RLG00000014190

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003489
Physical Location & Seq
Forward (+)
48958218 .. 48962398
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014190

Sequence Viewer

Length: 804 bp
ATGAAAATCCTTGCTGATTCGTACCCATTCGAATTGCAATCGGAACAAGTCTGTATGACTGAAGTGAGCATCGCCAGAGAAAGAAGAAGACCTGATCCAAGAACTTGCTCTCCTTCACTGTCGCCCTACACGGCTTCATCTCCTCCATCGGACCCCCCACCGCCGCCCTGCCCTGTGGTACCGCATGGCAGTCTCGACCGCGTTGACTTGGGCATTGACCTCTGTGGCCTTCTTCCTTATCGAGGTCACCTTCTTCCTAGCAGGGCCGACTTGTATTCAAATGAGGGAGAGAAGCCTGATTGTATCATTACTCCGAAATTGAATGTTGGGGGTAAGCTTCGGGCTGATGCTAGCAATGGGAACACTAATGTATTCATGAATGGCAGAGAGATCACAAAGATTGAGCTTAGGGTGCTCAAGGTTGAAGATGAAGTGTCAAAGTTGGAGCAGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGCAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATATTCAAATGAAGCTATAGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTCTGACTGAGCTAGATCTTTTAGCAGAGGTGTATCCTTGTCCAAAAGGTTCGATAAGACACAAAGAATTGGTTAACAGATCTGGTGGAAAAGAGCAATTTCCTTTTCTCATTGATCCAAATACCGGTATTTCAATGTATGAAAGCGTATTTTATATTTGTTTGTTCAACTTCAACCTTTTGTTGAAAAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

268

Amino Acids

30.0

Weight (kDa)

