RchiOBHm_Chr5g0074251

COP9 signalosome complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004313
Physical Location & Seq
Forward (+)
80199235 .. 80199904
670 bp
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UTR
Exon/CDS
Intron
PRQ34907

Sequence Viewer

Length: 555 bp
ATGTTGTGGCCTTTTCATTTGGGTTATAAAATCGTGGGCCTCTCTAGCCTAGTTGCAGAATCGGCCCAGGCAACAATAGCGAGGCCATGGAACACTGTTACGAGCAAAAAAAGAAGCAGAGAGCTTGGAAAACCAGAAAGGCGAAAAAGCGAGAGAAAAAGAGAGAGAGAGAGAGAAAGCCTGAAACCCCCAAATCGGAGAAGAATGGCGGAGTCGTCGAGCAGTGGTCTCACGTTCAAGCTCCACCCTCTGGTCATGGTCAACATCTCCGACCACTACTCCCGCGTCAAGTCTCAGATGAACCCTCCCATCGCCGCCCACAACGCCACCGCAACAACCACCACCACCACCACCAACAACGGCGCCGAAGCCGTCGAAATGGCGTCGTCTTCCTCCTCCTCCTCCGCTTCGTCTCCGAGAGTCTTCGGCTGCGTCATCGGCGTCCAGCGTGGTCGCACCGTCGAGATCTTCAACAGCTTCGAGCTCCTCTACGATCCTGAGACTCACTCGCTCGAGCGTGGCTTCCTCGAGAAGAAGCAAGAGCTCTGTAGATAA

Protein Analysis

184

Amino Acids

20.61

Weight (kDa)

10.11

Isoelectric Point (pI)

