Rroxscaffold_6G00423780

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0004758
Physical Location & Seq
Forward (+)
44904860 .. 44909253
4394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423780.1

Sequence Viewer

Length: 777 bp
ATGGGGCCGCATTGGTCGGTTTCGACACAAAGGGTGGTGGAGCAAGTGGTCGGGGCCGAGGTCAAGGAGGGTTTGGGTGCCCGAAATTTTGGTGTCCAAATGAGTTTCCCGTGGGCTTGGGCCTCTGCCCTTTTGGATTTCTCAAGTAGGGGTTGGCCCTATTCTCTTGAGACTTTGCCTTTTGTGTATGAACCCGATGTTGTTAGGAGAGATGGAGTGGTGGAAAGGCGACGCCGCCGGAAGTTCGGATGCGGCGGCGGCGATGGCTTGGCAATGTGTTTGGGCTTTTGTTTCAAGAACTCGAATTTAGGCTTGGGCCTTTGGGCTGTGACCAATTTGAGTCCGCAGATTTCAAAGAAGAGCAAGAAGACCAACCTGTTGGCCGAGTTTCTCCGTGCTCATTTCGATCAGGGAGATGTAGAGAGCGTTATTAATTCTATTCCATTAGGAGGAAGACAATGCCTTGACAAATGGATTTGGCACTACACTTTAAGAGGTATGTATACTGTAGTAAGTGGTTACAATATGATTGTGCAGTTAAATTCTTTAGAGCAAGAGTGCAGCAGCCGCTCCAGTGAAGAGAAACTTACTGATTTCTTGAAGAGACTTTGGGGGGTTCAAGTTCCAGAGAAGATTAAGGTGTTTATGTGGCGTGCCTCGAAAGAAATTCTTCTATGTTTGGGAAATTTGGCCAAGAGGAGAATACCGGTGAATCAAAGATGCCCAAGATGTGGAAGAGAAGAAGAATCAGTAGTTTTATGCTCTATTGTACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

258

Amino Acids

29.22

Weight (kDa)

