Rorug02G0568900

Pentatricopeptide repeat-containing protein At4g35850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003017
Physical Location & Seq
Forward (+)
69441714 .. 69442601
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0568900.1

Sequence Viewer

Length: 888 bp
ATGAGATCATCTCCTCCTTCCCCTACAGAAATTTCAATCAGTGGCCAACACATATCTGTATCTCAGAAAATCTCTATACGCAGCTTTCATGAGCCTTCATCCTGTCTAACAAGAAATTCGTCCCGAGCAACAAAAAAAGCTTGTATACAGCTGTCACCATGGAACCGATTCTCATTCCTCTCTACCTCTCCTTGTTTCTCCACATCCTTACTCTCCTACAGGCCAGTCAGTCACCGCCTATATACACTCCGGTGGATGATATCACCCTTGACTGTGGCAATTCGGGGCAACACTCCAATAACTATGACTCTAGAATTTGGAGTGGAGACATCAACTCAACGTTTTCCCCCATACAGGATGGTGAAAGTAACGCGACATACCAATTCAGAAAAGCACCTCCATCATTGTCAGAATCCGCCAGCCAAGTCCCTTACTCCACTGCCCGCCTTTCTTACTCAGAAATCAGAATTCACTTACAGAATTCCTCTTTCTCCCGGGAAAAAGTTTGTCCGCTTGTATTTCAACCCAGCTTCATACCCGGACTTCCACAACTCCAACTCCCTCTTCTCTGTCACAGCTAGCGACTACATCCTCCTCAAAGACTTCAGAGCTTACATCACTGCCGATGATGTTTATAGCGAGACGCCACTAATGAGAGAATTCTGCGTCAACATAGAGACAGAACATAGCTTGAACATCACATTCACACCCAGCAAAGATGCATATGCTTTCATCAACGGGATTGAAATCGTGTCGATGCCCACCAATCTTTACACCACTTCAGTGCGTTCCATCGGCAGCGATAACCCCTATTCAGTTGAAAGCAGAAAAGCTCTGGACATGGTGTACCAGATCAACATAGGTGGAAAGCCAAATCAACATTACTGA

Protein Analysis

295

Amino Acids

33.5

Weight (kDa)

