Rorug02G0174000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003701
Physical Location & Seq
Reverse (-)
15850372 .. 15940653
90282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0174000.1

Sequence Viewer

Length: 696 bp
ATGTCCTCGCATGCTGGGGGTTTGCCGAAAGAGCAGGAGCACGCAATCATGGTCTCCGCCCTTAAACAGGTCATCGGCGGCGGCGGCATTGCCTCCAGAAGCAGCGGACACTCTCGGCCAGCAGTTGAAGGTGGAAGTGGAACAAAGAAGGTTGTGATATCAATTTCGGATGGCGACACGTGCCAGGTGTGCAGGATCGACGGCTGCCTCGGCTGCGAGTTCTTCCCGCCAAGCAAGCAGGACAAAGGGAAAAGGATCAAGAAGGGCAAGTACAGGGGAGTGAGGCAGAGGCCATGGGGGAAATGGGCGGCGGAGATTCGGGACCCGCGGAGGGCGGTGAGGGTTTGGCTCGGGACGTTCAAGACGGCGGAGGAAGCGGCCAGGGCTTATGACAAGGCGGCGATCGAGTTCCGAGGAGAGAGAGCGAAGCTCAACTTCCCACTAACCAGTGAAGCTGGTACTACTAATACTCCTATTAGTGCAGCATTGGCCAAGCAAAGCATGGAGACTGGTGAGGTCAATCAAGTCGACGCGAGTTCTGTGAAGTCAGCGAAGCACGAAGAGGGACAGAGTAGTGATGTTAAGGACGAAGACATCCATCGCTTCATCTGGGAAATGCTTAAAGATGACGATGAAGATTTAACTTTAAGCATATGGGCAGTGGCGGAACATGAAAGTTATTTTTGGAGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

231

Amino Acids

25.34

Weight (kDa)

