RLG00000019050

Extra-large guanine nucleotide-binding protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003489
Physical Location & Seq
Reverse (-)
45567255 .. 45577502
10248 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019050

Sequence Viewer

Length: 417 bp
ATGTGTTCGATCGGTATACCGACCCATTCTTCCGGGTCGGATCAGTTGGAGGGAGAGAAGCCTGATTGTATCATTACTCCGAAATTGAATGTTGGGGGTAAGCTTCGGGCTGATGCTAGCAATGGGAACACTAATGTATTCATGAATGGCAGAGAGATCACAAAGATTGAGCTTAGGGTGCTCAAGGTTGAAGATGAAGTGTCAAAGTTGGAGCAGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGCAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATACTCAAATGAAGCTATAGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTCTGACTGAGCTAGATCTTTTAGCAGAGGTGAGTAACATTTGA

Protein Analysis

139

Amino Acids

15.35

Weight (kDa)

5.33

Isoelectric Point (pI)

37.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 62 - 123 4.1e-07 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 16
AccII CGCG 1 cut(s) 369
AclWI GGATC 2 cut(s) 48, 235
AgsI TTSAA 2 cut(s) 88, 191
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 7 cut(s) 103, 172, 217, 232, 335, 374, 385
AluI AGCT 7 cut(s) 103, 172, 217, 232, 335, 374, 385
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 291
AlwI GGATC 2 cut(s) 48, 235
AoxI GGCC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 214, 314
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 415
AsuNHI GCTAGC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 2 cut(s) 226, 301
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 2 cut(s) 117, 386
BfmI CTRYAG 2 cut(s) 315, 336
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 215, 315
BlsI GCNGC 2 cut(s) 216, 316
Bme1390I CCNGG 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 103
BmtI GCTAGC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 167, 203
BpuMI CCSGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 2 cut(s) 299, 372
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 2 cut(s) 226, 301
Bsh1236I CGCG 1 cut(s) 369
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 33
BsmAI GTCTC 1 cut(s) 291
BsnI GGCC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 5 cut(s) 9, 40, 156, 240, 388
Bsp68I TCGCGA 1 cut(s) 369
BspANI GGCC 1 cut(s) 344
BspCNI CTCAG 2 cut(s) 300, 373
BspFNI CGCG 1 cut(s) 369
BspHI TCATGA 1 cut(s) 141
BspMAI CTGCAG 1 cut(s) 319
BspOI GCTAGC 1 cut(s) 120
BspPI GGATC 2 cut(s) 48, 235
BsrDI GCAATG 1 cut(s) 127
BsrI ACTGG 1 cut(s) 270
BssMI GATC 5 cut(s) 9, 40, 156, 240, 388
BssNAI GTATAC 1 cut(s) 17
Bst1107I GTATAC 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 285
BstC8I GCNNGC 2 cut(s) 118, 346
BstDEI CTNAG 3 cut(s) 173, 308, 381
BstFNI CGCG 1 cut(s) 369
BstKTI GATC 5 cut(s) 12, 43, 159, 243, 391
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 5 cut(s) 9, 40, 156, 240, 388
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 3 cut(s) 178, 341, 354
BstSCI CCNGG 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 315, 336
BstUI CGCG 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 226, 301
BstX2I RGATCY 2 cut(s) 240, 388
BstYI RGATCY 2 cut(s) 240, 388
BstZ17I GTATAC 1 cut(s) 17
BsuRI GGCC 1 cut(s) 344
BtuMI TCGCGA 1 cut(s) 369
Cac8I GCNNGC 2 cut(s) 118, 346
CciI TCATGA 1 cut(s) 141
CviAII CATG 2 cut(s) 142, 292
DdeI CTNAG 3 cut(s) 173, 308, 381
DpnI GATC 5 cut(s) 11, 42, 158, 242, 390
DpnII GATC 5 cut(s) 9, 40, 156, 240, 388
Eco147I AGGCCT 1 cut(s) 344
FaeI CATG 2 cut(s) 145, 295
FaiI YATR 5 cut(s) 17, 143, 277, 293, 338
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FatI CATG 2 cut(s) 141, 291
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 215, 315
Fsp4HI GCNGC 2 cut(s) 215, 315
FspBI CTAG 2 cut(s) 117, 386
GluI GCNGC 2 cut(s) 215, 315
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 1 cut(s) 344
HapII CCGG 1 cut(s) 33
Hin1II CATG 2 cut(s) 145, 295
HindIII AAGCTT 1 cut(s) 101
HpaII CCGG 1 cut(s) 33
HphI GGTGA 1 cut(s) 415
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 4 cut(s) 40, 81, 249, 378
Hpy188III TCNNGA 2 cut(s) 142, 368
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 279, 317, 357
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 341, 354
HpyF3I CTNAG 3 cut(s) 173, 308, 381
Hsp92II CATG 2 cut(s) 145, 295
Kzo9I GATC 5 cut(s) 9, 40, 156, 240, 388
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 5 cut(s) 46, 75, 251, 343, 358
Lsp1109I GCAGC 2 cut(s) 226, 301
LweI GCATC 1 cut(s) 103
MaeI CTAG 2 cut(s) 117, 386
MaeIII GTNAC 2 cut(s) 295, 407
MalI GATC 5 cut(s) 11, 42, 158, 242, 390
MboI GATC 5 cut(s) 9, 40, 156, 240, 388
MboII GAAGA 2 cut(s) 21, 203
MflI RGATCY 2 cut(s) 240, 388
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 2 cut(s) 83, 225
MmeI TCCRAC 4 cut(s) 18, 27, 189, 240
MnlI CCTC 6 cut(s) 43, 262, 265, 334, 354, 394
MseI TTAA 1 cut(s) 228
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 178, 341, 354
NciI CCSGG 1 cut(s) 34
NdeII GATC 5 cut(s) 9, 40, 156, 240, 388
NheI GCTAGC 1 cut(s) 116
NlaIII CATG 2 cut(s) 145, 295
NruI TCGCGA 1 cut(s) 369
PagI TCATGA 1 cut(s) 141
PceI AGGCCT 1 cut(s) 344
PkrI GCNGC 2 cut(s) 216, 316
Ple19I CGATCG 1 cut(s) 12
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 2 cut(s) 240, 388
PvuI CGATCG 1 cut(s) 12
RruI TCGCGA 1 cut(s) 369
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 215, 315
Sau3AI GATC 5 cut(s) 9, 40, 156, 240, 388
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 1 cut(s) 103
SfcI CTRYAG 2 cut(s) 315, 336
SmlI CTYRAG 2 cut(s) 182, 218
SmoI CTYRAG 2 cut(s) 182, 218
Sse9I AATT 2 cut(s) 83, 225
SseBI AGGCCT 1 cut(s) 344
SspMI CTAG 2 cut(s) 117, 386
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 8
TasI AATT 2 cut(s) 83, 225
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 2 cut(s) 214, 314
TspDTI ATGAA 4 cut(s) 130, 158, 210, 345
XmiI GTMKAC 1 cut(s) 16
XspI CTAG 2 cut(s) 117, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.