 cut(s) 1051, 2130, 2993, 3440, 6617, 6931
BceAI ACGGC 1 cut(s) 4477
BcgI CGANNNNNNTGC 2 cut(s) 1490, 1524
BciT130I CCWGG 6 cut(s) 2448, 2683, 3765, 3840, 5694, 5777
BciVI GTATCC 1 cut(s) 5980
BclI TGATCA 1 cut(s) 2077
BcnI CCSGG 2 cut(s) 2375, 5648
BcuI ACTAGT 1 cut(s) 6649
BfmI CTRYAG 5 cut(s) 445, 585, 2026, 5994, 6597
BfrI CTTAAG 3 cut(s) 1184, 3107, 5849
BfuAI ACCTGC 2 cut(s) 6001, 6247
BfuI GTATCC 1 cut(s) 5980
BglII AGATCT 2 cut(s) 1898, 2926
Bme1390I CCNGG 8 cut(s) 2375, 2448, 2683, 3765, 3840, 5648, 5694, 5777
Bme18I GGWCC 7 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580
BmeRI GACNNNNNGTC 1 cut(s) 285
BmeT110I CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
BmgT120I GGNCC 9 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580, 6855, 6897
BmiI GGNNCC 9 cut(s) 2372, 2373, 2636, 2637, 3044, 5555, 6172, 6173, 6582
BmrFI CCNGG 8 cut(s) 2375, 2448, 2683, 3765, 3840, 5648, 5694, 5777
BmrI ACTGGG 1 cut(s) 5018
BmtI GCTAGC 1 cut(s) 4128
BmuI ACTGGG 1 cut(s) 5018
BoxI GACNNNNGTC 1 cut(s) 6077
BplI GAGNNNNNCTC 4 cut(s) 2690, 2722, 5069, 5101
BpmI CTGGAG 4 cut(s) 1169, 3786, 4382, 5610
Bpu10I CCTNAGC 4 cut(s) 3856, 4896, 4948, 5592
Bpu14I TTCGAA 1 cut(s) 3162
BpuMI CCSGG 2 cut(s) 2375, 5648
Bsa29I ATCGAT 1 cut(s) 1268
BsaAI YACGTR 1 cut(s) 3326
BsaBI GATNNNNATC 2 cut(s) 699, 4281
BsaHI GRCGYC 2 cut(s) 1086, 3693
BsaXI ACNNNNNCTCC 6 cut(s) 4001, 4031, 4874, 4904, 6795, 6825
Bsc4I CCNNNNNNNGG 7 cut(s) 67, 3039, 3052, 5781, 5782, 6061, 6589
Bse1I ACTGG 6 cut(s) 215, 3870, 5013, 5793, 6066, 6858
Bse3DI GCAATG 1 cut(s) 1783
Bse8I GATNNNNATC 2 cut(s) 699, 4281
BseBI CCWGG 6 cut(s) 2448, 2683, 3765, 3840, 5694, 5777
BseCI ATCGAT 1 cut(s) 1268
BseJI GATNNNNATC 2 cut(s) 699, 4281
BseLI CCNNNNNNNGG 7 cut(s) 67, 3039, 3052, 5781, 5782, 6061, 6589
BseMI GCAATG 1 cut(s) 1783
BseNI ACTGG 6 cut(s) 215, 3870, 5013, 5793, 6066, 6858
BseRI GAGGAG 6 cut(s) 192, 371, 2836, 3035, 6760, 6928
BseSI GKGCMC 3 cut(s) 444, 3440, 6931
BseYI CCCAGC 4 cut(s) 1318, 1856, 3499, 6143
BsgI GTGCAG 3 cut(s) 2254, 3071, 5998
Bsh1285I CGRYCG 1 cut(s) 6103
BshVI ATCGAT 1 cut(s) 1268
BsiEI CGRYCG 1 cut(s) 6103
BsiHKAI GWGCWC 6 cut(s) 1051, 2130, 2993, 3440, 6617, 6931
BsiHKCI CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
BsiSI CCGG 2 cut(s) 2375, 5647
BslFI GGGAC 8 cut(s) 2384, 2648, 3628, 5663, 5799, 6065, 6157, 6566
BslI CCNNNNNNNGG 7 cut(s) 67, 3039, 3052, 5781, 5782, 6061, 6589
