Rroxscaffold_3G00224900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003701
Physical Location & Seq
Reverse (-)
8165502 .. 8172740
7239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224900.1

Sequence Viewer

Length: 4341 bp
ATGAACTCGAACAAGGAGCTGCTTTGTTTAATTTTCTTTCGTGGATTGAAGAATGAAGGTTGCGGACTGGAAATGAGGAAATCAGGAATCCAGCCCGTCCAAGGTTCCAATCCTGGATCCGCCACTGGAGATGATGCAGCACCAAATGAGATTACTTTCACCCGCTTGGGACAGTTAAAACTTTATGATCTACCAAGTCTACGAGGATTTTGCTCTTCCAGAAATTGCATTGTCAAATTCCCATCCCCGTGCACCTTAATGGTAGAACTCTGCCCAATCGAGATGAAGATTTCCCCCGATGGGTTCCTCCTGCAGGCAGCATCTGATATAAAATGGAGTTATTATCAGTTGAATGATGATCTTAAGCCATTGTTCCTGCTCTGTGGGATTTCTATGAGGGGAAACTATATTAGCTTCGAAGACTTGTTCAGGTATAGTATGGGTTTGGGTTTCTTTGGAAAGGCCTTCAAAGTGGAGGAAGCACGAGATGTATTGCATTTACGGATCAAAAAGCTTAAAGATTTTCATCTACTGACTGATAGCAGCGATAATAGATATGTAAGAATGCCTGACCATGTACGTGATGTTGCGAACCAGATTGCGTTAAGAGATCAACACATCTTATCATTAGTGGAAGACGGTCACCTCCATGAGTTGAAAGAATGGACAGATAAGGATTTCTTAGAGAAGTGCACAATCATGTCTTTCCCCTCTAGCAACAATATCCCATGGCTTCCTGAAGTTATGCACTGCCCAGCTCTGAAAATGTTTCTTTTGCAAGGGGATCTAGATGATGGCTCGCAGAAAATCTCACCTAGATTTTTTGAAAAGATGAAAAACCTCCGAGTGTTGAGTTTGCGATGTATGACTATTCCATCACTACCTCCGTCTTTTCAGTCCCTGAAAAATCTGGATTCCCTGTGTTTAGATAAGTGCATATTGGGAGACGTCGCTTTAATTGGGGAGTTAAGTAACTTAGAAGTTCTTTCCTTTTCAGAATCCAATATCAAACAATTGCCTAGAGAAGTAGGGAAATTGACTCATCTTCGGTTGTTGGATTTATCTGATTGCTCTAATCTTGAAGTGATTTCACCTGGTGTTATTTCAAGCCTAGGGAGATTGGAAGAGTTGAGAATGAGAAATAGCTTTAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCAAGCCTCTCAGAGCTGAAGCATTTGTCTCAGCTATCTGCATTAGAAATACATATTCCTAATGCTGACATTTTTCCAGCAAACTTGTTCTCCCACAAGTTAGAAAGATTCAATATAATTATTGGGGATACATATACGTGGAAGAAACATATGACTGAGACGACCCTCAACACACTAAAAGTGAAGCTCACTACTGCTAGCATTGAAGAATTGGATGAAGGCCTAAAATTTCTTTTGAAAAGATCAGAAGATTTGTCCTTGTATGCGGAGGGCATGAATAACATTGTCCATTCACTGGACACTCAAGATTTTGAGCATCTGAAACATCTGCAACTCCAAAGCAACATTGACTTTAATCATATTATAAACAAGAAGCAGCCTCGATCAAGCTTCGACGGCGCGGCACTGCTTGCCGGACGGAGGCTAGGGGTAGGACGGACCGGTCTGCAGTCGGATGGAGTCGCCCTCGATGAGGTCGCAGCTCTGCCCAGAAAGTTGCAGTTGCAGGTCGAGTCGCTGCCCAAAACCTGCAACCTTGACCTCCTCGACCTCGATTGCTGCCCGAACCGTACGGGATGCCTCCGGCCTCCGGCCTCCGTCCGCCAAGCCCCGAGCAGCTGTCGCCGCCTGGAACGCCGCTGCTGCGTCGCCGTCCGTACTTTAGCGAATCTTGCGGAACTCAAACATCTTGAGATATCCACATGTGAAATTATGGAAGAGAGTCTATCATCAACAAGAGAATACGGCCAAGAAATTATGGATTACATGTTTTATAAGCGGGAAGATTTGAAGTTAAATGAATTGCCAAACATCACCAGATTTTGTTCAGGAAGCATAGAAAAATTGGACACAGAAGGTTGGATTAAACTTGGAGCTTATAATGCAGCTATGAATAGAAATACGAACAAAATTGAAGAGACTCACTCGGAAGAGAAGCTTGATATTACGAACGAGTTGGTTACTATAAAATACTTCCTCTTTGACCTAAAGGCTGTGTTCCCGAAGTTAACAACCTTGACAGTACATGAGTGTGATCATCTAAGCTTCTTGTTCACAATTTCTATGGCTAAAAGTCTTGTGGAACTCAAACATCTCGAGATATCCACATGTGAAATCATAGAAGAGATTGTTTCATCAACAAGTGAATACTGTCAAGAAAATATGGAGGACATGTTTCCTAAGCTGGAAGAATTGAAGCTAAATAAACTTCCAAACCTCACCAGATTTTGCTCAGGAAGTTATATTCAACTTCCGTTCTTAGAAAAGTTGGATATACAAGGCTGTACCAAACTGGCCGCATTCATTTCTGACAATATTGCTATCAGTGGTAAAAATACTAGAATCTACAAAGAAATTGAAGAGAAGGACTCCGAAGGGAACAACTCGTTGAATACTGTACAATACTTCCTCTTTGACGAAAAGGTGGGTTTGCCTAACTTGACAAGCTTGATGGTTCATAGATGTGATGGTTTACGTTTCTTATCATCCATGGCAAGAAGTCTTGTACAACTCAAACATCTTGTGATATCTGAATGTCAAATCATGGAAGTAGTATTGACCGAAGTATACAATGAAGAGAACAAGGTTAACATGCTTCCTAAGTTAGAACAATTGAAGCTAAACGATCTTCCTTTTCTCAATAGATTCTGCTCTGGAAGTTTTGTTGAATTTGCATCTATGGAAGGATTGCATTTAGAGAAGTGTCCTAAATTGGGGACATTCATCTGTAATCCTTTGAGTAACAGTATTGCAAGCTCCAAAGAAGTAGAAGAAAGGAACAGGGATCAGAACATTGAGACAGTTGCACCATACTTTCTCTTTGACAAAAAGGTGTGGTTTCCAAAGTTGGAGAGATTGATCATCCGTGACCTACCTAAGCTTAAAACAATTTGGCACACCCAGCTTGCTCCAGACTCTTTTTGCAAACTCCGAGACGTTACACTTCACCAGTGCAATAGTCTTAAAGCTATTCTTCCAGACTCATTGATAGCCGGTGTTTTTCAGCAGCTACAAAAGTTGACAGTGGATGAATGTGGAGTGGAGGAAATAATTTTTGAGAAAGTGGATATTTTTGCAGCCGAATTTTCAGGCCTTCAGAAAACACATGACTTGAGCAGTTTGCATAAACCTGCAGCTAAACTGCTCACTCCAGCTAATCAATCTTCTATTCTAATTAACGAGAAATCATTCCCCAAACTGGAAGATTTGACTTTTGGCCCGCGCCCCATGGATATTTGGTACGGTCGACCCACCTCAGCAGTGTGCTGTGAGAAAGCTGTTTTCGCTGGGATCGGTAATAGACATACAGATGGGTCCCTCCCACATTTGAGAACGTTGAGGCTGCATGGAATGGAGACGCTTATGCGTCTCTGGGAGACCCAACCAGCTGGCCAAGATTTTCCACAGTTGAAAATTCTAGAGATGAAGTGTTGTGGATTGAATAATGTAGAGTTGTCTTCAGTATCCTTCGAGAATCTAACAATTTTAGAAGTAACCTTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCGAAGTTTAAGGCAACTCAAAAAACTGAAAGTTGATGAATGTAAAAGTATGAAACAAATCTTGGCAAGTGAAGGAATCGAGGAAGATGACAGTAATTGTGAGATTGTTTTCAGCCAGTTGCAACATTTGGAACTCAGTGATTTAATAAGTTTGGAATGCTTTTGCTCGAGCAATTGCACTGTGAAGGTTCCAAACTTGGAAACTTTAGAGGTAAAATGGTGCTCGATCGAGTTGAAGGTTTCCTCTGATATACTAGAAGTTGATTCCAATATGCATCTGGATGTACAAAAGTCACATTCTAGTAAAGAGGCTTTCAATACCACTGAGCCTGCTTGTAGCTCGGGAAAAACTCAGAAAAAACCTGTCACAAACTCCTCTGAAGAAGCTGAACATATTGAGTTTAATGCACGGGAACTCCCTGATGATAGCCTTATTGAACGTTTGAAGGAAGAATTAAGGGCCAGTCAAGCACACATCAGACTTATGGTTCTGAGCTTGGTGCTATTCCCGGGTAGCCAGTTATCTGGTGGTTCTTTGTATTGTGTACGAAGTGAGTACTCTGTTTCTCCTGTGTTATTTGATGAGTCTTGTGTGTGGGTTTTGAGTTTATGGGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1446

Amino Acids

164.06

Weight (kDa)

5.59

