Rroxscaffold_5G00358570

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002883
Physical Location & Seq
Forward (+)
38635143 .. 38637445
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358570.1

Sequence Viewer

Length: 786 bp
ATGCAACAGAAACTAGAAATGGGACAGATGGTTGATGAAAAGAAGAAATGGACGATTACATTTGCGAGAGAGTTGAAGGAGGAGGGTATAATAGCTGCACCAATGGTGGCAGTGACGGCACTGCAGTATCTTCTGCAACTAGTATCGATGATTATGGTCGGACATCTTGATCAGCTCTCCCTCTCCAGTGTTGCAATTGCCATTTCTCTCACCAATGTCACTGGTTTCAGCCTTCTTTCAGGATTGGCAGGTGGATTGGAAACCTTATGTGGGCAAGCATTTGGAGCAGAGCAATACCAAAAACTTGGAACTTATACTTGTACTGCTATCATATCACTTCTCTTGGTTTGTCCTCCAGTATGTCTCCTATGGATATTCATGGATAAATTGTTACCTTTAGTAGGCCAGGATCCATTGATTTCCCTTCAGGCACGCAAATATTCAATGTGGCTTATCCCTGCACTATTTGGTGCATCAATTCTTAAACCGTTAACTCGCTACTCCCAGTCTCAGAGTCTTATTTTCCCAATGCTCCTATCCTCTTTTGCCATTCTTCTCTTCCATATATCTGCCTCATGGGCTCTTGTGTATAAGCTGGAATTTGGAAGTAAAGGTTCAGCAATAGCCTTCAGTTTATCCACTTGGTTATATGTGGTAATGCTGGGTTCCTATGCAATGTATTCCTCAGTCTTTGAGAAAACCCGTATTAGAATTTCAAAAGATTCCATCCTTCATGTTGGAGAGTTCTTTTGCTTGGCCATCCCGTCTGCTGTAATGGTTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

261

Amino Acids

28.82

Weight (kDa)

