Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001832

Physical Location & Seq

Genomic Coordinates Forward (+)

80586158 .. 80589684

Gene Structure 3527 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G052670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGCATACCAAAACCTCAACATGGCTCTTCTTCTTCATCTTCATCTTCTTCTCGCTTTTCTACCTTTTTCTCTGTGATCCCAGAACCTCGGAAGCTGGCCTCTACTGCGGAGCTGCCAAGCCACCACCAAACGCTAGCTTCATTCCAACTTTTGTCGCAGAAATGGAGAGTTTATCAGAGCTCATCACCAAACACCACTGGGGCACCCATTTCTTTAACTCCACGACCCCCATGTACGGCCTAGCGGAATGCTACAACGACCTGTCGCACACTGATTGTCTTCTCTGCTACGCCGCCAGCCGCACCGAGATCCCCCGCTGCCTCCCCTCCCTCTCCGCCCGCATCTTTCTCGACGGCTGCTTCCTCCGCTACGACAACTACAGCTTCTACTCCGAAGCTTCAGTCCCCGCAGTCGACTCCGTCGTCTGCGGCCAAGAAGAGGCGGCGGACGCGGCCGTGTTTAAGGAGAACGTTGCGGAGGTGATTGAGAGGGTTAAGAGGTCCGCGGCGGAGAATGGGGGGTTTGGGGTAGGGGAAGTGGGTGGGGAGGTGTATGCTTTGGGGCAGTGCTGGAAGACGGTGGATGGTGATGGGTGTAGAGAGTGTTTGGAGAAGGGAGGTGAGGCGGTGAGAGGCTGTGGGTTGAAGACAGAAGGCAAGGCTTTGAATGCTGGATGTTACTTGAGGTATTCGACTCGGAAGTTCTATAATGACGAGGGGGAAGCAGAGCACGAGCATGGGTTTACAGATAGAAGGGGAATCATCATAGCAATTGTTTTGGGAGCAGTTGCTTTCACAATGCTGTCTCTCTTTGCTGCTTATGCAGGCTATACAAAGCTGGTGCAGATCAGAAAAGAGTGCAGTAATCTAGGCTTGGTGTCAATAAGCATTAGCAAGTATTCTGGCTTGAGTTTCAAGTATGAAACACTAGAGAAGGCCACAGATTATTTTAACACCTCGAGAAAAATAGGGGAAGGGGGAGGTGGTACTGTGTTTAAGGGGACTCTTCCGAATCGCAAAACCGTAGCTGTTAAGAGATTGTTCTACAATACAAGGCAATGGGTAGATGAGTTCTTCAATGAGGTGAATTTGATCAGTGGAATTCAGCACAAGCACCTAGTGAAGCTTTTGGGTTGCAGCATTGAAGGCCCTGAGAGCCTGCTGGTGTATGAATATGTGCCTAACAGGAGCCTCGATCAATTCCTTTTCGATAAGAACAATGATCAGTTTCTTAACTGGAAGCAGCGGTTTGATATTATTGTCGGAATAGCTGAAGGGATTGCGTATCTTCATGAAGGCTCTGAAGTAAGAATAATCCACAGGGACATAAAAAGCAGCAATGTTCTTCTGGATGAGAATCTTAGCCCAAAGATTGCAGACTTTGGCCTTGCTCGGTGTTTTGCTTCCGACAAGTCTCATCTTAGCACCGGCATTGCTGGAACACTAGGTTATATGGCCCCAGAGTATCTTGTTCAAGGGCAACTTACAGAGAAAGCAGACGTTTATAGCTTCGGAGTGCTAGTTCTCGAGATTGTGTCTGGTATGAGGAATAGCACCTTCAAACAGGCGGACACTGACTTTGATTCACTACTACACAAAGTTTGGAAACACTACAGATCAAACGAGTTGGCCGACGTGGTTGATCCTTGCTTGAGAGATCATGAGTTGCCAGCAAAAGAGGTATCAAATGTTCTTCAAATTGGACTTCTTTGCACACAGGCCTCAGTTGCTTTGAGACCATCTATGGCTGAAGTAGTTCTAATGCTAACAGCCAACAAAGAAAGTGACATTCCCATTCCAAACCAACCTCCCTTTCTAAATGCCACGACGCTAGAGCAAGCCAGCTCCATTAGGTCTAATAGCGCAAACAGCTTCGTCTCAAATACTTTAACGAAAGTTCAGACGTCCTACACCTCATCAGAGTCTTCCACCATGCCTAGTTTGGATAATATGGGACATATATCAACAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