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 240 - 507 2e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 723 - 831 5e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 871 - 967 4.6e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1006 - 1073 9.8e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1010 - 1073 2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1210 - 1315 3.4e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1547, 1956, 2061
AasI GACNNNNNNGTC 1 cut(s) 1623
AatII GACGTC 1 cut(s) 951
Acc36I ACCTGC 3 cut(s) 1678, 1718, 3319
AccB7I CCANNNNNTGG 1 cut(s) 1477
AccI GTMKAC 3 cut(s) 199, 2748, 3427
AccII CGCG 2 cut(s) 1583, 3403
AclI AACGTT 2 cut(s) 3515, 4159
AclWI GGATC 6 cut(s) 111, 124, 512, 792, 2973, 3479
AcoI YGGCCR 3 cut(s) 1927, 2475, 3571
AcsI RAATTY 5 cut(s) 236, 1409, 2849, 3263, 3594
AcuI CTGAAG 5 cut(s) 759, 1220, 3260, 3624, 4119
AcyI GRCGYC 1 cut(s) 948
AdeI CACNNNGTG 1 cut(s) 1097
AflII CTTAAG 1 cut(s) 362
AflIII ACRYGT 4 cut(s) 1883, 1947, 2288, 2352
AgeI ACCGGT 1 cut(s) 1622
AjnI CCWGG 3 cut(s) 112, 1093, 1809
AjuI GAANNNNNNNTTGG 2 cut(s) 3764, 3796
Alw21I GWGCWC 3 cut(s) 254, 695, 3944
Alw26I GTCTC 9 cut(s) 939, 1215, 1334, 2093, 2972, 3108, 3530, 3551, 3554
Alw44I GTGCAC 2 cut(s) 250, 691
AlwI GGATC 6 cut(s) 111, 124, 512, 792, 2973, 3479
AlwNI CAGNNNCTG 2 cut(s) 323, 901
Ama87I CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
ApaLI GTGCAC 2 cut(s) 250, 691
ApoI RAATTY 5 cut(s) 236, 1409, 2849, 3263, 3594
ArsI GACNNNNNNTTYG 2 cut(s) 3694, 3726
AsiGI ACCGGT 1 cut(s) 1622
Asp700I GAANNNNTTC 1 cut(s) 3368
AspA2I CCTAGG 1 cut(s) 1111
AspLEI GCGC 2 cut(s) 1583, 3405
AspS9I GGNCC 4 cut(s) 1620, 3398, 3495, 4179
AsuC2I CCSGG 2 cut(s) 4229, 4230
AsuHPI GGTGA 8 cut(s) 151, 635, 804, 1083, 1181, 1987, 2392, 3119
AsuII TTCGAA 1 cut(s) 417
AsuNHI GCTAGC 1 cut(s) 1379
AvaI CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
AvaII GGWCC 2 cut(s) 1620, 3495
AvrII CCTAGG 1 cut(s) 1111
BaeGI GKGCMC 2 cut(s) 254, 695
BalI TGGCCA 1 cut(s) 3573
BamHI GGATCC 1 cut(s) 116
BauI CACGAG 1 cut(s) 483
BbsI GAAGAC 3 cut(s) 426, 642, 3630
Bbv12I GWGCWC 3 cut(s) 254, 695, 3944
BbvCI CCTCAGC 1 cut(s) 3436
BccI CCATC 8 cut(s) 250, 293, 788, 883, 1631, 2626, 2642, 3485
BceAI ACGGC 3 cut(s) 1594, 1817, 1942
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciT130I CCWGG 3 cut(s) 114, 1095, 1811
BciVI GTATCC 2 cut(s) 1303, 3655
