Rh4AG368700

Catalyzes the phosphorylation of pantothenate the first step in CoA biosynthesis. May play a role in the physiological regulation of the intracellular CoA concentration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004879
Physical Location & Seq
Forward (+)
67650301 .. 67652260
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG368700.1

Sequence Viewer

Length: 684 bp
ATGGCTCCGGTCACCGGAAGTTCGATTCACCGGTCGGGCTCTCGGCCTCAGTTGGACCTCAGCAAAGCTGCTATTCAGGGAACTTTTGAAGAGAGGGACCCTACAATTTTGTTGCCTAATCAGTCTGATCACTTGTCTCATCTGGCTTTGGACATTGGAGGATCTCTCATCAAGTTGGTGTATTTTTCTAGACACGAAGACCAATCAATTGATGACAAAAGGAAGAAAACTTTGAAGGAGAGACTGGGTATTTCCAATGGTAGTCGAAGAAGCTACCCTGTTCTTGGTGGAAGGCTTCATTTTGTGAAGTTTGAGACGACCAAGATTAATGAGTGCTTAGACTTCATTTATTCTAAACAACTTCACCGTGGAGGGATGGATGTTCGTATTTGGAATCCTGATGCCCCAACCAATGAAAATGCTATAATTAAGGGTTCCCCAAAGGCTGCAGCCTTTGAGCGTAAGAGTAATGGCGAATTGCATATGATTGATGATTCAACTCCCGAGGTTTCTCTCACTCTCCCTTACGCCAATTTCGATTTTCTTAATATCCAAAACTTGAAATACTACTTGAAAGGTTGGGGCATGTGTCATTCTGGTTTAATTGTCTTGACTTGGTCTCTACTGTATCAACATCATCTTAATTCGGCTTGTGTTACCTACAAGAAAAGCATTTCCAGGTGA

Protein Analysis

227

Amino Acids

25.61

Weight (kDa)

