Rh2DG007300

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003701
Physical Location & Seq
Forward (+)
430819 .. 435177
4359 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG007300.1

Sequence Viewer

Length: 3108 bp
ATGAATCTGGATGATGAAGAACTTAAACAATTGTTCTTGCTATGCGCGACTGTTGTAAGAGAGAACCATATTTGTTTGTCAGATTTGCTGAAATATTGTATGGGTTTAGGTCTGCTTACAAATGTCGATACAGTGGCAGAAGCACAAGATGCGCTTCATAGGCTCGTTGAAAAACTGAAAGATTGGTGTTTGTTGCTGGAAAGTGATGTTGATGGTCTTGTCAAAATGCATGATAATGTATGTCATGTTGCTAACAGGATTGCATACAGAGATCAACACGTCTTGTCATCAGCATATAGAGATGAACCGGAAGATTGGCCGGATAAAGAGTCCTTTAAAAAGTGCACTATGATTTCTTTAAAATCCAACCATATTCCCATGTTTTGCGAAATACCCTGGGAATTCCCAGAACTTCAAATGTTTCATTTGGTTGGTGAAGCTATATCGATTAACTCGGTGGAAATCCCATCCAACTTTTTTAGAGAGATGAAGAAACTCAAAGTGTTGCATTTTACCAACCTGCACATGCTGTCACTACCTCCATCTCTTCAATTCCTGAAGAATCTGCACATGCTCAGTGTAGATAAAAGCATATTGGGAGACGTTGCTTTAGTTGGGCAGCTTAGCAGCTTAGAAATTCTTAGCTTTCGCCAATCCAGAGTTAAACAATTACCTAAAGAAATAGGGCAATTGACTCGTCTACGGTTGTTGGATTTATCTGATTGCTCTGAACTTGAAGTTATTTCACCTGGTGTCATATCAAGCTTGGAGAGACTGGAAGAGTTGAGAATGAGAAACAGCTTTAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTTTGGAGCTGAAACATTTGTCTCAGCTGTCCTCATTAGATATAAATATTTCCAATGCTGACATTCTTCCAGCAGATTTTTTCTCCCAAAAGTTAGAAAGATTCAATATAATTATTGGGGATGCAGAAGGCCTAAGATTTCTGGTGAAAGAAACGGGAGACTTATATGCAGAGGGAGTTAATAATATTGTCCATCTACTAAATGCGGAAGGTTTTGAGCATCTGAAACATGTGCAACTCCACAGCGACGTGGACTTTACTCGCATTAGAAACAGGAAGATTGGTTTCCCTAACTTGACAAGCTTGATGGTTCACCGGTGTGATGGTTCAAGTTTCTTACTGTTATCTTCCGTGGCAAGAAGTCTTGTAAAACTCAAACATCTTGTGATATCTGAATGTCAAATAATGGTAGTAGTATTGACAGAAGAACACGATGAAGAAAACAAGGATAACATGTTTCCTGAGTTAGAAAAGTTGGAGCTAAAGGATCTTCCTCATCTCGTTAGATTCTGCTCAGGAAATTATTTTGAATTTGCATCTATGGAGAGATTGCAATTAGAAAAGTGTGCTAAATTGGAGACATTCATCTGTAATCCCTCGAGTAACAGTATTGCAAGCGAAAGAGAAATGGAAGGAGTGGACACGAATGAGAACCTTGAGATAGTAGTACCATACTTTCTTTTTGATGAGAAGGTAGGGCTACCAAGGTTGGAGAGCTTGACCATCAATGACCTACCTAAGTTCACAAATATTTGGCACAACCAACTTGCTCCAAACTCTTTTTGCAGACTCAGAGATGTTACAGTACAACAATGCAATGGTCTTAAATATATTTTTCAAGACTCCATCATAGCCGGTGTTTTTCAGCAGCTACAAAAGTTGATAGTAAAGGAATGTGGAGTGGAGGAAATAGTTTTTCAGGAAGAGGGCATAGAAAAATTAATGCCTGATCAGTTCTTCCCAAAGCTAACATGTTTGGAATTTACAAGTCTATTCCAACTTGAGAGATTCTATCTAAAGATGCCTGCTATCAACTGGCCGCTAGAAACATTAGTGTTGTCTGATTGTCAGAAAGTGGATATCTTTGCCGCGGAATCTTCAGGCCATCAGAAAATACATGACTTGGGGAATCTGCGGGTTAAACAATATTCTGTTCTATTTGAGAAGAGTTTATTTCCCACACTGGAAGATTTGACCTTCAGCCCCGTGGACATCTGGTACGGTCGACCTGCCCATGAGAAAGCTCTTTTTCCTGCTACCAGTACAGATGGGTCCCTTCCACATTTGAGAACGTTGAGGCTACATGGAATGGAGAAGTTGATGCGTCTCTGGGAGAACACCGAACCTGCAGCTGGCTCAGCTTTTCCACAGTTGAAAACTCTTGAGGTGAAGTTTTCTGGATTGACTAATCTAGAGTTGTCTGCAATATCCTTCCGGAATCTAACAACTATAGAAGTAACCTTTTGCTGGCGATTGCAATATTTGACCACTTATTCAGTAGCTCAAAGTCTCGTGCAACTCAAAACTTTGAAAGTTGACGAATGTAAAAATATGAAAGAAATCTTCACAAGCGAAGGAATGGGAGAAGATGCAAGTAAATGTGAGATTGTTTTCAGGCAGTTGCAAACTTTGGAACTTGGTGATTTAGTAAGTTTGGAACGGTTTTGCTCGAGCAATTGCAGGGTGAAGGTCCCACTCTTGGGAACTTTAAAGGTGAATTGGTGCCCGTTCAAATTGAAGATTTCCTCTGATAAGGTTCTAGAAGTTGATCTGGAGTCTGATATTGAATCACGGTCGCAAAGCATGTCTATGCTTGAACAGGACATCAGACGTTTGCGGGAAGAATCAAGGGCCAAAGAAGGTAACAGCCTCCCGGAGACTCCGGTTGTTCTCGCGCTGCCTGAGACTGGTACTTCTAGCTCTTCTAGCTCGTTGACTGCCAAGAAAACTGCTGATCTTCCTGTGGAGGTGATAGCCCAGCTTAATGCTCTGCTTGGAATTGATCTGGCAGCTCCCTCAGCCTATCAGCAACTCTCAGCACTCCTCCCATTTCTTCAATCAACTAGAAAGGAGTGGGTAGTTGATCAAATAAGAGAAGCTCTTCAGAAATTGGCTCCTGTCGTGGACAAGAAGAGCTCTGAGCTAACTGCCCAACTTGAGGCGAGAGGGTCTGAGGTTATTGCCGGCAGCTCTCAAGTTGAGGCAGAATGTCAACAAGTCATGGGCCGGGAGGCGGAACCGAGATTTGCTTCAGGCCCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1035

Amino Acids

117.39

Weight (kDa)

5.26

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 205 - 302 3.4e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 373 - 476 3.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 515 - 581 1.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 752 - 856 3.4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 528, 2080, 2197
AccB1I GGYRCC 1 cut(s) 2565
AccI GTMKAC 2 cut(s) 700, 2068
AccII CGCG 3 cut(s) 47, 1934, 2738
AccIII TCCGGA 1 cut(s) 2277
AciI CCGC 7 cut(s) 1049, 1883, 1932, 1934, 1978, 2680, 3077
AclI AACGTT 1 cut(s) 2135
AclWI GGATC 1 cut(s) 1338
AcoI YGGCCR 2 cut(s) 317, 1880
AcsI RAATTY 4 cut(s) 401, 636, 1373, 1823
AcuI CTGAAG 5 cut(s) 578, 1926, 2026, 2930, 3078
AdeI CACNNNGTG 1 cut(s) 752
AfaI GTAC 5 cut(s) 1512, 1650, 2063, 2107, 2755
AfiI CCNNNNNNNGG 7 cut(s) 2195, 2310, 2542, 2543, 2750, 3001, 3076
AflIII ACRYGT 4 cut(s) 277, 1072, 1296, 1814
AgeI ACCGGT 1 cut(s) 1158
AjiI CACGTC 2 cut(s) 280, 1093
AjnI CCWGG 3 cut(s) 395, 748, 3100
AleI CACNNNNGTG 1 cut(s) 1161
Alw21I GWGCWC 2 cut(s) 347, 2981
