Rw5G015550

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004879
Physical Location & Seq
Forward (+)
19307217 .. 19308008
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G015550.1

Sequence Viewer

Length: 696 bp
ATGGCATCCTCCCCCAACCACACCTTCAATAGTGATTCAAACACCCACGCCTCCAAAAAGTCCAAACTTTTCTTCATTACCCCCTCAGAAATTGTTGCCAAATTCTCCCACCACGACGCCAACATCGCCCGGATCAACAACGACAGCTTCGGCATCTTCCATTCCCGAACCATCATCAAGTACCACATTGATAAGGTCTCCATTGTCGACAACGCCACCACCGCCGCTGCCATCATCCTCCAGCAGACCGCCTGGGGCTTCTCCAAGAGCAAATTTGAGAAGGGCGACGCTGTTGTCATGCTCCAATACTTTTACAACGCCGTTAAGAAGTCCATTGAGGCCTATATCACTCGCGCCGGCGAGTATGTTATTGAGGTACCCTTGCCGTTTCCTTTGAATTCTAATGATGAGATTGTAACCGAGTTTAAGAAAGCTTTGGAAAAAGGGAAGGCGAATGGGAGGAGAGTTAGGCTATCTGTGATTGATCATATTACTTCAATGCCTTGTGTGGTTATACCTGTTAAGGAGTTGGTTAAGATTTGTAGGGAGGAAGGGGTTGATCAGGTTTTTGTCGATGCGGCTCACATCATTGGGTGTATGGGTATGTTGGGAATTTTCTCTGTGAGAAGGTCCCACAAAATTGCCAGATCAGACATCAACTGTCCACGTCAGCACCTTCACGAAGAAAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

231

Amino Acids

25.9

Weight (kDa)

