Rh7AG198500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0003701
Physical Location & Seq
Reverse (-)
17908668 .. 17924560
15893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG198500.1

Sequence Viewer

Length: 3837 bp
ATGGCGATTAGATTTACGGAGGCAATTCTTTTTAAAACTAAGGAGACAATTGAACTCGTAGGACGCCAAGTGGGATATGTAATTCACTGCAAAAGCAACCTTCAAAGACTGCAGGCTCAATTGGAAGAATTGAACGCCGCCCGGGAGGCTGTAGAGGATAAAGTTGTGGAAGCTGAAGACAAAGGTGAAGCAGTTCAACCAGATGTCCAGTTGTGGCTACAGGAAGTGGGCAACATCAATGCGCAGGCAGAGGAATTGTTGAAAGATGAAAACCAAGCCAAGCTGAAATGTCTCCTTGGGTTTTGTCCTAATCTGACAATCCGTCATCACCTAGGCAGGAAATCAAAAATATTGGTGCACCATCTTGTTGAACTCTATGAAAGACGAGAATTTCCCGTTATTTCTGTTATTCGGCCGCAAGAGCTTTGGGTTGAGTCCACCGGAGAGTACCTGGCCTTTCCATCAAGGACGTCAATTGTGGCCCAAATCATGGAAGAACTTGAGAATCCTAATACTCATAGGATCGGGGTGTATGGAATTGAAGGTGTGGGTAAAACCACACTAGCAAAAGAAGTCTACAGGCAAGCTACAGAAGATAAAAAGTTATTTGACAATGTGGTTATCCTACTAGATGTGAAAGAAAATCCACACTTGGAAGGAATTCAAAACAAAATTTTAAATAAGTTAGATATGAAGATTTTTGATGATGAGAATTTAGATGGAAGAGCAAGTCGTACTTCTGCCGATATACAGGACAAAAACATCCTTGTAATTCTGGATGATGTTTGGGAACAAATTGACTTGGAGGCTCTCCAACTTCCAAGTGTGGCTACTTGTAAGATATTGCTGACATGTACAAGTAGAGAATCGTTATCTGGTATGAACACGCAGGTGGATTTTCAGCTTGACATCTTGGTGGAAGAAGAGGCTTGGATTCTATTTAAGAAGAAGGCAGGCGACGTTGTTAAGAATTATGCTATACGAGAAGTAGCACTCCAAGTAGCCGAATGTTGTAGTGGCTTGCCTATTTTAGTTGTCACAGTTGCAAGCGCTTTGAAGAAACAAACTACTTCACCTCCATGGAGAGATGCATTGAGATGCCTAAAAAGCCAAGAATTTACAGAGAGCCCATACTTGTGTCTGGGGTGGAGTTACGATCAGCTGAAGGATAAGGAACTTGAGCAGTTGTTCTTGCTATGTGGATTTGCTATATGGGGAAAGGATATTTATTTGCCAGCCTTGCTGAAATATAGTATGGGTTTGGGTCTCTTTAGAAAGGGATACACAGTGGAGGAAGCACAAAATACATTGCTTAAATGGGTTGGAAAATTAAAAGATTCTTGTCTACTGACAGACAGTGATGATAGCAGATATGTCAAAATGCATAACCTTGTGCGTGATGTTGCTAACCGGATTGCACTCAGAGATGAACACATCTTATCATCAGTCGATGATAGGGGAAATTATGAACTTAAGGAATGGCTAGATAAGGATTTCTTCAAAAAGTGCACTATGAAATCTTTTCAATCTAGTACTGTTCCTGTGCTTTCTGAAGTACCTAGGATATACCCCAAATTAAAGCTGTTCTATTTACACGGTACAGGTGATGTGTCAGTGGAAATCCCAACAAACTTTTTTAGAGAGATTGAGGAACTAAAAGTGTTGGATTTAACAAGACTGCATATGCTGTCACTACCTTCCTCTCTTCAGTACTTGAAGAATCTGCATGCCCTATTTTTAGATCAATGCATAGTGGGAGACACTGCTTTAGTTGGGGAGCTAAGTAACTTGGAAATTCTTAGCTTTTTCCAGTCCAATGTCAAACAATTACCAAAGGAAATAGGGAAACTGACCCGTCTTCAGTTGCTGGAATTATCTGATTGCTCTGAACTCGAAGTTATTTCACCTGGTGTCATCTCAAGCTTGGAGAGACTACAAGAATTGAGAATGAGAAACAGCTTCAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTAGTGAGTTGAAGCATTTGTCTCAGCTATCTGTATTAGAGGTACATATTCCGAATACTGACATCCTTCCAGCAAACTTGTTCTCCCACGAGTTCGAAAGATTCAATATTGTTATTGGAGATGCATGGGTTGACAGATATACATCACGAGATAAATTTATGGCTGAGACGACCCTCAAGACACTAAAACTCAAACTCACTACCAGCATTAAAGAATTGGATGAAGGATTAATATGGCTGCTGGAGAGATGTGAAGACTTGTCCTTAAGTGAGACACAGAATGCTAATAATATTGACCATTATTTAGATACTGAAGATTCTGAGCATCCGCAACATCTGCAAGTCATAAACATCTATAAGATTGGTTTGCCCAACTTAACAAGATTGGCTGTTCACCAATGTGATGGTTTAAGTTTCTTATTGTCATCTTCCGTGGCAAAAAGTTTTGTACAACTTAAACATCTTCGGATATCTGAATGTCAAATCATGAAAGAGGTAGTATTGTCAGAAGGTCACGGTGAAGAAAAGGATAACTTGTTTCCGAAGTTAGAACAATTGGAGCTAAATGATCTTCCTTGTCTCACTAGATTCTGTTCAGGAAGTTATGTTGAGTTTACAGCTCTGAAGAGGTCGCAATTGGAGAAGTGTCCTAAATTGGAGACATTCATCTTTAATCATTTGAGTAACAGAATTCAAATCTACAAAGAAATTAAAGAAAGGGGCACAAATGATGAGAATGTTGAGACAGTTGCACCATACTTTCTTTTAGATGAAAAGGTGAAAACGTTGGGGTTGCATGGAATGGAGATCAAGCTCATGCATCTCTCAGAAGAGAGCCCAGTTTTTCCACTGTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAACCTAAAGTTGTCTGCAATATCCTTTCGGAATCTAACAATTATAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGACAAGTTATTCGGTAGCTCAAAGTTTAGTGGGACTGAAAAAATTGAAAGTTGATGAATGTAAAAATATGAAAGAAATCTTCACAAGTGAAGGAATTGAAGAAGATGCAAGTAATTGTGAGATTGTTTTCAGCCGGTTGCAACATTTGGAACTCAGTGATTTAATAAGTTTGGAAAGGTTTTGCTCGAGAAATTGCATTGTGAAAGTCCCATCCTTGCAAAGCAGTGCTTTACAGGTAAATTGGTGCCCATTCCAGTTGAAGATTTCCACTGAAAGGGTACTAGAAGAAGTTTCCGATATGCAACAGGATGTACATAAGTCGCATACTGGTAAAGAAGAGGTTCTCGACACAACTGAGTCTGCTTGTAGCCTGGAAACAAATCAGAAACAGCACATCCCAAACACCTCCGAAGTTTCTAAAGAAATCGAGTTTAATGCAGGAGAACTCCATGATGACAGACTTGTAAACAACGACGGGCCTCTATTGCCTGGCAATATTGCTGAAAGTAGTGAACATGGAATCAAAGCTGTCATTAAAGAAACTCCTATCAATTTCTTGGAGCATGCTGAGGTTATGGACCTACATCGGAAGCAAATTGTCACCAATACGCCAAAGAATCTTCAGGAGGCTGATTTTGGCATGGAAGAGAGCCTCATTGAACCTTTGAATGCAAATGAAGGCAGACCACTTCAATTGAAACAACTTAATGGGGTGCAAACTGATTCGTTGCTGCAAAGCATATCTGAGCTTGATGCCGACATAAAACATTTGCAGGAAGAATTAAGGGCCAAAGAAGCAGAGAGGATAGATAAACAGCTAAGGCTTGCCGACAGACTTGCGACAGTTGATCGGTTGTCCGATTCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1278

Amino Acids

145.73

Weight (kDa)

5.19

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 159 - 319 3.6e-24 NB-ARC domain
LRR_14 PF23598 591 - 686 2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 791 - 888 4.9e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 963 - 1071 4.4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 880
AatII GACGTC 1 cut(s) 473
Acc16I TGCGCA 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 3210
AccB7I CCANNNNNTGG 1 cut(s) 490
