RchiOBHm_Chr5g0036691

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004879
Physical Location & Seq
Reverse (-)
30889319 .. 30889763
445 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31550

Sequence Viewer

Length: 390 bp
ATGCTCCACTACGCCTACGGCGCCGTCAAGAAGTCGATTGAGGCCTACGTCACTCGCGCCGGCGGGCACGTCATTGAGGTACCCTTGCCGTTTCCTTTGAATTCCAACGATGAGATTGTAACCGAGTTTAAGAAGGCTTTGGAGAAGGGGAAGGCTAATGGTAGGAGGGTTAGGTTAGCTGTGATTGATCATATTACTTCAATGCCGTGTGTAGTTATACCTGTTGAGGAGTTGGTTAAGATTTGTAGGGAGGAGGGAGTTGATCAGGTTTTCATCGATGCGGCACACAGCATTGGGTGCACTGAGGTTGATATGAAGGAGATTGGGGCTGATTATTATACTAGAGTCGCCCAAGGGTGTGGAATTGCACCACCCGGTGGTTTCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

129

Amino Acids

14.03

Weight (kDa)

5.78

Isoelectric Point (pI)

45.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 55 - 117 1.2e-06 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 79
AccB1I GGYRCC 2 cut(s) 20, 79
AccB7I CCANNNNNTGG 1 cut(s) 377
AccII CGCG 1 cut(s) 57
AciI CCGC 2 cut(s) 63, 281
AcsI RAATTY 1 cut(s) 100
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 1 cut(s) 81
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 100, 201
AjiI CACGTC 1 cut(s) 70
AluBI AGCT 1 cut(s) 179
AluI AGCT 1 cut(s) 179
Alw21I GWGCWC 1 cut(s) 302
Alw44I GTGCAC 1 cut(s) 298
AoxI GGCC 1 cut(s) 42
ApaLI GTGCAC 1 cut(s) 298
ApoI RAATTY 1 cut(s) 100
Asp718I GGTACC 1 cut(s) 79
AspLEI GCGC 2 cut(s) 23, 59
AsuC2I CCSGG 1 cut(s) 375
BaeGI GKGCMC 2 cut(s) 69, 302
BanI GGYRCC 2 cut(s) 20, 79
Bbv12I GWGCWC 1 cut(s) 302
BceAI ACGGC 4 cut(s) 8, 34, 73, 190
BclI TGATCA 2 cut(s) 187, 262
BcnI CCSGG 1 cut(s) 375
BfaI CTAG 1 cut(s) 342
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 1 cut(s) 375
BmgBI CACGTC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 22, 81
BmrFI CCNGG 1 cut(s) 375
BmsI GCATC 1 cut(s) 268
BpuMI CCSGG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 352
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse118I RCCGGY 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 352
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 1 cut(s) 294
BseRI GAGGAG 2 cut(s) 242, 266
BseSI GKGCMC 2 cut(s) 69, 302
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 44
BshNI GGYRCC 2 cut(s) 20, 79
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 2 cut(s) 60, 375
BslI CCNNNNNNNGG 1 cut(s) 377
BsnI GGCC 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 69, 302
Bsp143I GATC 2 cut(s) 187, 262
BspACI CCGC 2 cut(s) 63, 281
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 1 cut(s) 295
BspDI ATCGAT 1 cut(s) 276
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 22, 81
BspT107I GGYRCC 2 cut(s) 20, 79
BsrFI RCCGGY 1 cut(s) 59
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 2 cut(s) 187, 262
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 352
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 61, 65
BstDEI CTNAG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 57
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 59
BstKTI GATC 2 cut(s) 190, 265
BstMBI GATC 2 cut(s) 187, 262
BstMWI GCNNNNNNNGC 2 cut(s) 20, 297
BstSCI CCNGG 1 cut(s) 373
BstSLI GKGCMC 2 cut(s) 69, 302
BstUI CGCG 1 cut(s) 57
BstXI CCANNNNNNTGG 1 cut(s) 359
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 1 cut(s) 44
BsuTUI ATCGAT 1 cut(s) 276
BtrI CACGTC 1 cut(s) 70
BtsIMutI CAGTG 1 cut(s) 300
Cac8I GCNNGC 2 cut(s) 61, 65
CfoI GCGC 2 cut(s) 23, 59
Cfr10I RCCGGY 1 cut(s) 59
ClaI ATCGAT 1 cut(s) 276
Csp6I GTAC 1 cut(s) 80
CviAII CATG 1 cut(s) 387
CviJI RGCY 5 cut(s) 44, 137, 155, 179, 329
CviKI_1 RGCY 5 cut(s) 44, 137, 155, 179, 329
