RchiOBHm_Chr2g0172611

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001832
Physical Location & Seq
Forward (+)
85906503 .. 85914253
7751 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53989

Sequence Viewer

Length: 3162 bp
ATGCTCCGTAACAACTTTGGGACTGCGGAAGAGGGTGAGCCTCCTGACCGGCTTTATATGTTGGCTCAGTGCATGACAGATCTTTCGGTCAATGAATGTAAGACGTGCTATGATTTGAGTAATCATGCCTTGGCTGGTTGCCTTCCTAACACAGGAGGTCGTGCTTTATTTGATGGTTGCTTTATAAGGGCTGAAAATTACAGCTTCTTTGATGAGACATTCCAAGATAAAGATTTCAAGCATTGCAGCGACAATGCCGAGTTCAATCAGAAATTTAACCAAACACCAGAGAAAGTGTTCAAAAAACTTGTGAAGTTGGCTGAGCATAATGATGGATTTGCAGTCGCCAAAGATAGCTCAAATGGTTTTAATGGTTTTACAGTGTTTGCTATGGCTAATTGCTGGAGGAGTCTAGACAGTAGTAAGTGTTCTTCATGTGTAGAAAATGCAGCCAACCGTGCAATTTCATGCTTGCCATCGTCTGAGGGTCGTGTGCTCAATTCTGGCTGCGTTTTACGTTACTCTGATTATTACTTTGGCAATAACCCTCGAGCCCTCGAAATCAAAGCTAAGGGAAAGGAGAGGGAGATGGCAGTTTTTAACCAAAGCTCAGAGTTCCAGAATTTTAAGTACTCAACACTAGTGAAAGCAACCGAGTGCTTCGATGAAGCCCATAAGCTTGGTCAAGGAGCATATGGTGAAGTATTTAAGGGCACTTTACATGATGGTAGAGAAATTGCTATAAAACGTTTGTTCATAACCGGAAAAACCCAAACTCAAGAGGTGTACAATGAAATTCATATCATTAGAAAAGCTCAACATAAGAACTTGGTTCGCTTCCTTGGCTGCTGCTTCACCAACATCAGCTGTTTCCTTATCTACGAATTCCTAGCAAACAGAAGCCTCGACCTTATATTATTTGAACTTCGATGTTTAATTACTAGTACACAGGTGTGGGACCATGTCCAAAATAATACGATTTCTCGAATTATCGATAAGAGTATGAAGATTGAAGACATTGAAGAAATTAAAAGAGTTGTTCAGGTGGGTCTGTTGTGCACTCAAGAATTACCAGAGTTACGTCCTACCATGACATCAGTAATTCGAATGCTTATACGAAGAGACTTGGCATTGCCAGAGCCATCAAAGCCACCTTTCCTTGATATTCAACCTCCACAAATCCAAGGCATGCATACCAAAACCTCAACATGGCTCTTCTTCTTCATCTTCACCTTCTTCTCGCTTTTCTACCTTTTTCTCTGTGATCCCAGAACCTCGGAAGCTGGCCTCTACTGCGGAGCTGCCAAGCCACCACCAAACTCTAGCTTCATTCCAACTTTTGTCGCAGAAATGGAGAGTTTATCAGAGCTCATCACCAAACACCACTGGGGCACCCATTTCTTTAACTCCACGACCCCCATGTACGGCCTAGCGGAATGCTACAACGACCTGTCGCACACTGATTGTCTTCTCTGCTACGCCGCCAGCCGCACCGAGATCCCCCGCTGCCTCCCCTCCCTCTCCGCCCGCATCTTTCTCGACGGCTGCTTCCTCCGCTACGACAACTACAGCTTCTACTCCGAAGCTTCAGTCCCCGCAGTCGACTCCGTCGTCTGCGGCCAAGAAGAGGCGGCGGACGCGGCAGTGTTTAAGGAGAACGTTGCGGAGGTGATTGAGAGGGTTAAGAGGTCCGCGGCGGAGAATGGGGGGTTTGGGGTAGGGGAAGTGGGTGGGGAGGTGTATGCTTTGGGGCAGTGCTGGAAGACGGTGGATGGTGATGGGTGTAGAGAGTGTTTGGAGAAGGGAGGTGAGGCGGTGAGAGGCTGTGGGTTGAAGACAGAAGGCAAGGCTTTGAATGCTGGATGTTACTTGAGGTATTCGACTCGGAAGTTCTATAATGACGAGGGGGAAGCAGAGCACGAGCATGGGTTTACAGATAGAAGGGGAATCATCATAGCAATTGTTTTGGGAGCAGTTGCTTTCACAATGCTGTCTCTCTTTGCTGCTTATGCAGGCTATACAAAGCTGGTGCAGATCAGAAAAGAGTGCAGTAATCTAGGCTTGGTGTCAATAAGCATTAGCAAGTATTCTGGCTTGAGTTTCAAGTATGAAACACTAGAGAAGGCCACAGATTATTTTAACACCTCGAGAAAAATAGGGGAAGGGGGAGGTGGTACTGTGTTTAAGGGGACTCTTCCGAATCGCAAAACCGTAGCTGTTAAGAGATTGTTCTACAATACAAGGCAATGGGTAGATGAGTTCTTCAATGAGGTGAATTTGATCAGTGGAATTCAGCACAAGCACCTAGTGAAGCTTTTGGGTTGTAGCATTGAAGGCCCTGAGAGCCTGCTGGTGTATGAATATGTGCCTAACAGGAGCCTCGATCAATTCCTTTTCGATAAGAACAATGATCAGTTTCTTAACTGGAAGCAGCGGTTTGATATTATTGTCGGAATAGCTGAAGGGATTGCGTATCTTCATGAAGGCTCTGAAGTAAGAATAATCCACAGGGACATAAAAAGCAGCAATGTTCTTCTGGATGAGAATCTTAGCCCAAAGATTGCAGACTTTGGCCTTGCTCGGTGTTTTGCTTCCGACAAGTCTCATCTTAGCACCGGCATTGCTGGAACACTAGGTTATATGGCCCCAGAGTATCTTGTTCAAGGGCAACTTACAGAGAAAGCAGACGTTTATAGCTTCGGAGTGCTAGTTCTCGAGATTGTGTCTGGTATGAGGAATAGCACCTTCAAACAGGCGGACACTGACTTTGATTCACTACTACACAAAGTTTGGAAACACTACAGATCAAACGAGTTGGCCGACGTGGTTGATCCTTGCTTGAGAGATCATGAGTTGCCAGCAAAAGAGGTATCAAGTGTTCTTCAAATTGGACTTCTGTGCACACAGGCCTCAGTTGCTTTGAGACCATCTATGGCTGAAGTAGTTCTAATGCTAACAGCCAACAAAGAAAGTGACATTCCCATTCCAAACCAACCTCCCTTTCTAAATGCCACGACGCTAGAGCAAGCCAGCTCCATTAGGTCTAATAGCGCAAACAGCTTCGTCTCAAATACTTTAACGAAAGTTCAGACGTCCTACACCTCATCAGAGTCTTCCACCATGCCTAGTTTGGATAATATGGGACATATATCAACAAAATGA

Protein Analysis

1053

Amino Acids

117.71

Weight (kDa)

5.91

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 4 - 67 3.1e-09 Salt stress response/antifungal
Stress-antifung PF01657 82 - 177 1.5e-11 Salt stress response/antifungal
Pkinase PF00069 222 - 307 1.9e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 225 - 308 2.8e-13 Protein tyrosine and serine/threonine kinase
Stress-antifung PF01657 438 - 523 2.7e-10 Salt stress response/antifungal
Stress-antifung PF01657 539 - 628 2.4e-11 Salt stress response/antifungal
Pkinase PF00069 713 - 936 7.1e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 715 - 983 9.3e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AasI GACNNNNNNGTC 1 cut(s) 1610
AatII GACGTC 1 cut(s) 3095
AccB1I GGYRCC 1 cut(s) 1391
AccI GTMKAC 1 cut(s) 1602
AccII CGCG 2 cut(s) 1640, 1694
AclI AACGTT 2 cut(s) 748, 1659
AclWI GGATC 3 cut(s) 1259, 1492, 2825
AcoI YGGCCR 2 cut(s) 1618, 2817
AcsI RAATTY 6 cut(s) 272, 622, 795, 884, 2275, 2289
AcuI CTGAAG 4 cut(s) 1572, 2481, 2511, 2958
AcyI GRCGYC 1 cut(s) 3092
AdeI CACNNNGTG 1 cut(s) 2308
AfaI GTAC 5 cut(s) 632, 788, 946, 1424, 2176
AfiI CCNNNNNNNGG 4 cut(s) 152, 1209, 1424, 1627
AhlI ACTAGT 2 cut(s) 640, 941
AjiI CACGTC 2 cut(s) 105, 2824
AjuI GAANNNNNNNTTGG 2 cut(s) 2985, 3017
