RLG00000017164

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005208
Physical Location & Seq
Reverse (-)
13588634 .. 13593040
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017164

Sequence Viewer

Length: 945 bp
ATGTCCAAACATGACGTAGAACTTGGAAAAGTCCTCAAAATTGGAAACATGAACAATCAACTGCTCGTAGCTCCTGGAGGACCTGGACCCCTGGTTAGCAATCTGTCTCATCAACTAGTTAATCGGAGGAAAGCGATATACGCCCGCAACCTGAATCCAGGAAAGTCCATTTCTGGCATCGGCAGTCTGAGAAGCAAACAAACATGGTGGCCAAACGACCTCTGTATTGTGCAAATTAAGACATTGGAAATAACGTCCGTTGCCCTTCCGGCTCTGGATTCCCAAGACTCTGAGCACGCTAGCTGGTGCTATTCCCGGATCGTCGTCTCCGGTGACGCAATTGAAGAGGAAGCGGTCGGCGACACTTTGCATTCCGATTGTATCGACGAGATTTGGGGCTGCTCTGGGGACGCCGTCTACGGCGGCGTGGAGAGAGGTGGTGGAAGATTGAGAAGGTTGCTTCTCCGTATATTGTTTAAGGGGAAAAGAGTTGTTTTCCTTAAGCAGCTTTCATCTGGGTTGCTTCTTGTTACTGTCCTATGTGATTGGCCTTCCACCAAGGTCGATATTTCTGGGGTTAATGTGGAAAAATTTGATGACAAGTATTTCGGAAAGGCCGTGGAAAAGAAGAAGAAGAAGGGGGAGGGAGAGTTTTTTAAGGCAGAGAAAGAGGACAAGAAAGTGTTCCCACAAGAGAGGAAAGATGATCAGAAGAGTGTGGATGCTTCGTTGATAAAGTCCATTGACGTTGTCCCAGACTTGAAGACATACTTAGCTGCAAGATTCTCTCTGAGGTCTGGCATGTCTTTTAGACAGTCAGCTCTTTTCTTACTTAGGATACTTGTTCCCGTTCTTGTTTGTTTCTCTACTATGATTGTATGTTCTGTTCACATTGAGATCCAAGATTTTGTACTGATCGGCATTGCCTTGCAAGGGATGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

315

Amino Acids

34.73

Weight (kDa)

8.96

Isoelectric Point (pI)

