RchiOBHm_Chr2g0173461

Pentatricopeptide repeat-containing protein At4g35850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003017
Physical Location & Seq
Forward (+)
86454449 .. 86459104
4656 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54068

Sequence Viewer

Length: 1356 bp
ATGACACTCCTCCGATCAATTTCCGGTGCTTCTCACCGGTCACTGTTTCTTCGTGCCATCGGCCGGCGACACCTGGCTTCTACTCCGGCTCCGGCTCCGGCTCAGGCGGAGGAATACAAGAGGAGAAACTACGCCAACAATGTCACGGAGTACAACGGCGTCGTTAACTCCATCACTGCGCAGAGAAGGTTCTCTTTTCTTCTGAGAGACGTCTACGACGACATGTCGCTCGACGGAGTCCAGCCGGACCGAGACACCTTCCACTCGCTCATTTTGGGGTCCATGAAAGGGGCTCGCTTGCAGGACGCGTTCTACTTCACTGAGCAGATGAAATCCATGGGCTTGGTTCCTGATGTCAATTCGTACAACATGTTGATTTCGGTGTGTGGGAAGTGCAAGAACTCGGAGCAGGCGATTCAGTTATTGGAGGAAATGAAGAAGTATAAAGTAAAGCCGATGGTGCAAACCTACATGTGTCTCCTCAATGCATGTGCAGCTGCTGGCCGTCTAGATCAAGTGTATGCAATTGTCCGCGACATGACTGCTGCTGGTCTTGGGTTAAACAAGTTTTGCTATGCGGGACTTATAACTGCACTGAGGAACAAGACACCCTTACCAGATGATTATGCTGCCAAAATTATAGAGTTTGTGGAGCGGTCAAAAGAGTGGTCATCGGTAGAAGCTTCAAGTGAAACAGCTCAAAATGTAATGACGGGGGTAACAGAGGAGGAGCTGTATAACATGCCAACAGCTGAGTATATTAATAGGCGTGGATTTTTGAATAGGCCACTAACCGTGTATCATGTTGCATTTCATGCATGTGCGGACCTTCGAAATGTTGAGGTGATGGAAGCTCTTTTGGACATGCTAAAAAAGGATGGGAAATTTCCTGATACCTATATTGTGATTCAAACTATGAGATGCTATTTGAACTGTGGGGATATTGATCGTGGTTGGCAAACGGTTGATGAATATCTGAAGTCAGGAAGGCCTCCTGTACCAGAACTCTATACGACACTTGCAGAGGGTGCCATGATTGGATATACTCCTACGGGAATGCAAATTGCCCAAAAGGCATTGGAAGATATGAATTCCAGGAACTTTTACTTGCTCCCAAGACAAGGGACTGATCTTCTCCTTGCAGCTGCCGGTGAAAAGACTGGAGGTTATACTACTGCAAACTATATATGGGACTTGATGCAAGCTCGTAAAGTAACTCCTCAACTTCCTGCAGTAGAAGCATATTACAATGGCTTGAAAGAACGCGAGATTCCTGCTGATGATCCACGACTAGTACTGGTTAAGCGTACTTATGAAAACCTTTCTTCGAGATTCAGAGGACGACGACCAAATTAA

Protein Analysis

451

Amino Acids

50.91

Weight (kDa)

