Rh2DG664300

cysteine-rich receptor-like protein kinase 43

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001832
Physical Location & Seq
Forward (+)
88982951 .. 88984553
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG664300.1

Sequence Viewer

Length: 978 bp
ATGTTACCTTCAACAAGGTGGTGGCTGAGATGGATAAAAAAAATGCTCCGTAACAACTTTGGGACTGCGGAAGAGGGTGAGCCTCCTGACCGGCTTTATATGTTGGCTCAGTGCATGACAGATCTTTCGGTCAATGAATGTAAGACGTGCTATGATTTGAGTAATCATGCCTTGGCTGGTTGCCTTCCTAACACAGGAGGTCGTGCTTTATTTGATGGTTGCTTTATAAGGGCTGAAAATTACAGCTTCTTTGATGAGACATTCCAAGATAAAGATTTCAAGCATTGCAGCGACAATGCCGAGTTCAATCAGAAATTTAACCAAACACCAGAGAAAGTGTTCAAAAAACTTGTGAAGTTGGCTGAGCATAATGATGGATTTGCAGTCGCCAAAGATAGCTCAAATGGTTTTAATGGTTTTACAGTGTTTGCTATGGCTAATTGCTGGAGGAGTCTAGACAGTAGTAAGTGTTCTTCATGTGTAGAAAATGCAGCCAACCGTGCAATTTCATGCTTGCCATCGTCTGAGGGTCGTGTGCTCAATTCTGGCTGCGTTTTACGTTACTCTGATTATTACTTTGGCAATAACCCTCGAGCCCTCGAAATCAAAGCTAAGGGAAAGGAGAGGGAGATGGCAGTTTTTAACCAAAGCTCAGAGTTCCAGAATTTTAAGTACTCAACACTAGTGAAAGCAACCGAGTGCTTCGATGAAGCCCATAAGCTTGGTCAAGGAGCATATGGTGAAGTATTTAAGGGCACTTTACATGATGGTAGAGAAATTGCTATAAAACGTTTGTTCATAACCGGAAAAACCCAAACTCAAGAGGTGTACAATGAAATTCATATCATTAGAAAAGCTCAACATAAGAACTTGGTTCGCTTCCTTGGCTGCTGCTTCACCAACATCAGCTGTTTCCTTATCTACGAATTCCTAGCAAACAGAAGCCTCGACCTTATATTATTTGGTAAGCCAGCTTGA

Protein Analysis

325

Amino Acids

36.95

Weight (kDa)