657

Amino Acids

72.79

Weight (kDa)

5.67

Isoelectric Point (pI)

34.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	50 - 127	2.8e-10	Salt stress response/antifungal
Stress-antifung	PF01657	143 - 232	1.3e-11	Salt stress response/antifungal
Pkinase	PF00069	317 - 540	2.7e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	319 - 587	3.1e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40380 AT5G40380 AT5G40380
fragaria_vesca	FvH4_4g26070 FvH4_5g05520 FvH4_6g51060 FvH4_6g51060 FvH4_6g51060 FvH4_6g51060 FvH4_6g51060
malus_domestica	MD14G1177300.v1.1 MD17G1031100.v1.1
prunus_persica	Prupe.3G288600_v2.0.a1 Prupe.5G171200_v2.0.a1
pyrus_communis	pycom14g14810 pycom17g02850
rosa_chinensis	RchiOBHm_Chr2g0089301 RchiOBHm_Chr2g0172611 RchiOBHm_Chr7g0193291
rosa_laevigata	RLG00000004281 RLG00000022120 RLG00000022121 RLG00000022125
rosa_multiflora	Rmu_co8025498.1_g000001 Rmu_sc0017093.1_g000001
rosa_roxburghii	Rroxscaffold_2G00079690 Rroxscaffold_2G00079700 Rroxscaffold_3G00262230
rosa_rugosa	Rorug01G0488900 Rorug02G0246100 Rorug02G0560300 Rorug02G0560400 Rorug07G0008700
rosa_samantha	Rh2AG636700 Rh2AG636900 Rh2BG650100 Rh2BG650300 Rh2CG616800 Rh2CG616900 Rh2DG664300 Rh2DG664400 Rh7BG135300 Rh7CG138300 Rh7DG136300
rosa_wichuraiana	Rw2G052660 Rw2G052670 Rw7G011490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	422
AatII	GACGTC	1 cut(s)	1907
AccB1I	GGYRCC	1 cut(s)	203
AccI	GTMKAC	1 cut(s)	414
AccII	CGCG	2 cut(s)	452, 506
AclI	AACGTT	1 cut(s)	471
AclWI	GGATC	3 cut(s)	71, 304, 1637
AcoI	YGGCCR	3 cut(s)	430, 453, 1629
AcsI	RAATTY	2 cut(s)	1087, 1101
AcuI	CTGAAG	4 cut(s)	384, 1293, 1323, 1770
AcyI	GRCGYC	1 cut(s)	1904
AdeI	CACNNNGTG	1 cut(s)	1120
AfaI	GTAC	2 cut(s)	236, 988
AfiI	CCNNNNNNNGG	3 cut(s)	21, 236, 439
AgsI	TTSAA	8 cut(s)	646, 667, 916, 1078, 1145, 1475, 1561, 1697
AjiI	CACGTC	1 cut(s)	1636
AjuI	GAANNNNNNNTTGG	2 cut(s)	1797, 1829
AloI	GAACNNNNNNTCC	2 cut(s)	1506, 1538
Alw21I	GWGCWC	2 cut(s)	183, 732
Alw26I	GTCTC	4 cut(s)	810, 1419, 1729, 1882
