RLG00000029877

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005208
Physical Location & Seq
Forward (+)
50631642 .. 50634023
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029877

Sequence Viewer

Length: 705 bp
ATGACGAACATTATACAAGTTGCCCTTCCGGCTCTGGATTCCCAAGACTCTGAGCACGCTAGCTGGTGCTGTTCCCGGATCGTCGTCTCCGATGACGCAATTGAAGAGGAAGCGGTCGGCGAAGCTTTGCATTCCGATTGTATCGACGGGATTTGGGGCTGCTCCGGGGACACCGTCTACGGCGGCGTGGAGAGAGGTGGTGGAAGATTGAGAAGGTTGCTTCTCCGAATATTGTTTAAGGGGAAGAGAGTTGTTTTCCTTAAGCAGCTTTCATCTGGGTTGCTTCTTGTTACTGTCCTATGTGATTGGCCTTCCACCAAGGTCGATATTTCTGGGGTTAATGTGGAAAAATTTGATGACAAGTATTTCAGAAAGGCCGTGGAAAAGAAGAAGAAGAAGGGGGAGGGAGAGTTTTTTAAGGCAGAGAAAGAGGACAAGAAAGTGTTACCACAAGAGAGGAAAGTTGATCAGAAGAGTGTGGATGCTTCGTTGATAAAGTCCATTGACGTTGTCCCAGACTTGAAGACAAACTTAGCTGCAAGATTCTCTCTGAGGTCTGGCATGTCTTTTAGACAGTCAGCTCTTTTCTTACTTAGGATACTTGTTCCCGTTCTTGTTTGTTTCTCTACTATGATTGTATGTTCTGTTCACATTGAGATCCGAGATTTTGTACTGATCGGCATTGCCTTGCAAGGGATGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L6e Ribosomal_L6e_N

Protein Analysis

235

Amino Acids

26.01

Weight (kDa)

