Rorug01G0488900

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001832
Physical Location & Seq
Forward (+)
57418302 .. 57431106
12805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0488900.1

Sequence Viewer

Length: 4317 bp
ATGGATGAGCTCAGCCCTACTCCTGCCTCTTCGTGGCGTCCCAGCCAGCTGGTCTTTACTCCATATTCCACGCAGGCTGAAGCTGCGAGCAACAAGTCCCAACCTTTACGCGTTATCAGAAGACCGTTTGTGGCAAGATTAACTAAGGACATAGTTGAAACATACCGAATCTGCAATCCACAGTTTAAGTATTCAGAAGAACTGAATCCAAAGAGATATTTGACCAGCCCGTCAATTGGAGTACTCAATGAAGGCCACGATAATGTTAACTCGGATCTTATTCTGACAGTAAACTTTGTCTTGGTCAATTTAGACACACATCGAAGATACATCGTCAAAGATGTTCTTGGCCATGGGACATTTGGCCAGGTTGCTAAATGCTGGGTTCCAGAGACTAACAGTTTTGTTGCTGTGAAGATCATTAAAAATCAACCAGCATACTATCAACAGGCACTGGTTGAAGTATCCATTTTGACAACGTTAAACAAGAAATATGATCCTGAGGACAAGCATCACATTGTTCGTATTTATGATTACTTTGTATATCATCGTCATTTGTGCATTTGTTTTGAACTGCTTGATACTAATCTGTATGAGCTTATCAAGATAAATCAATTCAGGGGGCTGTCCTTGACCATTGTCCAATTGTTCTCTAAACAAATTCTACATGGGTTAGCCCTGTTAAAGGATGCTGGGATAATTCACTGTGATCTGAAGCCAGAAAACATTCTTTTATGCACAAGTGTTAAACCAGCAGAAATTAAAATTATTGACTTTGGATCTGCATGCATGGAAGATCGAACTGTTTACTCCTACATTCAGAGTCGTTACTACAGGTCCCCTGAAGTCCTGCTTGGATATCAATATACTACTGCTATCGATATGTGGTCTTTTGGATGCATAGTTGCTGAACTGTTTCTAGGATTGCCATTATTTCCAGGAGCTTCAGAATTTGACCTCTTAAGGAGAATGATTGAAATACTTGGAGGGCAACCCCCTGATTATGTACTTAAGGAGGCAAAAAACACAAGCAAGTTCTTTAAGTGCATTCGGAGTGTGCACAGTATGGAGAATGGAGAATTTTCTGCAACTGGCAAAAGTTCTTATCAAGCTTTAACAGAGGAAGAATATGAAGCTAGAGAATTGAAAAGACCATCAATTGGGAAGGAGTATTTTAATCATATGAACCTTGAAGAAATTGTTACAAAATATCCATATCGGAAGAACTTACCTAAGGAAGATATGATTAAAGAAAGTCAGATACGATTAGCTTTGGTTGATTTTTTGAGGGGCCTTGTTGAGTTTGATCCTTCAAAACGCTGGTCACCTTTTCAGGCTTCAAAACACCCTTTTGTTACGGGGGAACCTTTTACTTGCCCTTATCAACCTCCATCAGAGACTCCTCGCATGCCTGTTGCTCAAAACATAAGGGTAGATCATCATCCTGGTGGTGGGCATTGGTTTGCTGCGGGTCTCTCTCCTAATATTCCAGGCAGAAACAGGGTCTCCATGCATAGCAGCCCACATTTTCAGGTGGTGCCGCCATATGCACATGCTAATAGCTATGGTAGTGTAGGAAGCCATGGCAGTTATAATGATGGTACTGGACTTGGAAGCAGCTATGGGAGCTATGGAGATACTGGTAATATGTTTGCTTATTATTCACCAGTTGGTCCATCTGGGATGAACATGCATGCTCAAGGCAATGCGTCAATGCTTGGAAGTAGTCCCGATGCAAGACGTAGGATTATCCCATATTCACATGGAAATGGACTTGGTATGAGCCCTTCAGCAGGAAGTTTTGCTCCTCTGCCCCTAGGCACTAGTCCTTCACAATTTACTCCACCAAGTTCCTACGGTCAAGTTTCTGCTGGCTCCCCGGGACATTATGGTCCAACTTCTCCAGCAAGAGGCAGTTGTCACGGATCACCTTTAGGAAAGATGGCTGCAGTTAGTCAGTTCAATAGAAGAAAAAGTTGGGGATATCCCGGAGGTTCTCAAACTCAGGAGAGTTCGTCTTCTCATTGGCAAGGGCAAACTACAGATGGCATCAGTTCTGGCAAAGCTGAGGGGAATTCTCAAGTAGTTGGTGGTTCCCCATCACATCTGCCTCTAAGTTCTAGTGCTGGAAGCTGGAAACAGCAGCGAGGAGGCAGTGGAAGTTCTGCTGGCTACTTAGCCACTCCAAACATGCCAAGCTCCTTCAAGGTTGGTTCCAATATCCCAAAGACCACAGGAGTGGCTCATGACAATTCGGAGGCTAGCCTGTCATTGCCTGATCCTGGGGATTGGGATCCCAATTATAGCGATGAACTTCTTCTGCAAGAAGATGGTTCGGATACAAGCTGCTTTACTACTGAGTTCAGCAATGGCATGCATCTTAATTCGGCAGAAACTTTGGGTGGGGTTGGAAGATTCAGCAGCATGTCAAGTACAAGCTCCGGTCTTTCCTTACACAGACAAAGTGGCCCAATGCAATCATTCTCACATGCAGAGGTGGGTAGCTCTCCAACTGGTGAACCACTTGCTGGATATGTGCGTTTGTCAAAACCTTCTCATTTCATGCCTCATATATCTCAAAATTCTCCTAGCCGTTTGGGCCAGCAATCTCAGCGGTTCAATCACGGAAGACCGAGCAATTCTCGGGGTAATGATTGGAATCATATGAAGGTGCAGCCCCCTCCTCCCAATTTTAACTCTGGAGGCCCACATTCTCCAGGAAATAGCTCATTCAGCAACGGCATGTCATGGGTGCACGCAACAAGACGCCATATGATGGCCACAGCTGCTGCTGCTGCTGCTGTTGGGCTTAGTAGTACGGGGAAGAGGCTCTTTAGTTCTTCCTTGTATTATTCAGATATCACAGATAAGCTCTTTTATCTCAATGATCATGGCTTTGTAAATTGTCAATTGTCTTCAACAAAGAATGTTGTCACTGCCAGAAAGTCGTCTTCCAATTACAGTAATTCCAGATTTCCATCTTCAAATAGACATCAAGATCAGTCCATCAGGGCTTTAAAAGAGCATGCAGATACCGCATCTGCTCCTTCATCAACCACACATCAATTGCTAGAAGATTACAACGACTTGGAGGAGGAAAGCTCCGATCTTGAATATTCAGTGGAGGCGCTTCTTCTGCTACAGAAGTCTATGCTGGAAAAGCAGTGGAATCTTTCCTTTGAGAAAAAACTGTCAAGAAGAAAAATAACTGATAAGAAGATACCTGTCACTTGTTCTGGGGTATCTGCTCGGCAGAGGAGGATGAGTACTAAGAGGAAAACTTTCAGCAAAAATACTTTAGAAATGCAACCTAGCATCAACAAGCATCTCAGAGCAAGTATCAGTCCGAGGCTGCTGCAGAATCGTATAAAGGGTTATGTGAAGGGCGTTACGAGTGAGCATTTGCTGACTCACACTGAGGTCGTACGCCTGTCAAAGAAAATTAAGATCGGGCTTTCAGTGGAGGAGCATAAATCAAGACTGAAGGAGAAACTGGGTTGTGAGCCCTCTGACGAACAACTCGCAACTTCATTGAGGATTTCCCGCACTGAACTACAGTCAAAGTTAATCCAATGTTCTTTGGCAAGAGAGAAGCTGACAATGAGCAATGTTCGTCTGGTCATGTCCATTGCCCAAAGATATGATAACATGGGTGCTGAAATGGCTGACCTTGTCCAGGGTGGCTTAATTGGACTACTACGCGGGATTGAGAAATTTGATTCATCAAAGGGGTTCAAAATTTCTACTTATGTTTACTGGTGGATACGTCAGGGTGTTTCAAGAGCACTGGTTGAAAATTCAAGAACCTTAAGACTGCCTACACATTTGCATGAGAGGTTAGGGTTAATCAGAAATGCAAAAGTTAGATTGGAAGAGAAAGGAATAACTCCGTCTATTGATAGGATTGCAGAAAGCCTGAATATGTCCAAAAAGAAAGTTAAGAATGCTACTGAGGCAATCAGTAAGGTTTTTTCGCTTGATAGAGATGCATTCCCCTCTTTGAATGGCCTTCCAGGAGAAACTCATCATAGTTACATTGCTGATAATCGCCATGAGAACATCCCATGGCATGGGGTGGATGAGTTGGCACTCAAGGATGAAGTAAACAAGCTCATAAATATGATGCTTGTGGAGCGTGAGCGAGAGATCATACGGCTTTACTATGGTCTGGATAATGAATGTCTTACATGGGAGGACATTAGTAAACGTATAGGATTGTCCAGAGAAAGAGTTAGGCAAGTCGGACTGGTTGCTCTAGAGAAACTGAAACATGGTGCGAGGAAAAAAGAGATGGAGGCCATGCTAGTCCAACATTAA

Protein Analysis

1438

Amino Acids

159.89

Weight (kDa)

9.16

Isoelectric Point (pI)