8.29

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 18 - 81 3e-10 Salt stress response/antifungal
Stress-antifung PF01657 96 - 191 2.2e-12 Salt stress response/antifungal
Pkinase PF00069 236 - 318 7.7e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 238 - 320 1.1e-13 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 227
AciI CCGC 1 cut(s) 68
AclI AACGTT 1 cut(s) 790
AcsI RAATTY 4 cut(s) 314, 664, 837, 926
AfaI GTAC 2 cut(s) 674, 830
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 4 cut(s) 12, 280, 307, 343
AhlI ACTAGT 1 cut(s) 682
AjiI CACGTC 1 cut(s) 147
AluBI AGCT 8 cut(s) 246, 399, 611, 651, 721, 857, 909, 974
AluI AGCT 8 cut(s) 246, 399, 611, 651, 721, 857, 909, 974
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 1 cut(s) 251
Ama87I CYCGRG 1 cut(s) 591
ApeKI GCWGC 5 cut(s) 288, 491, 549, 888, 891
ApoI RAATTY 4 cut(s) 314, 664, 837, 926
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
Asp700I GAANNNNTTC 1 cut(s) 338
AsuHPI GGTGA 3 cut(s) 89, 752, 889
AvaI CYCGRG 1 cut(s) 591
BaeGI GKGCMC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 598
BarI GAAGNNNNNNTAC 2 cut(s) 457, 489
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 5 cut(s) 300, 503, 536, 875, 878
BccI CCATC 6 cut(s) 24, 209, 368, 526, 625, 761
BcoDI GTCTC 1 cut(s) 251
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 3 cut(s) 455, 683, 932
BglII AGATCT 1 cut(s) 121
BisI GCNGC 5 cut(s) 289, 492, 550, 889, 892
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 5 cut(s) 290, 493, 551, 890, 893
BmcAI AGTACT 1 cut(s) 674
BmeT110I CYCGRG 1 cut(s) 591
BmgBI CACGTC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 466
Bpu10I CCTNAGC 1 cut(s) 612
Bpu1102I GCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 804
BsaJI CCNNGG 2 cut(s) 171, 883
BsaWI WCCGGW 1 cut(s) 803
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 90
Bse3DI GCAATG 1 cut(s) 283
BseDI CCNNGG 2 cut(s) 171, 883
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 5 cut(s) 17, 122, 354, 516, 666
BseRI GAGGAG 1 cut(s) 463
BseSI GKGCMC 1 cut(s) 758
BseXI GCAGC 5 cut(s) 300, 503, 536, 875, 878
BsiHKAI GWGCWC 1 cut(s) 540
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 2 cut(s) 91, 804
BslFI GGGAC 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 3 cut(s) 540, 598, 758
Bsp1407I TGTACA 1 cut(s) 828
Bsp143I GATC 1 cut(s) 121
Bsp1720I GCTNAGC 1 cut(s) 363
BspACI CCGC 1 cut(s) 68
BspCNI CTCAG 5 cut(s) 18, 121, 355, 517, 665
BsrDI GCAATG 1 cut(s) 283
BsrFI RCCGGY 1 cut(s) 90
BsrGI TGTACA 1 cut(s) 828
BssAI RCCGGY 1 cut(s) 90
BssECI CCNNGG 2 cut(s) 171, 883
BssMI GATC 1 cut(s) 121
BssT1I CCWWGG 2 cut(s) 171, 883
Bst4CI ACNGT 3 cut(s) 424, 461, 500
Bst6I CTCTTC 1 cut(s) 66
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 2 cut(s) 515, 972
BstDEI CTNAG 6 cut(s) 26, 108, 363, 525, 612, 652
BstENI CCTNNNNNAGG 1 cut(s) 192
BstKTI GATC 1 cut(s) 124
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 1 cut(s) 121
BstMWI GCNNNNNNNGC 2 cut(s) 500, 885
BstSLI GKGCMC 1 cut(s) 758
BstV1I GCAGC 5 cut(s) 300, 503, 536, 875, 878
BstX2I RGATCY 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 722
BstYI RGATCY 1 cut(s) 121
BtrI CACGTC 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 116, 429
Cac8I GCNNGC 2 cut(s) 515, 972
Cfr10I RCCGGY 1 cut(s) 90
Csp6I GTAC 2 cut(s) 673, 829
CviAII CATG 5 cut(s) 115, 167, 477, 510, 764
CviQI GTAC 2 cut(s) 673, 829
DdeI CTNAG 6 cut(s) 26, 108, 363, 525, 612, 652
DpnI GATC 1 cut(s) 123
DpnII GATC 1 cut(s) 121
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 171, 883
Eco24I GRGCYC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 591
EcoNI CCTNNNNNAGG 1 cut(s) 192
EcoRI GAATTC 1 cut(s) 926
