Rh7BG425400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0003701
Physical Location & Seq
Forward (+)
48497515 .. 48498660
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG425400.1

Sequence Viewer

Length: 1068 bp
ATGGATATTTGGTACGGTCGACCCACCTCAGCAGTGTGCTGTGAGAAAGCTGTTTTCGCTGCGATCAGTAATAGACATACAGAGGCATCCCTCCCACATTTAAGAACGTTGACGCTGCATGGAATGGATATGCGTCTCTGGAAGAAAACCAAACCAGCCGGTCAAAATTCCCCAAAGTTGGAAATTCTAAAGGTGAAGTTATGTAGATTGAATAATCTAGAGTTGTCTTCATTATCCTTCCAGAATCTAACCAATTTGAAAGTAACCTTTTGCTGGCGATTGCAATATTTGACAACTTATTCGGTAGCTCGAGGTTTAAGGCAACTCAAAAAACTGAAAGTTGATGAATGTAAAAGCATGAAACAAATATTGGCAAGTGAAGGAATCGGGGAAGATGACAGTAATTGTGAGATTGTTTTCAGCCAGTTGCAACATTTGGAACTCAGTGATTTAATAAGTTTGGAATGCTTTTGCTCGAGAAATTGCACTGTTAAGGTCCCAGCCTTGGAAACTGTAGAGGTAAATTGGTGCTCGATCAAGTTGAAGGTTTCTTCTGGCATACTAGTTGTTGATTCCAATATGCATCTGGATGTACAAAAGCCACATTCTAGTAAAGAGGCTTTAAATACCACTGAGTCTGCTTGTAGCTCAAAAAAAACTCAGAAACAACCCGTCACAAAATCCTCTGAAGAAGCTGAACATATTGAGTTCAATGCACGGGAACTCCATGATGATAGCCTTACAGATGAGCCGAACAAACACAGGCCTCTATCGCCTGGCATTGCCGAGCGTGAACATGCTGTTGACGGTGTGATTCAAGAAACTTCTATCAACTCGCAAAACATGGAGCATCATGAGGTTATGGGCCTAAAACAGAAGCAAATCGTCACCAATACTTCAAAGAATCTTCCGGAGACCCTCATTGAACGTTTGGAGGAAGAATTAAGGGCCAAAAAAGCAGATTTCAGACGTTTGGAGGAAGAATTATTGCCCAAAAAAGCTGACATCAGACGTTTGGAGGAAGAATTAAGGGCCAAAAAAGCAGACGTCAGCGAGGAGAGACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

355

Amino Acids

40.39

Weight (kDa)