8.45

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 70 - 181 1.7e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 71 - 131 3.4e-09 Pentatricopeptide repeat domain
PPR_3 PF13812 108 - 164 1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 113 - 146 1.6e-06 PPR repeat
PPR_2 PF13041 117 - 165 6.8e-12 PPR repeat family
PPR PF01535 121 - 148 8.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AatII GACGTC 1 cut(s) 213
Acc16I TGCGCA 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 1028
AccBSI CCGCTC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 213
AccII CGCG 3 cut(s) 308, 534, 1266
AciI CCGC 5 cut(s) 107, 532, 578, 655, 824
AclWI GGATC 1 cut(s) 1277
AcoI YGGCCR 2 cut(s) 61, 502
AcsI RAATTY 2 cut(s) 884, 1090
AcuI CTGAAG 1 cut(s) 998
AcyI GRCGYC 2 cut(s) 159, 210
AfaI GTAC 5 cut(s) 152, 365, 999, 1296, 1309
AfiI CCNNNNNNNGG 3 cut(s) 63, 288, 1121
AflIII ACRYGT 4 cut(s) 222, 306, 369, 471
AgeI ACCGGT 1 cut(s) 36
AgsI TTSAA 5 cut(s) 687, 781, 911, 931, 1258
AhdI GACNNNNNGTC 1 cut(s) 223
AhlI ACTAGT 1 cut(s) 1291
AjnI CCWGG 2 cut(s) 72, 1094
AluBI AGCT 8 cut(s) 497, 683, 698, 733, 752, 854, 1145, 1205
AluI AGCT 8 cut(s) 497, 683, 698, 733, 752, 854, 1145, 1205
Alw26I GTCTC 3 cut(s) 201, 246, 482
AlwI GGATC 1 cut(s) 1277
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 4 cut(s) 61, 502, 785, 989
ApeKI GCWGC 6 cut(s) 494, 497, 545, 629, 1142, 1145
ApoI RAATTY 2 cut(s) 884, 1090
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AseI ATTAAT 1 cut(s) 762
AsiGI ACCGGT 1 cut(s) 36
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 3 cut(s) 247, 279, 826
AsuHPI GGTGA 3 cut(s) 26, 856, 1163
AsuII TTCGAA 1 cut(s) 832
AvaII GGWCC 3 cut(s) 247, 279, 826
BanI GGYRCC 1 cut(s) 1028
BanII GRGCYC 1 cut(s) 295
BbvI GCAGC 6 cut(s) 484, 506, 532, 616, 1132, 1154
BccI CCATC 5 cut(s) 65, 179, 451, 841, 872
BceAI ACGGC 2 cut(s) 172, 489
BcgI CGANNNNNNTGC 4 cut(s) 524, 558, 1256, 1290
BciT130I CCWGG 2 cut(s) 74, 1096
BcoDI GTCTC 3 cut(s) 201, 246, 482
BcuI ACTAGT 1 cut(s) 1291
BfaI CTAG 2 cut(s) 509, 1292
BfmI CTRYAG 1 cut(s) 1230
BglI GCCNNNNNGGC 1 cut(s) 1073
BisI GCNGC 6 cut(s) 495, 498, 546, 630, 1143, 1146
BlsI GCNGC 6 cut(s) 496, 499, 547, 631, 1144, 1147
BmcAI AGTACT 1 cut(s) 1296
Bme1390I CCNGG 2 cut(s) 74, 1096
Bme18I GGWCC 3 cut(s) 247, 279, 826
BmeRI GACNNNNNGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 247, 279, 826
BmiI GGNNCC 5 cut(s) 90, 96, 280, 348, 1030
BmrFI CCNGG 2 cut(s) 74, 1096
BmsI GCATC 2 cut(s) 911, 1188
BpmI CTGGAG 1 cut(s) 1182
Bpu10I CCTNAGC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 832
BsaBI GATNNNNATC 2 cut(s) 945, 972
BsaHI GRCGYC 2 cut(s) 159, 210
BsaJI CCNNGG 1 cut(s) 336
BsaWI WCCGGW 2 cut(s) 23, 36
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 719, 749
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 288, 1121
Bse118I RCCGGY 3 cut(s) 36, 63, 1148
Bse1I ACTGG 2 cut(s) 1165, 1302
Bse8I GATNNNNATC 2 cut(s) 945, 972
BseBI CCWGG 2 cut(s) 74, 1096
BseDI CCNNGG 1 cut(s) 336
BseGI GGATG 1 cut(s) 883
BseJI GATNNNNATC 2 cut(s) 945, 972
BseLI CCNNNNNNNGG 3 cut(s) 63, 288, 1121
