RLG00000002225

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001813
Physical Location & Seq
Forward (+)
30072546 .. 30073240
695 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002225

Sequence Viewer

Length: 576 bp
ATGAAAACGGTGTTGCTCACCGCAACGCCAAGCCTCAGAACCTCGAAGGCACCGGAAACTGAAGGTTTTCGATTACGGACCCTCCGATTTGTCGGAGCAGCTCCAGGACGGCCTCCTTCACAAGTCTTGCGGGACGCCGGTGCACATGGAGCCGGAGGTTCTGTACCGGGTCGGGTGCGACAGATCCAAAGGCTGACTCGTGGTCCTGCGGCGTCATCCTCTTCGTTCTCTTCGCCGAACAGTTGTCCTCGACTTCTGGTCTTTCTCTCCCCGTCGCCACATGGATCTACCACCATCGCTGTCGTCGTGCCATGGCAGTGGCGTAGATCTGAAATTATGCTTCTCGGTTTTGGCTTTGGTTTTGTTTCGGTGTTTTGGAATGCATTTTGGACTTTGTTTCGTGGATTTGTGGCTGGACTGCTATTTTGCAATGCCATGGCTGTACTGAATGAGCACAGGTTTCTCAGTCTAATGGGGCTAACTCTGAAAGATGATGAAGCTGGGGATGAGAGGAAAAAGCTCATCACTTTCAAGTATTGGGTCACACGGATTTTACAGGCCGCCCGATGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.12

Weight (kDa)

