Rh2BG238600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003701
Physical Location & Seq
Reverse (-)
23785795 .. 23791661
5867 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG238600.1

Sequence Viewer

Length: 4419 bp
ATGGACATTCTTTCAACCATTGTCGAGTTAACAATAATTGAACCCATACACCGGGTGGGACGCCAAGTGGGTTATGTAATTCACTGCAACAGAAATCTTCGCTTCCTAAATTTATCTGTGGAAGACTTGAAAGATCTCACAGACTATTTGGAAACGAAGGTTGATGCGGCTGAAATAATAGGTGAACGAGTTCAACCTGTTTTACAGCATTGGCTGGCAAGAGCAGAGGCGATCACTGTAACGGCAGAGGACCTTCGGATGGATGGAAATCCAGCAAATCTGGAATGTCTCCATGGGTTTTGTCCTAATCTGAAGCACCGTTATCGGCTAGGCAGGAAATCAACAAGATTGACGCAGGAGATTGATGAGCTCGGGCGGATAGGACAAGATCTATTCTTGCAAATGGAGAGGGGTGTATCCACCGGGGAGTACCAGGCTTTTCCATCAAGGACGTCAACTGTGGAGGAAATCATGGATGAACTGAAGAATTCTAGTGCCCGCAGGATCGGGGTGTGCGGACCTAAAGGTGCGGGTAAAACTCTACTTGCCAAAGAAGTTTATAGGCAAGCTAAAGAAGTTTATAGGCAAGCTAGAGAAGATAAAAAGTTATTTGATAATGTGGTTATCCTCCTAGATGTCAAGAAAAATCCAGACCTGGAAGTAATTCAGAGCAAGATTGTTCGTCAGTTAGATATGAAGATTCTTGACGATGAGAATTTAGACGGAAGAGCAAATCGTCTACGTGCCAGGATACTAAACAAGAAGATTCTTGTGATTCTGGATGATGTTTGGGAACAAATTGACTTGGATGCTCTGGGACTTCCTAGTGTCCCTACCTGTAAGATATTGCTGACATCAGAAACACCCTCCGTTGAGGGGTTCCTGCTCGGCCCTTTAAAAGGCGAAGAGGCTTGCTTCCTATTTGAGAAGAGGATAGGTGTTGATGTTAAAGAAGCAGTTGATATACGAAATGTAGCAACTCAAGTTGCTAAAAGATGTGAAGGTTTGCCCATCTTAATTGTCATAGTTGCAAGCACTTTGAAGAGAATATCTACTTTAACTTCATGGGAAAATGCTTTGAGATGCCTTGAAGAGTTTGATACAGATCACGGGATCACACGAAGCCCACACTTGGGGATAAAGTGGAGTTACAATCAATTGAATGATGAGGCAGGGAAGCAGTTGTTCTTGCTATGTGGATTTGCAATATGGGGAAAAGATATTTATTTGCCAGCCTTGATGAAATTTAGTATGGGTTTGGGCCTCTTCAGTAAGGCATACACAATGGAGCAAGCAAAAGATGCATTTAATTCATCGGTTGAAAGACTCAAGGACTTTTATCTACTGACAGACAGTGATGATGATAACAGATATGTCAGAATGCATGACCTTGTACGTGATGTTGCTAACCAGATTGCATTCCGAGATGGACACATCTTATCATCGGCGGAAGATGGGGGTGACTATGAGTTAAAAGAATGGCTAGATAAGGATTTCTTCAAAAAGTGCAGTATGATATCTTCACAATCCACTACAGTTCCCGTGCTTTGTGAAGTAACTAGGATACACCCCAAATTAAAAATGCTTTATTTGCACGGTAAAGGTGATAACTCGGTGGAGATGCCATCCAACTTTTTTAGGGAGATTCAGGAACTCAAAATGTTGGATTTAACCAGACTGCATCTGCTGTCACTACCTTCATCTCTTCAGTTCCTGAAGAATATGCATGCCTTATTTTTAAATCAATGCACCTTGGGAGACGTTGCTTTAGTTGGGCAGCTAAGTAACTTGGAAATTCTCAGCTTTTCTCAGTCCAGTGTCAAACAATTGCCTAAAGAAATAGGGCATTTGACTCGTCTTCGGTTGTTGGATTTATCTGATTGCTCTGAACTTGAAGTTATTTCACCCAATGTCATATCAAGCTTGGAGAGACTAGAAGACTTGAGAATGAGAAACAGCTTTAACAAGTGGGAGGCTGAAGCTAAAAGAAGCAACGCAAGCCTTTCTGAGCTGAAGTATTTGTCTCAGTTATCTGTATTACAAATACATATTCTGAATGTCGACATTCTTCCAGCAAACTTGTTCTCCCACAAGTTAGAAAGATTCAATATTTTTATTGGGGATGCGTGGGTTGAGAGATATACTCGGAAGACACTACTAATGGTTGAGACGACACTGAACACACTAAAACTCAAGCCCACTACCAGCACTGAAGGATTGGATACAGGACTAAAATTGTTGCTGAAGATAACTGAAGACTTGTCTTTGGATGGAACAGAGGGTGTCAATAAGGTGCTCGATCAATTAGATACTCAAGACTTGGAGCATTTGAAACATTTGCAAGTCCAAAACAATGTTGATATAAGATATCTTATTAACAGGGAGGTTCTGTTGCGTAACTTAATAAGCTTCAGTGTTCATCGATGTGATGGTTCAAGTTTCTTATTGTCACCTTCTATGGCAAGAAGTTTTGTACAACTCAAACATCTTGCAATATCAGAATGTCAAATCATGGAAGAGATAGTATCGACAAAAGAATACAGTGAAGAAAACAAGGATAACATGTTTCCTAAGTTGGAGAAATTGGAGCCAAGTGATCTTCCTCATCTCACTAGATTCTGCTCAGGAGGTTATGTTGAATTTGCATCTTTGGAGAGATTGCAACTAGAGAAGTGTACTAAGTTGGAGACTTTCATCTCTAATCATGAGAGTGCTTCACCATATTTTCTTTTTGACGAGAAGGTAGGGTTTCCAAGGCTGGAGATATTGACCGTCAATGACTTACCTAAGCTGACAACAATGTGGCACACCCAACTTGCACGAAACTCTTTTTGCAAACTCAGGGACGTTACAGTTCAACGATGCAATAGTCTTAAAAGCATTTTTCCAGACTCGATAATAGCTGGAGCTTTTCAGGAGCTACAAAATTTGACAGTGGAGGACTGTGTAGTGGAGGAAATAGTTGCCAAGGAAGAGGGACTAGAAAAACAAAACCACGATAAGTTCTTCCCAAAGCTAGAGTGTTTGGAACTTAAAAGTCTATCCCAACTTAAGAGATTCTATCCAAGTATGCCTGCTTTCAGTTGGCCACACCTCAAAACATTAGTGTTGTTTGATTGTCGGCAGGTGGATATTTTTATCCCGGAATTTTCAGACATTGAGAAAACACATGACTTGGGCAGTCAGTCTATTCCAGTTAAACAATCTTCTGTTCTCTTCGATAAGAACTCATTCCCCAACCTAGAAGATTTGACCTTGGTCTGTATGGACAATTTGCACAGCCGACCTGCCCCATCAGTGTTGTGCCCGAAAGCTGTTTTCTCTGGGATCAGTAGTAGACATCCAGATGGCGGATCCCTTCTACATTTAAGAGCTTTTAGGCTGCATGGAATGAAGAAGCCTATGCGTCTCTGGGACACCACCGAATCAGCTGGCCCAGATTTTCCAAATTTGGAAATTCTAGAGGTGAAGTTTTGTGGATTGAATAATCTAGAGTTATCTGCAATATCCTTCCAGAATCTAACAATTTTAGAAGTAACTTTTTGCTGGCGATTGCAATATTTGGAAACTTACACGGTTGCTCAAGGTTTAGTGAGACTCAAAAAATTGAAAGTTGACGAGTGTAAAAATATGAAAGAAATCTTCACAAGAGAAGGAATGGAAGAAGATACAAGTGAATGTAAGATTGTTTTCAGCCGGTTGCAACATTTGGAACTTACTGATTTAATAAGTTTGGAACGGTTTTGCCCGAGAAATTGCAGGGTGAAGGTCCCAGCAACCTTGGGAACTTTAGAGGTAAAATGGTGCTCTTTCGAGCTGAAGATTTCCTCCGATGGGGTACTAGAAGAAATTGTTCCCAATATGCATCTAGATGTACAAAAGTCACATCCCAAAGAAGAGGCCTTGATAACAAATCAGAAACAGCCTTCCCCAATCGCCTTTGAAGGAGCTCAACATGTTGAGTTTAATCCACGAGAACTCCATGATGACAGTCTTAAAACTGAGCCGAACAAAGATGGGCCTCTACGGCCAGGCATTGCTGAAAGTGAACATGAAGTTGAAGCTTTGGTTCAAGAAACTTCTATCAATTCGCAAAACATGAAGCATGCTGAAGCTATGGACCTAAAGCACAAGCGCACTGTCACCAATACTTCACAGGATCTTGAGGAGGCTGATTTTGGCAGACAAGAGAGCCTCACGGTACCTTTGAATGCAAATGTAGGCAACCAGCTTCAGTTGAAACAACTTGATGGTGTAGAAACTGAATCATGGTTGCAAGGCATAGCTATTAAGCTTGATGAAGACATAGGATATTTGCAGAAAGAATTAAGTGCCAAAAAAGCTGAGAGGAGAGATATAGAGCAAAAGCTTGCCGATCTTGTGAGAGCTAAACGATTGTCTGACCCTTTAGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1472

