Rw7G025900

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004758
Physical Location & Seq
Forward (+)
32738816 .. 32754609
15794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025900.1

Sequence Viewer

Length: 594 bp
ATGGAACCGTTGAACCGTTTCGGAAACGGCAGCGGCGGGTCTCCGTCAGGCCGAGTCCGAAAATTGTTTAACGCCGGTGGACGGTGGAGGAAGGAAGAAGACAAAAGCTCACCAAGGCGGATCCCTAGGCCGGGAAACTTTAAGCCTTACTCCCCACCTTATTTGGCTATGGATCATCCGGTGAGTATATTGATGACAGCGACCGTAGGTTGGAATGAAGCTTTTGTACATGCTAATTTCTTTCCAGGGCATGCTGATGTTATTCTATCAATTCCTTTACCAGGAAGACAACATGCTGATAAATGGGTTTGGCACTGTATAACAAAGGAAATGTACACGGTTGCTAGTGGTTATCAGTTGGTCGTGGACTTTGAAAGACAAGAAAGTGCAGTTGGGGAAGGTTCTGATAATGAGAGAACGAAGGGAATATGGCAGAAGCGAGGGGAAAACGAAACCCCAATTCGTCGAAGAATTCCTTTTGTTCTGATCTATCCTCGACGAACGGAAGCGCCAACTCTGAAGAGCGCCTCTCAGGATTCCAATTCAGATTTCTCGTTCCGCAATAAGATACCCCTTCTAGGGTTTCCTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

197

Amino Acids

22.33

Weight (kDa)

