RLG00000022192

Pentatricopeptide repeat-containing protein At4g35850

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003017
Physical Location & Seq
Forward (+)
81630158 .. 81634214
4057 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022192

Sequence Viewer

Length: 1362 bp
ATGACACTCCTCCGATCAATTTCCGGTGCTTCTCACCGGTCACCACTCCTCCGAGCCCTCGGCCGGAGACACCTGGCTTCTGCTCCGGCTCCGGCTCCGGCTATGGCTCAGAAGGAGGAATACAAGAGGAGAAACTACGCCAACAATGTCACGGAGTACAACGGCGTCGTTAACTCCATCACTGCGCAGAGAAGGTTCTCTTTTCTTCTGAGGGACGTTTACGACGACATGTCGCTCGACGGAGTCCAGCCGGACCGAGACACCTTCCACTCGCTCATTTTGGGGTCCGTGAAAGGGGCTCGGTTGCAGGACGCGTTCTACTTCACTGAGCAGATGAAATCCATGGGCTTGGTTCCTGATGTCAATTCGTACAACATGTTGATTTCGGTGTGTGGGAAGTGCAAGAACTCGGAGCAGGCGATTCAGATTTTGGAAGAAATGAAGAAGTATAAGGTAAAGCCGATGGTGCAAACCTACATGTGTCTCCTCAATGCATGTGCAGCTGCTGGCCGTCTAGATCAAGTGTATGCAATTGTCCGCGACATGACTGCTGCTGGTCTTGGGTTAAACAAGTTTTGCTATGCGGGACTTATAACTGCACTGAGGAACAAGACACCCTTACCAGATGATTATGCTGCCAAAATTATAGAGTTTGTGGAGCGGTCAAAAGAGTGGTCATCGGTGGAAGCTTCAAGTGAAACAGCTCAAAATGTAATGACGGGGGTAACAGAAGAGGAGTTGTATAACATGCCAACAGCTGAGTATATTAATAGGCGTGGATTTTTGAATAGGCCACTAACTGTGTATCATGTTGCATTTCATGCTTGTGCGGACCTTCGAAATGTTGAGGTGATGGAAGCTCTTTTGGACATGCTAAAAAAGGACGGGAAATTTCCTGATACCTATATTGTGATTCAAACTATGAGATGCTATTTGAACTGTGGGGATATTGATCGTGGTTGGCAAACGCTTGATGAATATCTGAAGTCAGGAAGGCCTCCCGTACCAGAACTCTATACAACACTTGCAGAGGGCGCCATGATTGGATATACTCCTAAGGGAATGCAAATTGCCCAAAAGGCATTGGAAGATATGAATTCCAGGAACTTTCACTTGCTCCCAAGACAAGGGACTGATCTTCTCCTTGTAGCTGCCGGTGAAAAGACTGGAGGTTATACTACTGCAAACTATATATGGGACTTGATGCAAGCTCGTAAAGTAACTCCTCAACTTCCTGCAGTAGAAGCATATTACAATGGCTTGAAAGAACGCGAGATTCCTGCTGATGATCCACGCCTAGTACTGGTTAAGCGTACTTATGAAAACCTTTCTTCGAGATTCAGAGGACGACGACCAAATTAA

Protein Analysis

454

Amino Acids

51.11

Weight (kDa)

