Rh4AG404100

Glucuronoxylan 4-O-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004758
Physical Location & Seq
Reverse (-)
70645489 .. 70646179
691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG404100.1

Sequence Viewer

Length: 540 bp
ATGAATGAATTGTCGCCGAACCCAAAAAGCCAATTCTTTATACTCATATCAAACCTCTCATCTTCTCTGAAAACCCCATCTTCATCATCCACCACGATCTCAGAAACCCACCTTTCAAATTCAACTACTGATCAACCTCAAGTCCCAAAATGCCCATCACTTGAAGTACCCTTCATTCCCACATGCACGAAAACCCCACCTTCCTTAGCCAACGCCCTCATCCACTGCGCCACCACAAACATCACCCCACAGCAAACCTTCAAAGATATATCAGTATCAGCAAGGATCCTAGCAAACAGGACGCTCTGCAACTTCCTCGTCTTCGGCTTAGGCCTGGATAGCCTCATGTGGGCTGCTCTCAACAACGGTGGCTCCACTGTTTTCCTAGAAGGAAGAGTGAGATTGGAAGGAAGTGTACCTGCGACGACGAGCTTCAATGACGAAATTGAGCGGCGCAGCAGGAGACCCAGCATGAATCATATGAGTGAAGTGAAGACTACGGTTTTTAGATTCTGCTGGTATGCATGTAAAGGGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polysacc_synt_4

Protein Analysis

179

Amino Acids

19.62

Weight (kDa)

8.85

Isoelectric Point (pI)