Amino Acids

167.56

Weight (kDa)

5.64

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 151 - 294 1e-16 NB-ARC domain
LRR_14 PF23598 528 - 705 1.2e-08 Leucine-rich repeat region
LRR_RPS2 PF23247 795 - 899 3.2e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 918 - 982 1.1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1163 - 1254 4e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 3115
AatII GACGTC 1 cut(s) 455
Acc36I ACCTGC 2 cut(s) 3115, 3295
Acc65I GGTACC 1 cut(s) 4201
AccB1I GGYRCC 1 cut(s) 4201
AccI GTMKAC 3 cut(s) 739, 2061, 3337
AciI CCGC 7 cut(s) 167, 376, 499, 516, 530, 1448, 3351
AclWI GGATC 6 cut(s) 512, 1121, 3335, 3348, 3361, 4167
AcoI YGGCCR 2 cut(s) 3086, 4028
AcyI GRCGYC 2 cut(s) 61, 452
AdeI CACNNNGTG 1 cut(s) 55
AfaI GTAC 7 cut(s) 431, 1395, 2475, 2677, 3840, 3876, 4203
AfiI CCNNNNNNNGG 8 cut(s) 51, 259, 876, 899, 1132, 1133, 3350, 3834
AflII CTTAAG 1 cut(s) 3050
AflIII ACRYGT 2 cut(s) 2562, 3955
AjnI CCWGG 4 cut(s) 432, 654, 746, 4030
Alw21I GWGCWC 4 cut(s) 372, 2298, 3809, 3952
Alw26I GTCTC 8 cut(s) 293, 1752, 1924, 2028, 2162, 2681, 3413, 3589
AlwI GGATC 6 cut(s) 512, 1121, 3335, 3348, 3361, 4167
AlwNI CAGNNNCTG 1 cut(s) 1714
Ama87I CYCGRG 2 cut(s) 371, 3748
AoxI GGCC 7 cut(s) 889, 1261, 3086, 3433, 3900, 4019, 4028
ApeKI GCWGC 2 cut(s) 1777, 3382
Asp700I GAANNNNTTC 4 cut(s) 189, 663, 3230, 3852
Asp718I GGTACC 1 cut(s) 4201
AspLEI GCGC 1 cut(s) 4137
AspS9I GGNCC 8 cut(s) 250, 518, 890, 1261, 3434, 3769, 4019, 4120
AsuC2I CCSGG 3 cut(s) 53, 424, 3143
AsuHPI GGTGA 9 cut(s) 194, 1472, 1616, 1896, 2442, 2709, 3478, 3775, 4135
AvaI CYCGRG 2 cut(s) 371, 3748
AvaII GGWCC 4 cut(s) 250, 518, 3769, 4120
BaeGI GKGCMC 2 cut(s) 499, 3308
BalI TGGCCA 1 cut(s) 3088
BamHI GGATCC 1 cut(s) 3353
BanI GGYRCC 1 cut(s) 4201
BanII GRGCYC 2 cut(s) 372, 3952
BarI GAAGNNNNNNTAC 2 cut(s) 1504, 1536
BauI CACGAG 1 cut(s) 3972
BbsI GAAGAC 6 cut(s) 129, 1850, 1944, 2156, 2262, 4308
Bbv12I GWGCWC 4 cut(s) 372, 2298, 3809, 3952
BbvI GCAGC 2 cut(s) 1789, 3369
BceAI ACGGC 2 cut(s) 258, 4043
BcgI CGANNNNNNTGC 4 cut(s) 305, 339, 1835, 1869
