Rw2G033290
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003081
Physical Location & Seq
Reverse (-)
54545836 .. 54548181
2346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033290.1

Sequence Viewer

Length: 702 bp
ATGGTGGCATATCATCATCATCAAGAAAATGTCAAGACAAAGCATCAACAGTTGTTGTATGAGTCGAAGTTGTATAAAATACTACAGGGAGGGACTGGAATTCCAAATGTGCGATGGTTTGGCACCGAAGGAGACTACAATGTTCTTGTGATGGATTTATTGGGACCTAGTCTTGAGGATTTATTCAACTTTTGCAGTAGGAAGTTGTCTCTTAAGACTGTTGCAGATCAAATGATAAATCGAGTGGAGTTTGTTCATTCCAAGTCTTTTCTACATCGGGATATCAAACCAGACAAGTTCCTAATGGGATTGGGTCGTCGGGCGAATCAGATTTACATCATTGACTTTGGCCTCGCTAAGAAGTATAGAGACACATCAACCCATCAGCATATTCCTTATAGAGATAATAAGAATTTGACTGGGACAGCAAGATATGCAAGCATGAATACTCATCTTGGCATTGAACAAAGCCGAAGGGATGATCTCGAGTCACTTGGATATGTGCTTATGTACTTCCTAAGAGGAAGTCTTCCATGGCAGGGATTGAAGGCTGGAACTAAAAAGCAGAAGTATGAGAAGATCAGTGAAAAGAAAGTGTCAACATCAATTGAGGACTCAGAAGATATATGTGCGTTGCAAGCTGTGCGATTATTGATGGCTCAGGTTAACCATCAAGTTTGTGGTTGCTATAGCTCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

233

Amino Acids

26.78

Weight (kDa)

