Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0003081

Physical Location & Seq

Genomic Coordinates Reverse (-)

61453470 .. 61456804

Gene Structure 3335 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ51390

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGACATGGGGTTGCTATATTTTCTTCTTGCTGATTTGTGCTTCTCTTTCTCAAGAAAATGTCAAGACAAAGCATCCACAGTTGCTGTATGAGTCGAAGTTTATAAAATACTACAGGGAGGAAGTAATAGCCTTCACCACTATCCTTTTTTGCTTTTTCTCTGGAATTCCAAATGTGCGATGGTTTGGCACCGAAGGAGACTACAACGTTCTTGTGATGGATTTATTGGGACCTAGTCTTGAGGATTTATTCAACTTTTGCAGTAGGAAGTTGTCTCTTAAGACTGTTCTCATGCTTGCAGATCAAATGATAAATCGAGTGGAGTTTGTTCATTCCAAGTCTTTTCTACATCGGGATATCAAACCAGACAAGTTCCTAATGGGATTGGGTCGTCGGGCAAATCAGATTTACATCATTGACTTCGGCCTCGCTAAGAAGTATAGAGACACATCAACCCATCAGCGTATTCCTTATAGAGATAATAAGAATTTGACTGGGACAGCAAGATATGCAAGCATGAATACTCATCTTGGCATTGAACAAAGCCGAAGGGATGATCTCGAGTCACTTGGATATGTGCTTATGTACTTCCTAAGAGGAAGTCTTCCATGGCAGGGATTGAAGGCTGGAACTAAAAAGCAGAAGTATGAGAAGATCAGTGAAAAGAAAGTGTCAACATCAATTGAGGTTTTATGCCGTGGTTATCCCACCGAATTTGCCTCATACTTCCATTACTGTAGATCTCTACGTTTTGATGATAAACCAGATTATGCTTATCTGAAGAGATTGTTTCGTGACCTTTTTATTTGCGAAGGTTTTCAGTTTGATTATGTCTTTGATTGGACGATCTTGAAATATCAGCAGTCGAAGATTGCCACACCTCCTACTCGTGCTCCTGGTGGTGCTGGACCAAGCTCTGGACTGCCTCCTGTTGTTGCAAATGTTGAAAGACAATCAGGTGGGGAGGAAGTTAGACCTACTGGTTGGTCGTTGGCAGATCCCTCACGTAGGAGAAATTCTGGACCGATTGTAAATCCTGGAAGCTTGTCTAAACAAAAAAGTCCTGTTGCAAATGATCCATCTGTGTCTAAAGACGCAATGTTATCAAGTTCTAGTTTTTTGCGGTCAACTGGATCATCAAGGAGGGCTGTAGTTTCTAGCAGTCGGGATGCAGTAATTGTTGGCAGTGAATCCGAGCCCTCTCGCCCTCAAACCACAGAGGCAAGTCCTGGAACACTAGGTAAAATTTCTTCTGTCCAAAGAAGTTCACCAGTTGTATCTTCAGAGCAGAATCGTACCACTTCTGGTAGAAATATTTCGACCGTAAAAAACTTAAGTCCACCCTCAGAGGTATTGAGACCCTTCATTTTAATAGCGACGAGAGAGTACAGTATTAGCAGTCGCTTCTCTCAATATGTCGCCCACTGTAAAATTGAAAAACGGTATACAGTTCTCACACACTTCAGGTGGAATTTGTTTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04011 ko04392 map04011 map04392

Pfam Domains

Protein Families

Pkinase

Protein Analysis

494

Amino Acids

56.08

Weight (kDa)

9.62

Isoelectric Point (pI)

