Rw2G033320
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003081
Physical Location & Seq
Reverse (-)
54596943 .. 54598745
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033320.1

Sequence Viewer

Length: 879 bp
ATGAATACTCATCTTGGCATTGAACAAAGCCGAAGGGATGATCTCGAGTCACTTGGATATGTGCTTATGTACTTCCTAAGAGGAAGTCTTCCATGGCAGGGATTGAAGGCTGGAACTAAAAAGCAGAAGTATGAGAAGATCAGTGAAAAGAAAGTGTCAACATCAATTGAGGCCTTATGCCGTGGTTATCCCACCGAATTTGCCTCATACTTCCATTACTGTAGATCTCTACGTTTTGATGATAAACCAGATTATGCTTATCTGAAGAGATTGTTTCGTGACCTTTTTATTCGCGAAGGTTTTCAGTTTGATTATGTCTTTGATTGGATGATCTTGAAATATCAGCAGTCGCAGATTGCCACACCTCCTACTCGTGCTCCTGGTGGTGCTGGACCAAGCTCTGGACTGCCTCCAGTTGTTGCAAATGTTGAAAGACAATCAGGTGGGGAGGAAGTTAGACCTACTGGTTGGTCGTTGGCAGATCCCTCACGTAGGAGAAATTCTGGACCGATTGTAAATCCTGGAAGCTTGTCTAAACAAAAAAGTCCTGTTGCAAATGATCCATCTGTGTCTAAAGATGCAATGTTATCAAGTTCTAGTTTTTTGCGGTCAACTGGATCATCAAGGAGGGCTGTAGTTTCTAGCAGTCGGGATGCAGTAATTGTTGGCAGTGAATCCGAGCCCTCTCGCCCTCAAACCACAGAGGCAAGTCCTGGAACACTAGGTAAAATTTCTTCTGTCCAAAGTTCACCTGTTGTATCTTCAGAGCAGAATCGTACCACTTCTGGTAGAAATATTTCGGCCGTAAAAAACTTAGAGTCCACCCTCAGAGGTATTGAGACCCTTCATTTTAATAGCGACGAGAGAGTACAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

292

Amino Acids

32.32

Weight (kDa)

9.51

Isoelectric Point (pI)