50.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 110 - 452 4.2e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 113 - 324 5.4e-21 Protein tyrosine and serine/threonine kinase
Sigma70_r2 PF04542 1201 - 1268 1.1e-16 Sigma-70 region 2
Sigma70_r3 PF04539 1278 - 1349 4.1e-12 Sigma-70 region 3
Sigma70_r4 PF04545 1376 - 1424 1.4e-09 Sigma-70, region 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1593
AasI GACNNNNNNGTC 2 cut(s) 229, 1890
AccB1I GGYRCC 1 cut(s) 1537
AccB7I CCANNNNNTGG 4 cut(s) 1160, 2528, 2776, 4070
AccII CGCG 2 cut(s) 111, 3702
AciI CCGC 6 cut(s) 1469, 1541, 2614, 3036, 3544, 3702
AclI AACGTT 1 cut(s) 479
AclWI GGATC 8 cut(s) 282, 491, 787, 1301, 1933, 2273, 2288, 2301
AcoI YGGCCR 3 cut(s) 349, 364, 2778
AcsI RAATTY 8 cut(s) 660, 950, 1079, 2074, 2581, 3713, 3738, 3796
AcuI CTGAAG 6 cut(s) 99, 734, 864, 930, 1773, 3503
AcyI GRCGYC 2 cut(s) 37, 2767
AfaI GTAC 7 cut(s) 243, 1008, 1603, 2434, 2818, 3268, 3426
AflII CTTAAG 3 cut(s) 961, 1010, 3808
AflIII ACRYGT 1 cut(s) 109
AhlI ACTAGT 1 cut(s) 1823
AjnI CCWGG 8 cut(s) 366, 937, 1444, 1489, 2281, 2716, 3675, 4012
AjuI GAANNNNNNNTTGG 4 cut(s) 837, 869, 1960, 1992
AleI CACNNNNGTG 1 cut(s) 2237
Alw21I GWGCWC 4 cut(s) 12, 1062, 2757, 3787
Alw26I GTCTC 4 cut(s) 386, 1391, 1478, 1510
Alw44I GTGCAC 2 cut(s) 1058, 2753
AlwI GGATC 8 cut(s) 282, 491, 787, 1301, 1933, 2273, 2288, 2301
AlwNI CAGNNNCTG 2 cut(s) 454, 2789
Ama87I CYCGRG 2 cut(s) 1879, 2643
ApaLI GTGCAC 2 cut(s) 1058, 2753
ApoI RAATTY 8 cut(s) 660, 950, 1079, 2074, 2581, 3713, 3738, 3796
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
Asp700I GAANNNNTTC 4 cut(s) 726, 915, 2316, 3281
AspA2I CCTAGG 1 cut(s) 1816
AspLEI GCGC 1 cut(s) 3130
AspS9I GGNCC 7 cut(s) 837, 1291, 1673, 1892, 2468, 2599, 2705
AsuC2I CCSGG 3 cut(s) 1880, 1881, 1989
AsuHPI GGTGA 4 cut(s) 1317, 1656, 1920, 2528
AsuNHI GCTAGC 1 cut(s) 2261
AvaI CYCGRG 2 cut(s) 1879, 2643
AvaII GGWCC 3 cut(s) 837, 1673, 1892
AvrII CCTAGG 1 cut(s) 1816
AxyI CCTNAGG 2 cut(s) 501, 1233
BaeGI GKGCMC 2 cut(s) 1062, 2757
BalI TGGCCA 3 cut(s) 351, 366, 2780
BamHI GGATCC 1 cut(s) 2293
BanI GGYRCC 1 cut(s) 1537
BanII GRGCYC 3 cut(s) 12, 1787, 3507
BarI GAAGNNNNNNTAC 2 cut(s) 1771, 1803
BbsI GAAGAC 5 cut(s) 127, 2010, 2635, 2907, 2943
Bbv12I GWGCWC 4 cut(s) 12, 1062, 2757, 3787
BbvCI CCTCAGC 1 cut(s) 2067
BceAI ACGGC 3 cut(s) 2577, 2755, 4169