9.71

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 36, 118, 559
AclWI GGATC 3 cut(s) 115, 128, 180
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 539
AfaI GTAC 2 cut(s) 228, 335
AfiI CCNNNNNNNGG 7 cut(s) 81, 130, 131, 210, 281, 578, 579
AgsI TTSAA 2 cut(s) 13, 374
AjnI CCWGG 2 cut(s) 244, 280
AjuI GAANNNNNNNTTGG 4 cut(s) 375, 407, 533, 565
AluBI AGCT 2 cut(s) 108, 221
AluI AGCT 2 cut(s) 108, 221
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 3 cut(s) 115, 128, 180
AoxI GGCC 2 cut(s) 49, 128
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 1 cut(s) 471
Asp700I GAANNNNTTC 1 cut(s) 17
AspA2I CCTAGG 1 cut(s) 125
AspLEI GCGC 2 cut(s) 511, 527
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 102, 193
AvrII CCTAGG 1 cut(s) 125
BamHI GGATCC 1 cut(s) 120
BarI GAAGNNNNNNTAC 2 cut(s) 210, 242
BbsI GAAGAC 2 cut(s) 105, 292
BbvI GCAGC 1 cut(s) 42
BceAI ACGGC 1 cut(s) 43
BciT130I CCWGG 2 cut(s) 246, 282
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 3 cut(s) 126, 345, 578
BfoI RGCGCY 2 cut(s) 512, 528
BisI GCNGC 2 cut(s) 31, 34
BlnI CCTAGG 1 cut(s) 125
BlsI GCNGC 2 cut(s) 32, 35
Bme1390I CCNGG 3 cut(s) 132, 246, 282
BmiI GGNNCC 2 cut(s) 6, 122
BmrFI CCNGG 3 cut(s) 132, 246, 282
BpiI GAAGAC 2 cut(s) 105, 292
BplI GAGNNNNNCTC 2 cut(s) 514, 546
BpuMI CCSGG 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 3 cut(s) 113, 125, 245
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 7 cut(s) 81, 130, 131, 210, 281, 578, 579
Bse118I RCCGGY 1 cut(s) 74
BseBI CCWGG 2 cut(s) 246, 282
BseDI CCNNGG 3 cut(s) 113, 125, 245
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 7 cut(s) 81, 130, 131, 210, 281, 578, 579
BseMII CTCAG 1 cut(s) 545
BseXI GCAGC 1 cut(s) 42
BsgI GTGCAG 1 cut(s) 408
Bsh1285I CGRYCG 1 cut(s) 204
BshFI GGCC 2 cut(s) 51, 130
BsiEI CGRYCG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 75, 131, 179
BslI CCNNNNNNNGG 7 cut(s) 81, 130, 131, 210, 281, 578, 579
BsmAI GTCTC 1 cut(s) 45
BsnI GGCC 2 cut(s) 51, 130
Bso31I GGTCTC 1 cut(s) 45
Bsp1407I TGTACA 2 cut(s) 226, 333
Bsp143I GATC 3 cut(s) 120, 172, 486
BspACI CCGC 4 cut(s) 33, 36, 118, 559
BspANI GGCC 2 cut(s) 51, 130
BspCNI CTCAG 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 6, 122
BspPI GGATC 3 cut(s) 115, 128, 180
BspQI GCTCTTC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 74
BsrGI TGTACA 2 cut(s) 226, 333
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 113, 125, 245
BssMI GATC 3 cut(s) 120, 172, 486
BssT1I CCWWGG 2 cut(s) 113, 125
Bst2UI CCWGG 2 cut(s) 246, 282
Bst4CI ACNGT 6 cut(s) 9, 17, 84, 205, 317, 340
Bst6I CTCTTC 1 cut(s) 515
BstAUI TGTACA 2 cut(s) 226, 333
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 531
BstENI CCTNNNNNAGG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 175
BstH2I RGCGCY 2 cut(s) 512, 528
BstHHI GCGC 2 cut(s) 511, 527
BstKTI GATC 3 cut(s) 123, 175, 489
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 3 cut(s) 120, 172, 486
BstMCI CGRYCG 1 cut(s) 204
BstNI CCWGG 2 cut(s) 246, 282
BstNSI RCATGY 3 cut(s) 233, 254, 296
BstSCI CCNGG 3 cut(s) 130, 244, 280
BstV1I GCAGC 1 cut(s) 42
BstV2I GAAGAC 2 cut(s) 105, 292
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 2 cut(s) 51, 130
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 2 cut(s) 511, 527
Cfr10I RCCGGY 1 cut(s) 74
Csp6I GTAC 2 cut(s) 227, 334
CspCI CAANNNNNGTGG 2 cut(s) 144, 179
CviAII CATG 3 cut(s) 230, 251, 293
CviJI RGCY 6 cut(s) 51, 108, 130, 145, 167, 221
CviKI_1 RGCY 6 cut(s) 51, 108, 130, 145, 167, 221
CviQI GTAC 2 cut(s) 227, 334
DdeI CTNAG 1 cut(s) 531
DpnI GATC 3 cut(s) 122, 174, 488
DpnII GATC 3 cut(s) 120, 172, 486
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 133
Eco130I CCWWGG 2 cut(s) 113, 125