AlwI	GGATC	3 cut(s)	71, 304, 1637
Ama87I	CYCGRG	2 cut(s)	958, 1526
ApeKI	GCWGC	7 cut(s)	113, 318, 357, 815, 1137, 1243, 1335
ApoI	RAATTY	2 cut(s)	1087, 1101
ArsI	GACNNNNNNTTYG	4 cut(s)	387, 419, 1562, 1594
Asp700I	GAANNNNTTC	1 cut(s)	1755
AspLEI	GCGC	1 cut(s)	1865
AspS9I	GGNCC	3 cut(s)	501, 1148, 1456
AsuHPI	GGTGA	6 cut(s)	178, 493, 599, 632, 640, 1096
AsuNHI	GCTAGC	1 cut(s)	134
AvaI	CYCGRG	2 cut(s)	958, 1526
AvaII	GGWCC	1 cut(s)	501
BaeGI	GKGCMC	1 cut(s)	206
BanI	GGYRCC	1 cut(s)	203
BanII	GRGCYC	1 cut(s)	183
BauI	CACGAG	1 cut(s)	731
BbsI	GAAGAC	4 cut(s)	272, 581, 653, 1917
Bbv12I	GWGCWC	2 cut(s)	183, 732
BbvI	GCAGC	7 cut(s)	100, 305, 344, 802, 1149, 1255, 1347
BccI	CCATC	3 cut(s)	578, 584, 1747
BceAI	ACGGC	3 cut(s)	253, 370, 440
BcgI	CGANNNNNNTGC	2 cut(s)	331, 365
BclI	TGATCA	2 cut(s)	1092, 1222
BcoDI	GTCTC	4 cut(s)	810, 1419, 1729, 1882
BfaI	CTAG	9 cut(s)	135, 242, 869, 929, 1118, 1445, 1520, 1832, 1938
BfmI	CTRYAG	2 cut(s)	379, 1612
Bme18I	GGWCC	1 cut(s)	501
BmeT110I	CYCGRG	2 cut(s)	958, 1526
BmgBI	CACGTC	1 cut(s)	1636
BmgT120I	GGNCC	3 cut(s)	501, 1148, 1456
BmiI	GGNNCC	3 cut(s)	205, 1190, 1458
BmrI	ACTGGG	1 cut(s)	208
BmsI	GCATC	1 cut(s)	351
BmtI	GCTAGC	1 cut(s)	138
BmuI	ACTGGG	1 cut(s)	208
BpiI	GAAGAC	4 cut(s)	272, 581, 653, 1917
BpuEI	CTTGAG	3 cut(s)	703, 928, 1672
BsaBI	GATNNNNATC	1 cut(s)	1356
BsaHI	GRCGYC	1 cut(s)	1904
BsaI	GGTCTC	1 cut(s)	1729
BsaJI	CCNNGG	2 cut(s)	87, 504
BsaXI	ACNNNNNCTCC	2 cut(s)	1506, 1536
Bsc4I	CCNNNNNNNGG	3 cut(s)	21, 236, 439
Bse118I	RCCGGY	1 cut(s)	1427
Bse1I	ACTGG	2 cut(s)	203, 1241
Bse3DI	GCAATG	3 cut(s)	1064, 1345, 1431
Bse8I	GATNNNNATC	1 cut(s)	1356
BseDI	CCNNGG	2 cut(s)	87, 504
BseGI	GGATG	3 cut(s)	589, 680, 1357
BseJI	GATNNNNATC	1 cut(s)	1356
BseLI	CCNNNNNNNGG	3 cut(s)	21, 236, 439
BseMI	GCAATG	3 cut(s)	1064, 1345, 1431
BseMII	CTCAG	2 cut(s)	1143, 1737
BseNI	ACTGG	2 cut(s)	203, 1241
BseSI	GKGCMC	1 cut(s)	206
BseX3I	CGGCCG	1 cut(s)	453
BseXI	GCAGC	7 cut(s)	100, 305, 344, 802, 1149, 1255, 1347
BsgI	GTGCAG	2 cut(s)	863, 880
Bsh1236I	CGCG	2 cut(s)	452, 506
Bsh1285I	CGRYCG	1 cut(s)	456
BshNI	GGYRCC	1 cut(s)	203
BsiEI	CGRYCG	1 cut(s)	456
BsiHKAI	GWGCWC	2 cut(s)	183, 732