BseMII CTCAG 5 cut(s) 116, 194, 312, 587, 744
BseNI ACTGG 2 cut(s) 1165, 1302
BseRI GAGGAG 5 cut(s) 136, 470, 740, 743, 1209
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 6 cut(s) 484, 506, 532, 616, 1132, 1154
BsgI GTGCAG 2 cut(s) 513, 576
Bsh1236I CGCG 3 cut(s) 308, 534, 1266
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 63, 504, 787, 991
BshNI GGYRCC 1 cut(s) 1028
BshTI ACCGGT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 245, 1149
BslFI GGGAC 3 cut(s) 594, 1138, 1205
BslI CCNNNNNNNGG 3 cut(s) 63, 288, 1121
BsmAI GTCTC 3 cut(s) 201, 246, 482
BsmBI CGTCTC 1 cut(s) 201
BsmFI GGGAC 3 cut(s) 594, 1138, 1205
BsmI GAATGC 1 cut(s) 1062
BsnI GGCC 4 cut(s) 63, 504, 787, 991
Bsp119I TTCGAA 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 5 cut(s) 14, 511, 946, 1129, 1282
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 5 cut(s) 107, 532, 578, 655, 824
BspANI GGCC 4 cut(s) 63, 504, 787, 991
BspCNI CTCAG 5 cut(s) 115, 195, 313, 588, 745
BspFNI CGCG 3 cut(s) 308, 534, 1266
BspLI GGNNCC 5 cut(s) 90, 96, 280, 348, 1030
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 1 cut(s) 1277
BspT104I TTCGAA 1 cut(s) 832
BspT107I GGYRCC 1 cut(s) 1028
BsrBI CCGCTC 1 cut(s) 655
BsrFI RCCGGY 3 cut(s) 36, 63, 1148
BsrI ACTGG 2 cut(s) 1165, 1302
BssAI RCCGGY 3 cut(s) 36, 63, 1148
BssECI CCNNGG 1 cut(s) 336
BssMI GATC 5 cut(s) 14, 511, 946, 1129, 1282
BssNI GRCGYC 2 cut(s) 159, 210
BssT1I CCWWGG 1 cut(s) 336
Bst2UI CCWGG 2 cut(s) 74, 1096
Bst4CI ACNGT 4 cut(s) 45, 796, 935, 964
BstACI GRCGYC 2 cut(s) 159, 210
BstAPI GCANNNNNTGC 2 cut(s) 815, 1028
BstBI TTCGAA 1 cut(s) 832
BstC8I GCNNGC 6 cut(s) 65, 295, 299, 411, 502, 1203
BstDEI CTNAG 5 cut(s) 102, 203, 321, 596, 753
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 883
BstFNI CGCG 3 cut(s) 308, 534, 1266
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 5 cut(s) 17, 514, 949, 1132, 1285
BstMAI GTCTC 3 cut(s) 201, 246, 482
BstMBI GATC 5 cut(s) 14, 511, 946, 1129, 1282
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 6 cut(s) 460, 494, 815, 1028, 1073, 1238
BstNI CCWGG 2 cut(s) 74, 1096
BstNSI RCATGY 7 cut(s) 226, 373, 475, 492, 745, 822, 868
BstSCI CCNGG 2 cut(s) 72, 1094
BstSFI CTRYAG 1 cut(s) 1230
BstUI CGCG 3 cut(s) 308, 534, 1266
BstV1I GCAGC 6 cut(s) 484, 506, 532, 616, 1132, 1154
BstXI CCANNNNNNTGG 1 cut(s) 343
BstZI CGGCCG 1 cut(s) 61
BsuRI GGCC 4 cut(s) 63, 504, 787, 991
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 883
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 4 cut(s) 41, 174, 318, 593
Cac8I GCNNGC 6 cut(s) 65, 295, 299, 411, 502, 1203
CaiI CAGNNNCTG 1 cut(s) 500
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 3 cut(s) 36, 63, 1148
Cfr13I GGNCC 3 cut(s) 247, 279, 826
CpoI CGGWCCG 1 cut(s) 247
CseI GACGC 2 cut(s) 148, 314
Csp6I GTAC 5 cut(s) 151, 364, 998, 1295, 1308
CspAI ACCGGT 1 cut(s) 36
CspI CGGWCCG 1 cut(s) 247
CviQI GTAC 5 cut(s) 151, 364, 998, 1295, 1308
DdeI CTNAG 5 cut(s) 102, 203, 321, 596, 753
DpnI GATC 5 cut(s) 16, 513, 948, 1131, 1284
DpnII GATC 5 cut(s) 14, 511, 946, 1129, 1282
DriI GACNNNNNGTC 1 cut(s) 223
