RLG00000003593

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003701
Physical Location & Seq
Forward (+)
50442182 .. 50447703
5522 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003593

Sequence Viewer

Length: 3831 bp
ATGGCGATTAGATTTACGGAGGCAATTCTTTTTAAAACTAAGGAGGCAATTGAACTCGTAGGACGCCAAGTGGGATATGTAATTCACTGCAAAAGCTACCTTCAAAGACTGCAGGCTCAATCGGAAGAATTGAATGCCGCCCGGGAGGCGGTAGAGGATAAAGTTGTGGAAGCTGAAGACAAAGGTGAAGCAGTTCAACCAGATGTCCAGTTGTGGCTACAGGAAGTGGGCAAAATCAATGCAGAGGCAGAGGAATTGTTGAAAGATGAAAACCAAGCCAAGCTGAAATGTCTCCATGGGTTTTGTCCTAATCTGACAATCCGTCATCACCTAGGCAGGAAATCAAGAAAATTGGTGTACCATCTTGTTGAGCTCTATGAAAGACGAGAATTTCCCGTTATTTCTGTTATTCAGCCGCAAGAGCTTTGGGTTGAGTCCACCGGAGAGTACCTGGCCTTTCCGTCAAGGACTTCAATTGTGGCGCAAATCATGGATGAACTTGAGAATCCTAATACTCATAGGATCGGGGTGTATGGAATTGAAGGTGTGGGTAAAACCACACTAGTAAAAGAAGTCTATAGGCAAGCTACAGAAGATAGAAAGTTATTTGACGATGTGGTTATCCTACTAGATGTGAAAGAAAATCCACACTTGGAAGGAATTCAAAACAAAATTTTAAGTAGGTTAGATATGAAGATTTTTGACAATGAGAATTTAGATGGAAGAGCAAGTCGTACATCTGCCGATATACAGGACAAGAACATCCTTGTAATTCTGGATGATGTTTGGGAACAAATTGACTTGGAGGCTCTCCAACTTCCAAGTGTGGCTACTTGTAAGATATTGCTGACATATACAAGTAGAACATCATTATCTGGTATGAGCACGCAGCAAGATTTTCAGCTTGACATCTTGGAAGAAGAAGAGGCTTGGATTCTATTTAAGAAGAAGGCAGGCAATGTTGTTAAGAATTATGCTATACGAGAAGTAGCACTCCAAGTAGCCGAATGTTGTAGTGGCTTGCCTATTTTTGTTGTCACAGTTGCAAGCGCTTTGAAGAAACAAACTACTTCACCTCCATGGAGAGATGCATTGAGATGCCTAAAAAGCCAAGAATTGACAGAGAACCCATACTTGTGTCTGGAGTGGAGTTACGATCAGCTGAAGGATAAGGAACTTGAACAGTTGTTCTTGCTATGTGGATTTGCTATATGGGGAAAGGATATTTATTTGCCAGCCTTGCTGAAATATAGTATGGGTTTGGGTCTCTTTAGAAAGGGATACACAGTGGAGGAAGCACAAAATACATTGCTTAAATGGGTTGGAAAACTTAAAGATTCTTGTCTACTGACAGACAGTGATGATAGCAGATATGTCAAAATGCATAACCTTGTGCGTGTTGTTGCTAACCGGATTGCACTCAGAGATGAACACATCTTATCATCAGTCGATGATAGGGGAAATTATGAATTTAAGGAATGGCTAGATAAGGATTTCTTCAAAAAGTGCACTATGAAATCTTTACAATCTAGTACTGTTCCTGTGCTTTCTGAAGTACCTAGGATATATCCCAAATTAAAGCTGTTCTATTTACACGGTACAGGTGAAGCGTCAGTGGAAATCCCAACAAACTTTTTTAGAGAGATTGAGGAACTAAAAGTGTTGGATTTAACAAGACTGCATATGCTGTCACTACCTTCCTCTCTTCAGTACTTGAAGAATCTGCATGCCCTATTTTTAGATCAATGCATAGTGGGAGACACTGCTTTAGTTGGGGAGCTAAGTAACTTGGAAATTCTTAGCTTTTTCCAATCCAATGTCAAACAATTACCAAAGGAAATAGGGAAACTGACCCGTCTTCAGTTGCTGGATTTATCTGATTGCTCTGAACTCGAAGTTATTTCACCTGGTGTCATATCAAGCTTGGAGGGACTACAAGAATTGAGAATGCGAAACAGCTTCAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTAGTGAGTTGAAGCATTTGTCTCAGCTATCTGTATTAGAGATACATATTCCGAATACTGACATCCTTCCAGCAAACTTGTTCTCCCATGAGTTCGAAAGATTCAATATTGTTATTGGGGATGCATGGGTTGACACATATACATCACAAGAGAAATTCATGACTGAGACGACCCTCAAGACACTAAAACTCAAACTCACTACCAGCATTAAAGAATTGGATGAAGGATTAATATGGCTGCTGAAGAGATGTGAAGACTTGTCCTTAAGTGAGACACAGAATGCTAATAATATTGACCATTATTTAGATACTGAAGATTCTGAGCATCTGCAAGTCATAAACATCTGTAAGATTGATTTGCCCAACTTAACAAGATTGGCTGTTCACCGATGTGATGGTTTAAGTTTCTTATTGTCATCTTCCGTGGCAAAAAGTTTTGTACAACTTAAACATCTTCGGATATCTGAATGTCAAATCATGAAAGAGGTAGTATTGTCAGAAGGTCACGGTGAAGAAAACAAGGATAACTTGTTTCCGAAGTTAGAACAATTGGAGCTAAATGATCTTCCTTGTCTCACTAGATTCTGCTCAGGAAGTTATGTTGAGTTTGCAGCTCTGAAGAGGTCGCAATTGGAGAAGTGTCCTAAATTGGAGACATTCATCTTTAATCATTTGAGTAACAGAATTCAAATCTACAAAGAAATTAAAGAAAGGGGCACAGATGATGAGAATGTTGAGACATTCGCACCATACTTTCTTTTCGACGAAAATGTGAAAACGTTGAGGCTGCATGGAATGGAGATCAAGCTCATGCGTCTCTCAGAAAAGAGCCCAGTTTTTCCACTGTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAACCTTAAGTTGTCTGCAATATCCTTTCGGAATCTAACAATTATAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCAAAGTTTAGTGGGACTGAAAAAATTGAAAGTTGATGAATGTAAAAATATGAAAGAAATCTTCACAAGTGAAGGAATTGAAGAAGATGCAAGTAATTGTGAGATTGTTCTCAGCCGGTTGCAACATTTGGAACTCAGTGATTTAATAAGTTTGGAACGGTTTTGCTCGAGAAATTGCATTGTGAAAGTCCCATCCTTGCAAAGTAGTGCTTTACAGGTAAATTGGTGCCCATTCCAGTTGAAGATTTCCACTGAAAGGGTACTAGAAGAAGTTTCCGATATGCCACAGGATGTACATAAGTCGCATACTGGTAGAGAAGAGGTTCTCGACACAACTGAGTCTGCTTGTAGCCTGGAAACAAATCAGAAACAGCACGTCCCAAACACCTCTGAAGTTTCTAAAGAAATCGAGTTTAATGCAGGAGAACTCCATGATGACAGACTTGTAAACAACGACAGGCCTCTATTGCCTGGCAATATTGCTGAAAGCAGTGAACATGGAGTCAAAGCTGTCATTAAAGAAACTCCTATCAATTTCCTGGAGCATGCTGAGGTTATGGATCTACGTCAGAAGCAAATTGTCACCAATACGCCAAAGAATCTTCAGGAGGCTGATTTTGGCATGGCAGAGAGCCTCATTGAACCTTTGAATGCAAATGAAGGCAGACTACTTCAATTGAAACAACTTGATGGGGTGCAAACTGATTCATTGCTGCAAAGCATATCTGAGCTTGATGCAGACATAAAACATTTGCAGGAAGAATTAAGGGCCAAAGAAGCAGAGAGGAGAGATAAACAGCAAAGGCTTGGCGACAGACTTGCGACAGTTGATCGGTTGTCCGATTCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1277

Amino Acids

145.81

Weight (kDa)

5.21

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 159 - 319 8.4e-24 NB-ARC domain
LRR_14 PF23598 591 - 854 2.8e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 788 - 887 3.3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 961 - 1069 7.7e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 3204