BsiHKCI	CYCGRG	2 cut(s)	958, 1526
BsiSI	CCGG	1 cut(s)	1428
BslFI	GGGAC	4 cut(s)	389, 1015, 1337, 1968
BslI	CCNNNNNNNGG	3 cut(s)	21, 236, 439
BsmAI	GTCTC	4 cut(s)	810, 1419, 1729, 1882
BsmBI	CGTCTC	1 cut(s)	1882
BsmFI	GGGAC	4 cut(s)	389, 1015, 1337, 1968
BsmI	GAATGC	2 cut(s)	254, 673
Bso31I	GGTCTC	1 cut(s)	1729
BsoBI	CYCGRG	2 cut(s)	958, 1526
Bsp1286I	GDGCHC	3 cut(s)	183, 206, 732
Bsp143I	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
BspCNI	CTCAG	2 cut(s)	1144, 1736
BspFNI	CGCG	2 cut(s)	452, 506
BspHI	TCATGA	2 cut(s)	1291, 1660
BspLI	GGNNCC	3 cut(s)	205, 1190, 1458
BspOI	GCTAGC	1 cut(s)	138
BspPI	GGATC	3 cut(s)	71, 304, 1637
BspQI	GCTCTTC	1 cut(s)	32
BspT107I	GGYRCC	1 cut(s)	203
BspTNI	GGTCTC	1 cut(s)	1729
BsrDI	GCAATG	3 cut(s)	1064, 1345, 1431
BsrFI	RCCGGY	1 cut(s)	1427
BsrI	ACTGG	2 cut(s)	203, 1241
BssAI	RCCGGY	1 cut(s)	1427
BssECI	CCNNGG	2 cut(s)	87, 504
BssMI	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
BssNI	GRCGYC	1 cut(s)	1904
BssSI	CACGAG	1 cut(s)	731
Bst2BI	CACGAG	1 cut(s)	731
Bst4CI	ACNGT	3 cut(s)	580, 991, 1024
Bst6I	CTCTTC	3 cut(s)	32, 432, 1011
BstACI	GRCGYC	1 cut(s)	1904
BstC8I	GCNNGC	9 cut(s)	97, 136, 298, 340, 826, 1160, 1671, 1839, 1843
BstDEI	CTNAG	4 cut(s)	1152, 1361, 1421, 1723
BstDSI	CCRYGG	1 cut(s)	504
BstF5I	GGATG	3 cut(s)	589, 680, 1357
BstFNI	CGCG	2 cut(s)	452, 506
BstHHI	GCGC	1 cut(s)	1865
BstKTI	GATC	9 cut(s)	79, 312, 849, 1095, 1198, 1225, 1619, 1645, 1660
BstMAI	GTCTC	4 cut(s)	810, 1419, 1729, 1882
BstMBI	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
BstMCI	CGRYCG	1 cut(s)	456
BstSFI	CTRYAG	2 cut(s)	379, 1612
BstSLI	GKGCMC	1 cut(s)	206
BstUI	CGCG	2 cut(s)	452, 506
BstV1I	GCAGC	7 cut(s)	100, 305, 344, 802, 1149, 1255, 1347
BstV2I	GAAGAC	4 cut(s)	272, 581, 653, 1917
BstX2I	RGATCY	1 cut(s)	309
BstYI	RGATCY	1 cut(s)	309
BstZI	CGGCCG	1 cut(s)	453
BtgI	CCRYGG	1 cut(s)	504
BtrI	CACGTC	1 cut(s)	1636
BtsCI	GGATG	3 cut(s)	589, 680, 1357
BtsI	GCAGTG	1 cut(s)	572
BtsIMutI	CAGTG	5 cut(s)	196, 270, 572, 1102, 1572
Cac8I	GCNNGC	9 cut(s)	97, 136, 298, 340, 826, 1160, 1671, 1839, 1843
CciI	TCATGA	2 cut(s)	1291, 1660
CfoI	GCGC	1 cut(s)	1865
Cfr10I	RCCGGY	1 cut(s)	1427