6.87

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 72 - 178 1.9e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1050
AccI GTMKAC 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 910
AclI AACGTT 2 cut(s) 107, 928
AcsI RAATTY 2 cut(s) 166, 183
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 2 cut(s) 14, 594
AfiI CCNNNNNNNGG 3 cut(s) 178, 273, 505
AgsI TTSAA 7 cut(s) 211, 259, 544, 712, 818, 900, 926
AhlI ACTAGT 1 cut(s) 562
AjnI CCWGG 1 cut(s) 775
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 5 cut(s) 50, 308, 648, 695, 1001
AluI AGCT 5 cut(s) 50, 308, 648, 695, 1001
Alw21I GWGCWC 1 cut(s) 533
Alw26I GTCTC 3 cut(s) 140, 908, 1054
Ama87I CYCGRG 2 cut(s) 309, 475
Aor13HI TCCGGA 1 cut(s) 910
AoxI GGCC 4 cut(s) 764, 865, 948, 1032
ApeKI GCWGC 2 cut(s) 59, 115
ApoI RAATTY 2 cut(s) 166, 183
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AspS9I GGNCC 4 cut(s) 496, 865, 948, 1032
AsuHPI GGTGA 2 cut(s) 205, 880
AvaI CYCGRG 2 cut(s) 309, 475
AvaII GGWCC 1 cut(s) 496
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 1 cut(s) 533
BbvCI CCTCAGC 1 cut(s) 28
BbvI GCAGC 2 cut(s) 46, 102
BciT130I CCWGG 1 cut(s) 777
BcoDI GTCTC 3 cut(s) 140, 908, 1054
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 3 cut(s) 218, 563, 609
BfmI CTRYAG 1 cut(s) 513
BisI GCNGC 2 cut(s) 60, 116
BlsI GCNGC 2 cut(s) 61, 117
Bme1390I CCNGG 1 cut(s) 777
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 2 cut(s) 309, 475
BmgT120I GGNCC 4 cut(s) 496, 865, 948, 1032
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 3 cut(s) 95, 592, 859
BpiI GAAGAC 1 cut(s) 219
Bpu10I CCTNAGC 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 1047
BsaI GGTCTC 2 cut(s) 908, 1054
BsaJI CCNNGG 1 cut(s) 504
BsaWI WCCGGW 1 cut(s) 910
BsaXI ACNNNNNCTCC 2 cut(s) 708, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 273, 505
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 424
Bse3DI GCAATG 1 cut(s) 780
BseAI TCCGGA 1 cut(s) 910
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 1 cut(s) 504
BseGI GGATG 2 cut(s) 86, 595
BseLI CCNNNNNNNGG 3 cut(s) 178, 273, 505
BseMI GCAATG 1 cut(s) 780
BseMII CTCAG 4 cut(s) 42, 457, 624, 674
BseNI ACTGG 1 cut(s) 424
BseXI GCAGC 2 cut(s) 46, 102
BseYI CCCAGC 1 cut(s) 499
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 4 cut(s) 766, 867, 950, 1034
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 533
BsiHKCI CYCGRG 2 cut(s) 309, 475
BsiSI CCGG 2 cut(s) 159, 911
BslFI GGGAC 1 cut(s) 482
BslI CCNNNNNNNGG 3 cut(s) 178, 273, 505
BsmAI GTCTC 3 cut(s) 140, 908, 1054
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 482
BsmI GAATGC 1 cut(s) 470
BsnI GGCC 4 cut(s) 766, 867, 950, 1034
Bso31I GGTCTC 2 cut(s) 908, 1054
BsoBI CYCGRG 2 cut(s) 309, 475
Bsp1286I GDGCHC 1 cut(s) 533
Bsp13I TCCGGA 1 cut(s) 910
Bsp1407I TGTACA 1 cut(s) 592
Bsp143I GATC 2 cut(s) 63, 534
BspANI GGCC 4 cut(s) 766, 867, 950, 1034
BspCNI CTCAG 4 cut(s) 41, 456, 625, 673
BspEI TCCGGA 1 cut(s) 910
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 1 cut(s) 498
BspTNI GGTCTC 2 cut(s) 908, 1054
BsrDI GCAATG 1 cut(s) 780