9.18

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fumble PF03630 47 - 125 7.9e-14 Fumble
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 169
AfiI CCNNNNNNNGG 2 cut(s) 14, 284
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 5 cut(s) 89, 235, 498, 562, 574
AjnI CCWGG 1 cut(s) 677
AluBI AGCT 2 cut(s) 68, 273
AluI AGCT 2 cut(s) 68, 273
Alw26I GTCTC 4 cut(s) 141, 235, 308, 624
AlwI GGATC 1 cut(s) 169
Ama87I CYCGRG 1 cut(s) 503
AoxI GGCC 1 cut(s) 44
ApeKI GCWGC 3 cut(s) 68, 446, 449
AseI ATTAAT 1 cut(s) 327
AsiGI ACCGGT 1 cut(s) 30
AspS9I GGNCC 2 cut(s) 55, 97
AsuHPI GGTGA 3 cut(s) 4, 20, 356
AvaI CYCGRG 1 cut(s) 503
AvaII GGWCC 2 cut(s) 55, 97
BanII GRGCYC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 55, 433, 461
BccI CCATC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 679
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 4 cut(s) 141, 235, 308, 624
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 447
BisI GCNGC 3 cut(s) 69, 447, 450
BlsI GCNGC 3 cut(s) 70, 448, 451
Bme1390I CCNGG 1 cut(s) 679
Bme18I GGWCC 2 cut(s) 55, 97
BmeT110I CYCGRG 1 cut(s) 503
BmgT120I GGNCC 2 cut(s) 55, 97
BmiI GGNNCC 4 cut(s) 6, 98, 99, 436
BmrFI CCNGG 1 cut(s) 679
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 391
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 624
BsaJI CCNNGG 2 cut(s) 367, 504
BsaWI WCCGGW 3 cut(s) 7, 14, 30
Bsc4I CCNNNNNNNGG 2 cut(s) 14, 284
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 679
BseDI CCNNGG 2 cut(s) 367, 504
BseGI GGATG 2 cut(s) 381, 385
BseLI CCNNNNNNNGG 2 cut(s) 14, 284
BseMII CTCAG 2 cut(s) 62, 73
BseNI ACTGG 1 cut(s) 249
BseXI GCAGC 3 cut(s) 55, 433, 461
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 46
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 503
BsiSI CCGG 3 cut(s) 8, 15, 31
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 14, 284
BsmAI GTCTC 4 cut(s) 141, 235, 308, 624
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 1 cut(s) 46
Bso31I GGTCTC 1 cut(s) 624
BsoBI CYCGRG 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 2 cut(s) 127, 161
BspANI GGCC 1 cut(s) 46
BspCNI CTCAG 2 cut(s) 61, 72
BspLI GGNNCC 4 cut(s) 6, 98, 99, 436
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 624
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 2 cut(s) 367, 504
BssMI GATC 2 cut(s) 127, 161
Bst2UI CCWGG 1 cut(s) 679
Bst4CI ACNGT 2 cut(s) 368, 627
Bst6I CTCTTC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 48, 59, 337
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 2 cut(s) 381, 385
BstKTI GATC 2 cut(s) 130, 164
BstMAI GTCTC 4 cut(s) 141, 235, 308, 624
BstMBI GATC 2 cut(s) 127, 161
BstMCI CGRYCG 1 cut(s) 35
BstNI CCWGG 1 cut(s) 679
BstNSI RCATGY 1 cut(s) 589
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 677
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 55, 433, 461
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
BsuRI GGCC 1 cut(s) 46
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 2 cut(s) 381, 385
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 2 cut(s) 55, 97
CspAI ACCGGT 1 cut(s) 30
CviAII CATG 1 cut(s) 586
DdeI CTNAG 3 cut(s) 48, 59, 337
DpnI GATC 2 cut(s) 129, 163
DpnII GATC 2 cut(s) 127, 161
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 41
Eco31I GGTCTC 1 cut(s) 624
Eco47I GGWCC 2 cut(s) 55, 97
Eco88I CYCGRG 1 cut(s) 503
Eco91I GGTNACC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 677
EcoT38I GRGCYC 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 1 cut(s) 589
FaiI YATR 4 cut(s) 425, 483, 485, 587
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 585
FauNDI CATATG 1 cut(s) 483
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 3 cut(s) 69, 447, 450
FokI GGATG 2 cut(s) 388, 392
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 69, 447, 450
FspBI CTAG 1 cut(s) 189
GluI GCNGC 3 cut(s) 69, 447, 450
HaeIII GGCC 1 cut(s) 46
HapII CCGG 3 cut(s) 8, 15, 31
Hin1II CATG 1 cut(s) 589
HinfI GANTC 3 cut(s) 25, 394, 494
HpaII CCGG 3 cut(s) 8, 15, 31
HphI GGTGA 3 cut(s) 4, 20, 356
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 4 cut(s) 189, 398, 503, 610
HpyAV CCTTC 2 cut(s) 229, 285
HpyCH4III ACNGT 2 cut(s) 368, 627
HpyCH4V TGCA 2 cut(s) 449, 481
HpyF3I CTNAG 3 cut(s) 48, 59, 337
Hsp92II CATG 1 cut(s) 589
KflI GGGWCCC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 2 cut(s) 127, 161
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 3 cut(s) 55, 433, 461
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 10, 655
MalI GATC 2 cut(s) 129, 163
MboI GATC 2 cut(s) 127, 161
MboII GAAGA 4 cut(s) 101, 209, 235, 279
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 7 cut(s) 105, 207, 426, 476, 532, 603, 643
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 6 cut(s) 57, 68, 87, 152, 365, 499
MseI TTAA 5 cut(s) 327, 429, 546, 602, 642
MspI CCGG 3 cut(s) 8, 15, 31
MspR9I CCNGG 1 cut(s) 679
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 679
NdeI CATATG 1 cut(s) 483
NdeII GATC 2 cut(s) 127, 161
NlaIII CATG 1 cut(s) 589
NlaIV GGNNCC 4 cut(s) 6, 98, 99, 436
NmeAIII GCCGAG 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 10
NspI RCATGY 1 cut(s) 589
PcsI WCGNNNNNNNCGW 1 cut(s) 534
PfeI GAWTC 3 cut(s) 25, 394, 494
PflFI GACNNNGTC 1 cut(s) 616
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 3 cut(s) 70, 448, 451
PpuMI RGGWCCY 1 cut(s) 97
PshBI ATTAAT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 677
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 677
PspN4I GGNNCC 4 cut(s) 6, 98, 99, 436
PspPI GGNCC 2 cut(s) 55, 97
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 1 cut(s) 161
PsyI GACNNNGTC 1 cut(s) 616
SaqAI TTAA 5 cut(s) 327, 429, 546, 602, 642
SatI GCNGC 3 cut(s) 69, 447, 450
Sau3AI GATC 2 cut(s) 127, 161
Sau96I GGNCC 2 cut(s) 55, 97
ScrFI CCNGG 1 cut(s) 679
SduI GDGCHC 1 cut(s) 41
SetI ASST 7 cut(s) 60, 70, 275, 510, 580, 662, 683
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 2 cut(s) 55, 97
Sse9I AATT 7 cut(s) 105, 207, 426, 476, 532, 603, 643
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 677
TaaI ACNGT 2 cut(s) 368, 627
TaqI TCGA 3 cut(s) 23, 265, 537
TasI AATT 7 cut(s) 105, 207, 426, 476, 532, 603, 643
TfiI GAWTC 3 cut(s) 25, 394, 494
Tru1I TTAA 5 cut(s) 327, 429, 546, 602, 642
Tru9I TTAA 5 cut(s) 327, 429, 546, 602, 642
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 68, 446, 449
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 287, 334, 429
Tth111I GACNNNGTC 1 cut(s) 616
VpaK11BI GGWCC 2 cut(s) 55, 97
VspI ATTAAT 1 cut(s) 327
XbaI TCTAGA 1 cut(s) 188
XceI RCATGY 1 cut(s) 589
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.