RLG00000003598

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003701
Physical Location & Seq
Forward (+)
50508921 .. 50513987
5067 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003598

Sequence Viewer

Length: 4500 bp
ATGGAATTTATTCTTGCAATTGGGAGCGCGATAGTGGGAAAAGTAGCTAGCGTATTGGGACGCCAGGTGGAATATGTAATTTACTGCAAAAGGTACCTTCAAAAAATAGAGGAACAAATGGAAGAATTGAACGCCGTCCAACAGAGGTTAGCTAATGAGGTTTCGGAAGTTAAAACCAGAGGTGAAATAGTTCTACCTGAGGTCCATCTGTGGCTAAAAAACGTGGACGAAATCAATGCAGCGACATACGAATTGTTGGAATATGAAAACCAAGCCAAGCTGAATTGTCTCCATGGGTCTTGTCCAAATCTGATACTCCGTCATCGCCTAGGCAGGAAATCAAAAAAATTGGTGCGCCATATTGATGAAATCTATCAAGGGCGAGGATTTTCCAGAGTTTCAGGTGGTTGGGATGTGTCCACCGGAGAGTTCCTGGCCTTCCCATCAAGGACGTCAATTTTGAAGCAAATCATGGATGAACTGAAAAATCCTAATACCCATAGGATCGGAGTGTATGGAATTCGAGGTGTGGGTAAAACCACACTTGTCCAAGAAGTCTACAGGCAAGCTACAGAAGATAAAAAGTTATTTGATGATGTGGACCTTGTGGTTATCCTACTAGATGTGAAAGAAAATCTACACTTGGAAGGAATTCAAAATAAAATTTTTCGTATGTTAAATATGGAGACTTTCGACAATGGGAATTCAGATGGAAGAGCAAATCGTCCATCTGCCAGTATACAAGATAAAAACATTCTTGTAATTCTGGATAATGTTTGGGAACAAATTGACTTGGAGGCTCTCGAACTTCCAAGTGTGGCTACTTGTAAGATGTTGCTGACATCTAGAAGTAGAAAATCATTATCTGGTATGAGCACGCAGAAAGTTTTTCAGCTTGACATATTACCAGAAGAAGAGGCTTGGGTTCTATTTGAGAAGAAGGCAGGAGATGTTGTTATGCGTCCTGCTATACAAGCAGTAGCAACCCAAGTAGCCAAATGTTGTAGCGGCTTGCCTATTTTAGTTGTCATAGTTGCAAGCGCTTTGAAGAAACAAACTGTTTTACCTCCATGGGAAGATGCATTGAGAAACCTAAGAAAATCTGATAGCCAAGGATTGACAGAGAAGGCATACTTGTGTCTGGAGTGGAGTTACGATCAGCTGAAGGATAAGGAACTTGAGCAGTTGTTCTTGCTATGTGGATTTGCTATATGGGGAAAGGATATTTATTTGCCAGCCTTGCTGAAATATAGTATGGGTTTGGGTCTCTTTCGAAAGGGATACACAATGGAGCAAGCAGAATTTGCATTGCATACAATGGTTGACAAACTTAAAGACTCTTGTCTACTGACAGACGGTGATGATAGCAGATATGTCAGAATGCATGAGCTTGTGCGCAATGTTGCTAACCGGATTGAACTCAGAGATAAACGTATCTTATCATCAGTCGAAGATGGAGGTGACTATGAGTTAAAAGAATGGCTAGACAAGGATTTCTTGAAGAAGTGCACTACGAAATCTTTACAATCCAGTACTGTTCCTGTGCTTTCTGAAGTACCTAGGATATACCCCAAATTAAAGCTGTTTTATTTGCACGGTACAGGTGATATATCAGTAGAAATCCCAACAAACTTTTTTAGAGAGTTTGAGGAACTAGAAGTGTTGGATTTAACGGGACTGCATATGCTGTCACTACCTTCTCTTCAGTTCTTGAGGAATCTGCGTGCCCTATTTTTAGATCAATGCATAGTGGGAGACGTTGCTTTAGTTGGGCAGCTAAGTAATTTAGAAATTCTTAGCTTTTTCCGATCCAATGTCAAACAATTACCAAAGGAAATTGGGAAACTGACCCATCTTCGGTTGTTGGATTTATCTGATTGCTCTGAACTGGAAGTTATTTCACCTGGTGTCATATCAAGCTTGGAGAGACTACAAGAGTTGAGAATGCGAAACAGCTTCAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTATAGAGTTGAAGCATTTGTCTCAGCTATCTGTACTAGAAATACATATTTTGAATGCTGACATTCTTCCAGCAAACTTGTTCTCCCACAAGTTCGAAAGATTCAATATCTTTATTGGGGATGCGTGGGTTGACAGTCATACATCAGGAGAGAAATTTATGGCTGAGACGACCCTCAACACACTAAAACTCAAACTCACTACCAGCGTTGAAAAATTGGATAAAGGATTAATATGGCTGCTGAAGAGATGTGAAGACTTGTCCTTAAGTGTGACAGAGGGCGCTAAAAATATTGAGGCCCATAATTTAGATGCTGATTTTACACATCTCAGTGAGCAGATTGGTTTGCCCAACTTAACAAGATTGGCTGTTCACCGATGCAATGGTTTAAGTTTCTTATTGTCATCTTCCATGGCAAAAGTTTTTGTACAACTTAAACATCTTCGGATATCCAAATGTCAAATCATGAAGGAGATAATACTGACAGAAGGTCACAGTGAAGAAAACAAGGATAACTTGTTTCCGAAGTTGGAACAATTGGAGCTAAATGATCTTCCTTGTCTCACTAGATTCTGTTCAGCAAGTTATGTTGTTGAGTTTGCAGCTCTGGAGAGGTTGCAGTTGGAGAAGTGTACTAAATTGGAGACATTCATCTTTAATCATTCGAGTGACAGAATTCCAATCGACAAAATTAAAGGAAGGGACACGAATGATGAGAATGTTGAGACAGTTGCACCATACTTTCTTTTCAATGAAAAGGTTGGTCTTCCAAGGTTGGAGAGATTGACCATCAATGACCTACCTAGGCTCACAAGAATTTGGCACACCCAACTTGATCCAGACTCTTTTTGCAGACTCAGAGACGTTACAGTTCAACAATGCAATAGTCTTAGAAATATTGTTCCAGACTCAATCATAGCCAGAGTTTTTCAGCAGCTAGAAAAGTTGACAGTAGAGTTCAGCTTGGACATTTGGTACAGTCGACCCTCCCCAGCAGTGTTTTGCTTGAAAGCTGTTCTCCCTGGGATCAGTAGTAGACAAACAGATGGGTTTCTCCCACAATCTTTAAAAACGTTGAGGCTGCATGGAATGGAGATCAAGCTCATGCGTCTCTTGGAAGAGAACCCAGTTTTTCCACTGTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAACCTGGAGTTGTCAGCAATATCCTTCCGGAATCTAACAATTATAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGACAACTTATTTGGTAGCTCAAAGCTTAGTGGGACTAAAAAAATTGAAAGTTGATGAATGTAAAAATATGAAAGAAATCTTCACAAGTGAAGGAATTGAAGAAGATGCAAGTAATTGTGAGATTGTTTTCAGCCGGTTGCAACACTTGGAACTCAGTGATTTAGTAAGTTTGGAACAGTTTTGCTCGAGAAATTGCATTGTGAAGGTCCCATCTTTGGAAAATAGTGCTTTACAAGTAAATTGGTGCCCATTCCAGTTGAAGATTTCCACTGATGGGGTACTAGAACAAGTTGTTTCCTATATGCATCAGGATGTACAAAACTCACATACTGGTAGAGAAGAGGTTCTCGACACAAATGAGTCTGCTTGTAGTCTGGAAATAAATGTTTCTGATATCCCAAACACCTCTGAAGTAGCTCAAGATATTGAGTTTAATGCAGGAGAACTCCATAATGACAGAGGAGAAGAGGTTCTCGACACAACAGAATCTGCTGGTAGTCTGGAAACAAATCAGAAACAGCATGTCCCAAACACCTTTGAAGTAGCTCAAGATATTGAGTTCAATGCAGGAGAACTCCATAATGACAGAGGAGAAAAGTTTCTCGACACAACAGAGTCTGCTTGTAATCTGGAAACAAATAAGAAACAGCATGTCCCAAACACCTCTGAAGTAGCTCAAGACATCGAGTTCAATGCAGGAGAACTCCATGATGACAGACTTGGAAACAACGACGGGCCTCTATTGCCTGGCAATATCGCTGAAAGCGAACATGGAGTCGAAGCTGTCATTCAAGAAACTCCTATCAATTTGCGAAACTTGGAGCATGTTGAGGTTATCAACCGAAATCAGAAGCAAATTGTGACCAATACTCTAAAGAATCTTCAGGAGGCTGATTTTGGCGTGGAAGAGAGCCTCAATGAACCTTTGAATGCAAATGAAGGCAGACCACTTCAATTGAAACAACTTTATGAGGTGCAAACTGATTCTTTGCCGCAAAGCATATCTAAGCTTGATGGGGAGATAAAACGTTTGCAGGATGAATTAAGGGCCAAAGAAGCAGATAGGAGAGATAAACAGCAAAGGCTTGCCGATGAGAGACTGGCGACAGTTGATCGATTGTCTGACAGCTTACCGACGAGAGACTTGCGACAGTTGATCGATTGTCTGACTCTTCAGGAAGCTGACTATCTAGATCGTAGTGCAAAGCTAAGGTTTAGCAAACGACAACAAAGTCAAATGCTGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1500

Amino Acids

170.65

Weight (kDa)