BsrFI RCCGGY 1 cut(s) 158
BsrGI TGTACA 1 cut(s) 592
BsrI ACTGG 1 cut(s) 424
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 504
BssMI GATC 2 cut(s) 63, 534
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 5 cut(s) 17, 401, 490, 514, 809
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 4 cut(s) 28, 443, 633, 660
BstF5I GGATG 2 cut(s) 86, 595
BstKTI GATC 2 cut(s) 66, 537
BstMAI GTCTC 3 cut(s) 140, 908, 1054
BstMBI GATC 2 cut(s) 63, 534
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 4 cut(s) 56, 772, 956, 1040
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 1 cut(s) 775
BstSFI CTRYAG 1 cut(s) 513
BstV1I GCAGC 2 cut(s) 46, 102
BstV2I GAAGAC 1 cut(s) 219
BsuRI GGCC 4 cut(s) 766, 867, 950, 1034
BtsCI GGATG 2 cut(s) 86, 595
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 4 cut(s) 39, 451, 486, 630
Cac8I GCNNGC 1 cut(s) 275
CciI TCATGA 1 cut(s) 853
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 4 cut(s) 496, 865, 948, 1032
CseI GACGC 2 cut(s) 121, 122
Csp6I GTAC 2 cut(s) 13, 593
CviAII CATG 6 cut(s) 119, 358, 728, 797, 844, 854
CviQI GTAC 2 cut(s) 13, 593
DdeI CTNAG 4 cut(s) 28, 443, 633, 660
DpnI GATC 2 cut(s) 65, 536
DpnII GATC 2 cut(s) 63, 534
DraI TTTAAA 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 504
Eco147I AGGCCT 1 cut(s) 766
Eco31I GGTCTC 2 cut(s) 908, 1054
Eco47I GGWCC 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 2 cut(s) 309, 475
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 504
EcoT22I ATGCAT 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 6 cut(s) 122, 361, 731, 800, 847, 857
FaqI GGGAC 1 cut(s) 482
FatI CATG 6 cut(s) 118, 357, 727, 796, 843, 853
FblI GTMKAC 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 60, 116
FokI GGATG 2 cut(s) 73, 602
Fsp4HI GCNGC 2 cut(s) 60, 116
FspBI CTAG 3 cut(s) 218, 563, 609
GluI GCNGC 2 cut(s) 60, 116
GsaI CCCAGC 1 cut(s) 503
HaeIII GGCC 4 cut(s) 766, 867, 950, 1034
HapII CCGG 2 cut(s) 159, 911
HgaI GACGC 2 cut(s) 121, 122
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 6 cut(s) 122, 361, 731, 800, 847, 857
HincII GTYRAC 3 cut(s) 20, 111, 805
HindII GTYRAC 3 cut(s) 20, 111, 805
HinfI GANTC 6 cut(s) 244, 384, 572, 635, 814, 904
HpaII CCGG 2 cut(s) 159, 911
HphI GGTGA 2 cut(s) 205, 880
Hpy166II GTNNAC 4 cut(s) 20, 111, 794, 805
Hpy188I TCNGA 4 cut(s) 663, 688, 968, 1010
Hpy188III TCNNGA 8 cut(s) 139, 218, 241, 477, 587, 818, 854, 911
Hpy8I GTNNAC 4 cut(s) 20, 111, 794, 805
HpyAV CCTTC 3 cut(s) 247, 374, 538
HpyCH4III ACNGT 5 cut(s) 17, 401, 490, 514, 809
HpyCH4IV ACGT 5 cut(s) 107, 928, 970, 1012, 1047
HpyCH4V TGCA 6 cut(s) 118, 283, 430, 486, 583, 716
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 772, 956, 1040
HpyF3I CTNAG 4 cut(s) 28, 443, 633, 660
HpySE526I ACGT 5 cut(s) 107, 928, 970, 1012, 1047
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 6 cut(s) 122, 361, 731, 800, 847, 857
Kpn2I TCCGGA 1 cut(s) 910
Kzo9I GATC 2 cut(s) 63, 534
LmnI GCTCC 1 cut(s) 847
Lsp1109I GCAGC 2 cut(s) 46, 102
LweI GCATC 3 cut(s) 95, 592, 859
MaeI CTAG 3 cut(s) 218, 563, 609
MaeII ACGT 5 cut(s) 107, 928, 970, 1012, 1047