56.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 75 - 172 3e-07 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 285
AciI CCGC 5 cut(s) 209, 283, 315, 330, 405
AclWI GGATC 1 cut(s) 488
AcyI GRCGYC 3 cut(s) 363, 383, 441
AfiI CCNNNNNNNGG 2 cut(s) 195, 250
AgsI TTSAA 2 cut(s) 238, 472
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 5 cut(s) 124, 241, 477, 484, 544
AluI AGCT 5 cut(s) 124, 241, 477, 484, 544
Alw21I GWGCWC 2 cut(s) 486, 546
Alw26I GTCTC 4 cut(s) 233, 297, 417, 494
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 2 cut(s) 512, 527
AoxI GGCC 4 cut(s) 8, 37, 63, 83
ApeKI GCWGC 1 cut(s) 429
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 37, 64
AvaI CYCGRG 2 cut(s) 512, 527
BanI GGYRCC 1 cut(s) 362
BanII GRGCYC 2 cut(s) 486, 546
BbsI GAAGAC 2 cut(s) 381, 415
Bbv12I GWGCWC 2 cut(s) 486, 546
BbvI GCAGC 1 cut(s) 416
BccI CCATC 1 cut(s) 317
BceAI ACGGC 2 cut(s) 356, 376
BciT130I CCWGG 1 cut(s) 68
BcoDI GTCTC 4 cut(s) 233, 297, 417, 494
BfaI CTAG 2 cut(s) 45, 50
BfmI CTRYAG 1 cut(s) 547
BfoI RGCGCY 1 cut(s) 366
BglII AGATCT 1 cut(s) 465
BisI GCNGC 2 cut(s) 315, 430
BlsI GCNGC 2 cut(s) 316, 431
Bme1390I CCNGG 1 cut(s) 68
BmeT110I CYCGRG 2 cut(s) 512, 527
BmgT120I GGNCC 2 cut(s) 37, 64
BmiI GGNNCC 1 cut(s) 364
BmrFI CCNGG 1 cut(s) 68
BpiI GAAGAC 2 cut(s) 381, 415
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BsaHI GRCGYC 3 cut(s) 363, 383, 441
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 2 cut(s) 66, 86
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 250
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 66, 86
BseLI CCNNNNNNNGG 2 cut(s) 195, 250
BseMII CTCAG 2 cut(s) 308, 489
BseRI GAGGAG 4 cut(s) 385, 388, 391, 476
BseXI GCAGC 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 4 cut(s) 10, 39, 65, 85
BshNI GGYRCC 1 cut(s) 362
BsiHKAI GWGCWC 2 cut(s) 486, 546
BsiHKCI CYCGRG 2 cut(s) 512, 527
BslI CCNNNNNNNGG 2 cut(s) 195, 250
BsmAI GTCTC 4 cut(s) 233, 297, 417, 494
BsmBI CGTCTC 1 cut(s) 417
BsnI GGCC 4 cut(s) 10, 39, 65, 85
Bso31I GGTCTC 1 cut(s) 233
BsoBI CYCGRG 2 cut(s) 512, 527
Bsp1286I GDGCHC 2 cut(s) 486, 546
Bsp143I GATC 2 cut(s) 465, 493
Bsp19I CCATGG 1 cut(s) 86
BspACI CCGC 5 cut(s) 209, 283, 315, 330, 405
BspANI GGCC 4 cut(s) 10, 39, 65, 85
BspCNI CTCAG 2 cut(s) 307, 490
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 364
BspPI GGATC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 66, 86
BssMI GATC 2 cut(s) 465, 493
BssNI GRCGYC 3 cut(s) 363, 383, 441
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 2 cut(s) 97, 460
BstACI GRCGYC 3 cut(s) 363, 383, 441
BstDEI CTNAG 2 cut(s) 294, 498
BstDSI CCRYGG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 2 cut(s) 468, 496
BstMAI GTCTC 4 cut(s) 233, 297, 417, 494
BstMBI GATC 2 cut(s) 465, 493
BstMWI GCNNNNNNNGC 5 cut(s) 45, 62, 77, 323, 438
BstNI CCWGG 1 cut(s) 68
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 547
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 416
BstV2I GAAGAC 2 cut(s) 381, 415
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BsuRI GGCC 4 cut(s) 10, 39, 65, 85
BtgI CCRYGG 1 cut(s) 86
BtgZI GCGATG 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 93, 229
CfoI GCGC 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 37, 64
CseI GACGC 4 cut(s) 274, 372, 421, 430
CviAII CATG 2 cut(s) 87, 256
DdeI CTNAG 2 cut(s) 294, 498
DinI GGCGCC 1 cut(s) 364
DpnI GATC 2 cut(s) 467, 495
DpnII GATC 2 cut(s) 465, 493
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 2 cut(s) 484, 544
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 2 cut(s) 486, 546
Eco31I GGTCTC 1 cut(s) 233
