Rroxscaffold_5G00358590

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002883
Physical Location & Seq
Forward (+)
38660752 .. 38668123
7372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00358590.1

Sequence Viewer

Length: 1047 bp
ATGCAGCCGAAACTAGAAAAGGGACAGATGGTTGATGAAGATAAGAAATGGACGATTACATTTGCGAGAGAGTTGAAGAAGGAGGGTATTATAGCTGCACCAATGGTTGCAGTGACGGTACTGCAGTATCTTTCGCAACTAGTATCGATGATTATGGTCGGACATCTTGGTCACCGGTTTCACCTTCTTCAGTCTGGATTGGCAGGTGGATTGGAAACCTTATGTGGGCAAGCATTTGGAGCAGAGCAATACCAAAAACTTGGAACTTATACTTGTACTGCTATGATATCACTTCTCTTGGTTTGTCCTCCAGTATGTCTCCTATGGATATTCATGGATAAATTTTTACCTTTATTAGGCCAGGATCCATTGATACCCCTTCAGGCACGCAAATATTCAATGTGGCTTATTCCTGCACTATTTGGTGCTTCAATTATTAAACCGTTAACTCGCTACTTCCAGTCTCAGAGTCTCATTTTCCCAATGCTCCTATCCTCTTTTGCCATTCTTCTCTTCCATATATCTGCCTCATGGGCTCTTGTGTATAAGCTGGGATTTGGAAGTAAAGGTTCAGCAATAGCCTTCAGTTTATCCACTTGGTTATATGTGGTAATGCTGGGTTCCTATGCAATGTATTCCTCAGTCTTCGAGAAAACCCGTATTACAATTTCAAAAGATTCTATTCTTCATGTTGGAGAGTTCTTTCGCTTGGCTATCCCGTCTGCTGTAATGGTTTGCACATATTATGTCACCATAAGAATACATATCTGTGAAGGGGTAGCTAGAGGAAGTGGATGGCAGCATTTGGGGGCGTATGTCAATCTTGGGGCATTTTATTTGGTTGGACTTCCTGTGGCTATGGTACTCGGGTTTCCTGTGCACTTAAGGGGGAAAGGCCTTTGGATTGGAATAGTGGTCGGCTCCGCTGTACAATCAACTCTTCTGGGTTGCATCACCTGTTTCACAAATTGGATTATACAGGCAAAGTTGGCAAGAGATAGGATATTCACAGAGAGATCATCATCAACCGGGGGCGGAGACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

348

Amino Acids

38.39

Weight (kDa)