AccI GTMKAC 1 cut(s) 1345
AciI CCGC 3 cut(s) 138, 149, 416
AclI AACGTT 1 cut(s) 2780
AclWI GGATC 2 cut(s) 530, 3546
AcsI RAATTY 9 cut(s) 389, 660, 672, 712, 1469, 1794, 2156, 2685, 2853
AcyI GRCGYC 1 cut(s) 64
AdeI CACNNNGTG 1 cut(s) 1910
AfeI AGCGCT 1 cut(s) 1051
AfiI CCNNNNNNNGG 2 cut(s) 148, 3234
AflII CTTAAG 2 cut(s) 2266, 2888
AhdI GACNNNNNGTC 1 cut(s) 321
AhlI ACTAGT 1 cut(s) 562
AjiI CACGTC 1 cut(s) 3355
AjnI CCWGG 5 cut(s) 450, 1906, 3330, 3448, 3516
AleI CACNNNNGTG 1 cut(s) 2391
Alw21I GWGCWC 3 cut(s) 375, 887, 1511
Alw44I GTGCAC 1 cut(s) 1507
AlwI GGATC 2 cut(s) 530, 3546
AlwNI CAGNNNCTG 1 cut(s) 1867
Ama87I CYCGRG 2 cut(s) 141, 3145
Aor51HI AGCGCT 1 cut(s) 1051
AoxI GGCC 3 cut(s) 453, 3437, 3747
ApaLI GTGCAC 1 cut(s) 1507
ApeKI GCWGC 5 cut(s) 889, 2239, 2612, 2788, 3691
ApoI RAATTY 9 cut(s) 389, 660, 672, 712, 1469, 1794, 2156, 2685, 2853
ArsI GACNNNNNNTTYG 2 cut(s) 2953, 2985
AseI ATTAAT 1 cut(s) 2231
Asp700I GAANNNNTTC 3 cut(s) 192, 660, 3300
AspA2I CCTAGG 2 cut(s) 331, 1559
AspLEI GCGC 2 cut(s) 484, 1052
AspS9I GGNCC 1 cut(s) 3747
AsuC2I CCSGG 2 cut(s) 142, 143
AsuII TTCGAA 1 cut(s) 2097
AvaI CYCGRG 2 cut(s) 141, 3145
AvrII CCTAGG 2 cut(s) 331, 1559
BaeGI GKGCMC 3 cut(s) 1511, 2720, 3209
BanI GGYRCC 1 cut(s) 3204
BanII GRGCYC 2 cut(s) 375, 2834
BarI GAAGNNNNNNTAC 2 cut(s) 3630, 3662
BbsI GAAGAC 3 cut(s) 183, 1850, 2262
Bbv12I GWGCWC 3 cut(s) 375, 887, 1511
BbvCI CCTCAGC 1 cut(s) 3528
BbvI GCAGC 5 cut(s) 901, 2226, 2624, 2775, 3678
BccI CCATC 5 cut(s) 369, 713, 2390, 3178, 3662
BcgI CGANNNNNNTGC 4 cut(s) 3377, 3411, 3779, 3813
BciT130I CCWGG 5 cut(s) 452, 1908, 3332, 3450, 3518
BciVI GTATCC 1 cut(s) 1274
BcnI CCSGG 2 cut(s) 142, 143
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2580, 2858, 3242
BfmI CTRYAG 4 cut(s) 110, 218, 577, 588
BfoI RGCGCY 1 cut(s) 1053
BfrI CTTAAG 2 cut(s) 2266, 2888
BfuI GTATCC 1 cut(s) 1274
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 7 cut(s) 138, 416, 890, 2240, 2613, 2789, 3692
BlnI CCTAGG 2 cut(s) 331, 1559
BlsI GCNGC 7 cut(s) 139, 417, 891, 2241, 2614, 2790, 3693
BmcAI AGTACT 2 cut(s) 1534, 1712
Bme1390I CCNGG 7 cut(s) 142, 143, 452, 1908, 3332, 3450, 3518