Eco53kI GAGCTC 2 cut(s) 484, 544
Eco88I CYCGRG 2 cut(s) 512, 527
EcoICRI GAGCTC 2 cut(s) 484, 544
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 1 cut(s) 86
EcoT38I GRGCYC 2 cut(s) 486, 546
EgeI GGCGCC 1 cut(s) 364
EheI GGCGCC 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 417
FaeI CATG 2 cut(s) 90, 259
FaiI YATR 3 cut(s) 27, 88, 257
FatI CATG 2 cut(s) 86, 255
FauI CCCGC 1 cut(s) 290
Fnu4HI GCNGC 2 cut(s) 315, 430
FriOI GRGCYC 2 cut(s) 486, 546
Fsp4HI GCNGC 2 cut(s) 315, 430
FspBI CTAG 2 cut(s) 45, 50
GlaI GCGC 1 cut(s) 364
GluI GCNGC 2 cut(s) 315, 430
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 4 cut(s) 10, 39, 65, 85
HgaI GACGC 4 cut(s) 274, 372, 421, 430
HhaI GCGC 1 cut(s) 365
Hin1I GRCGYC 3 cut(s) 363, 383, 441
Hin1II CATG 2 cut(s) 90, 259
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HinfI GANTC 4 cut(s) 59, 212, 420, 502
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 4 cut(s) 198, 271, 297, 417
Hpy188III TCNNGA 3 cut(s) 463, 497, 529
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 4 cut(s) 220, 377, 388, 464
HpyCH4III ACNGT 2 cut(s) 97, 460
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 1 cut(s) 56
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 62, 77, 323, 438
HpyF3I CTNAG 2 cut(s) 294, 498
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 3 cut(s) 363, 383, 441
Hsp92II CATG 2 cut(s) 90, 259
HspAI GCGC 1 cut(s) 363
KasI GGCGCC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 465, 493
LmnI GCTCC 2 cut(s) 246, 489
LpnPI CCDG 7 cut(s) 53, 80, 147, 194, 236, 458, 510
Lsp1109I GCAGC 1 cut(s) 416
MaeI CTAG 2 cut(s) 45, 50
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 97
MalI GATC 2 cut(s) 467, 495
MboI GATC 2 cut(s) 465, 493
MboII GAAGA 5 cut(s) 213, 381, 415, 460, 544
MflI RGATCY 1 cut(s) 465
MhlI GDGCHC 2 cut(s) 486, 546
Mly113I GGCGCC 1 cut(s) 363
MlyI GAGTC 3 cut(s) 221, 429, 496
MmeI TCCRAC 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 68
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 5 cut(s) 45, 62, 77, 323, 438
NarI GGCGCC 1 cut(s) 363
NcoI CCATGG 1 cut(s) 86
NdeII GATC 2 cut(s) 465, 493
NlaIII CATG 2 cut(s) 90, 259
NlaIV GGNNCC 1 cut(s) 364
PaeR7I CTCGAG 2 cut(s) 512, 527
PfeI GAWTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 2 cut(s) 316, 431
PleI GAGTC 3 cut(s) 220, 428, 496
PluTI GGCGCC 1 cut(s) 366
PpsI GAGTC 3 cut(s) 220, 428, 496
PsiI TTATAA 1 cut(s) 27
Psp124BI GAGCTC 2 cut(s) 486, 546
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 1 cut(s) 364
PspPI GGNCC 2 cut(s) 37, 64
PspXI VCTCGAGB 1 cut(s) 512
PsuI RGATCY 1 cut(s) 465
SacI GAGCTC 2 cut(s) 486, 546
SatI GCNGC 2 cut(s) 315, 430
Sau3AI GATC 2 cut(s) 465, 493
Sau96I GGNCC 2 cut(s) 37, 64
SchI GAGTC 3 cut(s) 221, 429, 496
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 486, 546
SetI ASST 6 cut(s) 126, 236, 243, 479, 486, 546
SfcI CTRYAG 1 cut(s) 547
SfoI GGCGCC 1 cut(s) 364
Sfr274I CTCGAG 2 cut(s) 512, 527
SlaI CTCGAG 2 cut(s) 512, 527
SmlI CTYRAG 2 cut(s) 512, 527
SmoI CTYRAG 2 cut(s) 512, 527
SsiI CCGC 5 cut(s) 209, 283, 315, 330, 405
SspDI GGCGCC 1 cut(s) 362
SspMI CTAG 2 cut(s) 45, 50
SstI GAGCTC 2 cut(s) 486, 546
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 2 cut(s) 97, 460
TaiI ACGT 1 cut(s) 236
TaqI TCGA 6 cut(s) 218, 375, 462, 480, 513, 528
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 1 cut(s) 59
TscAI CASTG 2 cut(s) 100, 229
TseI GCWGC 1 cut(s) 429
TspDTI ATGAA 2 cut(s) 5, 314
TspRI CASTG 2 cut(s) 100, 229
Van91I CCANNNNNTGG 1 cut(s) 250
XhoI CTCGAG 2 cut(s) 512, 527
XspI CTAG 2 cut(s) 45, 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.