Rmu_sc0000178.1_g000007

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000178.1
OG0004758
Physical Location & Seq
Forward (+)
20513 .. 21237
725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000178.1_g000007.1.cds

Sequence Viewer

Length: 471 bp
atgcatgtggagtttcatatggtgcaaggaattcgaacacgtgcctctggagctgaaggaagttcacacaatcaagatgatccaatttggaaaatccttcggcgtttgaaggtcccatctgctgcaagggtgtttctatggagagctttacatgatattcttccctcttcttttaatctccatcgaagacacttactacattctcatgtctgctcaagctgcaacatggcagctgagactacattacatgctttgtggtattgctccaaggccaaagctgtttggcaggtaagagtacctcaccactggaagacacccccagtccccttcttaaaattgaatatggatggtgcattggaggaaacaacgggtctaatgggattaggggatgctttgctcaatgagataggagaactactcttggcagtttcaaaaggaatgcatggtttctttaatgtgaaggccacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

156

Amino Acids

17.55

Weight (kDa)

9.41

Isoelectric Point (pI)

48.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AclWI GGATC 1 cut(s) 74
AcsI RAATTY 1 cut(s) 30
AcuI CTGAAG 1 cut(s) 75
AcvI CACGTG 1 cut(s) 41
AfaI GTAC 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 3 cut(s) 109, 340, 432
AluBI AGCT 5 cut(s) 53, 146, 219, 233, 278
AluI AGCT 5 cut(s) 53, 146, 219, 233, 278
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 1 cut(s) 74
AoxI GGCC 2 cut(s) 270, 462
ApeKI GCWGC 3 cut(s) 122, 219, 230
ApoI RAATTY 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 293
AsuII TTCGAA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 112
BbrPI CACGTG 1 cut(s) 41
BbsI GAAGAC 2 cut(s) 193, 317
BbvI GCAGC 3 cut(s) 109, 206, 242
BccI CCATC 3 cut(s) 124, 189, 341
BcgI CGANNNNNNTGC 2 cut(s) 14, 48
BcoDI GTCTC 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 277
BisI GCNGC 3 cut(s) 123, 220, 231
BlsI GCNGC 3 cut(s) 124, 221, 232
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 114
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 314
BpiI GAAGAC 2 cut(s) 193, 317
BplI GAGNNNNNCTC 2 cut(s) 402, 434
BpmI CTGGAG 1 cut(s) 69
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 199
BsaAI YACGTR 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 311, 320
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 352, 394
BseMII CTCAG 1 cut(s) 225
BseNI ACTGG 2 cut(s) 311, 320
BseXI GCAGC 3 cut(s) 109, 206, 242
BshFI GGCC 2 cut(s) 272, 464
BslFI GGGAC 2 cut(s) 98, 308
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 2 cut(s) 98, 308
BsmI GAATGC 1 cut(s) 444
BsnI GGCC 2 cut(s) 272, 464
Bsp119I TTCGAA 1 cut(s) 34
Bsp143I GATC 1 cut(s) 79
BspANI GGCC 2 cut(s) 272, 464
BspCNI CTCAG 1 cut(s) 226
BspLI GGNNCC 1 cut(s) 114
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 1 cut(s) 74
BspT104I TTCGAA 1 cut(s) 34
BsrI ACTGG 2 cut(s) 311, 320
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 1 cut(s) 79
BssT1I CCWWGG 1 cut(s) 267
Bst6I CTCTTC 1 cut(s) 172
BstBAI YACGTR 1 cut(s) 41
BstBI TTCGAA 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 352, 394
BstKTI GATC 1 cut(s) 82
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 1 cut(s) 79
BstMWI GCNNNNNNNGC 2 cut(s) 50, 219
BstNSI RCATGY 2 cut(s) 8, 251
BstV1I GCAGC 3 cut(s) 109, 206, 242
BstV2I GAAGAC 2 cut(s) 193, 317
BsuRI GGCC 2 cut(s) 272, 464