BmeRI GACNNNNNGTC 1 cut(s) 321
BmeT110I CYCGRG 2 cut(s) 141, 3145
BmgBI CACGTC 1 cut(s) 3355
BmgT120I GGNCC 1 cut(s) 3747
BmiI GGNNCC 1 cut(s) 3206
BmrFI CCNGG 7 cut(s) 142, 143, 452, 1908, 3332, 3450, 3518
BmrI ACTGGG 1 cut(s) 2828
BmsI GCATC 6 cut(s) 1078, 1088, 2113, 2335, 3055, 3703
BmuI ACTGGG 1 cut(s) 2828
BpiI GAAGAC 3 cut(s) 183, 1850, 2262
BpmI CTGGAG 2 cut(s) 1163, 3539
Bpu10I CCTNAGC 2 cut(s) 2590, 3528
Bpu14I TTCGAA 1 cut(s) 2097
BpuEI CTTGAG 2 cut(s) 521, 2162
BpuMI CCSGG 2 cut(s) 142, 143
BsaHI GRCGYC 1 cut(s) 64
BsaI GGTCTC 1 cut(s) 1271
BsaJI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2424
BsaWI WCCGGW 2 cut(s) 440, 1410
BsaXI ACNNNNNCTCC 4 cut(s) 1060, 1090, 2069, 2099
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 3234
Bse118I RCCGGY 1 cut(s) 3093
Bse1I ACTGG 4 cut(s) 208, 2834, 3214, 3292
Bse3DI GCAATG 3 cut(s) 964, 1307, 3686
BseBI CCWGG 5 cut(s) 452, 1908, 3332, 3450, 3518
BseDI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2424
BseGI GGATG 8 cut(s) 499, 762, 784, 2064, 2128, 2227, 3170, 3274
BseLI CCNNNNNNNGG 2 cut(s) 148, 3234
BseMI GCAATG 3 cut(s) 964, 1307, 3686
BseNI ACTGG 4 cut(s) 208, 2834, 3214, 3292
BseRI GAGGAG 1 cut(s) 3778
BseSI GKGCMC 3 cut(s) 1511, 2720, 3209
BseXI GCAGC 5 cut(s) 901, 2226, 2624, 2775, 3678
BshFI GGCC 3 cut(s) 455, 3439, 3749
BshNI GGYRCC 1 cut(s) 3204
BsiHKAI GWGCWC 3 cut(s) 375, 887, 1511
BsiHKCI CYCGRG 2 cut(s) 141, 3145
BsiSI CCGG 4 cut(s) 142, 441, 1411, 3094
BslFI GGGAC 4 cut(s) 1944, 3006, 3152, 3341
BslI CCNNNNNNNGG 2 cut(s) 148, 3234
BsmBI CGTCTC 2 cut(s) 2162, 2822
BsmFI GGGAC 4 cut(s) 1944, 3006, 3152, 3341
BsmI GAATGC 4 cut(s) 139, 1953, 2287, 3634
BsnI GGCC 3 cut(s) 455, 3439, 3749
Bso31I GGTCTC 1 cut(s) 1271
BsoBI CYCGRG 2 cut(s) 141, 3145
Bsp119I TTCGAA 1 cut(s) 2097
Bsp1286I GDGCHC 6 cut(s) 375, 887, 1511, 2720, 2834, 3209
Bsp1407I TGTACA 2 cut(s) 2440, 3271
Bsp143I GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
Bsp19I CCATGG 2 cut(s) 295, 1079
BspACI CCGC 3 cut(s) 138, 149, 416
BspANI GGCC 3 cut(s) 455, 3439, 3749
BspHI TCATGA 2 cut(s) 2160, 2478
BspLI GGNNCC 1 cut(s) 3206
BspMAI CTGCAG 1 cut(s) 114
BspPI GGATC 2 cut(s) 530, 3546
BspQI GCTCTTC 1 cut(s) 718
BspT104I TTCGAA 1 cut(s) 2097
