RchiOBHm_Chr7g0203351

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003701
Physical Location & Seq
Forward (+)
21056862 .. 21058732
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18200

Sequence Viewer

Length: 1314 bp
ATGCAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATATGGAACTGTGATCATCTAAGTTTCTTGTTCTCATTTTCTATGGCTCAAAGTCTGGTAGAGCTCAAACATCTTGAAGTATCCAGATGTGCAATAATGGAAGTGATAATATCATCAAGAGATCAACCTAATGGAGAAAATATCGATATCGTGTTCCGTAAGTTGGAAAGTCTAACATTAAAGCAACTTCCAAACCTCGATAGATTTTGTACTGAAAGTTGTACTGAATTTTCTTCCGTGATGAAATTTGATGCCGAACTGGGGGCATCCATCTTTGATCCTACGAGTAGTAAAAAACTTGATGTCAAGAATGGGACTGCTATACGAGCATGTCTTTTTGACCCACAGGTTGTGTTCCCCAACTTAACAACCTTGAGCATATGGAACTGTGATCATCTAAGTTTCTTGTTCTCATTTTCTATGGCTCAAAGTCTGGTGGAGCTCAAACATCTTGAAGTATCCGGATGTGCAATAATGGAAGCAATAATATCATCAAGAGATCAACCTAATGGAGAAAATATCGATATCGTGTTCCGTAAGTTGGAAAGTCTAACATTAAAGCAACTTCCAAACCTCGATAGATTCTGTACTGAAAGTTGTACTGAATTTTCTTCCGTGATGAAATTTGATGCCGAGCTGGGGGCATCCATCTTTGATCCTATGAGTAGTAAAAAACTTGATGTCGAGAATGGGACTGCTATACGAGCATGTCTTTTTGACCCACAGGTAAAATTTCCGAGCTTGGAGAGACTGAAAATCGATGGGCTAGGGAAGTTGCACACGCTATGGAACAATAAATTTGCTGATCAAGACTCTTTTTGCCAACTCAAAGAAGTTGAAGTTTCAAATTGCAACAGTCTGAAAAAGATCTTTCCAGTCTCAGTAGCCAGACGTCTCCAAAAGCTACATTCGCTGTATGTGATAGGGTGTGATGAAGTGGAGGAAATCATTACTGGTGAACAAGGACTAGAAACAGAGTCTCCTCATTTAGTGTTTCCAAAAGTAACACATGTGGGATTTCTCTATCTTCCCAAACTGAGTAGATTCTATCCTGGGATTATTGCTTCCAAGTGGCCGTTACTCAAACAACTAAACATTTATGGATGTGGGAAAATGAAGATGTTTGCCGCAGAACTTCCAATTTCTTCAGAAAGACATGAGCCGAACCCTCTAATTGAACAATCTCTCTTTCTAATTGACAAGGTACACAGTTTTCATAGACGAAATTTCTCCGTGAATATTTTCATTGTGAATTTATGTGCTAAACTTTTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

437

Amino Acids

49.45

Weight (kDa)