53.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	65 - 218	6.8e-16	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	68 - 223	4.1e-07	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	101 - 197	8.6e-07	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003081)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72710
fragaria_vesca	FvH4_6g34010 FvH4_6g34010 FvH4_6g34010
malus_domestica	MD09G1190100.v1.1 MD17G1173000.v1.1
prunus_persica	Prupe.3G046000_v2.0.a1 Prupe.3G046000_v2.0.a1
pyrus_communis	pycom09g10660 pycom17g17150
rosa_chinensis	RchiOBHm_Chr2g0143801 RchiOBHm_Chr2g0143831 RchiOBHm_Chr2g0143921 RchiOBHm_Chr2g0143961 RchiOBHm_Chr2g0144121 RchiOBHm_Chr2g0144141 RchiOBHm_Chr2g0144191 RchiOBHm_Chr2g0144221 RchiOBHm_Chr2g0144271
rosa_laevigata	RLG00000020070
rosa_multiflora	Rmu_sc0002354.1_g000001 Rmu_sc0012360.1_g000001
rosa_roxburghii	Rroxscaffold_2G00101420
rosa_rugosa	Rorug02G0385600 Rorug02G0385700
rosa_samantha	Rh2AG438100 Rh2BG446500 Rh2BG448300 Rh2CG424700 Rh2DG455900 Rh2DG456800 Rh2DG457800
rosa_wichuraiana	Rw2G033290 Rw2G033320 Rw2G035780 Rw2G035830 Rw2G036150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	104
AccB1I	GGYRCC	1 cut(s)	188
AccB7I	CCANNNNNTGG	1 cut(s)	917
AccI	GTMKAC	1 cut(s)	1445
AciI	CCGC	1 cut(s)	1123
AclI	AACGTT	1 cut(s)	207
AclWI	GGATC	3 cut(s)	992, 1070, 1141
AcsI	RAATTY	6 cut(s)	165, 487, 713, 1015, 1245, 1471
AcuI	CTGAAG	3 cut(s)	800, 1266, 1447
AfaI	GTAC	3 cut(s)	587, 1297, 1388
AfiI	CCNNNNNNNGG	3 cut(s)	614, 917, 1008
AflII	CTTAAG	2 cut(s)	278, 1333
AgsI	TTSAA	6 cut(s)	253, 539, 622, 853, 947, 1436
AjnI	CCWGG	3 cut(s)	895, 1036, 1228
AluBI	AGCT	2 cut(s)	915, 1044
AluI	AGCT	2 cut(s)	915, 1044
Alw21I	GWGCWC	1 cut(s)	895
Alw26I	GTCTC	4 cut(s)	192, 279, 438, 1351
AlwI	GGATC	3 cut(s)	992, 1070, 1141
AlwNI	CAGNNNCTG	1 cut(s)	85
Ama87I	CYCGRG	1 cut(s)	560
AoxI	GGCC	1 cut(s)	424
ApoI	RAATTY	6 cut(s)	165, 487, 713, 1015, 1245, 1471
Asp700I	GAANNNNTTC	2 cut(s)	816, 1315
AspS9I	GGNCC	3 cut(s)	230, 908, 1022
AsuHPI	GGTGA	2 cut(s)	127, 1260
AvaI	CYCGRG	1 cut(s)	560
AvaII	GGWCC	3 cut(s)	230, 908, 1022
BanI	GGYRCC	1 cut(s)	188
BanII	GRGCYC	1 cut(s)	1200
BarI	GAAGNNNNNNTAC	2 cut(s)	1234, 1266
BauI	CACGAG	1 cut(s)	888
