Rh4DG375300

Catalyzes the phosphorylation of pantothenate the first step in CoA biosynthesis. May play a role in the physiological regulation of the intracellular CoA concentration

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004879
Physical Location & Seq
Forward (+)
59289826 .. 59291915
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG375300.1

Sequence Viewer

Length: 747 bp
ATGGCTCCGGTCACCGGAAGTTCGATTCACCGGTCGGGCTCTCGGCCTCAGTTGGACCTCAGCAAAGCTGCTATTCAGGGAACTTTTGAAGAGAGGGACCCTACAATTTTGTTGCCTAATCAGTCTGATCACTTGTCTCATCTGGCTTTGGACATTGGAGGATCTCTCATCAAGTTGGTGTATTTTTCTAGACACGAAGACCAATCAATTGATGACAAAAGGAAGAAAACTTTGAAGGAGAGACTGGGTATTTCCAATGGTAGTCGAAGAAGCTACCCTGTTCTTGGTGGAAGGCTTCATTTTGTGAAGTTTGAGACGACCAAGATTAATGAGTGCTTAGACTTCATTTATTCTAAACAACTTCACCGTGGAGGGATGGATGTTCGTATTTGGAATCCTGATGCCCCAACCAATGAAAATGCTATAATTAAGGGTTCCCCAAAGGCTGCAGCCTTTGAGCGATCCTACACTTATCCTGAAGCTAGTATTGGAATATACCGGATTCTGCGATGCAGTCCACCCTCTAAGTGTAGGTCAACAACTGATCTTAGTTCAGTCTACTCAATCCAGATCCAAAACTTGAAATACTACTTGAAAGGAAAAGCATTTCCAGGTGATGCGGTCCTAGACACGAAAGAATCCCCCCTTGTGGTTTGTTTCGGGGAGATGCTAATTGATTTCGTCCCAACCACCAATGGACTTTCATTGGCCGAAGCACCTGCATTTAAAAAAGATTGCAGAAGGTAA

Protein Analysis

248

Amino Acids

27.65

Weight (kDa)