6.04

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 4 - 89 1.1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 127 - 215 6.6e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 257 - 323 1.9e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 931
AccIII TCCGGA 1 cut(s) 497
AciI CCGC 1 cut(s) 1164
AclWI GGATC 2 cut(s) 308, 686
AcoI YGGCCR 1 cut(s) 1109
AcsI RAATTY 8 cut(s) 263, 281, 641, 659, 767, 833, 1261, 1288
AcuI CTGAAG 1 cut(s) 1167
AcyI GRCGYC 1 cut(s) 928
AfaI GTAC 5 cut(s) 247, 259, 625, 637, 1242
AfiI CCNNNNNNNGG 4 cut(s) 199, 297, 577, 675
AflIII ACRYGT 1 cut(s) 1045
AgsI TTSAA 5 cut(s) 113, 491, 875, 882, 1214
AjnI CCWGG 1 cut(s) 1087
AluBI AGCT 5 cut(s) 100, 478, 673, 777, 940
AluI AGCT 5 cut(s) 100, 478, 673, 777, 940
Alw21I GWGCWC 2 cut(s) 102, 480
Alw26I GTCTC 4 cut(s) 778, 919, 935, 1020
AlwI GGATC 2 cut(s) 308, 686
Aor13HI TCCGGA 1 cut(s) 497
AoxI GGCC 1 cut(s) 1109
ApoI RAATTY 8 cut(s) 263, 281, 641, 659, 767, 833, 1261, 1288
Asp700I GAANNNNTTC 1 cut(s) 1277
AsuHPI GGTGA 1 cut(s) 1004
BanII GRGCYC 2 cut(s) 102, 480
Bbv12I GWGCWC 2 cut(s) 102, 480
BccI CCATC 3 cut(s) 314, 692, 791
BceAI ACGGC 1 cut(s) 1096
BciT130I CCWGG 1 cut(s) 1089
BciVI GTATCC 2 cut(s) 127, 505
BclI TGATCA 3 cut(s) 49, 427, 841
BcoDI GTCTC 4 cut(s) 778, 919, 935, 1020
BfaI CTAG 2 cut(s) 803, 1004
BfuI GTATCC 2 cut(s) 127, 505
BglII AGATCT 1 cut(s) 903
BisI GCNGC 1 cut(s) 1164
BlsI GCNGC 1 cut(s) 1165
Bme1390I CCNGG 1 cut(s) 1089
BmrFI CCNGG 1 cut(s) 1089
BmrI ACTGGG 1 cut(s) 305
BmsI GCATC 4 cut(s) 277, 311, 655, 689
BmuI ACTGGG 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 52, 430
Bsa29I ATCGAT 3 cut(s) 180, 558, 795
BsaHI GRCGYC 1 cut(s) 928
BsaJI CCNNGG 1 cut(s) 1088
BsaWI WCCGGW 1 cut(s) 497
BsaXI ACNNNNNCTCC 2 cut(s) 1000, 1030
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 297, 577, 675
Bse1I ACTGG 3 cut(s) 300, 911, 994
BseAI TCCGGA 1 cut(s) 497
BseBI CCWGG 1 cut(s) 1089
BseCI ATCGAT 3 cut(s) 180, 558, 795
BseDI CCNNGG 1 cut(s) 1088
BseGI GGATG 4 cut(s) 302, 506, 680, 1145
BseLI CCNNNNNNNGG 4 cut(s) 199, 297, 577, 675
BseMII CTCAG 2 cut(s) 930, 1064
BseNI ACTGG 3 cut(s) 300, 911, 994
BseRI GAGGAG 1 cut(s) 1008
BseYI CCCAGC 1 cut(s) 673
BshFI GGCC 1 cut(s) 1111
BshVI ATCGAT 3 cut(s) 180, 558, 795
BsiHKAI GWGCWC 2 cut(s) 102, 480
BsiSI CCGG 1 cut(s) 498
BslFI GGGAC 2 cut(s) 364, 742
BslI CCNNNNNNNGG 4 cut(s) 199, 297, 577, 675
BsmAI GTCTC 4 cut(s) 778, 919, 935, 1020
BsmBI CGTCTC 1 cut(s) 935
BsmFI GGGAC 2 cut(s) 364, 742
BsnI GGCC 1 cut(s) 1111
Bsp1286I GDGCHC 2 cut(s) 102, 480
Bsp13I TCCGGA 1 cut(s) 497
Bsp143I GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