9.28

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 195 - 251 3.2e-10 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 731
AccBSI CCGCTC 1 cut(s) 570
AccI GTMKAC 1 cut(s) 503
AciI CCGC 7 cut(s) 8, 235, 252, 255, 258, 344, 568
AcoI YGGCCR 2 cut(s) 381, 690
AcsI RAATTY 5 cut(s) 85, 304, 541, 666, 685
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 1 cut(s) 771
AfiI CCNNNNNNNGG 2 cut(s) 449, 731
AgeI ACCGGT 1 cut(s) 706
AgsI TTSAA 4 cut(s) 295, 354, 601, 620
AjuI GAANNNNNNNTTGG 2 cut(s) 296, 328
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 2 cut(s) 164, 598
AoxI GGCC 7 cut(s) 5, 54, 120, 155, 316, 381, 690
ApeKI GCWGC 2 cut(s) 561, 564
ApoI RAATTY 5 cut(s) 85, 304, 541, 666, 685
AseI ATTAAT 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 706
Asp700I GAANNNNTTC 1 cut(s) 669
AspS9I GGNCC 5 cut(s) 5, 54, 120, 156, 316
AsuHPI GGTGA 1 cut(s) 721
BaeGI GKGCMC 1 cut(s) 82
BalI TGGCCA 1 cut(s) 692
BanI GGYRCC 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 374, 460
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 2 cut(s) 573, 576
BccI CCATC 2 cut(s) 206, 257
BcoDI GTCTC 2 cut(s) 164, 598
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 8 cut(s) 8, 235, 253, 256, 259, 562, 565, 568
BlsI GCNGC 8 cut(s) 9, 236, 254, 257, 260, 563, 566, 569
BmgT120I GGNCC 5 cut(s) 5, 54, 120, 156, 316
BmiI GGNNCC 3 cut(s) 6, 55, 79
BmsI GCATC 2 cut(s) 239, 710
BpiI GAAGAC 2 cut(s) 374, 460
BpmI CTGGAG 1 cut(s) 556
BpuEI CTTGAG 2 cut(s) 127, 188
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 57, 110
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 2 cut(s) 449, 731
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 57, 110
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 449, 731
BseMI GCAATG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 712
BseSI GKGCMC 1 cut(s) 82
BseXI GCAGC 2 cut(s) 573, 576
BsgI GTGCAG 2 cut(s) 554, 580
BshFI GGCC 7 cut(s) 7, 56, 122, 157, 318, 383, 692
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 2 cut(s) 238, 707
BslI CCNNNNNNNGG 2 cut(s) 449, 731
BsmAI GTCTC 2 cut(s) 164, 598
BsnI GGCC 7 cut(s) 7, 56, 122, 157, 318, 383, 692
Bsp1286I GDGCHC 2 cut(s) 82, 400
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 7 cut(s) 8, 235, 252, 255, 258, 344, 568
BspANI GGCC 7 cut(s) 7, 56, 122, 157, 318, 383, 692
BspLI GGNNCC 3 cut(s) 6, 55, 79
BspQI GCTCTTC 1 cut(s) 353
BspT107I GGYRCC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 570
BsrDI GCAATG 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 706
BsrI ACTGG 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 2 cut(s) 57, 110
BssMI GATC 1 cut(s) 406
BssNAI GTATAC 1 cut(s) 504
BssNI GRCGYC 1 cut(s) 232
Bst1107I GTATAC 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 508
Bst6I CTCTTC 4 cut(s) 353, 573, 596, 730
BstACI GRCGYC 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 654
BstDSI CCRYGG 1 cut(s) 110
BstF5I GGATG 1 cut(s) 254
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 2 cut(s) 164, 598
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 3 cut(s) 258, 264, 567
BstSFI CTRYAG 1 cut(s) 507
BstSLI GKGCMC 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 573, 576
BstV2I GAAGAC 2 cut(s) 374, 460
BstXI CCANNNNNNTGG 1 cut(s) 379
BstZ17I GTATAC 1 cut(s) 504
BsuRI GGCC 7 cut(s) 7, 56, 122, 157, 318, 383, 692
BtgI CCRYGG 1 cut(s) 110
BtgZI GCGATG 1 cut(s) 276
BtsCI GGATG 1 cut(s) 254
BtsIMutI CAGTG 1 cut(s) 580
Cac8I GCNNGC 1 cut(s) 654
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 5 cut(s) 5, 54, 120, 156, 316
CseI GACGC 1 cut(s) 240