BclI TGATCA 2 cut(s) 2215, 3039
BcnI CCSGG 2 cut(s) 4229, 4230
BcoDI GTCTC 9 cut(s) 939, 1215, 1334, 2093, 2972, 3108, 3530, 3551, 3554
BfmI CTRYAG 3 cut(s) 311, 1628, 3312
BfrI CTTAAG 1 cut(s) 362
BfuAI ACCTGC 3 cut(s) 1678, 1718, 3319
BfuI GTATCC 2 cut(s) 1303, 3655
BlnI CCTAGG 1 cut(s) 1111
BmcAI AGTACT 1 cut(s) 4277
Bme1390I CCNGG 5 cut(s) 114, 1095, 1811, 4229, 4230
Bme18I GGWCC 2 cut(s) 1620, 3495
BmeT110I CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
BmgT120I GGNCC 4 cut(s) 1620, 3398, 3495, 4179
BmiI GGNNCC 6 cut(s) 106, 118, 305, 3496, 3497, 3909
BmrFI CCNGG 5 cut(s) 114, 1095, 1811, 4229, 4230
BmsI GCATC 6 cut(s) 124, 329, 1507, 1748, 2864, 4003
BmtI GCTAGC 1 cut(s) 1383
BpiI GAAGAC 3 cut(s) 426, 642, 3630
BplI GAGNNNNNCTC 6 cut(s) 1632, 1664, 2090, 2122, 2534, 2566
BpmI CTGGAG 3 cut(s) 147, 3075, 3315
Bpu10I CCTNAGC 4 cut(s) 2361, 2413, 3057, 3436
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 3 cut(s) 1470, 1892, 3313
BpuMI CCSGG 2 cut(s) 4229, 4230
BsaAI YACGTR 2 cut(s) 581, 1320
BsaBI GATNNNNATC 1 cut(s) 525
BsaHI GRCGYC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 3551
BsaJI CCNNGG 6 cut(s) 100, 728, 1111, 2670, 3408, 4228
BsaWI WCCGGW 1 cut(s) 1622
BsaXI ACNNNNNCTCC 6 cut(s) 1256, 1286, 2339, 2369, 4119, 4149
Bse118I RCCGGY 2 cut(s) 1622, 3173
Bse1I ACTGG 9 cut(s) 72, 130, 1482, 2478, 3130, 3384, 3835, 4182, 4237
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 3 cut(s) 114, 1095, 1811
BseDI CCNNGG 6 cut(s) 100, 728, 1111, 2670, 3408, 4228
BseGI GGATG 8 cut(s) 242, 1402, 1642, 1763, 2666, 3042, 3214, 4006
BseJI GATNNNNATC 1 cut(s) 525
BseMII CTCAG 9 cut(s) 1206, 1226, 1329, 2427, 3450, 3868, 4035, 4085, 4202
BseNI ACTGG 9 cut(s) 72, 130, 1482, 2478, 3130, 3384, 3835, 4182, 4237
BseRI GAGGAG 2 cut(s) 1715, 4084
BseSI GKGCMC 2 cut(s) 254, 695
BseYI CCCAGC 3 cut(s) 754, 3081, 3467
Bsh1236I CGCG 2 cut(s) 1583, 3403
Bsh1285I CGRYCG 2 cut(s) 3427, 3948
BshTI ACCGGT 1 cut(s) 1622
BsiEI CGRYCG 2 cut(s) 3427, 3948
BsiHKAI GWGCWC 3 cut(s) 254, 695, 3944
BsiHKCI CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
BsiSI CCGG 6 cut(s) 1596, 1623, 1765, 1772, 3174, 4229
BsiWI CGTACG 1 cut(s) 1751
BslFI GGGAC 4 cut(s) 183, 883, 2911, 3481
BsmAI GTCTC 9 cut(s) 939, 1215, 1334, 2093, 2972, 3108, 3530, 3551, 3554
BsmBI CGTCTC 5 cut(s) 939, 1334, 3108, 3530, 3554
BsmFI GGGAC 4 cut(s) 183, 883, 2911, 3481