BsmBI CGTCTC 6 cut(s) 135, 1087, 1814, 3684, 4079, 6224
BsmFI GGGAC 8 cut(s) 2384, 2648, 3628, 5663, 5799, 6065, 6157, 6566
BsmI GAATGC 4 cut(s) 1406, 3241, 3315, 5015
BsoBI CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
Bsp119I TTCGAA 1 cut(s) 3162
Bsp1286I GDGCHC 8 cut(s) 444, 1051, 2130, 2993, 3045, 3440, 6617, 6931
Bsp1407I TGTACA 3 cut(s) 5113, 5221, 6679
Bsp19I CCATGG 2 cut(s) 5205, 6084
BspDI ATCGAT 1 cut(s) 1268
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 9 cut(s) 2372, 2373, 2636, 2637, 3044, 5555, 6172, 6173, 6582
BspMAI CTGCAG 1 cut(s) 5998
BspMI ACCTGC 2 cut(s) 6001, 6247
BspOI GCTAGC 1 cut(s) 4128
BspPI GGATC 4 cut(s) 1847, 3257, 5508, 6155
BspQI GCTCTTC 2 cut(s) 207, 4428
BspT104I TTCGAA 1 cut(s) 3162
BspTI CTTAAG 3 cut(s) 1184, 3107, 5849
BsrBI CCGCTC 2 cut(s) 2641, 2674
BsrDI GCAATG 1 cut(s) 1783
BsrGI TGTACA 3 cut(s) 5113, 5221, 6679
BsrI ACTGG 6 cut(s) 215, 3870, 5013, 5793, 6066, 6858
BssNAI GTATAC 2 cut(s) 5284, 5421
BssNI GRCGYC 2 cut(s) 1086, 3693
BssSI CACGAG 2 cut(s) 3047, 4793
BssT1I CCWWGG 7 cut(s) 114, 870, 1873, 5205, 5864, 6084, 6588
Bst1107I GTATAC 2 cut(s) 5284, 5421
Bst2BI CACGAG 2 cut(s) 3047, 4793
Bst2UI CCWGG 6 cut(s) 2448, 2683, 3765, 3840, 5694, 5777
BstACI GRCGYC 2 cut(s) 1086, 3693
BstAFI CTTAAG 3 cut(s) 1184, 3107, 5849
BstAPI GCANNNNNTGC 1 cut(s) 3233
BstAUI TGTACA 3 cut(s) 5113, 5221, 6679
BstBAI YACGTR 1 cut(s) 3326
BstBI TTCGAA 1 cut(s) 3162
BstC8I GCNNGC 8 cut(s) 132, 582, 3545, 4126, 5470, 5874, 6244, 6359
BstDSI CCRYGG 3 cut(s) 5205, 6084, 6257
BstEII GGTNACC 1 cut(s) 3386
BstENI CCTNNNNNAGG 1 cut(s) 3050
BstHHI GCGC 2 cut(s) 882, 5015
BstMCI CGRYCG 1 cut(s) 6103
BstMWI GCNNNNNNNGC 9 cut(s) 20, 719, 2070, 3049, 3233, 5413, 6140, 6863, 6905
BstNI CCWGG 6 cut(s) 2448, 2683, 3765, 3840, 5694, 5777
BstNSI RCATGY 8 cut(s) 2154, 2215, 2352, 4422, 4486, 4827, 4891, 5311
BstPAI GACNNNNGTC 1 cut(s) 6077
BstPI GGTNACC 1 cut(s) 3386
BstSCI CCNGG 8 cut(s) 2373, 2446, 2681, 3763, 3838, 5646, 5692, 5775
BstSFI CTRYAG 5 cut(s) 445, 585, 2026, 5994, 6597
BstSLI GKGCMC 3 cut(s) 444, 3440, 6931
BstX2I RGATCY 2 cut(s) 1898, 2926
BstXI CCANNNNNNTGG 2 cut(s) 562, 2513
BstYI RGATCY 2 cut(s) 1898, 2926
BstZ17I GTATAC 2 cut(s) 5284, 5421
Bsu15I ATCGAT 1 cut(s) 1268
BsuI GTATCC 1 cut(s) 5980
BsuTUI ATCGAT 1 cut(s) 1268
BtgI CCRYGG 3 cut(s) 5205, 6084, 6257
BtgZI GCGATG 1 cut(s) 3619
BtsI GCAGTG 4 cut(s) 1867, 3493, 5665, 6123
BveI ACCTGC 2 cut(s) 6001, 6247
