Rh2DG234400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003701
Physical Location & Seq
Reverse (-)
23306983 .. 23313065
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG234400.1

Sequence Viewer

Length: 3924 bp
ATGGAAGCCATGGATTCAATTTTTTCAAAAATTGCCGAGTACACAGTTGAACCCGTTGGACGCCAAGTGGGTTATGTAATTCACTGCAACAGAAACCTTTGCATCCTACATTTTATAGTGGAAGAGTTAAAAGCTGAGAGAGACGAGTTAAAAACTGAGGTTGATGCGGCTGAAATAATAAGTGAACGAGTTCAACCTGCTTTACAGGATTGGCTGGCAAGAGCGGAGGCGATCACTGTAATAGCAGAGGACCTTCTGAGAGATGGAAATCGAGCAAATCTGGAATGTCTCCATGGGTTTTGTCCTAATCTGAAGTACCGTTATCGGCTAGGCAGGAAATCAACAAGATTGCTGGATGAGATTGAGATGTTCCTGCACATAAAAGAAGCACTAATCTTGCAAATGGAGTGGGGTGTATCCACCGGGGAGTACCAGGCTTTTTGTTCAAGGATGTCAACTGTGAGGCAAATCATGGATGAACTGAAGAAGTCTAGAGCCCGCAGGATTGGGGTGTGTGGACCTAGAGGTGTGGGTAAAACTCTACTTGCCAAAGAAGTTTATAGGCAAGCTAGAAAAGATAAAAAGTTATTTGATGATGTGGTTATCCTATTAGATGTCAAGAAAAATCCAGACCTGGAAGTAATTCAGAGCAAGATTGTTCGTCAGTTAGATATGAAGATTCTTGACGATGAGAATTTAGACGGAAGAGCAAATCGTCTACGTGCCAGGATACTAAACAAGAAGATTCTTGTGATTCTGGATGATGTTTGGGAACATATTGACTTGGATTCTCTGGGACTTCCTAGTGTCCCTACTTGTAAGATATTGCTGACATCTATAAGTAGAGAAGCATTATCTGATACAAAGATGCAGAAGGTGTTCGCACTAGACATTTTAACAGAAGAAGAGGCTTGGTTTCTGTTTAAGAACAAGGCAGGTGATGTTCTTAAAGAAAACGTTAATATAGTAAGTGTGGCAACTCAAGTTGCTAAAAGATGTGAAGGTTTGCCCATCTCAATTGTCATAGTTGCAAGCTCTTTGAAGAGAATATCTACTTTAACTGCATGGGAAAATGCTTTGAGATGCCTTGAAGAGTTTGATACAGATCACGGGATCATACGAAGCCCACACTTGGGGATAGAGTGGAGTTACAAGCAATTGAGAGATGAGGAAGTGAAGCAGTTGTTCTTGCTATGTGGATTTGCAATATGGGGAAAAGATATTTATTTGCCAGCCTTGTTGAAATTTAGTATGGGTTTGGGCCTCTTCAGTAAGGCATGCACAATGGAGCAAGCAAAAGATGCATTTAAATCATCGGTTGAAAAGCTCAAGGATTTTTATCTACTGACAGACAGTGATGATGATAACAGATATGTCAGAATGCATGACCTTGTACGTGATGTTGCTAAATGGATTGCAATCCGAGATGGATGCATCTTGTCATCGACCGAAGATAGGGATGAGTATCAGTTAAAAGAATGGCTAGATAAGGATTTCTTCAAAAAGTGCAGTAAGATGTCTTCACAATCCAGTACTGTTCCTGCGCTATCTGAAGTAACCAGGATACACCCCAAATTAAAAATGCTTTATTTGCACGGTAAAGGTGATAACTCGGTGGAGATGCCATCCAACTTTTTTAGGGAGATTCAGGAACTCAAAATGTTGGATTTAACCAGACTGCATCTGCTGTCACTACCTTCATCTCTTCAGTTCCTGAAGAATATGCATGCCTTATTTTTAAATCAATGCACCTTGGGAGACGTTGCTTTAGTTGGGCAGCTAAGTAACTTAGAAATTCTCAGCTTTTCTCAGTCCAGTGTCAAACAATTGCCTAAAGAAATAGGGCATTTGACTCGTCTTCGGTTGTTGGATTTATCTGATTGCTCTGAACTTGAAGTTATTTCACCCAATGTCATATCAAGCTTGGAGAGACTAGAAGACTTGAGAATGAGAAACAGCTTTAACAAGTGGGAGGTTGGAGGTAAAAGAAGCAACGCAAGCCTTTCTGAGCTGAAGTATTTGTCTCAGTTATCCATATTACAGATACATATTCTGAATGCTGACATTCTTCCAGCAAACTTGTTCTCGCGCAAGTTAGAAAGATTCAATGTGTTTATTGGGGATGAGTGGGTTGAGAGATATACTCGAAAGACACTGCTAATGGTTGAGGCGACACTCAACACACTAAAACTCAAGCCCACTACCAGCACTGAAGGATTGGATACAGGACTAAAATTGTTGCTGAAGATAACTGAAGACTTGTCCTTGGATGGAACAGAGGGTGTCAATAAGGTGCTCCATCAATTAGATACTCAAGACTTGGAGCATTTGAAACATTTGCATGTCCAAAACAATGTTGATATAAGATATCTCATTAACAGGGAGGTTCTGTTGCGTAACTTAATAAGCTTCACTGTTCATCGATGTGATGGTTCAAGTTTCTTATTGTCATCTTCTATGGCAAGAAGTTTTGTGCAACTCAAACATCTTGTAATATCAGAATGTCAAATCATGGAAGAGATACTATCGACAAGAGAATACAGTGAAGAAAACAAGGATAACATGTTTCCGAAGTTGGAGAAATTGGAACTAAGTGATCTTCCTCATCTCACTAGATTCTGCTCAGGAGGTTATGTTGAATTTGCATCTTTGGAGAGATTGCAACTAGAGAAGTGTACTAAGTTGGAGACTTCCATCTCTAATCATGAGAGTGCTGCACCATATTTTCTTTTTGACGAGAAGGTAGGGTTTCCAAGGTTGGAGATATTGACCGTCAATGACTTACCTAAGCTGACAACAATGTGGCACACCCAACTTGCTCGAAACTCTTTTTGCAAACTCAGGGGCCTTACAGTTCAACGATGCAATAGTCTTAAAAGCATTTTTCCAGACTCGATAATAGCTGGAGCTTTTCAGGAGCTACAAAATTTGACAGTGGAGGACTGTGTAGTGGAGGAAATAGTTGCCAAGGAAGAGGGACTAGAAAAACAAAACCACGATAAGTTCTTCCCAAAGCTAGAGTGTTTGGAACTTAAAAGTCTATCCCAACTTAAGAGATTCTATCCAAGTATGCCTGCTTTCAGTTGGCCACACCTCAAAACATTACTGTTGTTTGATTGTCAGCAGGTGGATATTTTTACCCCGGAATTTTCAGGCATTGAGAAAACACATGACTCGGGCAGTCAGTCTATTCCAGTTAAACAATCTTCTGTTCTGATAGATAAGACCTCATTCCCCAACCTAGAAGATTTGACCGTAGTCTGCATGGAAAATTGGCACAGCCGACCTGCCCAATCAGTGTTGTGCCCGAAAGCTGTTTTCTCTGGGATCAGTAGTAGACATCCAGATGGCGGATCCCTTCTACATTTAAGAACTTTGAGGCTGCATGGAATGAAGAAGCCTATGCGTCTCTGGGACACCACCGAATCAGCTGGCCCGGATTTTCCAAAGTTGGAAATTCTAGAGCTGAAGTTTTGTGGATTGAATAATCTAGACTTATCTGCAATATCCTTCCAGAATCTAACCATTTTAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGGCAACTTACACGGTTGCTCAAGGTTTAGTGAGACTCAAAAAATTGAAAGTTGACGAGTGTAAAAATATGAAAGAAATCTTCACAAGCGAAGGAATGGAAGAAGATGCAAGTGAATGTAAGATTGTTTTCAGCCGGTTGCAACATTTGGAACTTACTGATTTAATAAGTTTGGAACGCTTTTGCCCGAGAAATTGCAGGGTGAAGGTCCCAGCAACCTTGGGAACTTTAGAGGTAAAATGGTGCTCTTTCGAGCTGAAGATTTCCTCCGATGGGGTACTAGAAGAAATTGTTCCCAATATGCATCTAGATGTACAAAAGTCACATCCCAAAGAAGAGGTAAAGCGGAAACCTATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1307

Amino Acids

149.55

Weight (kDa)

6.27

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 153 - 313 4e-24 NB-ARC domain