AccI GTMKAC 2 cut(s) 576, 1345
AciI CCGC 3 cut(s) 138, 416, 2330
AclI AACGTT 1 cut(s) 2786
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 9 cut(s) 389, 660, 672, 712, 1115, 1794, 2156, 2691, 2859
AcuI CTGAAG 8 cut(s) 195, 1184, 1572, 1691, 1844, 2334, 2645, 3572
AcyI GRCGYC 2 cut(s) 64, 470
AdeI CACNNNGTG 1 cut(s) 1910
AfeI AGCGCT 1 cut(s) 1051
AfiI CCNNNNNNNGG 2 cut(s) 490, 3240
AflII CTTAAG 2 cut(s) 1472, 2266
AflIII ACRYGT 1 cut(s) 851
AhdI GACNNNNNGTC 1 cut(s) 321
AjnI CCWGG 4 cut(s) 450, 1906, 3336, 3454
AleI CACNNNNGTG 2 cut(s) 890, 2400
Alw21I GWGCWC 2 cut(s) 360, 1511
Alw44I GTGCAC 2 cut(s) 356, 1507
AlwI GGATC 1 cut(s) 530
AlwNI CAGNNNCTG 1 cut(s) 1867
Ama87I CYCGRG 2 cut(s) 141, 3151
Aor51HI AGCGCT 1 cut(s) 1051
AoxI GGCC 5 cut(s) 413, 453, 480, 3443, 3753
ApaLI GTGCAC 2 cut(s) 356, 1507
ApeKI GCWGC 2 cut(s) 2239, 3697
ApoI RAATTY 9 cut(s) 389, 660, 672, 712, 1115, 1794, 2156, 2691, 2859
ArsI GACNNNNNNTTYG 2 cut(s) 2959, 2991
AseI ATTAAT 1 cut(s) 2231
Asp700I GAANNNNTTC 3 cut(s) 192, 660, 3306
AspA2I CCTAGG 2 cut(s) 331, 1559
AspLEI GCGC 2 cut(s) 244, 1052
AspS9I GGNCC 4 cut(s) 481, 3443, 3544, 3753
AsuC2I CCSGG 2 cut(s) 142, 143
AsuII TTCGAA 1 cut(s) 2097
AvaI CYCGRG 2 cut(s) 141, 3151
AvaII GGWCC 1 cut(s) 3544
AvrII CCTAGG 2 cut(s) 331, 1559
BaeGI GKGCMC 4 cut(s) 360, 1511, 2726, 3215
BanI GGYRCC 1 cut(s) 3210
BanII GRGCYC 2 cut(s) 1130, 2840
BauI CACGAG 2 cut(s) 2090, 2148
BbsI GAAGAC 3 cut(s) 183, 1850, 2262
Bbv12I GWGCWC 2 cut(s) 360, 1511
BbvCI CCTCAGC 1 cut(s) 3534
BbvI GCAGC 2 cut(s) 2226, 3684
BccI CCATC 5 cut(s) 369, 469, 713, 2399, 3184
BcgI CGANNNNNNTGC 4 cut(s) 3383, 3417, 3785, 3819
BciT130I CCWGG 4 cut(s) 452, 1908, 3338, 3456
BciVI GTATCC 1 cut(s) 1274
BcnI CCSGG 2 cut(s) 142, 143
BfaI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2586, 2864, 3248
BfmI CTRYAG 5 cut(s) 110, 150, 218, 577, 588
BfoI RGCGCY 1 cut(s) 1053
BfrI CTTAAG 2 cut(s) 1472, 2266
BfuAI ACCTGC 1 cut(s) 880
BfuI GTATCC 1 cut(s) 1274
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 4 cut(s) 138, 416, 2240, 3698
BlnI CCTAGG 2 cut(s) 331, 1559
BlsI GCNGC 4 cut(s) 139, 417, 2241, 3699
BmcAI AGTACT 2 cut(s) 1534, 1712
Bme1390I CCNGG 6 cut(s) 142, 143, 452, 1908, 3338, 3456
Bme18I GGWCC 1 cut(s) 3544
BmeRI GACNNNNNGTC 1 cut(s) 321
BmeT110I CYCGRG 2 cut(s) 141, 3151
BmgT120I GGNCC 4 cut(s) 481, 3443, 3544, 3753