Cfr13I	GGNCC	3 cut(s)	501, 1148, 1456
Cfr42I	CCGCGG	1 cut(s)	507
CseI	GACGC	2 cut(s)	458, 1837
Csp6I	GTAC	2 cut(s)	235, 987
CviAII	CATG	6 cut(s)	21, 232, 737, 1292, 1661, 1933
CviQI	GTAC	2 cut(s)	235, 987
DdeI	CTNAG	4 cut(s)	1152, 1361, 1421, 1723
DpnI	GATC	9 cut(s)	78, 311, 848, 1094, 1197, 1224, 1618, 1644, 1659
DpnII	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
DraIII	CACNNNGTG	1 cut(s)	1120
DrdI	GACNNNNNNGTC	1 cut(s)	422
DseDI	GACNNNNNNGTC	1 cut(s)	422
EaeI	YGGCCR	3 cut(s)	430, 453, 1629
EagI	CGGCCG	1 cut(s)	453
Eam1104I	CTCTTC	3 cut(s)	32, 432, 1011
EarI	CTCTTC	3 cut(s)	32, 432, 1011
EciI	GGCGGA	4 cut(s)	325, 461, 524, 1583
Ecl136II	GAGCTC	1 cut(s)	181
EclXI	CGGCCG	1 cut(s)	453
Eco147I	AGGCCT	1 cut(s)	1721
Eco24I	GRGCYC	1 cut(s)	183
Eco31I	GGTCTC	1 cut(s)	1729
Eco47I	GGWCC	1 cut(s)	501
Eco52I	CGGCCG	1 cut(s)	453
Eco53kI	GAGCTC	1 cut(s)	181
Eco57I	CTGAAG	4 cut(s)	384, 1293, 1323, 1770
Eco88I	CYCGRG	2 cut(s)	958, 1526
EcoICRI	GAGCTC	1 cut(s)	181
EcoO109I	RGGNCCY	1 cut(s)	1148
EcoRI	GAATTC	1 cut(s)	1101
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	183
Esp3I	CGTCTC	1 cut(s)	1882
FaeI	CATG	6 cut(s)	24, 235, 740, 1295, 1664, 1936
FalI	AAGNNNNNCTT	2 cut(s)	1467, 1499
FaqI	GGGAC	4 cut(s)	389, 1015, 1337, 1968
FatI	CATG	6 cut(s)	20, 231, 736, 1291, 1660, 1932
FauI	CCCGC	3 cut(s)	323, 347, 415
FbaI	TGATCA	2 cut(s)	1092, 1222
FblI	GTMKAC	1 cut(s)	414
FokI	GGATG	3 cut(s)	596, 687, 1364
FriOI	GRGCYC	1 cut(s)	183
FspBI	CTAG	9 cut(s)	135, 242, 869, 929, 1118, 1445, 1520, 1832, 1938
GlaI	GCGC	1 cut(s)	1864
HapII	CCGG	1 cut(s)	1428
HgaI	GACGC	2 cut(s)	458, 1837
HhaI	GCGC	1 cut(s)	1865
Hin1I	GRCGYC	1 cut(s)	1904
Hin1II	CATG	6 cut(s)	24, 235, 740, 1295, 1664, 1936
Hin6I	GCGC	1 cut(s)	1863
HinP1I	GCGC	1 cut(s)	1863
HincII	GTYRAC	1 cut(s)	415
HindII	GTYRAC	1 cut(s)	415
HindIII	AAGCTT	2 cut(s)	396, 1124
HinfI	GANTC	8 cut(s)	416, 694, 759, 1003, 1012, 1357, 1583, 1922
HpaII	CCGG	1 cut(s)	1428
HphI	GGTGA	6 cut(s)	178, 493, 599, 632, 640, 1096
Hpy166II	GTNNAC	2 cut(s)	415, 744
Hpy188III	TCNNGA	7 cut(s)	350, 960, 1292, 1349, 1526, 1528, 1661
Hpy8I	GTNNAC	2 cut(s)	415, 744
Hpy99I	CGWCG	4 cut(s)	356, 425, 1637, 1831