40.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 185 - 270 4.9e-23 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 417
AciI CCGC 3 cut(s) 145, 353, 423
AclWI GGATC 2 cut(s) 326, 892
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 1 cut(s) 590
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 1 cut(s) 912
AfiI CCNNNNNNNGG 1 cut(s) 933
AflII CTTAAG 1 cut(s) 500
AgsI TTSAA 2 cut(s) 344, 763
AhlI ACTAGT 1 cut(s) 115
AjnI CCWGG 4 cut(s) 73, 82, 90, 157
AluBI AGCT 5 cut(s) 71, 303, 508, 776, 821
AluI AGCT 5 cut(s) 71, 303, 508, 776, 821
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 111, 331
AlwI GGATC 2 cut(s) 326, 892
AoxI GGCC 3 cut(s) 209, 548, 615
ApeKI GCWGC 3 cut(s) 399, 505, 776
ApoI RAATTY 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 590, 622
Asp700I GAANNNNTTC 1 cut(s) 683
AspS9I GGNCC 2 cut(s) 80, 86
AsuC2I CCSGG 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 344
AsuNHI GCTAGC 1 cut(s) 299
AvaII GGWCC 2 cut(s) 80, 86
BalI TGGCCA 1 cut(s) 211
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 386, 517, 763
BceAI ACGGC 3 cut(s) 398, 436, 602
BciT130I CCWGG 4 cut(s) 75, 84, 92, 159
BciVI GTATCC 1 cut(s) 831
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 1 cut(s) 316
BcoDI GTCTC 2 cut(s) 111, 331
BcuI ACTAGT 1 cut(s) 115
BfaI CTAG 2 cut(s) 116, 300
BfrI CTTAAG 1 cut(s) 500
BfuI GTATCC 1 cut(s) 831
BglI GCCNNNNNGGC 1 cut(s) 269
BisI GCNGC 4 cut(s) 400, 424, 506, 777
BlsI GCNGC 4 cut(s) 401, 425, 507, 778
Bme1390I CCNGG 5 cut(s) 75, 84, 92, 159, 316
Bme18I GGWCC 2 cut(s) 80, 86
BmgT120I GGNCC 2 cut(s) 80, 86
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 5 cut(s) 75, 84, 92, 159, 316
BmsI GCATC 2 cut(s) 186, 712
BmtI GCTAGC 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 770
BpmI CTGGAG 1 cut(s) 96
BpuMI CCSGG 1 cut(s) 316
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 90, 558, 618
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 1 cut(s) 933
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 4 cut(s) 75, 84, 92, 159
BseDI CCNNGG 3 cut(s) 90, 558, 618
BseGI GGATG 2 cut(s) 727, 942
BseLI CCNNNNNNNGG 1 cut(s) 933
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 3 cut(s) 179, 282, 782
BseXI GCAGC 3 cut(s) 386, 517, 763
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 211, 550, 617
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 269, 316, 330
BslFI GGGAC 2 cut(s) 422, 737
BslI CCNNNNNNNGG 1 cut(s) 933
BsmAI GTCTC 2 cut(s) 111, 331
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 2 cut(s) 422, 737
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 3 cut(s) 211, 550, 617
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 318, 706, 897, 915
BspACI CCGC 3 cut(s) 145, 353, 423
BspANI GGCC 3 cut(s) 211, 550, 617
BspCNI CTCAG 3 cut(s) 180, 283, 783
BspLI GGNNCC 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 303
BspPI GGATC 2 cut(s) 326, 892
BspTI CTTAAG 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 921
BssECI CCNNGG 3 cut(s) 90, 558, 618
BssMI GATC 4 cut(s) 318, 706, 897, 915
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 558
Bst2UI CCWGG 4 cut(s) 75, 84, 92, 159
Bst4CI ACNGT 2 cut(s) 535, 816
Bst6I CTCTTC 2 cut(s) 339, 707
BstACI GRCGYC 1 cut(s) 411
BstAFI CTTAAG 1 cut(s) 500
BstC8I GCNNGC 3 cut(s) 145, 297, 301
BstDEI CTNAG 5 cut(s) 188, 291, 772, 791, 833
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 727, 942
BstKTI GATC 4 cut(s) 321, 709, 900, 918
BstMAI GTCTC 2 cut(s) 111, 331
BstMBI GATC 4 cut(s) 318, 706, 897, 915
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 2 cut(s) 140, 269
BstNI CCWGG 4 cut(s) 75, 84, 92, 159
BstNSI RCATGY 1 cut(s) 805
BstSCI CCNGG 5 cut(s) 73, 82, 90, 157, 314
BstV1I GCAGC 3 cut(s) 386, 517, 763
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 1 cut(s) 897
BstYI RGATCY 1 cut(s) 897
BsuI GTATCC 1 cut(s) 831
BsuRI GGCC 3 cut(s) 211, 550, 617
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 2 cut(s) 727, 942
Cac8I GCNNGC 3 cut(s) 145, 297, 301
Cfr13I GGNCC 2 cut(s) 80, 86
CseI GACGC 2 cut(s) 344, 419
Csp6I GTAC 1 cut(s) 911
CspCI CAANNNNNGTGG 2 cut(s) 188, 223
CviAII CATG 4 cut(s) 11, 49, 204, 802
CviQI GTAC 1 cut(s) 911
DdeI CTNAG 5 cut(s) 188, 291, 772, 791, 833
DpnI GATC 4 cut(s) 320, 708, 899, 917
DpnII GATC 4 cut(s) 318, 706, 897, 915
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 2 cut(s) 339, 707
EarI CTCTTC 2 cut(s) 339, 707
Eco130I CCWWGG 1 cut(s) 558
Eco47I GGWCC 2 cut(s) 80, 86
EcoO109I RGGNCCY 1 cut(s) 80