AleI CACNNNNGTG 1 cut(s) 952
AloI GAACNNNNNNTCC 2 cut(s) 2694, 2726
Alw21I GWGCWC 5 cut(s) 498, 1061, 1371, 1920, 2903
Alw26I GTCTC 6 cut(s) 209, 1116, 1998, 2607, 2917, 3070
Alw44I GTGCAC 2 cut(s) 1057, 2899
AlwI GGATC 3 cut(s) 1259, 1492, 2825
Ama87I CYCGRG 3 cut(s) 549, 2146, 2714
AoxI GGCC 9 cut(s) 1285, 1426, 1618, 2124, 2335, 2572, 2643, 2817, 2907
ApaLI GTGCAC 2 cut(s) 1057, 2899
ApoI RAATTY 6 cut(s) 272, 622, 795, 884, 2275, 2289
ArsI GACNNNNNNTTYG 6 cut(s) 67, 99, 1575, 1607, 2750, 2782
Asp700I GAANNNNTTC 2 cut(s) 296, 2943
AspLEI GCGC 1 cut(s) 3053
AspS9I GGNCC 4 cut(s) 958, 1689, 2336, 2644
AsuII TTCGAA 1 cut(s) 1105
AvaI CYCGRG 3 cut(s) 549, 2146, 2714
AvaII GGWCC 2 cut(s) 958, 1689
BaeGI GKGCMC 4 cut(s) 716, 1061, 1394, 2903
BanI GGYRCC 1 cut(s) 1391
BanII GRGCYC 2 cut(s) 556, 1371
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BauI CACGAG 1 cut(s) 1919
BbsI GAAGAC 5 cut(s) 1020, 1460, 1769, 1841, 3105
Bbv12I GWGCWC 5 cut(s) 498, 1061, 1371, 1920, 2903
BccI CCATC 9 cut(s) 167, 326, 484, 583, 719, 1150, 1766, 1772, 2935
BceAI ACGGC 2 cut(s) 1441, 1558
BcgI CGANNNNNNTGC 2 cut(s) 1519, 1553
BclI TGATCA 2 cut(s) 2280, 2410
BcoDI GTCTC 6 cut(s) 209, 1116, 1998, 2607, 2917, 3070
BcuI ACTAGT 2 cut(s) 640, 941
BfmI CTRYAG 2 cut(s) 1567, 2800
BglII AGATCT 1 cut(s) 79
BlpI GCTNAGC 1 cut(s) 321
BmcAI AGTACT 1 cut(s) 632
Bme18I GGWCC 2 cut(s) 958, 1689
BmeT110I CYCGRG 3 cut(s) 549, 2146, 2714
BmgBI CACGTC 2 cut(s) 105, 2824
BmgT120I GGNCC 4 cut(s) 958, 1689, 2336, 2644
BmiI GGNNCC 4 cut(s) 959, 1393, 2378, 2646
BmrI ACTGGG 1 cut(s) 1396
BmsI GCATC 1 cut(s) 1539
BmuI ACTGGG 1 cut(s) 1396
BpiI GAAGAC 5 cut(s) 1020, 1460, 1769, 1841, 3105
BpmI CTGGAG 1 cut(s) 424
Bpu10I CCTNAGC 1 cut(s) 570
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 1105
BpuEI CTTGAG 5 cut(s) 762, 1047, 1891, 2116, 2860
Bsa29I ATCGAT 1 cut(s) 993
BsaBI GATNNNNATC 1 cut(s) 2544
BsaHI GRCGYC 1 cut(s) 3092
BsaI GGTCTC 1 cut(s) 2917
BsaJI CCNNGG 5 cut(s) 129, 841, 1183, 1275, 1692
BsaWI WCCGGW 1 cut(s) 761
BsaXI ACNNNNNCTCC 2 cut(s) 2694, 2724
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 1209, 1424, 1627
Bse118I RCCGGY 2 cut(s) 48, 2615
Bse1I ACTGG 2 cut(s) 1391, 2429
Bse3DI GCAATG 5 cut(s) 241, 1130, 2252, 2533, 2619
Bse8I GATNNNNATC 1 cut(s) 2544
BseCI ATCGAT 1 cut(s) 993
BseDI CCNNGG 5 cut(s) 129, 841, 1183, 1275, 1692