BciT130I CCWGG 4 cut(s) 434, 656, 748, 4032
BciVI GTATCC 4 cut(s) 427, 744, 1557, 2215
BcnI CCSGG 3 cut(s) 53, 424, 3143
BcoDI GTCTC 8 cut(s) 293, 1752, 1924, 2028, 2162, 2681, 3413, 3589
BfmI CTRYAG 1 cut(s) 1533
BfrI CTTAAG 1 cut(s) 3050
BfuAI ACCTGC 2 cut(s) 3115, 3295
BfuI GTATCC 4 cut(s) 427, 744, 1557, 2215
BglI GCCNNNNNGGC 1 cut(s) 4027
BglII AGATCT 2 cut(s) 133, 388
BisI GCNGC 3 cut(s) 168, 1778, 3383
BlsI GCNGC 3 cut(s) 169, 1779, 3384
Bme1390I CCNGG 7 cut(s) 53, 424, 434, 656, 748, 3143, 4032
Bme18I GGWCC 4 cut(s) 250, 518, 3769, 4120
BmeT110I CYCGRG 2 cut(s) 371, 3748
BmgT120I GGNCC 8 cut(s) 250, 518, 890, 1261, 3434, 3769, 4019, 4120
BmiI GGNNCC 5 cut(s) 881, 2589, 3355, 3771, 4203
BmrFI CCNGG 7 cut(s) 53, 424, 434, 656, 748, 3143, 4032
BoxI GACNNNNGTC 1 cut(s) 3257
BpiI GAAGAC 6 cut(s) 129, 1850, 1944, 2156, 2262, 4308
BplI GAGNNNNNCTC 2 cut(s) 2128, 2160
BpmI CTGGAG 2 cut(s) 2780, 2925
Bpu10I CCTNAGC 2 cut(s) 2623, 2787
BpuEI CTTGAG 7 cut(s) 966, 1313, 1963, 2177, 2298, 3567, 4184
BpuMI CCSGG 3 cut(s) 53, 424, 3143
Bsa29I ATCGAT 1 cut(s) 2422
BsaAI YACGTR 2 cut(s) 743, 1397
BsaBI GATNNNNATC 2 cut(s) 267, 1104
BsaHI GRCGYC 2 cut(s) 61, 452
BsaJI CCNNGG 7 cut(s) 292, 423, 1752, 2753, 2967, 3255, 3780
BsaXI ACNNNNNCTCC 8 cut(s) 2069, 2099, 2375, 2405, 2619, 2649, 3963, 3993
Bsc4I CCNNNNNNNGG 8 cut(s) 51, 259, 876, 899, 1132, 1133, 3350, 3834
Bse118I RCCGGY 1 cut(s) 3696
Bse1I ACTGG 2 cut(s) 1815, 3194
Bse3DI GCAATG 1 cut(s) 4035
Bse8I GATNNNNATC 2 cut(s) 267, 1104
BseBI CCWGG 4 cut(s) 434, 656, 748, 4032
BseCI ATCGAT 1 cut(s) 2422
BseDI CCNNGG 7 cut(s) 292, 423, 1752, 2753, 2967, 3255, 3780
BseJI GATNNNNATC 2 cut(s) 267, 1104
BseLI CCNNNNNNNGG 8 cut(s) 51, 259, 876, 899, 1132, 1133, 3350, 3834
BseMI GCAATG 1 cut(s) 4035
BseMII CTCAG 8 cut(s) 1813, 1823, 1998, 2039, 2637, 2854, 3993, 4335
BseNI ACTGG 2 cut(s) 1815, 3194
BseRI GAGGAG 2 cut(s) 4181, 4363
BseSI GKGCMC 2 cut(s) 499, 3308
BseXI GCAGC 2 cut(s) 1789, 3369
BseYI CCCAGC 1 cut(s) 3772
BsgI GTGCAG 1 cut(s) 1528
BshFI GGCC 7 cut(s) 891, 1263, 3088, 3435, 3902, 4021, 4030
BshNI GGYRCC 1 cut(s) 4201
BshVI ATCGAT 1 cut(s) 2422
BsiHKAI GWGCWC 4 cut(s) 372, 2298, 3809, 3952
BsiHKCI CYCGRG 2 cut(s) 371, 3748
BsiSI CCGG 4 cut(s) 52, 423, 3143, 3697
BslFI GGGAC 7 cut(s) 72, 815, 831, 2858, 2991, 3428, 3755
