RchiOBHm_Chr2g0111441

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003701
Physical Location & Seq
Reverse (-)
23095981 .. 23097944
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48503

Sequence Viewer

Length: 1860 bp
ATGGAAGCCATGGATTCAATTCTTTCAAAAATTGCCGAGTACACAGTTGAACCCGTTGGACGCCAAGTGGGTTATGTAATTCACTGCAACAGAAACCTTCACCACCTACAATATGGAGTGAGATGGTTAAAAGCTGAGAGAGACTATTTAATAACTGAGGTTGATGCGGCTGAAATAATAAATGAACGAGCTCAACCTGCTTTACAGGATTGGCTGGCAAGAGCGGAGGCGATCACTGTAATGGCAGAGGACCTTCTGAGAGATGGAAATCGAGCAAATCTGGAATGTCTCCATGGGTTTTGTCCTAATCTGAAGTACCGTTATCGGCTAGGCAGGAGATCAACAGGATTGCTGGATGAGATTGAGATGTTCCTGCACATAAAAGAAGCACTAATCTTGCAAATGGAGTGGGGTGTATCCACCGGGGAGTACCAGGCTTTTCGATCAAGGACGTCAACTGTGAGGCAAATCATGGATGAACTGAAGAAGTCTAGTGCCCGCAGGATTGGGGTGTGTGGACCTAGAGGTGTGGGTAAAACTCTACTTGGCAAAGAAGTTTATAGGCAAGCTAGAAAAGATAAAAAGTTATTTGATGATGTGGTTATCCTATTAGATGTCAAGAAAAATCCAGACCTGGAAGTAATTCAGAGCAAGATTGTTCGTCAGTTAGATATGAAGATTCTTGACGATGAGAATTTAGACGGAAGAGCAAATCATCTACATGCCAGGATACTAAACAAGAAGATTCTTGTGATTCTGGATGATGTTTGGGAACATATTGACTTGGATGCTCTGGGACTTCCTAGTGTCCCTACTTGTAAGATATTGCTGACATCTATAAGTAGAGAAGCATTATCTGATACAAAGATGCAGAAGGTGTTCGCACTCGACATTTTAACAGAAGAAGAGGCTTGGTTTCTGTTTAAGAACAAGGCAGGTGATGTTCTTAAAGAAAACGTTAATATAGTAAGTGTGGCAACTCAAGTTGCTAAAAGATGTGAAGGTTTGCCCATCTCAATTGTCATAGTTGCAAGCTCTTCGAAGGGAATATCTACTTTGACTTCATGGGAAAATGCTTTGAGATGCCTTGAAGAGTTTGATACGGATCACGGGATCACACGAAGCCCACACTTGGGGATAGAGTGGAGTTACAATCAATTGAATGATGAGGAAGTGAAGCAGTTGTTCTTGCTATGTGGATTTGCAATATGGGGAAAAGATATTTATTTGCCAGCCTTGTTGAAATTTAGTATGGGTCTGGGCCTCTTCAGTAAGGCATACACAATGGAGCAAGCAAAAGATGCATTTCAATCATCGGTTGAAAAACTCAAGGATTTTTGTCTACTGACAGACAGTGATGATGATAACAGATATGTCAGAATGCATGACCTTGTACGTGATGTTGCTAACCAGATTGCATTCCGAGATGGACACATCTTATCATCGGCAGAAGATGGGGGTGAGTATGAGTTAAAAGAATGGCTAGATAAGGATTTCTTCAAGAAGTGCAGTATGATGTCTTCACAATCCAGTACTGTTCCTGCGCTCTCTGAAGTAACCAGGATACACCCCAAATTAAAAATGCTTTATTTGCACGGTAAAGGTGATAACTCGGTGGAGATGCCATCCAACTTTTTTAGGGAGATTCAGGAACTCAAAATGTTGGATTTAACCAGACTGCATCTGCTGTCACTACCTTCATCTCTTCAGTTCCTGAAGAATATGCATGCCTTATTTTTAAATCAATGCACCTTGGGAGACGTTGCTTTAGTTGGGCAGCTAAATAACTTAGAAATTCTCAGCTTTTCTCAGTCCAGTCTTGGAGAGACTAGAAGACTTGAGAATGAGAAACAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

619

Amino Acids

70.48

Weight (kDa)