CviQI GTAC 1 cut(s) 80
DdeI CTNAG 1 cut(s) 303
DinI GGCGCC 1 cut(s) 22
DpnI GATC 2 cut(s) 189, 264
DpnII GATC 2 cut(s) 187, 262
DraIII CACNNNGTG 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 352
Eco147I AGGCCT 1 cut(s) 44
EcoRI GAATTC 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 352
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 1 cut(s) 390
FaiI YATR 5 cut(s) 192, 218, 314, 339, 388
FatI CATG 1 cut(s) 386
FauI CCCGC 1 cut(s) 56
FbaI TGATCA 2 cut(s) 187, 262
Fnu4HI GCNGC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 282
FspBI CTAG 1 cut(s) 342
GlaI GCGC 2 cut(s) 22, 58
GluI GCNGC 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 60, 375
HhaI GCGC 2 cut(s) 23, 59
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 1 cut(s) 390
Hin6I GCGC 2 cut(s) 21, 57
HinP1I GCGC 2 cut(s) 21, 57
HinfI GANTC 1 cut(s) 345
HpaII CCGG 2 cut(s) 60, 375
Hpy166II GTNNAC 1 cut(s) 300
Hpy188III TCNNGA 1 cut(s) 28
Hpy8I GTNNAC 1 cut(s) 300
HpyAV CCTTC 4 cut(s) 127, 139, 145, 310
HpyCH4IV ACGT 2 cut(s) 48, 69
HpyCH4V TGCA 2 cut(s) 300, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 20, 297
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 2 cut(s) 48, 69
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 1 cut(s) 390
HspAI GCGC 2 cut(s) 21, 57
KasI GGCGCC 1 cut(s) 20
KpnI GGTACC 1 cut(s) 83
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Ksp22I TGATCA 2 cut(s) 187, 262
Kzo9I GATC 2 cut(s) 187, 262
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 3 cut(s) 73, 234, 251
LweI GCATC 1 cut(s) 268
MaeI CTAG 1 cut(s) 342
MaeII ACGT 2 cut(s) 48, 69
MaeIII GTNAC 2 cut(s) 49, 118
MalI GATC 2 cut(s) 189, 264
MboI GATC 2 cut(s) 187, 262
MhlI GDGCHC 2 cut(s) 69, 302
MluCI AATT 2 cut(s) 100, 363
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 354
MmeI TCCRAC 1 cut(s) 129
MnlI CCTC 7 cut(s) 34, 70, 159, 220, 244, 247, 298
MreI CGCCGGCG 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 2 cut(s) 129, 237
MspI CCGG 2 cut(s) 60, 375
MspR9I CCNGG 1 cut(s) 375
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 20, 297
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 1 cut(s) 375
NdeII GATC 2 cut(s) 187, 262
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 1 cut(s) 390
NlaIV GGNNCC 2 cut(s) 22, 81
NmuCI GTSAC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 44
PdiI GCCGGC 1 cut(s) 61
PflMI CCANNNNNTGG 1 cut(s) 377
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 1 cut(s) 353
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 353
PspN4I GGNNCC 2 cut(s) 22, 81
RsaI GTAC 1 cut(s) 81
RsaNI GTAC 1 cut(s) 80
SaqAI TTAA 2 cut(s) 129, 237
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 2 cut(s) 187, 262
SchI GAGTC 1 cut(s) 354
ScrFI CCNGG 1 cut(s) 375
SduI GDGCHC 2 cut(s) 69, 302
SetI ASST 8 cut(s) 51, 72, 81, 176, 181, 223, 270, 309
SfaNI GCATC 1 cut(s) 268
SfoI GGCGCC 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 59
Sse9I AATT 2 cut(s) 100, 363
SseBI AGGCCT 1 cut(s) 44
SsiI CCGC 2 cut(s) 63, 281
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 1 cut(s) 342
StuI AGGCCT 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 373
StyI CCWWGG 1 cut(s) 352
TaiI ACGT 2 cut(s) 51, 72
TaqI TCGA 2 cut(s) 35, 276
TasI AATT 2 cut(s) 100, 363
TauI GCSGC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 129, 237
Tru9I TTAA 2 cut(s) 129, 237
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 2 cut(s) 262, 329
TspRI CASTG 1 cut(s) 307
Van91I CCANNNNNTGG 1 cut(s) 377
VneI GTGCAC 1 cut(s) 298
XapI RAATTY 1 cut(s) 100
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.