BslI CCNNNNNNNGG 8 cut(s) 51, 259, 876, 899, 1132, 1133, 3350, 3834
BsmAI GTCTC 8 cut(s) 293, 1752, 1924, 2028, 2162, 2681, 3413, 3589
BsmBI CGTCTC 3 cut(s) 1752, 2162, 3413
BsmFI GGGAC 7 cut(s) 72, 815, 831, 2858, 2991, 3428, 3755
BsmI GAATGC 3 cut(s) 1386, 1418, 4216
BsnI GGCC 7 cut(s) 891, 1263, 3088, 3435, 3902, 4021, 4030
BsoBI CYCGRG 2 cut(s) 371, 3748
Bsp1286I GDGCHC 6 cut(s) 372, 499, 2298, 3308, 3809, 3952
Bsp1407I TGTACA 2 cut(s) 2473, 3874
Bsp19I CCATGG 1 cut(s) 292
BspACI CCGC 7 cut(s) 167, 376, 499, 516, 530, 1448, 3351
BspANI GGCC 7 cut(s) 891, 1263, 3088, 3435, 3902, 4021, 4030
BspCNI CTCAG 8 cut(s) 1812, 1822, 1999, 2038, 2636, 2853, 3994, 4336
BspDI ATCGAT 1 cut(s) 2422
BspHI TCATGA 1 cut(s) 2704
BspLI GGNNCC 5 cut(s) 881, 2589, 3355, 3771, 4203
BspMI ACCTGC 2 cut(s) 3115, 3295
BspPI GGATC 6 cut(s) 512, 1121, 3335, 3348, 3361, 4167
BspQI GCTCTTC 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 4201
BspTI CTTAAG 1 cut(s) 3050
BsrDI GCAATG 1 cut(s) 4035
BsrFI RCCGGY 1 cut(s) 3696
BsrGI TGTACA 2 cut(s) 2473, 3874
BsrI ACTGG 2 cut(s) 1815, 3194
BssAI RCCGGY 1 cut(s) 3696
BssECI CCNNGG 7 cut(s) 292, 423, 1752, 2753, 2967, 3255, 3780
BssNI GRCGYC 2 cut(s) 61, 452
BssSI CACGAG 1 cut(s) 3972
BssT1I CCWWGG 6 cut(s) 292, 1752, 2753, 2967, 3255, 3780
Bst2BI CACGAG 1 cut(s) 3972
Bst2UI CCWGG 4 cut(s) 434, 656, 748, 4032
BstACI GRCGYC 2 cut(s) 61, 452
BstAFI CTTAAG 1 cut(s) 3050
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstAUI TGTACA 2 cut(s) 2473, 3874
BstBAI YACGTR 2 cut(s) 743, 1397
BstDSI CCRYGG 1 cut(s) 292
BstENI CCTNNNNNAGG 1 cut(s) 897
BstHHI GCGC 1 cut(s) 4137
BstMAI GTCTC 8 cut(s) 293, 1752, 1924, 2028, 2162, 2681, 3413, 3589
BstMWI GCNNNNNNNGC 5 cut(s) 1301, 1591, 1998, 4027, 4340
BstNI CCWGG 4 cut(s) 434, 656, 748, 4032
BstNSI RCATGY 4 cut(s) 1730, 2566, 3959, 4109
BstPAI GACNNNNGTC 1 cut(s) 3257
BstSCI CCNGG 7 cut(s) 51, 422, 432, 654, 746, 3141, 4030
BstSFI CTRYAG 1 cut(s) 1533
BstSLI GKGCMC 2 cut(s) 499, 3308
BstV1I GCAGC 2 cut(s) 1789, 3369
BstV2I GAAGAC 6 cut(s) 129, 1850, 1944, 2156, 2262, 4308
BstX2I RGATCY 4 cut(s) 133, 388, 3353, 4159
BstYI RGATCY 4 cut(s) 133, 388, 3353, 4159
Bsu15I ATCGAT 1 cut(s) 2422
BsuI GTATCC 4 cut(s) 427, 744, 1557, 2215