9.23

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 8 - 196 2.1e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 15 - 193 7.9e-06 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 59 - 172 3.4e-08 Fungal protein kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AcsI RAATTY 2 cut(s) 99, 412
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 1 cut(s) 539
AflII CTTAAG 1 cut(s) 212
AgsI TTSAA 3 cut(s) 187, 464, 547
AluBI AGCT 2 cut(s) 641, 693
AluI AGCT 2 cut(s) 641, 693
Alw26I GTCTC 3 cut(s) 126, 213, 363
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 1 cut(s) 349
ApoI RAATTY 2 cut(s) 99, 412
AspS9I GGNCC 1 cut(s) 164
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 521
BccI CCATC 5 cut(s) 108, 145, 390, 649, 678
BcoDI GTCTC 3 cut(s) 126, 213, 363
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 83, 688
BfrI CTTAAG 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 694
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 164
BmiI GGNNCC 2 cut(s) 124, 165
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 660
Bpu1102I GCTNAGC 1 cut(s) 694
BpuEI CTTGAG 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 533
Bsc4I CCNNNNNNNGG 1 cut(s) 539
Bse1I ACTGG 2 cut(s) 100, 424
Bse8I GATNNNNATC 1 cut(s) 335
BseDI CCNNGG 1 cut(s) 533
BseGI GGATG 1 cut(s) 484
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 539
BseMII CTCAG 2 cut(s) 630, 674
BseNI ACTGG 2 cut(s) 100, 424
BshFI GGCC 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 122
BsiHKCI CYCGRG 1 cut(s) 485
BslFI GGGAC 3 cut(s) 106, 177, 436
BslI CCNNNNNNNGG 1 cut(s) 539
BsmAI GTCTC 3 cut(s) 126, 213, 363
BsmFI GGGAC 3 cut(s) 106, 177, 436
BsnI GGCC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 485
Bsp143I GATC 3 cut(s) 226, 481, 579
Bsp1720I GCTNAGC 1 cut(s) 694
Bsp19I CCATGG 1 cut(s) 533
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 2 cut(s) 629, 673
BspLI GGNNCC 2 cut(s) 124, 165
BspT107I GGYRCC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 212
BsrI ACTGG 2 cut(s) 100, 424
BssECI CCNNGG 1 cut(s) 533
BssMI GATC 3 cut(s) 226, 481, 579
BssT1I CCWWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 51, 220
BstAFI CTTAAG 1 cut(s) 212
BstAPI GCANNNNNTGC 2 cut(s) 434, 643
BstC8I GCNNGC 2 cut(s) 439, 639
BstDEI CTNAG 5 cut(s) 357, 518, 616, 660, 694
BstDSI CCRYGG 1 cut(s) 533
BstF5I GGATG 1 cut(s) 484
BstKTI GATC 3 cut(s) 229, 484, 582
BstMAI GTCTC 3 cut(s) 126, 213, 363
BstMBI GATC 3 cut(s) 226, 481, 579
BstMWI GCNNNNNNNGC 3 cut(s) 434, 638, 643
BstSFI CTRYAG 2 cut(s) 83, 688
BstV2I GAAGAC 1 cut(s) 521
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 127
BtsCI GGATG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 439, 639
Cfr13I GGNCC 1 cut(s) 164
Csp6I GTAC 1 cut(s) 511
CviAII CATG 2 cut(s) 442, 534
CviJI RGCY 6 cut(s) 351, 471, 551, 641, 659, 693
CviKI_1 RGCY 6 cut(s) 351, 471, 551, 641, 659, 693
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 5 cut(s) 357, 518, 616, 660, 694
DpnI GATC 3 cut(s) 228, 483, 581
DpnII GATC 3 cut(s) 226, 481, 579
Eco130I CCWWGG 1 cut(s) 533
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 99
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 533
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 445, 537
FaqI GGGAC 3 cut(s) 106, 177, 436
FatI CATG 2 cut(s) 441, 533
FokI GGATG 1 cut(s) 491
FspBI CTAG 1 cut(s) 168
HaeIII GGCC 1 cut(s) 351
Hin1II CATG 2 cut(s) 445, 537
HincII GTYRAC 2 cut(s) 600, 667
HindII GTYRAC 2 cut(s) 600, 667
HinfI GANTC 4 cut(s) 62, 325, 488, 614
HpaI GTTAAC 1 cut(s) 667
Hpy166II GTNNAC 2 cut(s) 600, 667
Hpy188I TCNGA 2 cut(s) 330, 619
Hpy188III TCNNGA 5 cut(s) 23, 34, 173, 278, 485
Hpy8I GTNNAC 2 cut(s) 600, 667
Hpy99I CGWCG 1 cut(s) 321
HpyAV CCTTC 3 cut(s) 122, 468, 541
HpyCH4III ACNGT 2 cut(s) 51, 220
HpyCH4V TGCA 4 cut(s) 195, 224, 437, 637
HpyF10VI GCNNNNNNNGC 3 cut(s) 434, 638, 643
HpyF3I CTNAG 5 cut(s) 357, 518, 616, 660, 694
Hsp92II CATG 2 cut(s) 445, 537
KspAI GTTAAC 1 cut(s) 667
Kzo9I GATC 3 cut(s) 226, 481, 579
LpnPI CCDG 7 cut(s) 71, 81, 303, 405, 524, 537, 647
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 168
MaeIII GTNAC 1 cut(s) 489
MalI GATC 3 cut(s) 228, 483, 581
MboI GATC 3 cut(s) 226, 481, 579
MboII GAAGA 3 cut(s) 521, 589, 632
MfeI CAATTG 1 cut(s) 606
MluCI AATT 3 cut(s) 99, 412, 606
MlyI GAGTC 3 cut(s) 71, 497, 608
MnlI CCTC 5 cut(s) 83, 169, 362, 515, 604
MseI TTAA 2 cut(s) 213, 666
MspCI CTTAAG 1 cut(s) 212
MunI CAATTG 1 cut(s) 606
MwoI GCNNNNNNNGC 3 cut(s) 434, 638, 643
NcoI CCATGG 1 cut(s) 533
NdeII GATC 3 cut(s) 226, 481, 579
NlaIII CATG 2 cut(s) 445, 537
NlaIV GGNNCC 2 cut(s) 124, 165
NmuCI GTSAC 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 485
PfeI GAWTC 1 cut(s) 325
PflFI GACNNNGTC 1 cut(s) 168
PleI GAGTC 3 cut(s) 70, 496, 608
PpsI GAGTC 3 cut(s) 70, 496, 608
PpuMI RGGWCCY 1 cut(s) 164
Psp5II RGGWCCY 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 124, 165
PspPI GGNCC 1 cut(s) 164
PspPPI RGGWCCY 1 cut(s) 164
PsyI GACNNNGTC 1 cut(s) 168
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
SaqAI TTAA 2 cut(s) 213, 666
Sau3AI GATC 3 cut(s) 226, 481, 579
Sau96I GGNCC 1 cut(s) 164
SchI GAGTC 3 cut(s) 71, 497, 608
SetI ASST 4 cut(s) 169, 643, 666, 695
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 2 cut(s) 83, 688
Sfr274I CTCGAG 1 cut(s) 485
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 485
SmlI CTYRAG 3 cut(s) 173, 212, 485
SmoI CTYRAG 3 cut(s) 173, 212, 485
Sse9I AATT 3 cut(s) 99, 412, 606
SspMI CTAG 1 cut(s) 168
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 2 cut(s) 51, 220
TaqI TCGA 3 cut(s) 65, 241, 486
TasI AATT 3 cut(s) 99, 412, 606
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 325
Tru1I TTAA 2 cut(s) 213, 666
Tru9I TTAA 2 cut(s) 213, 666
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 1 cut(s) 489
Tsp45I GTSAC 1 cut(s) 489
TspDTI ATGAA 2 cut(s) 245, 458
TspRI CASTG 1 cut(s) 589
Tth111I GACNNNGTC 1 cut(s) 168
Vha464I CTTAAG 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 164
XapI RAATTY 2 cut(s) 99, 412
XcmI CCANNNNNNNNNTGG 2 cut(s) 111, 677
XhoI CTCGAG 1 cut(s) 485
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.