5.89

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 151 - 313 3e-24 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 926
AatII GACGTC 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 205, 926
AccBSI CCGCTC 1 cut(s) 224
AccI GTMKAC 1 cut(s) 1342
AciI CCGC 3 cut(s) 167, 224, 499
AclI AACGTT 1 cut(s) 957
AclWI GGATC 2 cut(s) 1113, 1121
AcsI RAATTY 3 cut(s) 694, 1244, 1794
AcuI CTGAAG 6 cut(s) 332, 503, 1252, 1572, 1691, 1736
AcyI GRCGYC 2 cut(s) 61, 452
AfaI GTAC 5 cut(s) 41, 317, 431, 1395, 1534
AfiI CCNNNNNNNGG 2 cut(s) 1132, 1133
AgsI TTSAA 9 cut(s) 18, 27, 50, 1091, 1162, 1243, 1310, 1322, 1501
AjnI CCWGG 4 cut(s) 432, 633, 725, 1559
AluBI AGCT 7 cut(s) 134, 191, 569, 1035, 1780, 1803, 1855
AluI AGCT 7 cut(s) 134, 191, 569, 1035, 1780, 1803, 1855
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 4 cut(s) 135, 293, 1752, 1820
AlwI GGATC 2 cut(s) 1113, 1121
AlwNI CAGNNNCTG 1 cut(s) 1714
AoxI GGCC 1 cut(s) 1261
ApeKI GCWGC 1 cut(s) 1777
ApoI RAATTY 3 cut(s) 694, 1244, 1794
Asp700I GAANNNNTTC 2 cut(s) 642, 878
AspLEI GCGC 1 cut(s) 1546
AspS9I GGNCC 3 cut(s) 250, 518, 1261
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 4 cut(s) 92, 950, 1472, 1616
AsuII TTCGAA 1 cut(s) 1040
AvaII GGWCC 2 cut(s) 250, 518
BaeGI GKGCMC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 193
BarI GAAGNNNNNNTAC 2 cut(s) 1504, 1536
BbsI GAAGAC 2 cut(s) 1512, 1840
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 1 cut(s) 1789
BccI CCATC 6 cut(s) 117, 257, 1019, 1421, 1448, 1633
BcgI CGANNNNNNTGC 2 cut(s) 1020, 1054
BciT130I CCWGG 4 cut(s) 434, 635, 727, 1561
BciVI GTATCC 3 cut(s) 427, 723, 1557
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 4 cut(s) 135, 293, 1752, 1820
BfaI CTAG 7 cut(s) 329, 492, 522, 570, 804, 1484, 1830
BfuAI ACCTGC 2 cut(s) 205, 926
BfuI GTATCC 3 cut(s) 427, 723, 1557
BisI GCNGC 2 cut(s) 168, 1778
BlsI GCNGC 2 cut(s) 169, 1779
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 5 cut(s) 424, 434, 635, 727, 1561
Bme18I GGWCC 2 cut(s) 250, 518
BmgT120I GGNCC 3 cut(s) 250, 518, 1261
BmrFI CCNGG 5 cut(s) 424, 434, 635, 727, 1561
BmsI GCATC 7 cut(s) 154, 778, 858, 1073, 1291, 1611, 1690
BpiI GAAGAC 2 cut(s) 1512, 1840
Bpu14I TTCGAA 1 cut(s) 1040
BpuEI CTTGAG 3 cut(s) 966, 1313, 1859
BpuMI CCSGG 1 cut(s) 424
BsaAI YACGTR 1 cut(s) 1397
BsaBI GATNNNNATC 2 cut(s) 267, 1104
BsaHI GRCGYC 2 cut(s) 61, 452
BsaJI CCNNGG 4 cut(s) 9, 292, 423, 1752
Bsc4I CCNNNNNNNGG 2 cut(s) 1132, 1133
Bse1I ACTGG 2 cut(s) 1530, 1815
Bse8I GATNNNNATC 2 cut(s) 267, 1104
BseBI CCWGG 4 cut(s) 434, 635, 727, 1561
BseDI CCNNGG 4 cut(s) 9, 292, 423, 1752
BseGI GGATG 5 cut(s) 361, 481, 766, 793, 1625
BseJI GATNNNNATC 2 cut(s) 267, 1104
BseLI CCNNNNNNNGG 2 cut(s) 1132, 1133
BseMII CTCAG 5 cut(s) 126, 147, 248, 1813, 1823
BseNI ACTGG 2 cut(s) 1530, 1815
