RchiOBHm_Chr7g0236761

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003701
Physical Location & Seq
Forward (+)
61438381 .. 61442513
4133 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21218

Sequence Viewer

Length: 2466 bp
ATGGATTACATGTTTTATAAGCGGGAAGATTTGAAGTTAAATGAATTGCCAAACATCACCAGATTTTGTTCAGGAAGCATAGAAAAATTGGACACAGAAGGTTGGATTAAACTTGGGGAATATAATGCTGCTATGAATAGAAATACGAACGAAATTGAAAAGACTCACTCGGAAGAGAACCTTGATATTACGAGCGAGTTGATTAATATACAATACTTCCTCTTTGACCTAAAGGATGTTTTCCCGAAGTTAACAACCTTGACAGTACATGAGCGTGATCGTCTAAGCTTCTTGTTCACAATTTCTATGGCCAAAAGTCTCGTGGAACTCAAACATCTCGAGATATCCACATGTGAAATCATAGAAGAGATTGTTTCATCAACAAGTGAATACTGTCAAGAAAATATGGAGAACATGTTTCCTAAGCTGGAAGAATTGAAGCTAAATAAACTTCCAAACCTCACCAGATTTTGCTCAGGAAGTTATATTCAACTTTTGTCCTTAGAAAAGTTGGATATACAAGGCTGTACCAAACTGGGCGCATTCATTTCTGATAATATTGCTATGAGTGGTAAAAATACTAGAATCTACAAAGAAATTGAAGAGAAGGACTCCGAAGGGAACAACACGTTGAATACTGTACAATACTTCCTCTATGACGAAAAGATGGGTTTGCCTAACTTGAAAAGCTTGGTGGTTCACCGATGTGATGGTTTACGTTTCTTATCATCCATGGCAAGAAGTCTTGTACAACTCAAACATCTTGAGATATCTGAATTTCAAATCATGGAAGTAGTATTGACCGAAGTATACAGTGAAGAGAACAAGGATAACATGTTTCCTAAGTTAGAACAATTGAAGCTAAATGATCTTCCTTTTCTCGATAGATTCTGCTCTGGAAGTTATGTTGAATTTGCATCTTTGGAGGGATTGCATTTAGAGCAGTGTACTAAATTGGAGACATTTATCTGTAATCCCTTGAGTAACAGTATTGCAAGCTCCAAAGAAGTAGAAGAAAGGAACAGGGATCAGAACATTGAGACAGTTGTACCATGCCTTCTCTTTGACAAAAAGGTAGGGTTCCCAAAGTTAGAGAGATTGACCATCATTGGCCTACCTAAGCTTAAAACAATTTGGCACACCCAACTTGCTCCAGACTCTTTTTGCGGACTCCGGGACGTTACAGTTCAACACTGCAATAGTCTTAAATACATTTTTCCAGGCTCATTGATAGCCGATGTTTTTAATCAGCGGCTACAAAATTTGACAGTGGAGGAATGTGGAGTGGAGGAAATAATTTCCCAGGGAGGGGGACTGGAAGATCAGTTTGTCTTCCCAAACCTAATAAGTTTGGAATTTAAAAGTCTATTCCAACTTAAGAGATTCTATCCAAGGATGCTTGCTTTCAGTTGGCCACTACTCAAGACATTAGTGTTGCTTGATTGTCCGAAAGTGGATATTTTTGCAGCCGAATTTGCAGGCCTTCAGAAAACACATGACTTGCGCAGTTTGGATACACCTGCAGCTAAACTGCTCACTCCGACTAAACAATCTTCTATTCTAATTAACGAGAATTCATTCCCCAAACTGGAAGATTTGACCCTTGTCCCCATGGATATTTGGTACGGTCGACCCATCTCAGCAGTGTGCTGTGAGAAAGCTGTTTTCGCTGGAATCAGTAATAGACACAGAGATGGGTCCCTCCCACATTTAAGAACGTTGAGGCTGCATGGAATGGACATGCGTCTCTGGAAGAAAACCAAACCTGCTGGCCAAAATTCTCCACGGTTGGAAATTCTAGAGGTGAAGTTTTGTAGATTGAATAATCTAGAGTTGTCTTCATTATCCTTCCAGAATCTAACAATTTTAGAAGTAACATTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCGAGGTTTAAGGCAACTAAAAAAACTGAAAGTTGATGAATGTAAAAGTATGAAACAAATATTGGCAAGTGAAGGAATTGGGGAAGATGACAGTAATTGTGAGATTGTTTTCAGCAAGTTGCAACATTTGGAACTCAGTGATTTAACAAGTTTGGAACGCTTTTGCTCAAGAAATTGCACTGTGAAGGTCCCAACCTTGGAAACTGTAGAGGTAAATTGGTGCTCGATCAAGTTGAAGGTTTCTTCTTCTGGCATACTAGTAGTAGATTCCAATATGCATCTGGATGTACAAAAGTCACATTCTAGTAAAGAGGCTTTCAATACCACTGAGTCTACTTGTAGCTCGGAAAAAACTCAGAAACAACCTGTCACAAACTCCTCTGAAGAAGCTGAACATATTGAGTTTAATGCACGGGAACTCCATGATGATAGCCTTATTGAACGTTTGAAGGAAGAATTCAGGGCCAGTAAAGCAGACATCAGACGTTTGGAGGAAGAATTAAGGGCCGAAAAAGCAGAGAGGAGAGATCATAGTGCACTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

821

Amino Acids

94.19

Weight (kDa)

5.56

Isoelectric Point (pI)

46.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 78 - 185 8.5e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 222 - 322 6.9e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 361 - 428 5.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 608 - 715 1.8e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
AarI CACCTGC 1 cut(s) 1528
Acc16I TGCGCA 1 cut(s) 1505
Acc36I ACCTGC 2 cut(s) 1528, 1774
AccI GTMKAC 3 cut(s) 810, 1630, 2252
AciI CCGC 3 cut(s) 22, 1167, 1252
AclI AACGTT 2 cut(s) 1718, 2362
AclWI GGATC 1 cut(s) 1035
AcoI YGGCCR 3 cut(s) 309, 1412, 1771
AcsI RAATTY 9 cut(s) 776, 911, 1261, 1355, 1472, 1573, 1777, 1794, 2375
AcuI CTGAAG 2 cut(s) 1469, 2322
AfaI GTAC 8 cut(s) 267, 529, 642, 750, 949, 1050, 1625, 2208
AfiI CCNNNNNNNGG 4 cut(s) 1308, 1309, 1588, 2116
AflII CTTAAG 1 cut(s) 1376
AflIII ACRYGT 5 cut(s) 9, 350, 414, 627, 834
AhlI ACTAGT 1 cut(s) 2176
AjnI CCWGG 2 cut(s) 1219, 1302
AjuI GAANNNNNNNTTGG 2 cut(s) 1964, 1996
AleI CACNNNNGTG 1 cut(s) 705
Alw21I GWGCWC 2 cut(s) 2144, 2458
Alw26I GTCTC 4 cut(s) 323, 953, 1034, 1751
Alw44I GTGCAC 1 cut(s) 2454
AlwI GGATC 1 cut(s) 1035
Ama87I CYCGRG 2 cut(s) 338, 1920
AoxI GGCC 7 cut(s) 309, 1111, 1412, 1480, 1771, 2382, 2424
ApaLI GTGCAC 1 cut(s) 2454
ApeKI GCWGC 4 cut(s) 128, 1466, 1523, 1726
ApoI RAATTY 9 cut(s) 776, 911, 1261, 1355, 1472, 1573, 1777, 1794, 2375
ArsI GACNNNNNNTTYG 2 cut(s) 1894, 1926
AseI ATTAAT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 1577
AspLEI GCGC 2 cut(s) 542, 1506
AspS9I GGNCC 4 cut(s) 1698, 2107, 2382, 2424
AsuC2I CCSGG 1 cut(s) 1175
AsuHPI GGTGA 4 cut(s) 49, 454, 692, 1816
AvaI CYCGRG 2 cut(s) 338, 1920
AvaII GGWCC 2 cut(s) 1698, 2107
BaeGI GKGCMC 1 cut(s) 2458