BseGI GGATG 3 cut(s) 1777, 1868, 2545
BseJI GATNNNNATC 1 cut(s) 2544
BseLI CCNNNNNNNGG 4 cut(s) 152, 1209, 1424, 1627
BseMI GCAATG 5 cut(s) 241, 1130, 2252, 2533, 2619
BseMII CTCAG 6 cut(s) 80, 312, 474, 624, 2331, 2925
BseNI ACTGG 2 cut(s) 1391, 2429
BseRI GAGGAG 1 cut(s) 421
BseSI GKGCMC 4 cut(s) 716, 1061, 1394, 2903
BsgI GTGCAG 2 cut(s) 2051, 2068
Bsh1236I CGCG 2 cut(s) 1640, 1694
BshFI GGCC 9 cut(s) 1287, 1428, 1620, 2126, 2337, 2574, 2645, 2819, 2909
BshNI GGYRCC 1 cut(s) 1391
BshVI ATCGAT 1 cut(s) 993
BsiHKAI GWGCWC 5 cut(s) 498, 1061, 1371, 1920, 2903
BsiHKCI CYCGRG 3 cut(s) 549, 2146, 2714
BsiSI CCGG 3 cut(s) 49, 762, 2616
BslFI GGGAC 6 cut(s) 34, 971, 1577, 2203, 2525, 3156
BslI CCNNNNNNNGG 4 cut(s) 152, 1209, 1424, 1627
BsmAI GTCTC 6 cut(s) 209, 1116, 1998, 2607, 2917, 3070
BsmBI CGTCTC 1 cut(s) 3070
BsmFI GGGAC 6 cut(s) 34, 971, 1577, 2203, 2525, 3156
BsmI GAATGC 3 cut(s) 1113, 1442, 1861
BsnI GGCC 9 cut(s) 1287, 1428, 1620, 2126, 2337, 2574, 2645, 2819, 2909
Bso31I GGTCTC 1 cut(s) 2917
BsoBI CYCGRG 3 cut(s) 549, 2146, 2714
Bsp119I TTCGAA 1 cut(s) 1105
Bsp1286I GDGCHC 8 cut(s) 498, 556, 716, 1061, 1371, 1394, 1920, 2903
Bsp1407I TGTACA 1 cut(s) 786
Bsp1720I GCTNAGC 1 cut(s) 321
BspANI GGCC 9 cut(s) 1287, 1428, 1620, 2126, 2337, 2574, 2645, 2819, 2909
BspCNI CTCAG 6 cut(s) 79, 313, 475, 623, 2332, 2924
BspDI ATCGAT 1 cut(s) 993
BspFNI CGCG 2 cut(s) 1640, 1694
BspHI TCATGA 2 cut(s) 2479, 2848
BspLI GGNNCC 4 cut(s) 959, 1393, 2378, 2646
BspPI GGATC 3 cut(s) 1259, 1492, 2825
BspQI GCTCTTC 1 cut(s) 1220
BspT104I TTCGAA 1 cut(s) 1105
BspT107I GGYRCC 1 cut(s) 1391
BspTNI GGTCTC 1 cut(s) 2917
BsrDI GCAATG 5 cut(s) 241, 1130, 2252, 2533, 2619
BsrFI RCCGGY 2 cut(s) 48, 2615
BsrGI TGTACA 1 cut(s) 786
BsrI ACTGG 2 cut(s) 1391, 2429
BssAI RCCGGY 2 cut(s) 48, 2615
BssECI CCNNGG 5 cut(s) 129, 841, 1183, 1275, 1692
BssNI GRCGYC 1 cut(s) 3092
BssSI CACGAG 1 cut(s) 1919
BssT1I CCWWGG 3 cut(s) 129, 841, 1183
Bst2BI CACGAG 1 cut(s) 1919
Bst4CI ACNGT 6 cut(s) 382, 419, 458, 1768, 2179, 2212
Bst6I CTCTTC 5 cut(s) 24, 1114, 1220, 1620, 2199
BstACI GRCGYC 1 cut(s) 3092
BstAUI TGTACA 1 cut(s) 786
BstBI TTCGAA 1 cut(s) 1105
BstDEI CTNAG 9 cut(s) 66, 321, 483, 570, 610, 2340, 2549, 2609, 2911
BstDSI CCRYGG 1 cut(s) 1692
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 3 cut(s) 1777, 1868, 2545