11.49

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yos1 PF08571 137 - 189 1.9e-06 Yos1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 4 cut(s) 21, 130, 209, 561
AclWI GGATC 2 cut(s) 178, 292
AcuI CTGAAG 1 cut(s) 81
AcyI GRCGYC 2 cut(s) 135, 212
AfaI GTAC 2 cut(s) 165, 444
AfiI CCNNNNNNNGG 1 cut(s) 567
AgsI TTSAA 1 cut(s) 532
AhdI GACNNNNNGTC 2 cut(s) 201, 257
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 3 cut(s) 101, 500, 520
AluI AGCT 3 cut(s) 101, 500, 520
Alw21I GWGCWC 2 cut(s) 145, 456
Alw44I GTGCAC 1 cut(s) 141
AlwI GGATC 2 cut(s) 178, 292
AoxI GGCC 2 cut(s) 110, 558
ApaLI GTGCAC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 408, 440
Asp700I GAANNNNTTC 1 cut(s) 66
AspS9I GGNCC 2 cut(s) 78, 203
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 1 cut(s) 10
AvaII GGWCC 2 cut(s) 78, 203
BaeGI GKGCMC 1 cut(s) 145
BanI GGYRCC 1 cut(s) 49
BauI CACGAG 1 cut(s) 198
Bbv12I GWGCWC 2 cut(s) 145, 456
BbvI GCAGC 1 cut(s) 110
BccI CCATC 2 cut(s) 302, 561
BceAI ACGGC 1 cut(s) 125
BciT130I CCWGG 1 cut(s) 105
BcnI CCSGG 1 cut(s) 168
BglII AGATCT 1 cut(s) 326
BisI GCNGC 3 cut(s) 99, 210, 561
BlsI GCNGC 3 cut(s) 100, 211, 562
Bme1390I CCNGG 2 cut(s) 105, 168
Bme18I GGWCC 2 cut(s) 78, 203
BmeRI GACNNNNNGTC 2 cut(s) 201, 257
BmgT120I GGNCC 2 cut(s) 78, 203
BmiI GGNNCC 3 cut(s) 51, 80, 151
BmrFI CCNGG 2 cut(s) 105, 168
BpmI CTGGAG 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 135, 212
BsaJI CCNNGG 2 cut(s) 311, 435
BsaWI WCCGGW 1 cut(s) 52
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 147, 177
Bsc4I CCNNNNNNNGG 1 cut(s) 567
Bse118I RCCGGY 1 cut(s) 137
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 311, 435
BseGI GGATG 2 cut(s) 215, 511
BseLI CCNNNNNNNGG 1 cut(s) 567
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 2 cut(s) 49, 478
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 110
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 2 cut(s) 112, 560
BshNI GGYRCC 1 cut(s) 49
BsiHKAI GWGCWC 2 cut(s) 145, 456
BsiSI CCGG 4 cut(s) 53, 138, 153, 167
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 567
BsmFI GGGAC 1 cut(s) 146
BsmI GAATGC 1 cut(s) 385
BsnI GGCC 2 cut(s) 112, 560
Bsp1286I GDGCHC 2 cut(s) 145, 456
Bsp143I GATC 3 cut(s) 183, 284, 326
Bsp19I CCATGG 2 cut(s) 311, 435
BspACI CCGC 4 cut(s) 21, 130, 209, 561
BspANI GGCC 2 cut(s) 112, 560
BspCNI CTCAG 2 cut(s) 48, 477
BspLI GGNNCC 3 cut(s) 51, 80, 151
BspPI GGATC 2 cut(s) 178, 292
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 311, 435
BssMI GATC 3 cut(s) 183, 284, 326
BssNI GRCGYC 2 cut(s) 135, 212
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 311, 435
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 10, 242
Bst6I CTCTTC 2 cut(s) 226, 235
BstACI GRCGYC 2 cut(s) 135, 212
BstDEI CTNAG 2 cut(s) 35, 464
BstDSI CCRYGG 2 cut(s) 311, 435
BstF5I GGATG 2 cut(s) 215, 511
BstKTI GATC 3 cut(s) 186, 287, 329
BstMBI GATC 3 cut(s) 183, 284, 326
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 103, 166
BstSLI GKGCMC 1 cut(s) 145
BstV1I GCAGC 1 cut(s) 110
BstX2I RGATCY 3 cut(s) 183, 284, 326
BstXI CCANNNNNNTGG 1 cut(s) 318
BstYI RGATCY 3 cut(s) 183, 284, 326
BsuRI GGCC 2 cut(s) 112, 560
BtgI CCRYGG 2 cut(s) 311, 435
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 215, 511
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 78, 203
CseI GACGC 2 cut(s) 143, 201
Csp6I GTAC 2 cut(s) 164, 443
CviAII CATG 4 cut(s) 146, 281, 312, 436
CviQI GTAC 2 cut(s) 164, 443
DdeI CTNAG 2 cut(s) 35, 464
DpnI GATC 3 cut(s) 185, 286, 328
DpnII GATC 3 cut(s) 183, 284, 326