BbsI	GAAGAC	1 cut(s)	596
Bbv12I	GWGCWC	1 cut(s)	895
BccI	CCATC	4 cut(s)	174, 211, 465, 1087
BceAI	ACGGC	1 cut(s)	681
BciT130I	CCWGG	3 cut(s)	897, 1038, 1230
BcoDI	GTCTC	4 cut(s)	192, 279, 438, 1351
BfaI	CTAG	4 cut(s)	234, 1113, 1158, 1238
BfmI	CTRYAG	3 cut(s)	112, 736, 1149
BfrI	CTTAAG	2 cut(s)	278, 1333
BglII	AGATCT	1 cut(s)	740
Bme1390I	CCNGG	3 cut(s)	897, 1038, 1230
Bme18I	GGWCC	3 cut(s)	230, 908, 1022
BmeT110I	CYCGRG	1 cut(s)	560
BmgT120I	GGNCC	3 cut(s)	230, 908, 1022
BmiI	GGNNCC	2 cut(s)	190, 231
BmrFI	CCNGG	3 cut(s)	897, 1038, 1230
BmrI	ACTGGG	1 cut(s)	504
BmsI	GCATC	2 cut(s)	82, 1159
BmuI	ACTGGG	1 cut(s)	504
BpiI	GAAGAC	1 cut(s)	596
BpuEI	CTTGAG	2 cut(s)	36, 260
BsaAI	YACGTR	1 cut(s)	1007
BsaBI	GATNNNNATC	1 cut(s)	410
BsaI	GGTCTC	1 cut(s)	1351
BsaJI	CCNNGG	2 cut(s)	608, 697
BsaXI	ACNNNNNCTCC	2 cut(s)	877, 907
Bsc4I	CCNNNNNNNGG	3 cut(s)	614, 917, 1008
Bse1I	ACTGG	4 cut(s)	499, 985, 1135, 1271
Bse3DI	GCAATG	1 cut(s)	1104
Bse8I	GATNNNNATC	1 cut(s)	410
BseBI	CCWGG	3 cut(s)	897, 1038, 1230
BseDI	CCNNGG	2 cut(s)	608, 697
BseGI	GGATG	3 cut(s)	73, 559, 1174
BseJI	GATNNNNATC	1 cut(s)	410
BseLI	CCNNNNNNNGG	3 cut(s)	614, 917, 1008
BseMI	GCAATG	1 cut(s)	1104
BseMII	CTCAG	1 cut(s)	1359
BseNI	ACTGG	4 cut(s)	499, 985, 1135, 1271
Bsh1285I	CGRYCG	1 cut(s)	1323
BshFI	GGCC	1 cut(s)	426
BshNI	GGYRCC	1 cut(s)	188
BsiEI	CGRYCG	1 cut(s)	1323
BsiHKAI	GWGCWC	1 cut(s)	895
BsiHKCI	CYCGRG	1 cut(s)	560
BslFI	GGGAC	2 cut(s)	243, 511
BslI	CCNNNNNNNGG	3 cut(s)	614, 917, 1008
BsmAI	GTCTC	4 cut(s)	192, 279, 438, 1351
BsmFI	GGGAC	2 cut(s)	243, 511
BsnI	GGCC	1 cut(s)	426
Bso31I	GGTCTC	1 cut(s)	1351
BsoBI	CYCGRG	1 cut(s)	560
Bsp1286I	GDGCHC	2 cut(s)	895, 1200
Bsp143I	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
Bsp19I	CCATGG	1 cut(s)	608
BspACI	CCGC	1 cut(s)	1123
BspANI	GGCC	1 cut(s)	426
BspCNI	CTCAG	1 cut(s)	1358
BspLI	GGNNCC	2 cut(s)	190, 231
BspPI	GGATC	3 cut(s)	992, 1070, 1141
BspT107I	GGYRCC	1 cut(s)	188
BspTI	CTTAAG	2 cut(s)	278, 1333
BspTNI	GGTCTC	1 cut(s)	1351
BsrDI	GCAATG	1 cut(s)	1104
BsrI	ACTGG	4 cut(s)	499, 985, 1135, 1271
BssECI	CCNNGG	2 cut(s)	608, 697
BssMI	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
BssNAI	GTATAC	1 cut(s)	1446
BssSI	CACGAG	1 cut(s)	888