Cac8I GCNNGC 8 cut(s) 132, 582, 3545, 4126, 5470, 5874, 6244, 6359
CaiI CAGNNNCTG 4 cut(s) 1978, 2714, 3065, 3646
CciI TCATGA 1 cut(s) 477
CfoI GCGC 2 cut(s) 882, 5015
Cfr13I GGNCC 9 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580, 6855, 6897
ClaI ATCGAT 1 cut(s) 1268
CseI GACGC 4 cut(s) 711, 1094, 4427, 6206
CsiI ACCWGGT 1 cut(s) 3838
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
DraIII CACNNNGTG 3 cut(s) 67, 2591, 3842
DriI GACNNNNNGTC 1 cut(s) 285
EaeI YGGCCR 4 cut(s) 58, 4462, 4782, 6244
Eam1105I GACNNNNNGTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 1937
Ecl136II GAGCTC 2 cut(s) 1049, 2128
Eco130I CCWWGG 7 cut(s) 114, 870, 1873, 5205, 5864, 6084, 6588
Eco147I AGGCCT 3 cut(s) 2297, 4149, 5955
Eco24I GRGCYC 3 cut(s) 1051, 2130, 3045
Eco32I GATATC 4 cut(s) 1433, 4413, 4818, 5244
Eco47I GGWCC 7 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580
Eco53kI GAGCTC 2 cut(s) 1049, 2128
Eco57I CTGAAG 7 cut(s) 1346, 1865, 1923, 3504, 3965, 5942, 6795
Eco88I CYCGRG 4 cut(s) 2986, 4406, 4811, 6393
Eco91I GGTNACC 1 cut(s) 3386
EcoICRI GAGCTC 2 cut(s) 1049, 2128
EcoNI CCTNNNNNAGG 1 cut(s) 3050
EcoO109I RGGNCCY 5 cut(s) 2371, 2635, 4323, 6171, 6580
EcoO65I GGTNACC 1 cut(s) 3386
EcoRI GAATTC 3 cut(s) 466, 810, 6046
EcoRII CCWGG 6 cut(s) 2446, 2681, 3763, 3838, 5692, 5775
EcoRV GATATC 4 cut(s) 1433, 4413, 4818, 5244
EcoT14I CCWWGG 7 cut(s) 114, 870, 1873, 5205, 5864, 6084, 6588
EcoT22I ATGCAT 2 cut(s) 4060, 6672
EcoT38I GRGCYC 3 cut(s) 1051, 2130, 3045
ErhI CCWWGG 7 cut(s) 114, 870, 1873, 5205, 5864, 6084, 6588
Esp3I CGTCTC 6 cut(s) 135, 1087, 1814, 3684, 4079, 6224
FalI AAGNNNNNCTT 2 cut(s) 4638, 4670
FaqI GGGAC 8 cut(s) 2384, 2648, 3628, 5663, 5799, 6065, 6157, 6566
FauI CCCGC 3 cut(s) 260, 2646, 4488
FauNDI CATATG 1 cut(s) 403
FbaI TGATCA 1 cut(s) 2077
FblI GTMKAC 7 cut(s) 1242, 2938, 3274, 5283, 5420, 6103, 6725
FriOI GRGCYC 3 cut(s) 1051, 2130, 3045
FspAI RTGCGCAY 1 cut(s) 881
FspI TGCGCA 1 cut(s) 881
GlaI GCGC 2 cut(s) 881, 5014
GsaI CCCAGC 4 cut(s) 1322, 1860, 3503, 6147
GsuI CTGGAG 4 cut(s) 1169, 3786, 4382, 5610
HapII CCGG 2 cut(s) 2375, 5647
HgaI GACGC 4 cut(s) 711, 1094, 4427, 6206
HhaI GCGC 2 cut(s) 882, 5015
Hin1I GRCGYC 2 cut(s) 1086, 3693
Hin6I GCGC 2 cut(s) 880, 5013
HinP1I GCGC 2 cut(s) 880, 5013
HincII GTYRAC 5 cut(s) 595, 3024, 4725, 5274, 6104
HindII GTYRAC 5 cut(s) 595, 3024, 4725, 5274, 6104
HindIII AAGCTT 6 cut(s) 3257, 3853, 4354, 4759, 5161, 5594
HpaI GTTAAC 1 cut(s) 4725