LRR_14 PF23598 527 - 681 1.1e-08 Leucine-rich repeat region
LRR_14 PF23598 591 - 865 1.7e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 796 - 897 2.9e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 922 - 1043 3.1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1163 - 1254 4.9e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 926, 3115
Acc36I ACCTGC 4 cut(s) 205, 926, 3115, 3295
AccBSI CCGCTC 1 cut(s) 224
AccI GTMKAC 2 cut(s) 718, 3337
AccII CGCG 1 cut(s) 2089
AciI CCGC 5 cut(s) 167, 224, 499, 3351, 3907
AclI AACGTT 1 cut(s) 957
AclWI GGATC 4 cut(s) 1121, 3335, 3348, 3361
AcoI YGGCCR 1 cut(s) 3086
AcsI RAATTY 7 cut(s) 694, 1244, 1794, 2639, 2926, 3146, 3456
AcyI GRCGYC 1 cut(s) 61
AfaI GTAC 8 cut(s) 41, 317, 431, 1395, 1534, 2677, 3840, 3876
AfiI CCNNNNNNNGG 5 cut(s) 1132, 1133, 1455, 3350, 3834
AflII CTTAAG 1 cut(s) 3050
AflIII ACRYGT 1 cut(s) 2562
AjnI CCWGG 4 cut(s) 432, 633, 725, 1559
Alw21I GWGCWC 2 cut(s) 2298, 3809
Alw26I GTCTC 8 cut(s) 135, 293, 1752, 1924, 2028, 2681, 3413, 3589
AlwI GGATC 4 cut(s) 1121, 3335, 3348, 3361
AlwNI CAGNNNCTG 1 cut(s) 1714
Ama87I CYCGRG 2 cut(s) 3175, 3748
AoxI GGCC 4 cut(s) 1261, 2845, 3086, 3433
ApeKI GCWGC 3 cut(s) 1777, 2714, 3382
ApoI RAATTY 7 cut(s) 694, 1244, 1794, 2639, 2926, 3146, 3456
Asp700I GAANNNNTTC 4 cut(s) 189, 642, 878, 3852
AspLEI GCGC 2 cut(s) 1546, 2091
AspS9I GGNCC 6 cut(s) 250, 518, 1261, 2845, 3434, 3769
AsuC2I CCSGG 3 cut(s) 424, 3143, 3437
AsuHPI GGTGA 4 cut(s) 950, 1616, 1896, 3775
AvaI CYCGRG 2 cut(s) 3175, 3748
AvaII GGWCC 3 cut(s) 250, 518, 3769
BaeGI GKGCMC 1 cut(s) 3308
BalI TGGCCA 1 cut(s) 3088
BamHI GGATCC 1 cut(s) 3353
BanII GRGCYC 1 cut(s) 499
BarI GAAGNNNNNNTAC 2 cut(s) 1504, 1536
BbsI GAAGAC 4 cut(s) 1512, 1850, 1944, 2262
Bbv12I GWGCWC 2 cut(s) 2298, 3809
BbvI GCAGC 3 cut(s) 1789, 2701, 3369
BcgI CGANNNNNNTGC 4 cut(s) 1413, 1447, 1835, 1869
BciT130I CCWGG 4 cut(s) 434, 635, 727, 1561
BciVI GTATCC 4 cut(s) 427, 723, 1557, 2215
BcnI CCSGG 3 cut(s) 424, 3143, 3437
BcoDI GTCTC 8 cut(s) 135, 293, 1752, 1924, 2028, 2681, 3413, 3589
BfrI CTTAAG 1 cut(s) 3050
BfuAI ACCTGC 4 cut(s) 205, 926, 3115, 3295
BfuI GTATCC 4 cut(s) 427, 723, 1557, 2215
BisI GCNGC 4 cut(s) 168, 1778, 2715, 3383