9.22

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fumble PF03630 47 - 124 1.4e-13 Fumble
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 727
Acc36I ACCTGC 1 cut(s) 727
AccI GTMKAC 1 cut(s) 558
AciI CCGC 1 cut(s) 620
AclWI GGATC 3 cut(s) 169, 456, 565
AcoI YGGCCR 1 cut(s) 708
AcuI CTGAAG 1 cut(s) 498
AfiI CCNNNNNNNGG 3 cut(s) 14, 284, 649
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 89, 235, 583, 595
AjnI CCWGG 1 cut(s) 610
AjuI GAANNNNNNNTTGG 2 cut(s) 471, 503
AluBI AGCT 3 cut(s) 68, 273, 482
AluI AGCT 3 cut(s) 68, 273, 482
Alw26I GTCTC 3 cut(s) 141, 235, 308
AlwI GGATC 3 cut(s) 169, 456, 565
AoxI GGCC 2 cut(s) 44, 708
ApeKI GCWGC 3 cut(s) 68, 446, 449
AseI ATTAAT 1 cut(s) 327
AsiGI ACCGGT 1 cut(s) 30
AspS9I GGNCC 3 cut(s) 55, 97, 622
AsuHPI GGTGA 4 cut(s) 4, 20, 356, 626
AvaII GGWCC 3 cut(s) 55, 97, 622
BanII GRGCYC 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 204
BbvCI CCTCAGC 1 cut(s) 59
BbvI GCAGC 3 cut(s) 55, 433, 461
BccI CCATC 1 cut(s) 370
BcgI CGANNNNNNTGC 2 cut(s) 701, 735
BciT130I CCWGG 1 cut(s) 612
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 3 cut(s) 141, 235, 308
BfaI CTAG 3 cut(s) 189, 483, 626
BfmI CTRYAG 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 727
BisI GCNGC 3 cut(s) 69, 447, 450
BlsI GCNGC 3 cut(s) 70, 448, 451
Bme1390I CCNGG 1 cut(s) 612
Bme18I GGWCC 3 cut(s) 55, 97, 622
BmgT120I GGNCC 3 cut(s) 55, 97, 622
BmiI GGNNCC 4 cut(s) 6, 98, 99, 436
BmrFI CCNGG 1 cut(s) 612
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 4 cut(s) 391, 500, 607, 657
BmuI ACTGGG 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 204
BplI GAGNNNNNCTC 2 cut(s) 150, 182
Bpu10I CCTNAGC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 367
BsaWI WCCGGW 4 cut(s) 7, 14, 30, 498
Bsc4I CCNNNNNNNGG 3 cut(s) 14, 284, 649
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 381, 385
BseLI CCNNNNNNNGG 3 cut(s) 14, 284, 649
BseMII CTCAG 2 cut(s) 62, 73
BseNI ACTGG 1 cut(s) 249
BseXI GCAGC 3 cut(s) 55, 433, 461
Bsh1285I CGRYCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 46, 710
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 35
BsiSI CCGG 4 cut(s) 8, 15, 31, 499
BslFI GGGAC 2 cut(s) 110, 668
BslI CCNNNNNNNGG 3 cut(s) 14, 284, 649
BsmAI GTCTC 3 cut(s) 141, 235, 308
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 110, 668
BsnI GGCC 2 cut(s) 46, 710
Bsp1286I GDGCHC 1 cut(s) 41
Bsp143I GATC 5 cut(s) 127, 161, 461, 544, 570
BspACI CCGC 1 cut(s) 620
BspANI GGCC 2 cut(s) 46, 710
BspCNI CTCAG 2 cut(s) 61, 72
BspLI GGNNCC 4 cut(s) 6, 98, 99, 436
BspMAI CTGCAG 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 3 cut(s) 169, 456, 565
BsrFI RCCGGY 1 cut(s) 30
BsrI ACTGG 1 cut(s) 249
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 5 cut(s) 127, 161, 461, 544, 570
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 48, 59, 337, 525, 548
BstDSI CCRYGG 1 cut(s) 367
BstEII GGTNACC 1 cut(s) 10
BstF5I GGATG 2 cut(s) 381, 385
BstKTI GATC 5 cut(s) 130, 164, 464, 547, 573
BstMAI GTCTC 3 cut(s) 141, 235, 308
BstMBI GATC 5 cut(s) 127, 161, 461, 544, 570
BstMCI CGRYCG 1 cut(s) 35
BstNI CCWGG 1 cut(s) 612
BstPI GGTNACC 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 610
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 55, 433, 461
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 2 cut(s) 161, 570
BstYI RGATCY 2 cut(s) 161, 570
BsuRI GGCC 2 cut(s) 46, 710
BtgI CCRYGG 1 cut(s) 367
BtgZI GCGATG 1 cut(s) 523
BtsCI GGATG 2 cut(s) 381, 385
BveI ACCTGC 1 cut(s) 727
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 3 cut(s) 55, 97, 622
CspAI ACCGGT 1 cut(s) 30
DdeI CTNAG 5 cut(s) 48, 59, 337, 525, 548
DpnI GATC 5 cut(s) 129, 163, 463, 546, 572
