Rh4DG306300

ribonuclease H protein At1g65750-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004758
Physical Location & Seq
Reverse (-)
52789334 .. 52799482
10149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG306300.1

Sequence Viewer

Length: 585 bp
ATGGCAGCGGATGTCTCCGCGCAGGAGTACGGGAACAGGAAACGTCAAACGGGTGTGATTATAAACAATGTAGGCTCCGAATCGTTCCACGTGGTGCTGTTTTTCCCCACACATTTTAACTGTCTCTTCCCAAATCTTGCCATCTCCCGCGTTAGATCCGTCATTCGCGGGAATTTAACTGTTGTTTTCCACCCAATCGCGGTAAGAAGGGTTAAAAAGATCGAGCTATATGAAATGGTCGGTTTGGAGCATGTTGGGGTTAAAATGAGAGCACATGTTCGTGTCTCCCATCTGGAATTGAGAGAGAAAACAATTGGCAAAGGATCTGATATGAAGGTGATGGAAAAGATGTGGCAAAGGCTGTGGAAACTAAAGATTCCAGAGAGAGTTAAAATTTTCATGTGGCGGGCTATGAAGGATATTCTCCCATGCAAAGCTAACCTAAATAGACGTCACATTATGGTGGATGCATCTTGTCCACGTTGTGGATGCCAAGGTGAGACAAGTATTCGTGCTCTCTTTAATTGTAAGCATGCAAAAATGATGTGGGCTTTATCTCCTTTCAGTAGCATAATTTCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

194

Amino Acids

22.34

Weight (kDa)

10.26

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 114 - 183 5.6e-15 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 62
AatII GACGTC 1 cut(s) 454
AccB7I CCANNNNNTGG 1 cut(s) 485
AccII CGCG 4 cut(s) 20, 150, 168, 200
AciI CCGC 6 cut(s) 8, 18, 148, 168, 200, 406
AclWI GGATC 2 cut(s) 150, 331
AcsI RAATTY 2 cut(s) 172, 393
AcvI CACGTG 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 451
AdeI CACNNNGTG 2 cut(s) 94, 485
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 199, 485
AflIII ACRYGT 1 cut(s) 274
AluBI AGCT 2 cut(s) 226, 437
AluI AGCT 2 cut(s) 226, 437
Alw21I GWGCWC 2 cut(s) 274, 517
Alw26I GTCTC 4 cut(s) 19, 128, 289, 494
AlwI GGATC 2 cut(s) 150, 331
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 172, 393
AspLEI GCGC 1 cut(s) 22
AsuHPI GGTGA 2 cut(s) 349, 509
BbrPI CACGTG 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 274, 517
BbvI GCAGC 1 cut(s) 17
BccI CCATC 3 cut(s) 149, 297, 334
BcoDI GTCTC 4 cut(s) 19, 128, 289, 494
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 76
BmsI GCATC 3 cut(s) 457, 479, 479
BsaAI YACGTR 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 485
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 3 cut(s) 16, 472, 494
BseLI CCNNNNNNNGG 2 cut(s) 199, 485
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 4 cut(s) 20, 150, 168, 200
BsiHKAI GWGCWC 2 cut(s) 274, 517
BslI CCNNNNNNNGG 2 cut(s) 199, 485
BsmAI GTCTC 4 cut(s) 19, 128, 289, 494
Bsp1286I GDGCHC 2 cut(s) 274, 517
Bsp143I GATC 3 cut(s) 155, 219, 323
BspACI CCGC 6 cut(s) 8, 18, 148, 168, 200, 406
BspFNI CGCG 4 cut(s) 20, 150, 168, 200
BspLI GGNNCC 1 cut(s) 76
BspPI GGATC 2 cut(s) 150, 331
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 3 cut(s) 155, 219, 323
BssNI GRCGYC 1 cut(s) 451
BssT1I CCWWGG 1 cut(s) 493
Bst4CI ACNGT 2 cut(s) 122, 181
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 1 cut(s) 451
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 408, 534
BstF5I GGATG 3 cut(s) 16, 472, 494
BstFNI CGCG 4 cut(s) 20, 150, 168, 200
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 3 cut(s) 158, 222, 326
BstMAI GTCTC 4 cut(s) 19, 128, 289, 494
BstMBI GATC 3 cut(s) 155, 219, 323
BstNSI RCATGY 3 cut(s) 254, 278, 536
BstUI CGCG 4 cut(s) 20, 150, 168, 200
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 155, 323
BstYI RGATCY 2 cut(s) 155, 323
BtsCI GGATG 3 cut(s) 16, 472, 494
Cac8I GCNNGC 2 cut(s) 408, 534
CfoI GCGC 1 cut(s) 22
Csp6I GTAC 1 cut(s) 28
CspCI CAANNNNNGTGG 2 cut(s) 344, 379
CviAII CATG 5 cut(s) 251, 275, 400, 429, 533
CviJI RGCY 6 cut(s) 75, 226, 361, 410, 437, 551
CviKI_1 RGCY 6 cut(s) 75, 226, 361, 410, 437, 551
CviQI GTAC 1 cut(s) 28
DpnI GATC 3 cut(s) 157, 221, 325
DpnII GATC 3 cut(s) 155, 219, 323
DraIII CACNNNGTG 2 cut(s) 94, 485
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 493
Eco72I CACGTG 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 472
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 5 cut(s) 254, 278, 403, 432, 536
FatI CATG 5 cut(s) 250, 274, 399, 428, 532
FauI CCCGC 3 cut(s) 155, 161, 399
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 3 cut(s) 23, 479, 501
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 451
Hin1II CATG 5 cut(s) 254, 278, 403, 432, 536
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 2 cut(s) 80, 376
HphI GGTGA 2 cut(s) 349, 509
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 2 cut(s) 79, 328
Hpy188III TCNNGA 2 cut(s) 293, 380
Hpy8I GTNNAC 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 201, 328, 409
HpyCH4III ACNGT 2 cut(s) 122, 181
HpyCH4IV ACGT 4 cut(s) 43, 90, 451, 481
HpyCH4V TGCA 3 cut(s) 432, 470, 536
HpySE526I ACGT 4 cut(s) 43, 90, 451, 481
Hsp92I GRCGYC 1 cut(s) 451
Hsp92II CATG 5 cut(s) 254, 278, 403, 432, 536
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 3 cut(s) 155, 219, 323
LmnI GCTCC 2 cut(s) 80, 247
LpnPI CCDG 4 cut(s) 8, 22, 278, 393
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 3 cut(s) 457, 479, 479
MaeII ACGT 4 cut(s) 43, 90, 451, 481
MaeIII GTNAC 1 cut(s) 452
MalI GATC 3 cut(s) 157, 221, 325
MboI GATC 3 cut(s) 155, 219, 323
MboII GAAGA 1 cut(s) 118
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 2 cut(s) 155, 323
MhlI GDGCHC 2 cut(s) 274, 517
MluCI AATT 6 cut(s) 172, 296, 312, 393, 523, 573
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 6 cut(s) 117, 176, 213, 261, 390, 522
MslI CAYNNNNRTG 2 cut(s) 279, 461
MspA1I CMGCKG 1 cut(s) 8
MunI CAATTG 1 cut(s) 312
MvnI CGCG 4 cut(s) 20, 150, 168, 200
NdeII GATC 3 cut(s) 155, 219, 323
NlaIII CATG 5 cut(s) 254, 278, 403, 432, 536
NlaIV GGNNCC 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 452
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 3 cut(s) 254, 278, 536
PaeI GCATGC 1 cut(s) 536
PciI ACATGT 1 cut(s) 274
PfeI GAWTC 2 cut(s) 80, 376
PflMI CCANNNNNTGG 1 cut(s) 485
PkrI GCNGC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 91
PmlI CACGTG 1 cut(s) 91
Ppu21I YACGTR 1 cut(s) 91
PscI ACATGT 1 cut(s) 274
PsiI TTATAA 1 cut(s) 62
PspCI CACGTG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 76
PsuI RGATCY 2 cut(s) 155, 323
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 2 cut(s) 279, 461
SaqAI TTAA 6 cut(s) 117, 176, 213, 261, 390, 522
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 155, 219, 323
SduI GDGCHC 2 cut(s) 274, 517
SetI ASST 9 cut(s) 46, 93, 228, 339, 439, 444, 454, 484, 499
SfaNI GCATC 3 cut(s) 457, 479, 479
SmiMI CAYNNNNRTG 2 cut(s) 279, 461
SphI GCATGC 1 cut(s) 536
Sse9I AATT 6 cut(s) 172, 296, 312, 393, 523, 573
SsiI CCGC 6 cut(s) 8, 18, 148, 168, 200, 406
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 2 cut(s) 122, 181
TaiI ACGT 4 cut(s) 46, 93, 454, 484
TaqI TCGA 1 cut(s) 222
TasI AATT 6 cut(s) 172, 296, 312, 393, 523, 573
TfiI GAWTC 2 cut(s) 80, 376
Tru1I TTAA 6 cut(s) 117, 176, 213, 261, 390, 522
Tru9I TTAA 6 cut(s) 117, 176, 213, 261, 390, 522
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 4 cut(s) 246, 347, 388, 428
TspGWI ACGGA 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 485
XapI RAATTY 2 cut(s) 172, 393
XceI RCATGY 3 cut(s) 254, 278, 536
ZraI GACGTC 1 cut(s) 452
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.