EaeI YGGCCR 2 cut(s) 61, 502
EagI CGGCCG 1 cut(s) 61
Eam1105I GACNNNNNGTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 122
EclXI CGGCCG 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 336
Eco147I AGGCCT 1 cut(s) 991
Eco24I GRGCYC 1 cut(s) 295
Eco47I GGWCC 3 cut(s) 247, 279, 826
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 998
EcoRI GAATTC 1 cut(s) 1090
EcoRII CCWGG 2 cut(s) 72, 1094
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 2 cut(s) 490, 820
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 1 cut(s) 201
FalI AAGNNNNNCTT 4 cut(s) 178, 210, 596, 628
FaqI GGGAC 3 cut(s) 594, 1138, 1205
FauI CCCGC 1 cut(s) 571
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 6 cut(s) 495, 498, 546, 630, 1143, 1146
FokI GGATG 1 cut(s) 890
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 6 cut(s) 495, 498, 546, 630, 1143, 1146
FspBI CTAG 2 cut(s) 509, 1292
FspI TGCGCA 1 cut(s) 180
GlaI GCGC 1 cut(s) 180
GluI GCNGC 6 cut(s) 495, 498, 546, 630, 1143, 1146
GsuI CTGGAG 1 cut(s) 1182
HaeIII GGCC 4 cut(s) 63, 504, 787, 991
HapII CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 245, 1149
HgaI GACGC 2 cut(s) 148, 314
HhaI GCGC 1 cut(s) 181
Hin1I GRCGYC 2 cut(s) 159, 210
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 5 cut(s) 237, 415, 907, 1270, 1332
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 245, 1149
HphI GGTGA 3 cut(s) 26, 856, 1163
Hpy166II GTNNAC 2 cut(s) 166, 214
Hpy188I TCNGA 5 cut(s) 14, 204, 406, 978, 1337
Hpy188III TCNNGA 5 cut(s) 350, 509, 890, 984, 1329
Hpy8I GTNNAC 2 cut(s) 166, 214
Hpy99I CGWCG 4 cut(s) 164, 221, 236, 1347
HpyAV CCTTC 4 cut(s) 180, 268, 839, 981
HpyCH4III ACNGT 4 cut(s) 45, 796, 935, 964
HpyCH4IV ACGT 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 6 cut(s) 460, 494, 815, 1028, 1073, 1238
HpyF3I CTNAG 5 cut(s) 102, 203, 321, 596, 753
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 2 cut(s) 159, 210
HspAI GCGC 1 cut(s) 179
KroI GCCGGC 1 cut(s) 63
KroNI GCCGGC 1 cut(s) 65
KspAI GTTAAC 1 cut(s) 166
Kzo9I GATC 5 cut(s) 14, 511, 946, 1129, 1282
LmnI GCTCC 6 cut(s) 94, 100, 406, 652, 730, 1116
Lsp1109I GCAGC 6 cut(s) 484, 506, 532, 616, 1132, 1154
LweI GCATC 2 cut(s) 911, 1188
MaeI CTAG 2 cut(s) 509, 1292
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 4 cut(s) 39, 142, 718, 1213
MalI GATC 5 cut(s) 16, 513, 948, 1131, 1284
MbiI CCGCTC 1 cut(s) 655
MboI GATC 5 cut(s) 14, 511, 946, 1129, 1282
MboII GAAGA 6 cut(s) 41, 191, 448, 1094, 1124, 1317
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 8 cut(s) 18, 358, 525, 636, 884, 1062, 1090, 1351
MluI ACGCGT 1 cut(s) 306
MlyI GAGTC 1 cut(s) 246
Mph1103I ATGCAT 2 cut(s) 490, 820
MroNI GCCGGC 1 cut(s) 63
MseI TTAA 5 cut(s) 165, 560, 762, 1302, 1354
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 3 cut(s) 497, 752, 1145
MspI CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 245, 1149
MspR9I CCNGG 2 cut(s) 74, 1096
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 1062
MvaI CCWGG 2 cut(s) 74, 1096
MvnI CGCG 3 cut(s) 308, 534, 1266
MwoI GCNNNNNNNGC 6 cut(s) 460, 494, 815, 1028, 1073, 1238
NaeI GCCGGC 1 cut(s) 65