Alw26I GTCTC 9 cut(s) 594, 766, 870, 996, 1415, 2174, 2357, 2714, 2741
Alw44I GTGCAC 1 cut(s) 343
AlwI GGATC 1 cut(s) 1338
Ama87I CYCGRG 2 cut(s) 1441, 2512
Aor13HI TCCGGA 1 cut(s) 2277
AoxI GGCC 7 cut(s) 317, 973, 1880, 1945, 2694, 3067, 3097
ApaLI GTGCAC 1 cut(s) 343
ApeKI GCWGC 7 cut(s) 619, 627, 1711, 2192, 2740, 2852, 3030
ApoI RAATTY 4 cut(s) 401, 636, 1373, 1823
AseI ATTAAT 1 cut(s) 1784
AsiGI ACCGGT 1 cut(s) 1158
Asp700I GAANNNNTTC 1 cut(s) 2943
AspLEI GCGC 3 cut(s) 47, 154, 2740
AspS9I GGNCC 5 cut(s) 2115, 2533, 2694, 3067, 3098
AsuC2I CCSGG 2 cut(s) 2717, 3071
AvaI CYCGRG 2 cut(s) 1441, 2512
AvaII GGWCC 2 cut(s) 2115, 2533
BaeGI GKGCMC 2 cut(s) 347, 2570
BanI GGYRCC 1 cut(s) 2565
BanII GRGCYC 1 cut(s) 2981
BauI CACGAG 1 cut(s) 2354
Bbv12I GWGCWC 2 cut(s) 347, 2981
BbvCI CCTCAGC 1 cut(s) 2860
BbvI GCAGC 7 cut(s) 631, 639, 1723, 2204, 2727, 2864, 3042
BcgI CGANNNNNNTGC 2 cut(s) 677, 711
BciT130I CCWGG 3 cut(s) 397, 750, 3102
BclI TGATCA 2 cut(s) 1792, 2926
BcnI CCSGG 2 cut(s) 2717, 3071
BcoDI GTCTC 9 cut(s) 594, 766, 870, 996, 1415, 2174, 2357, 2714, 2741
BfaI CTAG 6 cut(s) 1886, 2255, 2603, 2760, 2769, 2907
BfmI CTRYAG 2 cut(s) 2190, 2292
BfuAI ACCTGC 3 cut(s) 528, 2080, 2197
BisI GCNGC 9 cut(s) 620, 628, 1712, 1883, 1932, 2193, 2741, 2853, 3031
BlpI GCTNAGC 2 cut(s) 623, 2200
BlsI GCNGC 9 cut(s) 621, 629, 1713, 1884, 1933, 2194, 2742, 2854, 3032
Bme1390I CCNGG 5 cut(s) 397, 750, 2717, 3071, 3102
Bme18I GGWCC 2 cut(s) 2115, 2533
BmeT110I CYCGRG 2 cut(s) 1441, 2512
BmgBI CACGTC 2 cut(s) 280, 1093
BmgT120I GGNCC 5 cut(s) 2115, 2533, 2694, 3067, 3098
BmiI GGNNCC 6 cut(s) 2116, 2117, 2535, 2567, 2958, 3081
BmrFI CCNGG 5 cut(s) 397, 750, 2717, 3071, 3102
BmsI GCATC 7 cut(s) 139, 955, 1072, 1388, 1854, 2154, 2422
BpmI CTGGAG 1 cut(s) 2636
Bpu10I CCTNAGC 2 cut(s) 1357, 2860
Bpu1102I GCTNAGC 2 cut(s) 623, 2200
BpuEI CTTGAG 5 cut(s) 1520, 1865, 2246, 3020, 3021
BpuMI CCSGG 2 cut(s) 2717, 3071
Bsa29I ATCGAT 1 cut(s) 446
BsaJI CCNNGG 7 cut(s) 395, 396, 1194, 1547, 1932, 2049, 3100
BsaWI WCCGGW 4 cut(s) 307, 1158, 2277, 2725
Bsc4I CCNNNNNNNGG 7 cut(s) 2195, 2310, 2542, 2543, 2750, 3001, 3076
Bse118I RCCGGY 3 cut(s) 1158, 1697, 3026
Bse1I ACTGG 5 cut(s) 780, 1883, 2031, 2103, 2755