HpyAV	CCTTC	9 cut(s)	607, 647, 747, 928, 968, 1139, 1268, 1289, 1567
HpyCH4III	ACNGT	3 cut(s)	580, 991, 1024
HpyCH4IV	ACGT	4 cut(s)	471, 1500, 1635, 1904
HpyCH4V	TGCA	7 cut(s)	4, 824, 844, 861, 1137, 1376, 1713
HpyF3I	CTNAG	4 cut(s)	1152, 1361, 1421, 1723
HpySE526I	ACGT	4 cut(s)	471, 1500, 1635, 1904
Hsp92I	GRCGYC	1 cut(s)	1904
Hsp92II	CATG	6 cut(s)	24, 235, 740, 1295, 1664, 1936
HspAI	GCGC	1 cut(s)	1863
Ksp22I	TGATCA	2 cut(s)	1092, 1222
KspI	CCGCGG	1 cut(s)	507
Kzo9I	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
LguI	GCTCTTC	1 cut(s)	32
LmnI	GCTCC	4 cut(s)	110, 782, 1188, 1850
Lsp1109I	GCAGC	7 cut(s)	100, 305, 344, 802, 1149, 1255, 1347
LweI	GCATC	1 cut(s)	351
MaeI	CTAG	9 cut(s)	135, 242, 869, 929, 1118, 1445, 1520, 1832, 1938
MaeII	ACGT	4 cut(s)	471, 1500, 1635, 1904
MaeIII	GTNAC	2 cut(s)	677, 1784
MalI	GATC	9 cut(s)	78, 311, 848, 1094, 1197, 1224, 1618, 1644, 1659
MboI	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
MfeI	CAATTG	1 cut(s)	771
MflI	RGATCY	1 cut(s)	309
MhlI	GDGCHC	3 cut(s)	183, 206, 732
MluCI	AATT	5 cut(s)	771, 1087, 1101, 1199, 1698
MlyI	GAGTC	4 cut(s)	410, 688, 997, 1931
MmeI	TCCRAC	3 cut(s)	170, 1243, 1431
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	1755
MseI	TTAA	8 cut(s)	216, 462, 495, 951, 996, 1032, 1233, 1889
MslI	CAYNNNNRTG	1 cut(s)	735
MspA1I	CMGCKG	3 cut(s)	318, 506, 1246
MspI	CCGG	1 cut(s)	1428
MunI	CAATTG	1 cut(s)	771
Mva1269I	GAATGC	2 cut(s)	254, 673
MvnI	CGCG	2 cut(s)	452, 506
NdeII	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
NheI	GCTAGC	1 cut(s)	134
NlaIII	CATG	6 cut(s)	24, 235, 740, 1295, 1664, 1936
NlaIV	GGNNCC	3 cut(s)	205, 1190, 1458
NmuCI	GTSAC	1 cut(s)	1784
NsiI	ATGCAT	1 cut(s)	6
PaeR7I	CTCGAG	2 cut(s)	958, 1526
PagI	TCATGA	2 cut(s)	1291, 1660
PceI	AGGCCT	1 cut(s)	1721
PciSI	GCTCTTC	1 cut(s)	32
PcsI	WCGNNNNNNNCGW	2 cut(s)	420, 1629
PctI	GAATGC	2 cut(s)	254, 673
PdmI	GAANNNNTTC	1 cut(s)	1755
PfeI	GAWTC	4 cut(s)	759, 1012, 1357, 1583
PflFI	GACNNNGTC	1 cut(s)	419
PleI	GAGTC	4 cut(s)	410, 688, 997, 1930
PpsI	GAGTC	4 cut(s)	410, 688, 997, 1930
Psp124BI	GAGCTC	1 cut(s)	183
Psp1406I	AACGTT	1 cut(s)	471
PspN4I	GGNNCC	3 cut(s)	205, 1190, 1458