8.71

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 71 - 183 5.9e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 73 - 133 7.2e-09 Pentatricopeptide repeat domain
PPR_3 PF13812 110 - 166 1.2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 115 - 148 7.9e-06 PPR repeat
PPR_2 PF13041 119 - 167 2.4e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
Acc16I TGCGCA 1 cut(s) 186
AccB1I GGYRCC 1 cut(s) 1034
AccBSI CCGCTC 1 cut(s) 661
AccII CGCG 3 cut(s) 314, 540, 1272
AciI CCGC 4 cut(s) 538, 584, 661, 830
AclWI GGATC 1 cut(s) 1283
AcoI YGGCCR 2 cut(s) 61, 508
AcsI RAATTY 2 cut(s) 890, 1096
AcuI CTGAAG 1 cut(s) 1004
AcyI GRCGYC 2 cut(s) 165, 1035
AfaI GTAC 5 cut(s) 158, 371, 1005, 1302, 1315
AfiI CCNNNNNNNGG 4 cut(s) 63, 294, 1127, 1303
AflIII ACRYGT 4 cut(s) 228, 312, 375, 477
AgeI ACCGGT 1 cut(s) 36
AgsI TTSAA 5 cut(s) 693, 787, 917, 937, 1264
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 72, 1100
AluBI AGCT 7 cut(s) 503, 689, 704, 758, 860, 1151, 1211
AluI AGCT 7 cut(s) 503, 689, 704, 758, 860, 1151, 1211
Alw26I GTCTC 3 cut(s) 61, 252, 488
AlwI GGATC 1 cut(s) 1283
AlwNI CAGNNNCTG 1 cut(s) 506
AoxI GGCC 4 cut(s) 61, 508, 791, 995
ApeKI GCWGC 5 cut(s) 500, 503, 551, 635, 1151
ApoI RAATTY 2 cut(s) 890, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
AseI ATTAAT 1 cut(s) 768
AsiGI ACCGGT 1 cut(s) 36
AspLEI GCGC 2 cut(s) 187, 1037
AspS9I GGNCC 3 cut(s) 253, 285, 832
AsuHPI GGTGA 4 cut(s) 26, 33, 862, 1169
AsuII TTCGAA 1 cut(s) 838
AvaII GGWCC 3 cut(s) 253, 285, 832
AxyI CCTNAGG 1 cut(s) 1056
BanI GGYRCC 1 cut(s) 1034
BanII GRGCYC 2 cut(s) 58, 301
BarI GAAGNNNNNNTAC 2 cut(s) 104, 136
BbvI GCAGC 5 cut(s) 490, 512, 538, 622, 1138
BccI CCATC 3 cut(s) 185, 457, 847
BceAI ACGGC 2 cut(s) 178, 495
BcgI CGANNNNNNTGC 4 cut(s) 530, 564, 1262, 1296
BciT130I CCWGG 2 cut(s) 74, 1102
BcoDI GTCTC 3 cut(s) 61, 252, 488
BfaI CTAG 2 cut(s) 515, 1298
BfmI CTRYAG 1 cut(s) 1236
BfoI RGCGCY 1 cut(s) 1038
BglI GCCNNNNNGGC 1 cut(s) 1079
BisI GCNGC 5 cut(s) 501, 504, 552, 636, 1152
BlsI GCNGC 5 cut(s) 502, 505, 553, 637, 1153
BmcAI AGTACT 1 cut(s) 1302
Bme1390I CCNGG 2 cut(s) 74, 1102
Bme18I GGWCC 3 cut(s) 253, 285, 832
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 253, 285, 832
BmiI GGNNCC 5 cut(s) 90, 96, 286, 354, 1036
BmrFI CCNGG 2 cut(s) 74, 1102
BmsI GCATC 2 cut(s) 917, 1194
BpmI CTGGAG 1 cut(s) 1188
Bpu14I TTCGAA 1 cut(s) 838
BsaBI GATNNNNATC 2 cut(s) 951, 978
BsaHI GRCGYC 2 cut(s) 165, 1035
BsaJI CCNNGG 2 cut(s) 58, 342
BsaWI WCCGGW 2 cut(s) 23, 36
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 294, 1127, 1303
Bse118I RCCGGY 2 cut(s) 36, 1154
Bse1I ACTGG 2 cut(s) 1171, 1308
Bse21I CCTNAGG 1 cut(s) 1056
Bse8I GATNNNNATC 2 cut(s) 951, 978
BseBI CCWGG 2 cut(s) 74, 1102
BseDI CCNNGG 2 cut(s) 58, 342
BseJI GATNNNNATC 2 cut(s) 951, 978
BseLI CCNNNNNNNGG 4 cut(s) 63, 294, 1127, 1303
BseMII CTCAG 5 cut(s) 122, 200, 318, 593, 750
BseNI ACTGG 2 cut(s) 1171, 1308
BseRI GAGGAG 5 cut(s) 38, 142, 476, 749, 1215
BseX3I CGGCCG 1 cut(s) 61
BseXI GCAGC 5 cut(s) 490, 512, 538, 622, 1138
BsgI GTGCAG 2 cut(s) 519, 582