72.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 401
AccII CGCG 1 cut(s) 294
AciI CCGC 1 cut(s) 607
AclWI GGATC 3 cut(s) 476, 554, 625
AcoI YGGCCR 1 cut(s) 801
AcsI RAATTY 3 cut(s) 197, 499, 729
AcuI CTGAAG 2 cut(s) 284, 747
AfaI GTAC 3 cut(s) 71, 778, 870
AfiI CCNNNNNNNGG 3 cut(s) 98, 401, 492
AgsI TTSAA 4 cut(s) 23, 106, 337, 431
AjnI CCWGG 3 cut(s) 379, 520, 712
AluBI AGCT 2 cut(s) 399, 528
AluI AGCT 2 cut(s) 399, 528
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 1 cut(s) 833
AlwI GGATC 3 cut(s) 476, 554, 625
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 2 cut(s) 171, 801
ApoI RAATTY 3 cut(s) 197, 499, 729
Asp700I GAANNNNTTC 2 cut(s) 300, 796
AspS9I GGNCC 2 cut(s) 392, 506
AsuHPI GGTGA 1 cut(s) 741
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 2 cut(s) 392, 506
BanII GRGCYC 1 cut(s) 684
BarI GAAGNNNNNNTAC 2 cut(s) 718, 750
BauI CACGAG 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 379
BccI CCATC 1 cut(s) 571
BceAI ACGGC 2 cut(s) 165, 788
BciT130I CCWGG 3 cut(s) 381, 522, 714
BcoDI GTCTC 1 cut(s) 833
BfaI CTAG 3 cut(s) 597, 642, 722
BfmI CTRYAG 2 cut(s) 220, 633
BglII AGATCT 1 cut(s) 224
Bme1390I CCNGG 3 cut(s) 381, 522, 714
Bme18I GGWCC 2 cut(s) 392, 506
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 392, 506
BmrFI CCNGG 3 cut(s) 381, 522, 714
BmsI GCATC 2 cut(s) 568, 643
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 396
BsaAI YACGTR 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 2 cut(s) 92, 181
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 401, 492
Bse1I ACTGG 3 cut(s) 413, 469, 619
Bse3DI GCAATG 1 cut(s) 588
BseBI CCWGG 3 cut(s) 381, 522, 714
BseDI CCNNGG 2 cut(s) 92, 181
BseGI GGATG 3 cut(s) 43, 333, 658
BseLI CCNNNNNNNGG 3 cut(s) 98, 401, 492
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 1 cut(s) 841
BseNI ACTGG 3 cut(s) 413, 469, 619
BseX3I CGGCCG 1 cut(s) 801
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 804
BshFI GGCC 2 cut(s) 173, 803
BsiEI CGRYCG 1 cut(s) 804
BsiHKAI GWGCWC 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 44
BslI CCNNNNNNNGG 3 cut(s) 98, 401, 492
BsmAI GTCTC 1 cut(s) 833
BsnI GGCC 2 cut(s) 173, 803
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 379, 684
Bsp143I GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
Bsp19I CCATGG 1 cut(s) 92
Bsp68I TCGCGA 1 cut(s) 294
BspACI CCGC 1 cut(s) 607
BspANI GGCC 2 cut(s) 173, 803
BspCNI CTCAG 1 cut(s) 840
BspFNI CGCG 1 cut(s) 294
BspPI GGATC 3 cut(s) 476, 554, 625
BspTNI GGTCTC 1 cut(s) 833
BsrDI GCAATG 1 cut(s) 588
BsrI ACTGG 3 cut(s) 413, 469, 619
BssECI CCNNGG 2 cut(s) 92, 181
BssMI GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
BssSI CACGAG 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 92
Bst2BI CACGAG 1 cut(s) 372
Bst2UI CCWGG 3 cut(s) 381, 522, 714
Bst4CI ACNGT 2 cut(s) 221, 873
Bst6I CTCTTC 1 cut(s) 260
BstBAI YACGTR 1 cut(s) 491
BstDEI CTNAG 3 cut(s) 77, 814, 827
BstDSI CCRYGG 2 cut(s) 92, 181
BstENI CCTNNNNNAGG 1 cut(s) 490
BstF5I GGATG 3 cut(s) 43, 333, 658
BstFNI CGCG 1 cut(s) 294
BstKTI GATC 7 cut(s) 43, 141, 227, 333, 484, 562, 620
BstMAI GTCTC 1 cut(s) 833
BstMBI GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
BstMCI CGRYCG 1 cut(s) 804
BstNI CCWGG 3 cut(s) 381, 522, 714
BstSCI CCNGG 3 cut(s) 379, 520, 712
BstSFI CTRYAG 2 cut(s) 220, 633
BstUI CGCG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 2 cut(s) 224, 481
BstYI RGATCY 2 cut(s) 224, 481
BstZI CGGCCG 1 cut(s) 801
BsuRI GGCC 2 cut(s) 173, 803
BtgI CCRYGG 2 cut(s) 92, 181
BtsCI GGATG 3 cut(s) 43, 333, 658
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 2 cut(s) 148, 676
BtuMI TCGCGA 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 392, 506
Csp6I GTAC 3 cut(s) 70, 777, 869
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 1 cut(s) 93
CviJI RGCY 8 cut(s) 30, 110, 173, 399, 528, 632, 682, 803
CviKI_1 RGCY 8 cut(s) 30, 110, 173, 399, 528, 632, 682, 803
CviQI GTAC 3 cut(s) 70, 777, 869
DdeI CTNAG 3 cut(s) 77, 814, 827
DpnI GATC 7 cut(s) 42, 140, 226, 332, 483, 561, 619
DpnII GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
EaeI YGGCCR 1 cut(s) 801
EagI CGGCCG 1 cut(s) 801