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 1 cut(s) 1789
BsgI GTGCAG 2 cut(s) 359, 1528
BshFI GGCC 1 cut(s) 1263
BsiHKAI GWGCWC 1 cut(s) 193
BsiSI CCGG 1 cut(s) 423
BslFI GGGAC 2 cut(s) 794, 810
BslI CCNNNNNNNGG 2 cut(s) 1132, 1133
BsmAI GTCTC 4 cut(s) 135, 293, 1752, 1820
BsmBI CGTCTC 1 cut(s) 1752
BsmFI GGGAC 2 cut(s) 794, 810
BsmI GAATGC 2 cut(s) 1386, 1418
BsnI GGCC 1 cut(s) 1263
Bsp119I TTCGAA 1 cut(s) 1040
Bsp1286I GDGCHC 2 cut(s) 193, 499
Bsp143I GATC 5 cut(s) 231, 338, 443, 1105, 1113
Bsp19I CCATGG 2 cut(s) 9, 292
BspACI CCGC 3 cut(s) 167, 224, 499
BspANI GGCC 1 cut(s) 1263
BspCNI CTCAG 5 cut(s) 127, 148, 249, 1812, 1822
BspMI ACCTGC 2 cut(s) 205, 926
BspPI GGATC 2 cut(s) 1113, 1121
BspQI GCTCTTC 2 cut(s) 700, 1042
BspT104I TTCGAA 1 cut(s) 1040
BsrBI CCGCTC 1 cut(s) 224
BsrI ACTGG 2 cut(s) 1530, 1815
BssECI CCNNGG 4 cut(s) 9, 292, 423, 1752
BssMI GATC 5 cut(s) 231, 338, 443, 1105, 1113
BssNI GRCGYC 2 cut(s) 61, 452
BssT1I CCWWGG 3 cut(s) 9, 292, 1752
Bst2UI CCWGG 4 cut(s) 434, 635, 727, 1561
Bst4CI ACNGT 7 cut(s) 46, 238, 320, 460, 1355, 1537, 1598
Bst6I CTCTTC 6 cut(s) 700, 900, 1042, 1086, 1271, 1710
BstACI GRCGYC 2 cut(s) 61, 452
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstBAI YACGTR 1 cut(s) 1397
BstBI TTCGAA 1 cut(s) 1040
BstC8I GCNNGC 7 cut(s) 216, 499, 567, 1033, 1233, 1293, 1728
BstDEI CTNAG 6 cut(s) 135, 156, 257, 1789, 1799, 1809
BstDSI CCRYGG 2 cut(s) 9, 292
BstF5I GGATG 5 cut(s) 361, 481, 766, 793, 1625
BstHHI GCGC 1 cut(s) 1546
BstKTI GATC 5 cut(s) 234, 341, 446, 1108, 1116
BstMAI GTCTC 4 cut(s) 135, 293, 1752, 1820
BstMBI GATC 5 cut(s) 231, 338, 443, 1105, 1113
BstMWI GCNNNNNNNGC 3 cut(s) 197, 1301, 1591
BstNI CCWGG 4 cut(s) 434, 635, 727, 1561
BstNSI RCATGY 2 cut(s) 725, 1730
BstSCI CCNGG 5 cut(s) 422, 432, 633, 725, 1559
BstSLI GKGCMC 1 cut(s) 499
BstV1I GCAGC 1 cut(s) 1789
BstV2I GAAGAC 2 cut(s) 1512, 1840
BsuI GTATCC 3 cut(s) 427, 723, 1557
BsuRI GGCC 1 cut(s) 1263
BtgI CCRYGG 2 cut(s) 9, 292
BtsCI GGATG 5 cut(s) 361, 481, 766, 793, 1625
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 3 cut(s) 82, 234, 1360
BveI ACCTGC 2 cut(s) 205, 926
Cac8I GCNNGC 7 cut(s) 216, 499, 567, 1033, 1233, 1293, 1728
CaiI CAGNNNCTG 1 cut(s) 1714
CfoI GCGC 1 cut(s) 1546
Cfr13I GGNCC 3 cut(s) 250, 518, 1261
CseI GACGC 1 cut(s) 69
Csp6I GTAC 5 cut(s) 40, 316, 430, 1394, 1533
CspCI CAANNNNNGTGG 2 cut(s) 389, 424
CviAII CATG 7 cut(s) 10, 293, 472, 722, 1065, 1385, 1727
CviQI GTAC 5 cut(s) 40, 316, 430, 1394, 1533
DdeI CTNAG 6 cut(s) 135, 156, 257, 1789, 1799, 1809
DpnI GATC 5 cut(s) 233, 340, 445, 1107, 1115
DpnII GATC 5 cut(s) 231, 338, 443, 1105, 1113
DraI TTTAAA 1 cut(s) 1740
Eam1104I CTCTTC 6 cut(s) 700, 900, 1042, 1086, 1271, 1710
EarI CTCTTC 6 cut(s) 700, 900, 1042, 1086, 1271, 1710