HpaII CCGG 2 cut(s) 2375, 5647
Hpy99I CGWCG 1 cut(s) 3698
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 719, 2070, 3049, 3233, 5413, 6140, 6863, 6905
Hsp92I GRCGYC 2 cut(s) 1086, 3693
HspAI GCGC 2 cut(s) 880, 5013
KflI GGGWCCC 3 cut(s) 2371, 2635, 6171
Ksp22I TGATCA 1 cut(s) 2077
KspAI GTTAAC 1 cut(s) 4725
LguI GCTCTTC 2 cut(s) 207, 4428
LmnI GCTCC 7 cut(s) 2125, 2244, 2671, 3048, 3709, 5477, 5629
MabI ACCWGGT 1 cut(s) 3838
MbiI CCGCTC 2 cut(s) 2641, 2674
MfeI CAATTG 4 cut(s) 54, 1166, 3758, 5327
MflI RGATCY 2 cut(s) 1898, 2926
MhlI GDGCHC 8 cut(s) 444, 1051, 2130, 2993, 3045, 3440, 6617, 6931
MlsI TGGCCA 3 cut(s) 60, 4784, 6246
MluNI TGGCCA 3 cut(s) 60, 4784, 6246
Mox20I TGGCCA 3 cut(s) 60, 4784, 6246
Mph1103I ATGCAT 2 cut(s) 4060, 6672
MroXI GAANNNNTTC 3 cut(s) 519, 1968, 6050
MscI TGGCCA 3 cut(s) 60, 4784, 6246
Msp20I TGGCCA 3 cut(s) 60, 4784, 6246
MspA1I CMGCKG 1 cut(s) 267
MspCI CTTAAG 3 cut(s) 1184, 3107, 5849
MspI CCGG 2 cut(s) 2375, 5647
MspR9I CCNGG 8 cut(s) 2375, 2448, 2683, 3765, 3840, 5648, 5694, 5777
MunI CAATTG 4 cut(s) 54, 1166, 3758, 5327
Mva1269I GAATGC 4 cut(s) 1406, 3241, 3315, 5015
MvaI CCWGG 6 cut(s) 2448, 2683, 3765, 3840, 5694, 5777
MwoI GCNNNNNNNGC 9 cut(s) 20, 719, 2070, 3049, 3233, 5413, 6140, 6863, 6905
NciI CCSGG 2 cut(s) 2375, 5648
NcoI CCATGG 2 cut(s) 5205, 6084
NdeI CATATG 1 cut(s) 403
NheI GCTAGC 1 cut(s) 4124
NlaIV GGNNCC 9 cut(s) 2372, 2373, 2636, 2637, 3044, 5555, 6172, 6173, 6582
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 7 cut(s) 823, 1344, 2153, 3386, 5886, 6687, 6760
NsbI TGCGCA 1 cut(s) 881
NsiI ATGCAT 2 cut(s) 4060, 6672
NspI RCATGY 8 cut(s) 2154, 2215, 2352, 4422, 4486, 4827, 4891, 5311
NspV TTCGAA 1 cut(s) 3162
OliI CACNNNNGTG 3 cut(s) 560, 1331, 5178
PaeR7I CTCGAG 4 cut(s) 2986, 4406, 4811, 6393
PagI TCATGA 1 cut(s) 477
PaqCI CACCTGC 1 cut(s) 6001
PasI CCCWGGG 1 cut(s) 5776
PceI AGGCCT 3 cut(s) 2297, 4149, 5955
PciI ACATGT 6 cut(s) 2150, 2211, 4418, 4482, 4823, 4887
PciSI GCTCTTC 2 cut(s) 207, 4428
PctI GAATGC 4 cut(s) 1406, 3241, 3315, 5015
PdmI GAANNNNTTC 3 cut(s) 519, 1968, 6050
PflMI CCANNNNNTGG 1 cut(s) 67
PfoI TCCNGGA 1 cut(s) 5646
Ppu21I YACGTR 1 cut(s) 3326
PpuMI RGGWCCY 5 cut(s) 2371, 2635, 4323, 6171, 6580
PscI ACATGT 6 cut(s) 2150, 2211, 4418, 4482, 4823, 4887
PshAI GACNNNNGTC 1 cut(s) 6077
PshBI ATTAAT 1 cut(s) 4677
PsiI TTATAA 2 cut(s) 4292, 4491
Psp124BI GAGCTC 2 cut(s) 1051, 2130
Psp1406I AACGTT 2 cut(s) 6191, 6835