Csp6I GTAC 1 cut(s) 770
CspAI ACCGGT 1 cut(s) 706
CviQI GTAC 1 cut(s) 770
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
EaeI YGGCCR 2 cut(s) 381, 690
Eam1104I CTCTTC 4 cut(s) 353, 573, 596, 730
EarI CTCTTC 4 cut(s) 353, 573, 596, 730
FaiI YATR 8 cut(s) 189, 500, 504, 527, 647, 676, 760, 775
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 654, 686
FblI GTMKAC 1 cut(s) 503
Fnu4HI GCNGC 8 cut(s) 8, 235, 253, 256, 259, 562, 565, 568
FokI GGATG 1 cut(s) 261
Fsp4HI GCNGC 8 cut(s) 8, 235, 253, 256, 259, 562, 565, 568
GluI GCNGC 8 cut(s) 8, 235, 253, 256, 259, 562, 565, 568
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 7 cut(s) 7, 56, 122, 157, 318, 383, 692
HapII CCGG 2 cut(s) 238, 707
HgaI GACGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 232
HinfI GANTC 3 cut(s) 340, 712, 746
HpaII CCGG 2 cut(s) 238, 707
HphI GGTGA 1 cut(s) 721
Hpy166II GTNNAC 1 cut(s) 504
Hpy188I TCNGA 1 cut(s) 248
Hpy188III TCNNGA 4 cut(s) 167, 295, 598, 626
Hpy8I GTNNAC 1 cut(s) 504
Hpy99I CGWCG 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 508
HpyCH4V TGCA 2 cut(s) 535, 561
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 264, 567
Hsp92I GRCGYC 1 cut(s) 232
Kzo9I GATC 1 cut(s) 406
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 2 cut(s) 40, 575
LpnPI CCDG 6 cut(s) 251, 389, 395, 586, 639, 720
Lsp1109I GCAGC 2 cut(s) 573, 576
LweI GCATC 2 cut(s) 239, 710
MaeIII GTNAC 2 cut(s) 328, 518
MalI GATC 1 cut(s) 408
MbiI CCGCTC 1 cut(s) 570
MboI GATC 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 82, 400
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 7 cut(s) 85, 304, 334, 433, 541, 666, 685
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 1 cut(s) 349
MnlI CCTC 7 cut(s) 52, 61, 133, 443, 488, 667, 690
Mox20I TGGCCA 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 692
MseI TTAA 4 cut(s) 432, 491, 539, 636
Msp20I TGGCCA 1 cut(s) 692
MspI CCGG 2 cut(s) 238, 707
MwoI GCNNNNNNNGC 3 cut(s) 258, 264, 567
NdeII GATC 1 cut(s) 406
NlaIV GGNNCC 3 cut(s) 6, 55, 79
NmeAIII GCCGAG 2 cut(s) 82, 409
NmuCI GTSAC 1 cut(s) 328
PciSI GCTCTTC 1 cut(s) 353
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 2 cut(s) 712, 746
PflMI CCANNNNNTGG 1 cut(s) 731
PinAI ACCGGT 1 cut(s) 706
PkrI GCNGC 8 cut(s) 9, 236, 254, 257, 260, 563, 566, 569
PleI GAGTC 1 cut(s) 348
PpsI GAGTC 1 cut(s) 348
PshBI ATTAAT 1 cut(s) 432
PspN4I GGNNCC 3 cut(s) 6, 55, 79
PspPI GGNCC 5 cut(s) 5, 54, 120, 156, 316
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 4 cut(s) 432, 491, 539, 636
SatI GCNGC 8 cut(s) 8, 235, 253, 256, 259, 562, 565, 568
Sau3AI GATC 1 cut(s) 406
Sau96I GGNCC 5 cut(s) 5, 54, 120, 156, 316
SchI GAGTC 1 cut(s) 349
SduI GDGCHC 2 cut(s) 82, 400
SetI ASST 4 cut(s) 63, 378, 499, 642
SfaNI GCATC 2 cut(s) 239, 710
SfcI CTRYAG 1 cut(s) 507
SmlI CTYRAG 2 cut(s) 142, 167
SmoI CTYRAG 2 cut(s) 142, 167
Sse9I AATT 7 cut(s) 85, 304, 334, 433, 541, 666, 685
SsiI CCGC 7 cut(s) 8, 235, 252, 255, 258, 344, 568
TaaI ACNGT 1 cut(s) 508
TaqI TCGA 4 cut(s) 23, 302, 405, 659
TasI AATT 7 cut(s) 85, 304, 334, 433, 541, 666, 685
TatI WGTACW 1 cut(s) 769
TauI GCSGC 6 cut(s) 10, 237, 255, 258, 261, 570
TfiI GAWTC 2 cut(s) 712, 746
Tru1I TTAA 4 cut(s) 432, 491, 539, 636
Tru9I TTAA 4 cut(s) 432, 491, 539, 636
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 2 cut(s) 561, 564
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 1 cut(s) 580
Van91I CCANNNNNTGG 1 cut(s) 731
VspI ATTAAT 1 cut(s) 432
XapI RAATTY 5 cut(s) 85, 304, 541, 666, 685
XmiI GTMKAC 1 cut(s) 503
XmnI GAANNNNTTC 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.