Rroxscaffold_2G00132380

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003701
Physical Location & Seq
Forward (+)
69385519 .. 69392932
7414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132380.1

Sequence Viewer

Length: 747 bp
ATGGAAGAGATAGTATCAACAAAAGAATACAGTGAAGAAAACAAGGATAACATGTTTCCTAAGTTGGAGAAATTGGAGCTAAGTGATCTTCCTCACCTCACTAGATTCTGCTCAGGAGGTTATGTTGAATTTGCATCTCTGGAGAGATTGCAACTAGAGAAGTGTACAAAGTTGGAGACTTTCATCTCTAATCATGTGTGCTGCACCGTAGGGTTTCCAAGGTTGGAGATATTGACCATCAATGGCTTACCTAAGCTGACAACAATGTGGCACACCCAACTTGCTCGAAACTCTTTTTGCAAACTCAGGGACGTTACAGTTAAACGATGCAATAGTCTTAAAACCATTCTTCCAGACTCGATAATAGCTGGAGCTTTTCAGGAGCTACAAAAGTTGACAGTGGAGGACTGTGTAGTGGAGGAAATAGTTGTTAGATTCTATCCAAGTATGCTCGCTTTCGATTGGTCACACCTCAAAACATTAGTGTTTGATTGTCGGCCAGGTGAATATTTTTACACCGGAATTTTTCAGGCCTTATCGAGAAAACACATGACTCGGGGAGTCGGTCTATTCCGCTTAAACAATCTTCGTTCGATCGATAAGGTTGCAACATTTGGAACTTACCGATTTAGTAAGTTTGGAACGCTTTTGCCCAAGAAATTGCAGGGTGAAGGTCCCGAAACCTTGGCAACTTTAGAGGTAAAATGGTGCTCTTTCGAGTCAAGATTTCCTCCGATGGGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

248

Amino Acids

28.55

Weight (kDa)