BstFNI CGCG 2 cut(s) 1640, 1694
BstHHI GCGC 1 cut(s) 3053
BstMAI GTCTC 6 cut(s) 209, 1116, 1998, 2607, 2917, 3070
BstNSI RCATGY 1 cut(s) 1192
BstSFI CTRYAG 2 cut(s) 1567, 2800
BstSLI GKGCMC 4 cut(s) 716, 1061, 1394, 2903
BstUI CGCG 2 cut(s) 1640, 1694
BstV2I GAAGAC 5 cut(s) 1020, 1460, 1769, 1841, 3105
BstX2I RGATCY 2 cut(s) 79, 1497
BstXI CCANNNNNNTGG 1 cut(s) 680
BstYI RGATCY 2 cut(s) 79, 1497
Bsu15I ATCGAT 1 cut(s) 993
BsuRI GGCC 9 cut(s) 1287, 1428, 1620, 2126, 2337, 2574, 2645, 2819, 2909
BsuTUI ATCGAT 1 cut(s) 993
BtgI CCRYGG 1 cut(s) 1692
BtrI CACGTC 2 cut(s) 105, 2824
BtsCI GGATG 3 cut(s) 1777, 1868, 2545
BtsI GCAGTG 2 cut(s) 1650, 1760
BtsIMutI CAGTG 8 cut(s) 74, 387, 1384, 1458, 1650, 1760, 2290, 2760
CciI TCATGA 2 cut(s) 2479, 2848
CfoI GCGC 1 cut(s) 3053
Cfr10I RCCGGY 2 cut(s) 48, 2615
Cfr13I GGNCC 4 cut(s) 958, 1689, 2336, 2644
Cfr42I CCGCGG 1 cut(s) 1695
ClaI ATCGAT 1 cut(s) 993
CseI GACGC 2 cut(s) 1646, 3025
Csp6I GTAC 5 cut(s) 631, 787, 945, 1423, 2175
CviQI GTAC 5 cut(s) 631, 787, 945, 1423, 2175
DdeI CTNAG 9 cut(s) 66, 321, 483, 570, 610, 2340, 2549, 2609, 2911
DraIII CACNNNGTG 1 cut(s) 2308
DrdI GACNNNNNNGTC 1 cut(s) 1610
DseDI GACNNNNNNGTC 1 cut(s) 1610
EaeI YGGCCR 2 cut(s) 1618, 2817
Eam1104I CTCTTC 5 cut(s) 24, 1114, 1220, 1620, 2199
EarI CTCTTC 5 cut(s) 24, 1114, 1220, 1620, 2199
EciI GGCGGA 4 cut(s) 1513, 1649, 1712, 2771
Ecl136II GAGCTC 1 cut(s) 1369
Eco130I CCWWGG 3 cut(s) 129, 841, 1183
Eco147I AGGCCT 1 cut(s) 2909
Eco24I GRGCYC 2 cut(s) 556, 1371
Eco31I GGTCTC 1 cut(s) 2917
Eco47I GGWCC 2 cut(s) 958, 1689
Eco53kI GAGCTC 1 cut(s) 1369
Eco57I CTGAAG 4 cut(s) 1572, 2481, 2511, 2958
Eco88I CYCGRG 3 cut(s) 549, 2146, 2714
EcoICRI GAGCTC 1 cut(s) 1369
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO109I RGGNCCY 1 cut(s) 2336
EcoRI GAATTC 2 cut(s) 884, 2289
EcoT14I CCWWGG 3 cut(s) 129, 841, 1183
EcoT22I ATGCAT 1 cut(s) 1194
EcoT38I GRGCYC 2 cut(s) 556, 1371
ErhI CCWWGG 3 cut(s) 129, 841, 1183
Esp3I CGTCTC 1 cut(s) 3070
FalI AAGNNNNNCTT 2 cut(s) 2655, 2687
FaqI GGGAC 6 cut(s) 34, 971, 1577, 2203, 2525, 3156
FauI CCCGC 3 cut(s) 1511, 1535, 1603
FauNDI CATATG 1 cut(s) 694
FbaI TGATCA 2 cut(s) 2280, 2410
FblI GTMKAC 1 cut(s) 1602
FokI GGATG 3 cut(s) 1784, 1875, 2552
FriOI GRGCYC 2 cut(s) 556, 1371
GlaI GCGC 1 cut(s) 3052
GsuI CTGGAG 1 cut(s) 424
HaeIII GGCC 9 cut(s) 1287, 1428, 1620, 2126, 2337, 2574, 2645, 2819, 2909
