RchiOBHm_Chr2g0100661

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004758
Physical Location & Seq
Forward (+)
12549811 .. 12550395
585 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47529

Sequence Viewer

Length: 543 bp
ATGAATGAATTGTCGCCGAACCCAAAAAGCCAATTTTTTATACTCATATCAAACCTCTCATCTTCTCTGAAAACCCCATCTTCATCATCCACCACGATCTCAGAAACCCACCTTTCAAATTCAACTACTGATCATCAACCTCAAGCCCCAAAATGCCTATCACTTGAAGTACCCTTCATTCCCACATGCACAAATACCCCACCTTCCTTAGCCAACGCCCTCATCCACTACGCCACCACAAACATCACCCCACAACAAACCTTCAAAGATATATTAGTATCAGCAAGGATCCTAGCAAAGAGGACGCCCTGCAACTTCCTCGTCTTCGGCTTAGGCCGGGATAGCCTCATGTGGGCGGCTCTCAACTATGGTGGCTCCACCGTTTTCCTAAAAGGAAGACTGAGATTGGAAGGAAGTGTACCTGCGACGACGAGCTTCAATGACAAAATTAAGCGGCGCAACGGGAGACCCATCATGAATCATATGAGTGAAGTGAAGACTATGGTTTTCAGATTCTGCTGGTATGCATGTAAAGGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

180

Amino Acids

19.9

Weight (kDa)