62.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 88 - 131 1.3e-13 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
AccBSI CCGCTC 1 cut(s) 451
AciI CCGC 1 cut(s) 451
AclWI GGATC 2 cut(s) 280, 293
AcsI RAATTY 1 cut(s) 118
AfaI GTAC 2 cut(s) 168, 417
AfiI CCNNNNNNNGG 1 cut(s) 349
AgsI TTSAA 5 cut(s) 117, 123, 164, 262, 436
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 1 cut(s) 432
AluI AGCT 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 457
AlwI GGATC 2 cut(s) 280, 293
AoxI GGCC 1 cut(s) 331
ApeKI GCWGC 2 cut(s) 353, 456
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 2 cut(s) 230, 456
AspS9I GGNCC 1 cut(s) 534
AsuHPI GGTGA 1 cut(s) 235
AvaII GGWCC 1 cut(s) 534
BamHI GGATCC 1 cut(s) 285
BarI GAAGNNNNNNTAC 2 cut(s) 399, 431
BbsI GAAGAC 2 cut(s) 313, 500
BbvI GCAGC 2 cut(s) 340, 468
BccI CCATC 2 cut(s) 85, 163
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 1 cut(s) 335
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 290, 386
BfuAI ACCTGC 1 cut(s) 427
BisI GCNGC 3 cut(s) 354, 452, 457
BlsI GCNGC 3 cut(s) 355, 453, 458
Bme1390I CCNGG 1 cut(s) 335
Bme18I GGWCC 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 534
BmiI GGNNCC 3 cut(s) 287, 373, 535
BmrFI CCNGG 1 cut(s) 335
BpiI GAAGAC 2 cut(s) 313, 500
Bpu10I CCTNAGC 2 cut(s) 205, 328
BpuEI CTTGAG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 457
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 335
BseGI GGATG 2 cut(s) 86, 219
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 340, 468
BseYI CCCAGC 1 cut(s) 467
BshFI GGCC 1 cut(s) 333
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 1 cut(s) 457
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 333
Bso31I GGTCTC 1 cut(s) 457
Bsp143I GATC 3 cut(s) 96, 130, 285
BspACI CCGC 1 cut(s) 451
BspANI GGCC 1 cut(s) 333
BspCNI CTCAG 1 cut(s) 113
BspLI GGNNCC 3 cut(s) 287, 373, 535
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 280, 293
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 451
BssMI GATC 3 cut(s) 96, 130, 285
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 3 cut(s) 368, 379, 502
Bst6I CTCTTC 1 cut(s) 388
BstDEI CTNAG 3 cut(s) 100, 205, 328
BstF5I GGATG 2 cut(s) 86, 219
BstHHI GCGC 2 cut(s) 230, 456
BstKTI GATC 3 cut(s) 99, 133, 288
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 3 cut(s) 96, 130, 285
BstMWI GCNNNNNNNGC 1 cut(s) 339
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 2 cut(s) 186, 528
BstSCI CCNGG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 340, 468
BstV2I GAAGAC 2 cut(s) 313, 500
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 1 cut(s) 333
BtsCI GGATG 2 cut(s) 86, 219
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 223, 375
BveI ACCTGC 1 cut(s) 427
CfoI GCGC 2 cut(s) 230, 456
Cfr13I GGNCC 1 cut(s) 534
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 167, 416
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 4 cut(s) 183, 346, 472, 525
CviJI RGCY 8 cut(s) 30, 209, 327, 333, 342, 353, 372, 432
CviKI_1 RGCY 8 cut(s) 30, 209, 327, 333, 342, 353, 372, 432
CviQI GTAC 2 cut(s) 167, 416
DdeI CTNAG 3 cut(s) 100, 205, 328
DpnI GATC 3 cut(s) 98, 132, 287
DpnII GATC 3 cut(s) 96, 130, 285
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Eco147I AGGCCT 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 526
FaeI CATG 4 cut(s) 186, 349, 475, 528
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 182, 345, 471, 524
FauNDI CATATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 3 cut(s) 354, 452, 457
FokI GGATG 2 cut(s) 73, 206
Fsp4HI GCNGC 3 cut(s) 354, 452, 457
FspBI CTAG 2 cut(s) 290, 386
GlaI GCGC 2 cut(s) 229, 455
GluI GCNGC 3 cut(s) 354, 452, 457
GsaI CCCAGC 1 cut(s) 471
HaeIII GGCC 1 cut(s) 333
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 230, 456
Hin1II CATG 4 cut(s) 186, 349, 475, 528
Hin6I GCGC 2 cut(s) 228, 454
HinP1I GCGC 2 cut(s) 228, 454
HinfI GANTC 2 cut(s) 475, 510
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 69, 103
Hpy188III TCNNGA 1 cut(s) 537
Hpy8I GTNNAC 1 cut(s) 416
Hpy99I CGWCG 2 cut(s) 427, 430
HpyAV CCTTC 5 cut(s) 181, 210, 268, 383, 401
HpyCH4III ACNGT 3 cut(s) 368, 379, 502
HpyCH4V TGCA 3 cut(s) 186, 309, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 339
HpyF3I CTNAG 3 cut(s) 100, 205, 328
Hsp92II CATG 4 cut(s) 186, 349, 475, 528
HspAI GCGC 2 cut(s) 228, 454
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 3 cut(s) 96, 130, 285
LmnI GCTCC 1 cut(s) 377
LpnPI CCDG 7 cut(s) 283, 320, 347, 432, 445, 481, 502
Lsp1109I GCAGC 2 cut(s) 340, 468
MaeI CTAG 2 cut(s) 290, 386
MalI GATC 3 cut(s) 98, 132, 287
MbiI CCGCTC 1 cut(s) 451
MboI GATC 3 cut(s) 96, 130, 285
MboII GAAGA 5 cut(s) 54, 72, 313, 405, 505
MflI RGATCY 1 cut(s) 285
MluCI AATT 4 cut(s) 8, 32, 118, 444
MnlI CCTC 5 cut(s) 65, 147, 227, 326, 353
Mph1103I ATGCAT 1 cut(s) 526
MslI CAYNNNNRTG 1 cut(s) 483
MspR9I CCNGG 1 cut(s) 335
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 339
NdeI CATATG 1 cut(s) 480
NdeII GATC 3 cut(s) 96, 130, 285
NlaIII CATG 4 cut(s) 186, 349, 475, 528
NlaIV GGNNCC 3 cut(s) 287, 373, 535
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 2 cut(s) 186, 528
PceI AGGCCT 1 cut(s) 333
PfeI GAWTC 2 cut(s) 475, 510
PkrI GCNGC 3 cut(s) 355, 453, 458
PpuMI RGGWCCY 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 3 cut(s) 287, 373, 535
PspPI GGNCC 1 cut(s) 534
PspPPI RGGWCCY 1 cut(s) 534
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 2 cut(s) 168, 417
RsaNI GTAC 2 cut(s) 167, 416
RseI CAYNNNNRTG 1 cut(s) 483
SatI GCNGC 3 cut(s) 354, 452, 457
Sau3AI GATC 3 cut(s) 96, 130, 285
Sau96I GGNCC 1 cut(s) 534
ScrFI CCNGG 1 cut(s) 335
SetI ASST 7 cut(s) 57, 114, 139, 202, 260, 421, 434
SinI GGWCC 1 cut(s) 534
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 4 cut(s) 8, 32, 118, 444
SseBI AGGCCT 1 cut(s) 333
SsiI CCGC 1 cut(s) 451
SspMI CTAG 2 cut(s) 290, 386
StuI AGGCCT 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 333
TaaI ACNGT 3 cut(s) 368, 379, 502
TasI AATT 4 cut(s) 8, 32, 118, 444
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 2 cut(s) 475, 510
TscAI CASTG 2 cut(s) 230, 382
TseI GCWGC 2 cut(s) 353, 456
TspDTI ATGAA 5 cut(s) 17, 21, 72, 163, 488
TspRI CASTG 2 cut(s) 230, 382
VpaK11BI GGWCC 1 cut(s) 534
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 186, 528
XspI CTAG 2 cut(s) 290, 386
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.