RLG00000008118

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002883
Physical Location & Seq
Reverse (-)
25880168 .. 25882873
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008118

Sequence Viewer

Length: 927 bp
ATGGCGCAGATGGTTGATGAAAAGAAGAAATGGACGATTACATTTGCGAGAGAGTTGAAGAAGGAGGGTATAATAGCTGCACCAATGGTGGCAGTGACGGCACTGCAGTATCTTCTGCAACTAGTATCGATGATTATGGTCGGACATCTTGATCAGCTCTCCCTCTCCAGTGTTGCCATTGCCATTTCTCTCACCAATGTCACTGGTTTCAGCCTTCTTTCAGGATTGGCAGGTGGATTGGAAACCTTATGTGGGCAAGCATTTGGAGCAGAGCAATACCAAAAACTTGGAACTTATACTTGTACTGCTATGATATCACTTCTCTTGGTTTGTCCTCCAGTATGTCTCCTATGGATATTCATGGATAAATTGTTACCTTTAGTAGGCCAGGATCCATTGATATCCCTTCAGGCACGCAAATATTCAATGTGGCTTATCCCTGCACTATTTGGTGCATCAATTCTTAAACCGTTAACTCGCTACTCCCAGTCTCAGAGTCTTATTTTCCCAATGCTCCTATCCTCTTTTGCCATTCTTCTCTTCCATATATCTGCCTCATGGACTCTTGTGTATAAGCTGGAATTTGGAAGTAAAGGTTCAGCAATAGCCTTCAGTTTATCCACTTGGTTATATGTGGTAATTCTGGGTTCCTATGCAATGTATTCCTCAGTCTTTGAGAAAACCCGTATTAGAATTTCAAAAGATTCTATCCTTCATGTTGGAGAGTTCTTTTGCTTGGCTATCCCGTCTGCTGTAATGGTTTGTCTAAAATGGTGGTCGTGCGAGCTGCTTATTTTTCTGTCCGGCCTTCTACCAAATCCTAAACTAGAGACTTCACCTTACAATCTCTACTTTGCACTTCACTTTATCACATGGATTCGGAGCTGCTGCAAGTTTGGTATTATGTTAGTAATGCCTTATTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

309

Amino Acids

34.36

Weight (kDa)