NcoI CCATGG 1 cut(s) 336
NdeII GATC 5 cut(s) 14, 511, 946, 1129, 1282
NgoMIV GCCGGC 1 cut(s) 63
NlaIV GGNNCC 5 cut(s) 90, 96, 280, 348, 1030
NmuCI GTSAC 2 cut(s) 39, 142
NsbI TGCGCA 1 cut(s) 180
NsiI ATGCAT 2 cut(s) 490, 820
NspI RCATGY 7 cut(s) 226, 373, 475, 492, 745, 822, 868
NspV TTCGAA 1 cut(s) 832
PceI AGGCCT 1 cut(s) 991
PciI ACATGT 3 cut(s) 222, 369, 471
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 410
PctI GAATGC 1 cut(s) 1062
PdiI GCCGGC 1 cut(s) 65
PfeI GAWTC 4 cut(s) 415, 907, 1270, 1332
PflFI GACNNNGTC 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 1094
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 6 cut(s) 496, 499, 547, 631, 1144, 1147
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PscI ACATGT 3 cut(s) 222, 369, 471
PshBI ATTAAT 1 cut(s) 762
PsiI TTATAA 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 72, 1094
PspGI CCWGG 2 cut(s) 72, 1094
PspN4I GGNNCC 5 cut(s) 90, 96, 280, 348, 1030
PspPI GGNCC 3 cut(s) 247, 279, 826
PstI CTGCAG 1 cut(s) 1234
PstNI CAGNNNCTG 1 cut(s) 500
PsyI GACNNNGTC 1 cut(s) 236
PvuII CAGCTG 3 cut(s) 497, 752, 1145
RsaI GTAC 5 cut(s) 152, 365, 999, 1296, 1309
RsaNI GTAC 5 cut(s) 151, 364, 998, 1295, 1308
RseI CAYNNNNRTG 1 cut(s) 819
Rsr2I CGGWCCG 1 cut(s) 247
RsrII CGGWCCG 1 cut(s) 247
SaqAI TTAA 5 cut(s) 165, 560, 762, 1302, 1354
SatI GCNGC 6 cut(s) 495, 498, 546, 630, 1143, 1146
Sau3AI GATC 5 cut(s) 14, 511, 946, 1129, 1282
Sau96I GGNCC 3 cut(s) 247, 279, 826
ScaI AGTACT 1 cut(s) 1296
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 2 cut(s) 74, 1096
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 2 cut(s) 911, 1188
SfcI CTRYAG 1 cut(s) 1230
SfuI TTCGAA 1 cut(s) 832
SinI GGWCC 3 cut(s) 247, 279, 826
SmiMI CAYNNNNRTG 1 cut(s) 819
SpeI ACTAGT 1 cut(s) 1291
Sse9I AATT 8 cut(s) 18, 358, 525, 636, 884, 1062, 1090, 1351
SseBI AGGCCT 1 cut(s) 991
SsiI CCGC 5 cut(s) 107, 532, 578, 655, 824
SspMI CTAG 2 cut(s) 509, 1292
StuI AGGCCT 1 cut(s) 991
StyD4I CCNGG 2 cut(s) 72, 1094
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 4 cut(s) 45, 796, 935, 964
TaiI ACGT 1 cut(s) 213
TaqI TCGA 3 cut(s) 231, 832, 1328
TaqII GACCGA 1 cut(s) 264
TasI AATT 8 cut(s) 18, 358, 525, 636, 884, 1062, 1090, 1351
TatI WGTACW 2 cut(s) 150, 1294
TfiI GAWTC 4 cut(s) 415, 907, 1270, 1332
Tru1I TTAA 5 cut(s) 165, 560, 762, 1302, 1354
Tru9I TTAA 5 cut(s) 165, 560, 762, 1302, 1354
TscAI CASTG 4 cut(s) 48, 181, 325, 600
TseFI GTSAC 2 cut(s) 39, 142
TseI GCWGC 6 cut(s) 494, 497, 545, 629, 1142, 1145
Tsp45I GTSAC 2 cut(s) 39, 142
TspDTI ATGAA 7 cut(s) 299, 344, 449, 803, 984, 1103, 1329
TspGWI ACGGA 2 cut(s) 161, 249
TspRI CASTG 4 cut(s) 48, 181, 325, 600
Tth111I GACNNNGTC 1 cut(s) 236
VpaK11BI GGWCC 3 cut(s) 247, 279, 826
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 2 cut(s) 884, 1090
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 7 cut(s) 226, 373, 475, 492, 745, 822, 868
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 509, 1292
ZraI GACGTC 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 1296
Zsp2I ATGCAT 2 cut(s) 490, 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.