Bse3DI GCAATG 1 cut(s) 1666
BseAI TCCGGA 1 cut(s) 2277
BseBI CCWGG 3 cut(s) 397, 750, 3102
BseCI ATCGAT 1 cut(s) 446
BseDI CCNNGG 7 cut(s) 395, 396, 1194, 1547, 1932, 2049, 3100
BseGI GGATG 3 cut(s) 16, 467, 970
BseLI CCNNNNNNNGG 7 cut(s) 2195, 2310, 2542, 2543, 2750, 3001, 3076
BseMI GCAATG 1 cut(s) 1666
BseNI ACTGG 5 cut(s) 780, 1883, 2031, 2103, 2755
BseRI GAGGAG 1 cut(s) 2876
BseSI GKGCMC 2 cut(s) 347, 2570
BseXI GCAGC 7 cut(s) 631, 639, 1723, 2204, 2727, 2864, 3042
BseYI CCCAGC 1 cut(s) 2820
BsgI GTGCAG 2 cut(s) 506, 551
Bsh1236I CGCG 3 cut(s) 47, 1934, 2738
Bsh1285I CGRYCG 2 cut(s) 2068, 2639
BshFI GGCC 7 cut(s) 319, 975, 1882, 1947, 2696, 3069, 3099
BshNI GGYRCC 1 cut(s) 2565
BshTI ACCGGT 1 cut(s) 1158
BshVI ATCGAT 1 cut(s) 446
BsiEI CGRYCG 2 cut(s) 2068, 2639
BsiHKAI GWGCWC 2 cut(s) 347, 2981
BsiHKCI CYCGRG 2 cut(s) 1441, 2512
BsiSI CCGG 9 cut(s) 308, 320, 1159, 1698, 2278, 2717, 2726, 3027, 3070
BslFI GGGAC 2 cut(s) 2101, 2519
BslI CCNNNNNNNGG 7 cut(s) 2195, 2310, 2542, 2543, 2750, 3001, 3076
BsmAI GTCTC 9 cut(s) 594, 766, 870, 996, 1415, 2174, 2357, 2714, 2741
BsmBI CGTCTC 2 cut(s) 594, 2174
BsmFI GGGAC 2 cut(s) 2101, 2519
BsnI GGCC 7 cut(s) 319, 975, 1882, 1947, 2696, 3069, 3099
BsoBI CYCGRG 2 cut(s) 1441, 2512
Bsp1286I GDGCHC 3 cut(s) 347, 2570, 2981
Bsp13I TCCGGA 1 cut(s) 2277
Bsp143I GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
Bsp1720I GCTNAGC 2 cut(s) 623, 2200
BspACI CCGC 7 cut(s) 1049, 1883, 1932, 1934, 1978, 2680, 3077
BspANI GGCC 7 cut(s) 319, 975, 1882, 1947, 2696, 3069, 3099
BspDI ATCGAT 1 cut(s) 446
BspEI TCCGGA 1 cut(s) 2277
BspFNI CGCG 3 cut(s) 47, 1934, 2738
BspLI GGNNCC 6 cut(s) 2116, 2117, 2535, 2567, 2958, 3081
BspMAI CTGCAG 1 cut(s) 2194
BspMI ACCTGC 3 cut(s) 528, 2080, 2197
BspPI GGATC 1 cut(s) 1338
BspQI GCTCTTC 3 cut(s) 2770, 2949, 2969
BspT107I GGYRCC 1 cut(s) 2565
BsrDI GCAATG 1 cut(s) 1666
BsrFI RCCGGY 3 cut(s) 1158, 1697, 3026
BsrI ACTGG 5 cut(s) 780, 1883, 2031, 2103, 2755
BssAI RCCGGY 3 cut(s) 1158, 1697, 3026
BssECI CCNNGG 7 cut(s) 395, 396, 1194, 1547, 1932, 2049, 3100
BssMI GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
BssSI CACGAG 1 cut(s) 2354
BssT1I CCWWGG 1 cut(s) 1547
Bst2BI CACGAG 1 cut(s) 2354
Bst2UI CCWGG 3 cut(s) 397, 750, 3102