9.47

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 153 - 273 2.5e-07 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 145
AccII CGCG 1 cut(s) 373
AciI CCGC 4 cut(s) 235, 416, 444, 511
AclI AACGTT 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 43
AcsI RAATTY 6 cut(s) 30, 115, 314, 467, 480, 659
AcuI CTGAAG 2 cut(s) 589, 765
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 1 cut(s) 354
AgsI TTSAA 5 cut(s) 36, 523, 694, 746, 821
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AleI CACNNNNGTG 2 cut(s) 250, 782
AluBI AGCT 8 cut(s) 84, 140, 151, 530, 578, 611, 690, 833
AluI AGCT 8 cut(s) 84, 140, 151, 530, 578, 611, 690, 833
Alw26I GTCTC 3 cut(s) 320, 635, 671
Ama87I CYCGRG 2 cut(s) 123, 494
AoxI GGCC 2 cut(s) 43, 221
ApeKI GCWGC 2 cut(s) 81, 798
ApoI RAATTY 6 cut(s) 30, 115, 314, 467, 480, 659
Asp700I GAANNNNTTC 1 cut(s) 167
AsuC2I CCSGG 3 cut(s) 495, 496, 539
AsuHPI GGTGA 4 cut(s) 147, 224, 255, 373
AsuNHI GCTAGC 1 cut(s) 578
AvaI CYCGRG 2 cut(s) 123, 494
BalI TGGCCA 1 cut(s) 45
BbvI GCAGC 2 cut(s) 93, 810
BccI CCATC 3 cut(s) 352, 408, 800
BcnI CCSGG 3 cut(s) 495, 496, 539
BcoDI GTCTC 3 cut(s) 320, 635, 671
BfaI CTAG 2 cut(s) 311, 579
BfmI CTRYAG 2 cut(s) 24, 217
BisI GCNGC 2 cut(s) 82, 799
BlsI GCNGC 2 cut(s) 83, 800
Bme1390I CCNGG 3 cut(s) 495, 496, 539
BmeT110I CYCGRG 2 cut(s) 123, 494
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 3 cut(s) 495, 496, 539
BmsI GCATC 2 cut(s) 709, 747
BmtI GCTAGC 1 cut(s) 582
BplI GAGNNNNNCTC 1 cut(s) 27
BpuMI CCSGG 3 cut(s) 495, 496, 539
BsaBI GATNNNNATC 1 cut(s) 746
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 2 cut(s) 158, 494
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 1 cut(s) 224
Bse8I GATNNNNATC 1 cut(s) 746
BseDI CCNNGG 2 cut(s) 158, 494
BseGI GGATG 5 cut(s) 98, 203, 261, 363, 588
BseJI GATNNNNATC 1 cut(s) 746
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMII CTCAG 2 cut(s) 77, 470
BseNI ACTGG 1 cut(s) 224
BseRI GAGGAG 1 cut(s) 584
BseXI GCAGC 2 cut(s) 93, 810
BseYI CCCAGC 2 cut(s) 526, 710
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 2 cut(s) 45, 223
BsiHKCI CYCGRG 2 cut(s) 123, 494
BsiSI CCGG 3 cut(s) 250, 495, 539
BslFI GGGAC 2 cut(s) 106, 412
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 3 cut(s) 320, 635, 671
BsmBI CGTCTC 1 cut(s) 635
BsmFI GGGAC 2 cut(s) 106, 412
BsnI GGCC 2 cut(s) 45, 223
BsoBI CYCGRG 2 cut(s) 123, 494
Bsp143I GATC 2 cut(s) 5, 852
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 4 cut(s) 235, 416, 444, 511
BspANI GGCC 2 cut(s) 45, 223
BspCNI CTCAG 2 cut(s) 76, 469
BspFNI CGCG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 164
BspOI GCTAGC 1 cut(s) 582
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 158, 494
BssMI GATC 2 cut(s) 5, 852
BssNAI GTATAC 1 cut(s) 146
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 1 cut(s) 158
Bst1107I GTATAC 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 569
BstACI GRCGYC 1 cut(s) 644
BstC8I GCNNGC 3 cut(s) 420, 444, 580
BstDEI CTNAG 2 cut(s) 63, 456
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 5 cut(s) 98, 203, 261, 363, 588
BstFNI CGCG 1 cut(s) 373
BstKTI GATC 2 cut(s) 8, 855
BstMAI GTCTC 3 cut(s) 320, 635, 671
BstMBI GATC 2 cut(s) 5, 852
BstSCI CCNGG 3 cut(s) 493, 494, 537
BstSFI CTRYAG 2 cut(s) 24, 217
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 93, 810
BstZ17I GTATAC 1 cut(s) 146
BsuRI GGCC 2 cut(s) 45, 223
BtgI CCRYGG 1 cut(s) 158
BtsCI GGATG 5 cut(s) 98, 203, 261, 363, 588
BtsI GCAGTG 2 cut(s) 437, 618
BtsIMutI CAGTG 4 cut(s) 46, 437, 618, 789
Cac8I GCNNGC 3 cut(s) 420, 444, 580
CciI TCATGA 1 cut(s) 88
Cfr9I CCCGGG 1 cut(s) 494
CseI GACGC 2 cut(s) 652, 655
Csp6I GTAC 1 cut(s) 847
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviAII CATG 3 cut(s) 89, 159, 841
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 2 cut(s) 63, 456
DpnI GATC 2 cut(s) 7, 854
DpnII GATC 2 cut(s) 5, 852
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
