Rw2G052930

Extra-large guanine nucleotide-binding protein 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003489
Physical Location & Seq
Forward (+)
80827144 .. 80828676
1533 bp
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UTR
Exon/CDS
Intron
Rw2G052930.1

Sequence Viewer

Length: 624 bp
ATGATAGATTTTACTTCGTATAGGTTGGGTACTGTTCTTTTGTGGATTTGTGTTTCGTTGTGTTTGTGTATTTTATTTCAGGAGGGAGAGAAGCCTGATTGTATCATTACTCCGAAGCTGAATGTTGGGGGTAAGATTCGGGCTGATGCCAGCAATGGGAACACTAATGTATTCATGAATGGCAGAGAGATCACAAAGATTGAGCTTAGGGTGCGGGGTGCTCAAGCTGGCCAAAGTACAGTGCCCTCCAGATACTCATTTATGGGTGTTGAAGATGAATTGTCAAAGTTGGAGCAGGTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGTAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATACTCAAATGAAGCTATAGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTCTGACTGAGCTAGATCTTTTAGCAGAGGTTCTTGTAGTTGGACGAGACTTGAAGATGACAACTAGAAAAATTGCATCTCAGTATGCTGCAACTGGCATGTATGCAGAACTGATGCAAAGGGTTCTTATGGATGCGTATCATTTGGGTAACCTCCAACCTTCAATGTAG

Protein Analysis

207

Amino Acids

23.26

Weight (kDa)