5.3

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 153 - 316 3.4e-24 NB-ARC domain
LRR_14 PF23598 546 - 849 9.4e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 780 - 883 6.5e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 922 - 985 7.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1058 - 1157 2.3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 4482
AatII GACGTC 1 cut(s) 455
Acc16I TGCGCA 1 cut(s) 1397
Acc65I GGTACC 1 cut(s) 93
AccB1I GGYRCC 2 cut(s) 93, 3495
AccI GTMKAC 5 cut(s) 558, 739, 1345, 2980, 3034
AccII CGCG 1 cut(s) 29
AccIII TCCGGA 1 cut(s) 3201
AciI CCGC 2 cut(s) 1008, 4244
AclI AACGTT 2 cut(s) 3071, 4279
AclWI GGATC 4 cut(s) 512, 1803, 2828, 3032
AcuI CTGAAG 8 cut(s) 1184, 1572, 1688, 2261, 3681, 3939, 4118, 4409
AcyI GRCGYC 2 cut(s) 61, 452
AdeI CACNNNGTG 1 cut(s) 1907
AfeI AGCGCT 1 cut(s) 1042
AfiI CCNNNNNNNGG 3 cut(s) 1857, 3466, 3525
AflII CTTAAG 1 cut(s) 2263
AhdI GACNNNNNGTC 1 cut(s) 2487
AjnI CCWGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
AloI GAACNNNNNNTCC 2 cut(s) 3705, 3737
Alw21I GWGCWC 2 cut(s) 878, 1511
Alw44I GTGCAC 1 cut(s) 1507
AlwI GGATC 4 cut(s) 512, 1803, 2828, 3032
AlwNI CAGNNNCTG 2 cut(s) 3740, 3869
Ama87I CYCGRG 1 cut(s) 3436
Aor13HI TCCGGA 1 cut(s) 3201
Aor51HI AGCGCT 1 cut(s) 1042
AoxI GGCC 4 cut(s) 435, 2295, 3986, 4299
ApaLI GTGCAC 1 cut(s) 1507
ApeKI GCWGC 6 cut(s) 239, 1774, 2236, 2600, 2932, 3079
ArsI GACNNNNNNTTYG 6 cut(s) 442, 474, 3244, 3276, 4011, 4043
AseI ATTAAT 1 cut(s) 2228
Asp700I GAANNNNTTC 6 cut(s) 9, 189, 651, 3594, 3720, 3849
Asp718I GGTACC 1 cut(s) 93
AspA2I CCTAGG 3 cut(s) 328, 1559, 2801
AspLEI GCGC 5 cut(s) 29, 357, 1043, 1398, 2282
AspS9I GGNCC 6 cut(s) 202, 601, 2296, 3457, 3986, 4299
AsuHPI GGTGA 8 cut(s) 194, 1370, 1472, 1616, 1893, 1991, 2363, 3166
AsuII TTCGAA 2 cut(s) 1273, 2094
AsuNHI GCTAGC 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 3436
AvaII GGWCC 3 cut(s) 202, 601, 3457
AvrII CCTAGG 3 cut(s) 328, 1559, 2801
AxyI CCTNAGG 1 cut(s) 198
BaeGI GKGCMC 3 cut(s) 1511, 1729, 3500
BanI GGYRCC 2 cut(s) 93, 3495
BbsI GAAGAC 2 cut(s) 2259, 2756
Bbv12I GWGCWC 2 cut(s) 878, 1511
BbvI GCAGC 6 cut(s) 251, 1786, 2223, 2612, 2944, 3066
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 6 cut(s) 218, 252, 3926, 3960, 4378, 4412
BciT130I CCWGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
BciVI GTATCC 1 cut(s) 1274
BfmI CTRYAG 2 cut(s) 559, 570
BfoI RGCGCY 2 cut(s) 1044, 2283