Eco31I GGTCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 279
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 244, 280
EcoT14I CCWWGG 2 cut(s) 113, 125
ErhI CCWWGG 2 cut(s) 113, 125
FaeI CATG 3 cut(s) 233, 254, 296
FaiI YATR 7 cut(s) 170, 188, 231, 252, 294, 320, 430
FalI AAGNNNNNCTT 2 cut(s) 460, 492
FatI CATG 3 cut(s) 229, 250, 292
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 31, 34
FokI GGATG 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 31, 34
FspBI CTAG 3 cut(s) 126, 345, 578
GlaI GCGC 2 cut(s) 510, 526
GluI GCNGC 2 cut(s) 31, 34
HaeII RGCGCY 2 cut(s) 512, 528
HaeIII GGCC 2 cut(s) 51, 130
HapII CCGG 3 cut(s) 75, 131, 179
HhaI GCGC 2 cut(s) 511, 527
Hin1II CATG 3 cut(s) 233, 254, 296
Hin6I GCGC 2 cut(s) 509, 525
HinP1I GCGC 2 cut(s) 509, 525
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 2 cut(s) 54, 536
HpaII CCGG 3 cut(s) 75, 131, 179
HphI GGTGA 2 cut(s) 102, 193
Hpy166II GTNNAC 3 cut(s) 80, 336, 367
Hpy188I TCNGA 6 cut(s) 23, 59, 406, 486, 519, 547
Hpy188III TCNNGA 2 cut(s) 533, 587
Hpy8I GTNNAC 3 cut(s) 80, 336, 367
Hpy99I CGWCG 2 cut(s) 468, 501
HpyAV CCTTC 4 cut(s) 85, 392, 415, 584
HpyCH4III ACNGT 6 cut(s) 9, 17, 84, 205, 317, 340
HpyCH4V TGCA 1 cut(s) 389
HpyF3I CTNAG 1 cut(s) 531
Hsp92II CATG 3 cut(s) 233, 254, 296
HspAI GCGC 2 cut(s) 509, 525
Kzo9I GATC 3 cut(s) 120, 172, 486
LguI GCTCTTC 1 cut(s) 515
LpnPI CCDG 9 cut(s) 33, 88, 144, 192, 231, 258, 267, 294, 518
Lsp1109I GCAGC 1 cut(s) 42
MaeI CTAG 3 cut(s) 126, 345, 578
MalI GATC 3 cut(s) 122, 174, 488
MboI GATC 3 cut(s) 120, 172, 486
MboII GAAGA 5 cut(s) 107, 110, 297, 480, 532
MflI RGATCY 1 cut(s) 120
MluCI AATT 6 cut(s) 62, 235, 270, 459, 471, 541
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 4 cut(s) 81, 434, 504, 538
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 2 cut(s) 69, 141
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 33
MspI CCGG 3 cut(s) 75, 131, 179
MspR9I CCNGG 3 cut(s) 132, 246, 282
MvaI CCWGG 2 cut(s) 246, 282
NciI CCSGG 1 cut(s) 132
NdeII GATC 3 cut(s) 120, 172, 486
NlaIII CATG 3 cut(s) 233, 254, 296
NlaIV GGNNCC 2 cut(s) 6, 122
NmeAIII GCCGAG 1 cut(s) 77
NspI RCATGY 3 cut(s) 233, 254, 296
PaeI GCATGC 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 515
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 536
PkrI GCNGC 2 cut(s) 32, 35
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 244, 280
PspGI CCWGG 2 cut(s) 244, 280
PspN4I GGNNCC 2 cut(s) 6, 122
PsuI RGATCY 1 cut(s) 120
RsaI GTAC 2 cut(s) 228, 335
RsaNI GTAC 2 cut(s) 227, 334
RseI CAYNNNNRTG 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 2 cut(s) 69, 141
SatI GCNGC 2 cut(s) 31, 34
Sau3AI GATC 3 cut(s) 120, 172, 486
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 3 cut(s) 132, 246, 282
SetI ASST 5 cut(s) 110, 160, 211, 223, 403
SgrAI CRCCGGYG 1 cut(s) 74
SmiMI CAYNNNNRTG 1 cut(s) 255
SphI GCATGC 1 cut(s) 254
Sse9I AATT 6 cut(s) 62, 235, 270, 459, 471, 541
SsiI CCGC 4 cut(s) 33, 36, 118, 559
SspMI CTAG 3 cut(s) 126, 345, 578
StyD4I CCNGG 3 cut(s) 130, 244, 280
StyI CCWWGG 2 cut(s) 113, 125
TaaI ACNGT 6 cut(s) 9, 17, 84, 205, 317, 340
TaqI TCGA 2 cut(s) 466, 496
TasI AATT 6 cut(s) 62, 235, 270, 459, 471, 541
TatI WGTACW 2 cut(s) 226, 333
TauI GCSGC 1 cut(s) 36
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 2 cut(s) 69, 141
Tru9I TTAA 2 cut(s) 69, 141
TscAI CASTG 1 cut(s) 320
TseI GCWGC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 2 cut(s) 33, 518
TspRI CASTG 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 279
XapI RAATTY 1 cut(s) 471
XceI RCATGY 3 cut(s) 233, 254, 296
XmaJI CCTAGG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 3 cut(s) 126, 345, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.