8.89

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 28 - 187 1.7e-27 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 221
AclWI GGATC 2 cut(s) 386, 399
AcsI RAATTY 2 cut(s) 581, 693
AcuI CTGAAG 2 cut(s) 392, 595
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 2 cut(s) 252, 383
AgsI TTSAA 3 cut(s) 58, 426, 699
AhlI ACTAGT 1 cut(s) 121
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 5 cut(s) 77, 157, 577, 787, 885
AluI AGCT 5 cut(s) 77, 157, 577, 787, 885
Alw26I GTCTC 3 cut(s) 350, 495, 824
AlwI GGATC 2 cut(s) 386, 399
AoxI GGCC 2 cut(s) 385, 805
ApeKI GCWGC 4 cut(s) 77, 787, 885, 888
ApoI RAATTY 2 cut(s) 581, 693
AspLEI GCGC 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 184, 828
BaeI ACNNNNGTAYC 2 cut(s) 92, 125
BamHI GGATCC 1 cut(s) 391
BbvI GCAGC 4 cut(s) 64, 774, 872, 875
BccI CCATC 1 cut(s) 4
BceAI ACGGC 1 cut(s) 114
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 3 cut(s) 350, 495, 824
BcuI ACTAGT 1 cut(s) 121
BfaI CTAG 2 cut(s) 122, 827
BfmI CTRYAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 221
BisI GCNGC 4 cut(s) 78, 788, 886, 889
BlsI GCNGC 4 cut(s) 79, 789, 887, 890
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 2 cut(s) 393, 649
BmrFI CCNGG 1 cut(s) 389
BmrI ACTGGG 1 cut(s) 481
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 481
BpmI CTGGAG 2 cut(s) 151, 321
Bsa29I ATCGAT 1 cut(s) 128
Bsc4I CCNNNNNNNGG 2 cut(s) 252, 383
Bse1I ACTGG 4 cut(s) 168, 208, 338, 487
Bse3DI GCAATG 2 cut(s) 177, 663
BseBI CCWGG 1 cut(s) 389
BseCI ATCGAT 1 cut(s) 128
BseLI CCNNNNNNNGG 2 cut(s) 252, 383
BseMI GCAATG 2 cut(s) 177, 663
BseMII CTCAG 2 cut(s) 506, 681
BseNI ACTGG 4 cut(s) 168, 208, 338, 487
BseXI GCAGC 4 cut(s) 64, 774, 872, 875
BsgI GTGCAG 2 cut(s) 63, 426
BshFI GGCC 2 cut(s) 387, 807
BshVI ATCGAT 1 cut(s) 128
BsiSI CCGG 1 cut(s) 804
BslI CCNNNNNNNGG 2 cut(s) 252, 383
BsmAI GTCTC 3 cut(s) 350, 495, 824
BsnI GGCC 2 cut(s) 387, 807
Bsp143I GATC 2 cut(s) 151, 391
BspANI GGCC 2 cut(s) 387, 807
BspCNI CTCAG 2 cut(s) 505, 680
BspDI ATCGAT 1 cut(s) 128
BspLI GGNNCC 2 cut(s) 393, 649
BspMAI CTGCAG 1 cut(s) 108
BspMI ACCTGC 1 cut(s) 221
BspPI GGATC 2 cut(s) 386, 399
BsrDI GCAATG 2 cut(s) 177, 663
BsrI ACTGG 4 cut(s) 168, 208, 338, 487
BssMI GATC 2 cut(s) 151, 391
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 258, 415, 785
BstDEI CTNAG 2 cut(s) 492, 667
BstENI CCTNNNNNAGG 1 cut(s) 381
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 154, 394
BstMAI GTCTC 3 cut(s) 350, 495, 824
BstMBI GATC 2 cut(s) 151, 391
BstMWI GCNNNNNNNGC 2 cut(s) 98, 266
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstSFI CTRYAG 1 cut(s) 104
BstV1I GCAGC 4 cut(s) 64, 774, 872, 875
BstX2I RGATCY 1 cut(s) 391
BstXI CCANNNNNNTGG 1 cut(s) 287
BstYI RGATCY 1 cut(s) 391
Bsu15I ATCGAT 1 cut(s) 128
BsuRI GGCC 2 cut(s) 387, 807
BsuTUI ATCGAT 1 cut(s) 128
BtsI GCAGTG 2 cut(s) 99, 101
BtsIMutI CAGTG 4 cut(s) 99, 101, 175, 201
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 3 cut(s) 258, 415, 785
CfoI GCGC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 128
Csp6I GTAC 1 cut(s) 303
CviAII CATG 4 cut(s) 361, 558, 716, 873
CviQI GTAC 1 cut(s) 303
DdeI CTNAG 2 cut(s) 492, 667
DpnI GATC 2 cut(s) 153, 393
DpnII GATC 2 cut(s) 151, 391