9.29

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 65 - 177 4.1e-16 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
AasI GACNNNNNNGTC 1 cut(s) 168
Acc36I ACCTGC 1 cut(s) 194
AciI CCGC 2 cut(s) 924, 1035
AclWI GGATC 2 cut(s) 359, 372
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 3 cut(s) 173, 365, 568
AfaI GTAC 4 cut(s) 120, 277, 864, 930
AfiI CCNNNNNNNGG 2 cut(s) 225, 356
AflII CTTAAG 1 cut(s) 883
AgeI ACCGGT 1 cut(s) 174
AgsI TTSAA 4 cut(s) 76, 399, 432, 672
AhlI ACTAGT 1 cut(s) 139
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 3 cut(s) 95, 550, 782
AluI AGCT 3 cut(s) 95, 550, 782
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 4 cut(s) 323, 468, 476, 1032
Alw44I GTGCAC 1 cut(s) 878
AlwI GGATC 2 cut(s) 359, 372
Ama87I CYCGRG 1 cut(s) 866
AoxI GGCC 2 cut(s) 358, 895
ApaLI GTGCAC 1 cut(s) 878
ApeKI GCWGC 3 cut(s) 4, 95, 799
ApoI RAATTY 1 cut(s) 341
AsiGI ACCGGT 1 cut(s) 174
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 4 cut(s) 164, 173, 742, 946
AvaI CYCGRG 1 cut(s) 866
BaeGI GKGCMC 1 cut(s) 882
BaeI ACNNNNGTAYC 6 cut(s) 110, 110, 143, 143, 854, 887
BamHI GGATCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 637
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 3 cut(s) 16, 82, 811
BccI CCATC 2 cut(s) 22, 789
BciT130I CCWGG 1 cut(s) 362
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 4 cut(s) 323, 468, 476, 1032
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 4 cut(s) 14, 140, 783, 1045
BfmI CTRYAG 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 883
BfuAI ACCTGC 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 3 cut(s) 5, 96, 800
BlsI GCNGC 3 cut(s) 6, 97, 801
Bme1390I CCNGG 2 cut(s) 362, 1030
BmeT110I CYCGRG 1 cut(s) 866
BmiI GGNNCC 3 cut(s) 366, 622, 922
BmrFI CCNGG 2 cut(s) 362, 1030
BmsI GCATC 1 cut(s) 960
BpiI GAAGAC 1 cut(s) 637
BpmI CTGGAG 1 cut(s) 294
BpuMI CCSGG 1 cut(s) 1030
Bsa29I ATCGAT 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 1021
BsaJI CCNNGG 1 cut(s) 1029
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 356
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 2 cut(s) 311, 460
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 1029
BseGI GGATG 1 cut(s) 800
BseJI GATNNNNATC 1 cut(s) 1021
BseLI CCNNNNNNNGG 2 cut(s) 225, 356
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 479, 654
BseNI ACTGG 2 cut(s) 311, 460
BseSI GKGCMC 1 cut(s) 882
BseXI GCAGC 3 cut(s) 16, 82, 811
BseYI CCCAGC 2 cut(s) 550, 616
BsgI GTGCAG 2 cut(s) 81, 399
BshFI GGCC 2 cut(s) 360, 897
BshTI ACCGGT 1 cut(s) 174
BshVI ATCGAT 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 882
BsiHKCI CYCGRG 1 cut(s) 866
BsiSI CCGG 2 cut(s) 175, 1029
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 225, 356
BsmAI GTCTC 4 cut(s) 323, 468, 476, 1032
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 2 cut(s) 360, 897
BsoBI CYCGRG 1 cut(s) 866
Bsp1286I GDGCHC 2 cut(s) 538, 882
Bsp1407I TGTACA 1 cut(s) 928
Bsp143I GATC 2 cut(s) 364, 1016
BspACI CCGC 2 cut(s) 924, 1035
BspANI GGCC 2 cut(s) 360, 897
BspCNI CTCAG 2 cut(s) 478, 653
BspDI ATCGAT 1 cut(s) 146
BspLI GGNNCC 3 cut(s) 366, 622, 922
BspMAI CTGCAG 1 cut(s) 126
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 2 cut(s) 359, 372
BspTI CTTAAG 1 cut(s) 883
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 174
BsrGI TGTACA 1 cut(s) 928
BsrI ACTGG 2 cut(s) 311, 460
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 1 cut(s) 1029
BssMI GATC 2 cut(s) 364, 1016
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 118, 444
Bst6I CTCTTC 2 cut(s) 518, 945
BstAFI CTTAAG 1 cut(s) 883
BstAUI TGTACA 1 cut(s) 928
BstC8I GCNNGC 2 cut(s) 231, 388
BstDEI CTNAG 2 cut(s) 465, 640
BstEII GGTNACC 1 cut(s) 170
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 1 cut(s) 800
BstKTI GATC 2 cut(s) 367, 1019
BstMAI GTCTC 4 cut(s) 323, 468, 476, 1032
BstMBI GATC 2 cut(s) 364, 1016
BstMWI GCNNNNNNNGC 3 cut(s) 239, 533, 989
BstNI CCWGG 1 cut(s) 362
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 2 cut(s) 360, 1028
BstSFI CTRYAG 1 cut(s) 122
BstSLI GKGCMC 1 cut(s) 882
BstV1I GCAGC 3 cut(s) 16, 82, 811
BstV2I GAAGAC 1 cut(s) 637
BstX2I RGATCY 1 cut(s) 364
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 1 cut(s) 364
Bsu15I ATCGAT 1 cut(s) 146
BsuRI GGCC 2 cut(s) 360, 897
BsuTUI ATCGAT 1 cut(s) 146
BtsCI GGATG 1 cut(s) 800
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 1 cut(s) 117
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 231, 388
Cfr10I RCCGGY 1 cut(s) 174
ClaI ATCGAT 1 cut(s) 146