BsuRI GGCC 7 cut(s) 891, 1263, 3088, 3435, 3902, 4021, 4030
BsuTUI ATCGAT 1 cut(s) 2422
BtgI CCRYGG 1 cut(s) 292
BtsI GCAGTG 1 cut(s) 82
BveI ACCTGC 2 cut(s) 3115, 3295
CaiI CAGNNNCTG 1 cut(s) 1714
CciI TCATGA 1 cut(s) 2704
CfoI GCGC 1 cut(s) 4137
Cfr10I RCCGGY 1 cut(s) 3696
Cfr13I GGNCC 8 cut(s) 250, 518, 890, 1261, 3434, 3769, 4019, 4120
ClaI ATCGAT 1 cut(s) 2422
CseI GACGC 3 cut(s) 69, 361, 3395
Csp6I GTAC 7 cut(s) 430, 1394, 2474, 2676, 3839, 3875, 4202
CviQI GTAC 7 cut(s) 430, 1394, 2474, 2676, 3839, 3875, 4202
DraI TTTAAA 2 cut(s) 897, 1740
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 2 cut(s) 3086, 4028
EciI GGCGGA 3 cut(s) 391, 1463, 3366
Ecl136II GAGCTC 2 cut(s) 370, 3950
Eco130I CCWWGG 6 cut(s) 292, 1752, 2753, 2967, 3255, 3780
Eco147I AGGCCT 1 cut(s) 3902
Eco24I GRGCYC 2 cut(s) 372, 3952
Eco32I GATATC 2 cut(s) 1518, 2369
Eco47I GGWCC 4 cut(s) 250, 518, 3769, 4120
Eco53kI GAGCTC 2 cut(s) 370, 3950
Eco88I CYCGRG 2 cut(s) 371, 3748
EcoICRI GAGCTC 2 cut(s) 370, 3950
EcoNI CCTNNNNNAGG 1 cut(s) 897
EcoO109I RGGNCCY 2 cut(s) 250, 3769
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 4 cut(s) 432, 654, 746, 4030
EcoRV GATATC 2 cut(s) 1518, 2369
EcoT14I CCWWGG 6 cut(s) 292, 1752, 2753, 2967, 3255, 3780
EcoT22I ATGCAT 4 cut(s) 1306, 1386, 1728, 3867
EcoT38I GRGCYC 2 cut(s) 372, 3952
ErhI CCWWGG 6 cut(s) 292, 1752, 2753, 2967, 3255, 3780
Esp3I CGTCTC 3 cut(s) 1752, 2162, 3413
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 7 cut(s) 72, 815, 831, 2858, 2991, 3428, 3755
FauI CCCGC 2 cut(s) 506, 523
FblI GTMKAC 3 cut(s) 739, 2061, 3337
Fnu4HI GCNGC 3 cut(s) 168, 1778, 3383
FriOI GRGCYC 2 cut(s) 372, 3952
Fsp4HI GCNGC 3 cut(s) 168, 1778, 3383
GlaI GCGC 1 cut(s) 4136
GluI GCNGC 3 cut(s) 168, 1778, 3383
GsaI CCCAGC 1 cut(s) 3776
GsuI CTGGAG 2 cut(s) 2780, 2925
HaeIII GGCC 7 cut(s) 891, 1263, 3088, 3435, 3902, 4021, 4030
HapII CCGG 4 cut(s) 52, 423, 3143, 3697
HgaI GACGC 3 cut(s) 69, 361, 3395
HhaI GCGC 1 cut(s) 4137
Hin1I GRCGYC 2 cut(s) 61, 452
Hin6I GCGC 1 cut(s) 4135
HinP1I GCGC 1 cut(s) 4135
HincII GTYRAC 4 cut(s) 30, 456, 2062, 3616
HindII GTYRAC 4 cut(s) 30, 456, 2062, 3616
HindIII AAGCTT 5 cut(s) 1921, 2407, 4062, 4292, 4367
HpaI GTTAAC 1 cut(s) 30
HpaII CCGG 4 cut(s) 52, 423, 3143, 3697
HphI GGTGA 9 cut(s) 194, 1472, 1616, 1896, 2442, 2709, 3478, 3775, 4135