BssT1I	CCWWGG	1 cut(s)	608
Bst1107I	GTATAC	1 cut(s)	1446
Bst2BI	CACGAG	1 cut(s)	888
Bst2UI	CCWGG	3 cut(s)	897, 1038, 1230
Bst4CI	ACNGT	8 cut(s)	81, 286, 737, 1324, 1391, 1427, 1443, 1450
Bst6I	CTCTTC	1 cut(s)	776
BstAFI	CTTAAG	2 cut(s)	278, 1333
BstAPI	GCANNNNNTGC	1 cut(s)	509
BstBAI	YACGTR	1 cut(s)	1007
BstC8I	GCNNGC	2 cut(s)	297, 514
BstDEI	CTNAG	3 cut(s)	432, 593, 1345
BstDSI	CCRYGG	2 cut(s)	608, 697
BstENI	CCTNNNNNAGG	1 cut(s)	1006
BstF5I	GGATG	3 cut(s)	73, 559, 1174
BstKTI	GATC	8 cut(s)	304, 559, 657, 743, 849, 1000, 1078, 1136
BstMAI	GTCTC	4 cut(s)	192, 279, 438, 1351
BstMBI	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
BstMCI	CGRYCG	1 cut(s)	1323
BstMWI	GCNNNNNNNGC	1 cut(s)	509
BstNI	CCWGG	3 cut(s)	897, 1038, 1230
BstSCI	CCNGG	3 cut(s)	895, 1036, 1228
BstSFI	CTRYAG	3 cut(s)	112, 736, 1149
BstV2I	GAAGAC	1 cut(s)	596
BstX2I	RGATCY	2 cut(s)	740, 997
BstYI	RGATCY	2 cut(s)	740, 997
BstZ17I	GTATAC	1 cut(s)	1446
BsuRI	GGCC	1 cut(s)	426
BtgI	CCRYGG	2 cut(s)	608, 697
BtgZI	GCGATG	1 cut(s)	193
BtsCI	GGATG	3 cut(s)	73, 559, 1174
BtsI	GCAGTG	1 cut(s)	1192
BtsIMutI	CAGTG	3 cut(s)	664, 1192, 1423
Cac8I	GCNNGC	2 cut(s)	297, 514
CaiI	CAGNNNCTG	1 cut(s)	85
Cfr13I	GGNCC	3 cut(s)	230, 908, 1022
CseI	GACGC	1 cut(s)	1103
Csp6I	GTAC	3 cut(s)	586, 1296, 1387
CspCI	CAANNNNNGTGG	2 cut(s)	697, 732
CviAII	CATG	4 cut(s)	6, 292, 517, 609
CviJI	RGCY	8 cut(s)	131, 426, 546, 626, 915, 1044, 1148, 1198
CviKI_1	RGCY	8 cut(s)	131, 426, 546, 626, 915, 1044, 1148, 1198
CviQI	GTAC	3 cut(s)	586, 1296, 1387
DdeI	CTNAG	3 cut(s)	432, 593, 1345
DpnI	GATC	8 cut(s)	303, 558, 656, 742, 848, 999, 1077, 1135
DpnII	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
Eam1104I	CTCTTC	1 cut(s)	776
EarI	CTCTTC	1 cut(s)	776
Eco130I	CCWWGG	1 cut(s)	608
Eco24I	GRGCYC	1 cut(s)	1200
Eco31I	GGTCTC	1 cut(s)	1351
Eco32I	GATATC	1 cut(s)	358
Eco47I	GGWCC	3 cut(s)	230, 908, 1022
Eco57I	CTGAAG	3 cut(s)	800, 1266, 1447
Eco88I	CYCGRG	1 cut(s)	560
EcoNI	CCTNNNNNAGG	1 cut(s)	1006
EcoO109I	RGGNCCY	1 cut(s)	230
EcoRI	GAATTC	1 cut(s)	165
EcoRII	CCWGG	3 cut(s)	895, 1036, 1228
EcoRV	GATATC	1 cut(s)	358
EcoT14I	CCWWGG	1 cut(s)	608
EcoT38I	GRGCYC	1 cut(s)	1200
ErhI	CCWWGG	1 cut(s)	608
FaeI	CATG	4 cut(s)	9, 295, 520, 612