MaeIII GTNAC 3 cut(s) 262, 673, 886
MalI GATC 2 cut(s) 65, 536
MboI GATC 2 cut(s) 63, 534
MboII GAAGA 9 cut(s) 154, 219, 404, 543, 701, 899, 950, 992, 1034
MhlI GDGCHC 1 cut(s) 533
MluCI AATT 9 cut(s) 166, 183, 253, 403, 481, 523, 941, 983, 1025
MlyI GAGTC 1 cut(s) 644
MmeI TCCRAC 1 cut(s) 159
Mph1103I ATGCAT 1 cut(s) 585
MroI TCCGGA 1 cut(s) 910
MseI TTAA 7 cut(s) 101, 317, 452, 492, 623, 944, 1028
MslI CAYNNNNRTG 1 cut(s) 588
MspI CCGG 2 cut(s) 159, 911
MspR9I CCNGG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 470
MvaI CCWGG 1 cut(s) 777
MwoI GCNNNNNNNGC 4 cut(s) 56, 772, 956, 1040
NdeII GATC 2 cut(s) 63, 534
NlaIII CATG 6 cut(s) 122, 361, 731, 800, 847, 857
NlaIV GGNNCC 1 cut(s) 498
NmeAIII GCCGAG 1 cut(s) 811
NmuCI GTSAC 2 cut(s) 673, 886
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 1 cut(s) 800
PaeR7I CTCGAG 2 cut(s) 309, 475
PagI TCATGA 1 cut(s) 853
PceI AGGCCT 1 cut(s) 766
PctI GAATGC 1 cut(s) 470
PfeI GAWTC 5 cut(s) 244, 384, 572, 814, 904
PkrI GCNGC 2 cut(s) 61, 117
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 496
Psp1406I AACGTT 2 cut(s) 107, 928
Psp5II RGGWCCY 1 cut(s) 496
Psp6I CCWGG 1 cut(s) 775
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 775
PspN4I GGNNCC 1 cut(s) 498
PspPI GGNCC 4 cut(s) 496, 865, 948, 1032
PspPPI RGGWCCY 1 cut(s) 496
PspXI VCTCGAGB 1 cut(s) 309
RsaI GTAC 2 cut(s) 14, 594
RsaNI GTAC 2 cut(s) 13, 593
RseI CAYNNNNRTG 1 cut(s) 588
SalI GTCGAC 1 cut(s) 18
SaqAI TTAA 7 cut(s) 101, 317, 452, 492, 623, 944, 1028
SatI GCNGC 2 cut(s) 60, 116
Sau3AI GATC 2 cut(s) 63, 534
Sau96I GGNCC 4 cut(s) 496, 865, 948, 1032
SchI GAGTC 1 cut(s) 644
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 1 cut(s) 533
SfaNI GCATC 3 cut(s) 95, 592, 859
SfcI CTRYAG 1 cut(s) 513
Sfr274I CTCGAG 2 cut(s) 309, 475
SinI GGWCC 1 cut(s) 496
SlaI CTCGAG 2 cut(s) 309, 475
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 309, 475
SmoI CTYRAG 2 cut(s) 309, 475
SpeI ACTAGT 1 cut(s) 562
Sse9I AATT 9 cut(s) 166, 183, 253, 403, 481, 523, 941, 983, 1025
SseBI AGGCCT 1 cut(s) 766
SspI AATATT 2 cut(s) 287, 369
SspMI CTAG 3 cut(s) 218, 563, 609
StuI AGGCCT 1 cut(s) 766
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 5 cut(s) 17, 401, 490, 514, 809
TaiI ACGT 5 cut(s) 110, 931, 973, 1015, 1050
TaqI TCGA 4 cut(s) 19, 310, 476, 533
TasI AATT 9 cut(s) 166, 183, 253, 403, 481, 523, 941, 983, 1025
TatI WGTACW 1 cut(s) 592
TfiI GAWTC 5 cut(s) 244, 384, 572, 814, 904
Tru1I TTAA 7 cut(s) 101, 317, 452, 492, 623, 944, 1028
Tru9I TTAA 7 cut(s) 101, 317, 452, 492, 623, 944, 1028
TscAI CASTG 4 cut(s) 39, 451, 493, 637
TseFI GTSAC 2 cut(s) 673, 886
TseI GCWGC 2 cut(s) 59, 115
Tsp45I GTSAC 2 cut(s) 673, 886
TspDTI ATGAA 3 cut(s) 219, 360, 374
TspRI CASTG 4 cut(s) 39, 451, 493, 637
VpaK11BI GGWCC 1 cut(s) 496
XapI RAATTY 2 cut(s) 166, 183
XbaI TCTAGA 1 cut(s) 217
XceI RCATGY 1 cut(s) 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 583
XhoI CTCGAG 2 cut(s) 309, 475
XmiI GTMKAC 1 cut(s) 19
XspI CTAG 3 cut(s) 218, 563, 609
ZraI GACGTC 1 cut(s) 1048
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.