BmiI GGNNCC 1 cut(s) 3212
BmrFI CCNGG 6 cut(s) 142, 143, 452, 1908, 3338, 3456
BmrI ACTGGG 1 cut(s) 2834
BmsI GCATC 7 cut(s) 1078, 1088, 2113, 2335, 2830, 3061, 3709
BmuI ACTGGG 1 cut(s) 2834
BpiI GAAGAC 3 cut(s) 183, 1850, 2262
BpmI CTGGAG 1 cut(s) 2264
Bpu10I CCTNAGC 2 cut(s) 3534, 3785
Bpu14I TTCGAA 1 cut(s) 2097
BpuEI CTTGAG 4 cut(s) 521, 1199, 1903, 2162
BpuMI CCSGG 2 cut(s) 142, 143
BsaBI GATNNNNATC 1 cut(s) 2143
BsaHI GRCGYC 2 cut(s) 64, 470
BsaI GGTCTC 1 cut(s) 1271
BsaJI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2433
BsaWI WCCGGW 2 cut(s) 440, 1410
BsaXI ACNNNNNCTCC 4 cut(s) 1060, 1090, 2069, 2099
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 3240
Bse118I RCCGGY 1 cut(s) 3099
Bse1I ACTGG 5 cut(s) 208, 1810, 2840, 3220, 3298
Bse3DI GCAATG 1 cut(s) 1307
Bse8I GATNNNNATC 1 cut(s) 2143
BseBI CCWGG 4 cut(s) 452, 1908, 3338, 3456
BseDI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2433
BseGI GGATG 8 cut(s) 762, 784, 2064, 2227, 2326, 3176, 3280, 3360
BseJI GATNNNNATC 1 cut(s) 2143
BseLI CCNNNNNNNGG 2 cut(s) 490, 3240
BseMI GCAATG 1 cut(s) 1307
BseMII CTCAG 9 cut(s) 1435, 2039, 2157, 2313, 2841, 3133, 3312, 3525, 3702
BseNI ACTGG 5 cut(s) 208, 1810, 2840, 3220, 3298
BseSI GKGCMC 4 cut(s) 360, 1511, 2726, 3215
BseX3I CGGCCG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 2226, 3684
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 5 cut(s) 415, 455, 482, 3445, 3755
BshNI GGYRCC 1 cut(s) 3210
BsiEI CGRYCG 1 cut(s) 416
BsiHKAI GWGCWC 2 cut(s) 360, 1511
BsiHKCI CYCGRG 2 cut(s) 141, 3151
BsiSI CCGG 4 cut(s) 142, 441, 1411, 3100
BslFI GGGAC 2 cut(s) 3012, 3158
BslI CCNNNNNNNGG 2 cut(s) 490, 3240
BsmBI CGTCTC 1 cut(s) 2162
BsmFI GGGAC 2 cut(s) 3012, 3158
BsmI GAATGC 2 cut(s) 2287, 3640
BsnI GGCC 5 cut(s) 415, 455, 482, 3445, 3755
Bso31I GGTCTC 1 cut(s) 1271
BsoBI CYCGRG 2 cut(s) 141, 3151
Bsp119I TTCGAA 1 cut(s) 2097
Bsp1286I GDGCHC 6 cut(s) 360, 1130, 1511, 2726, 2840, 3215
Bsp1407I TGTACA 3 cut(s) 854, 2449, 3277
Bsp143I GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
Bsp19I CCATGG 1 cut(s) 1079
BspACI CCGC 3 cut(s) 138, 416, 2330
BspANI GGCC 5 cut(s) 415, 455, 482, 3445, 3755
BspCNI CTCAG 9 cut(s) 1434, 2038, 2158, 2314, 2840, 3132, 3313, 3526, 3703
BspHI TCATGA 1 cut(s) 2487
BspLI GGNNCC 1 cut(s) 3212
BspMAI CTGCAG 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 718