BaeI ACNNNNGTAYC 2 cut(s) 1032, 1065
BalI TGGCCA 3 cut(s) 311, 1414, 1773
BarI GAAGNNNNNNTAC 2 cut(s) 1041, 1073
BauI CACGAG 1 cut(s) 320
BbsI GAAGAC 2 cut(s) 1324, 1830
Bbv12I GWGCWC 2 cut(s) 2144, 2458
BbvI GCAGC 4 cut(s) 115, 1478, 1535, 1713
BccI CCATC 5 cut(s) 661, 704, 1112, 1643, 1688
BciT130I CCWGG 2 cut(s) 1221, 1304
BciVI GTATCC 1 cut(s) 1507
BcnI CCSGG 1 cut(s) 1175
BcoDI GTCTC 4 cut(s) 323, 953, 1034, 1751
BcuI ACTAGT 1 cut(s) 2176
BfaI CTAG 5 cut(s) 582, 1799, 1829, 2177, 2223
BfmI CTRYAG 2 cut(s) 1521, 2124
BfrI CTTAAG 1 cut(s) 1376
BfuAI ACCTGC 2 cut(s) 1528, 1774
BfuI GTATCC 1 cut(s) 1507
BisI GCNGC 5 cut(s) 129, 1253, 1467, 1524, 1727
BlsI GCNGC 5 cut(s) 130, 1254, 1468, 1525, 1728
Bme1390I CCNGG 3 cut(s) 1175, 1221, 1304
Bme18I GGWCC 2 cut(s) 1698, 2107
BmeT110I CYCGRG 2 cut(s) 338, 1920
BmgT120I GGNCC 4 cut(s) 1698, 2107, 2382, 2424
BmiI GGNNCC 4 cut(s) 1082, 1699, 1700, 2109
BmrFI CCNGG 3 cut(s) 1175, 1221, 1304
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 3 cut(s) 926, 1386, 2206
BmuI ACTGGG 1 cut(s) 545
BoxI GACNNNNGTC 2 cut(s) 1604, 1743
BpiI GAAGAC 2 cut(s) 1324, 1830
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 1 cut(s) 1137
Bpu10I CCTNAGC 3 cut(s) 423, 475, 1119
BpuEI CTTGAG 4 cut(s) 785, 1000, 1406, 2071
BpuMI CCSGG 1 cut(s) 1175
BsaJI CCNNGG 7 cut(s) 732, 1302, 1303, 1391, 1611, 1784, 2115
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 2322, 2352
Bsc4I CCNNNNNNNGG 4 cut(s) 1308, 1309, 1588, 2116
Bse1I ACTGG 4 cut(s) 540, 1320, 1593, 2385
BseBI CCWGG 2 cut(s) 1221, 1304
BseDI CCNNGG 7 cut(s) 732, 1302, 1303, 1391, 1611, 1784, 2115
BseGI GGATG 4 cut(s) 241, 728, 1401, 2209
BseLI CCNNNNNNNGG 4 cut(s) 1308, 1309, 1588, 2116
BseMII CTCAG 5 cut(s) 489, 1653, 2068, 2238, 2288
BseNI ACTGG 4 cut(s) 540, 1320, 1593, 2385
BseRI GAGGAG 2 cut(s) 2287, 2455
BseSI GKGCMC 1 cut(s) 2458
BseXI GCAGC 4 cut(s) 115, 1478, 1535, 1713
Bsh1285I CGRYCG 1 cut(s) 1630
BshFI GGCC 7 cut(s) 311, 1113, 1414, 1482, 1773, 2384, 2426
BsiEI CGRYCG 1 cut(s) 1630
BsiHKAI GWGCWC 2 cut(s) 2144, 2458
BsiHKCI CYCGRG 2 cut(s) 338, 1920
BsiSI CCGG 1 cut(s) 1174
BslFI GGGAC 5 cut(s) 1190, 1326, 1592, 1684, 2093
BslI CCNNNNNNNGG 4 cut(s) 1308, 1309, 1588, 2116
BsmAI GTCTC 4 cut(s) 323, 953, 1034, 1751
BsmBI CGTCTC 1 cut(s) 1751