EcoT14I CCWWGG 2 cut(s) 171, 883
EcoT38I GRGCYC 1 cut(s) 598
ErhI CCWWGG 2 cut(s) 171, 883
FaeI CATG 5 cut(s) 118, 170, 480, 513, 767
FaqI GGGAC 1 cut(s) 76
FatI CATG 5 cut(s) 114, 166, 476, 509, 763
FauNDI CATATG 1 cut(s) 736
Fnu4HI GCNGC 5 cut(s) 289, 492, 550, 889, 892
FriOI GRGCYC 1 cut(s) 598
Fsp4HI GCNGC 5 cut(s) 289, 492, 550, 889, 892
FspBI CTAG 3 cut(s) 455, 683, 932
GluI GCNGC 5 cut(s) 289, 492, 550, 889, 892
GsuI CTGGAG 1 cut(s) 466
HapII CCGG 2 cut(s) 91, 804
Hin1II CATG 5 cut(s) 118, 170, 480, 513, 767
HindIII AAGCTT 1 cut(s) 719
HinfI GANTC 1 cut(s) 451
HpaII CCGG 2 cut(s) 91, 804
HphI GGTGA 3 cut(s) 89, 752, 889
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 4 cut(s) 312, 526, 568, 655
Hpy188III TCNNGA 4 cut(s) 86, 455, 661, 821
Hpy8I GTNNAC 1 cut(s) 829
HpyAV CCTTC 2 cut(s) 18, 194
HpyCH4III ACNGT 3 cut(s) 424, 461, 500
HpyCH4IV ACGT 3 cut(s) 146, 559, 790
HpyCH4V TGCA 5 cut(s) 114, 288, 383, 491, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 500, 885
HpyF3I CTNAG 6 cut(s) 26, 108, 363, 525, 612, 652
HpySE526I ACGT 3 cut(s) 146, 559, 790
Hsp92II CATG 5 cut(s) 118, 170, 480, 513, 767
Kzo9I GATC 1 cut(s) 121
LmnI GCTCC 2 cut(s) 51, 731
LpnPI CCDG 9 cut(s) 99, 104, 162, 180, 342, 430, 531, 674, 817
Lsp1109I GCAGC 5 cut(s) 300, 503, 536, 875, 878
MaeI CTAG 3 cut(s) 455, 683, 932
MaeII ACGT 3 cut(s) 146, 559, 790
MaeIII GTNAC 3 cut(s) 3, 50, 560
MalI GATC 1 cut(s) 123
MboI GATC 1 cut(s) 121
MboII GAAGA 2 cut(s) 83, 465
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 3 cut(s) 540, 598, 758
MluCI AATT 9 cut(s) 238, 314, 439, 504, 541, 664, 777, 837, 926
MlyI GAGTC 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 5 cut(s) 318, 411, 642, 669, 750
MslI CAYNNNNRTG 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 909
MspI CCGG 2 cut(s) 91, 804
MwoI GCNNNNNNNGC 2 cut(s) 500, 885
NdeI CATATG 1 cut(s) 736
NdeII GATC 1 cut(s) 121
NlaIII CATG 5 cut(s) 118, 170, 480, 513, 767
NmeAIII GCCGAG 1 cut(s) 325
PaeR7I CTCGAG 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 338
PkrI GCNGC 5 cut(s) 290, 493, 551, 890, 893
PleI GAGTC 1 cut(s) 459
PpsI GAGTC 1 cut(s) 459
PsiI TTATAA 1 cut(s) 227
Psp1406I AACGTT 1 cut(s) 790
PspXI VCTCGAGB 1 cut(s) 591
PsrI GAACNNNNNNTAC 2 cut(s) 454, 486
PsuI RGATCY 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 909
RsaI GTAC 2 cut(s) 674, 830
RsaNI GTAC 2 cut(s) 673, 829
RseI CAYNNNNRTG 1 cut(s) 372
SaqAI TTAA 5 cut(s) 318, 411, 642, 669, 750
SatI GCNGC 5 cut(s) 289, 492, 550, 889, 892
Sau3AI GATC 1 cut(s) 121
ScaI AGTACT 1 cut(s) 674
SchI GAGTC 1 cut(s) 460
SduI GDGCHC 3 cut(s) 540, 598, 758
Sfr274I CTCGAG 1 cut(s) 591
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 1 cut(s) 372
SmlI CTYRAG 2 cut(s) 591, 819
SmoI CTYRAG 2 cut(s) 591, 819
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 9 cut(s) 238, 314, 439, 504, 541, 664, 777, 837, 926
SsiI CCGC 1 cut(s) 68
SspMI CTAG 3 cut(s) 455, 683, 932
StyI CCWWGG 2 cut(s) 171, 883
TaaI ACNGT 3 cut(s) 424, 461, 500
TaiI ACGT 3 cut(s) 149, 562, 793
TaqI TCGA 4 cut(s) 592, 600, 705, 948
TaqII GACCGA 1 cut(s) 118
TasI AATT 9 cut(s) 238, 314, 439, 504, 541, 664, 777, 837, 926
TatI WGTACW 2 cut(s) 672, 828
Tru1I TTAA 5 cut(s) 318, 411, 642, 669, 750
Tru9I TTAA 5 cut(s) 318, 411, 642, 669, 750
TscAI CASTG 2 cut(s) 116, 429
TseI GCWGC 5 cut(s) 288, 491, 549, 888, 891
TspDTI ATGAA 7 cut(s) 150, 465, 498, 723, 787, 830, 849
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 2 cut(s) 116, 429
XagI CCTNNNNNAGG 1 cut(s) 192
XapI RAATTY 4 cut(s) 314, 664, 837, 926
XbaI TCTAGA 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 338
XspI CTAG 3 cut(s) 455, 683, 932
ZrmI AGTACT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.