BfrI CTTAAG 1 cut(s) 2263
BfuI GTATCC 1 cut(s) 1274
BisI GCNGC 8 cut(s) 240, 1009, 1775, 2237, 2601, 2933, 3080, 4244
BlnI CCTAGG 3 cut(s) 328, 1559, 2801
BlsI GCNGC 8 cut(s) 241, 1010, 1776, 2238, 2602, 2934, 3081, 4245
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
Bme18I GGWCC 3 cut(s) 202, 601, 3457
BmeRI GACNNNNNGTC 1 cut(s) 2487
BmeT110I CYCGRG 1 cut(s) 3436
BmgT120I GGNCC 6 cut(s) 202, 601, 2296, 3457, 3986, 4299
BmiI GGNNCC 3 cut(s) 95, 3459, 3497
BmrFI CCNGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
BmrI ACTGGG 1 cut(s) 3119
BmsI GCATC 6 cut(s) 1069, 2110, 2299, 2366, 3346, 3565
BmtI GCTAGC 1 cut(s) 51
BmuI ACTGGG 1 cut(s) 3119
BoxI GACNNNNGTC 1 cut(s) 1341
BpiI GAAGAC 2 cut(s) 2259, 2756
BpmI CTGGAG 3 cut(s) 1163, 2627, 3200
Bpu10I CCTNAGC 1 cut(s) 4460
Bpu14I TTCGAA 2 cut(s) 1273, 2094
BpuEI CTTGAG 5 cut(s) 1199, 1732, 3654, 3783, 3912
Bsa29I ATCGAT 2 cut(s) 4366, 4410
BsaHI GRCGYC 2 cut(s) 61, 452
BsaI GGTCTC 1 cut(s) 1271
BsaWI WCCGGW 3 cut(s) 422, 1410, 3201
BsaXI ACNNNNNCTCC 6 cut(s) 939, 969, 2066, 2096, 3705, 3735
Bsc4I CCNNNNNNNGG 3 cut(s) 1857, 3466, 3525
Bse118I RCCGGY 1 cut(s) 3384
Bse1I ACTGG 7 cut(s) 735, 1530, 1893, 3125, 3505, 3586, 4356
Bse21I CCTNAGG 1 cut(s) 198
Bse3DI GCAATG 3 cut(s) 1307, 1405, 2386
BseAI TCCGGA 1 cut(s) 3201
BseBI CCWGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
BseCI ATCGAT 2 cut(s) 4366, 4410
BseGI GGATG 5 cut(s) 418, 481, 2125, 3568, 4294
BseLI CCNNNNNNNGG 3 cut(s) 1857, 3466, 3525
BseMI GCAATG 3 cut(s) 1307, 1405, 2386
BseMII CTCAG 7 cut(s) 189, 1435, 2036, 2154, 2341, 2869, 3418
BseNI ACTGG 7 cut(s) 735, 1530, 1893, 3125, 3505, 3586, 4356
BseRI GAGGAG 2 cut(s) 3726, 3855
BseSI GKGCMC 3 cut(s) 1511, 1729, 3500
BseXI GCAGC 6 cut(s) 251, 1786, 2223, 2612, 2944, 3066
BseYI CCCAGC 1 cut(s) 2989
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 4 cut(s) 437, 2297, 3988, 4301
BshNI GGYRCC 2 cut(s) 93, 3495
BshVI ATCGAT 2 cut(s) 4366, 4410
BsiHKAI GWGCWC 2 cut(s) 878, 1511
BsiHKCI CYCGRG 1 cut(s) 3436
BsiSI CCGG 4 cut(s) 423, 1411, 3202, 3385
BslFI GGGAC 7 cut(s) 72, 1689, 2714, 3297, 3443, 3761, 3890
BslI CCNNNNNNNGG 3 cut(s) 1857, 3466, 3525
BsmBI CGTCTC 4 cut(s) 1749, 2159, 2853, 3113
BsmFI GGGAC 7 cut(s) 72, 1689, 2714, 3297, 3443, 3761, 3890
BsmI GAATGC 4 cut(s) 1386, 1950, 2059, 4186
BsnI GGCC 4 cut(s) 437, 2297, 3988, 4301
Bso31I GGTCTC 1 cut(s) 1271
BsoBI CYCGRG 1 cut(s) 3436
Bsp119I TTCGAA 2 cut(s) 1273, 2094