9.75

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 90 - 131 1.7e-14 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 430
AciI CCGC 2 cut(s) 356, 454
AclWI GGATC 2 cut(s) 283, 296
AcsI RAATTY 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 2 cut(s) 171, 420
AfiI CCNNNNNNNGG 1 cut(s) 352
AgsI TTSAA 5 cut(s) 117, 123, 167, 265, 439
AluBI AGCT 1 cut(s) 435
AluI AGCT 1 cut(s) 435
Alw26I GTCTC 1 cut(s) 460
AlwI GGATC 2 cut(s) 283, 296
AlwNI CAGNNNCTG 1 cut(s) 516
AoxI GGCC 1 cut(s) 334
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 1 cut(s) 537
BamHI GGATCC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 402, 434
BbsI GAAGAC 3 cut(s) 316, 403, 503
BccI CCATC 2 cut(s) 85, 479
BcgI CGANNNNNNTGC 2 cut(s) 301, 335
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 1 cut(s) 460
BfaI CTAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 430
BisI GCNGC 2 cut(s) 357, 455
BlsI GCNGC 2 cut(s) 358, 456
Bme1390I CCNGG 1 cut(s) 338
Bme18I GGWCC 1 cut(s) 537
BmgT120I GGNCC 1 cut(s) 537
BmiI GGNNCC 3 cut(s) 290, 376, 538
BmrFI CCNGG 1 cut(s) 338
BpiI GAAGAC 3 cut(s) 316, 403, 503
Bpu10I CCTNAGC 2 cut(s) 208, 331
BpuEI CTTGAG 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 460
Bsc4I CCNNNNNNNGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 86, 222
BseLI CCNNNNNNNGG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 114, 392
BshFI GGCC 1 cut(s) 336
BsiSI CCGG 1 cut(s) 337
BslI CCNNNNNNNGG 1 cut(s) 352
BsmAI GTCTC 1 cut(s) 460
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 460
Bsp143I GATC 3 cut(s) 96, 130, 288
BspACI CCGC 2 cut(s) 356, 454
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 2 cut(s) 113, 393
BspHI TCATGA 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 290, 376, 538
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 2 cut(s) 283, 296
BspTNI GGTCTC 1 cut(s) 460
BssMI GATC 3 cut(s) 96, 130, 288
BssNI GRCGYC 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 382
BstACI GRCGYC 1 cut(s) 305
BstDEI CTNAG 4 cut(s) 100, 208, 331, 401
BstF5I GGATG 2 cut(s) 86, 222
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 3 cut(s) 99, 133, 291
BstMAI GTCTC 1 cut(s) 460
BstMBI GATC 3 cut(s) 96, 130, 288
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNSI RCATGY 2 cut(s) 189, 531
BstSCI CCNGG 1 cut(s) 336
BstV2I GAAGAC 3 cut(s) 316, 403, 503
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 86, 222
BveI ACCTGC 1 cut(s) 430
CaiI CAGNNNCTG 1 cut(s) 516
CciI TCATGA 1 cut(s) 474
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 537
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 170, 419
CspCI CAANNNNNGTGG 2 cut(s) 352, 387
CviAII CATG 4 cut(s) 186, 349, 475, 528
CviJI RGCY 9 cut(s) 30, 146, 212, 330, 336, 345, 359, 375, 435
CviKI_1 RGCY 9 cut(s) 30, 146, 212, 330, 336, 345, 359, 375, 435
CviQI GTAC 2 cut(s) 170, 419
DdeI CTNAG 4 cut(s) 100, 208, 331, 401
DpnI GATC 3 cut(s) 98, 132, 290
DpnII GATC 3 cut(s) 96, 130, 288
Eco31I GGTCTC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 537
EcoT22I ATGCAT 1 cut(s) 529
FaeI CATG 4 cut(s) 189, 352, 478, 531
FatI CATG 4 cut(s) 185, 348, 474, 527
FauNDI CATATG 1 cut(s) 483
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 357, 455
FokI GGATG 2 cut(s) 73, 209
Fsp4HI GCNGC 2 cut(s) 357, 455
FspBI CTAG 1 cut(s) 293
GlaI GCGC 1 cut(s) 458
GluI GCNGC 2 cut(s) 357, 455
HaeIII GGCC 1 cut(s) 336
HapII CCGG 1 cut(s) 337
HgaI GACGC 1 cut(s) 313
HhaI GCGC 1 cut(s) 459
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 4 cut(s) 189, 352, 478, 531
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 2 cut(s) 478, 513
HpaII CCGG 1 cut(s) 337
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 3 cut(s) 69, 103, 512
Hpy188III TCNNGA 2 cut(s) 475, 540
Hpy8I GTNNAC 1 cut(s) 419
Hpy99I CGWCG 2 cut(s) 430, 433
HpyAV CCTTC 4 cut(s) 184, 213, 271, 404
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4V TGCA 3 cut(s) 189, 312, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 4 cut(s) 100, 208, 331, 401
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 4 cut(s) 189, 352, 478, 531
HspAI GCGC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 3 cut(s) 96, 130, 288
LmnI GCTCC 1 cut(s) 380
LpnPI CCDG 4 cut(s) 322, 350, 435, 505
MaeI CTAG 1 cut(s) 293
MalI GATC 3 cut(s) 98, 132, 290
MboI GATC 3 cut(s) 96, 130, 288
MboII GAAGA 5 cut(s) 54, 72, 316, 408, 508
MflI RGATCY 1 cut(s) 288
MluCI AATT 4 cut(s) 8, 32, 118, 447
MnlI CCTC 6 cut(s) 65, 150, 230, 294, 329, 356
Mph1103I ATGCAT 1 cut(s) 529
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 1 cut(s) 486
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 1 cut(s) 338
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 1 cut(s) 338
NdeI CATATG 1 cut(s) 483
NdeII GATC 3 cut(s) 96, 130, 288
NlaIII CATG 4 cut(s) 189, 352, 478, 531
NlaIV GGNNCC 3 cut(s) 290, 376, 538
NsiI ATGCAT 1 cut(s) 529
NspI RCATGY 2 cut(s) 189, 531
PagI TCATGA 1 cut(s) 474
PfeI GAWTC 2 cut(s) 478, 513
PkrI GCNGC 2 cut(s) 358, 456
PpuMI RGGWCCY 1 cut(s) 537
Psp5II RGGWCCY 1 cut(s) 537
PspN4I GGNNCC 3 cut(s) 290, 376, 538
PspPI GGNCC 1 cut(s) 537
PspPPI RGGWCCY 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 516
PsuI RGATCY 1 cut(s) 288
RsaI GTAC 2 cut(s) 171, 420
RsaNI GTAC 2 cut(s) 170, 419
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 2 cut(s) 357, 455
Sau3AI GATC 3 cut(s) 96, 130, 288
Sau96I GGNCC 1 cut(s) 537
ScrFI CCNGG 1 cut(s) 338
SetI ASST 7 cut(s) 57, 114, 142, 205, 263, 424, 437
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 4 cut(s) 8, 32, 118, 447
SsiI CCGC 2 cut(s) 356, 454
SspMI CTAG 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 336
TaaI ACNGT 1 cut(s) 382
TasI AATT 4 cut(s) 8, 32, 118, 447
TauI GCSGC 2 cut(s) 359, 457
TfiI GAWTC 2 cut(s) 478, 513
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TspDTI ATGAA 5 cut(s) 17, 21, 72, 166, 491
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 189, 531
XspI CTAG 1 cut(s) 293
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.