Bsh1236I CGCG 3 cut(s) 314, 540, 1272
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 63, 510, 793, 997
BshNI GGYRCC 1 cut(s) 1034
BshTI ACCGGT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 251, 1155
BslFI GGGAC 4 cut(s) 227, 600, 1144, 1211
BslI CCNNNNNNNGG 4 cut(s) 63, 294, 1127, 1303
BsmAI GTCTC 3 cut(s) 61, 252, 488
BsmFI GGGAC 4 cut(s) 227, 600, 1144, 1211
BsmI GAATGC 1 cut(s) 1068
BsnI GGCC 4 cut(s) 63, 510, 793, 997
Bsp119I TTCGAA 1 cut(s) 838
Bsp1286I GDGCHC 2 cut(s) 58, 301
Bsp143I GATC 5 cut(s) 14, 517, 952, 1135, 1288
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 4 cut(s) 538, 584, 661, 830
BspANI GGCC 4 cut(s) 63, 510, 793, 997
BspCNI CTCAG 5 cut(s) 121, 201, 319, 594, 751
BspFNI CGCG 3 cut(s) 314, 540, 1272
BspLI GGNNCC 5 cut(s) 90, 96, 286, 354, 1036
BspMAI CTGCAG 1 cut(s) 1240
BspPI GGATC 1 cut(s) 1283
BspT104I TTCGAA 1 cut(s) 838
BspT107I GGYRCC 1 cut(s) 1034
BsrBI CCGCTC 1 cut(s) 661
BsrFI RCCGGY 2 cut(s) 36, 1154
BsrI ACTGG 2 cut(s) 1171, 1308
BssAI RCCGGY 2 cut(s) 36, 1154
BssECI CCNNGG 2 cut(s) 58, 342
BssMI GATC 5 cut(s) 14, 517, 952, 1135, 1288
BssNI GRCGYC 2 cut(s) 165, 1035
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 2 cut(s) 74, 1102
Bst4CI ACNGT 2 cut(s) 802, 941
Bst6I CTCTTC 1 cut(s) 726
BstACI GRCGYC 2 cut(s) 165, 1035
BstAPI GCANNNNNTGC 1 cut(s) 821
BstBI TTCGAA 1 cut(s) 838
BstC8I GCNNGC 3 cut(s) 417, 508, 1209
BstDEI CTNAG 6 cut(s) 108, 209, 327, 602, 759, 1056
BstDSI CCRYGG 1 cut(s) 342
BstEII GGTNACC 1 cut(s) 39
BstFNI CGCG 3 cut(s) 314, 540, 1272
BstH2I RGCGCY 1 cut(s) 1038
BstHHI GCGC 2 cut(s) 187, 1037
BstKTI GATC 5 cut(s) 17, 520, 955, 1138, 1291
BstMAI GTCTC 3 cut(s) 61, 252, 488
BstMBI GATC 5 cut(s) 14, 517, 952, 1135, 1288
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 6 cut(s) 466, 500, 821, 1034, 1079, 1244
BstNI CCWGG 2 cut(s) 74, 1102
BstNSI RCATGY 6 cut(s) 232, 379, 481, 498, 751, 874
BstPI GGTNACC 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 72, 1100
BstSFI CTRYAG 1 cut(s) 1236
BstUI CGCG 3 cut(s) 314, 540, 1272
BstV1I GCAGC 5 cut(s) 490, 512, 538, 622, 1138
BstXI CCANNNNNNTGG 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 61
Bsu36I CCTNAGG 1 cut(s) 1056
BsuRI GGCC 4 cut(s) 63, 510, 793, 997
BtgI CCRYGG 1 cut(s) 342
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 3 cut(s) 180, 324, 599
Cac8I GCNNGC 3 cut(s) 417, 508, 1209
CaiI CAGNNNCTG 1 cut(s) 506
CfoI GCGC 2 cut(s) 187, 1037
Cfr10I RCCGGY 2 cut(s) 36, 1154
Cfr13I GGNCC 3 cut(s) 253, 285, 832
CpoI CGGWCCG 1 cut(s) 253
CseI GACGC 2 cut(s) 154, 320
Csp6I GTAC 5 cut(s) 157, 370, 1004, 1301, 1314
CspAI ACCGGT 1 cut(s) 36
CspI CGGWCCG 1 cut(s) 253
CviQI GTAC 5 cut(s) 157, 370, 1004, 1301, 1314
DdeI CTNAG 6 cut(s) 108, 209, 327, 602, 759, 1056
DinI GGCGCC 1 cut(s) 1036
DpnI GATC 5 cut(s) 16, 519, 954, 1137, 1290
DpnII GATC 5 cut(s) 14, 517, 952, 1135, 1288
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 2 cut(s) 61, 508
EagI CGGCCG 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 726
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 726