Bst6I CTCTTC 7 cut(s) 552, 774, 1761, 2003, 2770, 2949, 2969
BstAPI GCANNNNNTGC 1 cut(s) 149
BstC8I GCNNGC 6 cut(s) 841, 1459, 1869, 2197, 2312, 3028
BstDSI CCRYGG 3 cut(s) 1194, 1932, 2049
BstF5I GGATG 3 cut(s) 16, 467, 970
BstFNI CGCG 3 cut(s) 47, 1934, 2738
BstHHI GCGC 3 cut(s) 47, 154, 2740
BstKTI GATC 7 cut(s) 274, 1333, 1795, 2614, 2800, 2848, 2929
BstMAI GTCTC 9 cut(s) 594, 766, 870, 996, 1415, 2174, 2357, 2714, 2741
BstMBI GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
BstMCI CGRYCG 2 cut(s) 2068, 2639
BstMWI GCNNNNNNNGC 5 cut(s) 149, 160, 2201, 2769, 2861
BstNI CCWGG 3 cut(s) 397, 750, 3102
BstNSI RCATGY 6 cut(s) 529, 574, 1076, 1300, 1818, 2650
BstSCI CCNGG 5 cut(s) 395, 748, 2715, 3069, 3100
BstSFI CTRYAG 2 cut(s) 2190, 2292
BstSLI GKGCMC 2 cut(s) 347, 2570
BstUI CGCG 3 cut(s) 47, 1934, 2738
BstV1I GCAGC 7 cut(s) 631, 639, 1723, 2204, 2727, 2864, 3042
BstX2I RGATCY 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 1330
Bsu15I ATCGAT 1 cut(s) 446
BsuRI GGCC 7 cut(s) 319, 975, 1882, 1947, 2696, 3069, 3099
BsuTUI ATCGAT 1 cut(s) 446
BtgI CCRYGG 3 cut(s) 1194, 1932, 2049
BtrI CACGTC 2 cut(s) 280, 1093
BtsCI GGATG 3 cut(s) 16, 467, 970
BtsIMutI CAGTG 3 cut(s) 138, 583, 2024
BveI ACCTGC 3 cut(s) 528, 2080, 2197
Cac8I GCNNGC 6 cut(s) 841, 1459, 1869, 2197, 2312, 3028
CfoI GCGC 3 cut(s) 47, 154, 2740
Cfr10I RCCGGY 3 cut(s) 1158, 1697, 3026
Cfr13I GGNCC 5 cut(s) 2115, 2533, 2694, 3067, 3098
Cfr42I CCGCGG 1 cut(s) 1935
ClaI ATCGAT 1 cut(s) 446
CseI GACGC 1 cut(s) 2156
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 5 cut(s) 1511, 1649, 2062, 2106, 2754
CspAI ACCGGT 1 cut(s) 1158
CviQI GTAC 5 cut(s) 1511, 1649, 2062, 2106, 2754
DpnI GATC 7 cut(s) 273, 1332, 1794, 2613, 2799, 2847, 2928
DpnII GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
DraI TTTAAA 3 cut(s) 337, 360, 2553
DraIII CACNNNGTG 1 cut(s) 752
EaeI YGGCCR 2 cut(s) 317, 1880
Eam1104I CTCTTC 7 cut(s) 552, 774, 1761, 2003, 2770, 2949, 2969
EarI CTCTTC 7 cut(s) 552, 774, 1761, 2003, 2770, 2949, 2969
EciI GGCGGA 1 cut(s) 3092
Ecl136II GAGCTC 1 cut(s) 2979
Eco130I CCWWGG 1 cut(s) 1547
Eco147I AGGCCT 1 cut(s) 975
Eco24I GRGCYC 1 cut(s) 2981
Eco32I GATATC 2 cut(s) 1233, 1924
Eco47I GGWCC 2 cut(s) 2115, 2533