FaqI	GGGAC	2 cut(s)	243, 511
FatI	CATG	4 cut(s)	5, 291, 516, 608
FblI	GTMKAC	1 cut(s)	1445
FokI	GGATG	3 cut(s)	60, 566, 1181
FriOI	GRGCYC	1 cut(s)	1200
FspBI	CTAG	4 cut(s)	234, 1113, 1158, 1238
HaeIII	GGCC	1 cut(s)	426
HgaI	GACGC	1 cut(s)	1103
Hin1II	CATG	4 cut(s)	9, 295, 520, 612
HincII	GTYRAC	2 cut(s)	675, 1128
HindII	GTYRAC	2 cut(s)	675, 1128
HindIII	AAGCTT	1 cut(s)	1042
HinfI	GANTC	4 cut(s)	92, 563, 1190, 1291
HphI	GGTGA	2 cut(s)	127, 1260
Hpy166II	GTNNAC	5 cut(s)	675, 1128, 1268, 1340, 1446
Hpy188I	TCNGA	5 cut(s)	405, 780, 1195, 1285, 1348
Hpy8I	GTNNAC	5 cut(s)	675, 1128, 1268, 1340, 1446
Hpy99I	CGWCG	2 cut(s)	396, 1381
HpyAV	CCTTC	6 cut(s)	142, 188, 543, 616, 806, 1372
HpyCH4III	ACNGT	8 cut(s)	81, 286, 737, 1324, 1391, 1427, 1443, 1450
HpyCH4IV	ACGT	3 cut(s)	207, 748, 1006
HpyCH4V	TGCA	6 cut(s)	261, 299, 512, 938, 1070, 1172
HpyF10VI	GCNNNNNNNGC	1 cut(s)	509
HpyF3I	CTNAG	3 cut(s)	432, 593, 1345
HpySE526I	ACGT	3 cut(s)	207, 748, 1006
Hsp92II	CATG	4 cut(s)	9, 295, 520, 612
Kzo9I	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
LmnI	GCTCC	1 cut(s)	898
LweI	GCATC	2 cut(s)	82, 1159
MaeI	CTAG	4 cut(s)	234, 1113, 1158, 1238
MaeII	ACGT	3 cut(s)	207, 748, 1006
MaeIII	GTNAC	2 cut(s)	564, 794
MalI	GATC	8 cut(s)	303, 558, 656, 742, 848, 999, 1077, 1135
MboI	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
MboII	GAAGA	7 cut(s)	16, 596, 664, 793, 880, 1242, 1272
MfeI	CAATTG	1 cut(s)	681
MflI	RGATCY	2 cut(s)	740, 997
MhlI	GDGCHC	2 cut(s)	895, 1200
MluCI	AATT	9 cut(s)	165, 487, 681, 713, 1015, 1176, 1245, 1431, 1471
MlyI	GAGTC	2 cut(s)	101, 572
MroXI	GAANNNNTTC	2 cut(s)	816, 1315
MseI	TTAA	4 cut(s)	279, 1334, 1370, 1483
MspCI	CTTAAG	2 cut(s)	278, 1333
MspR9I	CCNGG	3 cut(s)	897, 1038, 1230
MunI	CAATTG	1 cut(s)	681
MvaI	CCWGG	3 cut(s)	897, 1038, 1230
MwoI	GCNNNNNNNGC	1 cut(s)	509
NcoI	CCATGG	1 cut(s)	608
NdeII	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
NlaIII	CATG	4 cut(s)	9, 295, 520, 612
NlaIV	GGNNCC	2 cut(s)	190, 231
NmuCI	GTSAC	2 cut(s)	564, 794
PaeR7I	CTCGAG	1 cut(s)	560
PdmI	GAANNNNTTC	2 cut(s)	816, 1315
PfeI	GAWTC	2 cut(s)	1190, 1291
PflFI	GACNNNGTC	1 cut(s)	234
PflMI	CCANNNNNTGG	1 cut(s)	917
PfoI	TCCNGGA	2 cut(s)	1036, 1228
PleI	GAGTC	2 cut(s)	100, 571