EclXI CGGCCG 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 342
Eco147I AGGCCT 1 cut(s) 997
Eco24I GRGCYC 2 cut(s) 58, 301
Eco47I GGWCC 3 cut(s) 253, 285, 832
Eco52I CGGCCG 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 1004
Eco81I CCTNAGG 1 cut(s) 1056
Eco91I GGTNACC 1 cut(s) 39
EcoO65I GGTNACC 1 cut(s) 39
EcoRI GAATTC 1 cut(s) 1096
EcoRII CCWGG 2 cut(s) 72, 1100
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 496
EcoT38I GRGCYC 2 cut(s) 58, 301
EgeI GGCGCC 1 cut(s) 1036
EheI GGCGCC 1 cut(s) 1036
ErhI CCWWGG 1 cut(s) 342
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 602, 634
FaqI GGGAC 4 cut(s) 227, 600, 1144, 1211
FauI CCCGC 1 cut(s) 577
Fnu4HI GCNGC 5 cut(s) 501, 504, 552, 636, 1152
FriOI GRGCYC 2 cut(s) 58, 301
Fsp4HI GCNGC 5 cut(s) 501, 504, 552, 636, 1152
FspBI CTAG 2 cut(s) 515, 1298
FspI TGCGCA 1 cut(s) 186
GlaI GCGC 2 cut(s) 186, 1036
GluI GCNGC 5 cut(s) 501, 504, 552, 636, 1152
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 1038
HaeIII GGCC 4 cut(s) 63, 510, 793, 997
HapII CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 251, 1155
HgaI GACGC 2 cut(s) 154, 320
HhaI GCGC 2 cut(s) 187, 1037
Hin1I GRCGYC 2 cut(s) 165, 1035
Hin6I GCGC 2 cut(s) 185, 1035
HinP1I GCGC 2 cut(s) 185, 1035
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 687
HinfI GANTC 5 cut(s) 243, 421, 913, 1276, 1338
HpaI GTTAAC 1 cut(s) 172
HpaII CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 251, 1155
HphI GGTGA 4 cut(s) 26, 33, 862, 1169
Hpy166II GTNNAC 2 cut(s) 172, 220
Hpy188I TCNGA 8 cut(s) 14, 53, 111, 210, 412, 426, 984, 1343
Hpy188III TCNNGA 5 cut(s) 356, 515, 896, 990, 1335
Hpy8I GTNNAC 2 cut(s) 172, 220
Hpy99I CGWCG 4 cut(s) 170, 227, 242, 1353
HpyAV CCTTC 5 cut(s) 106, 186, 274, 845, 987
HpyCH4III ACNGT 2 cut(s) 802, 941
HpyCH4IV ACGT 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 6 cut(s) 466, 500, 821, 1034, 1079, 1244
HpyF3I CTNAG 6 cut(s) 108, 209, 327, 602, 759, 1056
HpySE526I ACGT 1 cut(s) 216
Hsp92I GRCGYC 2 cut(s) 165, 1035
HspAI GCGC 2 cut(s) 185, 1035
KasI GGCGCC 1 cut(s) 1034
KspAI GTTAAC 1 cut(s) 172
Kzo9I GATC 5 cut(s) 14, 517, 952, 1135, 1288
LmnI GCTCC 6 cut(s) 88, 94, 100, 412, 658, 1122
Lsp1109I GCAGC 5 cut(s) 490, 512, 538, 622, 1138
LweI GCATC 2 cut(s) 917, 1194
MaeI CTAG 2 cut(s) 515, 1298
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 4 cut(s) 39, 148, 724, 1219
MalI GATC 5 cut(s) 16, 519, 954, 1137, 1290
MbiI CCGCTC 1 cut(s) 661
MboI GATC 5 cut(s) 14, 517, 952, 1135, 1288
MboII GAAGA 7 cut(s) 197, 446, 454, 743, 1100, 1130, 1323
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 58, 301
MluCI AATT 8 cut(s) 18, 364, 531, 642, 890, 1068, 1096, 1357
MluI ACGCGT 1 cut(s) 312
Mly113I GGCGCC 1 cut(s) 1035
MlyI GAGTC 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 496
MseI TTAA 5 cut(s) 171, 566, 768, 1308, 1360
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 2 cut(s) 503, 758
MspI CCGG 8 cut(s) 24, 37, 64, 86, 92, 98, 251, 1155
MspR9I CCNGG 2 cut(s) 74, 1102
MunI CAATTG 1 cut(s) 531
Mva1269I GAATGC 1 cut(s) 1068
MvaI CCWGG 2 cut(s) 74, 1102