BsmI GAATGC 3 cut(s) 570, 2480, 3881
Bso31I GGTCTC 1 cut(s) 3551
BsoBI CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 3 cut(s) 254, 695, 3944
Bsp1407I TGTACA 3 cut(s) 2578, 2686, 4003
Bsp19I CCATGG 3 cut(s) 728, 2670, 3408
BspCNI CTCAG 9 cut(s) 1205, 1225, 1330, 2426, 3449, 3867, 4036, 4084, 4203
BspFNI CGCG 2 cut(s) 1583, 3403
BspLI GGNNCC 6 cut(s) 106, 118, 305, 3496, 3497, 3909
BspMAI CTGCAG 3 cut(s) 315, 1632, 3316
BspMI ACCTGC 3 cut(s) 1678, 1718, 3319
BspOI GCTAGC 1 cut(s) 1383
BspPI GGATC 6 cut(s) 111, 124, 512, 792, 2973, 3479
BspQI GCTCTTC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 417
BspTI CTTAAG 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 3551
BsrFI RCCGGY 2 cut(s) 1622, 3173
BsrGI TGTACA 3 cut(s) 2578, 2686, 4003
BsrI ACTGG 9 cut(s) 72, 130, 1482, 2478, 3130, 3384, 3835, 4182, 4237
BssAI RCCGGY 2 cut(s) 1622, 3173
BssECI CCNNGG 6 cut(s) 100, 728, 1111, 2670, 3408, 4228
BssNAI GTATAC 1 cut(s) 2749
BssNI GRCGYC 1 cut(s) 948
BssSI CACGAG 1 cut(s) 483
BssT1I CCWWGG 5 cut(s) 100, 728, 1111, 2670, 3408
Bst1107I GTATAC 1 cut(s) 2749
Bst2BI CACGAG 1 cut(s) 483
Bst2UI CCWGG 3 cut(s) 114, 1095, 1811
Bst6I CTCTTC 8 cut(s) 220, 1119, 1893, 2091, 2106, 2298, 2535, 2751
BstACI GRCGYC 1 cut(s) 948
BstAFI CTTAAG 1 cut(s) 362
BstAPI GCANNNNNTGC 1 cut(s) 1592
BstAUI TGTACA 3 cut(s) 2578, 2686, 4003
BstBAI YACGTR 2 cut(s) 581, 1320
BstBI TTCGAA 1 cut(s) 417
BstDSI CCRYGG 3 cut(s) 728, 2670, 3408
BstEII GGTNACC 1 cut(s) 641
BstENI CCTNNNNNAGG 2 cut(s) 311, 1652
BstF5I GGATG 8 cut(s) 242, 1402, 1642, 1763, 2666, 3042, 3214, 4006
BstFNI CGCG 2 cut(s) 1583, 3403
BstHHI GCGC 2 cut(s) 1583, 3405
BstMAI GTCTC 9 cut(s) 939, 1215, 1334, 2093, 2972, 3108, 3530, 3551, 3554
BstMCI CGRYCG 2 cut(s) 3427, 3948
BstNI CCWGG 3 cut(s) 114, 1095, 1811
BstNSI RCATGY 5 cut(s) 1887, 1951, 2292, 2356, 2776
BstPI GGTNACC 1 cut(s) 641
BstSCI CCNGG 5 cut(s) 112, 1093, 1809, 4227, 4228
BstSFI CTRYAG 3 cut(s) 311, 1628, 3312
BstSLI GKGCMC 2 cut(s) 254, 695
BstUI CGCG 2 cut(s) 1583, 3403
BstV2I GAAGAC 3 cut(s) 426, 642, 3630
BstX2I RGATCY 2 cut(s) 116, 784
BstXI CCANNNNNNTGG 2 cut(s) 3569, 4244
BstYI RGATCY 2 cut(s) 116, 784
BstZ17I GTATAC 1 cut(s) 2749
BsuI GTATCC 2 cut(s) 1303, 3655
BtgI CCRYGG 3 cut(s) 728, 2670, 3408
BtgZI GCGATG 1 cut(s) 874
BtsCI GGATG 8 cut(s) 242, 1402, 1642, 1763, 2666, 3042, 3214, 4006