BspACI CCGC 1 cut(s) 1164
BspANI GGCC 1 cut(s) 1111
BspCNI CTCAG 2 cut(s) 929, 1065
BspDI ATCGAT 3 cut(s) 180, 558, 795
BspEI TCCGGA 1 cut(s) 497
BspPI GGATC 2 cut(s) 308, 686
BsrI ACTGG 3 cut(s) 300, 911, 994
BssECI CCNNGG 1 cut(s) 1088
BssMI GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
BssNI GRCGYC 1 cut(s) 928
Bst2UI CCWGG 1 cut(s) 1089
Bst4CI ACNGT 4 cut(s) 47, 425, 893, 1247
BstACI GRCGYC 1 cut(s) 928
BstDEI CTNAG 4 cut(s) 56, 434, 916, 1073
BstF5I GGATG 4 cut(s) 302, 506, 680, 1145
BstKTI GATC 8 cut(s) 52, 160, 316, 430, 538, 694, 844, 906
BstMAI GTCTC 4 cut(s) 778, 919, 935, 1020
BstMBI GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
BstMWI GCNNNNNNNGC 3 cut(s) 362, 740, 946
BstNI CCWGG 1 cut(s) 1089
BstNSI RCATGY 3 cut(s) 369, 747, 1049
BstSCI CCNGG 1 cut(s) 1087
BstX2I RGATCY 1 cut(s) 903
BstYI RGATCY 1 cut(s) 903
Bsu15I ATCGAT 3 cut(s) 180, 558, 795
BsuI GTATCC 2 cut(s) 127, 505
BsuRI GGCC 1 cut(s) 1111
BsuTUI ATCGAT 3 cut(s) 180, 558, 795
BtsCI GGATG 4 cut(s) 302, 506, 680, 1145
ClaI ATCGAT 3 cut(s) 180, 558, 795
Csp6I GTAC 5 cut(s) 246, 258, 624, 636, 1241
CviAII CATG 4 cut(s) 366, 744, 1046, 1193
CviQI GTAC 5 cut(s) 246, 258, 624, 636, 1241
DdeI CTNAG 4 cut(s) 56, 434, 916, 1073
DpnI GATC 8 cut(s) 51, 159, 315, 429, 537, 693, 843, 905
DpnII GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
EaeI YGGCCR 1 cut(s) 1109
Ecl136II GAGCTC 2 cut(s) 100, 478
Eco24I GRGCYC 2 cut(s) 102, 480
Eco32I GATATC 2 cut(s) 184, 562
Eco53kI GAGCTC 2 cut(s) 100, 478
Eco57I CTGAAG 1 cut(s) 1167
EcoICRI GAGCTC 2 cut(s) 100, 478
EcoRII CCWGG 1 cut(s) 1087
EcoRV GATATC 2 cut(s) 184, 562
EcoT38I GRGCYC 2 cut(s) 102, 480
Esp3I CGTCTC 1 cut(s) 935
FaeI CATG 4 cut(s) 369, 747, 1049, 1196
FaqI GGGAC 2 cut(s) 364, 742
FatI CATG 4 cut(s) 365, 743, 1045, 1192
FauNDI CATATG 2 cut(s) 38, 416
FbaI TGATCA 3 cut(s) 49, 427, 841
Fnu4HI GCNGC 1 cut(s) 1164
FokI GGATG 4 cut(s) 289, 513, 667, 1152
FriOI GRGCYC 2 cut(s) 102, 480
Fsp4HI GCNGC 1 cut(s) 1164
FspBI CTAG 2 cut(s) 803, 1004
GluI GCNGC 1 cut(s) 1164
GsaI CCCAGC 1 cut(s) 677
HaeIII GGCC 1 cut(s) 1111
HapII CCGG 1 cut(s) 498
Hin1I GRCGYC 1 cut(s) 928
Hin1II CATG 4 cut(s) 369, 747, 1049, 1196
HinfI GANTC 4 cut(s) 618, 848, 1013, 1080
HpaII CCGG 1 cut(s) 498
HphI GGTGA 1 cut(s) 1004
Hpy166II GTNNAC 2 cut(s) 995, 1243
Hpy188I TCNGA 3 cut(s) 774, 897, 1186
Hpy188III TCNNGA 9 cut(s) 110, 120, 153, 343, 488, 498, 531, 721, 845
Hpy8I GTNNAC 2 cut(s) 995, 1243
HpyCH4III ACNGT 4 cut(s) 47, 425, 893, 1247
HpyCH4IV ACGT 1 cut(s) 928
HpyCH4V TGCA 5 cut(s) 4, 128, 506, 814, 888
HpyF10VI GCNNNNNNNGC 3 cut(s) 362, 740, 946
HpyF3I CTNAG 4 cut(s) 56, 434, 916, 1073
HpySE526I ACGT 1 cut(s) 928