BspT107I GGYRCC 1 cut(s) 3204
BspTI CTTAAG 2 cut(s) 2266, 2888
BspTNI GGTCTC 1 cut(s) 1271
BsrDI GCAATG 3 cut(s) 964, 1307, 3686
BsrFI RCCGGY 1 cut(s) 3093
BsrGI TGTACA 2 cut(s) 2440, 3271
BsrI ACTGG 4 cut(s) 208, 2834, 3214, 3292
BssAI RCCGGY 1 cut(s) 3093
BssECI CCNNGG 6 cut(s) 141, 295, 331, 1079, 1559, 2424
BssMI GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
BssNI GRCGYC 1 cut(s) 64
BssT1I CCWWGG 4 cut(s) 295, 331, 1079, 1559
Bst2UI CCWGG 5 cut(s) 452, 1908, 3332, 3450, 3518
Bst6I CTCTTC 6 cut(s) 718, 918, 1710, 2240, 2615, 3291
BstACI GRCGYC 1 cut(s) 64
BstAFI CTTAAG 2 cut(s) 2266, 2888
BstAUI TGTACA 2 cut(s) 2440, 3271
BstBI TTCGAA 1 cut(s) 2097
BstDSI CCRYGG 3 cut(s) 295, 1079, 2424
BstF5I GGATG 8 cut(s) 499, 762, 784, 2064, 2128, 2227, 3170, 3274
BstH2I RGCGCY 1 cut(s) 1053
BstHHI GCGC 2 cut(s) 484, 1052
BstKTI GATC 7 cut(s) 525, 1159, 1744, 2566, 2805, 3541, 3811
BstMBI GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
BstMWI GCNNNNNNNGC 6 cut(s) 146, 421, 1107, 1240, 3445, 3755
BstNI CCWGG 5 cut(s) 452, 1908, 3332, 3450, 3518
BstNSI RCATGY 2 cut(s) 1730, 3527
BstSCI CCNGG 7 cut(s) 140, 141, 450, 1906, 3330, 3448, 3516
BstSFI CTRYAG 4 cut(s) 110, 218, 577, 588
BstSLI GKGCMC 3 cut(s) 1511, 2720, 3209
BstV1I GCAGC 5 cut(s) 901, 2226, 2624, 2775, 3678
BstV2I GAAGAC 3 cut(s) 183, 1850, 2262
BstX2I RGATCY 1 cut(s) 3538
BstYI RGATCY 1 cut(s) 3538
BsuI GTATCC 1 cut(s) 1274
BsuRI GGCC 3 cut(s) 455, 3439, 3749
BtgI CCRYGG 3 cut(s) 295, 1079, 2424
BtrI CACGTC 1 cut(s) 3355
BtsCI GGATG 8 cut(s) 499, 762, 784, 2064, 2128, 2227, 3170, 3274
BtsI GCAGTG 3 cut(s) 85, 1761, 3475
BtsIMutI CAGTG 9 cut(s) 85, 1293, 1363, 1620, 1761, 2843, 3121, 3228, 3475
CaiI CAGNNNCTG 1 cut(s) 1867
CciI TCATGA 2 cut(s) 2160, 2478
CfoI GCGC 2 cut(s) 484, 1052
Cfr10I RCCGGY 1 cut(s) 3093
Cfr13I GGNCC 1 cut(s) 3747
Cfr9I CCCGGG 1 cut(s) 141
CseI GACGC 3 cut(s) 72, 1599, 2804
CsiI ACCWGGT 1 cut(s) 1906
DpnI GATC 7 cut(s) 524, 1158, 1743, 2565, 2804, 3540, 3810
DpnII GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
DraI TTTAAA 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 1910
DriI GACNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 6 cut(s) 718, 918, 1710, 2240, 2615, 3291