Ecl136II GAGCTC 1 cut(s) 191
Eco130I CCWWGG 3 cut(s) 9, 292, 1752
Eco24I GRGCYC 1 cut(s) 193
Eco47I GGWCC 2 cut(s) 250, 518
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 6 cut(s) 332, 503, 1252, 1572, 1691, 1736
EcoICRI GAGCTC 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 4 cut(s) 432, 633, 725, 1559
EcoT14I CCWWGG 3 cut(s) 9, 292, 1752
EcoT22I ATGCAT 3 cut(s) 1306, 1386, 1728
EcoT38I GRGCYC 1 cut(s) 193
ErhI CCWWGG 3 cut(s) 9, 292, 1752
Esp3I CGTCTC 1 cut(s) 1752
FaeI CATG 7 cut(s) 13, 296, 475, 725, 1068, 1388, 1730
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 2 cut(s) 794, 810
FatI CATG 7 cut(s) 9, 292, 471, 721, 1064, 1384, 1726
FauI CCCGC 1 cut(s) 506
FblI GTMKAC 1 cut(s) 1342
Fnu4HI GCNGC 2 cut(s) 168, 1778
FokI GGATG 5 cut(s) 368, 488, 773, 800, 1612
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 2 cut(s) 168, 1778
FspBI CTAG 7 cut(s) 329, 492, 522, 570, 804, 1484, 1830
GlaI GCGC 1 cut(s) 1545
GluI GCNGC 2 cut(s) 168, 1778
HaeIII GGCC 1 cut(s) 1263
HapII CCGG 1 cut(s) 423
HgaI GACGC 1 cut(s) 69
HhaI GCGC 1 cut(s) 1546
Hin1I GRCGYC 2 cut(s) 61, 452
Hin1II CATG 7 cut(s) 13, 296, 475, 725, 1068, 1388, 1730
Hin6I GCGC 1 cut(s) 1544
HinP1I GCGC 1 cut(s) 1544
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HinfI GANTC 5 cut(s) 14, 679, 745, 754, 1645
HpaII CCGG 1 cut(s) 423
HphI GGTGA 4 cut(s) 92, 950, 1472, 1616
Hpy166II GTNNAC 4 cut(s) 42, 456, 518, 1343
Hpy188I TCNGA 7 cut(s) 258, 312, 648, 859, 1379, 1424, 1552
Hpy188III TCNNGA 8 cut(s) 281, 619, 629, 683, 758, 1501, 1649, 1714
Hpy8I GTNNAC 4 cut(s) 42, 456, 518, 1343
HpyAV CCTTC 6 cut(s) 107, 263, 868, 995, 1036, 1707
HpyCH4III ACNGT 7 cut(s) 46, 238, 320, 460, 1355, 1537, 1598
HpyCH4IV ACGT 4 cut(s) 452, 957, 1396, 1761
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 1301, 1591
HpyF3I CTNAG 6 cut(s) 135, 156, 257, 1789, 1799, 1809
HpySE526I ACGT 4 cut(s) 452, 957, 1396, 1761
Hsp92I GRCGYC 2 cut(s) 61, 452
Hsp92II CATG 7 cut(s) 13, 296, 475, 725, 1068, 1388, 1730
HspAI GCGC 1 cut(s) 1544
Kzo9I GATC 5 cut(s) 231, 338, 443, 1105, 1113
LguI GCTCTTC 2 cut(s) 700, 1042
LmnI GCTCC 1 cut(s) 1288
Lsp1109I GCAGC 1 cut(s) 1789
LweI GCATC 7 cut(s) 154, 778, 858, 1073, 1291, 1611, 1690
MaeI CTAG 7 cut(s) 329, 492, 522, 570, 804, 1484, 1830
MaeII ACGT 4 cut(s) 452, 957, 1396, 1761
MaeIII GTNAC 3 cut(s) 1148, 1555, 1689
MalI GATC 5 cut(s) 233, 340, 445, 1107, 1115
MbiI CCGCTC 1 cut(s) 224
MboI GATC 5 cut(s) 231, 338, 443, 1105, 1113
MfeI CAATTG 2 cut(s) 1017, 1157
MhlI GDGCHC 2 cut(s) 193, 499
MmeI TCCRAC 3 cut(s) 37, 1644, 1653
MnlI CCTC 8 cut(s) 151, 220, 241, 456, 518, 901, 1162, 1274
Mph1103I ATGCAT 3 cut(s) 1306, 1386, 1728
MroXI GAANNNNTTC 2 cut(s) 642, 878
MslI CAYNNNNRTG 2 cut(s) 239, 720
MspI CCGG 1 cut(s) 423
MspR9I CCNGG 5 cut(s) 424, 434, 635, 727, 1561