Hsp92I GRCGYC 1 cut(s) 928
Hsp92II CATG 4 cut(s) 369, 747, 1049, 1196
Kpn2I TCCGGA 1 cut(s) 497
Ksp22I TGATCA 3 cut(s) 49, 427, 841
Kzo9I GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
LmnI GCTCC 1 cut(s) 475
LweI GCATC 4 cut(s) 277, 311, 655, 689
MaeI CTAG 2 cut(s) 803, 1004
MaeII ACGT 1 cut(s) 928
MaeIII GTNAC 2 cut(s) 1039, 1113
MalI GATC 8 cut(s) 51, 159, 315, 429, 537, 693, 843, 905
MboI GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
MboII GAAGA 5 cut(s) 261, 639, 1055, 1165, 1173
MflI RGATCY 1 cut(s) 903
MhlI GDGCHC 2 cut(s) 102, 480
MlyI GAGTC 2 cut(s) 842, 1022
MmeI TCCRAC 2 cut(s) 180, 558
MnlI CCTC 5 cut(s) 242, 620, 970, 1029, 1215
MroI TCCGGA 1 cut(s) 497
MroXI GAANNNNTTC 1 cut(s) 1277
MseI TTAA 5 cut(s) 23, 215, 401, 593, 1312
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 1 cut(s) 1089
MvaI CCWGG 1 cut(s) 1089
MwoI GCNNNNNNNGC 3 cut(s) 362, 740, 946
NdeI CATATG 2 cut(s) 38, 416
NdeII GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
NlaIII CATG 4 cut(s) 369, 747, 1049, 1196
NmeAIII GCCGAG 1 cut(s) 694
NspI RCATGY 3 cut(s) 369, 747, 1049
PciI ACATGT 1 cut(s) 1045
PdmI GAANNNNTTC 1 cut(s) 1277
PfeI GAWTC 2 cut(s) 618, 1080
PkrI GCNGC 1 cut(s) 1165
PleI GAGTC 2 cut(s) 842, 1021
PpsI GAGTC 2 cut(s) 842, 1021
PscI ACATGT 1 cut(s) 1045
Psp124BI GAGCTC 2 cut(s) 102, 480
Psp6I CCWGG 1 cut(s) 1087
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 1 cut(s) 1087
PsuI RGATCY 1 cut(s) 903
RsaI GTAC 5 cut(s) 247, 259, 625, 637, 1242
RsaNI GTAC 5 cut(s) 246, 258, 624, 636, 1241
SacI GAGCTC 2 cut(s) 102, 480
SaqAI TTAA 5 cut(s) 23, 215, 401, 593, 1312
SatI GCNGC 1 cut(s) 1164
Sau3AI GATC 8 cut(s) 49, 157, 313, 427, 535, 691, 841, 903
SchI GAGTC 2 cut(s) 842, 1022
ScrFI CCNGG 1 cut(s) 1089
SduI GDGCHC 2 cut(s) 102, 480
SfaNI GCATC 4 cut(s) 277, 311, 655, 689
SmlI CTYRAG 2 cut(s) 31, 409
SmoI CTYRAG 2 cut(s) 31, 409
SsiI CCGC 1 cut(s) 1164
SspI AATATT 1 cut(s) 1276
SspMI CTAG 2 cut(s) 803, 1004
SstI GAGCTC 2 cut(s) 102, 480
StyD4I CCNGG 1 cut(s) 1087
TaaI ACNGT 4 cut(s) 47, 425, 893, 1247
TaiI ACGT 1 cut(s) 931
TaqI TCGA 6 cut(s) 180, 234, 558, 612, 720, 795
TatI WGTACW 4 cut(s) 245, 257, 623, 635
TauI GCSGC 1 cut(s) 1166
TfiI GAWTC 2 cut(s) 618, 1080
Tru1I TTAA 5 cut(s) 23, 215, 401, 593, 1312
Tru9I TTAA 5 cut(s) 23, 215, 401, 593, 1312
TspDTI ATGAA 6 cut(s) 293, 671, 984, 1166, 1241, 1270
TspGWI ACGGA 5 cut(s) 182, 262, 560, 640, 1258
XapI RAATTY 8 cut(s) 263, 281, 641, 659, 767, 833, 1261, 1288
XceI RCATGY 3 cut(s) 369, 747, 1049
XmnI GAANNNNTTC 1 cut(s) 1277
XspI CTAG 2 cut(s) 803, 1004
ZraI GACGTC 1 cut(s) 929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.