8.99

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 293
Acc65I GGTACC 1 cut(s) 376
AccB1I GGYRCC 1 cut(s) 376
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 354
AciI CCGC 4 cut(s) 222, 225, 249, 578
AclWI GGATC 1 cut(s) 140
AcsI RAATTY 5 cut(s) 101, 272, 397, 612, 688
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 2 cut(s) 182, 378
AgsI TTSAA 4 cut(s) 28, 39, 397, 498
AjiI CACGTC 1 cut(s) 668
AjnI CCWGG 1 cut(s) 251
AjuI GAANNNNNNNTTGG 4 cut(s) 8, 40, 56, 88
AluBI AGCT 2 cut(s) 147, 434
AluI AGCT 2 cut(s) 147, 434
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 1 cut(s) 140
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 5 cut(s) 101, 272, 397, 612, 688
Asp718I GGTACC 1 cut(s) 376
AspLEI GCGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 630
AsuC2I CCSGG 1 cut(s) 130
AvaII GGWCC 1 cut(s) 630
BanI GGYRCC 1 cut(s) 376
BbvI GCAGC 1 cut(s) 214
BccI CCATC 2 cut(s) 179, 239
BceAI ACGGC 2 cut(s) 305, 370
BciT130I CCWGG 1 cut(s) 253
BclI TGATCA 2 cut(s) 484, 559
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 202
BisI GCNGC 3 cut(s) 225, 228, 579
BlsI GCNGC 3 cut(s) 226, 229, 580
Bme1390I CCNGG 2 cut(s) 130, 253
Bme18I GGWCC 1 cut(s) 630
BmgBI CACGTC 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 2 cut(s) 378, 632
BmrFI CCNGG 2 cut(s) 130, 253
BmsI GCATC 3 cut(s) 14, 162, 565
BpmI CTGGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 252
Bse118I RCCGGY 1 cut(s) 356
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 2 cut(s) 5, 234
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 475
BseXI GCAGC 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 1 cut(s) 341
BshNI GGYRCC 1 cut(s) 376
BsiSI CCGG 2 cut(s) 130, 357
BslFI GGGAC 1 cut(s) 616
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 616
BsnI GGCC 1 cut(s) 341
Bso31I GGTCTC 1 cut(s) 202
Bsp143I GATC 4 cut(s) 132, 484, 559, 647
BspACI CCGC 4 cut(s) 222, 225, 249, 578
BspANI GGCC 1 cut(s) 341
BspCNI CTCAG 1 cut(s) 98
BspFNI CGCG 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 378, 632
BspPI GGATC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 376
BspTNI GGTCTC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 356
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 4 cut(s) 132, 484, 559, 647
BssNI GRCGYC 1 cut(s) 117
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 662
BstACI GRCGYC 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 1 cut(s) 85
BstF5I GGATG 2 cut(s) 5, 234
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 4 cut(s) 135, 487, 562, 650
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 4 cut(s) 132, 484, 559, 647
BstMWI GCNNNNNNNGC 2 cut(s) 125, 221
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 2 cut(s) 128, 251
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 1 cut(s) 214
BsuRI GGCC 1 cut(s) 341
BtgZI GCGATG 1 cut(s) 109
BtrI CACGTC 1 cut(s) 668
BtsCI GGATG 2 cut(s) 5, 234
Cac8I GCNNGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 630
CseI GACGC 2 cut(s) 125, 296
Csp6I GTAC 2 cut(s) 181, 377
CviAII CATG 1 cut(s) 298
CviJI RGCY 6 cut(s) 147, 258, 341, 434, 472, 581
CviKI_1 RGCY 6 cut(s) 147, 258, 341, 434, 472, 581
CviQI GTAC 2 cut(s) 181, 377
DdeI CTNAG 1 cut(s) 85
DpnI GATC 4 cut(s) 134, 486, 561, 649
DpnII GATC 4 cut(s) 132, 484, 559, 647
DrdI GACNNNNNNGTC 1 cut(s) 293
DseDI GACNNNNNNGTC 1 cut(s) 293
Eco147I AGGCCT 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 630
EcoO109I RGGNCCY 1 cut(s) 630
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 251
FaeI CATG 1 cut(s) 301
FaiI YATR 8 cut(s) 299, 345, 366, 489, 515, 599, 605, 694
FaqI GGGAC 1 cut(s) 616
FatI CATG 1 cut(s) 297
FbaI TGATCA 2 cut(s) 484, 559
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 3 cut(s) 225, 228, 579
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 225, 228, 579
GlaI GCGC 1 cut(s) 355
GluI GCNGC 3 cut(s) 225, 228, 579
GsuI CTGGAG 1 cut(s) 224
HaeIII GGCC 1 cut(s) 341
HapII CCGG 2 cut(s) 130, 357
HgaI GACGC 2 cut(s) 125, 296
HhaI GCGC 1 cut(s) 356
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 1 cut(s) 35
HpaII CCGG 2 cut(s) 130, 357
Hpy166II GTNNAC 2 cut(s) 208, 665
Hpy188I TCNGA 2 cut(s) 88, 652
Hpy188III TCNNGA 2 cut(s) 165, 680
Hpy8I GTNNAC 2 cut(s) 208, 665
Hpy99I CGWCG 2 cut(s) 119, 290
HpyAV CCTTC 6 cut(s) 34, 274, 442, 545, 621, 686
HpyCH4III ACNGT 1 cut(s) 662
HpyCH4IV ACGT 1 cut(s) 667
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 221
HpyF3I CTNAG 1 cut(s) 85
HpySE526I ACGT 1 cut(s) 667
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 354
KpnI GGTACC 1 cut(s) 380
KroI GCCGGC 1 cut(s) 356
KroNI GCCGGC 1 cut(s) 358
Ksp22I TGATCA 2 cut(s) 484, 559
Kzo9I GATC 4 cut(s) 132, 484, 559, 647
LmnI GCTCC 1 cut(s) 306
LpnPI CCDG 8 cut(s) 143, 238, 254, 265, 370, 531, 548, 658
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 3 cut(s) 14, 162, 565
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 1 cut(s) 415
MalI GATC 4 cut(s) 134, 486, 561, 649
MboI GATC 4 cut(s) 132, 484, 559, 647
MboII GAAGA 3 cut(s) 64, 148, 695
MluCI AATT 7 cut(s) 90, 101, 272, 397, 612, 639, 688
MnlI CCTC 8 cut(s) 19, 61, 94, 248, 331, 367, 453, 541
MreI CGCCGGCG 1 cut(s) 356
MroNI GCCGGC 1 cut(s) 356
MseI TTAA 4 cut(s) 324, 426, 522, 534
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 2 cut(s) 130, 357
MspR9I CCNGG 2 cut(s) 130, 253
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 2 cut(s) 125, 221
NaeI GCCGGC 1 cut(s) 358
NciI CCSGG 1 cut(s) 130
NdeII GATC 4 cut(s) 132, 484, 559, 647
NgoMIV GCCGGC 1 cut(s) 356
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 378, 632
PceI AGGCCT 1 cut(s) 341
PdiI GCCGGC 1 cut(s) 358
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 3 cut(s) 226, 229, 580
PpuMI RGGWCCY 1 cut(s) 630
Psp5II RGGWCCY 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 2 cut(s) 378, 632
PspPI GGNCC 1 cut(s) 630
PspPPI RGGWCCY 1 cut(s) 630
RsaI GTAC 2 cut(s) 182, 378
RsaNI GTAC 2 cut(s) 181, 377
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 4 cut(s) 324, 426, 522, 534
SatI GCNGC 3 cut(s) 225, 228, 579
Sau3AI GATC 4 cut(s) 132, 484, 559, 647
Sau96I GGNCC 1 cut(s) 630
ScrFI CCNGG 2 cut(s) 130, 253
SfaNI GCATC 3 cut(s) 14, 162, 565
SgrAI CRCCGGYG 1 cut(s) 356
SinI GGWCC 1 cut(s) 630
Sse9I AATT 7 cut(s) 90, 101, 272, 397, 612, 639, 688
SseBI AGGCCT 1 cut(s) 341
SsiI CCGC 4 cut(s) 222, 225, 249, 578
StuI AGGCCT 1 cut(s) 341
StyD4I CCNGG 2 cut(s) 128, 251
TaaI ACNGT 1 cut(s) 662
TaiI ACGT 1 cut(s) 670
TaqI TCGA 2 cut(s) 207, 573
TasI AATT 7 cut(s) 90, 101, 272, 397, 612, 639, 688
TauI GCSGC 2 cut(s) 227, 581
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 4 cut(s) 324, 426, 522, 534
Tru9I TTAA 4 cut(s) 324, 426, 522, 534
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 630
XapI RAATTY 5 cut(s) 101, 272, 397, 612, 688
XmiI GTMKAC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.