Rw7G017420

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003701
Physical Location & Seq
Reverse (-)
18186123 .. 18187706
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G017420.1

Sequence Viewer

Length: 1401 bp
ATGCTGTCACTACCTTCCTCTCTTCAGTACTTGAAGAATCTGCATGCCCTATTTTTAGATCAATGCATAGTGGGAGACACTGCTTTAGTTGGGGAGCTATGTAACTTGGAAATTCTTAGCTTTTTCCAATCCAATGTCAAACAATTACCAAAGGAAATAGGGAAACTGACCCGTCTTCAGTTGCTGGATTTATCTGATTGCTCTGAACTCCAAGTTATTTCACCTGGTGTCATCTCAAGCTTGGAGAGACTACAAGAATTGAGAATGAGAAACAGCTTCAACAAGTGGGAGGTTGAAGGTGAAAGAAGTAATGCGAGCCTTAGTGAGTTGAAGCATTTGTCTCAGCTATCCGTATTAGAGGTACATATTCCGAATACTGACATCCTTCCAGCAAACTTGTTCTCCCACGAGTTCGAAAGATTCAATATTGTTATTGGAGATGCATGGGTTGACAGATATACATCAGGAGAGAAATTTATGGCTGAGACGACACTCAAGACACTAAAACTCAAACTCAAACTCACTACCAGCATTAAAGAATTGGATGAAGGATTAATATGGCTGCTGGAGAGATGTGAAGACTTGTCCTTAAGTGAGACACAGAATGCTAATAATATTGACCATTATTTAGATACTGAAGATTCTGAGCATCCGCAACATCTGCAAGTCATAAACATCTGTAAGATTGGTTTGCCCAACTTAACAAGATTGGCTGTTCACCAATGTGATGGTTTAAGTTTCTTATTGTCATCTTCCGTGGCAAAAAGTTTTGTACAACTTAAACATCTTCGGATATCTGAATGTCAAATCATGAAGGAGGTAGTATTGTCAGAAGGTCACGGTGAAGAAAAGGTTAACTTGTTTCCGAAGTTAGAACAATTGGAGCTAAATGATCTTCCTTGTCTCACTAGATTCTGTTCAGGAAGTTATGTTGAGTTTGCAGCTCTGAAGAGGTCGCAATTGGAGAAGTGTCCTAAATTGGAGACATTGATCTTTAATCATTTGAGTAACAGAATTCAAATCTACAAAGAAATTAAAGAAAGGGGCACAAATGATGAGAATGTTGAGACAGTTGCACCATACTTTCTTTTAGATGAAAAGGTGAAAACGTTGAGGCTGCATGGAATGGAGATCAAGCTCATGCGTCTCTCAGAAGAGAGCCCAGTTTTTCCACTGTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAACCTAAAGTTGTCTGCAATATCCTTTCGGAATCTAACAATTATAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCAAAGTTTAGTAGGACTGAAAAAATTGAAAGTTGATGAATGTAAAAATATGAAAGAAATCTTCACAAGTGAAAGAATTGAAGAATGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

466

Amino Acids

53.39

Weight (kDa)

