Rw1G011050

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0003489
Physical Location & Seq
Forward (+)
24973469 .. 24975678
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G011050.1

Sequence Viewer

Length: 366 bp
ATGAAGGTTGAAGATGAATTGTCAAAGTTGGAGCAGGTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGTAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATACTCAAATGAAGCTATAGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTCTGACTGAGCTAGATCTTTTAGCAGAGGTTCTTGAAGTTAGACGAGACTTGAAGATGACAACTAGAAAAATTGCATCTCAGTATGCTGCAACTGGCATGTATGCAGAACTGATGCAAAGGGTTCTTATGGATGCGTATCATTTGGGTAACCTCCAACCTTCAATCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

121

Amino Acids

13.94

Weight (kDa)

5.77

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 3 - 69 8.6e-06 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 189
AclWI GGATC 1 cut(s) 55
AgsI TTSAA 4 cut(s) 11, 230, 247, 357
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
AluBI AGCT 4 cut(s) 52, 155, 194, 205
AluI AGCT 4 cut(s) 52, 155, 194, 205
Alw26I GTCTC 2 cut(s) 111, 234
AlwI GGATC 1 cut(s) 55
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 134, 281
BbvI GCAGC 2 cut(s) 121, 268
BcoDI GTCTC 2 cut(s) 111, 234
BfaI CTAG 2 cut(s) 206, 258
BfmI CTRYAG 2 cut(s) 135, 156
BfuAI ACCTGC 1 cut(s) 25
BglII AGATCT 1 cut(s) 208
BisI GCNGC 2 cut(s) 135, 282
BlsI GCNGC 2 cut(s) 136, 283
BmsI GCATC 3 cut(s) 278, 297, 316
BpmI CTGGAG 1 cut(s) 107
BsaBI GATNNNNATC 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 90, 292
Bse8I GATNNNNATC 1 cut(s) 330
BseGI GGATG 1 cut(s) 331
BseJI GATNNNNATC 1 cut(s) 330
BseMII CTCAG 3 cut(s) 119, 192, 287
BseNI ACTGG 2 cut(s) 90, 292
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 2 cut(s) 121, 268
Bsh1236I CGCG 1 cut(s) 189
BshFI GGCC 1 cut(s) 164
BsmAI GTCTC 2 cut(s) 111, 234
BsnI GGCC 1 cut(s) 164
Bsp143I GATC 2 cut(s) 60, 208
Bsp68I TCGCGA 1 cut(s) 189
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 3 cut(s) 120, 193, 286
BspFNI CGCG 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 25
BspPI GGATC 1 cut(s) 55
BsrI ACTGG 2 cut(s) 90, 292
BssMI GATC 2 cut(s) 60, 208
Bst4CI ACNGT 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 3 cut(s) 128, 201, 273
BstEII GGTNACC 1 cut(s) 341
BstF5I GGATG 1 cut(s) 331
BstFNI CGCG 1 cut(s) 189
BstKTI GATC 2 cut(s) 63, 211
BstMAI GTCTC 2 cut(s) 111, 234
BstMBI GATC 2 cut(s) 60, 208
BstMWI GCNNNNNNNGC 2 cut(s) 161, 174
BstNSI RCATGY 1 cut(s) 295
BstPI GGTNACC 1 cut(s) 341
BstSFI CTRYAG 2 cut(s) 135, 156
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 121, 268
BstX2I RGATCY 2 cut(s) 60, 208
BstYI RGATCY 2 cut(s) 60, 208
BsuRI GGCC 1 cut(s) 164
BtsCI GGATG 1 cut(s) 331
BtuMI TCGCGA 1 cut(s) 189
BveI ACCTGC 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 166
CviAII CATG 2 cut(s) 112, 292
CviJI RGCY 5 cut(s) 52, 155, 164, 194, 205
CviKI_1 RGCY 5 cut(s) 52, 155, 164, 194, 205
DdeI CTNAG 3 cut(s) 128, 201, 273
DpnI GATC 2 cut(s) 62, 210
DpnII GATC 2 cut(s) 60, 208
Eco147I AGGCCT 1 cut(s) 164
Eco91I GGTNACC 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 341
FaeI CATG 2 cut(s) 115, 295
FaiI YATR 7 cut(s) 97, 113, 158, 279, 293, 297, 323
FalI AAGNNNNNCTT 2 cut(s) 48, 80
FatI CATG 2 cut(s) 111, 291
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 135, 282
FokI GGATG 1 cut(s) 338
Fsp4HI GCNGC 2 cut(s) 135, 282
FspBI CTAG 2 cut(s) 206, 258
GluI GCNGC 2 cut(s) 135, 282
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 1 cut(s) 164
Hin1II CATG 2 cut(s) 115, 295
Hpy166II GTNNAC 1 cut(s) 101
Hpy188I TCNGA 2 cut(s) 69, 198
Hpy188III TCNNGA 2 cut(s) 188, 227
Hpy8I GTNNAC 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 74, 363
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4V TGCA 6 cut(s) 137, 177, 269, 284, 299, 310
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 174
HpyF3I CTNAG 3 cut(s) 128, 201, 273
Hsp92II CATG 2 cut(s) 115, 295
Kzo9I GATC 2 cut(s) 60, 208
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 5 cut(s) 20, 71, 163, 178, 273
Lsp1109I GCAGC 2 cut(s) 121, 268
LweI GCATC 3 cut(s) 278, 297, 316
MaeI CTAG 2 cut(s) 206, 258
MaeIII GTNAC 2 cut(s) 115, 341
MalI GATC 2 cut(s) 62, 210
MboI GATC 2 cut(s) 60, 208
MboII GAAGA 2 cut(s) 23, 259
MflI RGATCY 2 cut(s) 60, 208
MluCI AATT 3 cut(s) 17, 45, 264
MmeI TCCRAC 2 cut(s) 9, 60
MnlI CCTC 6 cut(s) 82, 85, 154, 174, 214, 356
MseI TTAA 2 cut(s) 48, 364
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 2 cut(s) 161, 174
NdeII GATC 2 cut(s) 60, 208
NlaIII CATG 2 cut(s) 115, 295
NruI TCGCGA 1 cut(s) 189
NspI RCATGY 1 cut(s) 295
PceI AGGCCT 1 cut(s) 164
PkrI GCNGC 2 cut(s) 136, 283
PspEI GGTNACC 1 cut(s) 341
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 2 cut(s) 60, 208
RruI TCGCGA 1 cut(s) 189
SaqAI TTAA 2 cut(s) 48, 364
SatI GCNGC 2 cut(s) 135, 282
Sau3AI GATC 2 cut(s) 60, 208
SfaNI GCATC 3 cut(s) 278, 297, 316
SfcI CTRYAG 2 cut(s) 135, 156
Sse9I AATT 3 cut(s) 17, 45, 264
SseBI AGGCCT 1 cut(s) 164
SspMI CTAG 2 cut(s) 206, 258
StuI AGGCCT 1 cut(s) 164
TaaI ACNGT 1 cut(s) 105
TasI AATT 3 cut(s) 17, 45, 264
Tru1I TTAA 2 cut(s) 48, 364
Tru9I TTAA 2 cut(s) 48, 364
TseI GCWGC 2 cut(s) 134, 281
TspDTI ATGAA 3 cut(s) 17, 30, 165
XceI RCATGY 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 2 cut(s) 206, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.