Psp5II RGGWCCY 5 cut(s) 2371, 2635, 4323, 6171, 6580
Psp6I CCWGG 6 cut(s) 2446, 2681, 3763, 3838, 5692, 5775
PspEI GGTNACC 1 cut(s) 3386
PspFI CCCAGC 4 cut(s) 1318, 1856, 3499, 6143
PspGI CCWGG 6 cut(s) 2446, 2681, 3763, 3838, 5692, 5775
PspN4I GGNNCC 9 cut(s) 2372, 2373, 2636, 2637, 3044, 5555, 6172, 6173, 6582
PspPI GGNCC 9 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580, 6855, 6897
PspPPI RGGWCCY 5 cut(s) 2371, 2635, 4323, 6171, 6580
PspXI VCTCGAGB 2 cut(s) 2986, 6393
PstI CTGCAG 1 cut(s) 5998
PstNI CAGNNNCTG 4 cut(s) 1978, 2714, 3065, 3646
PsuI RGATCY 2 cut(s) 1898, 2926
SacI GAGCTC 2 cut(s) 1051, 2130
SalI GTCGAC 1 cut(s) 6102
SapI GCTCTTC 2 cut(s) 207, 4428
Sau96I GGNCC 9 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580, 6855, 6897
ScrFI CCNGG 8 cut(s) 2375, 2448, 2683, 3765, 3840, 5648, 5694, 5777
SduI GDGCHC 8 cut(s) 444, 1051, 2130, 2993, 3045, 3440, 6617, 6931
SexAI ACCWGGT 1 cut(s) 3838
SfcI CTRYAG 5 cut(s) 445, 585, 2026, 5994, 6597
Sfr274I CTCGAG 4 cut(s) 2986, 4406, 4811, 6393
SfuI TTCGAA 1 cut(s) 3162
SinI GGWCC 7 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580
SlaI CTCGAG 4 cut(s) 2986, 4406, 4811, 6393
SpeI ACTAGT 1 cut(s) 6649
SseBI AGGCCT 3 cut(s) 2297, 4149, 5955
SspI AATATT 6 cut(s) 1720, 1887, 2578, 5032, 6371, 6453
SstI GAGCTC 2 cut(s) 1051, 2130
StuI AGGCCT 3 cut(s) 2297, 4149, 5955
StyD4I CCNGG 8 cut(s) 2373, 2446, 2681, 3763, 3838, 5646, 5692, 5775
StyI CCWWGG 7 cut(s) 114, 870, 1873, 5205, 5864, 6084, 6588
TaqII GACCGA 2 cut(s) 296, 5291
TatI WGTACW 8 cut(s) 39, 449, 3976, 4333, 4738, 5113, 5221, 6679
TauI GCSGC 2 cut(s) 975, 2904
TseFI GTSAC 7 cut(s) 823, 1344, 2153, 3386, 5886, 6687, 6760
Tsp45I GTSAC 7 cut(s) 823, 1344, 2153, 3386, 5886, 6687, 6760
TspGWI ACGGA 4 cut(s) 1142, 2231, 3262, 4959
Van91I CCANNNNNTGG 1 cut(s) 67
Vha464I CTTAAG 3 cut(s) 1184, 3107, 5849
VneI GTGCAC 2 cut(s) 3436, 6927
VpaK11BI GGWCC 7 cut(s) 1467, 1870, 2371, 2635, 4323, 6171, 6580
VspI ATTAAT 1 cut(s) 4677
XagI CCTNNNNNAGG 1 cut(s) 3050
XbaI TCTAGA 4 cut(s) 2695, 3532, 6271, 6301
XceI RCATGY 8 cut(s) 2154, 2215, 2352, 4422, 4486, 4827, 4891, 5311
XcmI CCANNNNNNNNNTGG 4 cut(s) 1769, 1864, 4792, 6670
XhoI CTCGAG 4 cut(s) 2986, 4406, 4811, 6393
XmiI GTMKAC 7 cut(s) 1242, 2938, 3274, 5283, 5420, 6103, 6725
XmnI GAANNNNTTC 3 cut(s) 519, 1968, 6050
ZraI GACGTC 1 cut(s) 3694
Zsp2I ATGCAT 2 cut(s) 4060, 6672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.