BsmFI GGGAC 5 cut(s) 1190, 1326, 1592, 1684, 2093
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 7 cut(s) 311, 1113, 1414, 1482, 1773, 2384, 2426
BsoBI CYCGRG 2 cut(s) 338, 1920
Bsp1286I GDGCHC 2 cut(s) 2144, 2458
Bsp1407I TGTACA 3 cut(s) 640, 748, 2206
Bsp143I GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
Bsp19I CCATGG 2 cut(s) 732, 1611
BspACI CCGC 3 cut(s) 22, 1167, 1252
BspANI GGCC 7 cut(s) 311, 1113, 1414, 1482, 1773, 2384, 2426
BspCNI CTCAG 5 cut(s) 488, 1652, 2067, 2239, 2287
BspLI GGNNCC 4 cut(s) 1082, 1699, 1700, 2109
BspMAI CTGCAG 1 cut(s) 1525
BspMI ACCTGC 2 cut(s) 1528, 1774
BspPI GGATC 1 cut(s) 1035
BspTI CTTAAG 1 cut(s) 1376
BsrGI TGTACA 3 cut(s) 640, 748, 2206
BsrI ACTGG 4 cut(s) 540, 1320, 1593, 2385
BssECI CCNNGG 7 cut(s) 732, 1302, 1303, 1391, 1611, 1784, 2115
BssMI GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
BssNAI GTATAC 1 cut(s) 811
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 4 cut(s) 732, 1391, 1611, 2115
Bst1107I GTATAC 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 2 cut(s) 1221, 1304
Bst6I CTCTTC 4 cut(s) 168, 360, 597, 813
BstAFI CTTAAG 1 cut(s) 1376
BstAUI TGTACA 3 cut(s) 640, 748, 2206
BstC8I GCNNGC 5 cut(s) 997, 1401, 1480, 1771, 1886
BstDSI CCRYGG 3 cut(s) 732, 1611, 1784
BstF5I GGATG 4 cut(s) 241, 728, 1401, 2209
BstHHI GCGC 2 cut(s) 542, 1506
BstKTI GATC 6 cut(s) 280, 871, 1030, 1324, 2148, 2449
BstMAI GTCTC 4 cut(s) 323, 953, 1034, 1751
BstMBI GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
BstMCI CGRYCG 1 cut(s) 1630
BstMWI GCNNNNNNNGC 5 cut(s) 940, 1475, 1667, 2390, 2432
BstNI CCWGG 2 cut(s) 1221, 1304
BstNSI RCATGY 5 cut(s) 13, 354, 418, 838, 1744
BstPAI GACNNNNGTC 2 cut(s) 1604, 1743
BstSCI CCNGG 3 cut(s) 1173, 1219, 1302
BstSFI CTRYAG 2 cut(s) 1521, 2124
BstSLI GKGCMC 1 cut(s) 2458
BstV1I GCAGC 4 cut(s) 115, 1478, 1535, 1713
BstV2I GAAGAC 2 cut(s) 1324, 1830
BstZ17I GTATAC 1 cut(s) 811
BsuI GTATCC 1 cut(s) 1507
BsuRI GGCC 7 cut(s) 311, 1113, 1414, 1482, 1773, 2384, 2426
BtgI CCRYGG 3 cut(s) 732, 1611, 1784
BtsCI GGATG 4 cut(s) 241, 728, 1401, 2209
BtsI GCAGTG 3 cut(s) 950, 1192, 1650
BtsIMutI CAGTG 8 cut(s) 820, 950, 1192, 1275, 1650, 2062, 2097, 2244
BveI ACCTGC 2 cut(s) 1528, 1774
Cac8I GCNNGC 5 cut(s) 997, 1401, 1480, 1771, 1886