EciI GGCGGA 1 cut(s) 405
Eco130I CCWWGG 1 cut(s) 158
Eco32I GATATC 1 cut(s) 261
Eco57I CTGAAG 2 cut(s) 589, 765
Eco88I CYCGRG 2 cut(s) 123, 494
EcoRI GAATTC 3 cut(s) 467, 480, 659
EcoRV GATATC 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 158
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 3 cut(s) 92, 162, 844
FaqI GGGAC 2 cut(s) 106, 412
FatI CATG 3 cut(s) 88, 158, 840
FauI CCCGC 1 cut(s) 451
FauNDI CATATG 1 cut(s) 724
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 2 cut(s) 82, 799
FokI GGATG 5 cut(s) 85, 190, 268, 370, 575
Fsp4HI GCNGC 2 cut(s) 82, 799
FspBI CTAG 2 cut(s) 311, 579
GluI GCNGC 2 cut(s) 82, 799
GsaI CCCAGC 2 cut(s) 530, 714
HaeIII GGCC 2 cut(s) 45, 223
HapII CCGG 3 cut(s) 250, 495, 539
HgaI GACGC 2 cut(s) 652, 655
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 3 cut(s) 92, 162, 844
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 1 cut(s) 138
HinfI GANTC 3 cut(s) 168, 307, 412
HpaII CCGG 3 cut(s) 250, 495, 539
HphI GGTGA 4 cut(s) 147, 224, 255, 373
Hpy166II GTNNAC 3 cut(s) 146, 670, 847
Hpy188I TCNGA 6 cut(s) 66, 388, 411, 459, 466, 608
Hpy188III TCNNGA 4 cut(s) 89, 123, 311, 836
Hpy8I GTNNAC 3 cut(s) 146, 670, 847
HpyAV CCTTC 2 cut(s) 27, 105
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 1 cut(s) 722
HpyF3I CTNAG 2 cut(s) 63, 456
HpySE526I ACGT 1 cut(s) 340
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 3 cut(s) 92, 162, 844
Kzo9I GATC 2 cut(s) 5, 852
Lsp1109I GCAGC 2 cut(s) 93, 810
LweI GCATC 2 cut(s) 709, 747
MaeI CTAG 2 cut(s) 311, 579
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 4 cut(s) 153, 230, 367, 571
MalI GATC 2 cut(s) 7, 854
MboI GATC 2 cut(s) 5, 852
MboII GAAGA 1 cut(s) 556
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 8 cut(s) 30, 115, 279, 314, 382, 467, 480, 659
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 1 cut(s) 579
MnlI CCTC 8 cut(s) 24, 188, 196, 407, 495, 572, 602, 605
Mox20I TGGCCA 1 cut(s) 45
Mph1103I ATGCAT 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 167
MscI TGGCCA 1 cut(s) 45
MslI CAYNNNNRTG 2 cut(s) 250, 782
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 3 cut(s) 250, 495, 539
MspR9I CCNGG 3 cut(s) 495, 496, 539
MvnI CGCG 1 cut(s) 373
NciI CCSGG 3 cut(s) 495, 496, 539
NcoI CCATGG 1 cut(s) 158
NdeI CATATG 1 cut(s) 724
NdeII GATC 2 cut(s) 5, 852
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 3 cut(s) 92, 162, 844
NlaIV GGNNCC 1 cut(s) 164
NmuCI GTSAC 3 cut(s) 153, 230, 571
NsiI ATGCAT 1 cut(s) 724
OliI CACNNNNGTG 2 cut(s) 250, 782
PagI TCATGA 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 168, 412
PkrI GCNGC 2 cut(s) 83, 800
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Psp1406I AACGTT 1 cut(s) 340
PspFI CCCAGC 2 cut(s) 526, 710
PspN4I GGNNCC 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 151
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 2 cut(s) 250, 782
SatI GCNGC 2 cut(s) 82, 799
Sau3AI GATC 2 cut(s) 5, 852
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 3 cut(s) 495, 496, 539
SfaNI GCATC 2 cut(s) 709, 747
SfcI CTRYAG 2 cut(s) 24, 217
SmaI CCCGGG 1 cut(s) 496
SmiMI CAYNNNNRTG 2 cut(s) 250, 782
Sse9I AATT 8 cut(s) 30, 115, 279, 314, 382, 467, 480, 659
SsiI CCGC 4 cut(s) 235, 416, 444, 511
SspMI CTAG 2 cut(s) 311, 579
StyD4I CCNGG 3 cut(s) 493, 494, 537
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 343
TaqI TCGA 1 cut(s) 755
TasI AATT 8 cut(s) 30, 115, 279, 314, 382, 467, 480, 659
TfiI GAWTC 2 cut(s) 168, 412
TscAI CASTG 4 cut(s) 46, 444, 625, 789
TseFI GTSAC 3 cut(s) 153, 230, 571
TseI GCWGC 2 cut(s) 81, 798
Tsp45I GTSAC 3 cut(s) 153, 230, 571
TspDTI ATGAA 4 cut(s) 77, 87, 522, 721
TspMI CCCGGG 1 cut(s) 494
TspRI CASTG 4 cut(s) 46, 444, 625, 789
XapI RAATTY 6 cut(s) 30, 115, 314, 467, 480, 659
XbaI TCTAGA 1 cut(s) 310
XmaI CCCGGG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 2 cut(s) 311, 579
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.