6.74

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 86 - 150 3.6e-06 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 361
AccII CGCG 1 cut(s) 450
AciI CCGC 1 cut(s) 214
AclWI GGATC 1 cut(s) 316
AcoI YGGCCR 1 cut(s) 229
AfaI GTAC 2 cut(s) 31, 238
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 3 cut(s) 272, 508, 618
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 7 cut(s) 118, 205, 227, 313, 416, 455, 466
AluI AGCT 7 cut(s) 118, 205, 227, 313, 416, 455, 466
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 2 cut(s) 372, 495
AlwI GGATC 1 cut(s) 316
AoxI GGCC 2 cut(s) 229, 423
ApeKI GCWGC 2 cut(s) 395, 542
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 382, 529
BcoDI GTCTC 2 cut(s) 372, 495
BfaI CTAG 2 cut(s) 467, 519
BfmI CTRYAG 2 cut(s) 396, 417
BfuAI ACCTGC 1 cut(s) 286
BglII AGATCT 1 cut(s) 469
BisI GCNGC 2 cut(s) 396, 543
BlsI GCNGC 2 cut(s) 397, 544
BmsI GCATC 4 cut(s) 136, 539, 558, 577
BpmI CTGGAG 2 cut(s) 232, 368
Bpu10I CCTNAGC 1 cut(s) 206
BpuEI CTTGAG 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 591
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 351, 553
Bse3DI GCAATG 1 cut(s) 160
Bse8I GATNNNNATC 1 cut(s) 591
BseGI GGATG 1 cut(s) 592
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 160
BseMII CTCAG 3 cut(s) 380, 453, 548
BseNI ACTGG 2 cut(s) 351, 553
BseRI GAGGAG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 2 cut(s) 382, 529
Bsh1236I CGCG 1 cut(s) 450
BshFI GGCC 2 cut(s) 231, 425
BsiHKAI GWGCWC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 372, 495
BsnI GGCC 2 cut(s) 231, 425
Bsp1286I GDGCHC 2 cut(s) 223, 246
Bsp143I GATC 3 cut(s) 189, 321, 469
Bsp68I TCGCGA 1 cut(s) 450
BspACI CCGC 1 cut(s) 214
BspANI GGCC 2 cut(s) 231, 425
BspCNI CTCAG 3 cut(s) 381, 454, 547
BspFNI CGCG 1 cut(s) 450
BspHI TCATGA 1 cut(s) 174
BspMAI CTGCAG 1 cut(s) 400
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 160
BsrI ACTGG 2 cut(s) 351, 553
BssMI GATC 3 cut(s) 189, 321, 469
Bst4CI ACNGT 3 cut(s) 34, 241, 366
BstC8I GCNNGC 3 cut(s) 151, 229, 427
BstDEI CTNAG 4 cut(s) 206, 389, 462, 534
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 1 cut(s) 592
BstFNI CGCG 1 cut(s) 450
BstKTI GATC 3 cut(s) 192, 324, 472
BstMAI GTCTC 2 cut(s) 372, 495
BstMBI GATC 3 cut(s) 189, 321, 469
BstMWI GCNNNNNNNGC 3 cut(s) 211, 422, 435
BstNSI RCATGY 1 cut(s) 556
BstPI GGTNACC 1 cut(s) 602
BstSFI CTRYAG 2 cut(s) 396, 417
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 2 cut(s) 382, 529
BstX2I RGATCY 2 cut(s) 321, 469
BstYI RGATCY 2 cut(s) 321, 469
BsuRI GGCC 2 cut(s) 231, 425
BtsCI GGATG 1 cut(s) 592
BtsIMutI CAGTG 1 cut(s) 246
BtuMI TCGCGA 1 cut(s) 450
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 3 cut(s) 151, 229, 427
CciI TCATGA 1 cut(s) 174
Csp6I GTAC 2 cut(s) 30, 237
CviAII CATG 3 cut(s) 175, 373, 553
CviQI GTAC 2 cut(s) 30, 237
DdeI CTNAG 4 cut(s) 206, 389, 462, 534
DpnI GATC 3 cut(s) 191, 323, 471
DpnII GATC 3 cut(s) 189, 321, 469
EaeI YGGCCR 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 425
Eco91I GGTNACC 1 cut(s) 602
EcoO65I GGTNACC 1 cut(s) 602
FaeI CATG 3 cut(s) 178, 376, 556
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 3 cut(s) 174, 372, 552
FauI CCCGC 1 cut(s) 207
FblI GTMKAC 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 396, 543
FokI GGATG 1 cut(s) 599
Fsp4HI GCNGC 2 cut(s) 396, 543
FspBI CTAG 2 cut(s) 467, 519
GluI GCNGC 2 cut(s) 396, 543
GsuI CTGGAG 2 cut(s) 232, 368
HaeIII GGCC 2 cut(s) 231, 425
Hin1II CATG 3 cut(s) 178, 376, 556
HinfI GANTC 1 cut(s) 136
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 3 cut(s) 114, 330, 459
Hpy188III TCNNGA 4 cut(s) 80, 175, 249, 449
Hpy8I GTNNAC 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 335, 624
HpyCH4III ACNGT 3 cut(s) 34, 241, 366
HpyCH4V TGCA 6 cut(s) 398, 438, 530, 545, 560, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 422, 435
HpyF3I CTNAG 4 cut(s) 206, 389, 462, 534
Hsp92II CATG 3 cut(s) 178, 376, 556
Kzo9I GATC 3 cut(s) 189, 321, 469
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 2 cut(s) 382, 529
LweI GCATC 4 cut(s) 136, 539, 558, 577
MaeI CTAG 2 cut(s) 467, 519
MaeIII GTNAC 2 cut(s) 376, 602
MalI GATC 3 cut(s) 191, 323, 471
MboI GATC 3 cut(s) 189, 321, 469
MboII GAAGA 2 cut(s) 284, 520
MflI RGATCY 2 cut(s) 321, 469
MhlI GDGCHC 2 cut(s) 223, 246
MlsI TGGCCA 1 cut(s) 231
MluCI AATT 3 cut(s) 278, 306, 525
MluNI TGGCCA 1 cut(s) 231
MmeI TCCRAC 3 cut(s) 270, 321, 475
MnlI CCTC 8 cut(s) 76, 256, 343, 346, 415, 435, 475, 617
Mox20I TGGCCA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 231
MseI TTAA 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 231
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 3 cut(s) 211, 422, 435
NdeII GATC 3 cut(s) 189, 321, 469
NlaIII CATG 3 cut(s) 178, 376, 556
NruI TCGCGA 1 cut(s) 450
NspI RCATGY 1 cut(s) 556
PagI TCATGA 1 cut(s) 174
PceI AGGCCT 1 cut(s) 425
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 397, 544
PspEI GGTNACC 1 cut(s) 602
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 2 cut(s) 321, 469
RruI TCGCGA 1 cut(s) 450
RsaI GTAC 2 cut(s) 31, 238
RsaNI GTAC 2 cut(s) 30, 237
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 396, 543
Sau3AI GATC 3 cut(s) 189, 321, 469
SduI GDGCHC 2 cut(s) 223, 246
SfaNI GCATC 4 cut(s) 136, 539, 558, 577
SfcI CTRYAG 2 cut(s) 396, 417
SmlI CTYRAG 1 cut(s) 222
SmoI CTYRAG 1 cut(s) 222
Sse9I AATT 3 cut(s) 278, 306, 525
SseBI AGGCCT 1 cut(s) 425
SsiI CCGC 1 cut(s) 214
SspMI CTAG 2 cut(s) 467, 519
StuI AGGCCT 1 cut(s) 425
TaaI ACNGT 3 cut(s) 34, 241, 366
TasI AATT 3 cut(s) 278, 306, 525
TatI WGTACW 1 cut(s) 236
TfiI GAWTC 1 cut(s) 136
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 1 cut(s) 246
TseI GCWGC 2 cut(s) 395, 542
TspDTI ATGAA 4 cut(s) 163, 191, 291, 426
TspRI CASTG 1 cut(s) 246
XceI RCATGY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 361
XspI CTAG 2 cut(s) 467, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.