BspT104I TTCGAA 1 cut(s) 2097
BspT107I GGYRCC 1 cut(s) 3210
BspTI CTTAAG 2 cut(s) 1472, 2266
BspTNI GGTCTC 1 cut(s) 1271
BsrDI GCAATG 1 cut(s) 1307
BsrFI RCCGGY 1 cut(s) 3099
BsrGI TGTACA 3 cut(s) 854, 2449, 3277
BsrI ACTGG 5 cut(s) 208, 1810, 2840, 3220, 3298
BssAI RCCGGY 1 cut(s) 3099
BssECI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2433
BssMI GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
BssNI GRCGYC 2 cut(s) 64, 470
BssSI CACGAG 2 cut(s) 2090, 2148
BssT1I CCWWGG 4 cut(s) 295, 331, 1079, 1559
Bst2BI CACGAG 2 cut(s) 2090, 2148
Bst2UI CCWGG 4 cut(s) 452, 1908, 3338, 3456
Bst4CI ACNGT 9 cut(s) 1042, 1288, 1358, 1537, 1598, 2519, 2749, 2853, 3811
Bst6I CTCTTC 7 cut(s) 718, 918, 1710, 2621, 2826, 3297, 3606
BstACI GRCGYC 2 cut(s) 64, 470
BstAFI CTTAAG 2 cut(s) 1472, 2266
BstAPI GCANNNNNTGC 1 cut(s) 2338
BstAUI TGTACA 3 cut(s) 854, 2449, 3277
BstBI TTCGAA 1 cut(s) 2097
BstDSI CCRYGG 2 cut(s) 1079, 2433
BstF5I GGATG 8 cut(s) 762, 784, 2064, 2227, 2326, 3176, 3280, 3360
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 2 cut(s) 244, 1052
BstKTI GATC 6 cut(s) 525, 1159, 1744, 2572, 2811, 3817
BstMBI GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 7 cut(s) 146, 421, 1107, 1240, 2338, 3451, 3761
BstNI CCWGG 4 cut(s) 452, 1908, 3338, 3456
BstNSI RCATGY 3 cut(s) 855, 1730, 3533
BstSCI CCNGG 6 cut(s) 140, 141, 450, 1906, 3336, 3454
BstSFI CTRYAG 5 cut(s) 110, 150, 218, 577, 588
BstSLI GKGCMC 4 cut(s) 360, 1511, 2726, 3215
BstV1I GCAGC 2 cut(s) 2226, 3684
BstV2I GAAGAC 3 cut(s) 183, 1850, 2262
BstXI CCANNNNNNTGG 1 cut(s) 2405
BstZI CGGCCG 1 cut(s) 413
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 5 cut(s) 415, 455, 482, 3445, 3755
BtgI CCRYGG 2 cut(s) 1079, 2433
BtsCI GGATG 8 cut(s) 762, 784, 2064, 2227, 2326, 3176, 3280, 3360
BtsI GCAGTG 3 cut(s) 85, 1761, 3196
BtsIMutI CAGTG 9 cut(s) 85, 1293, 1363, 1620, 1761, 2849, 3127, 3196, 3234
BveI ACCTGC 1 cut(s) 880
CaiI CAGNNNCTG 1 cut(s) 1867
CciI TCATGA 1 cut(s) 2487
CfoI GCGC 2 cut(s) 244, 1052
Cfr10I RCCGGY 1 cut(s) 3099
Cfr13I GGNCC 4 cut(s) 481, 3443, 3544, 3753
Cfr9I CCCGGG 1 cut(s) 141
CseI GACGC 1 cut(s) 72
CsiI ACCWGGT 1 cut(s) 1906
CspCI CAANNNNNGTGG 2 cut(s) 3642, 3677
DpnI GATC 6 cut(s) 524, 1158, 1743, 2571, 2810, 3816
DpnII GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