8.32

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 73 - 137 5.4e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 574
AcoI YGGCCR 1 cut(s) 497
AcsI RAATTY 2 cut(s) 128, 522
AfaI GTAC 2 cut(s) 166, 743
AfiI CCNNNNNNNGG 1 cut(s) 737
AflIII ACRYGT 1 cut(s) 51
AgsI TTSAA 1 cut(s) 128
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 5 cut(s) 79, 256, 368, 374, 385
AluI AGCT 5 cut(s) 79, 256, 368, 374, 385
Alw21I GWGCWC 1 cut(s) 713
Alw26I GTCTC 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 2 cut(s) 497, 531
ApeKI GCWGC 1 cut(s) 201
ApoI RAATTY 2 cut(s) 128, 522
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 86, 515, 680
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 713
BbvI GCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 245, 730
BcgI CGANNNNNNTGC 2 cut(s) 587, 621
BciT130I CCWGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 3 cut(s) 102, 155, 745
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 674
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 1 cut(s) 501
BmsI GCATC 2 cut(s) 143, 317
BpmI CTGGAG 2 cut(s) 161, 390
Bpu10I CCTNAGC 2 cut(s) 112, 252
Bsa29I ATCGAT 1 cut(s) 597
BsaJI CCNNGG 2 cut(s) 218, 684
BsaWI WCCGGW 1 cut(s) 518
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 737
BseBI CCWGG 1 cut(s) 501
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 218, 684
BseLI CCNNNNNNNGG 1 cut(s) 737
BseMII CTCAG 2 cut(s) 126, 319
BseXI GCAGC 1 cut(s) 188
BsgI GTGCAG 1 cut(s) 187
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 2 cut(s) 499, 533
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 713
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 1 cut(s) 519
BslFI GGGAC 2 cut(s) 323, 660
BslI CCNNNNNNNGG 1 cut(s) 737
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 2 cut(s) 323, 660
BsnI GGCC 2 cut(s) 499, 533
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 713
Bsp1407I TGTACA 1 cut(s) 164
Bsp143I GATC 2 cut(s) 85, 594
BspACI CCGC 1 cut(s) 574
BspANI GGCC 2 cut(s) 499, 533
BspCNI CTCAG 2 cut(s) 125, 318
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 676
BsrGI TGTACA 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 218, 684
BssMI GATC 2 cut(s) 85, 594
BssT1I CCWWGG 2 cut(s) 218, 684
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 5 cut(s) 32, 208, 319, 400, 410
BstAUI TGTACA 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 5 cut(s) 60, 80, 112, 252, 305
BstKTI GATC 2 cut(s) 88, 597
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 2 cut(s) 85, 594
BstMCI CGRYCG 1 cut(s) 597
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 499
BstV1I GCAGC 1 cut(s) 188
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 2 cut(s) 499, 533
BsuTUI ATCGAT 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 37, 405
Cac8I GCNNGC 1 cut(s) 453
Cfr13I GGNCC 1 cut(s) 674
ClaI ATCGAT 1 cut(s) 597
Csp6I GTAC 2 cut(s) 165, 742
CviAII CATG 3 cut(s) 52, 194, 550
CviJI RGCY 8 cut(s) 79, 246, 256, 368, 374, 385, 499, 533
CviKI_1 RGCY 8 cut(s) 79, 246, 256, 368, 374, 385, 499, 533
CviQI GTAC 2 cut(s) 165, 742
DdeI CTNAG 5 cut(s) 60, 80, 112, 252, 305
DpnI GATC 2 cut(s) 87, 596
DpnII GATC 2 cut(s) 85, 594
EaeI YGGCCR 1 cut(s) 497
Eco130I CCWWGG 2 cut(s) 218, 684
Eco147I AGGCCT 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 2 cut(s) 218, 684
ErhI CCWWGG 2 cut(s) 218, 684
FaeI CATG 3 cut(s) 55, 197, 553
FaiI YATR 5 cut(s) 53, 123, 195, 449, 551
FaqI GGGAC 2 cut(s) 323, 660
FatI CATG 3 cut(s) 51, 193, 549
Fnu4HI GCNGC 1 cut(s) 202
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 3 cut(s) 102, 155, 745
GluI GCNGC 1 cut(s) 202
GsuI CTGGAG 2 cut(s) 161, 390
HaeIII GGCC 2 cut(s) 499, 533
HapII CCGG 1 cut(s) 519
Hin1II CATG 3 cut(s) 55, 197, 553
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HinfI GANTC 6 cut(s) 105, 356, 435, 553, 561, 719
HpaII CCGG 1 cut(s) 519
HphI GGTGA 3 cut(s) 86, 515, 680
Hpy166II GTNNAC 2 cut(s) 165, 396
Hpy188I TCNGA 1 cut(s) 735
Hpy188III TCNNGA 7 cut(s) 114, 140, 353, 380, 540, 677, 723
Hpy8I GTNNAC 2 cut(s) 165, 396
HpyAV CCTTC 1 cut(s) 665
HpyCH4III ACNGT 5 cut(s) 32, 208, 319, 400, 410
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 134, 151, 204, 300, 330, 608, 664
HpyF3I CTNAG 5 cut(s) 60, 80, 112, 252, 305
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 55, 197, 553
Kzo9I GATC 2 cut(s) 85, 594
LmnI GCTCC 3 cut(s) 76, 371, 382
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 2 cut(s) 143, 317
MaeI CTAG 3 cut(s) 102, 155, 745
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 2 cut(s) 313, 465
MalI GATC 2 cut(s) 87, 596
MboI GATC 2 cut(s) 85, 594
MboII GAAGA 5 cut(s) 17, 47, 80, 341, 578
MhlI GDGCHC 1 cut(s) 713
MluCI AATT 4 cut(s) 71, 128, 522, 659
MlyI GAGTC 4 cut(s) 350, 547, 570, 728
MmeI TCCRAC 3 cut(s) 45, 153, 204
MnlI CCTC 8 cut(s) 102, 107, 110, 397, 412, 482, 691, 741
MseI TTAA 3 cut(s) 321, 339, 578
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
NdeII GATC 2 cut(s) 85, 594
NlaIII CATG 3 cut(s) 55, 197, 553
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 1 cut(s) 465
NspI RCATGY 1 cut(s) 55
PceI AGGCCT 1 cut(s) 533
PciI ACATGT 1 cut(s) 51
PfeI GAWTC 2 cut(s) 105, 435
PkrI GCNGC 1 cut(s) 203
Ple19I CGATCG 1 cut(s) 597
PleI GAGTC 4 cut(s) 350, 547, 569, 727
PpsI GAGTC 4 cut(s) 350, 547, 569, 727
PpuMI RGGWCCY 1 cut(s) 674
PscI ACATGT 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 676
PspPI GGNCC 1 cut(s) 674
PspPPI RGGWCCY 1 cut(s) 674
PvuI CGATCG 1 cut(s) 597
RsaI GTAC 2 cut(s) 166, 743
RsaNI GTAC 2 cut(s) 165, 742
SaqAI TTAA 3 cut(s) 321, 339, 578
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 2 cut(s) 85, 594
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 4 cut(s) 350, 547, 570, 728
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 1 cut(s) 713
SfaNI GCATC 2 cut(s) 143, 317
SinI GGWCC 1 cut(s) 674
Sse9I AATT 4 cut(s) 71, 128, 522, 659
SseBI AGGCCT 1 cut(s) 533
SsiI CCGC 1 cut(s) 574
SspI AATATT 1 cut(s) 509
SspMI CTAG 3 cut(s) 102, 155, 745
StuI AGGCCT 1 cut(s) 533
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 2 cut(s) 218, 684
TaaI ACNGT 5 cut(s) 32, 208, 319, 400, 410
TaiI ACGT 1 cut(s) 315
TaqI TCGA 7 cut(s) 286, 359, 459, 539, 593, 597, 717
TaqII GACCGA 1 cut(s) 554
TasI AATT 4 cut(s) 71, 128, 522, 659
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 2 cut(s) 105, 435
Tru1I TTAA 3 cut(s) 321, 339, 578
Tru9I TTAA 3 cut(s) 321, 339, 578
TscAI CASTG 2 cut(s) 37, 405
TseFI GTSAC 1 cut(s) 465
TseI GCWGC 1 cut(s) 201
Tsp45I GTSAC 1 cut(s) 465
TspDTI ATGAA 1 cut(s) 172
TspRI CASTG 2 cut(s) 37, 405
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 2 cut(s) 128, 522
XceI RCATGY 1 cut(s) 55
XspI CTAG 3 cut(s) 102, 155, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.