BtsI GCAGTG 3 cut(s) 748, 1586, 3447
BveI ACCTGC 3 cut(s) 1678, 1718, 3319
CaiI CAGNNNCTG 2 cut(s) 323, 901
CfoI GCGC 2 cut(s) 1583, 3405
Cfr10I RCCGGY 2 cut(s) 1622, 3173
Cfr13I GGNCC 4 cut(s) 1620, 3398, 3495, 4179
Cfr9I CCCGGG 1 cut(s) 4228
CpoI CGGWCCG 1 cut(s) 1620
CseI GACGC 3 cut(s) 1816, 3536, 3547
CsiI ACCWGGT 1 cut(s) 1093
CspAI ACCGGT 1 cut(s) 1622
CspI CGGWCCG 1 cut(s) 1620
DraIII CACNNNGTG 1 cut(s) 1097
DrdI GACNNNNNNGTC 1 cut(s) 1623
DseDI GACNNNNNNGTC 1 cut(s) 1623
EaeI YGGCCR 3 cut(s) 1927, 2475, 3571
Eam1104I CTCTTC 8 cut(s) 220, 1119, 1893, 2091, 2106, 2298, 2535, 2751
EarI CTCTTC 8 cut(s) 220, 1119, 1893, 2091, 2106, 2298, 2535, 2751
EciI GGCGGA 2 cut(s) 109, 1772
Eco130I CCWWGG 5 cut(s) 100, 728, 1111, 2670, 3408
Eco147I AGGCCT 3 cut(s) 464, 1404, 3273
Eco31I GGTCTC 1 cut(s) 3551
Eco32I GATATC 3 cut(s) 1878, 2283, 2709
Eco47I GGWCC 2 cut(s) 1620, 3495
Eco57I CTGAAG 5 cut(s) 759, 1220, 3260, 3624, 4119
Eco88I CYCGRG 5 cut(s) 1792, 2276, 3886, 4060, 4228
Eco91I GGTNACC 1 cut(s) 641
EcoNI CCTNNNNNAGG 2 cut(s) 311, 1652
EcoO109I RGGNCCY 1 cut(s) 3495
EcoO65I GGTNACC 1 cut(s) 641
EcoRII CCWGG 3 cut(s) 112, 1093, 1809
EcoRV GATATC 3 cut(s) 1878, 2283, 2709
EcoT14I CCWWGG 5 cut(s) 100, 728, 1111, 2670, 3408
EcoT22I ATGCAT 1 cut(s) 3996
ErhI CCWWGG 5 cut(s) 100, 728, 1111, 2670, 3408
Esp3I CGTCTC 5 cut(s) 939, 1334, 3108, 3530, 3554
FalI AAGNNNNNCTT 6 cut(s) 665, 697, 2103, 2135, 3138, 3170
FaqI GGGAC 4 cut(s) 183, 883, 2911, 3481
FauI CCCGC 3 cut(s) 170, 1953, 3408
FauNDI CATATG 1 cut(s) 1332
FbaI TGATCA 2 cut(s) 2215, 3039
FblI GTMKAC 3 cut(s) 199, 2748, 3427
FokI GGATG 8 cut(s) 229, 1409, 1649, 1770, 2653, 3029, 3221, 4013
GlaI GCGC 2 cut(s) 1582, 3404
GsaI CCCAGC 3 cut(s) 758, 3085, 3471
GsuI CTGGAG 3 cut(s) 147, 3075, 3315
HapII CCGG 6 cut(s) 1596, 1623, 1765, 1772, 3174, 4229
HgaI GACGC 3 cut(s) 1816, 3536, 3547
HhaI GCGC 2 cut(s) 1583, 3405
Hin1I GRCGYC 1 cut(s) 948
Hin6I GCGC 2 cut(s) 1581, 3403
HinP1I GCGC 2 cut(s) 1581, 3403
HincII GTYRAC 4 cut(s) 2190, 2770, 3201, 3428
HindII GTYRAC 4 cut(s) 2190, 2770, 3201, 3428
HindIII AAGCTT 6 cut(s) 512, 1570, 2117, 2224, 2626, 3059
HpaI GTTAAC 2 cut(s) 2190, 2770
HpaII CCGG 6 cut(s) 1596, 1623, 1765, 1772, 3174, 4229
HphI GGTGA 8 cut(s) 151, 635, 804, 1083, 1181, 1987, 2392, 3119
Hpy99I CGWCG 3 cut(s) 953, 1580, 1832
HpyCH4IV ACGT 7 cut(s) 580, 948, 1319, 2656, 3117, 3515, 4159