Csp6I GTAC 4 cut(s) 119, 276, 863, 929
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 3 cut(s) 334, 531, 689
CviQI GTAC 4 cut(s) 119, 276, 863, 929
DdeI CTNAG 2 cut(s) 465, 640
DpnI GATC 2 cut(s) 366, 1018
DpnII GATC 2 cut(s) 364, 1016
DrdI GACNNNNNNGTC 1 cut(s) 168
DseDI GACNNNNNNGTC 1 cut(s) 168
Eam1104I CTCTTC 2 cut(s) 518, 945
EarI CTCTTC 2 cut(s) 518, 945
Eco147I AGGCCT 1 cut(s) 897
Eco24I GRGCYC 1 cut(s) 538
Eco32I GATATC 1 cut(s) 288
Eco57I CTGAAG 3 cut(s) 173, 365, 568
Eco88I CYCGRG 1 cut(s) 866
Eco91I GGTNACC 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO65I GGTNACC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 360
EcoRV GATATC 1 cut(s) 288
EcoT38I GRGCYC 1 cut(s) 538
FaeI CATG 3 cut(s) 337, 534, 692
FaqI GGGAC 1 cut(s) 36
FatI CATG 3 cut(s) 333, 530, 688
Fnu4HI GCNGC 3 cut(s) 5, 96, 800
FokI GGATG 1 cut(s) 807
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 3 cut(s) 5, 96, 800
FspBI CTAG 4 cut(s) 14, 140, 783, 1045
GluI GCNGC 3 cut(s) 5, 96, 800
GsaI CCCAGC 2 cut(s) 554, 620
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 360, 897
HapII CCGG 2 cut(s) 175, 1029
Hin1II CATG 3 cut(s) 337, 534, 692
HincII GTYRAC 1 cut(s) 447
HindII GTYRAC 1 cut(s) 447
HinfI GANTC 2 cut(s) 469, 677
HpaI GTTAAC 1 cut(s) 447
HpaII CCGG 2 cut(s) 175, 1029
HphI GGTGA 4 cut(s) 164, 173, 742, 946
Hpy166II GTNNAC 2 cut(s) 447, 880
Hpy188I TCNGA 2 cut(s) 161, 468
Hpy188III TCNNGA 2 cut(s) 195, 649
Hpy8I GTNNAC 2 cut(s) 447, 880
HpyAV CCTTC 5 cut(s) 73, 194, 389, 592, 767
HpyCH4III ACNGT 2 cut(s) 118, 444
HpyCH4V TGCA 9 cut(s) 4, 98, 110, 124, 416, 629, 738, 880, 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 533, 989
HpyF3I CTNAG 2 cut(s) 465, 640
Hsp92II CATG 3 cut(s) 337, 534, 692
KspAI GTTAAC 1 cut(s) 447
Kzo9I GATC 2 cut(s) 364, 1016
LmnI GCTCC 3 cut(s) 239, 492, 926
Lsp1109I GCAGC 3 cut(s) 16, 82, 811
LweI GCATC 1 cut(s) 960
MaeI CTAG 4 cut(s) 14, 140, 783, 1045
MaeIII GTNAC 3 cut(s) 112, 170, 748
MalI GATC 2 cut(s) 366, 1018
MboI GATC 2 cut(s) 364, 1016
MboII GAAGA 8 cut(s) 50, 88, 179, 500, 505, 637, 677, 932
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 2 cut(s) 538, 882
MluCI AATT 4 cut(s) 341, 432, 666, 967
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 3 cut(s) 139, 673, 823
MnlI CCTC 6 cut(s) 76, 318, 505, 538, 649, 779
MseI TTAA 3 cut(s) 438, 446, 884
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 926
MspCI CTTAAG 1 cut(s) 883
MspI CCGG 2 cut(s) 175, 1029
MspR9I CCNGG 2 cut(s) 362, 1030
MvaI CCWGG 1 cut(s) 362
MwoI GCNNNNNNNGC 3 cut(s) 239, 533, 989
NciI CCSGG 1 cut(s) 1030
NdeII GATC 2 cut(s) 364, 1016
NlaIII CATG 3 cut(s) 337, 534, 692
NlaIV GGNNCC 3 cut(s) 366, 622, 922
NmuCI GTSAC 3 cut(s) 112, 170, 748
PaqCI CACCTGC 1 cut(s) 194
PceI AGGCCT 1 cut(s) 897
PfeI GAWTC 1 cut(s) 677
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 3 cut(s) 6, 97, 801
PleI GAGTC 1 cut(s) 477
PpsI GAGTC 1 cut(s) 477
Psp6I CCWGG 1 cut(s) 360
PspEI GGTNACC 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 550, 616
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 366, 622, 922
PstI CTGCAG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 4 cut(s) 120, 277, 864, 930
RsaNI GTAC 4 cut(s) 119, 276, 863, 929
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 3 cut(s) 438, 446, 884
SatI GCNGC 3 cut(s) 5, 96, 800
Sau3AI GATC 2 cut(s) 364, 1016
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 2 cut(s) 362, 1030
SduI GDGCHC 2 cut(s) 538, 882
SetI ASST 9 cut(s) 97, 186, 208, 221, 352, 552, 571, 784, 959
SfaNI GCATC 1 cut(s) 960
SfcI CTRYAG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 1 cut(s) 883
SmoI CTYRAG 1 cut(s) 883
SpeI ACTAGT 1 cut(s) 139
Sse9I AATT 4 cut(s) 341, 432, 666, 967
SseBI AGGCCT 1 cut(s) 897
SsiI CCGC 2 cut(s) 924, 1035
SspI AATATT 1 cut(s) 395
SspMI CTAG 4 cut(s) 14, 140, 783, 1045
StuI AGGCCT 1 cut(s) 897
StyD4I CCNGG 2 cut(s) 360, 1028
TaaI ACNGT 2 cut(s) 118, 444
TaqI TCGA 2 cut(s) 146, 648
TasI AATT 4 cut(s) 341, 432, 666, 967
TatI WGTACW 2 cut(s) 275, 928
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 3 cut(s) 438, 446, 884
Tru9I TTAA 3 cut(s) 438, 446, 884
TscAI CASTG 1 cut(s) 117
TseFI GTSAC 3 cut(s) 112, 170, 748
TseI GCWGC 3 cut(s) 4, 95, 799
Tsp45I GTSAC 3 cut(s) 112, 170, 748
TspDTI ATGAA 3 cut(s) 51, 322, 677
TspRI CASTG 1 cut(s) 117
Vha464I CTTAAG 1 cut(s) 883
VneI GTGCAC 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 1 cut(s) 341
XspI CTAG 4 cut(s) 14, 140, 783, 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.