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco32I GATATC 2 cut(s) 315, 402
Eco57I CTGAAG 2 cut(s) 392, 595
EcoNI CCTNNNNNAGG 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 387
EcoRV GATATC 2 cut(s) 315, 402
FaeI CATG 4 cut(s) 364, 561, 719, 876
FatI CATG 4 cut(s) 360, 557, 715, 872
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 4 cut(s) 78, 788, 886, 889
Fsp4HI GCNGC 4 cut(s) 78, 788, 886, 889
FspBI CTAG 2 cut(s) 122, 827
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 78, 788, 886, 889
GsuI CTGGAG 2 cut(s) 151, 321
HaeIII GGCC 2 cut(s) 387, 807
HapII CCGG 1 cut(s) 804
HhaI GCGC 1 cut(s) 7
Hin1II CATG 4 cut(s) 364, 561, 719, 876
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 4 cut(s) 496, 562, 704, 877
HpaI GTTAAC 1 cut(s) 474
HpaII CCGG 1 cut(s) 804
HphI GGTGA 2 cut(s) 184, 828
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 3 cut(s) 143, 495, 882
Hpy188III TCNNGA 2 cut(s) 149, 222
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 6 cut(s) 55, 224, 416, 619, 722, 818
HpyCH4III ACNGT 1 cut(s) 471
HpyCH4V TGCA 8 cut(s) 80, 106, 118, 443, 455, 656, 857, 891
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 266
HpyF3I CTNAG 2 cut(s) 492, 667
Hsp92II CATG 4 cut(s) 364, 561, 719, 876
HspAI GCGC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 151
KspAI GTTAAC 1 cut(s) 474
Kzo9I GATC 2 cut(s) 151, 391
LmnI GCTCC 3 cut(s) 266, 519, 882
Lsp1109I GCAGC 4 cut(s) 64, 774, 872, 875
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 122, 827
MaeIII GTNAC 3 cut(s) 94, 199, 372
MalI GATC 2 cut(s) 153, 393
MboI GATC 2 cut(s) 151, 391
MboII GAAGA 5 cut(s) 37, 70, 104, 527, 532
MflI RGATCY 1 cut(s) 391
MluCI AATT 5 cut(s) 368, 459, 581, 639, 693
MlyI GAGTC 2 cut(s) 505, 556
MmeI TCCRAC 2 cut(s) 121, 700
MnlI CCTC 6 cut(s) 58, 173, 345, 532, 565, 676
MseI TTAA 2 cut(s) 465, 473
MspI CCGG 1 cut(s) 804
MspR9I CCNGG 1 cut(s) 389
MvaI CCWGG 1 cut(s) 389
MwoI GCNNNNNNNGC 2 cut(s) 98, 266
NdeII GATC 2 cut(s) 151, 391
NlaIII CATG 4 cut(s) 364, 561, 719, 876
NlaIV GGNNCC 2 cut(s) 393, 649
NmuCI GTSAC 2 cut(s) 94, 199
PaqCI CACCTGC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 704, 877
PkrI GCNGC 4 cut(s) 79, 789, 887, 890
PleI GAGTC 2 cut(s) 504, 556
PpsI GAGTC 2 cut(s) 504, 556
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 393, 649
PstI CTGCAG 1 cut(s) 108
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 465, 473
SatI GCNGC 4 cut(s) 78, 788, 886, 889
Sau3AI GATC 2 cut(s) 151, 391
SchI GAGTC 2 cut(s) 505, 556
ScrFI CCNGG 1 cut(s) 389
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 104
SpeI ACTAGT 1 cut(s) 121
Sse9I AATT 5 cut(s) 368, 459, 581, 639, 693
SspI AATATT 1 cut(s) 422
SspMI CTAG 2 cut(s) 122, 827
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 471
TaqI TCGA 1 cut(s) 128
TasI AATT 5 cut(s) 368, 459, 581, 639, 693
TatI WGTACW 1 cut(s) 302
TfiI GAWTC 2 cut(s) 704, 877
Tru1I TTAA 2 cut(s) 465, 473
Tru9I TTAA 2 cut(s) 465, 473
TscAI CASTG 4 cut(s) 99, 108, 175, 208
TseFI GTSAC 2 cut(s) 94, 199
TseI GCWGC 4 cut(s) 77, 787, 885, 888
Tsp45I GTSAC 2 cut(s) 94, 199
TspDTI ATGAA 3 cut(s) 33, 349, 704
TspRI CASTG 4 cut(s) 99, 108, 175, 208
XagI CCTNNNNNAGG 1 cut(s) 381
XapI RAATTY 2 cut(s) 581, 693
XspI CTAG 2 cut(s) 122, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.