Rh4BG415300

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004758
Physical Location & Seq
Reverse (-)
56328466 .. 56337809
9344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG415300.1

Sequence Viewer

Length: 780 bp
ATGAATGAATTGTCGCCGAACCCAAAAAGCCAATTCTTTATACTCATATCAAACCTCTCATCTTCTCTGAAAACCCCATCTTCATCATCCACCACGATCTCAGAAACCCACCTTTCAAATTCAACTACTGATCAACCTCAAGTCCCAAAATGCCCATCACTTGAAGTACCCTTCATTCCCACATGCACGAAAACCCCACCTTCCTTAGCCAACGCCCTCATCCACTGCGCCACCACAAACATCACCCCACAGCAAACCTTCAAAGATATATCAGTATCAGCAAGGATCCTAGCAAACAGGACGCTCTGCAACTTCCTCGTCTTCGGCTTAGGCCTGGATAGCCTCATGTGGGCTGCTCTCAACAACGGTGGCTCCACTGTTTTCCTAGAAGGAAGAGTGAGATTGGAAGGAAGTGTACCTGCGACGACGAGCTTCAATGACGAAATTGAGCGGCGCAGCAGGAGACCCAGCATGAATCATATGAGTGAAGTGAAGACTACGTGTAGCTATGGATTTCGTGCTATAAAGAAGATCATAGTGGCTGATGAAAATCAAGGAGAATTGTCTGATGAAGATGATGATCTGTGTCCAGTTGAATGTGTAATTGATGAGTATGATGAACAATCCGAGGTTGCAGACTGGATTAGAATTAAGATGGTCCCCCTCTTCCATTTCTATAAAGATGGTGTTCTCTTGGAAGCATTCCCAACTAGAGACGAGGAGAAGATCACTGCGGCAATTCGTAAATACACATCTCCTGCAACATCTGAATTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

259

Amino Acids

28.7

Weight (kDa)

5.02

Isoelectric Point (pI)