DraI TTTAAA 2 cut(s) 34, 678
DraIII CACNNNGTG 1 cut(s) 1910
DriI GACNNNNNGTC 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 413
EagI CGGCCG 1 cut(s) 413
Eam1104I CTCTTC 7 cut(s) 718, 918, 1710, 2621, 2826, 3297, 3606
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 7 cut(s) 718, 918, 1710, 2621, 2826, 3297, 3606
EclXI CGGCCG 1 cut(s) 413
Eco130I CCWWGG 4 cut(s) 295, 331, 1079, 1559
Eco24I GRGCYC 2 cut(s) 1130, 2840
Eco31I GGTCTC 1 cut(s) 1271
Eco32I GATATC 1 cut(s) 2472
Eco47I GGWCC 1 cut(s) 3544
Eco47III AGCGCT 1 cut(s) 1051
Eco52I CGGCCG 1 cut(s) 413
Eco57I CTGAAG 8 cut(s) 195, 1184, 1572, 1691, 1844, 2334, 2645, 3572
Eco88I CYCGRG 2 cut(s) 141, 3151
EcoRI GAATTC 2 cut(s) 660, 2691
EcoRII CCWGG 4 cut(s) 450, 1906, 3336, 3454
EcoRV GATATC 1 cut(s) 2472
EcoT14I CCWWGG 4 cut(s) 295, 331, 1079, 1559
EcoT22I ATGCAT 5 cut(s) 1093, 1386, 1751, 2128, 2823
EcoT38I GRGCYC 2 cut(s) 1130, 2840
ErhI CCWWGG 4 cut(s) 295, 331, 1079, 1559
Esp3I CGTCTC 1 cut(s) 2162
FalI AAGNNNNNCTT 8 cut(s) 721, 753, 1481, 1513, 2519, 2551, 3178, 3210
FaqI GGGAC 2 cut(s) 3012, 3158
FauNDI CATATG 1 cut(s) 1683
FblI GTMKAC 2 cut(s) 576, 1345
Fnu4HI GCNGC 4 cut(s) 138, 416, 2240, 3698
FokI GGATG 8 cut(s) 749, 791, 2051, 2234, 2313, 3163, 3287, 3347
FriOI GRGCYC 2 cut(s) 1130, 2840
Fsp4HI GCNGC 4 cut(s) 138, 416, 2240, 3698
FspBI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2586, 2864, 3248
FspI TGCGCA 1 cut(s) 243
GlaI GCGC 2 cut(s) 243, 1051
GluI GCNGC 4 cut(s) 138, 416, 2240, 3698
GsuI CTGGAG 1 cut(s) 2264
HaeII RGCGCY 1 cut(s) 1053
HaeIII GGCC 5 cut(s) 415, 455, 482, 3445, 3755
HapII CCGG 4 cut(s) 142, 441, 1411, 3100
HgaI GACGC 1 cut(s) 72
HhaI GCGC 2 cut(s) 244, 1052
Hin1I GRCGYC 2 cut(s) 64, 470
Hin6I GCGC 2 cut(s) 242, 1050
HinP1I GCGC 2 cut(s) 242, 1050
HincII GTYRAC 1 cut(s) 2134
HindII GTYRAC 1 cut(s) 2134
HindIII AAGCTT 1 cut(s) 1921
HpaII CCGG 4 cut(s) 142, 441, 1411, 3100
Hpy188III TCNNGA 9 cut(s) 776, 2148, 2179, 2488, 2598, 2864, 3153, 3311, 3590
Hpy99I CGWCG 2 cut(s) 962, 3443
HpyCH4III ACNGT 9 cut(s) 1042, 1288, 1358, 1537, 1598, 2519, 2749, 2853, 3811
HpyCH4IV ACGT 3 cut(s) 470, 960, 2786
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 421, 1107, 1240, 2338, 3451, 3761
HpySE526I ACGT 3 cut(s) 470, 960, 2786
Hsp92I GRCGYC 2 cut(s) 64, 470
HspAI GCGC 2 cut(s) 242, 1050