BcgI CGANNNNNNTGC 2 cut(s) 3337, 3371
BciT130I CCWGG 8 cut(s) 368, 939, 1446, 1491, 2283, 2718, 3677, 4014
BciVI GTATCC 3 cut(s) 475, 2332, 3756
BclI TGATCA 1 cut(s) 2887
BcnI CCSGG 3 cut(s) 1880, 1881, 1989
BcoDI GTCTC 4 cut(s) 386, 1391, 1478, 1510
BcuI ACTAGT 1 cut(s) 1823
BfmI CTRYAG 6 cut(s) 832, 1947, 2040, 3140, 3356, 3554
BfoI RGCGCY 1 cut(s) 3131
BfrI CTTAAG 3 cut(s) 961, 1010, 3808
BfuI GTATCC 3 cut(s) 475, 2332, 3756
BglI GCCNNNNNGGC 1 cut(s) 2598
BlnI CCTAGG 1 cut(s) 1816
BlpI GCTNAGC 1 cut(s) 11
BmcAI AGTACT 2 cut(s) 243, 3268
Bme18I GGWCC 3 cut(s) 837, 1673, 1892
BmeT110I CYCGRG 2 cut(s) 1879, 2643
BmgT120I GGNCC 7 cut(s) 837, 1291, 1673, 1892, 2468, 2599, 2705
BmiI GGNNCC 9 cut(s) 387, 839, 1292, 1365, 1539, 1876, 2095, 2215, 2295
BmrI ACTGGG 1 cut(s) 3503
BmtI GCTAGC 1 cut(s) 2265
BmuI ACTGGG 1 cut(s) 3503
BoxI GACNNNNGTC 1 cut(s) 638
BpiI GAAGAC 5 cut(s) 127, 2010, 2635, 2907, 2943
BplI GAGNNNNNCTC 4 cut(s) 2626, 2658, 3086, 3118
BpmI CTGGAG 3 cut(s) 1887, 2700, 2721
Bpu10I CCTNAGC 1 cut(s) 2067
Bpu1102I GCTNAGC 1 cut(s) 11
BpuEI CTTGAG 3 cut(s) 1683, 2064, 4076
BpuMI CCSGG 3 cut(s) 1880, 1881, 1989
Bsa29I ATCGAT 1 cut(s) 879
BsaBI GATNNNNATC 5 cut(s) 585, 1440, 2292, 2658, 2977
BsaHI GRCGYC 2 cut(s) 37, 2767
BsaI GGTCTC 2 cut(s) 1478, 1510
BsaJI CCNNGG 9 cut(s) 352, 1582, 1816, 1878, 1879, 2282, 3347, 3676, 4064
BsaWI WCCGGW 1 cut(s) 2441
BsaXI ACNNNNNCTCC 6 cut(s) 1490, 1520, 2000, 2030, 3250, 3280
Bse21I CCTNAGG 2 cut(s) 501, 1233
Bse3DI GCAATG 6 cut(s) 1711, 2270, 2374, 3613, 3627, 4035
Bse8I GATNNNNATC 5 cut(s) 585, 1440, 2292, 2658, 2977
BseBI CCWGG 8 cut(s) 368, 939, 1446, 1491, 2283, 2718, 3677, 4014
BseCI ATCGAT 1 cut(s) 879
BseDI CCNNGG 9 cut(s) 352, 1582, 1816, 1878, 1879, 2282, 3347, 3676, 4064
BseGI GGATG 9 cut(s) 10, 694, 902, 1441, 1689, 3267, 4059, 4084, 4102
BseJI GATNNNNATC 5 cut(s) 585, 1440, 2292, 2658, 2977
BseMI GCAATG 6 cut(s) 1711, 2270, 2374, 3613, 3627, 4035
BseMII CTCAG 9 cut(s) 25, 492, 2018, 2058, 2349, 2624, 3343, 3408, 3942
BseRI GAGGAG 7 cut(s) 1392, 1797, 2163, 2673, 3107, 3271, 3479
BseSI GKGCMC 2 cut(s) 1062, 2757
BseYI CCCAGC 3 cut(s) 41, 381, 692
BsgI GTGCAG 1 cut(s) 2693
Bsh1236I CGCG 2 cut(s) 111, 3702
BshNI GGYRCC 1 cut(s) 1537
BshVI ATCGAT 1 cut(s) 879
BsiHKAI GWGCWC 4 cut(s) 12, 1062, 2757, 3787
BsiHKCI CYCGRG 2 cut(s) 1879, 2643
BsiSI CCGG 3 cut(s) 1880, 1989, 2442