MunI CAATTG 2 cut(s) 1017, 1157
Mva1269I GAATGC 2 cut(s) 1386, 1418
MvaI CCWGG 4 cut(s) 434, 635, 727, 1561
MwoI GCNNNNNNNGC 3 cut(s) 197, 1301, 1591
NciI CCSGG 1 cut(s) 424
NcoI CCATGG 2 cut(s) 9, 292
NdeII GATC 5 cut(s) 231, 338, 443, 1105, 1113
NlaIII CATG 7 cut(s) 13, 296, 475, 725, 1068, 1388, 1730
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 1689
NsiI ATGCAT 3 cut(s) 1306, 1386, 1728
NspI RCATGY 2 cut(s) 725, 1730
NspV TTCGAA 1 cut(s) 1040
PaeI GCATGC 1 cut(s) 1730
PaqCI CACCTGC 1 cut(s) 926
PciSI GCTCTTC 2 cut(s) 700, 1042
PctI GAATGC 2 cut(s) 1386, 1418
PdmI GAANNNNTTC 2 cut(s) 642, 878
PfeI GAWTC 5 cut(s) 14, 679, 745, 754, 1645
PkrI GCNGC 2 cut(s) 169, 1779
Ppu21I YACGTR 1 cut(s) 1397
PpuMI RGGWCCY 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 193
Psp1406I AACGTT 1 cut(s) 957
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 4 cut(s) 432, 633, 725, 1559
PspGI CCWGG 4 cut(s) 432, 633, 725, 1559
PspPI GGNCC 3 cut(s) 250, 518, 1261
PspPPI RGGWCCY 1 cut(s) 250
PstNI CAGNNNCTG 1 cut(s) 1714
RsaI GTAC 5 cut(s) 41, 317, 431, 1395, 1534
RsaNI GTAC 5 cut(s) 40, 316, 430, 1394, 1533
RseI CAYNNNNRTG 2 cut(s) 239, 720
SacI GAGCTC 1 cut(s) 193
SapI GCTCTTC 2 cut(s) 700, 1042
SatI GCNGC 2 cut(s) 168, 1778
Sau3AI GATC 5 cut(s) 231, 338, 443, 1105, 1113
Sau96I GGNCC 3 cut(s) 250, 518, 1261
ScaI AGTACT 1 cut(s) 1534
ScrFI CCNGG 5 cut(s) 424, 434, 635, 727, 1561
SduI GDGCHC 2 cut(s) 193, 499
SfaNI GCATC 7 cut(s) 154, 778, 858, 1073, 1291, 1611, 1690
SfuI TTCGAA 1 cut(s) 1040
SinI GGWCC 2 cut(s) 250, 518
SmiMI CAYNNNNRTG 2 cut(s) 239, 720
SmlI CTYRAG 3 cut(s) 981, 1328, 1838
SmoI CTYRAG 3 cut(s) 981, 1328, 1838
SphI GCATGC 1 cut(s) 1730
SsiI CCGC 3 cut(s) 167, 224, 499
SspMI CTAG 7 cut(s) 329, 492, 522, 570, 804, 1484, 1830
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 5 cut(s) 422, 432, 633, 725, 1559
StyI CCWWGG 3 cut(s) 9, 292, 1752
TaaI ACNGT 7 cut(s) 46, 238, 320, 460, 1355, 1537, 1598
TaiI ACGT 4 cut(s) 455, 960, 1399, 1764
TaqI TCGA 4 cut(s) 271, 442, 888, 1040
TatI WGTACW 2 cut(s) 39, 1532
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 5 cut(s) 14, 679, 745, 754, 1645
TscAI CASTG 3 cut(s) 89, 241, 1360
TseFI GTSAC 1 cut(s) 1689
TseI GCWGC 1 cut(s) 1777
Tsp45I GTSAC 1 cut(s) 1689
TspDTI ATGAA 5 cut(s) 198, 492, 689, 1053, 1689
TspGWI ACGGA 2 cut(s) 717, 1118
TspRI CASTG 3 cut(s) 89, 241, 1360
VpaK11BI GGWCC 2 cut(s) 250, 518
XapI RAATTY 3 cut(s) 694, 1244, 1794
XceI RCATGY 2 cut(s) 725, 1730
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 1342
XmnI GAANNNNTTC 2 cut(s) 642, 878
XspI CTAG 7 cut(s) 329, 492, 522, 570, 804, 1484, 1830
ZraI GACGTC 1 cut(s) 453
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 3 cut(s) 1306, 1386, 1728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.