BlsI GCNGC 4 cut(s) 169, 1779, 2716, 3384
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 7 cut(s) 424, 434, 635, 727, 1561, 3143, 3437
Bme18I GGWCC 3 cut(s) 250, 518, 3769
BmeT110I CYCGRG 2 cut(s) 3175, 3748
BmgT120I GGNCC 6 cut(s) 250, 518, 1261, 2845, 3434, 3769
BmiI GGNNCC 3 cut(s) 2846, 3355, 3771
BmrFI CCNGG 7 cut(s) 424, 434, 635, 727, 1561, 3143, 3437
BoxI GACNNNNGTC 1 cut(s) 3257
BpiI GAAGAC 4 cut(s) 1512, 1850, 1944, 2262
BplI GAGNNNNNCTC 2 cut(s) 2128, 2160
BpmI CTGGAG 1 cut(s) 2925
Bpu10I CCTNAGC 2 cut(s) 2623, 2787
BpuEI CTTGAG 6 cut(s) 966, 1313, 1963, 2177, 2298, 3567
BpuMI CCSGG 3 cut(s) 424, 3143, 3437
Bsa29I ATCGAT 1 cut(s) 2422
BsaAI YACGTR 2 cut(s) 722, 1397
BsaBI GATNNNNATC 5 cut(s) 267, 1104, 1338, 1418, 1464
BsaHI GRCGYC 1 cut(s) 61
BsaJI CCNNGG 9 cut(s) 9, 292, 423, 1752, 2265, 2753, 2967, 3141, 3780
BsaXI ACNNNNNCTCC 4 cut(s) 2375, 2405, 2619, 2649
Bsc4I CCNNNNNNNGG 5 cut(s) 1132, 1133, 1455, 3350, 3834
Bse118I RCCGGY 1 cut(s) 3696
Bse1I ACTGG 3 cut(s) 1530, 1815, 3194
Bse8I GATNNNNATC 5 cut(s) 267, 1104, 1338, 1418, 1464
BseBI CCWGG 4 cut(s) 434, 635, 727, 1561
BseCI ATCGAT 1 cut(s) 2422
BseDI CCNNGG 9 cut(s) 9, 292, 423, 1752, 2265, 2753, 2967, 3141, 3780
BseJI GATNNNNATC 5 cut(s) 267, 1104, 1338, 1418, 1464
BseLI CCNNNNNNNGG 5 cut(s) 1132, 1133, 1455, 3350, 3834
BseMII CTCAG 9 cut(s) 126, 147, 248, 1813, 1823, 1998, 2039, 2637, 2854
BseNI ACTGG 3 cut(s) 1530, 1815, 3194
BseSI GKGCMC 1 cut(s) 3308
BseXI GCAGC 3 cut(s) 1789, 2701, 3369
BseYI CCCAGC 1 cut(s) 3772
BsgI GTGCAG 3 cut(s) 359, 1528, 2700
Bsh1236I CGCG 1 cut(s) 2089
Bsh1285I CGRYCG 1 cut(s) 1449
BshFI GGCC 4 cut(s) 1263, 2847, 3088, 3435
BshVI ATCGAT 1 cut(s) 2422
BsiEI CGRYCG 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 2298, 3809
BsiHKCI CYCGRG 2 cut(s) 3175, 3748
BsiSI CCGG 4 cut(s) 423, 3143, 3437, 3697
BslFI GGGAC 5 cut(s) 794, 810, 2991, 3428, 3755
BslI CCNNNNNNNGG 5 cut(s) 1132, 1133, 1455, 3350, 3834
BsmAI GTCTC 8 cut(s) 135, 293, 1752, 1924, 2028, 2681, 3413, 3589
BsmBI CGTCTC 3 cut(s) 135, 1752, 3413
BsmFI GGGAC 5 cut(s) 794, 810, 2991, 3428, 3755
BsmI GAATGC 2 cut(s) 1386, 2062
BsnI GGCC 4 cut(s) 1263, 2847, 3088, 3435