5.61

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 30 - 125 3e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 232 - 331 5.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 409 - 451 9e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 653
AclI AACGTT 1 cut(s) 1109
AcsI RAATTY 4 cut(s) 111, 473, 1014, 1182
AcuI CTGAAG 4 cut(s) 8, 161, 657, 968
AdeI CACNNNGTG 1 cut(s) 227
AfaI GTAC 3 cut(s) 29, 363, 774
AflII CTTAAG 1 cut(s) 589
AgsI TTSAA 9 cut(s) 34, 280, 296, 331, 424, 1019, 1210, 1336, 1388
AjnI CCWGG 1 cut(s) 223
AleI CACNNNNGTG 1 cut(s) 723
AluBI AGCT 9 cut(s) 97, 120, 240, 276, 346, 886, 944, 1138, 1307
AluI AGCT 9 cut(s) 97, 120, 240, 276, 346, 886, 944, 1138, 1307
Alw26I GTCTC 9 cut(s) 69, 241, 345, 479, 590, 908, 977, 1061, 1151
AlwNI CAGNNNCTG 1 cut(s) 184
ApeKI GCWGC 3 cut(s) 562, 941, 1117
ApoI RAATTY 4 cut(s) 111, 473, 1014, 1182
ArsI GACNNNNNNTTYG 2 cut(s) 1282, 1314
AseI ATTAAT 1 cut(s) 554
AsuHPI GGTGA 6 cut(s) 213, 311, 710, 854, 1114, 1204
AsuII TTCGAA 1 cut(s) 414
BaeGI GKGCMC 1 cut(s) 1049
BanII GRGCYC 1 cut(s) 1163
BauI CACGAG 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 167, 585
BbvI GCAGC 3 cut(s) 549, 953, 1104
BccI CCATC 1 cut(s) 722
BciT130I CCWGG 1 cut(s) 225
BcoDI GTCTC 9 cut(s) 69, 241, 345, 479, 590, 908, 977, 1061, 1151
BfaI CTAG 2 cut(s) 909, 1187
BfrI CTTAAG 1 cut(s) 589
BisI GCNGC 3 cut(s) 563, 942, 1118
BlsI GCNGC 3 cut(s) 564, 943, 1119
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 225
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 2 cut(s) 430, 658
BmuI ACTGGG 1 cut(s) 1157
BpiI GAAGAC 2 cut(s) 167, 585
BpmI CTGGAG 1 cut(s) 587
Bpu14I TTCGAA 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 220, 479
BsaBI GATNNNNATC 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 756
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bse1I ACTGG 1 cut(s) 1163
Bse8I GATNNNNATC 1 cut(s) 460
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 756
BseGI GGATG 3 cut(s) 381, 550, 649
BseJI GATNNNNATC 1 cut(s) 460
BseMII CTCAG 4 cut(s) 356, 474, 636, 1164
BseNI ACTGG 1 cut(s) 1163
BseSI GKGCMC 1 cut(s) 1049
BseXI GCAGC 3 cut(s) 549, 953, 1104
BsmAI GTCTC 9 cut(s) 69, 241, 345, 479, 590, 908, 977, 1061, 1151
BsmBI CGTCTC 2 cut(s) 479, 1151
BsmI GAATGC 2 cut(s) 610, 1397
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 2 cut(s) 1049, 1163
Bsp1407I TGTACA 1 cut(s) 772
Bsp143I GATC 4 cut(s) 58, 892, 990, 1131
BspACI CCGC 1 cut(s) 653
BspCNI CTCAG 4 cut(s) 355, 475, 637, 1163
BspHI TCATGA 1 cut(s) 810
BspT104I TTCGAA 1 cut(s) 414
BspTI CTTAAG 1 cut(s) 589
BsrGI TGTACA 1 cut(s) 772
BsrI ACTGG 1 cut(s) 1163
BssECI CCNNGG 1 cut(s) 756
BssMI GATC 4 cut(s) 58, 892, 990, 1131
BssSI CACGAG 1 cut(s) 407
Bst2BI CACGAG 1 cut(s) 407
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 842, 1072, 1176