Eco53kI GAGCTC 1 cut(s) 2979
Eco57I CTGAAG 5 cut(s) 578, 1926, 2026, 2930, 3078
Eco88I CYCGRG 2 cut(s) 1441, 2512
EcoICRI GAGCTC 1 cut(s) 2979
EcoO109I RGGNCCY 2 cut(s) 2115, 2533
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 395, 748, 3100
EcoRV GATATC 2 cut(s) 1233, 1924
EcoT14I CCWWGG 1 cut(s) 1547
EcoT22I ATGCAT 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 2981
ErhI CCWWGG 1 cut(s) 1547
Esp3I CGTCTC 2 cut(s) 594, 2174
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FaqI GGGAC 2 cut(s) 2101, 2519
FauI CCCGC 2 cut(s) 1971, 2673
FbaI TGATCA 2 cut(s) 1792, 2926
FblI GTMKAC 2 cut(s) 700, 2068
Fnu4HI GCNGC 9 cut(s) 620, 628, 1712, 1883, 1932, 2193, 2741, 2853, 3031
FokI GGATG 3 cut(s) 23, 454, 977
FriOI GRGCYC 1 cut(s) 2981
Fsp4HI GCNGC 9 cut(s) 620, 628, 1712, 1883, 1932, 2193, 2741, 2853, 3031
FspBI CTAG 6 cut(s) 1886, 2255, 2603, 2760, 2769, 2907
GlaI GCGC 3 cut(s) 46, 153, 2739
GluI GCNGC 9 cut(s) 620, 628, 1712, 1883, 1932, 2193, 2741, 2853, 3031
GsaI CCCAGC 1 cut(s) 2824
GsuI CTGGAG 1 cut(s) 2636
HaeIII GGCC 7 cut(s) 319, 975, 1882, 1947, 2696, 3069, 3099
HapII CCGG 9 cut(s) 308, 320, 1159, 1698, 2278, 2717, 2726, 3027, 3070
HgaI GACGC 1 cut(s) 2156
HhaI GCGC 3 cut(s) 47, 154, 2740
Hin6I GCGC 3 cut(s) 45, 152, 2738
HinP1I GCGC 3 cut(s) 45, 152, 2738
HincII GTYRAC 4 cut(s) 2069, 2380, 2778, 3056
HindII GTYRAC 4 cut(s) 2069, 2380, 2778, 3056
HindIII AAGCTT 2 cut(s) 763, 1144
HpaII CCGG 9 cut(s) 308, 320, 1159, 1698, 2278, 2717, 2726, 3027, 3070
Hpy99I CGWCG 1 cut(s) 1094
HpyCH4IV ACGT 5 cut(s) 279, 603, 1092, 2135, 2674
HpyF10VI GCNNNNNNNGC 5 cut(s) 149, 160, 2201, 2769, 2861
HpySE526I ACGT 5 cut(s) 279, 603, 1092, 2135, 2674
HspAI GCGC 3 cut(s) 45, 152, 2738
KflI GGGWCCC 1 cut(s) 2115
Kpn2I TCCGGA 1 cut(s) 2277
KroI GCCGGC 1 cut(s) 3026
KroNI GCCGGC 1 cut(s) 3028
Ksp22I TGATCA 2 cut(s) 1792, 2926
KspI CCGCGG 1 cut(s) 1935
Kzo9I GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
LguI GCTCTTC 3 cut(s) 2770, 2949, 2969
LmnI GCTCC 5 cut(s) 850, 1321, 1618, 2860, 2962
Lsp1109I GCAGC 7 cut(s) 631, 639, 1723, 2204, 2727, 2864, 3042
LweI GCATC 7 cut(s) 139, 955, 1072, 1388, 1854, 2154, 2422
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 6 cut(s) 1886, 2255, 2603, 2760, 2769, 2907