HapII CCGG 3 cut(s) 49, 762, 2616
HgaI GACGC 2 cut(s) 1646, 3025
HhaI GCGC 1 cut(s) 3053
Hin1I GRCGYC 1 cut(s) 3092
Hin6I GCGC 1 cut(s) 3051
HinP1I GCGC 1 cut(s) 3051
HincII GTYRAC 1 cut(s) 1603
HindII GTYRAC 1 cut(s) 1603
HindIII AAGCTT 3 cut(s) 677, 1584, 2312
HinfI GANTC 9 cut(s) 409, 1604, 1882, 1947, 2191, 2200, 2545, 2771, 3110
HpaII CCGG 3 cut(s) 49, 762, 2616
Hpy166II GTNNAC 6 cut(s) 787, 947, 1059, 1603, 1932, 2901
Hpy8I GTNNAC 6 cut(s) 787, 947, 1059, 1603, 1932, 2901
Hpy99I CGWCG 4 cut(s) 1544, 1613, 2825, 3019
HpyCH4III ACNGT 6 cut(s) 382, 419, 458, 1768, 2179, 2212
HpyCH4IV ACGT 8 cut(s) 104, 517, 748, 1081, 1659, 2688, 2823, 3092
HpyF3I CTNAG 9 cut(s) 66, 321, 483, 570, 610, 2340, 2549, 2609, 2911
HpySE526I ACGT 8 cut(s) 104, 517, 748, 1081, 1659, 2688, 2823, 3092
Hsp92I GRCGYC 1 cut(s) 3092
HspAI GCGC 1 cut(s) 3051
Ksp22I TGATCA 2 cut(s) 2280, 2410
KspI CCGCGG 1 cut(s) 1695
LguI GCTCTTC 1 cut(s) 1220
LmnI GCTCC 6 cut(s) 9, 689, 1298, 1970, 2376, 3038
LweI GCATC 1 cut(s) 1539
MaeII ACGT 8 cut(s) 104, 517, 748, 1081, 1659, 2688, 2823, 3092
MaeIII GTNAC 5 cut(s) 8, 518, 1077, 1865, 2972
MfeI CAATTG 1 cut(s) 1959
MflI RGATCY 2 cut(s) 79, 1497
MhlI GDGCHC 8 cut(s) 498, 556, 716, 1061, 1371, 1394, 1920, 2903
MlyI GAGTC 5 cut(s) 418, 1598, 1876, 2185, 3119
MmeI TCCRAC 3 cut(s) 1358, 2431, 2619
Mph1103I ATGCAT 1 cut(s) 1194
MroXI GAANNNNTTC 2 cut(s) 296, 2943
MslI CAYNNNNRTG 3 cut(s) 330, 952, 1923
MspA1I CMGCKG 4 cut(s) 867, 1506, 1694, 2434
MspI CCGG 3 cut(s) 49, 762, 2616
MunI CAATTG 1 cut(s) 1959
Mva1269I GAATGC 3 cut(s) 1113, 1442, 1861
MvnI CGCG 2 cut(s) 1640, 1694
NdeI CATATG 1 cut(s) 694
NlaIV GGNNCC 4 cut(s) 959, 1393, 2378, 2646
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 2972
NsiI ATGCAT 1 cut(s) 1194
NspI RCATGY 1 cut(s) 1192
NspV TTCGAA 1 cut(s) 1105
OliI CACNNNNGTG 1 cut(s) 952
PaeI GCATGC 1 cut(s) 1192
PaeR7I CTCGAG 3 cut(s) 549, 2146, 2714
PagI TCATGA 2 cut(s) 2479, 2848
PceI AGGCCT 1 cut(s) 2909
PciSI GCTCTTC 1 cut(s) 1220
PcsI WCGNNNNNNNCGW 2 cut(s) 1608, 2817
PctI GAATGC 3 cut(s) 1113, 1442, 1861
PdmI GAANNNNTTC 2 cut(s) 296, 2943
PfeI GAWTC 4 cut(s) 1947, 2200, 2545, 2771
PflFI GACNNNGTC 2 cut(s) 962, 1607
PleI GAGTC 5 cut(s) 417, 1598, 1876, 2185, 3118
PpsI GAGTC 5 cut(s) 417, 1598, 1876, 2185, 3118
PsiI TTATAA 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 1371