6.47

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 140 - 201 2e-06 Microtubule associated protein (MAP65/ASE1 family)
GST_N_3 PF13417 192 - 251 1.6e-10 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 1 cut(s) 178
AccII CGCG 2 cut(s) 201, 603
AciI CCGC 4 cut(s) 161, 164, 182, 199
AclWI GGATC 3 cut(s) 89, 469, 716
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 23, 180
AfiI CCNNNNNNNGG 2 cut(s) 242, 731
AgeI ACCGGT 1 cut(s) 731
AgsI TTSAA 8 cut(s) 279, 322, 425, 561, 741, 775, 781, 793
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AloI GAACNNNNNNTCC 2 cut(s) 94, 126
AluBI AGCT 7 cut(s) 337, 406, 451, 466, 569, 608, 619
AluI AGCT 7 cut(s) 337, 406, 451, 466, 569, 608, 619
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 197, 525
AlwI GGATC 3 cut(s) 89, 469, 716
AoxI GGCC 3 cut(s) 226, 264, 576
ApeKI GCWGC 2 cut(s) 448, 548
AsiGI ACCGGT 1 cut(s) 731
Asp718I GGTACC 1 cut(s) 178
AspS9I GGNCC 2 cut(s) 151, 264
AsuHPI GGTGA 1 cut(s) 239
AsuII TTCGAA 1 cut(s) 30
AsuNHI GCTAGC 1 cut(s) 350
AvaII GGWCC 1 cut(s) 151
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 2 cut(s) 460, 535
BccI CCATC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 147
BciVI GTATCC 1 cut(s) 651
BcoDI GTCTC 2 cut(s) 197, 525
BfaI CTAG 3 cut(s) 258, 351, 620
BfmI CTRYAG 2 cut(s) 549, 570
BfuI GTATCC 1 cut(s) 651
BglII AGATCT 2 cut(s) 622, 686
BisI GCNGC 3 cut(s) 164, 449, 549
BlsI GCNGC 3 cut(s) 165, 450, 550
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 151, 264
BmiI GGNNCC 2 cut(s) 153, 180
BmsI GCATC 2 cut(s) 78, 337
BmtI GCTAGC 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 401, 437
BsaWI WCCGGW 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 731
Bse118I RCCGGY 1 cut(s) 731
Bse1I ACTGG 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 361
BseLI CCNNNNNNNGG 2 cut(s) 242, 731
BseMI GCAATG 1 cut(s) 361
BseMII CTCAG 2 cut(s) 533, 606
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 2 cut(s) 132, 518
BseXI GCAGC 2 cut(s) 460, 535
Bsh1236I CGCG 2 cut(s) 201, 603
Bsh1285I CGRYCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 228, 266, 578
BshNI GGYRCC 1 cut(s) 178
BshTI ACCGGT 1 cut(s) 731
BsiEI CGRYCG 1 cut(s) 199
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 1 cut(s) 732
BslI CCNNNNNNNGG 2 cut(s) 242, 731
BsmAI GTCTC 2 cut(s) 197, 525
BsnI GGCC 3 cut(s) 228, 266, 578
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 6 cut(s) 94, 390, 474, 622, 686, 721
Bsp68I TCGCGA 1 cut(s) 603
BspACI CCGC 4 cut(s) 161, 164, 182, 199
BspANI GGCC 3 cut(s) 228, 266, 578
BspCNI CTCAG 2 cut(s) 534, 607
BspFNI CGCG 2 cut(s) 201, 603
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 153, 180
BspMAI CTGCAG 1 cut(s) 553
BspOI GCTAGC 1 cut(s) 354
BspPI GGATC 3 cut(s) 89, 469, 716
BspT104I TTCGAA 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 178
BsrDI GCAATG 1 cut(s) 361
BsrFI RCCGGY 1 cut(s) 731
BsrI ACTGG 1 cut(s) 504
BssAI RCCGGY 1 cut(s) 731
BssMI GATC 6 cut(s) 94, 390, 474, 622, 686, 721
Bst4CI ACNGT 2 cut(s) 120, 519
BstBI TTCGAA 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 352, 580
BstDEI CTNAG 3 cut(s) 407, 542, 615
BstEII GGTNACC 1 cut(s) 245
BstENI CCTNNNNNAGG 1 cut(s) 240
BstFNI CGCG 2 cut(s) 201, 603
BstKTI GATC 6 cut(s) 97, 393, 477, 625, 689, 724
BstMAI GTCTC 2 cut(s) 197, 525
BstMBI GATC 6 cut(s) 94, 390, 474, 622, 686, 721
BstMCI CGRYCG 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 412, 575, 588
BstPI GGTNACC 1 cut(s) 245
BstSFI CTRYAG 2 cut(s) 549, 570
BstUI CGCG 2 cut(s) 201, 603
BstV1I GCAGC 2 cut(s) 460, 535
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 3 cut(s) 474, 622, 686
BstYI RGATCY 3 cut(s) 474, 622, 686
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 3 cut(s) 228, 266, 578
BtgZI GCGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 116
BtuMI TCGCGA 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 352, 580
CciI TCATGA 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 731