BsoBI CYCGRG 2 cut(s) 3175, 3748
Bsp1286I GDGCHC 4 cut(s) 499, 2298, 3308, 3809
Bsp1407I TGTACA 1 cut(s) 3874
Bsp143I GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
Bsp19I CCATGG 2 cut(s) 9, 292
BspACI CCGC 5 cut(s) 167, 224, 499, 3351, 3907
BspANI GGCC 4 cut(s) 1263, 2847, 3088, 3435
BspCNI CTCAG 9 cut(s) 127, 148, 249, 1812, 1822, 1999, 2038, 2636, 2853
BspDI ATCGAT 1 cut(s) 2422
BspFNI CGCG 1 cut(s) 2089
BspHI TCATGA 1 cut(s) 2704
BspLI GGNNCC 3 cut(s) 2846, 3355, 3771
BspMI ACCTGC 4 cut(s) 205, 926, 3115, 3295
BspPI GGATC 4 cut(s) 1121, 3335, 3348, 3361
BspQI GCTCTTC 1 cut(s) 700
BspTI CTTAAG 1 cut(s) 3050
BsrBI CCGCTC 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 3696
BsrGI TGTACA 1 cut(s) 3874
BsrI ACTGG 3 cut(s) 1530, 1815, 3194
BssAI RCCGGY 1 cut(s) 3696
BssECI CCNNGG 9 cut(s) 9, 292, 423, 1752, 2265, 2753, 2967, 3141, 3780
BssMI GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
BssNI GRCGYC 1 cut(s) 61
BssT1I CCWWGG 7 cut(s) 9, 292, 1752, 2265, 2753, 2967, 3780
Bst2UI CCWGG 4 cut(s) 434, 635, 727, 1561
BstACI GRCGYC 1 cut(s) 61
BstAFI CTTAAG 1 cut(s) 3050
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstAUI TGTACA 1 cut(s) 3874
BstBAI YACGTR 2 cut(s) 722, 1397
BstDSI CCRYGG 2 cut(s) 9, 292
BstFNI CGCG 1 cut(s) 2089
BstHHI GCGC 2 cut(s) 1546, 2091
BstKTI GATC 6 cut(s) 234, 1108, 1116, 2599, 3330, 3356
BstMAI GTCTC 8 cut(s) 135, 293, 1752, 1924, 2028, 2681, 3413, 3589
BstMBI GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
BstMCI CGRYCG 1 cut(s) 1449
BstMWI GCNNNNNNNGC 3 cut(s) 1301, 1591, 1998
BstNI CCWGG 4 cut(s) 434, 635, 727, 1561
BstNSI RCATGY 4 cut(s) 1281, 1730, 2345, 2566
BstPAI GACNNNNGTC 1 cut(s) 3257
BstSCI CCNGG 7 cut(s) 422, 432, 633, 725, 1559, 3141, 3435
BstSLI GKGCMC 1 cut(s) 3308
BstUI CGCG 1 cut(s) 2089
BstV1I GCAGC 3 cut(s) 1789, 2701, 3369
BstV2I GAAGAC 4 cut(s) 1512, 1850, 1944, 2262
BstX2I RGATCY 1 cut(s) 3353
BstYI RGATCY 1 cut(s) 3353
Bsu15I ATCGAT 1 cut(s) 2422
BsuI GTATCC 4 cut(s) 427, 723, 1557, 2215
BsuRI GGCC 4 cut(s) 1263, 2847, 3088, 3435
BsuTUI ATCGAT 1 cut(s) 2422
BtgI CCRYGG 2 cut(s) 9, 292
BtsI GCAGTG 2 cut(s) 82, 2153
BveI ACCTGC 4 cut(s) 205, 926, 3115, 3295