PspPI	GGNCC	3 cut(s)	501, 1148, 1456
PsrI	GAACNNNNNNTAC	2 cut(s)	1674, 1706
PsuI	RGATCY	1 cut(s)	309
PsyI	GACNNNGTC	1 cut(s)	419
RsaI	GTAC	2 cut(s)	236, 988
RsaNI	GTAC	2 cut(s)	235, 987
RseI	CAYNNNNRTG	1 cut(s)	735
SacI	GAGCTC	1 cut(s)	183
SacII	CCGCGG	1 cut(s)	507
SalI	GTCGAC	1 cut(s)	413
SapI	GCTCTTC	1 cut(s)	32
SaqAI	TTAA	8 cut(s)	216, 462, 495, 951, 996, 1032, 1233, 1889
Sau3AI	GATC	9 cut(s)	76, 309, 846, 1092, 1195, 1222, 1616, 1642, 1657
Sau96I	GGNCC	3 cut(s)	501, 1148, 1456
SchI	GAGTC	4 cut(s)	410, 688, 997, 1931
SduI	GDGCHC	3 cut(s)	183, 206, 732
SfaNI	GCATC	1 cut(s)	351
SfcI	CTRYAG	2 cut(s)	379, 1612
Sfr274I	CTCGAG	2 cut(s)	958, 1526
Sfr303I	CCGCGG	1 cut(s)	507
SgrBI	CCGCGG	1 cut(s)	507
SinI	GGWCC	1 cut(s)	501
SlaI	CTCGAG	2 cut(s)	958, 1526
SmiMI	CAYNNNNRTG	1 cut(s)	735
SmlI	CTYRAG	5 cut(s)	682, 907, 958, 1526, 1651
SmoI	CTYRAG	5 cut(s)	682, 907, 958, 1526, 1651
Sse9I	AATT	5 cut(s)	771, 1087, 1101, 1199, 1698
SseBI	AGGCCT	1 cut(s)	1721
SspMI	CTAG	9 cut(s)	135, 242, 869, 929, 1118, 1445, 1520, 1832, 1938
SstI	GAGCTC	1 cut(s)	183
StuI	AGGCCT	1 cut(s)	1721
TaaI	ACNGT	3 cut(s)	580, 991, 1024
TaiI	ACGT	4 cut(s)	474, 1503, 1638, 1907
TaqI	TCGA	7 cut(s)	351, 414, 692, 959, 1194, 1209, 1527
TasI	AATT	5 cut(s)	771, 1087, 1101, 1199, 1698
TauI	GCSGC	6 cut(s)	296, 303, 432, 446, 455, 509
TfiI	GAWTC	4 cut(s)	759, 1012, 1357, 1583
Tru1I	TTAA	8 cut(s)	216, 462, 495, 951, 996, 1032, 1233, 1889
Tru9I	TTAA	8 cut(s)	216, 462, 495, 951, 996, 1032, 1233, 1889
TscAI	CASTG	5 cut(s)	203, 277, 572, 1102, 1579
TseFI	GTSAC	1 cut(s)	1784
TseI	GCWGC	7 cut(s)	113, 318, 357, 815, 1137, 1243, 1335
Tsp45I	GTSAC	1 cut(s)	1784
TspDTI	ATGAA	7 cut(s)	25, 31, 130, 936, 1185, 1280, 1308
TspGWI	ACGGA	1 cut(s)	409
TspRI	CASTG	5 cut(s)	203, 277, 572, 1102, 1579
Tth111I	GACNNNGTC	1 cut(s)	419
VpaK11BI	GGWCC	1 cut(s)	501
XapI	RAATTY	2 cut(s)	1087, 1101
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1939
XhoI	CTCGAG	2 cut(s)	958, 1526
XmiI	GTMKAC	1 cut(s)	414
XmnI	GAANNNNTTC	1 cut(s)	1755
XspI	CTAG	9 cut(s)	135, 242, 869, 929, 1118, 1445, 1520, 1832, 1938
ZraI	GACGTC	1 cut(s)	1905
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.