Bsp1286I GDGCHC 4 cut(s) 878, 1511, 1729, 3500
Bsp13I TCCGGA 1 cut(s) 3201
Bsp1407I TGTACA 2 cut(s) 2425, 3565
Bsp19I CCATGG 3 cut(s) 292, 1070, 2409
BspACI CCGC 2 cut(s) 1008, 4244
BspANI GGCC 4 cut(s) 437, 2297, 3988, 4301
BspCNI CTCAG 7 cut(s) 190, 1434, 2035, 2155, 2340, 2868, 3417
BspDI ATCGAT 2 cut(s) 4366, 4410
BspEI TCCGGA 1 cut(s) 3201
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 2463
BspLI GGNNCC 3 cut(s) 95, 3459, 3497
BspOI GCTAGC 1 cut(s) 51
BspPI GGATC 4 cut(s) 512, 1803, 2828, 3032
BspQI GCTCTTC 1 cut(s) 709
BspT104I TTCGAA 2 cut(s) 1273, 2094
BspT107I GGYRCC 2 cut(s) 93, 3495
BspTI CTTAAG 1 cut(s) 2263
BspTNI GGTCTC 1 cut(s) 1271
BsrDI GCAATG 3 cut(s) 1307, 1405, 2386
BsrFI RCCGGY 1 cut(s) 3384
BsrGI TGTACA 2 cut(s) 2425, 3565
BsrI ACTGG 7 cut(s) 735, 1530, 1893, 3125, 3505, 3586, 4356
BssAI RCCGGY 1 cut(s) 3384
BssNAI GTATAC 1 cut(s) 740
BssNI GRCGYC 2 cut(s) 61, 452
BssT1I CCWWGG 8 cut(s) 292, 328, 1070, 1111, 1559, 2409, 2768, 2801
Bst1107I GTATAC 1 cut(s) 740
Bst2UI CCWGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
Bst6I CTCTTC 9 cut(s) 709, 909, 1707, 2237, 3111, 3585, 3711, 4152, 4428
BstACI GRCGYC 2 cut(s) 61, 452
BstAFI CTTAAG 1 cut(s) 2263
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstAUI TGTACA 2 cut(s) 2425, 3565
BstBI TTCGAA 2 cut(s) 1273, 2094
BstDSI CCRYGG 3 cut(s) 292, 1070, 2409
BstF5I GGATG 5 cut(s) 418, 481, 2125, 3568, 4294
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 2 cut(s) 1044, 2283
BstHHI GCGC 5 cut(s) 29, 357, 1043, 1398, 2282
BstMWI GCNNNNNNNGC 5 cut(s) 974, 1240, 1304, 3994, 4307
BstNI CCWGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
BstNSI RCATGY 3 cut(s) 3776, 3905, 4079
BstPAI GACNNNNGTC 1 cut(s) 1341
BstSCI CCNGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
BstSFI CTRYAG 2 cut(s) 559, 570
BstSLI GKGCMC 3 cut(s) 1511, 1729, 3500
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 6 cut(s) 251, 1786, 2223, 2612, 2944, 3066
BstV2I GAAGAC 2 cut(s) 2259, 2756
BstZ17I GTATAC 1 cut(s) 740
Bsu15I ATCGAT 2 cut(s) 4366, 4410
Bsu36I CCTNAGG 1 cut(s) 198
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 4 cut(s) 437, 2297, 3988, 4301
BsuTUI ATCGAT 2 cut(s) 4366, 4410
BtgI CCRYGG 3 cut(s) 292, 1070, 2409
BtgZI GCGATG 1 cut(s) 308
BtsCI GGATG 5 cut(s) 418, 481, 2125, 3568, 4294
BtsI GCAGTG 1 cut(s) 3000
BtsIMutI CAGTG 6 cut(s) 2335, 2500, 3000, 3134, 3412, 3519
CaiI CAGNNNCTG 2 cut(s) 3740, 3869