CaiI CAGNNNCTG 1 cut(s) 1714
CciI TCATGA 1 cut(s) 2704
CfoI GCGC 2 cut(s) 1546, 2091
Cfr10I RCCGGY 1 cut(s) 3696
Cfr13I GGNCC 6 cut(s) 250, 518, 1261, 2845, 3434, 3769
ClaI ATCGAT 1 cut(s) 2422
CseI GACGC 2 cut(s) 69, 3395
Csp6I GTAC 8 cut(s) 40, 316, 430, 1394, 1533, 2676, 3839, 3875
CspCI CAANNNNNGTGG 2 cut(s) 389, 424
CviQI GTAC 8 cut(s) 40, 316, 430, 1394, 1533, 2676, 3839, 3875
DpnI GATC 6 cut(s) 233, 1107, 1115, 2598, 3329, 3355
DpnII GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
DraI TTTAAA 2 cut(s) 1309, 1740
EaeI YGGCCR 1 cut(s) 3086
EciI GGCGGA 1 cut(s) 3366
Eco130I CCWWGG 7 cut(s) 9, 292, 1752, 2265, 2753, 2967, 3780
Eco24I GRGCYC 1 cut(s) 499
Eco32I GATATC 1 cut(s) 2369
Eco47I GGWCC 3 cut(s) 250, 518, 3769
Eco88I CYCGRG 2 cut(s) 3175, 3748
EcoO109I RGGNCCY 3 cut(s) 250, 2845, 3769
EcoRII CCWGG 4 cut(s) 432, 633, 725, 1559
EcoRV GATATC 1 cut(s) 2369
EcoT14I CCWWGG 7 cut(s) 9, 292, 1752, 2265, 2753, 2967, 3780
EcoT22I ATGCAT 5 cut(s) 1306, 1386, 1436, 1728, 3867
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 7 cut(s) 9, 292, 1752, 2265, 2753, 2967, 3780
Esp3I CGTCTC 3 cut(s) 135, 1752, 3413
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 5 cut(s) 794, 810, 2991, 3428, 3755
FauI CCCGC 1 cut(s) 506
FblI GTMKAC 2 cut(s) 718, 3337
Fnu4HI GCNGC 4 cut(s) 168, 1778, 2715, 3383
FriOI GRGCYC 1 cut(s) 499
Fsp4HI GCNGC 4 cut(s) 168, 1778, 2715, 3383
GlaI GCGC 2 cut(s) 1545, 2090
GluI GCNGC 4 cut(s) 168, 1778, 2715, 3383
GsaI CCCAGC 1 cut(s) 3776
GsuI CTGGAG 1 cut(s) 2925
HaeIII GGCC 4 cut(s) 1263, 2847, 3088, 3435
HapII CCGG 4 cut(s) 423, 3143, 3437, 3697
HgaI GACGC 2 cut(s) 69, 3395
HhaI GCGC 2 cut(s) 1546, 2091
Hin1I GRCGYC 1 cut(s) 61
Hin6I GCGC 2 cut(s) 1544, 2089
HinP1I GCGC 2 cut(s) 1544, 2089
HincII GTYRAC 2 cut(s) 456, 3616
HindII GTYRAC 2 cut(s) 456, 3616
HindIII AAGCTT 2 cut(s) 1921, 2407
HpaII CCGG 4 cut(s) 423, 3143, 3437, 3697
HphI GGTGA 4 cut(s) 950, 1616, 1896, 3775
Hpy166II GTNNAC 8 cut(s) 42, 185, 456, 518, 719, 2676, 3338, 3616
Hpy8I GTNNAC 8 cut(s) 42, 185, 456, 518, 719, 2676, 3338, 3616
HpyCH4IV ACGT 4 cut(s) 721, 957, 1396, 1761
HpyF10VI GCNNNNNNNGC 3 cut(s) 1301, 1591, 1998
HpySE526I ACGT 4 cut(s) 721, 957, 1396, 1761