HpySE526I ACGT 7 cut(s) 580, 948, 1319, 2656, 3117, 3515, 4159
Hsp92I GRCGYC 1 cut(s) 948
HspAI GCGC 2 cut(s) 1581, 3403
KflI GGGWCCC 1 cut(s) 3495
Ksp22I TGATCA 2 cut(s) 2215, 3039
KspAI GTTAAC 2 cut(s) 2190, 2770
LguI GCTCTTC 1 cut(s) 220
LmnI GCTCC 4 cut(s) 16, 2054, 2942, 3094
LweI GCATC 6 cut(s) 124, 329, 1507, 1748, 2864, 4003
MabI ACCWGGT 1 cut(s) 1093
MaeII ACGT 7 cut(s) 580, 948, 1319, 2656, 3117, 3515, 4159
MaeIII GTNAC 9 cut(s) 641, 971, 2140, 2921, 3047, 3118, 3673, 4011, 4084
MfeI CAATTG 3 cut(s) 1013, 2792, 3892
MflI RGATCY 2 cut(s) 116, 784
MhlI GDGCHC 3 cut(s) 254, 695, 3944
MlsI TGGCCA 1 cut(s) 3573
MluNI TGGCCA 1 cut(s) 3573
MlyI GAGTC 9 cut(s) 1033, 1650, 1703, 1912, 2095, 2543, 3089, 3155, 4313
MmeI TCCRAC 5 cut(s) 1035, 1614, 2021, 2430, 3009
Mox20I TGGCCA 1 cut(s) 3573
Mph1103I ATGCAT 1 cut(s) 3996
MroXI GAANNNNTTC 1 cut(s) 3368
MscI TGGCCA 1 cut(s) 3573
Msp20I TGGCCA 1 cut(s) 3573
MspA1I CMGCKG 3 cut(s) 1800, 1821, 3569
MspCI CTTAAG 1 cut(s) 362
MspI CCGG 6 cut(s) 1596, 1623, 1765, 1772, 3174, 4229
MspR9I CCNGG 5 cut(s) 114, 1095, 1811, 4229, 4230
MunI CAATTG 3 cut(s) 1013, 2792, 3892
Mva1269I GAATGC 3 cut(s) 570, 2480, 3881
MvaI CCWGG 3 cut(s) 114, 1095, 1811
MvnI CGCG 2 cut(s) 1583, 3403
NciI CCSGG 2 cut(s) 4229, 4230
NcoI CCATGG 3 cut(s) 728, 2670, 3408
NdeI CATATG 1 cut(s) 1332
NheI GCTAGC 1 cut(s) 1379
NlaIV GGNNCC 6 cut(s) 106, 118, 305, 3496, 3497, 3909
NmuCI GTSAC 4 cut(s) 641, 3047, 4011, 4084
NsiI ATGCAT 1 cut(s) 3996
NspI RCATGY 5 cut(s) 1887, 1951, 2292, 2356, 2776
NspV TTCGAA 1 cut(s) 417
PaeR7I CTCGAG 2 cut(s) 2276, 3886
PceI AGGCCT 3 cut(s) 464, 1404, 3273
PciI ACATGT 4 cut(s) 1883, 1947, 2288, 2352
PciSI GCTCTTC 1 cut(s) 220
PctI GAATGC 3 cut(s) 570, 2480, 3881
PdmI GAANNNNTTC 1 cut(s) 3368
Pfl23II CGTACG 1 cut(s) 1751
PflMI CCANNNNNTGG 1 cut(s) 1477
PfoI TCCNGGA 1 cut(s) 112
PinAI ACCGGT 1 cut(s) 1622
Ple19I CGATCG 1 cut(s) 3948
PleI GAGTC 9 cut(s) 1033, 1649, 1702, 1911, 2095, 2543, 3089, 3155, 4312
PpsI GAGTC 9 cut(s) 1033, 1649, 1702, 1911, 2095, 2543, 3089, 3155, 4312
Ppu21I YACGTR 2 cut(s) 581, 1320
PpuMI RGGWCCY 1 cut(s) 3495
PscI ACATGT 4 cut(s) 1883, 1947, 2288, 2352
PsiI TTATAA 3 cut(s) 1547, 1956, 2061
Psp1406I AACGTT 2 cut(s) 3515, 4159
Psp5II RGGWCCY 1 cut(s) 3495
Psp6I CCWGG 3 cut(s) 112, 1093, 1809
PspEI GGTNACC 1 cut(s) 641
PspFI CCCAGC 3 cut(s) 754, 3081, 3467