Hpy166II GTNNAC 9 cut(s) 30, 185, 456, 740, 2062, 2676, 3338, 3616, 4049
Hpy8I GTNNAC 9 cut(s) 30, 185, 456, 740, 2062, 2676, 3338, 3616, 4049
HpyCH4IV ACGT 5 cut(s) 452, 742, 1396, 1761, 2847
HpyF10VI GCNNNNNNNGC 5 cut(s) 1301, 1591, 1998, 4027, 4340
HpySE526I ACGT 5 cut(s) 452, 742, 1396, 1761, 2847
Hsp92I GRCGYC 2 cut(s) 61, 452
HspAI GCGC 1 cut(s) 4135
KpnI GGTACC 1 cut(s) 4205
KspAI GTTAAC 1 cut(s) 30
LguI GCTCTTC 1 cut(s) 721
LmnI GCTCC 6 cut(s) 1288, 2323, 2587, 2906, 2917, 3947
Lsp1109I GCAGC 2 cut(s) 1789, 3369
MaeII ACGT 5 cut(s) 452, 742, 1396, 1761, 2847
MfeI CAATTG 2 cut(s) 1157, 1826
MflI RGATCY 4 cut(s) 133, 388, 3353, 4159
MhlI GDGCHC 6 cut(s) 372, 499, 2298, 3308, 3809, 3952
MlsI TGGCCA 1 cut(s) 3088
MluNI TGGCCA 1 cut(s) 3088
MlyI GAGTC 4 cut(s) 1320, 1846, 2885, 3591
MmeI TCCRAC 5 cut(s) 1644, 1653, 1848, 2556, 2664
Mox20I TGGCCA 1 cut(s) 3088
Mph1103I ATGCAT 4 cut(s) 1306, 1386, 1728, 3867
MroXI GAANNNNTTC 4 cut(s) 189, 663, 3230, 3852
MscI TGGCCA 1 cut(s) 3088
MslI CAYNNNNRTG 4 cut(s) 2424, 2709, 3345, 3870
Msp20I TGGCCA 1 cut(s) 3088
MspA1I CMGCKG 1 cut(s) 3431
MspCI CTTAAG 1 cut(s) 3050
MspI CCGG 4 cut(s) 52, 423, 3143, 3697
MspR9I CCNGG 7 cut(s) 53, 424, 434, 656, 748, 3143, 4032
MunI CAATTG 2 cut(s) 1157, 1826
Mva1269I GAATGC 3 cut(s) 1386, 1418, 4216
MvaI CCWGG 4 cut(s) 434, 656, 748, 4032
MwoI GCNNNNNNNGC 5 cut(s) 1301, 1591, 1998, 4027, 4340
NciI CCSGG 3 cut(s) 53, 424, 3143
NcoI CCATGG 1 cut(s) 292
NlaIV GGNNCC 5 cut(s) 881, 2589, 3355, 3771, 4203
NmeAIII GCCGAG 1 cut(s) 867
NmuCI GTSAC 5 cut(s) 1460, 1689, 2448, 3882, 4141
NsiI ATGCAT 4 cut(s) 1306, 1386, 1728, 3867
NspI RCATGY 4 cut(s) 1730, 2566, 3959, 4109
PaeI GCATGC 2 cut(s) 1730, 4109
PagI TCATGA 1 cut(s) 2704
PaqCI CACCTGC 1 cut(s) 3115
PceI AGGCCT 1 cut(s) 3902
PciI ACATGT 2 cut(s) 2562, 3955
PciSI GCTCTTC 1 cut(s) 721
PctI GAATGC 3 cut(s) 1386, 1418, 4216
PdmI GAANNNNTTC 4 cut(s) 189, 663, 3230, 3852
PfoI TCCNGGA 1 cut(s) 3141
PkrI GCNGC 3 cut(s) 169, 1779, 3384
PleI GAGTC 4 cut(s) 1320, 1846, 2885, 3591
PpsI GAGTC 4 cut(s) 1320, 1846, 2885, 3591
Ppu21I YACGTR 2 cut(s) 743, 1397
PpuMI RGGWCCY 2 cut(s) 250, 3769
PscI ACATGT 2 cut(s) 2562, 3955
PshAI GACNNNNGTC 1 cut(s) 3257
Psp124BI GAGCTC 2 cut(s) 372, 3952