Hsp92I GRCGYC 1 cut(s) 61
HspAI GCGC 2 cut(s) 1544, 2089
Kzo9I GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
LguI GCTCTTC 1 cut(s) 700
LmnI GCTCC 5 cut(s) 1288, 2301, 2323, 2906, 2917
Lsp1109I GCAGC 3 cut(s) 1789, 2701, 3369
MaeII ACGT 4 cut(s) 721, 957, 1396, 1761
MaeIII GTNAC 7 cut(s) 1148, 1555, 1689, 1784, 2396, 3535, 3882
MalI GATC 6 cut(s) 233, 1107, 1115, 2598, 3329, 3355
MbiI CCGCTC 1 cut(s) 224
MboI GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
MfeI CAATTG 3 cut(s) 1017, 1157, 1826
MflI RGATCY 1 cut(s) 3353
MhlI GDGCHC 4 cut(s) 499, 2298, 3308, 3809
MlsI TGGCCA 1 cut(s) 3088
MluNI TGGCCA 1 cut(s) 3088
MlyI GAGTC 4 cut(s) 1846, 2885, 3167, 3591
MmeI TCCRAC 9 cut(s) 37, 1644, 1653, 1848, 1957, 2556, 2664, 2739, 3432
Mox20I TGGCCA 1 cut(s) 3088
Mph1103I ATGCAT 5 cut(s) 1306, 1386, 1436, 1728, 3867
MroXI GAANNNNTTC 4 cut(s) 189, 642, 878, 3852
MscI TGGCCA 1 cut(s) 3088
MslI CAYNNNNRTG 5 cut(s) 2340, 2424, 2709, 3345, 3870
Msp20I TGGCCA 1 cut(s) 3088
MspA1I CMGCKG 1 cut(s) 3431
MspCI CTTAAG 1 cut(s) 3050
MspI CCGG 4 cut(s) 423, 3143, 3437, 3697
MspR9I CCNGG 7 cut(s) 424, 434, 635, 727, 1561, 3143, 3437
MunI CAATTG 3 cut(s) 1017, 1157, 1826
Mva1269I GAATGC 2 cut(s) 1386, 2062
MvaI CCWGG 4 cut(s) 434, 635, 727, 1561
MvnI CGCG 1 cut(s) 2089
MwoI GCNNNNNNNGC 3 cut(s) 1301, 1591, 1998
NciI CCSGG 3 cut(s) 424, 3143, 3437
NcoI CCATGG 2 cut(s) 9, 292
NdeII GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
NlaIV GGNNCC 3 cut(s) 2846, 3355, 3771
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 2 cut(s) 1689, 3882
NsiI ATGCAT 5 cut(s) 1306, 1386, 1436, 1728, 3867
NspI RCATGY 4 cut(s) 1281, 1730, 2345, 2566
PaeI GCATGC 2 cut(s) 1281, 1730
PagI TCATGA 1 cut(s) 2704
PaqCI CACCTGC 2 cut(s) 926, 3115
PciI ACATGT 1 cut(s) 2562
PciSI GCTCTTC 1 cut(s) 700
PctI GAATGC 2 cut(s) 1386, 2062
PdmI GAANNNNTTC 4 cut(s) 189, 642, 878, 3852
PkrI GCNGC 4 cut(s) 169, 1779, 2716, 3384
PleI GAGTC 4 cut(s) 1846, 2885, 3167, 3591
PpsI GAGTC 4 cut(s) 1846, 2885, 3167, 3591
Ppu21I YACGTR 2 cut(s) 722, 1397
PpuMI RGGWCCY 2 cut(s) 250, 3769
PscI ACATGT 1 cut(s) 2562
PshAI GACNNNNGTC 1 cut(s) 3257
Psp1406I AACGTT 1 cut(s) 957