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EclXI CGGCCG 1 cut(s) 801
Eco130I CCWWGG 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 684
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 2 cut(s) 392, 506
Eco52I CGGCCG 1 cut(s) 801
Eco57I CTGAAG 2 cut(s) 284, 747
Eco88I CYCGRG 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 379, 520, 712
EcoT14I CCWWGG 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 1 cut(s) 92
FaeI CATG 1 cut(s) 96
FaiI YATR 8 cut(s) 60, 68, 94, 132, 178, 208, 255, 315
FatI CATG 1 cut(s) 92
FokI GGATG 3 cut(s) 50, 340, 665
FriOI GRGCYC 1 cut(s) 684
FspBI CTAG 3 cut(s) 597, 642, 722
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 2 cut(s) 173, 803
Hin1II CATG 1 cut(s) 96
HincII GTYRAC 2 cut(s) 159, 612
HindII GTYRAC 2 cut(s) 159, 612
HindIII AAGCTT 1 cut(s) 526
HinfI GANTC 4 cut(s) 47, 674, 772, 818
HphI GGTGA 1 cut(s) 741
Hpy166II GTNNAC 4 cut(s) 159, 612, 749, 822
Hpy188I TCNGA 4 cut(s) 264, 679, 766, 830
Hpy188III TCNNGA 7 cut(s) 44, 278, 293, 334, 402, 504, 650
Hpy8I GTNNAC 4 cut(s) 159, 612, 749, 822
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 4 cut(s) 27, 100, 290, 854
HpyCH4III ACNGT 2 cut(s) 221, 873
HpyCH4IV ACGT 2 cut(s) 232, 490
HpyCH4V TGCA 4 cut(s) 422, 554, 581, 656
HpyF3I CTNAG 3 cut(s) 77, 814, 827
HpySE526I ACGT 2 cut(s) 232, 490
Hsp92II CATG 1 cut(s) 96
Kzo9I GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
LmnI GCTCC 1 cut(s) 382
LweI GCATC 2 cut(s) 568, 643
MaeI CTAG 3 cut(s) 597, 642, 722
MaeII ACGT 2 cut(s) 232, 490
MaeIII GTNAC 2 cut(s) 48, 278
MalI GATC 7 cut(s) 42, 140, 226, 332, 483, 561, 619
MboI GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
MboII GAAGA 5 cut(s) 80, 148, 277, 726, 753
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 2 cut(s) 224, 481
MhlI GDGCHC 2 cut(s) 379, 684
MluCI AATT 5 cut(s) 165, 197, 499, 660, 729
MlyI GAGTC 2 cut(s) 56, 827
MroXI GAANNNNTTC 2 cut(s) 300, 796
MseI TTAA 1 cut(s) 852
MspR9I CCNGG 3 cut(s) 381, 522, 714
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 3 cut(s) 381, 522, 714
MvnI CGCG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 92
NdeII GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
NlaIII CATG 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 48, 278
NruI TCGCGA 1 cut(s) 294
PaeR7I CTCGAG 1 cut(s) 44
PceI AGGCCT 1 cut(s) 173
PdmI GAANNNNTTC 2 cut(s) 300, 796
PfeI GAWTC 2 cut(s) 674, 772
PflMI CCANNNNNTGG 1 cut(s) 401
PfoI TCCNGGA 2 cut(s) 520, 712
PleI GAGTC 2 cut(s) 55, 826
PpsI GAGTC 2 cut(s) 55, 826
Ppu21I YACGTR 1 cut(s) 491
Psp6I CCWGG 3 cut(s) 379, 520, 712
PspGI CCWGG 3 cut(s) 379, 520, 712
PspPI GGNCC 2 cut(s) 392, 506
PsuI RGATCY 2 cut(s) 224, 481
RruI TCGCGA 1 cut(s) 294
RsaI GTAC 3 cut(s) 71, 778, 870
RsaNI GTAC 3 cut(s) 70, 777, 869
SaqAI TTAA 1 cut(s) 852
Sau3AI GATC 7 cut(s) 40, 138, 224, 330, 481, 559, 617
Sau96I GGNCC 2 cut(s) 392, 506
SchI GAGTC 2 cut(s) 56, 827
ScrFI CCNGG 3 cut(s) 381, 522, 714
SduI GDGCHC 2 cut(s) 379, 684
SfaNI GCATC 2 cut(s) 568, 643
SfcI CTRYAG 2 cut(s) 220, 633
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 2 cut(s) 392, 506
SlaI CTCGAG 1 cut(s) 44
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 165, 197, 499, 660, 729
SseBI AGGCCT 1 cut(s) 173
SsiI CCGC 1 cut(s) 607
SspI AATATT 1 cut(s) 796
SspMI CTAG 3 cut(s) 597, 642, 722
StuI AGGCCT 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 379, 520, 712
StyI CCWWGG 1 cut(s) 92
TaaI ACNGT 2 cut(s) 221, 873
TaiI ACGT 2 cut(s) 235, 493
TaqI TCGA 1 cut(s) 45
TaqII GACCGA 1 cut(s) 523
TasI AATT 5 cut(s) 165, 197, 499, 660, 729
TatI WGTACW 2 cut(s) 69, 868
TfiI GAWTC 2 cut(s) 674, 772
Tru1I TTAA 1 cut(s) 852
Tru9I TTAA 1 cut(s) 852
TscAI CASTG 2 cut(s) 148, 676
TseFI GTSAC 2 cut(s) 48, 278
Tsp45I GTSAC 2 cut(s) 48, 278
TspDTI ATGAA 2 cut(s) 17, 836
TspRI CASTG 2 cut(s) 148, 676
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 392, 506
XagI CCTNNNNNAGG 1 cut(s) 490
XapI RAATTY 3 cut(s) 197, 499, 729
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 2 cut(s) 300, 796
XspI CTAG 3 cut(s) 597, 642, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.