BtsCI GGATG 2 cut(s) 352, 394
BtsIMutI CAGTG 1 cut(s) 304
BveI ACCTGC 1 cut(s) 277
Cfr13I GGNCC 1 cut(s) 112
Csp6I GTAC 1 cut(s) 296
CviAII CATG 6 cut(s) 5, 152, 206, 226, 248, 443
CviJI RGCY 7 cut(s) 53, 146, 219, 233, 272, 278, 464
CviKI_1 RGCY 7 cut(s) 53, 146, 219, 233, 272, 278, 464
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 1 cut(s) 234
DpnI GATC 1 cut(s) 81
DpnII GATC 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 75
Eco72I CACGTG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 2 cut(s) 6, 444
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 6 cut(s) 8, 155, 209, 229, 251, 446
FaqI GGGAC 2 cut(s) 98, 308
FatI CATG 6 cut(s) 4, 151, 205, 225, 247, 442
FauNDI CATATG 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 123, 220, 231
FokI GGATG 2 cut(s) 359, 401
Fsp4HI GCNGC 3 cut(s) 123, 220, 231
GluI GCNGC 3 cut(s) 123, 220, 231
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 2 cut(s) 272, 464
Hin1II CATG 6 cut(s) 8, 155, 209, 229, 251, 446
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 65
Hpy188III TCNNGA 2 cut(s) 48, 74
Hpy8I GTNNAC 1 cut(s) 65
HpyAV CCTTC 5 cut(s) 50, 103, 107, 337, 454
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 6 cut(s) 4, 25, 125, 222, 353, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 219
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 6 cut(s) 8, 155, 209, 229, 251, 446
Kzo9I GATC 1 cut(s) 79
LmnI GCTCC 2 cut(s) 50, 269
LpnPI CCDG 4 cut(s) 33, 272, 292, 333
Lsp1109I GCAGC 3 cut(s) 109, 206, 242
LweI GCATC 1 cut(s) 379
MaeII ACGT 1 cut(s) 40
MalI GATC 1 cut(s) 81
MboI GATC 1 cut(s) 79
MboII GAAGA 4 cut(s) 152, 159, 198, 322
MluCI AATT 3 cut(s) 30, 84, 335
MnlI CCTC 4 cut(s) 55, 175, 309, 352
Mph1103I ATGCAT 2 cut(s) 6, 444
MseI TTAA 3 cut(s) 174, 332, 453
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 50, 219
NdeI CATATG 1 cut(s) 18
NdeII GATC 1 cut(s) 79
NlaIII CATG 6 cut(s) 8, 155, 209, 229, 251, 446
NlaIV GGNNCC 1 cut(s) 114
NsiI ATGCAT 2 cut(s) 6, 444
NspI RCATGY 2 cut(s) 8, 251
NspV TTCGAA 1 cut(s) 34
PctI GAATGC 1 cut(s) 444
PkrI GCNGC 3 cut(s) 124, 221, 232
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
Ppu21I YACGTR 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 112
Psp5II RGGWCCY 1 cut(s) 112
PspCI CACGTG 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 114
PspPI GGNCC 1 cut(s) 112
PspPPI RGGWCCY 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 3 cut(s) 174, 332, 453
SatI GCNGC 3 cut(s) 123, 220, 231
Sau3AI GATC 1 cut(s) 79
Sau96I GGNCC 1 cut(s) 112
SetI ASST 9 cut(s) 43, 55, 114, 148, 221, 235, 280, 291, 301
SfaNI GCATC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 214
SmoI CTYRAG 1 cut(s) 214
Sse9I AATT 3 cut(s) 30, 84, 335
StyI CCWWGG 1 cut(s) 267
TaiI ACGT 1 cut(s) 43
TaqI TCGA 2 cut(s) 34, 184
TasI AATT 3 cut(s) 30, 84, 335
Tru1I TTAA 3 cut(s) 174, 332, 453
Tru9I TTAA 3 cut(s) 174, 332, 453
TscAI CASTG 1 cut(s) 311
TseI GCWGC 3 cut(s) 122, 219, 230
TspDTI ATGAA 1 cut(s) 5
TspRI CASTG 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 30
XceI RCATGY 2 cut(s) 8, 251
Zsp2I ATGCAT 2 cut(s) 6, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.