CciI TCATGA 1 cut(s) 2463
CfoI GCGC 5 cut(s) 29, 357, 1043, 1398, 2282
Cfr10I RCCGGY 1 cut(s) 3384
Cfr13I GGNCC 6 cut(s) 202, 601, 2296, 3457, 3986, 4299
ClaI ATCGAT 2 cut(s) 4366, 4410
CseI GACGC 3 cut(s) 69, 950, 3095
CsiI ACCWGGT 1 cut(s) 1903
CspCI CAANNNNNGTGG 2 cut(s) 4188, 4223
DraI TTTAAA 1 cut(s) 3066
DraIII CACNNNGTG 1 cut(s) 1907
DrdI GACNNNNNNGTC 1 cut(s) 4482
DriI GACNNNNNGTC 1 cut(s) 2487
DseDI GACNNNNNNGTC 1 cut(s) 4482
Eam1104I CTCTTC 9 cut(s) 709, 909, 1707, 2237, 3111, 3585, 3711, 4152, 4428
Eam1105I GACNNNNNGTC 1 cut(s) 2487
EarI CTCTTC 9 cut(s) 709, 909, 1707, 2237, 3111, 3585, 3711, 4152, 4428
Eco130I CCWWGG 8 cut(s) 292, 328, 1070, 1111, 1559, 2409, 2768, 2801
Eco31I GGTCTC 1 cut(s) 1271
Eco32I GATATC 2 cut(s) 2448, 3646
Eco47I GGWCC 3 cut(s) 202, 601, 3457
Eco47III AGCGCT 1 cut(s) 1042
Eco57I CTGAAG 8 cut(s) 1184, 1572, 1688, 2261, 3681, 3939, 4118, 4409
Eco81I CCTNAGG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 3436
EcoO109I RGGNCCY 1 cut(s) 3457
EcoRI GAATTC 4 cut(s) 519, 651, 703, 2673
EcoRII CCWGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
EcoRV GATATC 2 cut(s) 2448, 3646
EcoT14I CCWWGG 8 cut(s) 292, 328, 1070, 1111, 1559, 2409, 2768, 2801
EcoT22I ATGCAT 4 cut(s) 1084, 1386, 1748, 3558
ErhI CCWWGG 8 cut(s) 292, 328, 1070, 1111, 1559, 2409, 2768, 2801
Esp3I CGTCTC 4 cut(s) 1749, 2159, 2853, 3113
FalI AAGNNNNNCTT 6 cut(s) 1118, 1150, 1481, 1513, 2498, 2530
FaqI GGGAC 7 cut(s) 72, 1689, 2714, 3297, 3443, 3761, 3890
FauNDI CATATG 1 cut(s) 1683
FblI GTMKAC 5 cut(s) 558, 739, 1345, 2980, 3034
Fnu4HI GCNGC 8 cut(s) 240, 1009, 1775, 2237, 2601, 2933, 3080, 4244
FokI GGATG 5 cut(s) 425, 488, 2132, 3575, 4301
Fsp4HI GCNGC 8 cut(s) 240, 1009, 1775, 2237, 2601, 2933, 3080, 4244
FspI TGCGCA 1 cut(s) 1397
GlaI GCGC 5 cut(s) 28, 356, 1042, 1397, 2281
GluI GCNGC 8 cut(s) 240, 1009, 1775, 2237, 2601, 2933, 3080, 4244
GsaI CCCAGC 1 cut(s) 2993
GsuI CTGGAG 3 cut(s) 1163, 2627, 3200
HaeII RGCGCY 2 cut(s) 1044, 2283
HaeIII GGCC 4 cut(s) 437, 2297, 3988, 4301
HapII CCGG 4 cut(s) 423, 1411, 3202, 3385
HgaI GACGC 3 cut(s) 69, 950, 3095
HhaI GCGC 5 cut(s) 29, 357, 1043, 1398, 2282
Hin1I GRCGYC 2 cut(s) 61, 452
Hin6I GCGC 5 cut(s) 27, 355, 1041, 1396, 2280
HinP1I GCGC 5 cut(s) 27, 355, 1041, 1396, 2280
HincII GTYRAC 4 cut(s) 1324, 2131, 2946, 2981
HindII GTYRAC 4 cut(s) 1324, 2131, 2946, 2981
HindIII AAGCTT 3 cut(s) 1918, 3274, 4259
HpaII CCGG 4 cut(s) 423, 1411, 3202, 3385