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 6 cut(s) 718, 918, 1710, 2240, 2615, 3291
Ecl136II GAGCTC 1 cut(s) 373
Eco130I CCWWGG 4 cut(s) 295, 331, 1079, 1559
Eco147I AGGCCT 1 cut(s) 3439
Eco24I GRGCYC 2 cut(s) 375, 2834
Eco31I GGTCTC 1 cut(s) 1271
Eco32I GATATC 1 cut(s) 2463
Eco47III AGCGCT 1 cut(s) 1051
Eco53kI GAGCTC 1 cut(s) 373
Eco88I CYCGRG 2 cut(s) 141, 3145
EcoICRI GAGCTC 1 cut(s) 373
EcoRI GAATTC 2 cut(s) 660, 2685
EcoRII CCWGG 5 cut(s) 450, 1906, 3330, 3448, 3516
EcoRV GATATC 1 cut(s) 2463
EcoT14I CCWWGG 4 cut(s) 295, 331, 1079, 1559
EcoT22I ATGCAT 4 cut(s) 1093, 1386, 1751, 2128
EcoT38I GRGCYC 2 cut(s) 375, 2834
ErhI CCWWGG 4 cut(s) 295, 331, 1079, 1559
Esp3I CGTCTC 2 cut(s) 2162, 2822
FalI AAGNNNNNCTT 6 cut(s) 1481, 1513, 2513, 2545, 3172, 3204
FaqI GGGAC 4 cut(s) 1944, 3006, 3152, 3341
FauNDI CATATG 1 cut(s) 1683
FblI GTMKAC 1 cut(s) 1345
Fnu4HI GCNGC 7 cut(s) 138, 416, 890, 2240, 2613, 2789, 3692
FokI GGATG 8 cut(s) 506, 749, 791, 2051, 2135, 2234, 3157, 3281
FriOI GRGCYC 2 cut(s) 375, 2834
Fsp4HI GCNGC 7 cut(s) 138, 416, 890, 2240, 2613, 2789, 3692
FspBI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2580, 2858, 3242
GlaI GCGC 2 cut(s) 483, 1051
GluI GCNGC 7 cut(s) 138, 416, 890, 2240, 2613, 2789, 3692
GsuI CTGGAG 2 cut(s) 1163, 3539
HaeII RGCGCY 1 cut(s) 1053
HaeIII GGCC 3 cut(s) 455, 3439, 3749
HapII CCGG 4 cut(s) 142, 441, 1411, 3094
HgaI GACGC 3 cut(s) 72, 1599, 2804
HhaI GCGC 2 cut(s) 484, 1052
Hin1I GRCGYC 1 cut(s) 64
Hin6I GCGC 2 cut(s) 482, 1050
HinP1I GCGC 2 cut(s) 482, 1050
HincII GTYRAC 1 cut(s) 2134
HindII GTYRAC 1 cut(s) 2134
HindIII AAGCTT 1 cut(s) 1921
HpaII CCGG 4 cut(s) 142, 441, 1411, 3094
Hpy166II GTNNAC 8 cut(s) 358, 438, 1346, 1509, 2134, 2386, 3427, 3473
Hpy8I GTNNAC 8 cut(s) 358, 438, 1346, 1509, 2134, 2386, 3427, 3473
Hpy99I CGWCG 1 cut(s) 2768
HpyCH4IV ACGT 3 cut(s) 2780, 3354, 3544
HpyF10VI GCNNNNNNNGC 6 cut(s) 146, 421, 1107, 1240, 3445, 3755
HpySE526I ACGT 3 cut(s) 2780, 3354, 3544
Hsp92I GRCGYC 1 cut(s) 64
HspAI GCGC 2 cut(s) 482, 1050
Kzo9I GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 3 cut(s) 1777, 2554, 3520
Lsp1109I GCAGC 5 cut(s) 901, 2226, 2624, 2775, 3678
LweI GCATC 6 cut(s) 1078, 1088, 2113, 2335, 3055, 3703