Kzo9I GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 3 cut(s) 1777, 2560, 3526
Lsp1109I GCAGC 2 cut(s) 2226, 3684
LweI GCATC 7 cut(s) 1078, 1088, 2113, 2335, 2830, 3061, 3709
MabI ACCWGGT 1 cut(s) 1906
MaeI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2586, 2864, 3248
MaeII ACGT 3 cut(s) 470, 960, 2786
MaeIII GTNAC 8 cut(s) 1036, 1151, 1689, 1784, 2513, 2684, 2938, 3565
MalI GATC 6 cut(s) 524, 1158, 1743, 2571, 2810, 3816
MboI GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
MfeI CAATTG 6 cut(s) 48, 119, 474, 2555, 2636, 3659
MhlI GDGCHC 6 cut(s) 360, 1130, 1511, 2726, 2840, 3215
MlyI GAGTC 2 cut(s) 443, 3332
MmeI TCCRAC 4 cut(s) 838, 1303, 1644, 2835
Mph1103I ATGCAT 5 cut(s) 1093, 1386, 1751, 2128, 2823
MroXI GAANNNNTTC 3 cut(s) 192, 660, 3306
MslI CAYNNNNRTG 6 cut(s) 890, 914, 1078, 1096, 1135, 2400
MspA1I CMGCKG 1 cut(s) 1162
MspCI CTTAAG 2 cut(s) 1472, 2266
MspI CCGG 4 cut(s) 142, 441, 1411, 3100
MspR9I CCNGG 6 cut(s) 142, 143, 452, 1908, 3338, 3456
MunI CAATTG 6 cut(s) 48, 119, 474, 2555, 2636, 3659
Mva1269I GAATGC 2 cut(s) 2287, 3640
MvaI CCWGG 4 cut(s) 452, 1908, 3338, 3456
MwoI GCNNNNNNNGC 7 cut(s) 146, 421, 1107, 1240, 2338, 3451, 3761
NciI CCSGG 2 cut(s) 142, 143
NcoI CCATGG 1 cut(s) 1079
NdeI CATATG 1 cut(s) 1683
NdeII GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
NlaIV GGNNCC 1 cut(s) 3212
NmuCI GTSAC 4 cut(s) 1036, 1689, 2513, 3565
NsbI TGCGCA 1 cut(s) 243
NsiI ATGCAT 5 cut(s) 1093, 1386, 1751, 2128, 2823
NspI RCATGY 3 cut(s) 855, 1730, 3533
NspV TTCGAA 1 cut(s) 2097
OliI CACNNNNGTG 2 cut(s) 890, 2400
PaeI GCATGC 2 cut(s) 1730, 3533
PaeR7I CTCGAG 1 cut(s) 3151
PagI TCATGA 1 cut(s) 2487
PaqCI CACCTGC 1 cut(s) 880
PciI ACATGT 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 718
PctI GAATGC 2 cut(s) 2287, 3640
PdmI GAANNNNTTC 3 cut(s) 192, 660, 3306
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 4 cut(s) 139, 417, 2241, 3699
PleI GAGTC 2 cut(s) 442, 3331
PpsI GAGTC 2 cut(s) 442, 3331
PscI ACATGT 1 cut(s) 851
PshBI ATTAAT 1 cut(s) 2231
Psp1406I AACGTT 1 cut(s) 2786
Psp6I CCWGG 4 cut(s) 450, 1906, 3336, 3454
PspGI CCWGG 4 cut(s) 450, 1906, 3336, 3454
PspN4I GGNNCC 1 cut(s) 3212
PspPI GGNCC 4 cut(s) 481, 3443, 3544, 3753
PstI CTGCAG 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 1867
PvuII CAGCTG 1 cut(s) 1162
RseI CAYNNNNRTG 6 cut(s) 890, 914, 1078, 1096, 1135, 2400