CfoI GCGC 2 cut(s) 542, 1506
Cfr13I GGNCC 4 cut(s) 1698, 2107, 2382, 2424
CseI GACGC 1 cut(s) 1733
Csp6I GTAC 8 cut(s) 266, 528, 641, 749, 948, 1049, 1624, 2207
CviQI GTAC 8 cut(s) 266, 528, 641, 749, 948, 1049, 1624, 2207
DpnI GATC 6 cut(s) 279, 870, 1029, 1323, 2147, 2448
DpnII GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
DraI TTTAAA 1 cut(s) 1360
EaeI YGGCCR 3 cut(s) 309, 1412, 1771
Eam1104I CTCTTC 4 cut(s) 168, 360, 597, 813
EarI CTCTTC 4 cut(s) 168, 360, 597, 813
Eco130I CCWWGG 4 cut(s) 732, 1391, 1611, 2115
Eco147I AGGCCT 1 cut(s) 1482
Eco32I GATATC 2 cut(s) 345, 771
Eco47I GGWCC 2 cut(s) 1698, 2107
Eco57I CTGAAG 2 cut(s) 1469, 2322
Eco88I CYCGRG 2 cut(s) 338, 1920
EcoO109I RGGNCCY 2 cut(s) 1698, 2107
EcoRI GAATTC 2 cut(s) 1573, 2375
EcoRII CCWGG 2 cut(s) 1219, 1302
EcoRV GATATC 2 cut(s) 345, 771
EcoT14I CCWWGG 4 cut(s) 732, 1391, 1611, 2115
EcoT22I ATGCAT 1 cut(s) 2199
ErhI CCWWGG 4 cut(s) 732, 1391, 1611, 2115
Esp3I CGTCTC 1 cut(s) 1751
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 5 cut(s) 1190, 1326, 1592, 1684, 2093
FauI CCCGC 1 cut(s) 15
FblI GTMKAC 3 cut(s) 810, 1630, 2252
Fnu4HI GCNGC 5 cut(s) 129, 1253, 1467, 1524, 1727
FokI GGATG 4 cut(s) 248, 715, 1408, 2216
Fsp4HI GCNGC 5 cut(s) 129, 1253, 1467, 1524, 1727
FspBI CTAG 5 cut(s) 582, 1799, 1829, 2177, 2223
FspI TGCGCA 1 cut(s) 1505
GlaI GCGC 2 cut(s) 541, 1505
GluI GCNGC 5 cut(s) 129, 1253, 1467, 1524, 1727
GsuI CTGGAG 1 cut(s) 1137
HaeIII GGCC 7 cut(s) 311, 1113, 1414, 1482, 1773, 2384, 2426
HapII CCGG 1 cut(s) 1174
HgaI GACGC 1 cut(s) 1733
HhaI GCGC 2 cut(s) 542, 1506
Hin6I GCGC 2 cut(s) 540, 1504
HinP1I GCGC 2 cut(s) 540, 1504
HincII GTYRAC 2 cut(s) 252, 1631
HindII GTYRAC 2 cut(s) 252, 1631
HindIII AAGCTT 3 cut(s) 286, 688, 1121
HpaI GTTAAC 1 cut(s) 252
HpaII CCGG 1 cut(s) 1174
HphI GGTGA 4 cut(s) 49, 454, 692, 1816
Hpy166II GTNNAC 9 cut(s) 252, 297, 700, 716, 811, 948, 1631, 2253, 2456
Hpy8I GTNNAC 9 cut(s) 252, 297, 700, 716, 811, 948, 1631, 2253, 2456
HpyCH4IV ACGT 6 cut(s) 629, 718, 1179, 1718, 2362, 2404
HpyF10VI GCNNNNNNNGC 5 cut(s) 940, 1475, 1667, 2390, 2432
HpySE526I ACGT 6 cut(s) 629, 718, 1179, 1718, 2362, 2404
HspAI GCGC 2 cut(s) 540, 1504
KflI GGGWCCC 1 cut(s) 1698
KspAI GTTAAC 1 cut(s) 252
Kzo9I GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