8.29

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 90 - 139 1.3e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 528
AccII CGCG 2 cut(s) 328, 533
AclWI GGATC 2 cut(s) 203, 263
AcoI YGGCCR 3 cut(s) 116, 378, 489
AcvI CACGTG 1 cut(s) 180
AfaI GTAC 2 cut(s) 272, 460
AfiI CCNNNNNNNGG 2 cut(s) 67, 331
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 2 cut(s) 128, 361
AjnI CCWGG 2 cut(s) 183, 380
AluBI AGCT 2 cut(s) 430, 455
AluI AGCT 2 cut(s) 430, 455
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 2 cut(s) 58, 500
AlwI GGATC 2 cut(s) 203, 263
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 4 cut(s) 116, 290, 378, 489
ApeKI GCWGC 4 cut(s) 102, 204, 213, 482
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 349, 524
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 491
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BbrPI CACGTG 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 4 cut(s) 114, 191, 200, 494
BccI CCATC 2 cut(s) 164, 606
BceAI ACGGC 2 cut(s) 217, 381
BciT130I CCWGG 2 cut(s) 185, 382
BcoDI GTCTC 2 cut(s) 58, 500
Bme1390I CCNGG 2 cut(s) 185, 382
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 323, 324
BmrFI CCNGG 2 cut(s) 185, 382
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 414, 446
BpmI CTGGAG 1 cut(s) 79
BsaAI YACGTR 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 5 cut(s) 208, 293, 326, 381, 412
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 331
Bse1I ACTGG 2 cut(s) 447, 514
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 2 cut(s) 185, 382
BseDI CCNNGG 5 cut(s) 208, 293, 326, 381, 412
BseGI GGATG 2 cut(s) 175, 594
BseLI CCNNNNNNNGG 2 cut(s) 67, 331
BseMI GCAATG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 447, 514
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 4 cut(s) 114, 191, 200, 494
BseYI CCCAGC 1 cut(s) 14
BsgI GTGCAG 2 cut(s) 211, 501
Bsh1236I CGCG 2 cut(s) 328, 533
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 4 cut(s) 118, 292, 380, 491
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 350
BslFI GGGAC 3 cut(s) 335, 367, 579
BslI CCNNNNNNNGG 2 cut(s) 67, 331
BsmAI GTCTC 2 cut(s) 58, 500
BsmFI GGGAC 3 cut(s) 335, 367, 579
BsnI GGCC 4 cut(s) 118, 292, 380, 491
Bso31I GGTCTC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 350
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 3 cut(s) 195, 255, 402
Bsp19I CCATGG 1 cut(s) 293
BspANI GGCC 4 cut(s) 118, 292, 380, 491
BspFNI CGCG 2 cut(s) 328, 533
BspLI GGNNCC 2 cut(s) 323, 324
BspPI GGATC 2 cut(s) 203, 263
BspTNI GGTCTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 87
BsrI ACTGG 2 cut(s) 447, 514
BssECI CCNNGG 5 cut(s) 208, 293, 326, 381, 412
BssMI GATC 3 cut(s) 195, 255, 402
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 2 cut(s) 185, 382
Bst6I CTCTTC 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 4 cut(s) 12, 42, 120, 236
BstDSI CCRYGG 2 cut(s) 293, 326
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 175, 594
BstFNI CGCG 2 cut(s) 328, 533
BstKTI GATC 3 cut(s) 198, 258, 405
BstMAI GTCTC 2 cut(s) 58, 500
BstMBI GATC 3 cut(s) 195, 255, 402
BstMCI CGRYCG 1 cut(s) 405
BstNI CCWGG 2 cut(s) 185, 382
BstNSI RCATGY 1 cut(s) 14
BstSCI CCNGG 2 cut(s) 183, 380
BstUI CGCG 2 cut(s) 328, 533
BstV1I GCAGC 4 cut(s) 114, 191, 200, 494
BstV2I GAAGAC 1 cut(s) 597
BsuRI GGCC 4 cut(s) 118, 292, 380, 491
BtgI CCRYGG 2 cut(s) 293, 326
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 2 cut(s) 175, 594
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 2 cut(s) 454, 666
Cac8I GCNNGC 4 cut(s) 12, 42, 120, 236
Cfr13I GGNCC 1 cut(s) 322
Cfr42I CCGCGG 1 cut(s) 329
CseI GACGC 1 cut(s) 539
Csp6I GTAC 2 cut(s) 271, 459
CviAII CATG 5 cut(s) 11, 49, 294, 502, 671
CviQI GTAC 2 cut(s) 271, 459
DpnI GATC 3 cut(s) 197, 257, 404
DpnII GATC 3 cut(s) 195, 255, 402
EaeI YGGCCR 3 cut(s) 116, 378, 489
