Rorug06G0069500

peptidyl-tRNA hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003489
Physical Location & Seq
Forward (+)
9261173 .. 9261553
381 bp
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UTR
Exon/CDS
Intron
Rorug06G0069500.1

Sequence Viewer

Length: 381 bp
ATGATGGGGAAGTTTTTGCTCGTACTGGACGATATGTGGAATGAGAGTCCAAGTGATTGGGATGCCATGAAATTTCTTTTTAGCTTTGCAGCACCTGGGAGTAGGGTTTCGGTAACTGCTCGAAGTAAGACTATTTCATCCATTGTTACCACTAATGGTGATCTTACTTGTTTTTCGCCAACTACTTTGTCTAAGGAGGATTGTTGGGAAATTATCAAGAAAAAACTGAAAACAGAAGTAGATAATCGTGAAGATTTGAAAGCTATTGGCTTACAGTTAGCAGAGAAGTGTAAAGGCCTTCCTTTGGCGGCCACAATGATTAGAGATGCCTTGCATCTCAAATCAAAAGAGGTAGAGTGGGATGGGCTGCTGAAACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PTH2

Protein Analysis

126

Amino Acids

14.11

Weight (kDa)

6.58

Isoelectric Point (pI)

28.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 4 - 76 7e-12 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 1 cut(s) 71
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 259
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 2 cut(s) 84, 263
AluI AGCT 2 cut(s) 84, 263
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 2 cut(s) 295, 309
ApeKI GCWGC 2 cut(s) 89, 367
ApoI RAATTY 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 170
BbvI GCAGC 2 cut(s) 101, 354
BccI CCATC 1 cut(s) 356
BciT130I CCWGG 1 cut(s) 96
BisI GCNGC 3 cut(s) 90, 309, 368
BlsI GCNGC 3 cut(s) 91, 310, 369
Bme1390I CCNGG 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 52, 316, 343
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 3 cut(s) 67, 137, 367
BseLI CCNNNNNNNGG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 30
BseXI GCAGC 2 cut(s) 101, 354
BshFI GGCC 2 cut(s) 297, 311
BslI CCNNNNNNNGG 1 cut(s) 304
BsnI GGCC 2 cut(s) 297, 311
Bsp143I GATC 1 cut(s) 160
BspACI CCGC 1 cut(s) 308
BspANI GGCC 2 cut(s) 297, 311
BsrI ACTGG 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 1 cut(s) 160
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 276
BstDEI CTNAG 1 cut(s) 192
BstF5I GGATG 3 cut(s) 67, 137, 367
BstKTI GATC 1 cut(s) 163
BstMBI GATC 1 cut(s) 160
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 101, 354
BstXI CCANNNNNNTGG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 297, 311
BtsCI GGATG 3 cut(s) 67, 137, 367
CaiI CAGNNNCTG 1 cut(s) 95
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 67
CviJI RGCY 6 cut(s) 84, 263, 270, 297, 311, 367
CviKI_1 RGCY 6 cut(s) 84, 263, 270, 297, 311, 367
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 1 cut(s) 192
DpnI GATC 1 cut(s) 162
DpnII GATC 1 cut(s) 160
EaeI YGGCCR 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 94
FaeI CATG 1 cut(s) 70
FaiI YATR 2 cut(s) 35, 68
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 90, 309, 368
FokI GGATG 3 cut(s) 74, 124, 374
Fsp4HI GCNGC 3 cut(s) 90, 309, 368
GluI GCNGC 3 cut(s) 90, 309, 368
HaeIII GGCC 2 cut(s) 297, 311
Hin1II CATG 1 cut(s) 70
HinfI GANTC 1 cut(s) 46
HphI GGTGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 217, 248
HpyAV CCTTC 1 cut(s) 308
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4V TGCA 2 cut(s) 89, 334
HpyF3I CTNAG 1 cut(s) 192
Hsp92II CATG 1 cut(s) 70
Kzo9I GATC 1 cut(s) 160
LpnPI CCDG 3 cut(s) 11, 81, 108
Lsp1109I GCAGC 2 cut(s) 101, 354
LweI GCATC 3 cut(s) 52, 316, 343
MaeIII GTNAC 2 cut(s) 112, 145
MalI GATC 1 cut(s) 162
MboI GATC 1 cut(s) 160
MboII GAAGA 1 cut(s) 263
MluCI AATT 2 cut(s) 71, 210
MlyI GAGTC 1 cut(s) 55
MnlI CCTC 2 cut(s) 190, 343
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
NdeII GATC 1 cut(s) 160
NlaIII CATG 1 cut(s) 70
PceI AGGCCT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PkrI GCNGC 3 cut(s) 91, 310, 369
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SatI GCNGC 3 cut(s) 90, 309, 368
Sau3AI GATC 1 cut(s) 160
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 1 cut(s) 96
SetI ASST 4 cut(s) 86, 97, 265, 354
SfaNI GCATC 3 cut(s) 52, 316, 343
Sse9I AATT 2 cut(s) 71, 210
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 1 cut(s) 308
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 121
TasI AATT 2 cut(s) 71, 210
TauI GCSGC 1 cut(s) 311
TseI GCWGC 2 cut(s) 89, 367
TspDTI ATGAA 2 cut(s) 83, 126
XapI RAATTY 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.