Bst6I CTCTTC 3 cut(s) 27, 944, 1149
BstAFI CTTAAG 1 cut(s) 589
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 1 cut(s) 772
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 45, 316, 1274
BstDEI CTNAG 6 cut(s) 116, 320, 342, 483, 645, 1150
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 3 cut(s) 381, 550, 649
BstKTI GATC 4 cut(s) 61, 895, 993, 1134
BstMAI GTCTC 9 cut(s) 69, 241, 345, 479, 590, 908, 977, 1061, 1151
BstMBI GATC 4 cut(s) 58, 892, 990, 1131
BstMWI GCNNNNNNNGC 1 cut(s) 661
BstNI CCWGG 1 cut(s) 225
BstNSI RCATGY 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 223
BstSLI GKGCMC 1 cut(s) 1049
BstV1I GCAGC 3 cut(s) 549, 953, 1104
BstV2I GAAGAC 2 cut(s) 167, 585
BstXI CCANNNNNNTGG 1 cut(s) 728
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 3 cut(s) 381, 550, 649
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 78, 1172
Cac8I GCNNGC 3 cut(s) 45, 316, 1274
CaiI CAGNNNCTG 1 cut(s) 184
CciI TCATGA 1 cut(s) 810
CseI GACGC 1 cut(s) 1133
CsiI ACCWGGT 1 cut(s) 223
Csp6I GTAC 3 cut(s) 28, 362, 773
CviAII CATG 5 cut(s) 44, 444, 811, 1121, 1141
CviQI GTAC 3 cut(s) 28, 362, 773
DdeI CTNAG 6 cut(s) 116, 320, 342, 483, 645, 1150
DpnI GATC 4 cut(s) 60, 894, 992, 1133
DpnII GATC 4 cut(s) 58, 892, 990, 1131
DraIII CACNNNGTG 1 cut(s) 227
Eam1104I CTCTTC 3 cut(s) 27, 944, 1149
EarI CTCTTC 3 cut(s) 27, 944, 1149
Eco24I GRGCYC 1 cut(s) 1163
Eco32I GATATC 1 cut(s) 795
Eco57I CTGAAG 4 cut(s) 8, 161, 657, 968
EcoRI GAATTC 1 cut(s) 1014
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 795
EcoT22I ATGCAT 2 cut(s) 68, 445
EcoT38I GRGCYC 1 cut(s) 1163
Esp3I CGTCTC 2 cut(s) 479, 1151
FaeI CATG 5 cut(s) 47, 447, 814, 1124, 1144
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FatI CATG 5 cut(s) 43, 443, 810, 1120, 1140
Fnu4HI GCNGC 3 cut(s) 563, 942, 1118
FokI GGATG 3 cut(s) 368, 557, 636
FriOI GRGCYC 1 cut(s) 1163
Fsp4HI GCNGC 3 cut(s) 563, 942, 1118
FspBI CTAG 2 cut(s) 909, 1187
GluI GCNGC 3 cut(s) 563, 942, 1118
GsuI CTGGAG 1 cut(s) 587
HgaI GACGC 1 cut(s) 1133
Hin1II CATG 5 cut(s) 47, 447, 814, 1124, 1144
HincII GTYRAC 2 cut(s) 451, 856
HindII GTYRAC 2 cut(s) 451, 856
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 5 cut(s) 37, 420, 641, 912, 1243
HpaI GTTAAC 1 cut(s) 856
HphI GGTGA 6 cut(s) 213, 311, 710, 854, 1114, 1204
Hpy166II GTNNAC 3 cut(s) 451, 718, 856
Hpy188III TCNNGA 5 cut(s) 465, 496, 811, 921, 1187
Hpy8I GTNNAC 3 cut(s) 451, 718, 856
HpyAV CCTTC 6 cut(s) 24, 290, 395, 542, 808, 827
HpyCH4III ACNGT 3 cut(s) 842, 1072, 1176
HpyCH4IV ACGT 1 cut(s) 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 661
HpyF3I CTNAG 6 cut(s) 116, 320, 342, 483, 645, 1150
HpySE526I ACGT 1 cut(s) 1109
Hsp92II CATG 5 cut(s) 47, 447, 814, 1124, 1144
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 4 cut(s) 58, 892, 990, 1131