MaeII ACGT 5 cut(s) 279, 603, 1092, 2135, 2674
MaeIII GTNAC 5 cut(s) 531, 1445, 1642, 2299, 2705
MalI GATC 7 cut(s) 273, 1332, 1794, 2613, 2799, 2847, 2928
MboI GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
MfeI CAATTG 3 cut(s) 29, 689, 2518
MflI RGATCY 1 cut(s) 1330
MhlI GDGCHC 3 cut(s) 347, 2570, 2981
MlyI GAGTC 6 cut(s) 338, 688, 1626, 1679, 2627, 2716
MmeI TCCRAC 6 cut(s) 390, 495, 690, 1299, 1533, 1864
Mph1103I ATGCAT 1 cut(s) 231
MroI TCCGGA 1 cut(s) 2277
MroNI GCCGGC 1 cut(s) 3026
MroXI GAANNNNTTC 1 cut(s) 2943
MslI CAYNNNNRTG 3 cut(s) 234, 1161, 2651
MspA1I CMGCKG 3 cut(s) 871, 1934, 2195
MspI CCGG 9 cut(s) 308, 320, 1159, 1698, 2278, 2717, 2726, 3027, 3070
MspR9I CCNGG 5 cut(s) 397, 750, 2717, 3071, 3102
MunI CAATTG 3 cut(s) 29, 689, 2518
MvaI CCWGG 3 cut(s) 397, 750, 3102
MvnI CGCG 3 cut(s) 47, 1934, 2738
MwoI GCNNNNNNNGC 5 cut(s) 149, 160, 2201, 2769, 2861
NaeI GCCGGC 1 cut(s) 3028
NciI CCSGG 2 cut(s) 2717, 3071
NdeII GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
NgoMIV GCCGGC 1 cut(s) 3026
NlaIV GGNNCC 6 cut(s) 2116, 2117, 2535, 2567, 2958, 3081
NmuCI GTSAC 1 cut(s) 531
NsiI ATGCAT 1 cut(s) 231
NspI RCATGY 6 cut(s) 529, 574, 1076, 1300, 1818, 2650
OliI CACNNNNGTG 1 cut(s) 1161
PaeR7I CTCGAG 2 cut(s) 1441, 2512
PasI CCCWGGG 1 cut(s) 396
PceI AGGCCT 1 cut(s) 975
PciI ACATGT 3 cut(s) 1072, 1296, 1814
PciSI GCTCTTC 3 cut(s) 2770, 2949, 2969
PdiI GCCGGC 1 cut(s) 3028
PdmI GAANNNNTTC 1 cut(s) 2943
PfoI TCCNGGA 1 cut(s) 2715
PinAI ACCGGT 1 cut(s) 1158
PkrI GCNGC 9 cut(s) 621, 629, 1713, 1884, 1933, 2194, 2742, 2854, 3032
PleI GAGTC 6 cut(s) 337, 688, 1626, 1679, 2626, 2716
PpsI GAGTC 6 cut(s) 337, 688, 1626, 1679, 2626, 2716
PpuMI RGGWCCY 2 cut(s) 2115, 2533
PscI ACATGT 3 cut(s) 1072, 1296, 1814
PshBI ATTAAT 1 cut(s) 1784
Psp124BI GAGCTC 1 cut(s) 2981
Psp1406I AACGTT 1 cut(s) 2135
Psp5II RGGWCCY 2 cut(s) 2115, 2533
Psp6I CCWGG 3 cut(s) 395, 748, 3100
PspFI CCCAGC 1 cut(s) 2820
PspGI CCWGG 3 cut(s) 395, 748, 3100
PspN4I GGNNCC 6 cut(s) 2116, 2117, 2535, 2567, 2958, 3081
PspPI GGNCC 5 cut(s) 2115, 2533, 2694, 3067, 3098
PspPPI RGGWCCY 2 cut(s) 2115, 2533
PspXI VCTCGAGB 2 cut(s) 1441, 2512
PstI CTGCAG 1 cut(s) 2194
PsuI RGATCY 1 cut(s) 1330
PvuII CAGCTG 2 cut(s) 871, 2195