Cfr13I GGNCC 2 cut(s) 151, 264
Csp6I GTAC 2 cut(s) 22, 179
CspAI ACCGGT 1 cut(s) 731
CviAII CATG 3 cut(s) 185, 376, 526
CviQI GTAC 2 cut(s) 22, 179
DdeI CTNAG 3 cut(s) 407, 542, 615
DpnI GATC 6 cut(s) 96, 392, 476, 624, 688, 723
DpnII GATC 6 cut(s) 94, 390, 474, 622, 686, 721
Eco147I AGGCCT 1 cut(s) 578
Eco47I GGWCC 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 81
Eco91I GGTNACC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 245
FaeI CATG 3 cut(s) 188, 379, 529
FaiI YATR 8 cut(s) 56, 186, 377, 511, 527, 572, 747, 762
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FatI CATG 3 cut(s) 184, 375, 525
Fnu4HI GCNGC 3 cut(s) 164, 449, 549
Fsp4HI GCNGC 3 cut(s) 164, 449, 549
FspBI CTAG 3 cut(s) 258, 351, 620
GluI GCNGC 3 cut(s) 164, 449, 549
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 3 cut(s) 228, 266, 578
HapII CCGG 1 cut(s) 732
Hin1II CATG 3 cut(s) 188, 379, 529
HincII GTYRAC 2 cut(s) 205, 682
HindII GTYRAC 2 cut(s) 205, 682
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 1 cut(s) 17
HpaI GTTAAC 1 cut(s) 682
HpaII CCGG 1 cut(s) 732
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 205, 682
Hpy188I TCNGA 5 cut(s) 43, 151, 315, 483, 612
Hpy188III TCNNGA 3 cut(s) 194, 376, 602
Hpy8I GTNNAC 2 cut(s) 205, 682
HpyAV CCTTC 4 cut(s) 123, 239, 260, 488
HpyCH4III ACNGT 2 cut(s) 120, 519
HpyCH4V TGCA 4 cut(s) 37, 513, 551, 591
HpyF10VI GCNNNNNNNGC 3 cut(s) 412, 575, 588
HpyF3I CTNAG 3 cut(s) 407, 542, 615
Hsp92II CATG 3 cut(s) 188, 379, 529
KpnI GGTACC 1 cut(s) 182
KspAI GTTAAC 1 cut(s) 682
Kzo9I GATC 6 cut(s) 94, 390, 474, 622, 686, 721
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 2 cut(s) 460, 535
LweI GCATC 2 cut(s) 78, 337
MaeI CTAG 3 cut(s) 258, 351, 620
MaeIII GTNAC 2 cut(s) 245, 529
MalI GATC 6 cut(s) 96, 392, 476, 624, 688, 723
MboI GATC 6 cut(s) 94, 390, 474, 622, 686, 721
MboII GAAGA 5 cut(s) 96, 99, 224, 245, 437
MflI RGATCY 3 cut(s) 474, 622, 686
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 5 cut(s) 32, 317, 459, 674, 704
MmeI TCCRAC 2 cut(s) 423, 474
MnlI CCTC 9 cut(s) 153, 230, 236, 277, 496, 499, 568, 588, 628
MseI TTAA 2 cut(s) 462, 681
MspI CCGG 1 cut(s) 732
MvnI CGCG 2 cut(s) 201, 603
MwoI GCNNNNNNNGC 3 cut(s) 412, 575, 588
NdeII GATC 6 cut(s) 94, 390, 474, 622, 686, 721
NheI GCTAGC 1 cut(s) 350
NlaIII CATG 3 cut(s) 188, 379, 529
NlaIV GGNNCC 2 cut(s) 153, 180
NmuCI GTSAC 1 cut(s) 245
NruI TCGCGA 1 cut(s) 603
NspV TTCGAA 1 cut(s) 30
PagI TCATGA 1 cut(s) 375
PceI AGGCCT 1 cut(s) 578
PfeI GAWTC 1 cut(s) 17
PinAI ACCGGT 1 cut(s) 731
PkrI GCNGC 3 cut(s) 165, 450, 550
PspEI GGTNACC 1 cut(s) 245
PspN4I GGNNCC 2 cut(s) 153, 180
PspPI GGNCC 2 cut(s) 151, 264
PstI CTGCAG 1 cut(s) 553
PsuI RGATCY 3 cut(s) 474, 622, 686
RruI TCGCGA 1 cut(s) 603
RsaI GTAC 2 cut(s) 23, 180
RsaNI GTAC 2 cut(s) 22, 179
SaqAI TTAA 2 cut(s) 462, 681
SatI GCNGC 3 cut(s) 164, 449, 549
Sau3AI GATC 6 cut(s) 94, 390, 474, 622, 686, 721
Sau96I GGNCC 2 cut(s) 151, 264
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 2 cut(s) 78, 337
SfcI CTRYAG 2 cut(s) 549, 570
SfuI TTCGAA 1 cut(s) 30
SinI GGWCC 1 cut(s) 151
SmlI CTYRAG 2 cut(s) 416, 452
SmoI CTYRAG 2 cut(s) 416, 452
Sse9I AATT 5 cut(s) 32, 317, 459, 674, 704
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 4 cut(s) 161, 164, 182, 199
SspI AATATT 1 cut(s) 557
SspMI CTAG 3 cut(s) 258, 351, 620
StuI AGGCCT 1 cut(s) 578
TaaI ACNGT 2 cut(s) 120, 519
TaqI TCGA 4 cut(s) 30, 195, 241, 659
TasI AATT 5 cut(s) 32, 317, 459, 674, 704
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 2 cut(s) 462, 681
Tru9I TTAA 2 cut(s) 462, 681
TscAI CASTG 1 cut(s) 123
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 2 cut(s) 448, 548
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 7 cut(s) 17, 126, 364, 392, 444, 579, 762
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 151
XagI CCTNNNNNAGG 1 cut(s) 240
XspI CTAG 3 cut(s) 258, 351, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.