SapI GCTCTTC 1 cut(s) 718
SatI GCNGC 4 cut(s) 138, 416, 2240, 3698
Sau3AI GATC 6 cut(s) 522, 1156, 1741, 2569, 2808, 3814
Sau96I GGNCC 4 cut(s) 481, 3443, 3544, 3753
ScaI AGTACT 2 cut(s) 1534, 1712
SchI GAGTC 2 cut(s) 443, 3332
ScrFI CCNGG 6 cut(s) 142, 143, 452, 1908, 3338, 3456
SduI GDGCHC 6 cut(s) 360, 1130, 1511, 2726, 2840, 3215
SexAI ACCWGGT 1 cut(s) 1906
SfaNI GCATC 7 cut(s) 1078, 1088, 2113, 2335, 2830, 3061, 3709
SfcI CTRYAG 5 cut(s) 110, 150, 218, 577, 588
Sfr274I CTCGAG 1 cut(s) 3151
SfuI TTCGAA 1 cut(s) 2097
SinI GGWCC 1 cut(s) 3544
SlaI CTCGAG 1 cut(s) 3151
SmaI CCCGGG 1 cut(s) 143
SmiMI CAYNNNNRTG 6 cut(s) 890, 914, 1078, 1096, 1135, 2400
SmlI CTYRAG 7 cut(s) 500, 1178, 1472, 1918, 2177, 2266, 3151
SmoI CTYRAG 7 cut(s) 500, 1178, 1472, 1918, 2177, 2266, 3151
SphI GCATGC 2 cut(s) 1730, 3533
SsiI CCGC 3 cut(s) 138, 416, 2330
SspI AATATT 5 cut(s) 351, 2110, 2293, 2963, 3463
SspMI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2586, 2864, 3248
StyD4I CCNGG 6 cut(s) 140, 141, 450, 1906, 3336, 3454
StyI CCWWGG 4 cut(s) 295, 331, 1079, 1559
TaaI ACNGT 9 cut(s) 1042, 1288, 1358, 1537, 1598, 2519, 2749, 2853, 3811
TaiI ACGT 3 cut(s) 473, 963, 2789
TaqI TCGA 6 cut(s) 1449, 1893, 2097, 3152, 3312, 3393
TatI WGTACW 5 cut(s) 854, 1532, 1710, 2449, 3277
TauI GCSGC 2 cut(s) 140, 418
TscAI CASTG 9 cut(s) 92, 1293, 1363, 1620, 1768, 2856, 3127, 3196, 3241
TseFI GTSAC 4 cut(s) 1036, 1689, 2513, 3565
TseI GCWGC 2 cut(s) 2239, 3697
Tsp45I GTSAC 4 cut(s) 1036, 1689, 2513, 3565
TspGWI ACGGA 3 cut(s) 32, 311, 2422
TspMI CCCGGG 1 cut(s) 141
TspRI CASTG 9 cut(s) 92, 1293, 1363, 1620, 1768, 2856, 3127, 3196, 3241
Van91I CCANNNNNTGG 1 cut(s) 490
Vha464I CTTAAG 2 cut(s) 1472, 2266
VneI GTGCAC 2 cut(s) 356, 1507
VpaK11BI GGWCC 1 cut(s) 3544
VspI ATTAAT 1 cut(s) 2231
XapI RAATTY 9 cut(s) 389, 660, 672, 712, 1115, 1794, 2156, 2691, 2859
XbaI TCTAGA 1 cut(s) 2863
XceI RCATGY 3 cut(s) 855, 1730, 3533
XhoI CTCGAG 1 cut(s) 3151
XmaI CCCGGG 1 cut(s) 141
XmaJI CCTAGG 2 cut(s) 331, 1559
XmiI GTMKAC 2 cut(s) 576, 1345
XmnI GAANNNNTTC 3 cut(s) 192, 660, 3306
XspI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2586, 2864, 3248
ZraI GACGTC 1 cut(s) 471
ZrmI AGTACT 2 cut(s) 1534, 1712
Zsp2I ATGCAT 5 cut(s) 1093, 1386, 1751, 2128, 2823
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.