BsiWI CGTACG 1 cut(s) 3424
BslFI GGGAC 6 cut(s) 24, 82, 370, 823, 1713, 1896
BsmAI GTCTC 4 cut(s) 386, 1391, 1478, 1510
BsmFI GGGAC 6 cut(s) 24, 82, 370, 823, 1713, 1896
BsmI GAATGC 3 cut(s) 1047, 3949, 3989
Bso31I GGTCTC 2 cut(s) 1478, 1510
BsoBI CYCGRG 2 cut(s) 1879, 2643
Bsp1286I GDGCHC 6 cut(s) 12, 1062, 1787, 2757, 3507, 3787
Bsp1720I GCTNAGC 1 cut(s) 11
Bsp19I CCATGG 3 cut(s) 352, 1582, 4064
BspACI CCGC 6 cut(s) 1469, 1541, 2614, 3036, 3544, 3702
BspCNI CTCAG 9 cut(s) 24, 493, 2017, 2059, 2350, 2623, 3342, 3409, 3943
BspDI ATCGAT 1 cut(s) 879
BspFNI CGCG 2 cut(s) 111, 3702
BspHI TCATGA 1 cut(s) 2245
BspLI GGNNCC 9 cut(s) 387, 839, 1292, 1365, 1539, 1876, 2095, 2215, 2295
BspMAI CTGCAG 2 cut(s) 1951, 3360
BspOI GCTAGC 1 cut(s) 2265
BspPI GGATC 8 cut(s) 282, 491, 787, 1301, 1933, 2273, 2288, 2301
BspT107I GGYRCC 1 cut(s) 1537
BspTI CTTAAG 3 cut(s) 961, 1010, 3808
BspTNI GGTCTC 2 cut(s) 1478, 1510
BsrDI GCAATG 6 cut(s) 1711, 2270, 2374, 3613, 3627, 4035
BssECI CCNNGG 9 cut(s) 352, 1582, 1816, 1878, 1879, 2282, 3347, 3676, 4064
BssNI GRCGYC 2 cut(s) 37, 2767
BssT1I CCWWGG 4 cut(s) 352, 1582, 1816, 4064
Bst2UI CCWGG 8 cut(s) 368, 939, 1446, 1491, 2283, 2718, 3677, 4014
Bst6I CTCTTC 3 cut(s) 34, 2819, 3867
BstACI GRCGYC 2 cut(s) 37, 2767
BstAFI CTTAAG 3 cut(s) 961, 1010, 3808
BstDSI CCRYGG 3 cut(s) 352, 1582, 4064
BstEII GGTNACC 1 cut(s) 1323
BstENI CCTNNNNNAGG 3 cut(s) 683, 1791, 3674
BstF5I GGATG 9 cut(s) 10, 694, 902, 1441, 1689, 3267, 4059, 4084, 4102
BstFNI CGCG 2 cut(s) 111, 3702
BstH2I RGCGCY 1 cut(s) 3131
BstHHI GCGC 1 cut(s) 3130
BstMAI GTCTC 4 cut(s) 386, 1391, 1478, 1510
BstNI CCWGG 8 cut(s) 368, 939, 1446, 1491, 2283, 2718, 3677, 4014
BstPAI GACNNNNGTC 1 cut(s) 638
BstPI GGTNACC 1 cut(s) 1323
BstSFI CTRYAG 6 cut(s) 832, 1947, 2040, 3140, 3356, 3554
BstSLI GKGCMC 2 cut(s) 1062, 2757
BstUI CGCG 2 cut(s) 111, 3702
BstV2I GAAGAC 5 cut(s) 127, 2010, 2635, 2907, 2943
BstX2I RGATCY 3 cut(s) 274, 779, 2293
BstXI CCANNNNNNTGG 2 cut(s) 49, 2239
BstYI RGATCY 3 cut(s) 274, 779, 2293
Bsu15I ATCGAT 1 cut(s) 879
Bsu36I CCTNAGG 2 cut(s) 501, 1233
BsuI GTATCC 3 cut(s) 475, 2332, 3756
BsuTUI ATCGAT 1 cut(s) 879
BtgI CCRYGG 3 cut(s) 352, 1582, 4064
BtgZI GCGATG 1 cut(s) 2322
BtsCI GGATG 9 cut(s) 10, 694, 902, 1441, 1689, 3267, 4059, 4084, 4102
BtsI GCAGTG 3 cut(s) 2161, 2934, 3170