LmnI GCTCC 2 cut(s) 1004, 1156
Lsp1109I GCAGC 4 cut(s) 115, 1478, 1535, 1713
LweI GCATC 3 cut(s) 926, 1386, 2206
MaeI CTAG 5 cut(s) 582, 1799, 1829, 2177, 2223
MaeII ACGT 6 cut(s) 629, 718, 1179, 1718, 2362, 2404
MaeIII GTNAC 5 cut(s) 983, 1180, 1873, 2214, 2287
MalI GATC 6 cut(s) 279, 870, 1029, 1323, 2147, 2448
MboI GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
MfeI CAATTG 1 cut(s) 854
MhlI GDGCHC 2 cut(s) 2144, 2458
MlsI TGGCCA 3 cut(s) 311, 1414, 1773
MluNI TGGCCA 3 cut(s) 311, 1414, 1773
MlyI GAGTC 5 cut(s) 157, 605, 1151, 1164, 2258
MmeI TCCRAC 5 cut(s) 83, 492, 1396, 1565, 1770
Mox20I TGGCCA 3 cut(s) 311, 1414, 1773
Mph1103I ATGCAT 1 cut(s) 2199
MroXI GAANNNNTTC 1 cut(s) 1577
MscI TGGCCA 3 cut(s) 311, 1414, 1773
MslI CAYNNNNRTG 4 cut(s) 273, 705, 1692, 2202
Msp20I TGGCCA 3 cut(s) 311, 1414, 1773
MspA1I CMGCKG 1 cut(s) 1252
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 1 cut(s) 1174
MspR9I CCNGG 3 cut(s) 1175, 1221, 1304
MunI CAATTG 1 cut(s) 854
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 2 cut(s) 1221, 1304
MwoI GCNNNNNNNGC 5 cut(s) 940, 1475, 1667, 2390, 2432
NciI CCSGG 1 cut(s) 1175
NcoI CCATGG 2 cut(s) 732, 1611
NdeII GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
NlaIV GGNNCC 4 cut(s) 1082, 1699, 1700, 2109
NmuCI GTSAC 2 cut(s) 2214, 2287
NsbI TGCGCA 1 cut(s) 1505
NsiI ATGCAT 1 cut(s) 2199
NspI RCATGY 5 cut(s) 13, 354, 418, 838, 1744
OliI CACNNNNGTG 1 cut(s) 705
PaeR7I CTCGAG 2 cut(s) 338, 1920
PaqCI CACCTGC 1 cut(s) 1528
PasI CCCWGGG 1 cut(s) 1303
PceI AGGCCT 1 cut(s) 1482
PciI ACATGT 4 cut(s) 9, 350, 414, 834
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 1577
PfeI GAWTC 6 cut(s) 585, 888, 1383, 1674, 1855, 2186
PfoI TCCNGGA 1 cut(s) 1173
PkrI GCNGC 5 cut(s) 130, 1254, 1468, 1525, 1728
PleI GAGTC 5 cut(s) 157, 605, 1151, 1164, 2257
PpsI GAGTC 5 cut(s) 157, 605, 1151, 1164, 2257
PpuMI RGGWCCY 2 cut(s) 1698, 2107
PscI ACATGT 4 cut(s) 9, 350, 414, 834
PshAI GACNNNNGTC 2 cut(s) 1604, 1743
PshBI ATTAAT 1 cut(s) 204
PsiI TTATAA 1 cut(s) 18
Psp1406I AACGTT 2 cut(s) 1718, 2362
Psp5II RGGWCCY 2 cut(s) 1698, 2107
Psp6I CCWGG 2 cut(s) 1219, 1302
PspGI CCWGG 2 cut(s) 1219, 1302
PspN4I GGNNCC 4 cut(s) 1082, 1699, 1700, 2109
PspPI GGNCC 4 cut(s) 1698, 2107, 2382, 2424