DriI GACNNNNNGTC 2 cut(s) 201, 257
Eam1104I CTCTTC 2 cut(s) 226, 235
Eam1105I GACNNNNNGTC 2 cut(s) 201, 257
EarI CTCTTC 2 cut(s) 226, 235
Eco130I CCWWGG 2 cut(s) 311, 435
Eco47I GGWCC 2 cut(s) 78, 203
Eco57I CTGAAG 1 cut(s) 81
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 311, 435
EcoT22I ATGCAT 1 cut(s) 385
ErhI CCWWGG 2 cut(s) 311, 435
FaeI CATG 4 cut(s) 149, 284, 315, 439
FaiI YATR 5 cut(s) 147, 282, 313, 338, 437
FaqI GGGAC 1 cut(s) 146
FatI CATG 4 cut(s) 145, 280, 311, 435
FauI CCCGC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 99, 210, 561
FokI GGATG 2 cut(s) 202, 518
Fsp4HI GCNGC 3 cut(s) 99, 210, 561
GluI GCNGC 3 cut(s) 99, 210, 561
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 87
HaeIII GGCC 2 cut(s) 112, 560
HapII CCGG 4 cut(s) 53, 138, 153, 167
HgaI GACGC 2 cut(s) 143, 201
Hin1I GRCGYC 2 cut(s) 135, 212
Hin1II CATG 4 cut(s) 149, 284, 315, 439
HinfI GANTC 1 cut(s) 196
HpaII CCGG 4 cut(s) 53, 138, 153, 167
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 5 cut(s) 38, 86, 95, 331, 486
Hpy8I GTNNAC 1 cut(s) 143
Hpy99I CGWCG 2 cut(s) 277, 308
HpyAV CCTTC 3 cut(s) 40, 56, 126
HpyCH4III ACNGT 2 cut(s) 10, 242
HpyCH4V TGCA 3 cut(s) 143, 383, 429
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 2 cut(s) 35, 464
Hsp92I GRCGYC 2 cut(s) 135, 212
Hsp92II CATG 4 cut(s) 149, 284, 315, 439
Kzo9I GATC 3 cut(s) 183, 284, 326
LmnI GCTCC 3 cut(s) 95, 106, 149
Lsp1109I GCAGC 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 541
MalI GATC 3 cut(s) 185, 286, 328
MboI GATC 3 cut(s) 183, 284, 326
MboII GAAGA 2 cut(s) 213, 222
MflI RGATCY 3 cut(s) 183, 284, 326
MhlI GDGCHC 2 cut(s) 145, 456
MluCI AATT 1 cut(s) 333
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 73
MnlI CCTC 8 cut(s) 44, 52, 92, 123, 149, 229, 258, 504
Mph1103I ATGCAT 1 cut(s) 385
MroXI GAANNNNTTC 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 316
MspI CCGG 4 cut(s) 53, 138, 153, 167
MspR9I CCNGG 2 cut(s) 105, 168
Mva1269I GAATGC 1 cut(s) 385
MvaI CCWGG 1 cut(s) 105
MwoI GCNNNNNNNGC 1 cut(s) 149
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 2 cut(s) 311, 435
NdeII GATC 3 cut(s) 183, 284, 326
NlaIII CATG 4 cut(s) 149, 284, 315, 439
NlaIV GGNNCC 3 cut(s) 51, 80, 151
NmuCI GTSAC 1 cut(s) 541
NsiI ATGCAT 1 cut(s) 385
PcsI WCGNNNNNNNCGW 2 cut(s) 82, 303
PctI GAATGC 1 cut(s) 385
PdmI GAANNNNTTC 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 103
PkrI GCNGC 3 cut(s) 100, 211, 562
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 3 cut(s) 51, 80, 151
PspPI GGNCC 2 cut(s) 78, 203
PsuI RGATCY 3 cut(s) 183, 284, 326
RsaI GTAC 2 cut(s) 165, 444
RsaNI GTAC 2 cut(s) 164, 443
RseI CAYNNNNRTG 1 cut(s) 316
SatI GCNGC 3 cut(s) 99, 210, 561
Sau3AI GATC 3 cut(s) 183, 284, 326
Sau96I GGNCC 2 cut(s) 78, 203
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 105, 168
SduI GDGCHC 2 cut(s) 145, 456
SetI ASST 7 cut(s) 44, 67, 103, 160, 461, 502, 522
SgrAI CRCCGGYG 1 cut(s) 137
SinI GGWCC 2 cut(s) 78, 203
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 1 cut(s) 333
SsiI CCGC 4 cut(s) 21, 130, 209, 561
StyD4I CCNGG 2 cut(s) 103, 166
StyI CCWWGG 2 cut(s) 311, 435
TaaI ACNGT 2 cut(s) 10, 242
TaqI TCGA 3 cut(s) 44, 70, 250
TasI AATT 1 cut(s) 333
TatI WGTACW 1 cut(s) 442
TauI GCSGC 2 cut(s) 212, 563
TscAI CASTG 1 cut(s) 323
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 2 cut(s) 17, 510
TspGWI ACGGA 2 cut(s) 91, 562
TspRI CASTG 1 cut(s) 323
VneI GTGCAC 1 cut(s) 141
VpaK11BI GGWCC 2 cut(s) 78, 203
XmnI GAANNNNTTC 1 cut(s) 66
Zsp2I ATGCAT 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.