HphI GGTGA 8 cut(s) 194, 1370, 1472, 1616, 1893, 1991, 2363, 3166
Hpy99I CGWCG 2 cut(s) 3986, 4390
HpyCH4IV ACGT 7 cut(s) 222, 452, 1432, 1758, 2862, 3071, 4279
HpyF10VI GCNNNNNNNGC 5 cut(s) 974, 1240, 1304, 3994, 4307
HpySE526I ACGT 7 cut(s) 222, 452, 1432, 1758, 2862, 3071, 4279
Hsp92I GRCGYC 2 cut(s) 61, 452
HspAI GCGC 5 cut(s) 27, 355, 1041, 1396, 2280
Kpn2I TCCGGA 1 cut(s) 3201
KpnI GGTACC 1 cut(s) 97
LguI GCTCTTC 1 cut(s) 709
LmnI GCTCC 4 cut(s) 24, 1291, 2539, 4072
Lsp1109I GCAGC 6 cut(s) 251, 1786, 2223, 2612, 2944, 3066
LweI GCATC 6 cut(s) 1069, 2110, 2299, 2366, 3346, 3565
MabI ACCWGGT 1 cut(s) 1903
MaeII ACGT 7 cut(s) 222, 452, 1432, 1758, 2862, 3071, 4279
MaeIII GTNAC 9 cut(s) 1151, 1460, 1689, 2269, 2489, 2666, 2863, 3223, 4111
MfeI CAATTG 3 cut(s) 18, 2534, 4205
MhlI GDGCHC 4 cut(s) 878, 1511, 1729, 3500
MlyI GAGTC 8 cut(s) 1331, 2834, 2847, 2900, 3620, 3875, 4035, 4414
MmeI TCCRAC 8 cut(s) 163, 237, 1644, 1845, 2508, 2601, 2754, 3120
Mph1103I ATGCAT 4 cut(s) 1084, 1386, 1748, 3558
MroI TCCGGA 1 cut(s) 3201
MroXI GAANNNNTTC 6 cut(s) 9, 189, 651, 3594, 3720, 3849
MslI CAYNNNNRTG 5 cut(s) 363, 1135, 2328, 2664, 3561
MspA1I CMGCKG 1 cut(s) 1162
MspCI CTTAAG 1 cut(s) 2263
MspI CCGG 4 cut(s) 423, 1411, 3202, 3385
MspR9I CCNGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
MunI CAATTG 3 cut(s) 18, 2534, 4205
Mva1269I GAATGC 4 cut(s) 1386, 1950, 2059, 4186
MvaI CCWGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 5 cut(s) 974, 1240, 1304, 3994, 4307
NcoI CCATGG 3 cut(s) 292, 1070, 2409
NdeI CATATG 1 cut(s) 1683
NheI GCTAGC 1 cut(s) 47
NlaIV GGNNCC 3 cut(s) 95, 3459, 3497
NmuCI GTSAC 6 cut(s) 1460, 1689, 2269, 2489, 2666, 4111
NsbI TGCGCA 1 cut(s) 1397
NsiI ATGCAT 4 cut(s) 1084, 1386, 1748, 3558
NspI RCATGY 3 cut(s) 3776, 3905, 4079
NspV TTCGAA 2 cut(s) 1273, 2094
PaeR7I CTCGAG 1 cut(s) 3436
PagI TCATGA 1 cut(s) 2463
PasI CCCWGGG 1 cut(s) 3020
PciSI GCTCTTC 1 cut(s) 709
PcsI WCGNNNNNNNCGW 1 cut(s) 4014
PctI GAATGC 4 cut(s) 1386, 1950, 2059, 4186
PdmI GAANNNNTTC 6 cut(s) 9, 189, 651, 3594, 3720, 3849
PfeI GAWTC 7 cut(s) 1717, 2100, 2568, 3205, 3737, 4129, 4235
PkrI GCNGC 8 cut(s) 241, 1010, 1776, 2238, 2602, 2934, 3081, 4245
PleI GAGTC 8 cut(s) 1331, 2834, 2847, 2900, 3619, 3874, 4034, 4414
PpsI GAGTC 8 cut(s) 1331, 2834, 2847, 2900, 3619, 3874, 4034, 4414
PpuMI RGGWCCY 1 cut(s) 3457
PshAI GACNNNNGTC 1 cut(s) 1341
PshBI ATTAAT 1 cut(s) 2228
Psp1406I AACGTT 2 cut(s) 3071, 4279