RsaI GTAC 5 cut(s) 1512, 1650, 2063, 2107, 2755
RsaNI GTAC 5 cut(s) 1511, 1649, 2062, 2106, 2754
RseI CAYNNNNRTG 3 cut(s) 234, 1161, 2651
SacI GAGCTC 1 cut(s) 2981
SacII CCGCGG 1 cut(s) 1935
SalI GTCGAC 1 cut(s) 2067
SapI GCTCTTC 3 cut(s) 2770, 2949, 2969
SatI GCNGC 9 cut(s) 620, 628, 1712, 1883, 1932, 2193, 2741, 2853, 3031
Sau3AI GATC 7 cut(s) 271, 1330, 1792, 2611, 2797, 2845, 2926
Sau96I GGNCC 5 cut(s) 2115, 2533, 2694, 3067, 3098
SchI GAGTC 6 cut(s) 338, 688, 1626, 1679, 2627, 2716
ScrFI CCNGG 5 cut(s) 397, 750, 2717, 3071, 3102
SduI GDGCHC 3 cut(s) 347, 2570, 2981
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 7 cut(s) 139, 955, 1072, 1388, 1854, 2154, 2422
SfcI CTRYAG 2 cut(s) 2190, 2292
Sfr274I CTCGAG 2 cut(s) 1441, 2512
Sfr303I CCGCGG 1 cut(s) 1935
SgrAI CRCCGGYG 1 cut(s) 1158
SgrBI CCGCGG 1 cut(s) 1935
SinI GGWCC 2 cut(s) 2115, 2533
SlaI CTCGAG 2 cut(s) 1441, 2512
SmiMI CAYNNNNRTG 3 cut(s) 234, 1161, 2651
SmlI CTYRAG 7 cut(s) 1441, 1499, 1844, 2225, 2512, 2999, 3036
SmoI CTYRAG 7 cut(s) 1441, 1499, 1844, 2225, 2512, 2999, 3036
SseBI AGGCCT 1 cut(s) 975
SsiI CCGC 7 cut(s) 1049, 1883, 1932, 1934, 1978, 2680, 3077
SspI AATATT 6 cut(s) 95, 892, 1030, 1594, 1991, 2324
SspMI CTAG 6 cut(s) 1886, 2255, 2603, 2760, 2769, 2907
SstI GAGCTC 1 cut(s) 2981
StuI AGGCCT 1 cut(s) 975
StyD4I CCNGG 5 cut(s) 395, 748, 2715, 3069, 3100
StyI CCWWGG 1 cut(s) 1547
TaiI ACGT 5 cut(s) 282, 606, 1095, 2138, 2677
TaqI TCGA 5 cut(s) 126, 446, 1442, 2068, 2513
TatI WGTACW 2 cut(s) 1648, 2105
TauI GCSGC 2 cut(s) 1885, 1934
TscAI CASTG 3 cut(s) 138, 583, 2031
TseFI GTSAC 1 cut(s) 531
TseI GCWGC 7 cut(s) 619, 627, 1711, 2192, 2740, 2852, 3030
Tsp45I GTSAC 1 cut(s) 531
TspDTI ATGAA 9 cut(s) 17, 30, 146, 318, 413, 503, 1293, 1417, 2411
TspGWI ACGGA 1 cut(s) 1183
TspRI CASTG 3 cut(s) 138, 583, 2031
VneI GTGCAC 1 cut(s) 343
VpaK11BI GGWCC 2 cut(s) 2115, 2533
VspI ATTAAT 1 cut(s) 1784
XapI RAATTY 4 cut(s) 401, 636, 1373, 1823
XbaI TCTAGA 2 cut(s) 2254, 2602
XceI RCATGY 6 cut(s) 529, 574, 1076, 1300, 1818, 2650
XhoI CTCGAG 2 cut(s) 1441, 2512
XmiI GTMKAC 2 cut(s) 700, 2068
XmnI GAANNNNTTC 1 cut(s) 2943
XspI CTAG 6 cut(s) 1886, 2255, 2603, 2760, 2769, 2907
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.