CaiI CAGNNNCTG 2 cut(s) 454, 2789
CciI TCATGA 1 cut(s) 2245
CfoI GCGC 1 cut(s) 3130
Cfr13I GGNCC 7 cut(s) 837, 1291, 1673, 1892, 2468, 2599, 2705
Cfr9I CCCGGG 1 cut(s) 1879
ClaI ATCGAT 1 cut(s) 879
CseI GACGC 3 cut(s) 26, 1698, 2775
Csp6I GTAC 7 cut(s) 242, 1007, 1602, 2433, 2817, 3267, 3425
CviQI GTAC 7 cut(s) 242, 1007, 1602, 2433, 2817, 3267, 3425
DraI TTTAAA 1 cut(s) 3018
DrdI GACNNNNNNGTC 2 cut(s) 229, 1890
DseDI GACNNNNNNGTC 2 cut(s) 229, 1890
EaeI YGGCCR 3 cut(s) 349, 364, 2778
Eam1104I CTCTTC 3 cut(s) 34, 2819, 3867
EarI CTCTTC 3 cut(s) 34, 2819, 3867
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 4 cut(s) 352, 1582, 1816, 4064
Eco24I GRGCYC 3 cut(s) 12, 1787, 3507
Eco31I GGTCTC 2 cut(s) 1478, 1510
Eco32I GATATC 3 cut(s) 860, 1985, 2860
Eco47I GGWCC 3 cut(s) 837, 1673, 1892
Eco53kI GAGCTC 1 cut(s) 10
Eco57I CTGAAG 6 cut(s) 99, 734, 864, 930, 1773, 3503
Eco81I CCTNAGG 2 cut(s) 501, 1233
Eco88I CYCGRG 2 cut(s) 1879, 2643
Eco91I GGTNACC 1 cut(s) 1323
EcoICRI GAGCTC 1 cut(s) 10
EcoNI CCTNNNNNAGG 3 cut(s) 683, 1791, 3674
EcoO109I RGGNCCY 2 cut(s) 837, 1291
EcoO65I GGTNACC 1 cut(s) 1323
EcoRI GAATTC 1 cut(s) 2074
EcoRII CCWGG 8 cut(s) 366, 937, 1444, 1489, 2281, 2716, 3675, 4012
EcoRV GATATC 3 cut(s) 860, 1985, 2860
EcoT14I CCWWGG 4 cut(s) 352, 1582, 1816, 4064
EcoT22I ATGCAT 6 cut(s) 791, 902, 1515, 1695, 2379, 3991
EcoT38I GRGCYC 3 cut(s) 12, 1787, 3507
ErhI CCWWGG 4 cut(s) 352, 1582, 1816, 4064
FalI AAGNNNNNCTT 6 cut(s) 330, 362, 837, 869, 2816, 2848
FaqI GGGAC 6 cut(s) 24, 82, 370, 823, 1713, 1896
FauI CCCGC 3 cut(s) 1462, 3551, 3695
FauNDI CATATG 4 cut(s) 1182, 1546, 2664, 2772
FbaI TGATCA 1 cut(s) 2887
FokI GGATG 9 cut(s) 17, 701, 909, 1428, 1696, 3274, 4046, 4091, 4109
FriOI GRGCYC 3 cut(s) 12, 1787, 3507
GlaI GCGC 1 cut(s) 3129
GsaI CCCAGC 3 cut(s) 45, 385, 696
GsuI CTGGAG 3 cut(s) 1887, 2700, 2721
HaeII RGCGCY 1 cut(s) 3131
HapII CCGG 3 cut(s) 1880, 1989, 2442
HgaI GACGC 3 cut(s) 26, 1698, 2775
HhaI GCGC 1 cut(s) 3130
Hin1I GRCGYC 2 cut(s) 37, 2767
Hin6I GCGC 1 cut(s) 3128
HinP1I GCGC 1 cut(s) 3128
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 1110
HpaI GTTAAC 1 cut(s) 268
HpaII CCGG 3 cut(s) 1880, 1989, 2442
HphI GGTGA 4 cut(s) 1317, 1656, 1920, 2528
Hpy166II GTNNAC 9 cut(s) 268, 292, 808, 1060, 2519, 2755, 3754, 4105, 4205
Hpy8I GTNNAC 9 cut(s) 268, 292, 808, 1060, 2519, 2755, 3754, 4105, 4205