Psp5II RGGWCCY 1 cut(s) 3457
Psp6I CCWGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
PspFI CCCAGC 1 cut(s) 2989
PspGI CCWGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
PspN4I GGNNCC 3 cut(s) 95, 3459, 3497
PspPI GGNCC 6 cut(s) 202, 601, 2296, 3457, 3986, 4299
PspPPI RGGWCCY 1 cut(s) 3457
PstNI CAGNNNCTG 2 cut(s) 3740, 3869
PvuII CAGCTG 1 cut(s) 1162
RseI CAYNNNNRTG 5 cut(s) 363, 1135, 2328, 2664, 3561
SalI GTCGAC 1 cut(s) 2979
SapI GCTCTTC 1 cut(s) 709
SatI GCNGC 8 cut(s) 240, 1009, 1775, 2237, 2601, 2933, 3080, 4244
Sau96I GGNCC 6 cut(s) 202, 601, 2296, 3457, 3986, 4299
ScaI AGTACT 1 cut(s) 1534
SchI GAGTC 8 cut(s) 1331, 2834, 2847, 2900, 3620, 3875, 4035, 4414
ScrFI CCNGG 6 cut(s) 65, 434, 1905, 3021, 3179, 3999
SduI GDGCHC 4 cut(s) 878, 1511, 1729, 3500
SexAI ACCWGGT 1 cut(s) 1903
SfaNI GCATC 6 cut(s) 1069, 2110, 2299, 2366, 3346, 3565
SfcI CTRYAG 2 cut(s) 559, 570
Sfr274I CTCGAG 1 cut(s) 3436
SfuI TTCGAA 2 cut(s) 1273, 2094
SinI GGWCC 3 cut(s) 202, 601, 3457
SlaI CTCGAG 1 cut(s) 3436
SmiMI CAYNNNNRTG 5 cut(s) 363, 1135, 2328, 2664, 3561
SmlI CTYRAG 7 cut(s) 1178, 1711, 2263, 3436, 3669, 3798, 3927
SmoI CTYRAG 7 cut(s) 1178, 1711, 2263, 3436, 3669, 3798, 3927
SsiI CCGC 2 cut(s) 1008, 4244
SspI AATATT 3 cut(s) 2290, 2896, 3248
StyD4I CCNGG 6 cut(s) 63, 432, 1903, 3019, 3177, 3997
StyI CCWWGG 8 cut(s) 292, 328, 1070, 1111, 1559, 2409, 2768, 2801
TaiI ACGT 7 cut(s) 225, 455, 1435, 1761, 2865, 3074, 4282
TatI WGTACW 5 cut(s) 1532, 2032, 2425, 2630, 3565
TauI GCSGC 2 cut(s) 1011, 4246
TfiI GAWTC 7 cut(s) 1717, 2100, 2568, 3205, 3737, 4129, 4235
TscAI CASTG 6 cut(s) 2335, 2500, 3000, 3141, 3412, 3526
TseFI GTSAC 6 cut(s) 1460, 1689, 2269, 2489, 2666, 4111
TseI GCWGC 6 cut(s) 239, 1774, 2236, 2600, 2932, 3079
Tsp45I GTSAC 6 cut(s) 1460, 1689, 2269, 2489, 2666, 4111
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 6 cut(s) 2335, 2500, 3000, 3141, 3412, 3526
Vha464I CTTAAG 1 cut(s) 2263
VneI GTGCAC 1 cut(s) 1507
VpaK11BI GGWCC 3 cut(s) 202, 601, 3457
VspI ATTAAT 1 cut(s) 2228
XbaI TCTAGA 3 cut(s) 845, 3148, 4441
XceI RCATGY 3 cut(s) 3776, 3905, 4079
XhoI CTCGAG 1 cut(s) 3436
XmaJI CCTAGG 3 cut(s) 328, 1559, 2801
XmiI GTMKAC 5 cut(s) 558, 739, 1345, 2980, 3034
XmnI GAANNNNTTC 6 cut(s) 9, 189, 651, 3594, 3720, 3849
ZraI GACGTC 1 cut(s) 453
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 4 cut(s) 1084, 1386, 1748, 3558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.