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 4 cut(s) 46, 326, 383, 680
Eco130I CCWWGG 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 58
Eco32I GATATC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 322
Eco72I CACGTG 1 cut(s) 180
Eco88I CYCGRG 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 2 cut(s) 183, 380
EcoRV GATATC 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 5 cut(s) 14, 52, 297, 505, 674
FaiI YATR 8 cut(s) 12, 50, 295, 390, 503, 653, 655, 672
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FaqI GGGAC 3 cut(s) 335, 367, 579
FatI CATG 5 cut(s) 10, 48, 293, 501, 670
FauI CCCGC 2 cut(s) 234, 333
FauNDI CATATG 1 cut(s) 653
FblI GTMKAC 1 cut(s) 528
FokI GGATG 2 cut(s) 182, 581
GsaI CCCAGC 1 cut(s) 18
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 4 cut(s) 118, 292, 380, 491
HgaI GACGC 1 cut(s) 539
Hin1II CATG 5 cut(s) 14, 52, 297, 505, 674
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 1 cut(s) 316
HphI GGTGA 2 cut(s) 349, 524
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 2 cut(s) 169, 413
Hpy188III TCNNGA 5 cut(s) 96, 259, 320, 352, 361
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 2 cut(s) 203, 533
HpyAV CCTTC 3 cut(s) 122, 142, 256
HpyCH4IV ACGT 2 cut(s) 179, 356
HpyCH4V TGCA 2 cut(s) 192, 482
HpySE526I ACGT 2 cut(s) 179, 356
Hsp92II CATG 5 cut(s) 14, 52, 297, 505, 674
KflI GGGWCCC 1 cut(s) 322
KspI CCGCGG 1 cut(s) 329
Kzo9I GATC 3 cut(s) 195, 255, 402
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 4 cut(s) 114, 191, 200, 494
MaeII ACGT 2 cut(s) 179, 356
MalI GATC 3 cut(s) 197, 257, 404
MboI GATC 3 cut(s) 195, 255, 402
MboII GAAGA 4 cut(s) 214, 572, 602, 647
MhlI GDGCHC 1 cut(s) 42
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 1 cut(s) 162
MluNI TGGCCA 1 cut(s) 491
Mox20I TGGCCA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 491
MseI TTAA 5 cut(s) 63, 582, 621, 641, 647
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 2 cut(s) 105, 328
MspR9I CCNGG 2 cut(s) 185, 382
MvaI CCWGG 2 cut(s) 185, 382
MvnI CGCG 2 cut(s) 328, 533
NcoI CCATGG 1 cut(s) 293
NdeI CATATG 1 cut(s) 653
NdeII GATC 3 cut(s) 195, 255, 402
NlaIII CATG 5 cut(s) 14, 52, 297, 505, 674
NlaIV GGNNCC 2 cut(s) 323, 324
NmeAIII GCCGAG 2 cut(s) 94, 189
NspI RCATGY 1 cut(s) 14
PaeI GCATGC 1 cut(s) 14
PfeI GAWTC 1 cut(s) 316
Ple19I CGATCG 1 cut(s) 405
PmaCI CACGTG 1 cut(s) 180
PmlI CACGTG 1 cut(s) 180
Ppu21I YACGTR 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 322
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 2 cut(s) 183, 380
PspCI CACGTG 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 14
PspGI CCWGG 2 cut(s) 183, 380
PspN4I GGNNCC 2 cut(s) 323, 324
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PvuI CGATCG 1 cut(s) 405
RsaI GTAC 2 cut(s) 272, 460
RsaNI GTAC 2 cut(s) 271, 459
SacII CCGCGG 1 cut(s) 329
SalI GTCGAC 1 cut(s) 527
SaqAI TTAA 5 cut(s) 63, 582, 621, 641, 647
Sau3AI GATC 3 cut(s) 195, 255, 402
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 2 cut(s) 185, 382
SduI GDGCHC 1 cut(s) 42
SetI ASST 9 cut(s) 72, 133, 153, 182, 189, 359, 432, 457, 519
Sfr303I CCGCGG 1 cut(s) 329
SgrBI CCGCGG 1 cut(s) 329
SinI GGWCC 1 cut(s) 322
SphI GCATGC 1 cut(s) 14
Sse9I AATT 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 183, 380
StyI CCWWGG 1 cut(s) 293
TaiI ACGT 2 cut(s) 182, 359
TaqI TCGA 3 cut(s) 198, 405, 528
TasI AATT 1 cut(s) 162
TatI WGTACW 1 cut(s) 270
TauI GCSGC 6 cut(s) 81, 84, 87, 311, 380, 401
TfiI GAWTC 1 cut(s) 316
Tru1I TTAA 5 cut(s) 63, 582, 621, 641, 647
Tru9I TTAA 5 cut(s) 63, 582, 621, 641, 647
TscAI CASTG 2 cut(s) 454, 666
TseI GCWGC 4 cut(s) 102, 204, 213, 482
TspDTI ATGAA 3 cut(s) 595, 648, 687
TspRI CASTG 2 cut(s) 454, 666
VpaK11BI GGWCC 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 65
XceI RCATGY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 2 cut(s) 300, 499
XmiI GTMKAC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.