HpyCH4IV ACGT 4 cut(s) 479, 1741, 3766, 4207
HpySE526I ACGT 4 cut(s) 479, 1741, 3766, 4207
Hsp92I GRCGYC 2 cut(s) 37, 2767
HspAI GCGC 1 cut(s) 3128
Ksp22I TGATCA 1 cut(s) 2887
KspAI GTTAAC 1 cut(s) 268
MaeII ACGT 4 cut(s) 479, 1741, 3766, 4207
MaeIII GTNAC 9 cut(s) 827, 1201, 1323, 1354, 1919, 2932, 3226, 3388, 4031
MfeI CAATTG 5 cut(s) 234, 644, 1158, 2909, 3065
MflI RGATCY 3 cut(s) 274, 779, 2293
MhlI GDGCHC 6 cut(s) 12, 1062, 1787, 2757, 3507, 3787
MlsI TGGCCA 3 cut(s) 351, 366, 2780
MluI ACGCGT 1 cut(s) 109
MluNI TGGCCA 3 cut(s) 351, 366, 2780
MlyI GAGTC 3 cut(s) 832, 1393, 3403
MmeI TCCRAC 4 cut(s) 1919, 2389, 2534, 4222
Mox20I TGGCCA 3 cut(s) 351, 366, 2780
Mph1103I ATGCAT 6 cut(s) 791, 902, 1515, 1695, 2379, 3991
MroXI GAANNNNTTC 4 cut(s) 726, 915, 2316, 3281
MscI TGGCCA 3 cut(s) 351, 366, 2780
MslI CAYNNNNRTG 6 cut(s) 261, 1446, 1767, 2237, 3828, 4118
Msp20I TGGCCA 3 cut(s) 351, 366, 2780
MspA1I CMGCKG 3 cut(s) 49, 2614, 2786
MspCI CTTAAG 3 cut(s) 961, 1010, 3808
MspI CCGG 3 cut(s) 1880, 1989, 2442
MunI CAATTG 5 cut(s) 234, 644, 1158, 2909, 3065
Mva1269I GAATGC 3 cut(s) 1047, 3949, 3989
MvaI CCWGG 8 cut(s) 368, 939, 1446, 1491, 2283, 2718, 3677, 4014
MvnI CGCG 2 cut(s) 111, 3702
NciI CCSGG 3 cut(s) 1880, 1881, 1989
NcoI CCATGG 3 cut(s) 352, 1582, 4064
NdeI CATATG 4 cut(s) 1182, 1546, 2664, 2772
NheI GCTAGC 1 cut(s) 2261
NlaIV GGNNCC 9 cut(s) 387, 839, 1292, 1365, 1539, 1876, 2095, 2215, 2295
NmeAIII GCCGAG 1 cut(s) 3229
NmuCI GTSAC 4 cut(s) 1323, 1919, 2932, 3226
NsiI ATGCAT 6 cut(s) 791, 902, 1515, 1695, 2379, 3991
OliI CACNNNNGTG 1 cut(s) 2237
PaeI GCATGC 5 cut(s) 789, 1411, 1697, 2377, 3029
PagI TCATGA 1 cut(s) 2245
PctI GAATGC 3 cut(s) 1047, 3949, 3989
PdmI GAANNNNTTC 4 cut(s) 726, 915, 2316, 3281
PfeI GAWTC 7 cut(s) 168, 205, 2415, 2659, 3169, 3361, 3719
Pfl23II CGTACG 1 cut(s) 3424
PflFI GACNNNGTC 1 cut(s) 3671
PflMI CCANNNNNTGG 4 cut(s) 1160, 2528, 2776, 4070
PfoI TCCNGGA 4 cut(s) 937, 1987, 2716, 4012
PleI GAGTC 3 cut(s) 831, 1393, 3403
PpsI GAGTC 3 cut(s) 831, 1393, 3403
PpuMI RGGWCCY 1 cut(s) 837
PshAI GACNNNNGTC 1 cut(s) 638
PsiI TTATAA 1 cut(s) 1593
Psp124BI GAGCTC 1 cut(s) 12
Psp1406I AACGTT 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 837
Psp6I CCWGG 8 cut(s) 366, 937, 1444, 1489, 2281, 2716, 3675, 4012
PspEI GGTNACC 1 cut(s) 1323
PspFI CCCAGC 3 cut(s) 41, 381, 692