PpsI	GAGTC	2 cut(s)	100, 571
Ppu21I	YACGTR	1 cut(s)	1007
PpuMI	RGGWCCY	1 cut(s)	230
PsiI	TTATAA	1 cut(s)	104
Psp1406I	AACGTT	1 cut(s)	207
Psp5II	RGGWCCY	1 cut(s)	230
Psp6I	CCWGG	3 cut(s)	895, 1036, 1228
PspGI	CCWGG	3 cut(s)	895, 1036, 1228
PspN4I	GGNNCC	2 cut(s)	190, 231
PspPI	GGNCC	3 cut(s)	230, 908, 1022
PspPPI	RGGWCCY	1 cut(s)	230
PstNI	CAGNNNCTG	1 cut(s)	85
PsuI	RGATCY	2 cut(s)	740, 997
PsyI	GACNNNGTC	1 cut(s)	234
RsaI	GTAC	3 cut(s)	587, 1297, 1388
RsaNI	GTAC	3 cut(s)	586, 1296, 1387
SaqAI	TTAA	4 cut(s)	279, 1334, 1370, 1483
Sau3AI	GATC	8 cut(s)	301, 556, 654, 740, 846, 997, 1075, 1133
Sau96I	GGNCC	3 cut(s)	230, 908, 1022
SchI	GAGTC	2 cut(s)	101, 572
ScrFI	CCNGG	3 cut(s)	897, 1038, 1230
SduI	GDGCHC	2 cut(s)	895, 1200
SfaNI	GCATC	2 cut(s)	82, 1159
SfcI	CTRYAG	3 cut(s)	112, 736, 1149
Sfr274I	CTCGAG	1 cut(s)	560
SinI	GGWCC	3 cut(s)	230, 908, 1022
SlaI	CTCGAG	1 cut(s)	560
SmlI	CTYRAG	5 cut(s)	51, 239, 278, 560, 1333
SmoI	CTYRAG	5 cut(s)	51, 239, 278, 560, 1333
Sse9I	AATT	9 cut(s)	165, 487, 681, 713, 1015, 1176, 1245, 1431, 1471
SsiI	CCGC	1 cut(s)	1123
SspI	AATATT	1 cut(s)	1315
SspMI	CTAG	4 cut(s)	234, 1113, 1158, 1238
StyD4I	CCNGG	3 cut(s)	895, 1036, 1228
StyI	CCWWGG	1 cut(s)	608
TaaI	ACNGT	8 cut(s)	81, 286, 737, 1324, 1391, 1427, 1443, 1450
TaiI	ACGT	3 cut(s)	210, 751, 1009
TaqI	TCGA	5 cut(s)	95, 316, 561, 866, 1319
TaqII	GACCGA	1 cut(s)	1039
TasI	AATT	9 cut(s)	165, 487, 681, 713, 1015, 1176, 1245, 1431, 1471
TatI	WGTACW	2 cut(s)	585, 1386
TfiI	GAWTC	2 cut(s)	1190, 1291
Tru1I	TTAA	4 cut(s)	279, 1334, 1370, 1483
Tru9I	TTAA	4 cut(s)	279, 1334, 1370, 1483
TscAI	CASTG	3 cut(s)	664, 1192, 1430
TseFI	GTSAC	2 cut(s)	564, 794
Tsp45I	GTSAC	2 cut(s)	564, 794
TspDTI	ATGAA	3 cut(s)	320, 533, 1354
TspRI	CASTG	3 cut(s)	664, 1192, 1430
Tth111I	GACNNNGTC	1 cut(s)	234
Van91I	CCANNNNNTGG	1 cut(s)	917
Vha464I	CTTAAG	2 cut(s)	278, 1333
VpaK11BI	GGWCC	3 cut(s)	230, 908, 1022
XagI	CCTNNNNNAGG	1 cut(s)	1006
XapI	RAATTY	6 cut(s)	165, 487, 713, 1015, 1245, 1471
XcmI	CCANNNNNNNNNTGG	1 cut(s)	177
XhoI	CTCGAG	1 cut(s)	560
XmiI	GTMKAC	1 cut(s)	1445
XmnI	GAANNNNTTC	2 cut(s)	816, 1315
XspI	CTAG	4 cut(s)	234, 1113, 1158, 1238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.