8.43

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L6e PF01159 105 - 190 1.2e-21 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AciI CCGC 2 cut(s) 113, 183
AclWI GGATC 2 cut(s) 86, 652
AcsI RAATTY 1 cut(s) 350
AfaI GTAC 1 cut(s) 672
AfiI CCNNNNNNNGG 1 cut(s) 693
AflII CTTAAG 1 cut(s) 260
AgsI TTSAA 2 cut(s) 104, 523
AluBI AGCT 5 cut(s) 63, 125, 268, 536, 581
AluI AGCT 5 cut(s) 63, 125, 268, 536, 581
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 2 cut(s) 86, 652
AoxI GGCC 2 cut(s) 308, 375
ApeKI GCWGC 3 cut(s) 159, 265, 536
ApoI RAATTY 1 cut(s) 350
AsuC2I CCSGG 2 cut(s) 76, 166
AsuNHI GCTAGC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 146, 277, 523
BceAI ACGGC 2 cut(s) 196, 362
BciVI GTATCC 1 cut(s) 591
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 2 cut(s) 76, 166
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 260
BfuI GTATCC 1 cut(s) 591
BglI GCCNNNNNGGC 1 cut(s) 29
BisI GCNGC 4 cut(s) 160, 184, 266, 537
BlsI GCNGC 4 cut(s) 161, 185, 267, 538
Bme1390I CCNGG 2 cut(s) 76, 166
BmrFI CCNGG 2 cut(s) 76, 166
BmsI GCATC 1 cut(s) 472
BmtI GCTAGC 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 530
BpuMI CCSGG 2 cut(s) 76, 166
BsaJI CCNNGG 3 cut(s) 165, 318, 378
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 681
BseDI CCNNGG 3 cut(s) 165, 318, 378
BseGI GGATG 2 cut(s) 487, 702
BseLI CCNNNNNNNGG 1 cut(s) 693
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 2 cut(s) 42, 542
BseXI GCAGC 3 cut(s) 146, 277, 523
Bsh1285I CGRYCG 1 cut(s) 117
BshFI GGCC 2 cut(s) 310, 377
BsiEI CGRYCG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 3 cut(s) 29, 76, 165
BslFI GGGAC 2 cut(s) 182, 497
BslI CCNNNNNNNGG 1 cut(s) 693
BsmAI GTCTC 1 cut(s) 91
BsmBI CGTCTC 1 cut(s) 91
BsmFI GGGAC 2 cut(s) 182, 497
BsmI GAATGC 1 cut(s) 130
BsnI GGCC 2 cut(s) 310, 377
Bsp1286I GDGCHC 1 cut(s) 57
Bsp143I GATC 4 cut(s) 78, 466, 657, 675
BspACI CCGC 2 cut(s) 113, 183
BspANI GGCC 2 cut(s) 310, 377
BspCNI CTCAG 2 cut(s) 43, 543
BspOI GCTAGC 1 cut(s) 63
BspPI GGATC 2 cut(s) 86, 652
BspTI CTTAAG 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 681
BssECI CCNNGG 3 cut(s) 165, 318, 378
BssMI GATC 4 cut(s) 78, 466, 657, 675
BssT1I CCWWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 175, 295, 576
Bst6I CTCTTC 3 cut(s) 99, 239, 467
BstAFI CTTAAG 1 cut(s) 260
BstC8I GCNNGC 2 cut(s) 57, 61
BstDEI CTNAG 4 cut(s) 51, 532, 551, 593
BstDSI CCRYGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 487, 702
BstKTI GATC 4 cut(s) 81, 469, 660, 678
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 4 cut(s) 78, 466, 657, 675
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 2 cut(s) 74, 164
BstV1I GCAGC 3 cut(s) 146, 277, 523
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 1 cut(s) 657
BstYI RGATCY 1 cut(s) 657
BsuI GTATCC 1 cut(s) 591
BsuRI GGCC 2 cut(s) 310, 377
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 2 cut(s) 487, 702
Cac8I GCNNGC 2 cut(s) 57, 61
CseI GACGC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 671
CviAII CATG 1 cut(s) 562
CviJI RGCY 9 cut(s) 32, 63, 125, 159, 268, 310, 377, 536, 581
CviKI_1 RGCY 9 cut(s) 32, 63, 125, 159, 268, 310, 377, 536, 581
CviQI GTAC 1 cut(s) 671
DdeI CTNAG 4 cut(s) 51, 532, 551, 593
DpnI GATC 4 cut(s) 80, 468, 659, 677
DpnII GATC 4 cut(s) 78, 466, 657, 675
Eam1104I CTCTTC 3 cut(s) 99, 239, 467
EarI CTCTTC 3 cut(s) 99, 239, 467
Eco130I CCWWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 1 cut(s) 565
FaiI YATR 6 cut(s) 14, 301, 563, 632, 640, 703
FalI AAGNNNNNCTT 4 cut(s) 9, 41, 515, 547
FaqI GGGAC 2 cut(s) 182, 497
FatI CATG 1 cut(s) 561
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 4 cut(s) 160, 184, 266, 537
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 4 cut(s) 160, 184, 266, 537
FspBI CTAG 1 cut(s) 60
GluI GCNGC 4 cut(s) 160, 184, 266, 537
HaeIII GGCC 2 cut(s) 310, 377
HapII CCGG 3 cut(s) 29, 76, 165
HgaI GACGC 1 cut(s) 104
Hin1II CATG 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 3 cut(s) 38, 47, 543
HpaII CCGG 3 cut(s) 29, 76, 165
Hpy166II GTNNAC 2 cut(s) 178, 649
Hpy188I TCNGA 8 cut(s) 52, 91, 136, 227, 371, 471, 552, 662
Hpy188III TCNNGA 1 cut(s) 35
Hpy8I GTNNAC 2 cut(s) 178, 649
Hpy99I CGWCG 2 cut(s) 86, 149
HpyAV CCTTC 4 cut(s) 35, 207, 321, 391
HpyCH4III ACNGT 3 cut(s) 175, 295, 576
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 3 cut(s) 130, 539, 691
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 51, 532, 551, 593
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 4 cut(s) 78, 466, 657, 675
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 9 cut(s) 20, 42, 49, 89, 178, 261, 318, 528, 543
Lsp1109I GCAGC 3 cut(s) 146, 277, 523
LweI GCATC 1 cut(s) 472
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 2 cut(s) 289, 444
MalI GATC 4 cut(s) 80, 468, 659, 677
MboI GATC 4 cut(s) 78, 466, 657, 675
MboII GAAGA 8 cut(s) 116, 216, 256, 400, 403, 406, 484, 535
MfeI CAATTG 1 cut(s) 99
MflI RGATCY 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 57
MluCI AATT 2 cut(s) 99, 350
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 6 cut(s) 100, 188, 397, 424, 450, 546
MseI TTAA 4 cut(s) 237, 261, 339, 417
MspCI CTTAAG 1 cut(s) 260
MspI CCGG 3 cut(s) 29, 76, 165
MspR9I CCNGG 2 cut(s) 76, 166
MunI CAATTG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 130
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 2 cut(s) 76, 166
NdeII GATC 4 cut(s) 78, 466, 657, 675
NheI GCTAGC 1 cut(s) 59
NlaIII CATG 1 cut(s) 565
NspI RCATGY 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 38, 543
PflFI GACNNNGTC 2 cut(s) 173, 509
PfoI TCCNGGA 1 cut(s) 74
PkrI GCNGC 4 cut(s) 161, 185, 267, 538
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PsrI GAACNNNNNNTAC 2 cut(s) 630, 662
PsuI RGATCY 1 cut(s) 657
PsyI GACNNNGTC 2 cut(s) 173, 509
RsaI GTAC 1 cut(s) 672
RsaNI GTAC 1 cut(s) 671
SaqAI TTAA 4 cut(s) 237, 261, 339, 417
SatI GCNGC 4 cut(s) 160, 184, 266, 537
Sau3AI GATC 4 cut(s) 78, 466, 657, 675
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 2 cut(s) 76, 166
SduI GDGCHC 1 cut(s) 57
SfaNI GCATC 1 cut(s) 472
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 2 cut(s) 99, 350
SsiI CCGC 2 cut(s) 113, 183
SspI AATATT 1 cut(s) 231
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 2 cut(s) 74, 164
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 3 cut(s) 175, 295, 576
TaiI ACGT 1 cut(s) 510
TaqI TCGA 2 cut(s) 144, 324
TasI AATT 2 cut(s) 99, 350
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 2 cut(s) 38, 543
Tru1I TTAA 4 cut(s) 237, 261, 339, 417
Tru9I TTAA 4 cut(s) 237, 261, 339, 417
TseI GCWGC 3 cut(s) 159, 265, 536
TspDTI ATGAA 1 cut(s) 261
Tth111I GACNNNGTC 2 cut(s) 173, 509
Vha464I CTTAAG 1 cut(s) 260
XapI RAATTY 1 cut(s) 350
XceI RCATGY 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.