EcoRII CCWGG 4 cut(s) 73, 82, 90, 157
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 4 cut(s) 14, 52, 207, 805
FalI AAGNNNNNCTT 2 cut(s) 755, 787
FaqI GGGAC 2 cut(s) 422, 737
FatI CATG 4 cut(s) 10, 48, 203, 801
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 4 cut(s) 400, 424, 506, 777
FokI GGATG 1 cut(s) 734
Fsp4HI GCNGC 4 cut(s) 400, 424, 506, 777
FspBI CTAG 2 cut(s) 116, 300
GluI GCNGC 4 cut(s) 400, 424, 506, 777
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 211, 550, 617
HapII CCGG 3 cut(s) 269, 316, 330
HgaI GACGC 2 cut(s) 344, 419
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 4 cut(s) 14, 52, 207, 805
HinfI GANTC 4 cut(s) 154, 278, 287, 783
HpaII CCGG 3 cut(s) 269, 316, 330
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 2 cut(s) 418, 889
Hpy188I TCNGA 7 cut(s) 126, 189, 292, 376, 611, 711, 792
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 2 cut(s) 418, 889
Hpy99I CGWCG 2 cut(s) 326, 389
HpyAV CCTTC 4 cut(s) 275, 447, 561, 631
HpyCH4III ACNGT 2 cut(s) 535, 816
HpyCH4IV ACGT 3 cut(s) 15, 254, 747
HpyCH4V TGCA 4 cut(s) 232, 370, 779, 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 269
HpyF3I CTNAG 5 cut(s) 188, 291, 772, 791, 833
HpySE526I ACGT 3 cut(s) 15, 254, 747
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 4 cut(s) 14, 52, 207, 805
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 4 cut(s) 318, 706, 897, 915
LmnI GCTCC 1 cut(s) 76
Lsp1109I GCAGC 3 cut(s) 386, 517, 763
LweI GCATC 2 cut(s) 186, 712
MaeI CTAG 2 cut(s) 116, 300
MaeII ACGT 3 cut(s) 15, 254, 747
MaeIII GTNAC 2 cut(s) 332, 529
MalI GATC 4 cut(s) 320, 708, 899, 917
MboI GATC 4 cut(s) 318, 706, 897, 915
MboII GAAGA 7 cut(s) 356, 456, 640, 643, 646, 724, 775
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 1 cut(s) 897
MhlI GDGCHC 1 cut(s) 297
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 4 cut(s) 39, 234, 339, 590
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 1 cut(s) 281
Mox20I TGGCCA 1 cut(s) 211
MroXI GAANNNNTTC 1 cut(s) 683
MscI TGGCCA 1 cut(s) 211
MseI TTAA 6 cut(s) 120, 237, 477, 501, 579, 657
Msp20I TGGCCA 1 cut(s) 211
MspCI CTTAAG 1 cut(s) 500
MspI CCGG 3 cut(s) 269, 316, 330
MspR9I CCNGG 5 cut(s) 75, 84, 92, 159, 316
MunI CAATTG 1 cut(s) 339
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 4 cut(s) 75, 84, 92, 159
MwoI GCNNNNNNNGC 2 cut(s) 140, 269
NciI CCSGG 1 cut(s) 316
NdeII GATC 4 cut(s) 318, 706, 897, 915
NheI GCTAGC 1 cut(s) 299
NlaIII CATG 4 cut(s) 14, 52, 207, 805
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 1 cut(s) 805
PcsI WCGNNNNNNNCGW 1 cut(s) 615
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 3 cut(s) 154, 278, 783
PflFI GACNNNGTC 2 cut(s) 413, 749
PfoI TCCNGGA 3 cut(s) 73, 157, 314
PkrI GCNGC 4 cut(s) 401, 425, 507, 778
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 80
Psp5II RGGWCCY 1 cut(s) 80
Psp6I CCWGG 4 cut(s) 73, 82, 90, 157
PspGI CCWGG 4 cut(s) 73, 82, 90, 157
PspN4I GGNNCC 1 cut(s) 88
PspPI GGNCC 2 cut(s) 80, 86
PspPPI RGGWCCY 1 cut(s) 80
PsrI GAACNNNNNNTAC 2 cut(s) 870, 902
PsuI RGATCY 1 cut(s) 897
PsyI GACNNNGTC 2 cut(s) 413, 749
RsaI GTAC 1 cut(s) 912
RsaNI GTAC 1 cut(s) 911
SaqAI TTAA 6 cut(s) 120, 237, 477, 501, 579, 657
SatI GCNGC 4 cut(s) 400, 424, 506, 777
Sau3AI GATC 4 cut(s) 318, 706, 897, 915
Sau96I GGNCC 2 cut(s) 80, 86
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 5 cut(s) 75, 84, 92, 159, 316
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 2 cut(s) 186, 712
SinI GGWCC 2 cut(s) 80, 86
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SpeI ACTAGT 1 cut(s) 115
Sse9I AATT 4 cut(s) 39, 234, 339, 590
SsiI CCGC 3 cut(s) 145, 353, 423
SspMI CTAG 2 cut(s) 116, 300
StyD4I CCNGG 5 cut(s) 73, 82, 90, 157, 314
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 535, 816
TaiI ACGT 3 cut(s) 18, 257, 750
TaqI TCGA 2 cut(s) 384, 564
TasI AATT 4 cut(s) 39, 234, 339, 590
TatI WGTACW 1 cut(s) 910
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 3 cut(s) 154, 278, 783
Tru1I TTAA 6 cut(s) 120, 237, 477, 501, 579, 657
Tru9I TTAA 6 cut(s) 120, 237, 477, 501, 579, 657
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 3 cut(s) 399, 505, 776
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 2 cut(s) 65, 501
TspGWI ACGGA 2 cut(s) 247, 455
Tth111I GACNNNGTC 2 cut(s) 413, 749
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 2 cut(s) 80, 86
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 2 cut(s) 116, 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.