Psp5II RGGWCCY 2 cut(s) 250, 3769
Psp6I CCWGG 4 cut(s) 432, 654, 746, 4030
PspFI CCCAGC 1 cut(s) 3772
PspGI CCWGG 4 cut(s) 432, 654, 746, 4030
PspN4I GGNNCC 5 cut(s) 881, 2589, 3355, 3771, 4203
PspPI GGNCC 8 cut(s) 250, 518, 890, 1261, 3434, 3769, 4019, 4120
PspPPI RGGWCCY 2 cut(s) 250, 3769
PstNI CAGNNNCTG 1 cut(s) 1714
PsuI RGATCY 4 cut(s) 133, 388, 3353, 4159
PvuII CAGCTG 1 cut(s) 3431
RsaI GTAC 7 cut(s) 431, 1395, 2475, 2677, 3840, 3876, 4203
RsaNI GTAC 7 cut(s) 430, 1394, 2474, 2676, 3839, 3875, 4202
RseI CAYNNNNRTG 4 cut(s) 2424, 2709, 3345, 3870
SacI GAGCTC 2 cut(s) 372, 3952
SalI GTCGAC 1 cut(s) 2060
SapI GCTCTTC 1 cut(s) 721
SatI GCNGC 3 cut(s) 168, 1778, 3383
Sau96I GGNCC 8 cut(s) 250, 518, 890, 1261, 3434, 3769, 4019, 4120
SchI GAGTC 4 cut(s) 1320, 1846, 2885, 3591
ScrFI CCNGG 7 cut(s) 53, 424, 434, 656, 748, 3143, 4032
SduI GDGCHC 6 cut(s) 372, 499, 2298, 3308, 3809, 3952
SfcI CTRYAG 1 cut(s) 1533
SfiI GGCCNNNNNGGCC 1 cut(s) 4027
SinI GGWCC 4 cut(s) 250, 518, 3769, 4120
SmiMI CAYNNNNRTG 4 cut(s) 2424, 2709, 3345, 3870
SmlI CTYRAG 8 cut(s) 981, 1328, 1942, 2192, 2313, 3050, 3582, 4163
SmoI CTYRAG 8 cut(s) 981, 1328, 1942, 2192, 2313, 3050, 3582, 4163
SphI GCATGC 2 cut(s) 1730, 4109
SseBI AGGCCT 1 cut(s) 3902
SsiI CCGC 7 cut(s) 167, 376, 499, 516, 530, 1448, 3351
SspI AATATT 2 cut(s) 2110, 3560
SstI GAGCTC 2 cut(s) 372, 3952
StuI AGGCCT 1 cut(s) 3902
StyD4I CCNGG 7 cut(s) 51, 422, 432, 654, 746, 3141, 4030
StyI CCWWGG 6 cut(s) 292, 1752, 2753, 2967, 3255, 3780
TaiI ACGT 5 cut(s) 455, 745, 1399, 1764, 2850
TaqI TCGA 8 cut(s) 24, 2061, 2298, 2422, 2528, 2894, 3219, 3813
TatI WGTACW 3 cut(s) 2473, 2675, 3874
TauI GCSGC 1 cut(s) 170
TseFI GTSAC 5 cut(s) 1460, 1689, 2448, 3882, 4141
TseI GCWGC 2 cut(s) 1777, 3382
Tsp45I GTSAC 5 cut(s) 1460, 1689, 2448, 3882, 4141
TspGWI ACGGA 2 cut(s) 738, 859
Vha464I CTTAAG 1 cut(s) 3050
VpaK11BI GGWCC 4 cut(s) 250, 518, 3769, 4120
XagI CCTNNNNNAGG 1 cut(s) 897
XbaI TCTAGA 3 cut(s) 3460, 3490, 3868
XceI RCATGY 4 cut(s) 1730, 2566, 3959, 4109
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmiI GTMKAC 3 cut(s) 739, 2061, 3337
XmnI GAANNNNTTC 4 cut(s) 189, 663, 3230, 3852
ZraI GACGTC 1 cut(s) 453
Zsp2I ATGCAT 4 cut(s) 1306, 1386, 1728, 3867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.