MabI ACCWGGT 1 cut(s) 1906
MaeI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2580, 2858, 3242
MaeII ACGT 3 cut(s) 2780, 3354, 3544
MaeIII GTNAC 8 cut(s) 1036, 1151, 1689, 1784, 2504, 2678, 2932, 3559
MalI GATC 7 cut(s) 524, 1158, 1743, 2565, 2804, 3540, 3810
MboI GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
MfeI CAATTG 5 cut(s) 48, 474, 2549, 2630, 3653
MflI RGATCY 1 cut(s) 3538
MhlI GDGCHC 6 cut(s) 375, 887, 1511, 2720, 2834, 3209
MlyI GAGTC 3 cut(s) 443, 3326, 3489
MmeI TCCRAC 4 cut(s) 838, 1303, 1644, 2829
Mph1103I ATGCAT 4 cut(s) 1093, 1386, 1751, 2128
MroXI GAANNNNTTC 3 cut(s) 192, 660, 3300
MslI CAYNNNNRTG 4 cut(s) 1078, 1096, 1135, 2391
MspA1I CMGCKG 1 cut(s) 1162
MspCI CTTAAG 2 cut(s) 2266, 2888
MspI CCGG 4 cut(s) 142, 441, 1411, 3094
MspR9I CCNGG 7 cut(s) 142, 143, 452, 1908, 3332, 3450, 3518
MunI CAATTG 5 cut(s) 48, 474, 2549, 2630, 3653
Mva1269I GAATGC 4 cut(s) 139, 1953, 2287, 3634
MvaI CCWGG 5 cut(s) 452, 1908, 3332, 3450, 3518
MwoI GCNNNNNNNGC 6 cut(s) 146, 421, 1107, 1240, 3445, 3755
NciI CCSGG 2 cut(s) 142, 143
NcoI CCATGG 2 cut(s) 295, 1079
NdeI CATATG 1 cut(s) 1683
NdeII GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
NlaIV GGNNCC 1 cut(s) 3206
NmuCI GTSAC 4 cut(s) 1036, 1689, 2504, 3559
NsiI ATGCAT 4 cut(s) 1093, 1386, 1751, 2128
NspI RCATGY 2 cut(s) 1730, 3527
NspV TTCGAA 1 cut(s) 2097
OliI CACNNNNGTG 1 cut(s) 2391
PaeI GCATGC 2 cut(s) 1730, 3527
PaeR7I CTCGAG 1 cut(s) 3145
PagI TCATGA 2 cut(s) 2160, 2478
PceI AGGCCT 1 cut(s) 3439
PciSI GCTCTTC 1 cut(s) 718
PctI GAATGC 4 cut(s) 139, 1953, 2287, 3634
PdmI GAANNNNTTC 3 cut(s) 192, 660, 3300
PfoI TCCNGGA 1 cut(s) 3516
PkrI GCNGC 7 cut(s) 139, 417, 891, 2241, 2614, 2790, 3693
PleI GAGTC 3 cut(s) 442, 3325, 3488
PpsI GAGTC 3 cut(s) 442, 3325, 3488
PshBI ATTAAT 1 cut(s) 2231
Psp124BI GAGCTC 1 cut(s) 375
Psp1406I AACGTT 1 cut(s) 2780
Psp6I CCWGG 5 cut(s) 450, 1906, 3330, 3448, 3516
PspGI CCWGG 5 cut(s) 450, 1906, 3330, 3448, 3516
PspN4I GGNNCC 1 cut(s) 3206
PspPI GGNCC 1 cut(s) 3747
PstI CTGCAG 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 1867
PsuI RGATCY 1 cut(s) 3538
PvuII CAGCTG 1 cut(s) 1162
RseI CAYNNNNRTG 4 cut(s) 1078, 1096, 1135, 2391