Psp5II RGGWCCY 2 cut(s) 250, 3769
Psp6I CCWGG 4 cut(s) 432, 633, 725, 1559
PspFI CCCAGC 1 cut(s) 3772
PspGI CCWGG 4 cut(s) 432, 633, 725, 1559
PspN4I GGNNCC 3 cut(s) 2846, 3355, 3771
PspPI GGNCC 6 cut(s) 250, 518, 1261, 2845, 3434, 3769
PspPPI RGGWCCY 2 cut(s) 250, 3769
PstNI CAGNNNCTG 1 cut(s) 1714
PsuI RGATCY 1 cut(s) 3353
PvuII CAGCTG 1 cut(s) 3431
RsaI GTAC 8 cut(s) 41, 317, 431, 1395, 1534, 2677, 3840, 3876
RsaNI GTAC 8 cut(s) 40, 316, 430, 1394, 1533, 2676, 3839, 3875
RseI CAYNNNNRTG 5 cut(s) 2340, 2424, 2709, 3345, 3870
SapI GCTCTTC 1 cut(s) 700
SatI GCNGC 4 cut(s) 168, 1778, 2715, 3383
Sau3AI GATC 6 cut(s) 231, 1105, 1113, 2596, 3327, 3353
Sau96I GGNCC 6 cut(s) 250, 518, 1261, 2845, 3434, 3769
ScaI AGTACT 1 cut(s) 1534
SchI GAGTC 4 cut(s) 1846, 2885, 3167, 3591
ScrFI CCNGG 7 cut(s) 424, 434, 635, 727, 1561, 3143, 3437
SduI GDGCHC 4 cut(s) 499, 2298, 3308, 3809
SinI GGWCC 3 cut(s) 250, 518, 3769
SmiI ATTTAAAT 1 cut(s) 1309
SmiMI CAYNNNNRTG 5 cut(s) 2340, 2424, 2709, 3345, 3870
SmlI CTYRAG 7 cut(s) 981, 1328, 1942, 2192, 2313, 3050, 3582
SmoI CTYRAG 7 cut(s) 981, 1328, 1942, 2192, 2313, 3050, 3582
SphI GCATGC 2 cut(s) 1281, 1730
SsiI CCGC 5 cut(s) 167, 224, 499, 3351, 3907
SspI AATATT 1 cut(s) 3560
StyD4I CCNGG 7 cut(s) 422, 432, 633, 725, 1559, 3141, 3435
StyI CCWWGG 7 cut(s) 9, 292, 1752, 2265, 2753, 2967, 3780
SwaI ATTTAAAT 1 cut(s) 1309
TaiI ACGT 4 cut(s) 724, 960, 1399, 1764
TaqI TCGA 8 cut(s) 271, 1445, 2146, 2422, 2528, 2821, 2894, 3813
TaqII GACCGA 1 cut(s) 1463
TatI WGTACW 4 cut(s) 39, 1532, 2675, 3874
TauI GCSGC 1 cut(s) 170
TseFI GTSAC 2 cut(s) 1689, 3882
TseI GCWGC 3 cut(s) 1777, 2714, 3382
Tsp45I GTSAC 2 cut(s) 1689, 3882
TspDTI ATGAA 6 cut(s) 492, 689, 1689, 2408, 3407, 3647
TspGWI ACGGA 1 cut(s) 717
Vha464I CTTAAG 1 cut(s) 3050
VpaK11BI GGWCC 3 cut(s) 250, 518, 3769
XapI RAATTY 7 cut(s) 694, 1244, 1794, 2639, 2926, 3146, 3456
XbaI TCTAGA 4 cut(s) 491, 3460, 3490, 3868
XceI RCATGY 4 cut(s) 1281, 1730, 2345, 2566
XmiI GTMKAC 2 cut(s) 718, 3337
XmnI GAANNNNTTC 4 cut(s) 189, 642, 878, 3852
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 5 cut(s) 1306, 1386, 1436, 1728, 3867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.