8.54

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 34 - 192 7.2e-28 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 239
AclWI GGATC 2 cut(s) 404, 417
AcoI YGGCCR 1 cut(s) 756
AcsI RAATTY 2 cut(s) 599, 711
AcuI CTGAAG 2 cut(s) 410, 613
AfaI GTAC 1 cut(s) 322
AfiI CCNNNNNNNGG 2 cut(s) 270, 401
AgsI TTSAA 3 cut(s) 76, 444, 717
AhlI ACTAGT 1 cut(s) 139
AjnI CCWGG 1 cut(s) 405
AluBI AGCT 3 cut(s) 95, 175, 595
AluI AGCT 3 cut(s) 95, 175, 595
Alw26I GTCTC 2 cut(s) 368, 513
AlwI GGATC 2 cut(s) 404, 417
AoxI GGCC 2 cut(s) 403, 756
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 2 cut(s) 599, 711
AsuHPI GGTGA 1 cut(s) 202
BaeI ACNNNNGTAYC 2 cut(s) 110, 143
BalI TGGCCA 1 cut(s) 758
BamHI GGATCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 583
BbvI GCAGC 1 cut(s) 82
BccI CCATC 3 cut(s) 22, 734, 767
BceAI ACGGC 1 cut(s) 132
BciT130I CCWGG 1 cut(s) 407
BclI TGATCA 1 cut(s) 169
BcoDI GTCTC 2 cut(s) 368, 513
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 2 cut(s) 14, 140
BfmI CTRYAG 1 cut(s) 122
BfuAI ACCTGC 1 cut(s) 239
BglI GCCNNNNNGGC 1 cut(s) 578
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 407
BmiI GGNNCC 2 cut(s) 411, 667
BmrFI CCNGG 1 cut(s) 407
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 482
BmuI ACTGGG 1 cut(s) 499
BpmI CTGGAG 2 cut(s) 169, 339
Bsa29I ATCGAT 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 401
Bse1I ACTGG 4 cut(s) 186, 226, 356, 505
Bse3DI GCAATG 1 cut(s) 681
BseBI CCWGG 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 146
BseGI GGATG 2 cut(s) 726, 759
BseLI CCNNNNNNNGG 2 cut(s) 270, 401
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 2 cut(s) 524, 699
BseNI ACTGG 4 cut(s) 186, 226, 356, 505
BseRI GAGGAG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 2 cut(s) 81, 444
BshFI GGCC 2 cut(s) 405, 758
BshVI ATCGAT 1 cut(s) 146
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 270, 401
BsmAI GTCTC 2 cut(s) 368, 513
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 2 cut(s) 405, 758
Bsp1286I GDGCHC 1 cut(s) 583
Bsp143I GATC 2 cut(s) 169, 409
BspANI GGCC 2 cut(s) 405, 758
BspCNI CTCAG 2 cut(s) 523, 698
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 2 cut(s) 411, 667
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 2 cut(s) 404, 417
BsrDI GCAATG 1 cut(s) 681
BsrI ACTGG 4 cut(s) 186, 226, 356, 505
BssMI GATC 2 cut(s) 169, 409
Bst2UI CCWGG 1 cut(s) 407
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 276, 433
BstDEI CTNAG 2 cut(s) 510, 685
BstENI CCTNNNNNAGG 1 cut(s) 399
BstF5I GGATG 2 cut(s) 726, 759
BstKTI GATC 2 cut(s) 172, 412
BstMAI GTCTC 2 cut(s) 368, 513
BstMBI GATC 2 cut(s) 169, 409
BstMWI GCNNNNNNNGC 3 cut(s) 116, 284, 578
BstNI CCWGG 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 405
BstSFI CTRYAG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 305
BstYI RGATCY 1 cut(s) 409
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 2 cut(s) 405, 758
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 2 cut(s) 726, 759
BtsI GCAGTG 2 cut(s) 117, 119
BtsIMutI CAGTG 4 cut(s) 117, 119, 193, 219
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 276, 433
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 1 cut(s) 321
CviAII CATG 3 cut(s) 379, 576, 734
CviJI RGCY 9 cut(s) 95, 175, 231, 405, 451, 581, 595, 626, 758
CviKI_1 RGCY 9 cut(s) 95, 175, 231, 405, 451, 581, 595, 626, 758
CviQI GTAC 1 cut(s) 321
DdeI CTNAG 2 cut(s) 510, 685
DpnI GATC 2 cut(s) 171, 411
DpnII GATC 2 cut(s) 169, 409
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco24I GRGCYC 1 cut(s) 583
Eco57I CTGAAG 2 cut(s) 410, 613
EcoNI CCTNNNNNAGG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 583
FaeI CATG 3 cut(s) 382, 579, 737
FaqI GGGAC 1 cut(s) 36
FatI CATG 3 cut(s) 378, 575, 733
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 96
FokI GGATG 2 cut(s) 713, 746
FriOI GRGCYC 1 cut(s) 583
Fsp4HI GCNGC 1 cut(s) 96
FspBI CTAG 2 cut(s) 14, 140
GluI GCNGC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 2 cut(s) 169, 339
HaeIII GGCC 2 cut(s) 405, 758
Hin1II CATG 3 cut(s) 382, 579, 737
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 2 cut(s) 514, 722
HpaI GTTAAC 1 cut(s) 492
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 492
Hpy188I TCNGA 2 cut(s) 161, 513
Hpy188III TCNNGA 2 cut(s) 167, 240
Hpy8I GTNNAC 1 cut(s) 492
HpyAV CCTTC 5 cut(s) 70, 242, 434, 637, 740
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4V TGCA 8 cut(s) 4, 98, 124, 136, 194, 461, 473, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 284, 578
HpyF3I CTNAG 2 cut(s) 510, 685
Hsp92II CATG 3 cut(s) 382, 579, 737
Ksp22I TGATCA 1 cut(s) 169
KspAI GTTAAC 1 cut(s) 492
Kzo9I GATC 2 cut(s) 169, 409
LmnI GCTCC 2 cut(s) 284, 537
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 1 cut(s) 482
MaeI CTAG 2 cut(s) 14, 140
MaeIII GTNAC 3 cut(s) 112, 217, 390
MalI GATC 2 cut(s) 171, 411
MboI GATC 2 cut(s) 169, 409
MboII GAAGA 4 cut(s) 55, 122, 545, 550
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 583
MlsI TGGCCA 1 cut(s) 758
MluCI AATT 5 cut(s) 195, 386, 477, 599, 711
MluNI TGGCCA 1 cut(s) 758
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 2 cut(s) 139, 718
MnlI CCTC 7 cut(s) 73, 76, 191, 363, 550, 583, 694
Mox20I TGGCCA 1 cut(s) 758
MscI TGGCCA 1 cut(s) 758
MseI TTAA 2 cut(s) 483, 491
Msp20I TGGCCA 1 cut(s) 758
MspR9I CCNGG 1 cut(s) 407
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 407
MwoI GCNNNNNNNGC 3 cut(s) 116, 284, 578
NdeII GATC 2 cut(s) 169, 409
NlaIII CATG 3 cut(s) 382, 579, 737
NlaIV GGNNCC 2 cut(s) 411, 667
NmuCI GTSAC 2 cut(s) 112, 217
PaqCI CACCTGC 1 cut(s) 239
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 1 cut(s) 97
PleI GAGTC 1 cut(s) 522
PpsI GAGTC 1 cut(s) 522
Psp6I CCWGG 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 1 cut(s) 405
PspN4I GGNNCC 2 cut(s) 411, 667
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 1 cut(s) 322
RsaNI GTAC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 483, 491
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 2 cut(s) 169, 409
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 1 cut(s) 407
SduI GDGCHC 1 cut(s) 583
SetI ASST 7 cut(s) 97, 177, 253, 266, 397, 597, 616
SfaNI GCATC 1 cut(s) 482
SfcI CTRYAG 1 cut(s) 122
SpeI ACTAGT 1 cut(s) 139
Sse9I AATT 5 cut(s) 195, 386, 477, 599, 711
SspI AATATT 1 cut(s) 440
SspMI CTAG 2 cut(s) 14, 140
StyD4I CCNGG 1 cut(s) 405
TaaI ACNGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 146
TasI AATT 5 cut(s) 195, 386, 477, 599, 711
TatI WGTACW 1 cut(s) 320
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 2 cut(s) 483, 491
Tru9I TTAA 2 cut(s) 483, 491
TscAI CASTG 4 cut(s) 117, 126, 193, 226
TseFI GTSAC 2 cut(s) 112, 217
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 2 cut(s) 112, 217
TspDTI ATGAA 3 cut(s) 51, 367, 722
TspRI CASTG 4 cut(s) 117, 126, 193, 226
XagI CCTNNNNNAGG 1 cut(s) 399
XapI RAATTY 2 cut(s) 599, 711
XspI CTAG 2 cut(s) 14, 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.