55.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 88 - 131 2.5e-13 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 451
AciI CCGC 2 cut(s) 451, 734
AclWI GGATC 2 cut(s) 280, 293
AcsI RAATTY 1 cut(s) 118
AfaI GTAC 2 cut(s) 168, 417
AfiI CCNNNNNNNGG 1 cut(s) 349
AflIII ACRYGT 1 cut(s) 500
AgsI TTSAA 6 cut(s) 117, 123, 164, 262, 436, 596
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 2 cut(s) 432, 507
AluI AGCT 2 cut(s) 432, 507
Alw26I GTCTC 2 cut(s) 457, 708
AlwI GGATC 2 cut(s) 280, 293
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 2 cut(s) 353, 456
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 2 cut(s) 230, 456
AspS9I GGNCC 1 cut(s) 658
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 658
BamHI GGATCC 1 cut(s) 285
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BbsI GAAGAC 2 cut(s) 313, 500
BbvI GCAGC 2 cut(s) 340, 468
BccI CCATC 4 cut(s) 85, 163, 649, 677
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 1 cut(s) 335
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 457, 708
BfaI CTAG 3 cut(s) 290, 386, 711
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 4 cut(s) 354, 452, 457, 735
BlsI GCNGC 4 cut(s) 355, 453, 458, 736
Bme1390I CCNGG 1 cut(s) 335
Bme18I GGWCC 1 cut(s) 658
BmgT120I GGNCC 1 cut(s) 658
BmiI GGNNCC 3 cut(s) 287, 373, 660
BmrFI CCNGG 1 cut(s) 335
BpiI GAAGAC 2 cut(s) 313, 500
Bpu10I CCTNAGC 2 cut(s) 205, 328
BpuEI CTTGAG 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 501
BsaBI GATNNNNATC 2 cut(s) 549, 579
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse1I ACTGG 2 cut(s) 590, 644
Bse8I GATNNNNATC 2 cut(s) 549, 579
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 2 cut(s) 86, 219
BseJI GATNNNNATC 2 cut(s) 549, 579
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 2 cut(s) 590, 644
BseRI GAGGAG 1 cut(s) 734
BseXI GCAGC 2 cut(s) 340, 468
BseYI CCCAGC 1 cut(s) 467
BshFI GGCC 1 cut(s) 333
BslFI GGGAC 2 cut(s) 128, 644
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 2 cut(s) 457, 708
BsmBI CGTCTC 1 cut(s) 708
BsmFI GGGAC 2 cut(s) 128, 644
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 333
Bso31I GGTCTC 1 cut(s) 457
Bsp143I GATC 6 cut(s) 96, 130, 285, 531, 580, 726
BspACI CCGC 2 cut(s) 451, 734
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 1 cut(s) 113
BspLI GGNNCC 3 cut(s) 287, 373, 660
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 280, 293
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 451
BsrI ACTGG 2 cut(s) 590, 644
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 6 cut(s) 96, 130, 285, 531, 580, 726
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 368, 379
Bst6I CTCTTC 2 cut(s) 388, 671
BstBAI YACGTR 1 cut(s) 501
BstDEI CTNAG 3 cut(s) 100, 205, 328
BstF5I GGATG 2 cut(s) 86, 219
BstHHI GCGC 2 cut(s) 230, 456
BstKTI GATC 6 cut(s) 99, 133, 288, 534, 583, 729
BstMAI GTCTC 2 cut(s) 457, 708
BstMBI GATC 6 cut(s) 96, 130, 285, 531, 580, 726
BstMWI GCNNNNNNNGC 1 cut(s) 339
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 340, 468
BstV2I GAAGAC 2 cut(s) 313, 500
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 1 cut(s) 333
BtsCI GGATG 2 cut(s) 86, 219
BtsI GCAGTG 2 cut(s) 223, 729
BtsIMutI CAGTG 3 cut(s) 223, 375, 729
BveI ACCTGC 1 cut(s) 427
CfoI GCGC 2 cut(s) 230, 456
Cfr13I GGNCC 1 cut(s) 658
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 167, 416
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 3 cut(s) 183, 346, 472
CviQI GTAC 2 cut(s) 167, 416
DdeI CTNAG 3 cut(s) 100, 205, 328
DpnI GATC 6 cut(s) 98, 132, 287, 533, 582, 728
DpnII GATC 6 cut(s) 96, 130, 285, 531, 580, 726
Eam1104I CTCTTC 2 cut(s) 388, 671
EarI CTCTTC 2 cut(s) 388, 671
Eco147I AGGCCT 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 3 cut(s) 186, 349, 475
FaqI GGGAC 2 cut(s) 128, 644
FatI CATG 3 cut(s) 182, 345, 471
FauNDI CATATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 4 cut(s) 354, 452, 457, 735
FokI GGATG 2 cut(s) 73, 206
Fsp4HI GCNGC 4 cut(s) 354, 452, 457, 735
FspBI CTAG 3 cut(s) 290, 386, 711
GlaI GCGC 2 cut(s) 229, 455
GluI GCNGC 4 cut(s) 354, 452, 457, 735
GsaI CCCAGC 1 cut(s) 471
HaeIII GGCC 1 cut(s) 333
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 230, 456
Hin1II CATG 3 cut(s) 186, 349, 475
Hin6I GCGC 2 cut(s) 228, 454
HinP1I GCGC 2 cut(s) 228, 454
HinfI GANTC 1 cut(s) 475
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 5 cut(s) 69, 103, 568, 628, 769
Hpy8I GTNNAC 1 cut(s) 416
Hpy99I CGWCG 2 cut(s) 427, 430
HpyAV CCTTC 5 cut(s) 181, 210, 268, 383, 401
HpyCH4III ACNGT 2 cut(s) 368, 379
HpyCH4IV ACGT 1 cut(s) 500
HpyCH4V TGCA 4 cut(s) 186, 309, 635, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 339
HpyF3I CTNAG 3 cut(s) 100, 205, 328
HpySE526I ACGT 1 cut(s) 500
Hsp92II CATG 3 cut(s) 186, 349, 475
HspAI GCGC 2 cut(s) 228, 454
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 6 cut(s) 96, 130, 285, 531, 580, 726
LmnI GCTCC 1 cut(s) 377
LpnPI CCDG 9 cut(s) 283, 320, 347, 432, 445, 481, 603, 625, 771
Lsp1109I GCAGC 2 cut(s) 340, 468
MaeI CTAG 3 cut(s) 290, 386, 711
MaeII ACGT 1 cut(s) 500
MalI GATC 6 cut(s) 98, 132, 287, 533, 582, 728
MbiI CCGCTC 1 cut(s) 451
MboI GATC 6 cut(s) 96, 130, 285, 531, 580, 726
MboII GAAGA 9 cut(s) 54, 72, 313, 405, 505, 541, 584, 658, 736
MflI RGATCY 1 cut(s) 285
MluCI AATT 9 cut(s) 8, 32, 118, 444, 560, 603, 648, 738, 770
MnlI CCTC 8 cut(s) 65, 147, 227, 326, 353, 622, 674, 712
MseI TTAA 1 cut(s) 651
MslI CAYNNNNRTG 1 cut(s) 483
MspR9I CCNGG 1 cut(s) 335
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 339
NdeI CATATG 1 cut(s) 480
NdeII GATC 6 cut(s) 96, 130, 285, 531, 580, 726
NlaIII CATG 3 cut(s) 186, 349, 475
NlaIV GGNNCC 3 cut(s) 287, 373, 660
NspI RCATGY 1 cut(s) 186
PceI AGGCCT 1 cut(s) 333
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 1 cut(s) 475
PkrI GCNGC 4 cut(s) 355, 453, 458, 736
Ppu21I YACGTR 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 3 cut(s) 287, 373, 660
PspPI GGNCC 1 cut(s) 658
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 2 cut(s) 168, 417
RsaNI GTAC 2 cut(s) 167, 416
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 1 cut(s) 651
SatI GCNGC 4 cut(s) 354, 452, 457, 735
Sau3AI GATC 6 cut(s) 96, 130, 285, 531, 580, 726
Sau96I GGNCC 1 cut(s) 658
ScrFI CCNGG 1 cut(s) 335
SinI GGWCC 1 cut(s) 658
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 9 cut(s) 8, 32, 118, 444, 560, 603, 648, 738, 770
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 2 cut(s) 451, 734
SspMI CTAG 3 cut(s) 290, 386, 711
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 368, 379
TaiI ACGT 1 cut(s) 503
TasI AATT 9 cut(s) 8, 32, 118, 444, 560, 603, 648, 738, 770
TauI GCSGC 2 cut(s) 454, 737
TfiI GAWTC 1 cut(s) 475
Tru1I TTAA 1 cut(s) 651
Tru9I TTAA 1 cut(s) 651
TscAI CASTG 3 cut(s) 230, 382, 736
TseI GCWGC 2 cut(s) 353, 456
TspDTI ATGAA 8 cut(s) 17, 21, 72, 163, 488, 561, 585, 633
TspRI CASTG 3 cut(s) 230, 382, 736
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 1 cut(s) 118
XceI RCATGY 1 cut(s) 186
XspI CTAG 3 cut(s) 290, 386, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.