SacI GAGCTC 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 718
SatI GCNGC 7 cut(s) 138, 416, 890, 2240, 2613, 2789, 3692
Sau3AI GATC 7 cut(s) 522, 1156, 1741, 2563, 2802, 3538, 3808
Sau96I GGNCC 1 cut(s) 3747
ScaI AGTACT 2 cut(s) 1534, 1712
SchI GAGTC 3 cut(s) 443, 3326, 3489
ScrFI CCNGG 7 cut(s) 142, 143, 452, 1908, 3332, 3450, 3518
SduI GDGCHC 6 cut(s) 375, 887, 1511, 2720, 2834, 3209
SexAI ACCWGGT 1 cut(s) 1906
SfaNI GCATC 6 cut(s) 1078, 1088, 2113, 2335, 3055, 3703
SfcI CTRYAG 4 cut(s) 110, 218, 577, 588
Sfr274I CTCGAG 1 cut(s) 3145
SfuI TTCGAA 1 cut(s) 2097
SlaI CTCGAG 1 cut(s) 3145
SmaI CCCGGG 1 cut(s) 143
SmiMI CAYNNNNRTG 4 cut(s) 1078, 1096, 1135, 2391
SmlI CTYRAG 5 cut(s) 500, 2177, 2266, 2888, 3145
SmoI CTYRAG 5 cut(s) 500, 2177, 2266, 2888, 3145
SpeI ACTAGT 1 cut(s) 562
SphI GCATGC 2 cut(s) 1730, 3527
SseBI AGGCCT 1 cut(s) 3439
SsiI CCGC 3 cut(s) 138, 149, 416
SspI AATATT 4 cut(s) 2110, 2293, 2957, 3457
SspMI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2580, 2858, 3242
SstI GAGCTC 1 cut(s) 375
StuI AGGCCT 1 cut(s) 3439
StyD4I CCNGG 7 cut(s) 140, 141, 450, 1906, 3330, 3448, 3516
StyI CCWWGG 4 cut(s) 295, 331, 1079, 1559
TaiI ACGT 3 cut(s) 2783, 3357, 3547
TaqI TCGA 7 cut(s) 1449, 1893, 2097, 2763, 3146, 3306, 3387
TatI WGTACW 4 cut(s) 1532, 1710, 2440, 3271
TauI GCSGC 2 cut(s) 140, 418
TscAI CASTG 9 cut(s) 92, 1293, 1363, 1620, 1768, 2850, 3121, 3235, 3475
TseFI GTSAC 4 cut(s) 1036, 1689, 2504, 3559
TseI GCWGC 5 cut(s) 889, 2239, 2612, 2788, 3691
Tsp45I GTSAC 4 cut(s) 1036, 1689, 2504, 3559
TspGWI ACGGA 4 cut(s) 32, 311, 450, 2413
TspMI CCCGGG 1 cut(s) 141
TspRI CASTG 9 cut(s) 92, 1293, 1363, 1620, 1768, 2850, 3121, 3235, 3475
Vha464I CTTAAG 2 cut(s) 2266, 2888
VneI GTGCAC 1 cut(s) 1507
VspI ATTAAT 1 cut(s) 2231
XapI RAATTY 9 cut(s) 389, 660, 672, 712, 1469, 1794, 2156, 2685, 2853
XbaI TCTAGA 1 cut(s) 2857
XceI RCATGY 2 cut(s) 1730, 3527
XhoI CTCGAG 1 cut(s) 3145
XmaI CCCGGG 1 cut(s) 141
XmaJI CCTAGG 2 cut(s) 331, 1559
XmiI GTMKAC 1 cut(s) 1345
XmnI GAANNNNTTC 3 cut(s) 192, 660, 3300
XspI CTAG 9 cut(s) 332, 563, 629, 1484, 1530, 1560, 2580, 2858, 3242
ZrmI AGTACT 2 cut(s) 1534, 1712
Zsp2I ATGCAT 4 cut(s) 1093, 1386, 1751, 2128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.