Psp1406I AACGTT 2 cut(s) 748, 1659
PspN4I GGNNCC 4 cut(s) 959, 1393, 2378, 2646
PspPI GGNCC 4 cut(s) 958, 1689, 2336, 2644
PspXI VCTCGAGB 1 cut(s) 549
PsrI GAACNNNNNNTAC 4 cut(s) 412, 444, 2862, 2894
PsuI RGATCY 2 cut(s) 79, 1497
PsyI GACNNNGTC 2 cut(s) 962, 1607
PvuII CAGCTG 1 cut(s) 867
RsaI GTAC 5 cut(s) 632, 788, 946, 1424, 2176
RsaNI GTAC 5 cut(s) 631, 787, 945, 1423, 2175
RseI CAYNNNNRTG 3 cut(s) 330, 952, 1923
SacI GAGCTC 1 cut(s) 1371
SacII CCGCGG 1 cut(s) 1695
SalI GTCGAC 1 cut(s) 1601
SapI GCTCTTC 1 cut(s) 1220
Sau96I GGNCC 4 cut(s) 958, 1689, 2336, 2644
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 5 cut(s) 418, 1598, 1876, 2185, 3119
SduI GDGCHC 8 cut(s) 498, 556, 716, 1061, 1371, 1394, 1920, 2903
SfaNI GCATC 1 cut(s) 1539
SfcI CTRYAG 2 cut(s) 1567, 2800
Sfr274I CTCGAG 3 cut(s) 549, 2146, 2714
Sfr303I CCGCGG 1 cut(s) 1695
SfuI TTCGAA 1 cut(s) 1105
SgrBI CCGCGG 1 cut(s) 1695
SinI GGWCC 2 cut(s) 958, 1689
SlaI CTCGAG 3 cut(s) 549, 2146, 2714
SmiMI CAYNNNNRTG 3 cut(s) 330, 952, 1923
SmlI CTYRAG 8 cut(s) 549, 777, 1062, 1870, 2095, 2146, 2714, 2839
SmoI CTYRAG 8 cut(s) 549, 777, 1062, 1870, 2095, 2146, 2714, 2839
SpeI ACTAGT 2 cut(s) 640, 941
SphI GCATGC 1 cut(s) 1192
SseBI AGGCCT 1 cut(s) 2909
SstI GAGCTC 1 cut(s) 1371
StuI AGGCCT 1 cut(s) 2909
StyI CCWWGG 3 cut(s) 129, 841, 1183
TaaI ACNGT 6 cut(s) 382, 419, 458, 1768, 2179, 2212
TaiI ACGT 8 cut(s) 107, 520, 751, 1084, 1662, 2691, 2826, 3095
TaqII GACCGA 1 cut(s) 76
TatI WGTACW 3 cut(s) 630, 786, 944
TauI GCSGC 6 cut(s) 1484, 1491, 1620, 1634, 1643, 1697
TfiI GAWTC 4 cut(s) 1947, 2200, 2545, 2771
TscAI CASTG 8 cut(s) 74, 387, 1391, 1465, 1650, 1760, 2290, 2767
TseFI GTSAC 1 cut(s) 2972
Tsp45I GTSAC 1 cut(s) 2972
TspGWI ACGGA 1 cut(s) 1597
TspRI CASTG 8 cut(s) 74, 387, 1391, 1465, 1650, 1760, 2290, 2767
Tth111I GACNNNGTC 2 cut(s) 962, 1607
VneI GTGCAC 2 cut(s) 1057, 2899
VpaK11BI GGWCC 2 cut(s) 958, 1689
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 6 cut(s) 272, 622, 795, 884, 2275, 2289
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 1 cut(s) 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 3127
XhoI CTCGAG 3 cut(s) 549, 2146, 2714
XmiI GTMKAC 1 cut(s) 1602
XmnI GAANNNNTTC 2 cut(s) 296, 2943
ZraI GACGTC 1 cut(s) 3093
ZrmI AGTACT 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.