MvnI CGCG 3 cut(s) 314, 540, 1272
MwoI GCNNNNNNNGC 6 cut(s) 466, 500, 821, 1034, 1079, 1244
NarI GGCGCC 1 cut(s) 1035
NcoI CCATGG 1 cut(s) 342
NdeII GATC 5 cut(s) 14, 517, 952, 1135, 1288
NlaIV GGNNCC 5 cut(s) 90, 96, 286, 354, 1036
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 2 cut(s) 39, 148
NsbI TGCGCA 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 496
NspI RCATGY 6 cut(s) 232, 379, 481, 498, 751, 874
NspV TTCGAA 1 cut(s) 838
PceI AGGCCT 1 cut(s) 997
PciI ACATGT 3 cut(s) 228, 375, 477
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 416
PctI GAATGC 1 cut(s) 1068
PfeI GAWTC 4 cut(s) 421, 913, 1276, 1338
PflFI GACNNNGTC 1 cut(s) 242
PfoI TCCNGGA 1 cut(s) 1100
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 5 cut(s) 502, 505, 553, 637, 1153
PleI GAGTC 1 cut(s) 251
PluTI GGCGCC 1 cut(s) 1038
PpsI GAGTC 1 cut(s) 251
PscI ACATGT 3 cut(s) 228, 375, 477
PshBI ATTAAT 1 cut(s) 768
PsiI TTATAA 1 cut(s) 593
Psp6I CCWGG 2 cut(s) 72, 1100
PspEI GGTNACC 1 cut(s) 39
PspGI CCWGG 2 cut(s) 72, 1100
PspN4I GGNNCC 5 cut(s) 90, 96, 286, 354, 1036
PspPI GGNCC 3 cut(s) 253, 285, 832
PstI CTGCAG 1 cut(s) 1240
PstNI CAGNNNCTG 1 cut(s) 506
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 2 cut(s) 503, 758
RsaI GTAC 5 cut(s) 158, 371, 1005, 1302, 1315
RsaNI GTAC 5 cut(s) 157, 370, 1004, 1301, 1314
RseI CAYNNNNRTG 1 cut(s) 825
Rsr2I CGGWCCG 1 cut(s) 253
RsrII CGGWCCG 1 cut(s) 253
SaqAI TTAA 5 cut(s) 171, 566, 768, 1308, 1360
SatI GCNGC 5 cut(s) 501, 504, 552, 636, 1152
Sau3AI GATC 5 cut(s) 14, 517, 952, 1135, 1288
Sau96I GGNCC 3 cut(s) 253, 285, 832
ScaI AGTACT 1 cut(s) 1302
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 2 cut(s) 74, 1102
SduI GDGCHC 2 cut(s) 58, 301
SfaNI GCATC 2 cut(s) 917, 1194
SfcI CTRYAG 1 cut(s) 1236
SfoI GGCGCC 1 cut(s) 1036
SfuI TTCGAA 1 cut(s) 838
SinI GGWCC 3 cut(s) 253, 285, 832
SmiMI CAYNNNNRTG 1 cut(s) 825
Sse9I AATT 8 cut(s) 18, 364, 531, 642, 890, 1068, 1096, 1357
SseBI AGGCCT 1 cut(s) 997
SsiI CCGC 4 cut(s) 538, 584, 661, 830
SspDI GGCGCC 1 cut(s) 1034
SspMI CTAG 2 cut(s) 515, 1298
StuI AGGCCT 1 cut(s) 997
StyD4I CCNGG 2 cut(s) 72, 1100
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 802, 941
TaiI ACGT 1 cut(s) 219
TaqI TCGA 3 cut(s) 237, 838, 1334
TaqII GACCGA 1 cut(s) 270
TasI AATT 8 cut(s) 18, 364, 531, 642, 890, 1068, 1096, 1357
TatI WGTACW 2 cut(s) 156, 1300
TfiI GAWTC 4 cut(s) 421, 913, 1276, 1338
Tru1I TTAA 5 cut(s) 171, 566, 768, 1308, 1360
Tru9I TTAA 5 cut(s) 171, 566, 768, 1308, 1360
TscAI CASTG 3 cut(s) 187, 331, 606
TseFI GTSAC 2 cut(s) 39, 148
TseI GCWGC 5 cut(s) 500, 503, 551, 635, 1151
Tsp45I GTSAC 2 cut(s) 39, 148
TspDTI ATGAA 6 cut(s) 350, 455, 809, 990, 1109, 1335
TspGWI ACGGA 3 cut(s) 167, 255, 277
TspRI CASTG 3 cut(s) 187, 331, 606
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 3 cut(s) 253, 285, 832
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 2 cut(s) 890, 1096
XbaI TCTAGA 1 cut(s) 514
XceI RCATGY 6 cut(s) 232, 379, 481, 498, 751, 874
XspI CTAG 2 cut(s) 515, 1298
ZrmI AGTACT 1 cut(s) 1302
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.