DpnII GATC 5 cut(s) 127, 161, 461, 544, 570
DraI TTTAAA 1 cut(s) 727
EaeI YGGCCR 1 cut(s) 708
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco24I GRGCYC 1 cut(s) 41
Eco47I GGWCC 3 cut(s) 55, 97, 622
Eco57I CTGAAG 1 cut(s) 498
Eco91I GGTNACC 1 cut(s) 10
EcoO109I RGGNCCY 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 41
Esp3I CGTCTC 1 cut(s) 308
FaiI YATR 2 cut(s) 425, 496
FaqI GGGAC 2 cut(s) 110, 668
FbaI TGATCA 1 cut(s) 127
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 3 cut(s) 69, 447, 450
FokI GGATG 2 cut(s) 388, 392
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 3 cut(s) 69, 447, 450
FspBI CTAG 3 cut(s) 189, 483, 626
GluI GCNGC 3 cut(s) 69, 447, 450
HaeIII GGCC 2 cut(s) 46, 710
HapII CCGG 4 cut(s) 8, 15, 31, 499
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HinfI GANTC 4 cut(s) 25, 394, 502, 638
HpaII CCGG 4 cut(s) 8, 15, 31, 499
HphI GGTGA 4 cut(s) 4, 20, 356, 626
Hpy166II GTNNAC 3 cut(s) 518, 537, 559
Hpy188I TCNGA 1 cut(s) 127
Hpy188III TCNNGA 4 cut(s) 189, 398, 476, 568
Hpy8I GTNNAC 3 cut(s) 518, 537, 559
HpyAV CCTTC 3 cut(s) 229, 285, 735
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4V TGCA 4 cut(s) 449, 513, 722, 738
HpyF3I CTNAG 5 cut(s) 48, 59, 337, 525, 548
KflI GGGWCCC 1 cut(s) 97
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 5 cut(s) 127, 161, 461, 544, 570
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 3 cut(s) 55, 433, 461
LweI GCATC 4 cut(s) 391, 500, 607, 657
MaeI CTAG 3 cut(s) 189, 483, 626
MaeIII GTNAC 1 cut(s) 10
MalI GATC 5 cut(s) 129, 163, 463, 546, 572
MboI GATC 5 cut(s) 127, 161, 461, 544, 570
MboII GAAGA 4 cut(s) 101, 209, 235, 279
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 2 cut(s) 161, 570
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 4 cut(s) 105, 207, 426, 672
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 6 cut(s) 57, 68, 87, 152, 365, 532
MseI TTAA 3 cut(s) 327, 429, 726
MspI CCGG 4 cut(s) 8, 15, 31, 499
MspR9I CCNGG 1 cut(s) 612
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 1 cut(s) 612
NdeII GATC 5 cut(s) 127, 161, 461, 544, 570
NlaIV GGNNCC 4 cut(s) 6, 98, 99, 436
NmeAIII GCCGAG 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 10
PaqCI CACCTGC 1 cut(s) 727
PfeI GAWTC 4 cut(s) 25, 394, 502, 638
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 3 cut(s) 70, 448, 451
PpuMI RGGWCCY 1 cut(s) 97
PshBI ATTAAT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 610
PspEI GGTNACC 1 cut(s) 10
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 4 cut(s) 6, 98, 99, 436
PspPI GGNCC 3 cut(s) 55, 97, 622
PspPPI RGGWCCY 1 cut(s) 97
PstI CTGCAG 1 cut(s) 451
PsuI RGATCY 2 cut(s) 161, 570
SaqAI TTAA 3 cut(s) 327, 429, 726
SatI GCNGC 3 cut(s) 69, 447, 450
Sau3AI GATC 5 cut(s) 127, 161, 461, 544, 570
Sau96I GGNCC 3 cut(s) 55, 97, 622
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 1 cut(s) 41
SetI ASST 8 cut(s) 60, 70, 275, 484, 536, 616, 721, 746
SfaNI GCATC 4 cut(s) 391, 500, 607, 657
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 3 cut(s) 55, 97, 622
Sse9I AATT 4 cut(s) 105, 207, 426, 672
SsiI CCGC 1 cut(s) 620
SspMI CTAG 3 cut(s) 189, 483, 626
StyD4I CCNGG 1 cut(s) 610
TaaI ACNGT 1 cut(s) 368
TaqI TCGA 2 cut(s) 23, 265
TasI AATT 4 cut(s) 105, 207, 426, 672
TfiI GAWTC 4 cut(s) 25, 394, 502, 638
Tru1I TTAA 3 cut(s) 327, 429, 726
Tru9I TTAA 3 cut(s) 327, 429, 726
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 68, 446, 449
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 287, 334, 429, 693
VpaK11BI GGWCC 3 cut(s) 55, 97, 622
VspI ATTAAT 1 cut(s) 327
XbaI TCTAGA 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 3 cut(s) 189, 483, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.