PspPPI RGGWCCY 2 cut(s) 1698, 2107
PspXI VCTCGAGB 1 cut(s) 1920
PstI CTGCAG 1 cut(s) 1525
RsaI GTAC 8 cut(s) 267, 529, 642, 750, 949, 1050, 1625, 2208
RsaNI GTAC 8 cut(s) 266, 528, 641, 749, 948, 1049, 1624, 2207
RseI CAYNNNNRTG 4 cut(s) 273, 705, 1692, 2202
SalI GTCGAC 1 cut(s) 1629
SatI GCNGC 5 cut(s) 129, 1253, 1467, 1524, 1727
Sau3AI GATC 6 cut(s) 277, 868, 1027, 1321, 2145, 2446
Sau96I GGNCC 4 cut(s) 1698, 2107, 2382, 2424
SchI GAGTC 5 cut(s) 157, 605, 1151, 1164, 2258
ScrFI CCNGG 3 cut(s) 1175, 1221, 1304
SduI GDGCHC 2 cut(s) 2144, 2458
SfaNI GCATC 3 cut(s) 926, 1386, 2206
SfcI CTRYAG 2 cut(s) 1521, 2124
Sfr274I CTCGAG 2 cut(s) 338, 1920
SinI GGWCC 2 cut(s) 1698, 2107
SlaI CTCGAG 2 cut(s) 338, 1920
SmiMI CAYNNNNRTG 4 cut(s) 273, 705, 1692, 2202
SmlI CTYRAG 7 cut(s) 338, 764, 979, 1376, 1421, 1920, 2086
SmoI CTYRAG 7 cut(s) 338, 764, 979, 1376, 1421, 1920, 2086
SpeI ACTAGT 1 cut(s) 2176
SseBI AGGCCT 1 cut(s) 1482
SsiI CCGC 3 cut(s) 22, 1167, 1252
SspI AATATT 3 cut(s) 559, 1898, 1980
SspMI CTAG 5 cut(s) 582, 1799, 1829, 2177, 2223
StuI AGGCCT 1 cut(s) 1482
StyD4I CCNGG 3 cut(s) 1173, 1219, 1302
StyI CCWWGG 4 cut(s) 732, 1391, 1611, 2115
TaiI ACGT 6 cut(s) 632, 721, 1182, 1721, 2365, 2407
TaqI TCGA 5 cut(s) 339, 882, 1630, 1921, 2144
TaqII GACCGA 1 cut(s) 818
TatI WGTACW 5 cut(s) 265, 640, 748, 947, 2206
TauI GCSGC 1 cut(s) 1255
TfiI GAWTC 6 cut(s) 585, 888, 1383, 1674, 1855, 2186
TscAI CASTG 8 cut(s) 820, 950, 1199, 1275, 1650, 2062, 2104, 2251
TseFI GTSAC 2 cut(s) 2214, 2287
TseI GCWGC 4 cut(s) 128, 1466, 1523, 1726
Tsp45I GTSAC 2 cut(s) 2214, 2287
TspDTI ATGAA 8 cut(s) 57, 149, 366, 535, 1566, 1830, 1971, 1985
TspRI CASTG 8 cut(s) 820, 950, 1199, 1275, 1650, 2062, 2104, 2251
Vha464I CTTAAG 1 cut(s) 1376
VneI GTGCAC 1 cut(s) 2454
VpaK11BI GGWCC 2 cut(s) 1698, 2107
VspI ATTAAT 1 cut(s) 204
XapI RAATTY 9 cut(s) 776, 911, 1261, 1355, 1472, 1573, 1777, 1794, 2375
XbaI TCTAGA 2 cut(s) 1798, 1828
XceI RCATGY 5 cut(s) 13, 354, 418, 838, 1744
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 2197
XhoI CTCGAG 2 cut(s) 338, 1920
XmiI GTMKAC 3 cut(s) 810, 1630, 2252
XmnI GAANNNNTTC 1 cut(s) 1577
XspI CTAG 5 cut(s) 582, 1799, 1829, 2177, 2223
Zsp2I ATGCAT 1 cut(s) 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.