PspGI CCWGG 8 cut(s) 366, 937, 1444, 1489, 2281, 2716, 3675, 4012
PspLI CGTACG 1 cut(s) 3424
PspN4I GGNNCC 9 cut(s) 387, 839, 1292, 1365, 1539, 1876, 2095, 2215, 2295
PspPI GGNCC 7 cut(s) 837, 1291, 1673, 1892, 2468, 2599, 2705
PspPPI RGGWCCY 1 cut(s) 837
PstI CTGCAG 2 cut(s) 1951, 3360
PstNI CAGNNNCTG 2 cut(s) 454, 2789
PsuI RGATCY 3 cut(s) 274, 779, 2293
PsyI GACNNNGTC 1 cut(s) 3671
PvuII CAGCTG 2 cut(s) 49, 2786
RsaI GTAC 7 cut(s) 243, 1008, 1603, 2434, 2818, 3268, 3426
RsaNI GTAC 7 cut(s) 242, 1007, 1602, 2433, 2817, 3267, 3425
RseI CAYNNNNRTG 6 cut(s) 261, 1446, 1767, 2237, 3828, 4118
SacI GAGCTC 1 cut(s) 12
Sau96I GGNCC 7 cut(s) 837, 1291, 1673, 1892, 2468, 2599, 2705
ScaI AGTACT 2 cut(s) 243, 3268
SchI GAGTC 3 cut(s) 832, 1393, 3403
SduI GDGCHC 6 cut(s) 12, 1062, 1787, 2757, 3507, 3787
SfcI CTRYAG 6 cut(s) 832, 1947, 2040, 3140, 3356, 3554
SinI GGWCC 3 cut(s) 837, 1673, 1892
SmaI CCCGGG 1 cut(s) 1881
SmiMI CAYNNNNRTG 6 cut(s) 261, 1446, 1767, 2237, 3828, 4118
SmlI CTYRAG 6 cut(s) 961, 1010, 1698, 2079, 3808, 4091
SmoI CTYRAG 6 cut(s) 961, 1010, 1698, 2079, 3808, 4091
SpeI ACTAGT 1 cut(s) 1823
SphI GCATGC 5 cut(s) 789, 1411, 1697, 2377, 3029
SsiI CCGC 6 cut(s) 1469, 1541, 2614, 3036, 3544, 3702
SspI AATATT 2 cut(s) 1486, 3116
SstI GAGCTC 1 cut(s) 12
StyI CCWWGG 4 cut(s) 352, 1582, 1816, 4064
TaiI ACGT 4 cut(s) 482, 1744, 3769, 4210
TaqI TCGA 3 cut(s) 322, 799, 879
TaqII GACCGA 1 cut(s) 2647
TatI WGTACW 4 cut(s) 241, 1006, 2432, 3266
TauI GCSGC 1 cut(s) 1543
TfiI GAWTC 7 cut(s) 168, 205, 2415, 2659, 3169, 3361, 3719
TseFI GTSAC 4 cut(s) 1323, 1919, 2932, 3226
Tsp45I GTSAC 4 cut(s) 1323, 1919, 2932, 3226
TspGWI ACGGA 3 cut(s) 1938, 2640, 3879
TspMI CCCGGG 1 cut(s) 1879
Tth111I GACNNNGTC 1 cut(s) 3671
Van91I CCANNNNNTGG 4 cut(s) 1160, 2528, 2776, 4070
Vha464I CTTAAG 3 cut(s) 961, 1010, 3808
VneI GTGCAC 2 cut(s) 1058, 2753
VpaK11BI GGWCC 3 cut(s) 837, 1673, 1892
XagI CCTNNNNNAGG 3 cut(s) 683, 1791, 3674
XapI RAATTY 8 cut(s) 660, 950, 1079, 2074, 2581, 3713, 3738, 3796
XbaI TCTAGA 1 cut(s) 4255
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XmaI CCCGGG 1 cut(s) 1879
XmaJI CCTAGG 1 cut(s) 1816
XmnI GAANNNNTTC 4 cut(s) 726, 915, 2316, 3281
ZrmI AGTACT 2 cut(s) 243, 3268
Zsp2I ATGCAT 6 cut(s) 791, 902, 1515, 1695, 2379, 3991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.