LmnI GCTCC 2 cut(s) 94, 883
Lsp1109I GCAGC 3 cut(s) 549, 953, 1104
LweI GCATC 2 cut(s) 430, 658
MabI ACCWGGT 1 cut(s) 223
MaeI CTAG 2 cut(s) 909, 1187
MaeII ACGT 1 cut(s) 1109
MaeIII GTNAC 5 cut(s) 6, 101, 836, 1007, 1261
MalI GATC 4 cut(s) 60, 894, 992, 1133
MboI GATC 4 cut(s) 58, 892, 990, 1131
MfeI CAATTG 2 cut(s) 878, 959
MhlI GDGCHC 2 cut(s) 1049, 1163
MmeI TCCRAC 1 cut(s) 1158
MnlI CCTC 7 cut(s) 28, 283, 352, 811, 945, 1107, 1183
Mph1103I ATGCAT 2 cut(s) 68, 445
MseI TTAA 9 cut(s) 534, 554, 590, 701, 734, 780, 855, 996, 1035
MslI CAYNNNNRTG 1 cut(s) 723
MspCI CTTAAG 1 cut(s) 589
MspR9I CCNGG 1 cut(s) 225
MunI CAATTG 2 cut(s) 878, 959
Mva1269I GAATGC 2 cut(s) 610, 1397
MvaI CCWGG 1 cut(s) 225
MwoI GCNNNNNNNGC 1 cut(s) 661
NdeII GATC 4 cut(s) 58, 892, 990, 1131
NlaIII CATG 5 cut(s) 47, 447, 814, 1124, 1144
NmuCI GTSAC 2 cut(s) 6, 836
NsiI ATGCAT 2 cut(s) 68, 445
NspI RCATGY 1 cut(s) 47
NspV TTCGAA 1 cut(s) 414
OliI CACNNNNGTG 1 cut(s) 723
PaeI GCATGC 1 cut(s) 47
PagI TCATGA 1 cut(s) 810
PctI GAATGC 2 cut(s) 610, 1397
PfeI GAWTC 5 cut(s) 37, 420, 641, 912, 1243
PkrI GCNGC 3 cut(s) 564, 943, 1119
PshBI ATTAAT 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 1109
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PstNI CAGNNNCTG 1 cut(s) 184
RsaI GTAC 3 cut(s) 29, 363, 774
RsaNI GTAC 3 cut(s) 28, 362, 773
RseI CAYNNNNRTG 1 cut(s) 723
SaqAI TTAA 9 cut(s) 534, 554, 590, 701, 734, 780, 855, 996, 1035
SatI GCNGC 3 cut(s) 563, 942, 1118
Sau3AI GATC 4 cut(s) 58, 892, 990, 1131
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 1 cut(s) 225
SduI GDGCHC 2 cut(s) 1049, 1163
SexAI ACCWGGT 1 cut(s) 223
SfaNI GCATC 2 cut(s) 430, 658
SfuI TTCGAA 1 cut(s) 414
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 3 cut(s) 235, 494, 589
SmoI CTYRAG 3 cut(s) 235, 494, 589
SphI GCATGC 1 cut(s) 47
SsiI CCGC 1 cut(s) 653
SspI AATATT 3 cut(s) 427, 616, 1286
SspMI CTAG 2 cut(s) 909, 1187
StyD4I CCNGG 1 cut(s) 223
TaaI ACNGT 3 cut(s) 842, 1072, 1176
TaiI ACGT 1 cut(s) 1112
TaqI TCGA 1 cut(s) 414
TatI WGTACW 2 cut(s) 27, 772
TfiI GAWTC 5 cut(s) 37, 420, 641, 912, 1243
Tru1I TTAA 9 cut(s) 534, 554, 590, 701, 734, 780, 855, 996, 1035
Tru9I TTAA 9 cut(s) 534, 554, 590, 701, 734, 780, 855, 996, 1035
TscAI CASTG 2 cut(s) 85, 1179
TseFI GTSAC 2 cut(s) 6, 836
TseI GCWGC 3 cut(s) 562, 941, 1117
Tsp45I GTSAC 2 cut(s) 6, 836
TspDTI ATGAA 5 cut(s) 561, 827, 1110, 1359, 1373
TspGWI ACGGA 2 cut(s) 340, 745
TspRI CASTG 2 cut(s) 85, 1179
Vha464I CTTAAG 1 cut(s) 589
VspI ATTAAT 1 cut(s) 554
XapI RAATTY 4 cut(s) 111, 473, 1014, 1182
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 1 cut(s) 47
XspI CTAG 2 cut(s) 909, 1187
ZrmI AGTACT 1 cut(s) 29
Zsp2I ATGCAT 2 cut(s) 68, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.