PspGI CCWGG 3 cut(s) 112, 1093, 1809
PspLI CGTACG 1 cut(s) 1751
PspN4I GGNNCC 6 cut(s) 106, 118, 305, 3496, 3497, 3909
PspPI GGNCC 4 cut(s) 1620, 3398, 3495, 4179
PspPPI RGGWCCY 1 cut(s) 3495
PspXI VCTCGAGB 1 cut(s) 3886
PstI CTGCAG 3 cut(s) 315, 1632, 3316
PstNI CAGNNNCTG 2 cut(s) 323, 901
PsuI RGATCY 2 cut(s) 116, 784
PvuI CGATCG 1 cut(s) 3948
PvuII CAGCTG 2 cut(s) 1800, 3569
Rsr2I CGGWCCG 1 cut(s) 1620
RsrII CGGWCCG 1 cut(s) 1620
SalI GTCGAC 1 cut(s) 3426
SapI GCTCTTC 1 cut(s) 220
Sau96I GGNCC 4 cut(s) 1620, 3398, 3495, 4179
SbfI CCTGCAGG 1 cut(s) 315
ScaI AGTACT 1 cut(s) 4277
SchI GAGTC 9 cut(s) 1033, 1650, 1703, 1912, 2095, 2543, 3089, 3155, 4313
ScrFI CCNGG 5 cut(s) 114, 1095, 1811, 4229, 4230
SdaI CCTGCAGG 1 cut(s) 315
SduI GDGCHC 3 cut(s) 254, 695, 3944
SexAI ACCWGGT 1 cut(s) 1093
SfaNI GCATC 6 cut(s) 124, 329, 1507, 1748, 2864, 4003
SfcI CTRYAG 3 cut(s) 311, 1628, 3312
Sfr274I CTCGAG 2 cut(s) 2276, 3886
SfuI TTCGAA 1 cut(s) 417
SinI GGWCC 2 cut(s) 1620, 3495
SlaI CTCGAG 2 cut(s) 2276, 3886
SmaI CCCGGG 1 cut(s) 4230
SmlI CTYRAG 6 cut(s) 362, 1485, 1871, 2276, 3292, 3886
SmoI CTYRAG 6 cut(s) 362, 1485, 1871, 2276, 3292, 3886
Sse8387I CCTGCAGG 1 cut(s) 315
SseBI AGGCCT 3 cut(s) 464, 1404, 3273
SspI AATATT 2 cut(s) 2497, 3698
StuI AGGCCT 3 cut(s) 464, 1404, 3273
StyD4I CCNGG 5 cut(s) 112, 1093, 1809, 4227, 4228
StyI CCWWGG 5 cut(s) 100, 728, 1111, 2670, 3408
TaiI ACGT 7 cut(s) 583, 951, 1322, 2659, 3120, 3518, 4162
TaqII GACCGA 1 cut(s) 2756
TatI WGTACW 5 cut(s) 2203, 2578, 2686, 4003, 4275
TauI GCSGC 4 cut(s) 1586, 1809, 1821, 2480
TseFI GTSAC 4 cut(s) 641, 3047, 4011, 4084
Tsp45I GTSAC 4 cut(s) 641, 3047, 4011, 4084
TspGWI ACGGA 8 cut(s) 517, 876, 1615, 1633, 1768, 1826, 2424, 3035
TspMI CCCGGG 1 cut(s) 4228
Van91I CCANNNNNTGG 1 cut(s) 1477
Vha464I CTTAAG 1 cut(s) 362
VneI GTGCAC 2 cut(s) 250, 691
VpaK11BI GGWCC 2 cut(s) 1620, 3495
XagI CCTNNNNNAGG 2 cut(s) 311, 1652
XapI RAATTY 5 cut(s) 236, 1409, 2849, 3263, 3594
XbaI TCTAGA 2 cut(s) 787, 3598
XceI RCATGY 5 cut(s) 1887, 1951, 2292, 2356, 2776
XcmI CCANNNNNNNNNTGG 2 cut(s) 3994, 4244
XhoI CTCGAG 2 cut(s) 2276, 3886
XmaI CCCGGG 1 cut(s) 4228
XmaJI CCTAGG 1 cut(s) 1111
XmiI GTMKAC 3 cut(s) 199, 2748, 3427
XmnI GAANNNNTTC 1 cut(s) 3368
ZraI GACGTC 1 cut(s) 949
ZrmI AGTACT 1 cut(s) 4277
Zsp2I ATGCAT 1 cut(s) 3996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.